LOCUS       DASJTM010000001        81238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJTM010000001 DASJTM010000000
VERSION     DASJTM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.001"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..1064
                     /locus_tag="U4B29_000001"
     CDS             90..1064
                     /locus_tag="U4B29_000001"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2104982.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1234..2634
                     /locus_tag="U4B29_000002"
     CDS             1234..2634
                     /locus_tag="U4B29_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN2104983.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2712..3434)
                     /locus_tag="U4B29_000003"
     CDS             complement(2712..3434)
                     /locus_tag="U4B29_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2104984.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3513..4019)
                     /locus_tag="U4B29_000004"
     CDS             complement(3513..4019)
                     /locus_tag="U4B29_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2104985.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4142..5941
                     /locus_tag="U4B29_000005"
     CDS             4142..5941
                     /locus_tag="U4B29_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN2104986.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6009..7619)
                     /locus_tag="U4B29_000006"
     CDS             complement(6009..7619)
                     /locus_tag="U4B29_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2104987.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(7874..8383)
                     /gene="mreD"
                     /locus_tag="U4B29_000007"
     CDS             complement(7874..8383)
                     /gene="mreD"
                     /locus_tag="U4B29_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN2104988.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(8387..9241)
                     /gene="mreC"
                     /locus_tag="U4B29_000008"
     CDS             complement(8387..9241)
                     /gene="mreC"
                     /locus_tag="U4B29_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN2104989.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(9396..10034)
                     /locus_tag="U4B29_000009"
     CDS             complement(9396..10034)
                     /locus_tag="U4B29_000009"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN2104990.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(10035..10685)
                     /locus_tag="U4B29_000010"
     CDS             complement(10035..10685)
                     /locus_tag="U4B29_000010"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN2104991.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(10698..11597)
                     /gene="rsgA"
                     /locus_tag="U4B29_000011"
     CDS             complement(10698..11597)
                     /gene="rsgA"
                     /locus_tag="U4B29_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN2104992.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(11762..13831)
                     /gene="pknB"
                     /locus_tag="U4B29_000012"
     CDS             complement(11762..13831)
                     /gene="pknB"
                     /locus_tag="U4B29_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN2104993.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(13828..14568)
                     /locus_tag="U4B29_000013"
     CDS             complement(13828..14568)
                     /locus_tag="U4B29_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN2104994.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(14581..15939)
                     /gene="rsmB"
                     /locus_tag="U4B29_000014"
     CDS             complement(14581..15939)
                     /gene="rsmB"
                     /locus_tag="U4B29_000014"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN2104995.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(15939..16877)
                     /locus_tag="U4B29_000015"
     CDS             complement(15939..16877)
                     /locus_tag="U4B29_000015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN2104996.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(16870..17361)
                     /gene="def"
                     /locus_tag="U4B29_000016"
     CDS             complement(16870..17361)
                     /gene="def"
                     /locus_tag="U4B29_000016"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2104997.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(17376..19784)
                     /gene="priA"
                     /locus_tag="U4B29_000017"
     CDS             complement(17376..19784)
                     /gene="priA"
                     /locus_tag="U4B29_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN2104998.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(20037..20342)
                     /locus_tag="U4B29_000018"
     CDS             complement(20037..20342)
                     /locus_tag="U4B29_000018"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2104999.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(20347..20961)
                     /gene="gmk"
                     /locus_tag="U4B29_000019"
     CDS             complement(20347..20961)
                     /gene="gmk"
                     /locus_tag="U4B29_000019"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN2105000.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(21448..21732)
                     /locus_tag="U4B29_000020"
     CDS             complement(21448..21732)
                     /locus_tag="U4B29_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105001.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(21750..22640)
                     /locus_tag="U4B29_000021"
     CDS             complement(21750..22640)
                     /locus_tag="U4B29_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN2105002.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(22660..22878)
                     /locus_tag="U4B29_000022"
     CDS             complement(22660..22878)
                     /locus_tag="U4B29_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN2105003.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(22848..24548)
                     /locus_tag="U4B29_000023"
     CDS             complement(22848..24548)
                     /locus_tag="U4B29_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN2105004.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            24815..25588
                     /locus_tag="U4B29_000024"
     CDS             24815..25588
                     /locus_tag="U4B29_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105005.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(25669..26238)
                     /gene="thiT"
                     /locus_tag="U4B29_000025"
     CDS             complement(25669..26238)
                     /gene="thiT"
                     /locus_tag="U4B29_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN2105006.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(26304..26406)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(26412..27383)
                     /locus_tag="U4B29_000026"
     CDS             complement(26412..27383)
                     /locus_tag="U4B29_000026"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2105007.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(27477..28850)
                     /gene="glmU"
                     /locus_tag="U4B29_000027"
     CDS             complement(27477..28850)
                     /gene="glmU"
                     /locus_tag="U4B29_000027"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN2105008.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(29353..30171)
                     /gene="purR"
                     /locus_tag="U4B29_000028"
     CDS             complement(29353..30171)
                     /gene="purR"
                     /locus_tag="U4B29_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN2105009.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(30338..31162)
                     /locus_tag="U4B29_000029"
     CDS             complement(30338..31162)
                     /locus_tag="U4B29_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2105010.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(31135..31815)
                     /locus_tag="U4B29_000030"
     CDS             complement(31135..31815)
                     /locus_tag="U4B29_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2105011.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(31838..32776)
                     /locus_tag="U4B29_000031"
     CDS             complement(31838..32776)
                     /locus_tag="U4B29_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2105012.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(33021..33884)
                     /locus_tag="U4B29_000032"
     CDS             complement(33021..33884)
                     /locus_tag="U4B29_000032"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN2105013.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(34026..36599)
                     /gene="mprF"
                     /locus_tag="U4B29_000033"
     CDS             complement(34026..36599)
                     /gene="mprF"
                     /locus_tag="U4B29_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2105014.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            36740..37546
                     /gene="proC"
                     /locus_tag="U4B29_000034"
     CDS             36740..37546
                     /gene="proC"
                     /locus_tag="U4B29_000034"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN2105015.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(37625..39181)
                     /gene="rny"
                     /locus_tag="U4B29_000035"
     CDS             complement(37625..39181)
                     /gene="rny"
                     /locus_tag="U4B29_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN2105016.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(39592..40641)
                     /gene="recA"
                     /locus_tag="U4B29_000036"
     CDS             complement(39592..40641)
                     /gene="recA"
                     /locus_tag="U4B29_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN2105017.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(40750..41991)
                     /locus_tag="U4B29_000037"
     CDS             complement(40750..41991)
                     /locus_tag="U4B29_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN2105018.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(42231..42809)
                     /gene="pgsA"
                     /locus_tag="U4B29_000038"
     CDS             complement(42231..42809)
                     /gene="pgsA"
                     /locus_tag="U4B29_000038"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN2105019.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(43018..43941)
                     /locus_tag="U4B29_000039"
     CDS             complement(43018..43941)
                     /locus_tag="U4B29_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105020.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(44680..45966)
                     /locus_tag="U4B29_000040"
     CDS             complement(44680..45966)
                     /locus_tag="U4B29_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2105021.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(45959..47227)
                     /locus_tag="U4B29_000041"
     CDS             complement(45959..47227)
                     /locus_tag="U4B29_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2105022.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            47479..47940
                     /gene="mscL"
                     /locus_tag="U4B29_000042"
     CDS             47479..47940
                     /gene="mscL"
                     /locus_tag="U4B29_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN2105023.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(47999..48445)
                     /locus_tag="U4B29_000043"
     CDS             complement(47999..48445)
                     /locus_tag="U4B29_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105024.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(48538..48783)
                     /locus_tag="U4B29_000044"
     CDS             complement(48538..48783)
                     /locus_tag="U4B29_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN2105025.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(48898..49476)
                     /locus_tag="U4B29_000045"
     CDS             complement(48898..49476)
                     /locus_tag="U4B29_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2105026.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(49553..50083)
                     /locus_tag="U4B29_000046"
     CDS             complement(49553..50083)
                     /locus_tag="U4B29_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN2105027.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(50087..50947)
                     /gene="rlmB"
                     /locus_tag="U4B29_000047"
     CDS             complement(50087..50947)
                     /gene="rlmB"
                     /locus_tag="U4B29_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN2105028.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(50940..51335)
                     /locus_tag="U4B29_000048"
     CDS             complement(50940..51335)
                     /locus_tag="U4B29_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN2105029.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(51332..52744)
                     /locus_tag="U4B29_000049"
     CDS             complement(51332..52744)
                     /locus_tag="U4B29_000049"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN2105030.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(52741..53277)
                     /gene="cysE"
                     /locus_tag="U4B29_000050"
     CDS             complement(52741..53277)
                     /gene="cysE"
                     /locus_tag="U4B29_000050"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN2105031.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(53363..53602)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(53599..55059)
                     /locus_tag="U4B29_000051"
     CDS             complement(53599..55059)
                     /locus_tag="U4B29_000051"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN2105032.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(55229..56386)
                     /locus_tag="U4B29_000052"
     CDS             complement(55229..56386)
                     /locus_tag="U4B29_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN2105033.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(56420..57790)
                     /gene="radA"
                     /locus_tag="U4B29_000053"
     CDS             complement(56420..57790)
                     /gene="radA"
                     /locus_tag="U4B29_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN2105034.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(57853..58380)
                     /locus_tag="U4B29_000054"
     CDS             complement(57853..58380)
                     /locus_tag="U4B29_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN2105035.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            58610..59416
                     /gene="proB"
                     /locus_tag="U4B29_000055"
     CDS             58610..59416
                     /gene="proB"
                     /locus_tag="U4B29_000055"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN2105036.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            59413..60660
                     /locus_tag="U4B29_000056"
     CDS             59413..60660
                     /locus_tag="U4B29_000056"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN2105037.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            60736..61080
                     /locus_tag="U4B29_000057"
     CDS             60736..61080
                     /locus_tag="U4B29_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105038.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(61197..61523)
                     /locus_tag="U4B29_000058"
     CDS             complement(61197..61523)
                     /locus_tag="U4B29_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105039.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(62138..63340)
                     /locus_tag="U4B29_000059"
     CDS             complement(62138..63340)
                     /locus_tag="U4B29_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2105040.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            63457..63972
                     /locus_tag="U4B29_000060"
     CDS             63457..63972
                     /locus_tag="U4B29_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2105041.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(64171..64617)
                     /locus_tag="U4B29_000061"
     CDS             complement(64171..64617)
                     /locus_tag="U4B29_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2105042.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(64664..66142)
                     /gene="msr(C)"
                     /locus_tag="U4B29_000062"
     CDS             complement(64664..66142)
                     /gene="msr(C)"
                     /locus_tag="U4B29_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2105043.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(66496..67125)
                     /locus_tag="U4B29_000063"
     CDS             complement(66496..67125)
                     /locus_tag="U4B29_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2105044.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(67109..67573)
                     /locus_tag="U4B29_000064"
     CDS             complement(67109..67573)
                     /locus_tag="U4B29_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2105045.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(68060..69328)
                     /locus_tag="U4B29_000065"
     CDS             complement(68060..69328)
                     /locus_tag="U4B29_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2105046.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(69559..70296)
                     /locus_tag="U4B29_000066"
     CDS             complement(69559..70296)
                     /locus_tag="U4B29_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2105047.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(70363..70932)
                     /locus_tag="U4B29_000067"
     CDS             complement(70363..70932)
                     /locus_tag="U4B29_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2105048.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            71060..71932
                     /locus_tag="U4B29_000068"
     CDS             71060..71932
                     /locus_tag="U4B29_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2105049.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            72274..73146
                     /locus_tag="U4B29_000069"
     CDS             72274..73146
                     /locus_tag="U4B29_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2105050.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            73143..75101
                     /locus_tag="U4B29_000070"
     CDS             73143..75101
                     /locus_tag="U4B29_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2105051.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(75433..76284)
                     /locus_tag="U4B29_000071"
     CDS             complement(75433..76284)
                     /locus_tag="U4B29_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2105052.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            76407..76874
                     /locus_tag="U4B29_000072"
     CDS             76407..76874
                     /locus_tag="U4B29_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN2105053.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(77137..77619)
                     /locus_tag="U4B29_000073"
     CDS             complement(77137..77619)
                     /locus_tag="U4B29_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN2105054.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(77719..78582)
                     /locus_tag="U4B29_000074"
     CDS             complement(77719..78582)
                     /locus_tag="U4B29_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2105055.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            79180..80055
                     /locus_tag="U4B29_000075"
     CDS             79180..80055
                     /locus_tag="U4B29_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105056.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(80200..80352)
                     /locus_tag="U4B29_000076"
                     /pseudo
     CDS             complement(80200..80352)
                     /locus_tag="U4B29_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(80620..>81238)
                     /locus_tag="U4B29_000077"
     CDS             complement(80620..>81238)
                     /locus_tag="U4B29_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2105057.1"
                     /translation="TGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQNMQENMA
                     AYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLSVTPAIF
                     NISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWTTPPVIS
                     GFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        23161 a        17264 c        14591 g        26222 t
ORIGIN      
        1 tattttatag ccaaatagcg tgacatgggg cttgagaaat gttagcataa gcatgggaaa
       61 aaacaaatca atggaaggat tgatactaaa tgactgcagc tgcagaaaaa aaagatcatc
      121 aaaaagttat tcttgttgga gacggcgccg taggttctag ctatgccttt gctctagtta
      181 ctcaaaatat cgctcaagaa gtaggtattg tggatatcaa tactgctaaa actgaaggag
      241 acgcaattga cttgtctcac gctttggcat ttacttcacc taaaaaaatc tattctgcta
      301 cttacgcaga cgcacatgat gctgacttag ttgttattac agctggcgca ccacaaaaac
      361 caggtgaaac tcgtttagac ttagtaaaca aaaacctaaa aatcaaccgc gatgttgtca
      421 cacaaatcgt tgaatcagga tttaacggta tctttttagt tgccgctaac ccagtagata
      481 ttttgactta ctcaacttgg aaattctcag gtttccctaa agaacgcgta atcggttcag
      541 gtacctctct tgactcagct cgtttccgtc aagcacttgc ggaattagtt gacgttgatg
      601 cacgtaacgt ccatgcttac atcttaggtg aacacggaga ttcagaattc ccagtttggt
      661 cacatgcaaa tgttgccggc ttgcaaatct atgaatgggt aaaaaacaac ccagaaatcg
      721 atgaagaagc tatggttaac ctattctttg gcgtacgtga cgctgcttac acaattatcg
      781 agaaaaaagg cgctactttc tatggtatcg cagtagcatt agcacgtatc actcgtgcca
      841 tcttagatga tgaaaatgct gttcttcctt tatcagtatt catgaatggc gaatatggat
      901 tgaacgacat ctacattggt gcacctgctg ttatcaaccg tcaaggtatc caaaaagtaa
      961 tcgaaattcc tttaagtgac agcgagcaag accgtatggc tgcttctgct aagcaattaa
     1021 aagaaattct agatgaagct tttgctaaac tagacgctga ataatctttg aacatgacgg
     1081 aaaacatcta ttcggtgttt tccgtttttt tattttatca cccttatggt ttctaaattt
     1141 tttcataaag tagagataac cttcgtcttt tcctctaaat tatgctatca tgagaccgtt
     1201 ccaccctaaa cttttactta aggagttgtc gatatgacaa gatcttcgca tcgaaaaaaa
     1261 tcgaaacatg ttgctttgct ttcagttctt ggtgttctct tagtgctgat cagcttttat
     1321 acctatcgca gtacatacta cacaaagcat tttttgccga atacagaaat aaatggaata
     1381 aatgtcagca atctaacagt cgaaaaagca aatgaaaaat taaaagaagc ttattctgat
     1441 aaaaaactgg ctattaaaga agatggaaaa gtctggcagg aagtaccaaa atccgagctc
     1501 ggctacaaag atgatttcac ttcggagctt tctccgatcc taaatgaaca aaacggatgg
     1561 acttggggaa tgacttatgt ctcagcggca gaaaaacaag aaatagatcc tgtcagtcaa
     1621 aaccaccaaa aactagacac taccgttcag acattgacaa ctaagctgac tgatttgaac
     1681 aaagaccgga cacctactac tgatgcaacg atcgaaaaag caggtgactc tttcgttatc
     1741 aagcctgaag tcaatggaaa tacgatcgac gtcgatgctg cggtcaaaca attaaaaagt
     1801 gcggtaaact ctggtaaaga tacaattgaa ttgactgaat tcaaagaaaa accaaaagta
     1861 acatctgaag acagctcact gaaagaacaa ctcgcatcca tgaatgctat cgcaaatgta
     1921 aaagctactt acagtataaa cggtgagact ttccagatcc catcaagtga tattatgagt
     1981 tggctgacct acaatgatgg aaaagtcgat ttagatacag aacaagtacg tcaatatgta
     2041 acagatttag gcacaaagta taacacaagt acgaatgaca caaaatttaa aagtacaaaa
     2101 agaggagaag ttacggtccc agttggcact tacagttgga cgatccaaac agacagtgag
     2161 acagaagcat tgaaaaaagc gattctggca ggccaagatt ttactcgttc acccattgtg
     2221 caaggtggca caaccgctga tcatccattg atcgaagata cgtatatcga agttgatctg
     2281 gagaaccagc atatgtggta ttacaaagac ggaaaagtgg cccttgaaac agatatcgtt
     2341 tccggaaagc caactacacc tacacctgct ggagtcttct atgtttggaa taaagaagaa
     2401 gatgcaactt taaaaggaac aaatgacgat ggaacacctt acgagagtcc ggtaaactac
     2461 tggatgccga ttgactggac aggtgttggt atccacgatt cagactggca acctgaatac
     2521 ggtggagact tatggaaaac ccgcggttca cacggctgca tcaacacccc accaagtgta
     2581 atgaaggaat tattcgggat ggtcgaaaaa ggaacgcctg tattggtctt ctaacaaaaa
     2641 taatatataa aaagaaagca cccgtacgat tggtacaggt gctttctttt tattatctaa
     2701 caatgtgcta cttacatttc agcagcagta aagacttctg aaacatcatc atcgtcttct
     2761 agcttatcta ctagacgttc caattgctct ttttgttcct catttaaatc aatcgtcgtt
     2821 tgtggaatca tcgtcaattc tgcttgcgcc aaagtgaact ctttttccaa ctcatctcgt
     2881 acagcagcaa aatcttctgg tgcagtataa atctcgaata cttcaggaga agtttgcaga
     2941 tcttctgccc ccgcctctaa tacatcctca aacatcgtct cttcatcaac agtcaaccca
     3001 tcgcgttcaa tagcgatata tcctttgcgg tcaaacaagt aactgactga acccgtttct
     3061 cccagtgatc caccgtttcg agtaaaagct actcggacat ttgtcgctgt ccgattacgg
     3121 ttatctgtca atgcatggac taagattcct actccacccg gaccatatcc ttcatacgtg
     3181 atttcatcgt aattcgtttc atctacggca ctcgtagcct ttttaatcgc ccgttccaca
     3241 ttgtcgttag gcatatttgc agcttttgct ttgtccacaa caagccgcaa agctggattc
     3301 atagatggat caggaccgcc tgctttagct gccatataaa tttcacgaga aagtttctga
     3361 aagactttcc ctcgttttgc atcttgggca ttttttcttc cttggatatt atgccattta
     3421 ctatgtcctg acatgatttt tcctcccttg ttcttttgag aatacacttc tattctagta
     3481 ctatttcaca aaaaaacaaa gccacagcca atctataaac gcttcttgaa tgccgcaaaa
     3541 agcacacata atatcacagc agtcagagca ccgatcgaat ccaacatgac atcttggaac
     3601 ataggtgttc gtccaccggt catcatctga tggaattcgt ccaaacctgc gtaccctgtt
     3661 gctgaaagcc aagcaaggat cgctgtcagc caccaaattt ttagcttggg gaaaacacct
     3721 aaaaacaagc tgcctcccaa aataaaatag gtaccgaaat gtgcgccctt ccttaaaaag
     3781 aactctaaaa atttggcgta gcccagatgg ttgatactca ccatacttcc accataatta
     3841 aaacgtatac ccgctaatgt gtctttaaat ggctgattag gcagccagct ttctaatcgc
     3901 gaaacttgtg attgctgtcc gtaagtttga gaagaactgt aaaataagat tataacgact
     3961 atcagtgcga ttactaaata aatatttccg tttttcattt gtttttttga agaattcata
     4021 agtcacctct gtattttatc ttagactttt tttgaagaaa caacaatcat tttcatagag
     4081 acttcttcca taaaaaagtg ctatacttaa ttttgtatta caatatgaag ggagtttttt
     4141 catgagttat tcattaaatt ggaatctaga ttctattttt tccggaggca gtcattctga
     4201 tgctttgaac caacggatga agcaattgga agaccaaatg aacgagtatt atcaccgcgt
     4261 aacaaaatgg tcacctagtt ctgacaatgc agagcagtta aacgctattc ttcaattgca
     4321 agaaacgatt acgaatggtt ttacccaatg cagcagctat attactgcat tgctttctgc
     4381 caatgtcaat gattcagatg caaaggtctt atcaggcaag ctttacgcaa tgctgcctcg
     4441 tctgcaatca gccgaaactg tattgtcaaa gaaatttgca gaaatatctg acaatgattg
     4501 gaatcagatg ctttctcaat cttcgtttga aacaattgct tttcgtttaa acgagattcg
     4561 tcgtgatggc tcacaattat tatcagaagc tgaagaaaac attataaata cgctttcttt
     4621 agatggtcta aatgcttgga gcagtcatta tgatacgatt gtagccagca tttcgattcc
     4681 cttcgaacaa gatggacaag tagtcgaatt atctgctgga caagccttca ataagatgat
     4741 gggcgatcca gatccaaaag tcagagaaac acttttcgca gcatgggaag aagcctggaa
     4801 agaaaaggca cccctcttca cggatacttt gaatcattta gatggtttta gactttctga
     4861 ctatgaactt catggtgtca cagattttct acaaaaacca ttagagtaca atcggttgaa
     4921 gaaagaaaca ttgtctgtca tgtgggatac aatccagaaa aacaaacaac cccttgttaa
     4981 ttacttgact agaaaagcta atcttttcgg taaagaaaaa atggaatggc aagatcaaga
     5041 tgcacctatt attttaggcg atttgaaaga aaaaaccttt agttttgacg aagcagcagc
     5101 atttattatc gaaaacttta acaaattcag tccaaaaatg gctaaatttg cccaatctgc
     5161 ttttgaaaag agctggatcg aagcagaaga tcgtcctggg aaacgtcctg gtggttattg
     5221 tactgagtta ccagaaacaa aagaatcaag gatctttatg acctacagta attctgttaa
     5281 tgaggttgcg acacttgctc atgaactagg acatgctttc catagcagca cgatgtggga
     5341 tttaccttct ttaaatcgcg aatatgccat gaatgtcgca gaaacagcaa gtacattcgc
     5401 agaactgatc gtagccgatg ctacattaaa agtcgcaaaa acaaaagaag aaaaaatcaa
     5461 tttgctagat acaaaactgc aaaatgcttt agctatgttc atgaatatcc attcccgatt
     5521 catttttgaa aatcgattct atgaagcacg gcaagaagga ttagtctcag aagataagat
     5581 cacagagatg atggttgagg cgcaaaaaga aggctatcaa ggagctttag ctacttacca
     5641 tccatatttc tgggctgcaa aattgcattt ctttattgat gacgtaccgt tttataactt
     5701 cccatacaca tttggctacc tctttagttt gggcatctat gcttacgcca accaaaaggg
     5761 agcaaatttt gaagaagaat atatccattt actacgcgat actgcctcaa tgacgacgga
     5821 agatcttgcg aagaaacatt tgaatgtcga tcttacccaa ccagattttt ggcaagcagg
     5881 tatcgacatg gtgactgaag atatccaaac gttcatggaa ttgacagaag aatttatctg
     5941 atttctattt tatcacaata aaaaaagagt tagtccagaa tgggctaact ctttttgatt
     6001 aatcattctt acatgctgac agcaaagtca ggggcaaacc attgaactga accaactttt
     6061 acgctttcgc ctggttgagg agcatggata tattgtcctc cgcctaaagc aatcgctacg
     6121 tggtaagttc cgccttgtga cccccagaat aacaagtctc cagcttttgc ttgagaaact
     6181 gaaatttttg ttccagctga ttcttgagga actgtccaac caccgatgtc acgaccagtt
     6241 acttgcatgt aaacatagcg tgtgaaccct gagcagtcaa agccacttgg atctttaccg
     6301 ccccaaacat atggagtacc aatatatttg taagcttctg ctacgattgc tgcaccattc
     6361 acacttccag aaggagctgg agcaggtgtt acaggttttg gttgttcagg tgttggtgtt
     6421 ggcgttggag ccggagtact acttcctgtg ccgtttcctg tatcaacact ttgatctgtt
     6481 gatggtgttg taggcgcagg tgttgttgat tcttctgttg ttgaagattc aggcactgtt
     6541 gttgattctt ctgttgctga agattcaggc actgttgttg attcttctgt tgctgaagat
     6601 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
     6661 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
     6721 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
     6781 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
     6841 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
     6901 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
     6961 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
     7021 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
     7081 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
     7141 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
     7201 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
     7261 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
     7321 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
     7381 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
     7441 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
     7501 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
     7561 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
     7621 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
     7681 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
     7741 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
     7801 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
     7861 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
     7921 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
     7981 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
     8041 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
     8101 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
     8161 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
     8221 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
     8281 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
     8341 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
     8401 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
     8461 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
     8521 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
     8581 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
     8641 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
     8701 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
     8761 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
     8821 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
     8881 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
     8941 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
     9001 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
     9061 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
     9121 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
     9181 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
     9241 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
     9301 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
     9361 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
     9421 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
     9481 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
     9541 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
     9601 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
     9661 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
     9721 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
     9781 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
     9841 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcacccact
     9901 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
     9961 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    10021 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    10081 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    10141 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    10201 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    10261 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    10321 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    10381 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    10441 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    10501 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    10561 tttgggacaa atcggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    10621 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    10681 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    10741 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    10801 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    10861 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    10921 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    10981 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    11041 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    11101 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    11161 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    11221 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    11281 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    11341 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    11401 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    11461 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    11521 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    11581 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    11641 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    11701 acaaaaaaga gaatgcaaca aatcaatgtc gcattccctt ctgatatatt ttattcagca
    11761 cttattttga actttctgag gtgctagctg atgaacttgt ggagctggaa gattcttctt
    11821 ttgaagagtt gcttgtgcta ctggagctac ttgagctgct agaactgtct gaagagtctt
    11881 tttcagaact agaagacgct ggatctggtc ctttagagta aattacgttc acaaccattc
    11941 ctttggtaat tgtcgttcct gcccctggta ctgtacgaat cactttatct ttatccaccg
    12001 tgtttgaata ttcggaagtc tcatgcccga tatattctgc accgacacta gctaaataac
    12061 tttgtgcttc gctttttgta tagccggaga tatcattcaa tttaggctct gttccttggc
    12121 ttacgacaaa attgatgtaa gtttcttctg ctacaacttc acccgaggct ggatcctgac
    12181 tgatgatcga acctgatggt acagtgtcgc tatagtcata agtagcacta atattattga
    12241 tattgaatcc ttgtgtaaca agccaattac gaacagtttc gtagttttct cctacatgac
    12301 tactcaaagt cactgtttct ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag
    12361 gatcgacttc tgtatcttct gatggagatt gcgaaatgat cattccgctt tctacctctg
    12421 aactgaattc ctcttctttt tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg
    12481 cttcggcatc ttcataactt ttaccactgt aatcatctaa tttgattttt ttcgtgccgc
    12541 tactaatata cagagtgact tcgcgttttt gcttgactgt cgttcccgct gccgggtctg
    12601 tcttcactac attaccttct tcgattttgt catctgcaat ttcttctgtt tttgttgcta
    12661 cttctaattt cgcttttgcg agtgtttctc tagcagaagc ttcagaaaga tcagaaacat
    12721 caggaacaac aacttctcct cgcccgccag aagcaaagaa tacagctgtt ccgattccta
    12781 aaacagcaag aatagctaat aggatcagcc accatttttt ctgtcctttc tttttaggat
    12841 caggcagaac ttcttcctcc tctggtaagg tttcgttttc tggcttgtct tttggcagag
    12901 gcatagattt gaatgaatca ggcattggtg catcttccgt aaggggtgtg ataacttttg
    12961 tttcattatc catcacatga ggctgccatt tttcttcgtt tgcacgagct ggtgataata
    13021 ctgtagaaag atcatcagac atttcctcgg ctgacttata acggtctgca ggttctttag
    13081 ccgttgcccg taaaatcacg ttttccagtg cttgtggcac atttggatca agtgctttta
    13141 tagatggaag atcatcttga aaatgtttta aagcaattgt tacagcagat tcaccatcaa
    13201 acggtacgct acccgtcaac atctcataaa ggatgatccc taaagcataa atatctgact
    13261 gcttcgttgc catacttcct ctggcctgct caggggacag ataatgaaca gatcctagca
    13321 tcgtattcgt ctgcgtgatc gatgtttcag ataaagcgat agcaatcccg aaatccgtga
    13381 tttttacaac accttcatta tcgatcagta cgttttgggg tttcaaatct cggtggatga
    13441 tttgatggtt atgagctaaa gaaatagcag aaaggatctg ctgcataata tttactgctg
    13501 tttcataagg aacaggataa tgtgtttgga tatatcgttt caagtccatt cctttgacgt
    13561 attccatcac cagatactgc atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt
    13621 ttggatgaac gagttctgtt gctgctaaag cttctctctg aaatcgtcga atcgcagtct
    13681 ggtcattttg aaagtcaaat cgtaagactt taactgctac atcacgatcc aagattaaat
    13741 cgtgcgccaa aaatacattt gccataccac cgctaccgat atttccagtg atttgataac
    13801 ggccatttaa ttttttgccc aactcgatca tacactgtcc tccttatatt cgataagaag
    13861 aacggtgata ttatccgctc cgcctgcttc attggcacga tcaatcagct cagttacttt
    13921 atgagacaat gggccttctt gattgatgat cgttcctatt tcttcttcag acagcatgtt
    13981 cgtcaagcca tctgaacata acagcagtcg atcttttagt tgccaaagat gagtagaaac
    14041 atctacttcg actgttccag gcatgccgac agaacgtgtt aaaatatttt tccttggatg
    14101 gttcactgcc atttcttcgc tgatttctcc tgatttgact agttcattga ctaaagaatg
    14161 atcctccgtc aattgcatga ttttatcttc acgaatcaaa taagctctgc tatccccaac
    14221 attggcaatc gtgaaccagt catctgacag agcggccgct acgattgttg ttcccatacc
    14281 gttatattcc ggctgttctt gtccacgctg ataaatcagc atattttctt cttgaatcga
    14341 ttgaatgaac cattgggcga ttttttcatc gtcaacagct tcttgttttt cccaagcttc
    14401 gcctagattc gttaccgcca tctgactggc aatgtctccg gcacgatgac cacccatccc
    14461 gtctgctaat acagcaagct tgagacctga acggctggta aatacactgg catagtcttg
    14521 atttgtgtta cgtcgtcttc ctacatctga ttgatattca atctgcattt tttcacctca
    14581 ttactttttg cgcaaacaac aaatgaaaaa tccatctgtg taaaactgat gcggatacaa
    14641 agtcaacatt ttctcttcga ttgatgattg tagcatagga ttggtcaaaa catcaatcct
    14701 ctcaaaatca gggtgtcttt ccaagaaagc agaaacaacg tcttggtttt cttcttttaa
    14761 gatcgtacaa gtgctataag tcagaatacc ggaagacttt aaagttggtg cgcagctttc
    14821 taaaatttcc aattggattt ttggtaaatt cgctaattca tccggctgtt ttttataacg
    14881 aatatctggt tttcgccgaa gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg
    14941 atcaaactgc tctgcttcga actgtttact gacttctcta gcatccatct tttgagcgta
    15001 gacagcagaa gagacaccta gacgtgctgc attatcttca atgagtttga ctttgtgttc
    15061 gtgtacatct aaagcgacca cttctccacc gcatgacgga tctaaaaaag aagcaatatg
    15121 cgtcgtctta cctccgggtg ctgcacaggc atctaagaca tgatgttcag gctcgatttg
    15181 catagacggc gctacaagca tcgaactttc atcttggatc gtcatgacgc catcgtggaa
    15241 caatgagcta gaagctaaat gccctttttt cgctactata ccgtatggac tgacttggct
    15301 tttttcagct gtgatctctt cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg
    15361 gcgtgtatct actcgggcac tagcatggct aggctgatat agcgataaac cgagcttttc
    15421 agtttcttcg tatccgattt gatccaccag tcgtttcgtc atccatagag gcaggctgat
    15481 tttgacagaa agtctttcga taggatcagc aatgccttgc agatctggtg ctccttgacg
    15541 ctgataattc ctcaagacac catttacaaa tttccctgtc ccaggatttc cttttacttt
    15601 tgcgatttcg accgcctcgt ttaaaatagc atgtgcaggg actttatcta aaaattctaa
    15661 ttggtataca gaaagataaa gaagcatctt gacccagtca tccacttttt ttgctttagc
    15721 gatcaatggt gcaacgtaaa attccaatag tcgctgatgg ctaatcgtac catagatcaa
    15781 ttctgtaaat aaacccacat ctttttgatt tagctcgctt tgtttgatca tctcattcaa
    15841 caaaagattc gagtaggcgc cacccttgtt cactcgctct aatgtcagta atgccatata
    15901 tcgtactgat tttttcattt ttttagggat ttttttcatt atttcacctg ctgaccaatc
    15961 tgaacttgct gaccgcttcc gttaaggaaa tcatgtacgg cctgttttcc tttaccagct
    16021 ggctgtaatt cattgatggc taagacggtc tgtttaccgc aagcaatcca tagatttttt
    16081 ttgtctttcc ggatgatcgt accaggtgct ttgtctgtag tttctgccaa tacttcaacc
    16141 gataataatt tccagcgtat gccctcatag gtcgtgaatg caattggcca aggacgcatt
    16201 ccgcgtactt ggttgtcaat ttcttctgca gttttatgcc aatcgattgc ttcttgttcc
    16261 cgtgtgatgt ttggcgaaaa agttgcttct gactcgtctt gaggtactgg tttttgtcca
    16321 tccaaaatat tgggcaatgt ttctagcaat agctttcttc ctaaaaggct aagcttttca
    16381 aacatcgtcc ctacatcatc ttgtttagtg attggtatgc tttcttgcgc atagattccg
    16441 cctgcatcca tttttttgat catttccatg atcgttacac cagtttcttt ctccccattc
    16501 atgatggcat aatgaacagg cgcacctccc cgatatttag gaagtaaaga agcatggaca
    16561 ttgattgccc ctaattttgg tacttgcaat aacttttcag gcaagaattg gccaaatgcc
    16621 gcagtgacga tcacatcagg caccaattca atgatttctt ccatttctgg cgaaccggag
    16681 attttctctg gctgcagcac acggataccg tgcttcagag ccgctttttt taccggtgtc
    16741 ggcgtaatta ctcgtttccg cccaactgga cggtcgggct gtgttaccac tgcctggatc
    16801 tcgtaacctg attctatcag cccttcaaga atcggcacag aaaaagcagg tgtgcccata
    16861 aatactagct tagtcatcaa gatgttcctc catatattct tcaagttctt cttccgggat
    16921 ctgctgtatc attttctcaa tgaacaagat accgtctaag tgatcgattt catgctggaa
    16981 ggcacgggct aaatagccaa aagccgtcac ttctatctct tctccgtctc gatcataata
    17041 acgcacggtc acttcatctg ctcgcttgac cgttccatag acatgcggaa tactgagaca
    17101 accttctacg tcaaggcttt caccttttgc ttcgatgatt tctgggttaa tcagttcaaa
    17161 tttctcgcct tcatctactt cgatcacagc aattctcttg ttttgtccta cttgaggagc
    17221 agcgatccct attccgtcat tcgcaatcat tgtctcatat aaattgtcta ataaagcgat
    17281 tgtctcgtcc gtgattacat cgattggctg agcagttctt tttaatttat cattagggtg
    17341 gatcaatata ggatagcgca taatttggcc ctttcttaaa taaagtttaa tggttcgttg
    17401 tcaattgcca caaaaagacc ttgtcgctgt tctttctggc ttccattcaa aatttcatcc
    17461 aaaacatgag gaagattcgg ttcatgttta tatttgatga ttagttgata ataatatcgg
    17521 tttttcactc tcaaaatcat gctgggcgtt ggccctaata gcaagctttg agggcttaac
    17581 acttctttta gctgattggc aatctgaaag attttctttg ccgctacttg ttcttcagga
    17641 tggctggcgg ttattttcac agtaaagtaa taaggaggat agcctccacg atgccgtaca
    17701 tgcatttcct gctgataaaa ctgctcataa ttttgttttt tagccaattc tatggcgtaa
    17761 tgttgaggat tgaatgtttg gatcacgact tcgcctgctt tttccgctcg ccctgctcgt
    17821 cctgaaactt gggtaagcaa ttggaatgta tgctcactag aacgaaaatc cggtaaattc
    17881 agggaggtat cagcattcaa caccccaact aatgtcactt ctggaaagtc caaaccttta
    17941 gcaatcattt gtgtccctaa taatatatct gcttcttttg caccgaattt ttgtaaaatc
    18001 tgttcgtgtg ccccttttct tcgagtggta tctacatcca tacgcaaaat cctagcttgc
    18061 ggataaagtt ctcggagttc ctcttctact ttttgagtac ctgttccata gtagcggatt
    18121 ttattgccgc cacaattagg acagcgattg ggaataggtt cttcatgtcc gcaataatgg
    18181 cagcgcattg aacgtgtatc catatgcagt gtaagagaga tatcgcaatt aggacatgga
    18241 agaacaaatc cacagtccct acacatcaca aaagacgaat atcctcgacg attgagcagt
    18301 aggacagttt gttctttttt gtctaatcga ttttgtattt tttcctgcaa attagcagaa
    18361 aaagtagagg tccgatgatt ctgaaactct tcacgcatat ctacgatctc aatagctgga
    18421 agctgtgcag ctgctttggc tcggtggttt aactgcaata actgataaac gcctttttga
    18481 gctcgtgctc ttgattctaa agagggcgtc gcgcttccta gaactattgg gcaatgatga
    18541 tatttactgc gccaaattgc tagatctctc gcatgataac gcggtgtctc atcttgtttg
    18601 taacttgatt catgttcttc atcgataatg atcagcccga tatttttcag cggggcaaaa
    18661 atagcagatc gtgcgcctac tactacttgc gcttcttccc gctcaatctt gcgccattca
    18721 tcatattttt caccttggga aagtccgcta tgtaagaccg caactgattc tccgaagcgt
    18781 cctttgaatc gctcgaccat ttgcggagtc aatgcgattt caggcactaa cataatagcc
    18841 gttttatttt ctgacaaaac atcagcgatt gcctgaagat atacttctgt tttcccgctg
    18901 ccagtgattc cctccagcaa aaagacctga tctttttgct gctgacctgc tgtgatgatt
    18961 tgttcgacag catttttttg ttctgcattc agttccaatg ccgtcgtttg gtcaaatact
    19021 cggtctttat aagggtcgcg ataacgttct gattctataa actccagcca gcccttttct
    19081 tttccttgat tcaagacagc agtactgatt cctttatttt tgaaataagc aattggttct
    19141 tcttggctca gttcagataa acacaaaaga agttcttttt gtttcttggc attttgccgt
    19201 aattttgccc attcttcttc gatttgcttt tcagttaaca aagaacggac caaacgaatc
    19261 gtttttactt tatttcgtgt atggacttca tatttgatat ctactaattg ctcttggcgc
    19321 cagcgaatca attgagaaag gctgccttct tcttctgctt ccttccagga aatcacttct
    19381 cgctgatcaa acaatacctg aacttctggt gtgtctgcca aaggatagat caatttatca
    19441 tattctgctt tcatcacact cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa
    19501 gtaatttttt tcatataatc agctaatgaa agtaattctt cattgaccac cggagcaagg
    19561 tctagcaaac gaataactgc cttcaaagat ggattagctg attcttctga tttttggata
    19621 gccataacaa atccttggac atgacgattg ccattaccaa aagggacttc tactcgcatc
    19681 ccgacttgca aagctgtttc catttcagag ggaaccaaat aagtgaatgg ctgatcggtc
    19741 tgcatagtag gtacgtccac aatgacttca gctgttaatg tcatggttcg tttcctcctt
    19801 tttttgtctt ttcttatcaa gcgacaaaaa gttccttgcc tattttacta gagatcgatg
    19861 gatttgtctt atcttctgcc aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag
    19921 aactgtgtca aaaagttcag acacagcctt ttctaccaga caataagtgg cgaatgtttg
    19981 aaaaggaaac agctgggttt gcttttcttt ttagatgttc gctctatttt tctttattac
    20041 attttttgct cttcacggat acgtgattcc aattcgtgtt gttcacgctc tttttgtgct
    20101 tgacgttcct cttcttccat cttcaaacgt tcacgtttta attcaggatg tggatcattg
    20161 actacatttc ctgcatcgat ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa
    20221 gaatctagag taggctgtgc acttgcatcc aattcatgcg cgcgtttact tgctaaaatc
    20281 actagagaat attttgaatt tacacgatct aatagtgagt caattgaagg ttttaacatc
    20341 ataagactac atctccttta acattttaat atatttaccg attacgcgat ctactcgaaa
    20401 atgttcactg gcaataatgt ttttgattcg ttcaactgct agcggcactt catcatttac
    20461 aacagcatag tcgtaaagag ccatcatttc gatttcttct tttgcaactt tcattcgttt
    20521 ttcgattact tcatctgcat ctgttcctcg gcctacgatc cgtgatttta attccgctaa
    20581 atctggcgga gtcaaaaaga tgaatacacc atcgggcact ttttctttga cttgttttgc
    20641 tccttggact tcaatctcca agaaaacatc ttttccttca tccaatgtct tgttgacata
    20701 tgacaaagga gttccataat aattcccgac atattctgcg tattccagca tctctccagc
    20761 ttcgatcatt gattcaaact cttctttgct tcggaagaaa taatcaacgc cttcgacctc
    20821 cccttcacgc attttacggg tagtcattga gatcgaatac tgaaaatcat tatcctcact
    20881 ctcaaaaatt gcttttcgta ctgtgccttt tcctacaccg gatggaccag acaacacgat
    20941 taataatcca cgctctgaca tgacgacgtc ctttcaacta gtctttttgt acttatcgtt
    21001 tattttgcac tttttttctg caactttcaa ggttatcttt aattatagct agaaattggt
    21061 aaaaaagcta ccctgtacca agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa
    21121 cagccaaaca taaacagctt ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc
    21181 aagcttttga acgccgtgta taagaggtcg tgaagaagcc gattagtttc gagtatgaag
    21241 aaaaattttg tgaaaatagt ttctcacatt tttgcataaa tttggcttaa taccgagaaa
    21301 atggtttttg aacatcactt attcaaaata aaaaaagatt gtgacacgaa ttttgtcaca
    21361 acctttaaat gtattcctag agcaaagaag ctaagcgaag aaatccaatt ctgtttattt
    21421 attgtatttc tttgttcagc atccttgcta aaagcccata ttggacaaag tattcactac
    21481 acccatgatc tcatcttttc gagaacgtgc tttttctgtc ggatcttcta cctcttctgt
    21541 gtcaattcga acaataaatt tccgattcat cgtcacgtaa gcaccgtcgt tgatcaaaat
    21601 aaacagttcg ttctcattat agtctgcaca ggcatctttc catacgtcaa agccgttttt
    21661 ttcagaaggg atatcccgtt taaccacagt ttttccactt aattcgtgga acgttacgtc
    21721 ataatatttc attatctcta cctctttcgt cattcaatat tttgtacttg ttctctgatt
    21781 ttttctaaga tcgtcttaga ctggacgact tgatttttaa tttcgattgc atttgatttg
    21841 gaaccgatcg tgttgatttc ccgattcatt tcttggatca ggaaatctaa ttcccgacct
    21901 acaggacatt gtgcttcgat aagttcatct agtttttgta catggatcgc cagacgatcc
    21961 agttcctcgt gaatatctcc gcgttccaac aagatggcta gctccgtcaa caaccgttgc
    22021 tcatcgactt tcgctccaag aaattcctcc agcttttttt gataccttat ttgaaattcc
    22081 ttttcataga tttctacaaa actatcgagt ttttttagta cttctttgaa ttcttcagta
    22141 ttctttttca acactaaagc taaagcttga ccttcttttt ctcgactcgc ctcaatagaa
    22201 tcaagtgcgc ttttcaatgt atctagagct aactgtgcca attcttccat gctctcttcc
    22261 ggtttttctt ctaccgttac gaagttttcg tccagcacga agcgttccgc catccgacta
    22321 attgacactt gattttgact accgtatcgt tgttgaattc cgtctgtcaa ttgagtgata
    22381 agtgaatcaa tcaattccca gttgatgacg atctgttttt cgtttttgcc aatataagct
    22441 agattcacaa atacttcgat tcgaccgcgg cttaactgct gtttaataag ctgacgaaaa
    22501 tcgttttcaa caaagttcag gattttagga cttcgtattt gaacatctag aaaacgctgg
    22561 ttgacacttt ttatttcaat atctaactga tacgcttttg tttcgcggat ggctttgcca
    22621 aaaccagtca tacttttcat tcgacagggt ccttcctcat tacatatttt ctattgcttt
    22681 ttgcagttgt tcaaactctt cgttcgtcaa tgtcaggcct ttccccattt tttcatggtc
    22741 tggcgcccaa tctcgaagat cgaattttgg cgggcgacca ttccagctga ctaggttcag
    22801 ttctttacgc caccccttgt tgttttctga caatatagcg atttcttcta cgatttcata
    22861 tgaaaattct tgtgccatgg ttgttcctcc ttgagataat ataaatatag gatcagtaat
    22921 gtgtcaactg gttgcttaag caattcatgc tgcaaacgat ccttgcgttt tccttgtagc
    22981 agataatcaa aataaacagt tttcgtgcgc tcattttgta ttttttgctg gtattttcga
    23041 taccttcgcc attcttcagt acttaattga ccaatttgtg ccaataaatc atccggcata
    23101 ctttttatat cttgttggca gatcatttga atcatcatgt ctacttgata gatttcattg
    23161 ggcagtaaaa aatgtttttt tgctaaaagt tctagcatcg ccaatccgca tgtctctggt
    23221 acgaccctgt tcttatataa attacgcagt ttttgccaaa attttatgct tttgaaaaaa
    23281 gataattcta actgaatttc ttttgttaag taagacaaaa atcctcgcca gtatgttctt
    23341 gcaggtttaa cagaatgaaa tgtataccag ttcccatagt aatagcctaa tcgttttttc
    23401 atttccagat agcctaattg taaatttaat tggttccttt ggctatcaaa aagcagaaag
    23461 ctgcctagtg tccacaatgt ttgacatttc caatggataa aaatgtctgg catacgaatt
    23521 tttttcgctg ggcctggccc ttgcacatct acgacaaatg cctctgttgc tcctttgtta
    23581 atcgcaatat caataggtat tttgttgcga tacccaccgt ctgcatattt attttttccg
    23641 atctttcgat aagccatagc gggataaaat gaagcagagg cgagtatcca atctggtatt
    23701 tcttctgttt ttgtctgttg gatatcagtc actacttccg ctagttctgg cagtttagta
    23761 gacaccgtat ataaacgtat tttgggatta tgtttgagta aggacaaatc caaattgtct
    23821 ttgaccatct tttccaatgg agcagaagaa accccgcctt caattagtgc tgttttcgtc
    23881 atttggcgtc tttctcgata gaagcgttca cgaaggtctt caactgcagc catttctggt
    23941 aacgcaagca cttgtcttgt cgacagcttt ttccataaat tcacagcttt tttcatatct
    24001 ccttgtaaaa tcaatgcgcc gttcaacgca cctaccgagg tacctgtgat aatctcaaag
    24061 ttgatatcat gttccttcag cgcctgccac acaccaatct gataagcacc gtgcgctcct
    24121 cctccaccta aaacaagtgc tgtatgatag accagctttt tatttaattg tgtggttcct
    24181 tgatagccgt ttttatgaaa aatacgcaac cattcttctg atagaggaga ttgaaataaa
    24241 aaacaacagt tttctttaat aaaaaaacga gcgcaagctt ccatataccg aaaaaatttt
    24301 ttccattcaa aagtttcacc ttcaatcaac agattattaa aaataacatc tcctctactt
    24361 ttagcaatag taaaatagac ggtatatttt ttcgatactg ctttaaaagc acattgcttt
    24421 tttgcagaac gccaaaaagc gtctctactt tcttgatgac tttgaaaaaa tggaaggggt
    24481 tgttttgctt gctggatagg cgaatacagc gacattttct gtgaaaagta aacttttttt
    24541 atctccatcg cgctctcctt tttcttcaag tataccagta cgtcataaaa aggtcatgag
    24601 tctcgtattc gactttgtag attccataag aaaacaattc ataaaacctc ctttaaataa
    24661 aaaaatacaa aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat
    24721 tttgtaatgg aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata
    24781 aaagagatta agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat
    24841 tgataccaca agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag
    24901 tatcgtgcat tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata
    24961 tgaatcccat acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc
    25021 ctcttctcgt aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac
    25081 gatgctgaaa gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa
    25141 aacacatctt cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct
    25201 taatgatcag ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc
    25261 tagtaaatta agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa
    25321 agaaacacct gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca
    25381 ggaagggatc attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc
    25441 aatctatgtg attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga
    25501 aatcatcgct actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc
    25561 cgatctttct tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac
    25621 aagaaactag gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg
    25681 tcgtttatct ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat
    25741 cgttacaata gctgtactca acccgctggc accattcatt acgaaagaaa agagccaagg
    25801 attcatcccc catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa
    25861 atatctggaa agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt
    25921 attatcgtct acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa
    25981 agtataggca atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc
    26041 caacggaaaa tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct
    26101 tcttaacgca aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat
    26161 ctggatcgga atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca
    26221 aactctattt tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga
    26281 agcagctttc gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg
    26341 atacaggtac gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt
    26401 gagtttattt attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg
    26461 atacgtttga ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt
    26521 ttacgatctt ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct
    26581 tctattcttt gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta
    26641 gttgcaccag cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc
    26701 atatcatcga tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct
    26761 gcaacatttg cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc
    26821 gctaatttac gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg
    26881 cctttgattc ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg
    26941 actggaatat caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt
    27001 gttgcaccag ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca
    27061 cgtgctttac ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt
    27121 gcacttgctc gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact
    27181 gggctgctcg ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt
    27241 acttgaattt ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact
    27301 gctgctgcaa ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt
    27361 gggtcaaagt aatgttttga catggggacc tccactttct ttttcacaac tctagaattc
    27421 tacctaataa atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag
    27481 tttagataag ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt
    27541 gccagcgcat attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt
    27601 ttagctacct cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg
    27661 acagtcgtat gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca
    27721 ttgatttctt ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct
    27781 ttgccgattt gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt
    27841 tcagcttttg gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg
    27901 ctttcaatga ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt
    27961 gaatgtgacc cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct
    28021 gcttcgatca atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa
    28081 tcgacaaagc ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca
    28141 tttgctttag ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt
    28201 tgataagcag caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag
    28261 tattcacctt gagcattgtt tgttccaact ttgcttaacg catcgaacaa agcttcgttg
    28321 tcaaaacagt aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct
    28381 ttttgttcca cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct
    28441 gttgggtttt ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat
    28501 tcaaaaagat tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac
    28561 gttgttcctt tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc
    28621 aattgttctg cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct
    28681 gctccgtgtc caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg
    28741 atatgttcta ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc
    28801 atgcgggtac ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact
    28861 cctaaatata gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa
    28921 gtgattttct tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct
    28981 atctatttgg taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata
    29041 aaacatgaaa agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt
    29101 acaacctctt cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg
    29161 caaaatatga ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt
    29221 tcacaacctc tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
    29281 gaaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt
    29341 tttcacaacc tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg
    29401 agtatctaca tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt
    29461 gaattttgat tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa
    29521 gctcttcatt ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg
    29581 tgaaccacgt ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc
    29641 agacacatag ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat
    29701 aacgaatggc gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc
    29761 tacagtcatt acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac
    29821 ttgacgcagc agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag
    29881 cagacggtct tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc
    29941 atatgaaatt tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt
    30001 tccataccct cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga
    30061 tttagcagat tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt
    30121 caataaatac tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa
    30181 ctccttttaa gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca
    30241 ttttatgaaa aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa
    30301 aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg
    30361 caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga
    30421 ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta
    30481 gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac
    30541 cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca
    30601 tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta
    30661 gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata
    30721 aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg
    30781 taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc
    30841 tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga
    30901 tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa
    30961 cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg
    31021 ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca
    31081 cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga
    31141 attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc
    31201 cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac
    31261 atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt
    31321 tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat
    31381 tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc
    31441 tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca
    31501 gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc
    31561 attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt
    31621 tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac
    31681 caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac
    31741 atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat
    31801 ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag
    31861 ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag
    31921 tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct
    31981 cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca
    32041 caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg
    32101 aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag
    32161 tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt
    32221 caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt
    32281 tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc
    32341 aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt
    32401 ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt
    32461 tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca
    32521 cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg
    32581 gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg
    32641 taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt
    32701 ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca
    32761 aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat
    32821 taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg
    32881 atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag
    32941 ccatttcact acaataaaaa actgaagaat accattttag gattcctcag tttttcatca
    33001 cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa
    33061 ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg
    33121 gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg
    33181 atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt
    33241 ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga
    33301 tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt
    33361 ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct
    33421 ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc
    33481 aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct
    33541 cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc
    33601 ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag caataaagca
    33661 gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg
    33721 attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc
    33781 accatttcca actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt
    33841 atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc
    33901 gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt
    33961 ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt
    34021 gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt
    34081 cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca
    34141 aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag
    34201 gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa
    34261 aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc
    34321 attttctcgt atacaataaa cagattggct aacagatagt cagctaattc ctcagattct
    34381 ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg
    34441 atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga taatacatat
    34501 gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt
    34561 gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg
    34621 tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct
    34681 gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga
    34741 agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct
    34801 gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct
    34861 cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg
    34921 tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg
    34981 cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc
    35041 gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa
    35101 ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt
    35161 attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa
    35221 aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga
    35281 taaaattctt ttctggataa ccacacagct aacagaatca agacatatac caatatcaac
    35341 tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta
    35401 ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag
    35461 ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga
    35521 aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc
    35581 gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga
    35641 atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc
    35701 gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca
    35761 tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt
    35821 ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc
    35881 gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct
    35941 cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg
    36001 aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta
    36061 ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca
    36121 tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt
    36181 gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga
    36241 cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca
    36301 gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc
    36361 gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc
    36421 aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca
    36481 ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt
    36541 tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca
    36601 ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt
    36661 aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa
    36721 tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc
    36781 tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag
    36841 tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag
    36901 taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat
    36961 tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc
    37021 ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt
    37081 acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa
    37141 tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag
    37201 cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc
    37261 cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc
    37321 caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct
    37381 ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac
    37441 tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat
    37501 caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt
    37561 tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt
    37621 tttattattt agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc
    37681 caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt
    37741 ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg
    37801 cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac
    37861 ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg
    37921 ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga
    37981 tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa
    38041 cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac
    38101 gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa
    38161 cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga
    38221 tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc
    38281 cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac
    38341 gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc
    38401 ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat
    38461 cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac
    38521 ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg
    38581 ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt
    38641 tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag
    38701 tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt
    38761 ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct
    38821 gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt
    38881 catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt
    38941 ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct
    39001 tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga
    39061 ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca
    39121 taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca
    39181 taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa
    39241 ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact
    39301 ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta
    39361 cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt
    39421 taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta
    39481 tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg
    39541 tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc
    39601 tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat
    39661 accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat
    39721 atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc
    39781 accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga
    39841 gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt
    39901 ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc
    39961 tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt
    40021 gatttctgga ttcgcaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc
    40081 gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg
    40141 tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa
    40201 agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc
    40261 gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac
    40321 acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt
    40381 tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact
    40441 ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac
    40501 atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa
    40561 tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc
    40621 tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat
    40681 ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat
    40741 tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc
    40801 attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc
    40861 tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc
    40921 agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc
    40981 catctgctcc acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat
    41041 ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc
    41101 agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc
    41161 tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt
    41221 atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc
    41281 taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac
    41341 ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt
    41401 tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag
    41461 tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg
    41521 tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa
    41581 agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga
    41641 ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt
    41701 tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt
    41761 tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt
    41821 ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata
    41881 ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac
    41941 gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg
    42001 ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag
    42061 ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa
    42121 cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct
    42181 tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac ttacattgat
    42241 cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc
    42301 agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta
    42361 ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa
    42421 gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat
    42481 tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg
    42541 ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta
    42601 gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa
    42661 gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat
    42721 tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta
    42781 agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt
    42841 gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga
    42901 gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt
    42961 cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca
    43021 ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt
    43081 aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat
    43141 ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt
    43201 cccttgataa acataggcat tgtctactag cacacctaac catacacgat tgacagcagt
    43261 aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga
    43321 atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac
    43381 tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc
    43441 ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca
    43501 agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg
    43561 aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt
    43621 acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc
    43681 aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa
    43741 tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa
    43801 ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag
    43861 gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt
    43921 ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg
    43981 acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc
    44041 ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg
    44101 actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc
    44161 ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta
    44221 atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc
    44281 atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt
    44341 tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag
    44401 tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt
    44461 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat
    44521 tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt
    44581 ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc
    44641 taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa
    44701 ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg
    44761 gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt
    44821 aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt
    44881 cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat
    44941 gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc
    45001 aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg
    45061 attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt
    45121 gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt
    45181 aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat
    45241 ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc
    45301 ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa
    45361 taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt
    45421 gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata
    45481 caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat
    45541 ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg
    45601 tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata
    45661 gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg
    45721 aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc
    45781 tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata
    45841 attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt
    45901 caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt
    45961 attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta
    46021 cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata
    46081 attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt
    46141 ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg
    46201 ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc
    46261 ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac
    46321 tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag
    46381 gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat
    46441 acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc
    46501 gttccttcac gatattcact tgaggttggg tataaaacag atctggataa actgtttccc
    46561 gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga
    46621 caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac
    46681 tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc
    46741 tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca
    46801 aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact
    46861 gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa
    46921 aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat
    46981 gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt
    47041 gcgcacttaa ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga
    47101 gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc
    47161 ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat
    47221 acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca
    47281 acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt
    47341 tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga
    47401 cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa
    47461 aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc
    47521 ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg
    47581 gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct
    47641 gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg
    47701 tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta
    47761 gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg
    47821 gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa
    47881 gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa
    47941 taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt
    48001 aacttgctac ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat
    48061 ctgagattgc cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga
    48121 tcacgattgg tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct
    48181 ttcttcctaa accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac
    48241 tctccgcttc ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa
    48301 acgtatgcca agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca
    48361 caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt
    48421 caaatttctt tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat
    48481 accgattcgc acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta
    48541 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
    48601 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
    48661 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
    48721 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
    48781 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttacca
    48841 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
    48901 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
    48961 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
    49021 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
    49081 tgcttctttt aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa
    49141 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
    49201 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt
    49261 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
    49321 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
    49381 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
    49441 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
    49501 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
    49561 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
    49621 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
    49681 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
    49741 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
    49801 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
    49861 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
    49921 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
    49981 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
    50041 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
    50101 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
    50161 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
    50221 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
    50281 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
    50341 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
    50401 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
    50461 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
    50521 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
    50581 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
    50641 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
    50701 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
    50761 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
    50821 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
    50881 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
    50941 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
    51001 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
    51061 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
    51121 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
    51181 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
    51241 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
    51301 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
    51361 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
    51421 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
    51481 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
    51541 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
    51601 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
    51661 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
    51721 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
    51781 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
    51841 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
    51901 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
    51961 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
    52021 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
    52081 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
    52141 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
    52201 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
    52261 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
    52321 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
    52381 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg
    52441 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
    52501 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
    52561 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
    52621 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
    52681 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
    52741 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
    52801 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
    52861 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
    52921 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
    52981 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
    53041 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
    53101 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
    53161 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
    53221 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
    53281 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
    53341 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
    53401 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
    53461 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
    53521 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
    53581 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
    53641 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
    53701 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
    53761 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
    53821 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
    53881 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
    53941 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
    54001 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
    54061 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
    54121 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
    54181 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
    54241 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
    54301 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
    54361 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
    54421 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
    54481 cataccatcg cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
    54541 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
    54601 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
    54661 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
    54721 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
    54781 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
    54841 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
    54901 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
    54961 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
    55021 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
    55081 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
    55141 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
    55201 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
    55261 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
    55321 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
    55381 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
    55441 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
    55501 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
    55561 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
    55621 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
    55681 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
    55741 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
    55801 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
    55861 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
    55921 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
    55981 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
    56041 tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
    56101 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
    56161 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
    56221 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
    56281 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
    56341 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
    56401 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
    56461 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
    56521 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
    56581 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
    56641 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
    56701 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
    56761 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
    56821 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
    56881 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
    56941 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
    57001 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
    57061 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
    57121 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
    57181 cggttttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
    57241 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
    57301 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
    57361 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
    57421 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
    57481 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
    57541 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
    57601 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
    57661 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
    57721 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
    57781 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
    57841 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
    57901 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
    57961 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
    58021 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
    58081 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
    58141 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
    58201 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
    58261 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
    58321 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
    58381 cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt
    58441 tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt
    58501 tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac
    58561 aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga
    58621 aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa
    58681 tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa
    58741 tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt
    58801 acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca
    58861 agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca
    58921 gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac
    58981 attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc
    59041 agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat
    59101 ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc
    59161 agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga
    59221 aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag
    59281 taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg
    59341 aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa
    59401 ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa
    59461 caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc
    59521 atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc
    59581 gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat
    59641 gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat
    59701 gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg
    59761 atcggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc
    59821 aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta
    59881 ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag
    59941 tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta
    60001 gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg
    60061 gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag
    60121 ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac
    60181 gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa
    60241 aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta
    60301 gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt
    60361 gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact
    60421 aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa
    60481 gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt
    60541 ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg
    60601 ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa
    60661 tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac
    60721 tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg
    60781 aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga
    60841 agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg
    60901 ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat
    60961 tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt
    61021 tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa
    61081 aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt
    61141 agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac
    61201 cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg
    61261 ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct
    61321 tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc
    61381 tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca
    61441 cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact
    61501 tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa atgaatttta
    61561 gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa
    61621 aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat
    61681 aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag
    61741 agttaaacga cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta
    61801 ttagctcaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt
    61861 aggataaaac gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt
    61921 attagctcaa aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc
    61981 aggacaaaac gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg
    62041 gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga
    62101 cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa
    62161 aataattacg gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat
    62221 aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag
    62281 ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg
    62341 gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta
    62401 acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac
    62461 aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa
    62521 gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa
    62581 aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa
    62641 agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta tgattgctta
    62701 acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa
    62761 gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac
    62821 gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa
    62881 gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg
    62941 ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa
    63001 tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca
    63061 atcctgtcat caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
    63121 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
    63181 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
    63241 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
    63301 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
    63361 catacatgat ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga
    63421 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
    63481 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
    63541 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
    63601 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
    63661 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
    63721 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
    63781 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
    63841 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
    63901 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
    63961 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
    64021 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
    64081 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
    64141 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
    64201 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
    64261 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
    64321 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
    64381 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
    64441 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
    64501 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
    64561 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
    64621 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
    64681 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
    64741 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
    64801 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
    64861 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
    64921 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
    64981 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
    65041 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
    65101 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
    65161 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
    65221 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
    65281 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
    65341 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
    65401 gtttagagga agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt
    65461 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
    65521 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
    65581 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
    65641 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
    65701 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
    65761 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
    65821 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
    65881 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
    65941 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
    66001 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
    66061 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
    66121 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
    66181 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
    66241 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
    66301 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
    66361 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
    66421 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
    66481 gtaactgaaa accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc
    66541 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
    66601 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
    66661 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
    66721 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
    66781 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc
    66841 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
    66901 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
    66961 agcgaccaca aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt
    67021 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
    67081 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
    67141 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
    67201 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
    67261 tttcacaatg cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
    67321 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
    67381 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
    67441 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
    67501 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
    67561 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
    67621 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
    67681 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
    67741 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
    67801 cagtagataa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
    67861 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
    67921 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
    67981 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
    68041 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
    68101 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
    68161 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
    68221 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
    68281 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
    68341 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
    68401 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
    68461 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
    68521 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
    68581 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
    68641 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
    68701 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
    68761 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
    68821 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
    68881 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
    68941 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
    69001 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
    69061 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
    69121 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
    69181 tttctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
    69241 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
    69301 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
    69361 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
    69421 gtttcttttt aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc
    69481 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg
    69541 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac
    69601 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt
    69661 taatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
    69721 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
    69781 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
    69841 aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
    69901 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
    69961 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
    70021 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
    70081 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
    70141 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
    70201 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
    70261 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
    70321 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
    70381 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
    70441 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
    70501 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
    70561 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
    70621 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
    70681 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
    70741 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
    70801 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg
    70861 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
    70921 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt
    70981 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
    71041 taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag
    71101 agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa
    71161 acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga
    71221 gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt
    71281 ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat
    71341 tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa
    71401 ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa
    71461 actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata
    71521 ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc
    71581 tttagcaaag caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc
    71641 ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt
    71701 atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac
    71761 ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat
    71821 cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct
    71881 ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt
    71941 tttttccttc gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta
    72001 aataacataa gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac
    72061 tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa
    72121 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
    72181 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
    72241 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
    72301 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
    72361 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
    72421 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
    72481 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
    72541 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
    72601 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
    72661 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
    72721 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
    72781 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
    72841 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
    72901 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
    72961 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
    73021 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
    73081 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
    73141 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
    73201 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
    73261 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
    73321 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
    73381 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
    73441 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
    73501 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
    73561 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
    73621 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
    73681 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
    73741 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
    73801 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
    73861 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
    73921 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag
    73981 cttttgcttc attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg
    74041 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
    74101 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
    74161 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
    74221 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
    74281 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
    74341 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
    74401 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
    74461 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
    74521 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
    74581 caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga
    74641 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
    74701 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
    74761 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
    74821 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
    74881 taaaaggcga accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat
    74941 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
    75001 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
    75061 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
    75121 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
    75181 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat
    75241 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac
    75301 ctctgataaa cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat
    75361 gtggaaagcc attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa
    75421 cctctttata cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa
    75481 tggttttccc aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat
    75541 atcatgttgg atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag
    75601 attttcgaca aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg
    75661 ctctaatgcc ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc
    75721 tggagagtca ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatac
    75781 ccaacctgtt tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc
    75841 aatgataggt aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc
    75901 tgaatcctta atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg
    75961 atcggcatct gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt
    76021 cactacattc ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg
    76081 atctattcct ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat
    76141 attaaatcca gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat
    76201 ttcattttgt ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt
    76261 tgctcctgtt actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt
    76321 tacagctgta catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag
    76381 caaaaaaaat gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc
    76441 atgtacttgc ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg
    76501 ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat
    76561 ccggtttgat ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc
    76621 agatttcttt actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg
    76681 atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa
    76741 gctacgctcg gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc
    76801 tgatacataa aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag
    76861 agagattttt ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata
    76921 aattctttta ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct
    76981 caggtcaaag cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg
    77041 gcattagttc tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc
    77101 tcaggtcaaa gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat
    77161 ccagatttct cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc
    77221 cccaacatag gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa
    77281 atcatttctt aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt
    77341 tgggaatggg atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata
    77401 gtacatcatt ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc
    77461 ttgtttcaat gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc
    77521 tgcagccaaa ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac
    77581 cgtaaaatct ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat
    77641 attaagttca tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg
    77701 gatattttta ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac
    77761 attactgaaa tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata
    77821 atctgaagaa ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg
    77881 aaatggccct ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa
    77941 atcagcgtac ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt
    78001 ttcttccaag cgggctccat caaatccata agaaacgttg attacttgat ttggatataa
    78061 atctcctaac ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg
    78121 tatttcttga ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata
    78181 aaactgttct ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa
    78241 aaatgtcaag cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata
    78301 tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa
    78361 cgccgtttct gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg
    78421 gtttttgata tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg
    78481 atattcagag attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg
    78541 ttcttcgatg taatcgatca attggttcag atctttaagc atatttatct tcctccttgc
    78601 ttgccttttt atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta
    78661 gtcagatgta gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg
    78721 gataattggt attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag
    78781 acacattcta agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta
    78841 ctacctactg tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt
    78901 ttctttgcga tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa
    78961 tcacttcatt tttgtcagat aaataaaaaa caaaaaaagt tccatcttta aaacacgcta
    79021 ttcttttact ttttctcaca ttctctaaga aaagaaaagg agaatgaatt tttgacaatt
    79081 cgagcaaaag tgattatcaa atatgtaaaa tcagctactt ttttcttaaa cgtttcttta
    79141 tactaaatat aaataagtgt ttcatgaaag gagaactaga tgaataattg gcttgagtat
    79201 tttcctgaaa acgttttgga acgcggttac agctatcatc tgcacggttt tgtccgtcat
    79261 ttgaactata caagcaagta cttgtccgca acagtttctg gcacggaaga ttataaggta
    79321 gtgatcactt gggatgaaaa gaccaacatg acttgtgatt gtctatatgc catagaagga
    79381 aaaaaatgca aacacatggc tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa
    79441 tccaactact cactttccga actctcgtcc cttgtttcct ccgctagttc ctctcttgta
    79501 cgggagcttt taacagagat tcttatagaa catccgcaat ttatcgaacg cttcaaagtt
    79561 aagatgccat ttcatgcaat aaattattca gataaattaa caactatcat ccataaatac
    79621 gatcatatta tcaagaaaaa caaaaacagg aaaacagcga agttcatcat ggagatgcga
    79681 aagttcatcc aagaagcagt tgaatcgctc attcagcaaa atgcctatct tcccgcattc
    79741 gaattgatca atgaagtgat tgctactctc gaaacattct attgggagcc tgaggatgaa
    79801 cgaacccttc ttctgataga ggactgttat tatttatgga aagaattatt agcagaagca
    79861 cctcatgcag aaaaaaggca aatgttctct tggttcgtct gccaagtcga tcatactgac
    79921 gcctcttatt caaaacgcta ctccataaaa atcctgaaag aagactttcg ggaaaaagaa
    79981 ttttctaacc aaaagaaaaa aatagataaa aaaactaaag aaacggtaaa gaaagacgat
    80041 ttcaatgaaa aatagcaagt aaacgataac aaccatccta aatccacaag aaatacagca
    80101 gaccttgctt taaaaacacg aatcatgcaa aggatgaagt aaagaagact taacgcgtac
    80161 ccaatattag tcatttgact attaggtacg cgttttgttt tataagtcat gacaaatttc
    80221 cttcgttgct gggtagattt tccggcattg ttgatagatt ttatgatact tttctacatt
    80281 ttgtaagaga ggttccactg catctgtata ttggataaaa ttctgacaac aactttcagg
    80341 tgtgtcgaac cactctaatc tttaaagata aatattaaaa taacgattaa taatattaac
    80401 agtctttatt tttcaccacc aaaaacctat ctttgcatat actaaataga cgtatataca
    80461 cagataggtt tgtcttgtga aaaataatga ttatccttac caaaaaatgt ttgtttttct
    80521 acatgttcgt acttatcaaa aaatatgatc cttattgaaa ttactggcta tttatattcc
    80581 aacgacttct tgtgttaatt tttgttatct catagtaaat tatgtttcat tttcacttaa
    80641 tttagttgtc acgtgttcgt caattcgtaa aaacggtaaa tatataaaca cacttgcggc
    80701 tataattaat atttgtaaaa cagctgctcg ccagtctccg gctgttgcta aaaatcccga
    80761 tataactggt ggcgtagtcc aaggaatttg cactactaca tgattaatta atcccacagc
    80821 agttgcaaaa tatgctactg tcaaagaaaa aatcggtgca agcacaaagg gaataatcag
    80881 actgatatta aatacaatag gcaatccaaa aataataggt tcgcttatat tgaatatagc
    80941 gggagttacc gataatttag caaagtcctt ataatctttt ctacgactga agataaatat
    81001 tgcaatcaat aaagcaatcg tatttcccga tccacccatt accgcaaagg tatctttaaa
    81061 ggccatattg ataatatgtg gaatttgctc tttgtttgca taagctgcca tattttcttg
    81121 catattctga atcaaaaacg gatctaacaa tgaacctgat attactgctt gatggatacc
    81181 aaatccaaaa aatagattag caatagatgt aattaacaaa aatcctggta aaccagtt
//
LOCUS       DASJTM010000002        76955 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJTM010000002 DASJTM010000000
VERSION     DASJTM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.002"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..329)
                     /locus_tag="U4B29_000078"
     CDS             complement(<1..329)
                     /locus_tag="U4B29_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105058.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="U4B29_000079"
     CDS             complement(343..600)
                     /locus_tag="U4B29_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105059.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="U4B29_000080"
     CDS             complement(795..1337)
                     /locus_tag="U4B29_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105060.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="U4B29_000081"
     CDS             complement(1358..2449)
                     /locus_tag="U4B29_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2105061.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="U4B29_000082"
     CDS             complement(2457..4667)
                     /locus_tag="U4B29_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105062.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="U4B29_000083"
     CDS             complement(4683..7211)
                     /locus_tag="U4B29_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2105063.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="U4B29_000084"
     CDS             complement(7263..7673)
                     /locus_tag="U4B29_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105064.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="U4B29_000085"
     CDS             complement(7686..7904)
                     /locus_tag="U4B29_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105065.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="U4B29_000086"
     CDS             complement(7901..8893)
                     /locus_tag="U4B29_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2105066.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="U4B29_000087"
     CDS             complement(8972..9883)
                     /locus_tag="U4B29_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2105067.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="U4B29_000088"
     CDS             complement(9902..10246)
                     /locus_tag="U4B29_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105068.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10822)
                     /locus_tag="U4B29_000089"
                     /pseudo
     CDS             complement(10266..10822)
                     /locus_tag="U4B29_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10903..11403)
                     /locus_tag="U4B29_000090"
     CDS             complement(10903..11403)
                     /locus_tag="U4B29_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105069.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="U4B29_000091"
     CDS             complement(11404..11949)
                     /locus_tag="U4B29_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105070.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="U4B29_000092"
     CDS             complement(11946..13202)
                     /locus_tag="U4B29_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2105071.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="U4B29_000093"
     CDS             complement(13563..13874)
                     /locus_tag="U4B29_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105072.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15207)
                     /locus_tag="U4B29_000094"
     CDS             complement(13897..15207)
                     /locus_tag="U4B29_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2105073.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="U4B29_000095"
     CDS             complement(15821..16273)
                     /locus_tag="U4B29_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105074.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="U4B29_000096"
     CDS             complement(16275..16640)
                     /locus_tag="U4B29_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105075.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="U4B29_000097"
     CDS             complement(16739..21022)
                     /locus_tag="U4B29_000097"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN2105076.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="U4B29_000098"
     CDS             complement(21169..21408)
                     /locus_tag="U4B29_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105077.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21770)
                     /locus_tag="U4B29_000099"
     CDS             complement(21498..21770)
                     /locus_tag="U4B29_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105078.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21853..22104)
                     /locus_tag="U4B29_000100"
     CDS             complement(21853..22104)
                     /locus_tag="U4B29_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105079.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22267..23226
                     /locus_tag="U4B29_000101"
     CDS             22267..23226
                     /locus_tag="U4B29_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2105080.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23396..24076
                     /locus_tag="U4B29_000102"
     CDS             23396..24076
                     /locus_tag="U4B29_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2105081.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24166..25200
                     /locus_tag="U4B29_000103"
     CDS             24166..25200
                     /locus_tag="U4B29_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2105082.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25214..25549
                     /locus_tag="U4B29_000104"
     CDS             25214..25549
                     /locus_tag="U4B29_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105083.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25533..28013
                     /locus_tag="U4B29_000105"
     CDS             25533..28013
                     /locus_tag="U4B29_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105084.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28069..29628
                     /locus_tag="U4B29_000106"
     CDS             28069..29628
                     /locus_tag="U4B29_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105085.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29625..30989
                     /locus_tag="U4B29_000107"
     CDS             29625..30989
                     /locus_tag="U4B29_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2105086.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            31160..32230
                     /locus_tag="U4B29_000108"
     CDS             31160..32230
                     /locus_tag="U4B29_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105087.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            32245..32913
                     /locus_tag="U4B29_000109"
     CDS             32245..32913
                     /locus_tag="U4B29_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105088.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            32933..33511
                     /locus_tag="U4B29_000110"
     CDS             32933..33511
                     /locus_tag="U4B29_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2105089.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33912..34823
                     /locus_tag="U4B29_000111"
     CDS             33912..34823
                     /locus_tag="U4B29_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2105090.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(35201..36118)
                     /locus_tag="U4B29_000112"
     CDS             complement(35201..36118)
                     /locus_tag="U4B29_000112"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN2105091.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            36320..36757
                     /gene="zapA"
                     /locus_tag="U4B29_000113"
     CDS             36320..36757
                     /gene="zapA"
                     /locus_tag="U4B29_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN2105092.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            36815..37363
                     /locus_tag="U4B29_000114"
     CDS             36815..37363
                     /locus_tag="U4B29_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN2105093.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            37478..39838
                     /locus_tag="U4B29_000115"
     CDS             37478..39838
                     /locus_tag="U4B29_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN2105094.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39925..40239
                     /gene="trxA"
                     /locus_tag="U4B29_000116"
     CDS             39925..40239
                     /gene="trxA"
                     /locus_tag="U4B29_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN2105095.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            40421..42199
                     /gene="uvrC"
                     /locus_tag="U4B29_000117"
     CDS             40421..42199
                     /gene="uvrC"
                     /locus_tag="U4B29_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN2105096.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            42344..43243
                     /locus_tag="U4B29_000118"
     CDS             42344..43243
                     /locus_tag="U4B29_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105097.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            43236..44498
                     /locus_tag="U4B29_000119"
     CDS             43236..44498
                     /locus_tag="U4B29_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105098.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            44659..46557
                     /locus_tag="U4B29_000120"
     CDS             44659..46557
                     /locus_tag="U4B29_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN2105099.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46887..47606)
                     /locus_tag="U4B29_000121"
     CDS             complement(46887..47606)
                     /locus_tag="U4B29_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN2105100.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            47943..48275
                     /locus_tag="U4B29_000122"
     CDS             47943..48275
                     /locus_tag="U4B29_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105101.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            48262..50265
                     /locus_tag="U4B29_000123"
     CDS             48262..50265
                     /locus_tag="U4B29_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN2105102.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            50284..50754
                     /locus_tag="U4B29_000124"
     CDS             50284..50754
                     /locus_tag="U4B29_000124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN2105103.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            50775..51359
                     /locus_tag="U4B29_000125"
     CDS             50775..51359
                     /locus_tag="U4B29_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN2105104.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            51374..52360
                     /locus_tag="U4B29_000126"
     CDS             51374..52360
                     /locus_tag="U4B29_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN2105105.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            52350..52661
                     /locus_tag="U4B29_000127"
     CDS             52350..52661
                     /locus_tag="U4B29_000127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN2105106.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            52683..54464
                     /locus_tag="U4B29_000128"
     CDS             52683..54464
                     /locus_tag="U4B29_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN2105107.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            54457..55833
                     /locus_tag="U4B29_000129"
     CDS             54457..55833
                     /locus_tag="U4B29_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN2105108.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            55840..56475
                     /locus_tag="U4B29_000130"
     CDS             55840..56475
                     /locus_tag="U4B29_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN2105109.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            56505..57860
                     /locus_tag="U4B29_000131"
     CDS             56505..57860
                     /locus_tag="U4B29_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN2105110.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(58052..59446)
                     /locus_tag="U4B29_000132"
     CDS             complement(58052..59446)
                     /locus_tag="U4B29_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2105111.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            59538..60509
                     /locus_tag="U4B29_000133"
     CDS             59538..60509
                     /locus_tag="U4B29_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN2105112.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            60506..61288
                     /locus_tag="U4B29_000134"
     CDS             60506..61288
                     /locus_tag="U4B29_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN2105113.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(61457..61828)
                     /locus_tag="U4B29_000135"
     CDS             complement(61457..61828)
                     /locus_tag="U4B29_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2105114.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61962..62405
                     /locus_tag="U4B29_000136"
     CDS             61962..62405
                     /locus_tag="U4B29_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN2105115.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(62448..63257)
                     /gene="yidA"
                     /locus_tag="U4B29_000137"
     CDS             complement(62448..63257)
                     /gene="yidA"
                     /locus_tag="U4B29_000137"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN2105116.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(63276..64643)
                     /locus_tag="U4B29_000138"
     CDS             complement(63276..64643)
                     /locus_tag="U4B29_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2105117.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(64627..65457)
                     /locus_tag="U4B29_000139"
     CDS             complement(64627..65457)
                     /locus_tag="U4B29_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN2105118.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            65620..66057
                     /locus_tag="U4B29_000140"
     CDS             65620..66057
                     /locus_tag="U4B29_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN2105119.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            66133..66753
                     /locus_tag="U4B29_000141"
     CDS             66133..66753
                     /locus_tag="U4B29_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN2105120.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66942..67448
                     /locus_tag="U4B29_000142"
     CDS             66942..67448
                     /locus_tag="U4B29_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN2105121.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            67465..68364
                     /locus_tag="U4B29_000143"
     CDS             67465..68364
                     /locus_tag="U4B29_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN2105122.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            68451..69335
                     /locus_tag="U4B29_000144"
     CDS             68451..69335
                     /locus_tag="U4B29_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2105123.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(69527..69598)
                     /locus_tag="U4B29_000145"
     tRNA            complement(69527..69598)
                     /locus_tag="U4B29_000145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69563..69565),aa:Arg,seq:ccg)
     gene            69658..70992
                     /gene="rpoN"
                     /locus_tag="U4B29_000146"
     CDS             69658..70992
                     /gene="rpoN"
                     /locus_tag="U4B29_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN2105124.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            71141..72232
                     /locus_tag="U4B29_000147"
     CDS             71141..72232
                     /locus_tag="U4B29_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN2105125.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            72273..73274
                     /gene="gap"
                     /locus_tag="U4B29_000148"
     CDS             72273..73274
                     /gene="gap"
                     /locus_tag="U4B29_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2105126.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            73384..74574
                     /locus_tag="U4B29_000149"
     CDS             73384..74574
                     /locus_tag="U4B29_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN2105127.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            74678..75433
                     /locus_tag="U4B29_000150"
     CDS             74678..75433
                     /locus_tag="U4B29_000150"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN2105128.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            75549..76847
                     /gene="eno"
                     /locus_tag="U4B29_000151"
     CDS             75549..76847
                     /gene="eno"
                     /locus_tag="U4B29_000151"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN2105129.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        24692 a        14351 c        14858 g        23054 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa atcgccgatt gtaaaattaa gctagacaac
    22201 taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga
    22261 gtacaaatga cctataacca tcttacacca actgaactcg taatgataga agcttatttc
    22321 aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt
    22381 tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat
    22441 aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa
    22501 tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag
    22561 tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac
    22621 actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga
    22681 aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat
    22741 gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc
    22801 acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct
    22861 aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa
    22921 tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac
    22981 gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat
    23041 tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct
    23101 gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac
    23161 tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt gtctaactta
    23221 atttgacaaa cgaaacttaa taaaaaagga agaatattca tgtaaacgat tttattgttt
    23281 ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt catgaggatt
    23341 ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat ttattatgaa
    23401 aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct tggcaacacc
    23461 agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg aaccaacaaa
    23521 taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag cagatccgga
    23581 aaatccagca actggccaga caggtccttt aacactggat gtagttcccg aattgccatt
    23641 tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga
    23701 tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg
    23761 gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc
    23821 tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat ccactggacc
    23881 aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa tatttacagc
    23941 agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa atatcacact
    24001 gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga acctcgtggc
    24061 tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa tactataggt
    24121 gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa
    24181 cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc gtcaatcagc
    24241 caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga gaaccaaata
    24301 gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca gacactagaa
    24361 gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt caatccggcg
    24421 gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt agatgagaca
    24481 ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca
    24541 catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt taagggacgt
    24601 gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa
    24661 ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca agaaagtcaa
    24721 gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt caacgcaaag
    24781 ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa tttgatattt
    24841 acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa tgcattttta
    24901 gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa agcaaaagcc
    24961 ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca
    25021 caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc cgtttttgca
    25081 gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt tctagtgtta
    25141 tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga aacaacatga
    25201 tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa gtttgctgtt
    25261 cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga ttacatttta
    25321 tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac
    25381 acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga cgaatgaaca
    25441 aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg cgtggttgtt
    25501 gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa ttttcatttt
    25561 ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt tcaccttcgg
    25621 cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg tatgaaaata
    25681 tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc caactaactg
    25741 gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca aatgatcctt
    25801 ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca aatgtacttg
    25861 atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat ttgccggaac
    25921 atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac ttgtatattg
    25981 ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc cataccttgc
    26041 gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt gctattatta
    26101 cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg
    26161 cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt ttgaccaagg
    26221 gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt tatgattcga
    26281 ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca ggtagatttt
    26341 atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct gcaagtgcag
    26401 agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa tataatacat
    26461 ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc tctaccaata
    26521 gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca tttagtcgct
    26581 ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca atactgactc
    26641 atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac agtgggaaag
    26701 atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag tacaagctag
    26761 acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag ctcctttctt
    26821 ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag ggttcgatcc
    26881 attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca gatcaacttt
    26941 tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca ctacctgcat
    27001 tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt actaacatgt
    27061 atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact cttcctcaaa
    27121 taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac cttagcagac
    27181 aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga
    27241 cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg atctcacttc
    27301 ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact atcgcagata
    27361 ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata ggagatacca
    27421 ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa tccgtcagca
    27481 tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt gctaagcttc
    27541 ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa gaattaacat
    27601 tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg ttgaagacaa
    27661 gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg cttccaagca
    27721 cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat caaggtcaag
    27781 aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg attcagcaag
    27841 gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt ctcgttgact
    27901 ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct caacctggcg
    27961 caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc taagtgatta
    28021 gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat gagaaggaaa
    28081 cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt cagtttgctt
    28141 gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga aaaaggagag
    28201 gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt gaaagaagat
    28261 ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt tatccaagaa
    28321 gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta ttatcattgt
    28381 tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa tggctcattt
    28441 gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc tcgcagagtg
    28501 aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg gcggctgacc
    28561 ggtccagaaa gtcaaattag ttatttttat ttccaattat tttggtttgg acgtccctat
    28621 cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga tctgctgcaa
    28681 aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat atggctttcc
    28741 attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag caatcttcct
    28801 gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt gacccgttac
    28861 ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta tcttttcatg
    28921 atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt gataagcgaa
    28981 aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc gatagaagaa
    29041 tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt gacagtgatg
    29101 caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg ccaagaagga
    29161 ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg tgaaagattg
    29221 gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa tgcccgcttt
    29281 gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt agaattacgt
    29341 ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt gaaagaaatc
    29401 atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga gaaagaaaag
    29461 cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa gtccgctaaa
    29521 gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa ccaattttta
    29581 aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa atcgtgatca
    29641 ttggtgcttc ccatgaaggc atcacagctg ccctaaatct aagaaaactt caaccgaaag
    29701 cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt tcaaatggaa
    29761 tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat aacatgcgga
    29821 ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa cttgatccag
    29881 acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata acctatgata
    29941 aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt gaagctgaaa
    30001 atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag
    30061 caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca gatgctttga
    30121 gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta tcaagatatt
    30181 tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa ataaattttt
    30241 atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta tccgtacaac
    30301 ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact cgtcctaata
    30361 cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata gtcaatgaat
    30421 atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca gtcagttttg
    30481 acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat atggcaagag
    30541 cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa aagataacag
    30601 cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat gaagcgccat
    30661 tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca caatacgacg
    30721 aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta gaaataattg
    30781 gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt gcggatatcg
    30841 tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt ctgtctgaat
    30901 acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct aagagagcaa
    30961 atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt gtgtggggat
    31021 ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc ggaagaattt
    31081 tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat aaaatgatta
    31141 tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt caaagttacg
    31201 ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt ttttgactgg
    31261 gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg aggcagattc
    31321 gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta ctaagaagat
    31381 agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg gagtcgtgac
    31441 tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta tcgataaaaa
    31501 tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg atgaggcagt
    31561 aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag ttaagttatc
    31621 tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat tcaatgtctc
    31681 accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa aagaagatac
    31741 aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta atttacctga
    31801 agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat ataatgcgca
    31861 agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg
    31921 gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca
    31981 agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca ttttatcatt
    32041 tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc tacctgatgc
    32101 ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga tgttagtagt
    32161 agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc
    32221 aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt agaaaaaagt
    32281 ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag tatcgataaa
    32341 ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt tttgactttg
    32401 gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca agaattcagt
    32461 aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt tattttgcaa
    32521 gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt agataagatt
    32581 aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg cgtggataag
    32641 ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt cgcaatcgct
    32701 cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc tagtttggat
    32761 tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa
    32821 gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag aatatttatg
    32881 atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca
    32941 tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc
    33001 caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga tcacccagcc
    33061 caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt tagaaaacca
    33121 cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa cgggatttca
    33181 agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg atttgtttct
    33241 gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc ttttctttct
    33301 atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc aatctgtgct
    33361 aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt tagcacctta
    33421 catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat tgatcgattt
    33481 atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa aatagttagg
    33541 tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag gtagtttttc
    33601 ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg
    33661 gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga
    33721 aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc
    33781 aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc
    33841 cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt
    33901 gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta
    33961 tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta
    34021 cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa
    34081 tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg
    34141 caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga
    34201 tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa
    34261 agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc
    34321 atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa
    34381 cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt
    34441 tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa
    34501 aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa
    34561 ctggaatcac gacatattat tactatggac aagaggaaac attagcacag acaattgctg
    34621 actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc
    34681 ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg
    34741 atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag
    34801 cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat
    34861 ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt ttaaggaaga
    34921 ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa acagcttctc
    34981 atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac cgtttaagaa
    35041 aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga aaaatagttc
    35101 ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac accgtttatt
    35161 cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt agtagcttca
    35221 aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata ggcatgccgc
    35281 ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct gacggaaggg
    35341 tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca cgactgatga
    35401 tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag tacagttttt
    35461 cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc ggcgtaaact
    35521 gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc aaatggatcg
    35581 cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg atctcattgt
    35641 atttttctgg agataagaca agcagtttat aggggatggt tttttttaag atgtcagcaa
    35701 gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt ttacgtaatt
    35761 ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg aaatagctgc
    35821 catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt ggcaaagaag
    35881 aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta tcagcttcta
    35941 ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta atggtcgtcc
    36001 cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa taagagcgat
    36061 agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg ttgctcataa
    36121 aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc gttttctaag
    36181 agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt atgtgaaaaa
    36241 tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa ctttcatcaa
    36301 ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg tgattgccaa
    36361 ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg taacgaagct
    36421 gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata atgaacaagc
    36481 agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag aaagaatatt
    36541 ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta cagagcttga
    36601 aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa
    36661 tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt taaacgagaa
    36721 acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca aaataagaaa
    36781 aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt
    36841 attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc
    36901 gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca
    36961 tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag cattttttga
    37021 tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat
    37081 tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta
    37141 tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc
    37201 ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca
    37261 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
    37321 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
    37381 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
    37441 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
    37501 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
    37561 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
    37621 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaaat
    37681 tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga
    37741 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
    37801 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
    37861 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
    37921 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
    37981 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
    38041 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
    38101 tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg aaccaaaaca
    38161 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
    38221 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
    38281 tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
    38341 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
    38401 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
    38461 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
    38521 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
    38581 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
    38641 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
    38701 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
    38761 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
    38821 ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga
    38881 gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
    38941 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
    39001 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
    39061 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
    39121 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
    39181 ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa
    39241 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
    39301 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
    39361 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
    39421 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
    39481 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
    39541 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
    39601 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
    39661 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
    39721 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
    39781 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
    39841 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag
    39901 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
    39961 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
    40021 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
    40081 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
    40141 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
    40201 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
    40261 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
    40321 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
    40381 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
    40441 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
    40501 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
    40561 tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
    40621 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
    40681 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
    40741 aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg
    40801 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
    40861 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
    40921 atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
    40981 agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
    41041 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
    41101 tgccattgag acaatcatga cttgccaaaa aatgacaaat actgatttac tggatcgaga
    41161 tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
    41221 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
    41281 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
    41341 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    41401 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt
    41461 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    41521 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    41581 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    41641 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    41701 atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt
    41761 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    41821 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg ataaacataa
    41881 aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc
    41941 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    42001 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg
    42061 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    42121 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    42181 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    42241 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    42301 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    42361 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    42421 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    42481 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    42541 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    42601 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    42661 tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    42721 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    42781 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    42841 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    42901 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    42961 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    43021 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    43081 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    43141 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    43201 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    43261 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    43321 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    43381 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    43441 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    43501 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    43561 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    43621 gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct
    43681 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    43741 cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct
    43801 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    43861 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    43921 aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    43981 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    44041 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    44101 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    44161 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga
    44221 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    44281 tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg ggtggagcat
    44341 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    44401 tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat
    44461 cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg
    44521 aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat ttattttact
    44581 atattggata tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca
    44641 atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca
    44701 acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga
    44761 acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt
    44821 atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa
    44881 acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat
    44941 tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt
    45001 acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat
    45061 atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa
    45121 aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca
    45181 cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga
    45241 ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa
    45301 tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg
    45361 acacttttac ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg
    45421 ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt
    45481 gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat
    45541 caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa
    45601 tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat
    45661 ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat
    45721 ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag
    45781 cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc
    45841 acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc
    45901 ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa
    45961 cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt
    46021 tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt
    46081 gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac
    46141 ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa
    46201 gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc
    46261 aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg
    46321 acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat
    46381 tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa
    46441 aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa
    46501 ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa
    46561 gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct
    46621 ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa
    46681 caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct
    46741 cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    46801 tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct
    46861 ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc
    46921 tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta
    46981 tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa agcgatggct
    47041 tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga
    47101 tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat
    47161 tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct
    47221 tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa
    47281 attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga
    47341 atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct
    47401 tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga
    47461 gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct
    47521 tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt
    47581 ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa
    47641 ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
    47701 ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt
    47761 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
    47821 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
    47881 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
    47941 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa
    48001 agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat
    48061 tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac
    48121 aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
    48181 tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca
    48241 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
    48301 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc
    48361 cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc
    48421 acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa
    48481 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
    48541 cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat
    48601 aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat
    48661 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
    48721 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
    48781 agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg
    48841 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa
    48901 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
    48961 aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg
    49021 acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca
    49081 cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc
    49141 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa
    49201 accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag
    49261 caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact
    49321 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt
    49381 tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt
    49441 gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa
    49501 ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc
    49561 ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg
    49621 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg
    49681 gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc
    49741 agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc
    49801 atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc
    49861 ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa
    49921 ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt
    49981 ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct
    50041 ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
    50101 ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc
    50161 gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg
    50221 gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat
    50281 ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg
    50341 gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag
    50401 gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa
    50461 ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac
    50521 ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt
    50581 gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa
    50641 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
    50701 acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa
    50761 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag
    50821 agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac
    50881 aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
    50941 tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt
    51001 tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa
    51061 attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga
    51121 caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt
    51181 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
    51241 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
    51301 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
    51361 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
    51421 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
    51481 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
    51541 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
    51601 ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt
    51661 gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
    51721 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag
    51781 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
    51841 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
    51901 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
    51961 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
    52021 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
    52081 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
    52141 aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
    52201 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
    52261 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga
    52321 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
    52381 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
    52441 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
    52501 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
    52561 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
    52621 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
    52681 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
    52741 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
    52801 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
    52861 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
    52921 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
    52981 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
    53041 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
    53101 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
    53161 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
    53221 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
    53281 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
    53341 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
    53401 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
    53461 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
    53521 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
    53581 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
    53641 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
    53701 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
    53761 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
    53821 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
    53881 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
    53941 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
    54001 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
    54061 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
    54121 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
    54181 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
    54241 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
    54301 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
    54361 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
    54421 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
    54481 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
    54541 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
    54601 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    54661 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    54721 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    54781 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    54841 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    54901 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    54961 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    55021 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    55081 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    55141 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    55201 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    55261 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca
    55321 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    55381 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    55441 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    55501 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    55561 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    55621 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    55681 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    55741 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    55801 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    55861 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    55921 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    55981 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    56041 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    56101 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    56161 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    56221 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    56281 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    56341 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    56401 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    56461 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    56521 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    56581 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    56641 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    56701 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    56761 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    56821 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    56881 tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg
    56941 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    57001 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    57061 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    57121 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    57181 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    57241 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    57301 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    57361 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    57421 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    57481 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    57541 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    57601 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    57661 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    57721 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    57781 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    57841 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    57901 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    57961 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    58021 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    58081 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    58141 cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    58201 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    58261 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    58321 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    58381 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    58441 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    58501 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    58561 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    58621 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    58681 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    58741 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    58801 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    58861 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    58921 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    58981 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    59041 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    59101 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    59161 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    59221 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    59281 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    59341 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    59401 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    59461 aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt taatcttttg
    59521 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    59581 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    59641 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    59701 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    59761 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    59821 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    59881 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    59941 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    60001 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    60061 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    60121 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    60181 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    60241 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    60301 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    60361 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    60421 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    60481 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    60541 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    60601 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    60661 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    60721 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    60781 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    60841 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    60901 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    60961 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    61021 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    61081 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    61141 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    61201 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    61261 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    61321 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    61381 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    61441 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    61501 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag
    61561 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    61621 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    61681 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    61741 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    61801 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    61861 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    61921 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    61981 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    62041 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    62101 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    62161 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    62221 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    62281 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    62341 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    62401 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    62461 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    62521 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    62581 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    62641 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    62701 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    62761 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    62821 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    62881 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    62941 tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg tgtcagtccg
    63001 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    63061 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    63121 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    63181 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    63241 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    63301 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    63361 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    63421 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    63481 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    63541 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    63601 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    63661 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    63721 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    63781 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    63841 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    63901 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    63961 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    64021 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    64081 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    64141 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    64201 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    64261 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    64321 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    64381 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    64441 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    64501 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    64561 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    64621 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    64681 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    64741 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    64801 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    64861 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    64921 gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    64981 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    65041 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    65101 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    65161 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    65221 acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact
    65281 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    65341 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    65401 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    65461 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    65521 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    65581 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    65641 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    65701 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    65761 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    65821 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    65881 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    65941 ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg
    66001 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    66061 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    66121 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    66181 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    66241 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    66301 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    66361 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    66421 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    66481 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    66541 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    66601 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    66661 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    66721 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    66781 cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt
    66841 tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc
    66901 accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata
    66961 agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt
    67021 atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg
    67081 tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc
    67141 attttagtgc atggttttat cggatattga tccgcacggc attagatagt tggcgcagac
    67201 aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag
    67261 aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga
    67321 ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata
    67381 tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc
    67441 tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag
    67501 aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt
    67561 attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc
    67621 ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt
    67681 cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa
    67741 cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat
    67801 gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca
    67861 gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg
    67921 ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc
    67981 caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt
    68041 gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa
    68101 gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact
    68161 gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt
    68221 ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct
    68281 ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca
    68341 tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca
    68401 agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa
    68461 attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg
    68521 tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat
    68581 ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca
    68641 aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag
    68701 acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg
    68761 gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg
    68821 ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag
    68881 ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt
    68941 ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg
    69001 gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga
    69061 tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc
    69121 ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc
    69181 atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc
    69241 aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact
    69301 attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata
    69361 acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc
    69421 tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa
    69481 aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga
    69541 atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa
    69601 gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg
    69661 aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa
    69721 cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt
    69781 gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat
    69841 tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt
    69901 cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga
    69961 gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt
    70021 ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc
    70081 gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa
    70141 gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg
    70201 ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt
    70261 tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc
    70321 cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta
    70381 gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc
    70441 tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat
    70501 ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat
    70561 acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag
    70621 gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa
    70681 tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa
    70741 ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt
    70801 tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag
    70861 ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga
    70921 cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt
    70981 tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag
    71041 ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt
    71101 gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac
    71161 attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga
    71221 atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat
    71281 ctattggatg cagccgattg gacgtcgaag tttatcggaa agtatgggga tcacagaacg
    71341 agtattgcga acagaaacag atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag
    71401 cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga
    71461 tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg
    71521 ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga
    71581 agcgctgagc gaagccctag accgactact tccagaaggc gagaatatca tcgctgtgat
    71641 gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg
    71701 gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa
    71761 ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac tttatgttcc
    71821 ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt
    71881 cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat
    71941 ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt
    72001 tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag
    72061 aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg
    72121 aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct
    72181 catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata
    72241 tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt
    72301 ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag
    72361 tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa
    72421 ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa
    72481 aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg
    72541 tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt
    72601 taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa
    72661 tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt
    72721 catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg
    72781 ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac
    72841 ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact
    72901 caacaggtgc tgctaaagct atcggtttag taattcctga attgaacggt aaattagacg
    72961 gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg
    73021 aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt
    73081 acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat
    73141 tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg
    73201 ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact
    73261 tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa
    73321 gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat
    73381 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
    73441 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
    73501 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
    73561 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
    73621 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
    73681 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
    73741 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
    73801 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
    73861 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
    73921 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
    73981 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
    74041 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
    74101 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
    74161 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
    74221 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
    74281 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
    74341 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
    74401 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc tgcagcagca
    74461 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggtgg cgcaagcttg
    74521 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
    74581 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
    74641 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
    74701 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
    74761 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
    74821 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
    74881 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
    74941 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
    75001 catcaataaa aaagcaaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg
    75061 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
    75121 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
    75181 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
    75241 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
    75301 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
    75361 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcatttttgt cattgttaga
    75421 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
    75481 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
    75541 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
    75601 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
    75661 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
    75721 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
    75781 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
    75841 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
    75901 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
    75961 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
    76021 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
    76081 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
    76141 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
    76201 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
    76261 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
    76321 tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
    76381 tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg
    76441 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
    76501 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
    76561 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
    76621 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
    76681 tctctgatat cgctgttgca acaaatgctg gccaaatcaa aactggttct ctatcacgta
    76741 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
    76801 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
    76861 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
    76921 ctctctactt gctgaaatat cagcaggtag agagt
//
LOCUS       DASJTM010000003        61965 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJTM010000003 DASJTM010000000
VERSION     DASJTM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.003"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..672
                     /locus_tag="U4B29_000152"
     CDS             154..672
                     /locus_tag="U4B29_000152"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2105130.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            800..1483
                     /locus_tag="U4B29_000153"
     CDS             800..1483
                     /locus_tag="U4B29_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN2105131.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1792..1865
                     /locus_tag="U4B29_000154"
     tRNA            1792..1865
                     /locus_tag="U4B29_000154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Arg,seq:tct)
     gene            2024..2383
                     /locus_tag="U4B29_000155"
     CDS             2024..2383
                     /locus_tag="U4B29_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN2105132.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2506..4137
                     /locus_tag="U4B29_000156"
     CDS             2506..4137
                     /locus_tag="U4B29_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN2105133.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4199..4840)
                     /locus_tag="U4B29_000157"
     CDS             complement(4199..4840)
                     /locus_tag="U4B29_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2105134.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5026..5102
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5122..5430
                     /gene="rplU"
                     /locus_tag="U4B29_000158"
     CDS             5122..5430
                     /gene="rplU"
                     /locus_tag="U4B29_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN2105135.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5457..5801
                     /locus_tag="U4B29_000159"
     CDS             5457..5801
                     /locus_tag="U4B29_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN2105136.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5814..6107
                     /gene="rpmA"
                     /locus_tag="U4B29_000160"
     CDS             5814..6107
                     /gene="rpmA"
                     /locus_tag="U4B29_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN2105137.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6541..9348
                     /locus_tag="U4B29_000161"
     CDS             6541..9348
                     /locus_tag="U4B29_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2105138.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9539..10030
                     /locus_tag="U4B29_000162"
     CDS             9539..10030
                     /locus_tag="U4B29_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2105139.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10133..11122
                     /gene="manX"
                     /locus_tag="U4B29_000163"
     CDS             10133..11122
                     /gene="manX"
                     /locus_tag="U4B29_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN2105140.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11155..11958
                     /locus_tag="U4B29_000164"
     CDS             11155..11958
                     /locus_tag="U4B29_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN2105141.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11979..12893
                     /locus_tag="U4B29_000165"
     CDS             11979..12893
                     /locus_tag="U4B29_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN2105142.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12976..13350
                     /locus_tag="U4B29_000166"
     CDS             12976..13350
                     /locus_tag="U4B29_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN2105143.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13489..13734
                     /locus_tag="U4B29_000167"
     CDS             13489..13734
                     /locus_tag="U4B29_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2105144.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13906..14970
                     /locus_tag="U4B29_000168"
     CDS             13906..14970
                     /locus_tag="U4B29_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2105145.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15216..15641
                     /locus_tag="U4B29_000169"
     CDS             15216..15641
                     /locus_tag="U4B29_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2105146.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15634..16083
                     /gene="nusB"
                     /locus_tag="U4B29_000170"
     CDS             15634..16083
                     /gene="nusB"
                     /locus_tag="U4B29_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN2105147.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16180..17034
                     /locus_tag="U4B29_000171"
     CDS             16180..17034
                     /locus_tag="U4B29_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN2105148.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17035..18372
                     /locus_tag="U4B29_000172"
     CDS             17035..18372
                     /locus_tag="U4B29_000172"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN2105149.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18377..18601
                     /locus_tag="U4B29_000173"
     CDS             18377..18601
                     /locus_tag="U4B29_000173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN2105150.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18603..19493
                     /locus_tag="U4B29_000174"
     CDS             18603..19493
                     /locus_tag="U4B29_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2105151.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19502..20320
                     /locus_tag="U4B29_000175"
     CDS             19502..20320
                     /locus_tag="U4B29_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN2105152.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20427..20876
                     /locus_tag="U4B29_000176"
     CDS             20427..20876
                     /locus_tag="U4B29_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2105153.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20894..22576
                     /gene="recN"
                     /locus_tag="U4B29_000177"
     CDS             20894..22576
                     /gene="recN"
                     /locus_tag="U4B29_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN2105154.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22631..22747)
                     /locus_tag="U4B29_000178"
     CDS             complement(22631..22747)
                     /locus_tag="U4B29_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN2105155.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22820..23776)
                     /locus_tag="U4B29_000179"
     CDS             complement(22820..23776)
                     /locus_tag="U4B29_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2105156.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23947..24315
                     /locus_tag="U4B29_000180"
     CDS             23947..24315
                     /locus_tag="U4B29_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN2105157.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24485..24916
                     /gene="mraZ"
                     /locus_tag="U4B29_000181"
     CDS             24485..24916
                     /gene="mraZ"
                     /locus_tag="U4B29_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN2105158.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24937..25896
                     /gene="rsmH"
                     /locus_tag="U4B29_000182"
     CDS             24937..25896
                     /gene="rsmH"
                     /locus_tag="U4B29_000182"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN2105159.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25901..26302
                     /gene="ftsL"
                     /locus_tag="U4B29_000183"
     CDS             25901..26302
                     /gene="ftsL"
                     /locus_tag="U4B29_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN2105160.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26303..28495
                     /locus_tag="U4B29_000184"
     CDS             26303..28495
                     /locus_tag="U4B29_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2105161.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28521..29483
                     /locus_tag="U4B29_000185"
     CDS             28521..29483
                     /locus_tag="U4B29_000185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN2105162.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29497..30858
                     /locus_tag="U4B29_000186"
     CDS             29497..30858
                     /locus_tag="U4B29_000186"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN2105163.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30884..31972
                     /gene="murG"
                     /locus_tag="U4B29_000187"
     CDS             30884..31972
                     /gene="murG"
                     /locus_tag="U4B29_000187"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN2105164.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31987..33207
                     /locus_tag="U4B29_000188"
     CDS             31987..33207
                     /locus_tag="U4B29_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN2105165.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33363..34688
                     /gene="ftsA"
                     /locus_tag="U4B29_000189"
     CDS             33363..34688
                     /gene="ftsA"
                     /locus_tag="U4B29_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN2105166.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34711..35952
                     /gene="ftsZ"
                     /locus_tag="U4B29_000190"
     CDS             34711..35952
                     /gene="ftsZ"
                     /locus_tag="U4B29_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN2105167.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35969..36646
                     /locus_tag="U4B29_000191"
     CDS             35969..36646
                     /locus_tag="U4B29_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN2105168.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36660..37259
                     /locus_tag="U4B29_000192"
     CDS             36660..37259
                     /locus_tag="U4B29_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN2105169.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37274..37561
                     /locus_tag="U4B29_000193"
     CDS             37274..37561
                     /locus_tag="U4B29_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN2105170.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37610..38392
                     /locus_tag="U4B29_000194"
     CDS             37610..38392
                     /locus_tag="U4B29_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN2105171.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38413..39120
                     /locus_tag="U4B29_000195"
     CDS             38413..39120
                     /locus_tag="U4B29_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN2105172.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39382..42180
                     /gene="ileS"
                     /locus_tag="U4B29_000196"
     CDS             39382..42180
                     /gene="ileS"
                     /locus_tag="U4B29_000196"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN2105173.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42229..43755)
                     /locus_tag="U4B29_000197"
     CDS             complement(42229..43755)
                     /locus_tag="U4B29_000197"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN2105174.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43770..44417)
                     /locus_tag="U4B29_000198"
     CDS             complement(43770..44417)
                     /locus_tag="U4B29_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN2105175.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44601..44930)
                     /locus_tag="U4B29_000199"
     CDS             complement(44601..44930)
                     /locus_tag="U4B29_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN2105176.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45107..45835
                     /gene="sfsA"
                     /locus_tag="U4B29_000200"
     CDS             45107..45835
                     /gene="sfsA"
                     /locus_tag="U4B29_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN2105177.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45851..46864
                     /locus_tag="U4B29_000201"
     CDS             45851..46864
                     /locus_tag="U4B29_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2105178.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46864..48141
                     /locus_tag="U4B29_000202"
     CDS             46864..48141
                     /locus_tag="U4B29_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2105179.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48204..50906
                     /locus_tag="U4B29_000203"
     CDS             48204..50906
                     /locus_tag="U4B29_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2105180.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51058..51375
                     /locus_tag="U4B29_000204"
     CDS             51058..51375
                     /locus_tag="U4B29_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2105181.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51405..51725
                     /locus_tag="U4B29_000205"
     CDS             51405..51725
                     /locus_tag="U4B29_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2105182.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51833..53293
                     /locus_tag="U4B29_000206"
     CDS             51833..53293
                     /locus_tag="U4B29_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2105183.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53361..53582
                     /locus_tag="U4B29_000207"
     CDS             53361..53582
                     /locus_tag="U4B29_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105184.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53613..53795
                     /locus_tag="U4B29_000208"
     CDS             53613..53795
                     /locus_tag="U4B29_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN2105185.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53795..54208
                     /locus_tag="U4B29_000209"
     CDS             53795..54208
                     /locus_tag="U4B29_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN2105186.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54331..55512
                     /locus_tag="U4B29_000210"
     CDS             54331..55512
                     /locus_tag="U4B29_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105187.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55583..55747)
                     /locus_tag="U4B29_000211"
     CDS             complement(55583..55747)
                     /locus_tag="U4B29_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105188.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55901..55974
                     /locus_tag="U4B29_000212"
     tRNA            55901..55974
                     /locus_tag="U4B29_000212"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55935..55937,aa:Arg,seq:cct)
     gene            complement(56043..57182)
                     /locus_tag="U4B29_000213"
     CDS             complement(56043..57182)
                     /locus_tag="U4B29_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2105189.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57481..58116)
                     /locus_tag="U4B29_000214"
     CDS             complement(57481..58116)
                     /locus_tag="U4B29_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105190.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLNKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58229..58864)
                     /locus_tag="U4B29_000215"
     CDS             complement(58229..58864)
                     /locus_tag="U4B29_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN2105191.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58898..59359)
                     /locus_tag="U4B29_000216"
     CDS             complement(58898..59359)
                     /locus_tag="U4B29_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN2105192.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59489..59920)
                     /locus_tag="U4B29_000217"
     CDS             complement(59489..59920)
                     /locus_tag="U4B29_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2105193.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59938..60258)
                     /locus_tag="U4B29_000218"
     CDS             complement(59938..60258)
                     /locus_tag="U4B29_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2105194.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60557..61333
                     /locus_tag="U4B29_000219"
     CDS             60557..61333
                     /locus_tag="U4B29_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN2105195.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61348..61551
                     /locus_tag="U4B29_000220"
     CDS             61348..61551
                     /locus_tag="U4B29_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105196.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61567..61905
                     /locus_tag="U4B29_000221"
     CDS             61567..61905
                     /locus_tag="U4B29_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN2105197.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        20441 a        10420 c        13003 g        18101 t
ORIGIN      
        1 caattattta taataaaaaa tgagataaag atgaggaaat agaattagaa acatataatt
       61 attaaaggga aacattttta cagctataat ttgaaaaaat ttatcgttat tatatgatta
      121 agaaggattg aataaagaaa gaagttgaaa acgatacctg attacaaaaa aataactttt
      181 ataagttcta taacgttgat tatattttta attcttattc ttctcgtcgt cttggttccc
      241 cgaatttttg gtttgactcc ttatattatt gaggacaaag aaatgagtcc atattatgca
      301 caaggaaatc tgatatatgt acgaccgaaa aatacaaatg agattttagt cggagatatt
      361 attacatatt atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa
      421 aacaaccacg aagattttta tacaaaagct gatacaaaga cttatattga gcctggagta
      481 gtaaaaaaga gaaatattat cggtagtccc atctttcaga ttccatatat tggactagtc
      541 ctttcaaaaa cggcgtttgc atggattcag gggttattct ttacgattgc tttttgccta
      601 acaggaatca cagcatggaa tacctttgta gagttgaaaa agaagcggag acgaacacag
      661 aaatttttgt aagaatgtta aaaacataaa tgtattccag agatccaacg actcttctga
      721 acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt tcgtataatg
      781 tgtgaagagg aggagtagta tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga
      841 actattggat tttatgcgta cccagcaaaa aaaattaccg ggtgagctag gcaaactgga
      901 agaagaagct catgtagcag aagtaccagt tattccccat gaaacagttg tatttttgaa
      961 atttttactt ggacagcttc agcctgaacg aatcttggag atcggagcag ctatcggttt
     1021 ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct catgttacga caattgatcg
     1081 tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa cgtctaggac tgacagataa
     1141 agtgactttg cttgaaggtc aagcagcaga tatcttgccg gagttgtctg gtccgtatga
     1201 ctttattttt atggatagtg caaaatcaaa atatattgaa tttttacctg aatgcttacg
     1261 tttgttaaga aaaggtggcg ttttgatggt agatgacatc ttccaaggag gaacgatctt
     1321 gctcccagat gaagagattc cccgtggcaa acgagctatc catcgtaaat taaatgaatt
     1381 tcttcgtgtt gtgatggatc atcccgattt gacatcgaca atcttgccat taggtgatgg
     1441 tgtgatctta atgacaaagg aatcagaaac aatcgagttg tgatacatga ttatggcagc
     1501 ctgtagataa tcgattgaat cgagggctca attcgaactt gatgtaaaag tttctatatt
     1561 tgtaagtcaa ttaaaggata caggaaatct aaataaacag gagtagcatg aagtcaatgt
     1621 agctcctgtt ttcaattgat aaccttagaa cagctgaagg agaatgtctc gttttaaaag
     1681 attgatttag tagttgggcc aaaataattc attatttgct aattaaataa ctatttcgta
     1741 ttgacagaag caatttaatt atctataatt attgatgttc cgctgattct tgtcatagta
     1801 gctcagctgg atagagcatc cgccttctaa gcggacggtc gggggttcga atccctcctg
     1861 tgacgctaaa aaaccgttag aaactgaggt ttctaacggt ttttctttgt tttttgtgcc
     1921 aatattgttt gttttcaaag gcaaataaaa taaaaaaaca tccttatcga gtacaattat
     1981 aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa gatatgacga tactatctag
     2041 aattttagtg actctagttg caattgaatt tttttatatc atgtatatcg aaacggtgag
     2101 aactgattct gacacgacaa gtagggtttt taaaatgagt aaagaagagt taagcagaaa
     2161 atctgttcaa acattgttta agaaccaagg agtgtacaat ggactattag gagtgggact
     2221 actttatgga gcttatcttt cttccgcatc taaagaaatc acaagtatgc tattaatatc
     2281 tatttttttt gttgcattat atggcagttt gactagcgat cgttggattt tactcaaaca
     2341 aggtgggcta gctctgttgg cattgctttc tattttctta taaatataga aaaaataaga
     2401 atttatctaa caggaaactt tgtgaaaaat attactttat ctcatgaaat atgatattct
     2461 aatgaatggt attttagaaa atttcatgaa aaaggagtta gataggtgtt caaaaattat
     2521 gtttcctcac taaaaaaaga attttcaggt tatgatcttt caagatttca gaaagatctt
     2581 ttggctggga tcacagtagc tgctgtagca ttgcctttag ccttggcttt tggggtatca
     2641 agcggagctg atgcggccgc aggtttgatt acggctatca ttgccggtat cgttattggc
     2701 ggtttatcag gcggttttta tcaaatatca ggtccgacag gtgcgatggc agctatcttg
     2761 atgtctattg cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt
     2821 ctatttttac ttgcagcagg acttttccga ttaggaacac tgacttcttt tatcccagca
     2881 cccgtaatta cggggtttac ttcaggtatt gctatcatta ttgcattagg tcagatcgat
     2941 aatttatttg gtgttcactc ggaaggtaca aacgttattg aaaaactaag tagttatcaa
     3001 acgataggat ttccgataaa cacagcctct ttgatcatgg gagggctagt cattttaggg
     3061 atgtttatct atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct ggctatcatt
     3121 ttggcaacag cagttatgct attgttccat ttaccagtag caactgtggg taagattcca
     3181 caaacattga tcagtagtaa tcgtttgaat atgggagatt ttagcttttc tgccttgcaa
     3241 caagtggcag ttccagctat tagcattgct ttattaggaa tgattgaaag cttgctatgt
     3301 ggtgcttcgg ctggccgaat ggcaaataag cccttagata gcaatcaaga attaatggcg
     3361 caaggaattg gaaatctgtt gcttcctttc tttggcggga ttcctgctac tgcggccatc
     3421 gcccgtacaa gcgtggccat caaatctggt gcacaaacta gattagctgg tatgatacat
     3481 gcagttgtct tactgctctc catgcttgtt tttgcgccag tgatgtcaca gattccaatg
     3541 ccagcacttg caggtgtttt gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc
     3601 aaggagctat ttactaataa atattggtca gcagttctat tgtttatcct tacgatgatt
     3661 tgtacagtta tctttgattt aagtattgct atcgtgatcg gggtgatcgg cggctgtgta
     3721 ttttttgttg tcaaaagcgc tgctatcacg atttcattag aaacgatcga ttggcaaaga
     3781 atggggctgc cagaaacgga aaagctgaat aactggacag tagtgtatat tagtggtcca
     3841 ctgtttttta tgtcttcaga aaaactcaaa aataaattgg aagaactaaa tcatcacgat
     3901 ggtgtcattt tctccatgcg aggcgtacca agtattgatt tgacggcacg tgcagttttt
     3961 gatgaatttc aagaaaaagc agagcaaaga ggacaaacga ttatctatac ttccttgcag
     4021 ccagaagtag aaaaacaact ttctcacttg tgggaacata cattcacgca tcaacatcaa
     4081 acagttgctc atgcgttgac agctttacat gcgaaatata ctgcaattag ccattaaaac
     4141 aatgaaaaag aacagacaag actgtaaact gaagatttta tggtcttgtc tgttcttttt
     4201 aatattgctg taaaagtgaa gctagtactt gccagtcttg atggataaca ggtacaaggg
     4261 agcgattgta gaaaatagct gctggatgaa aaagtggaag aattcgatag tttttttctg
     4321 accattcgta ggcatccttt tgttctgtta attgtaagac aggccctgta tagagttgac
     4381 catgattttt ggtaaccgta tattctttgc caagtaaacg ttgaagacca atgtttccaa
     4441 gtgtcgcaat taattttggc tggaccttca tcagttcgta atccaatatt ggtgcatgcg
     4501 caaatacttc ggaacgagta ggtgtccgat ttggataaac agtttctgac tgttgtgtcc
     4561 gtttattgat ccgatgagtc acgcgatacg ggcggctcct tacagcactg gtgatataca
     4621 cctcttctcg tgttaagcca gttgaagaaa gtgcctgcat caattctttt ccagcttgac
     4681 cgctaaaagg aacatgcgta tcaatctctg ttttccctgg ggcttctccc acgagcatca
     4741 gtttaggatg aagaggtcct tgcccagcga taaatccttc tacaggacgg ccgttcattc
     4801 ttaaagaagc ttcttgtatc agttcttttg ggtattccat ttatgctcct cctttttctt
     4861 ttattataag aataaaagaa acacaatgcg actattttat ttggtagaac tgtgaggata
     4921 gaaaagcaga agtattgctt gaatttttgt tttacttttt gattttaaat ttagttgact
     4981 aacggttgct gttctggtaa gctagtactg ttgtaattgt ggactccaca gctacaaccg
     5041 ctcagaacaa gtaataagca cagcatatgc tgtcacttcg gatggcgagt ctaagtatat
     5101 cagaaggagg tgctttatag catgtacgca attattaaaa ctggtggtaa acaagtaaaa
     5161 gttgaagtgg gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt
     5221 gtttttgacg aagttatctt agtaggcgga gaatctacaa aagtaggcgc tccaactgtt
     5281 gcaggtgcaa ctgttgaagg tactgttgaa aaacacggca aacaaaagaa agtcgttact
     5341 ttcaaataca aacctaaaaa acattcacac cgtaaacaag gtcatcgtca accatataca
     5401 aaagtggtta tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc ctatatatga
     5461 tcaaaggaac atttaagcga aatgacgcag gtcagatcgt ttcattcact ttgaccgggc
     5521 atgctgatgc tggtccttat ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga
     5581 tcagcactgt caacgggatt gcctcattag ccggttttga gccgattgtt gaaatgaatg
     5641 aagaagaagg cggttatctt tataccgaag tcacttcagg aatgacacaa gaacaaaaca
     5701 atattgctga gattctatta gaaaatcttt tattaggatt acaatcgata gaagctgaaa
     5761 actcagaata tatccaaatt aaaacaatta atgataaata ggaggtgcag accatgttgt
     5821 tatctatgaa tctacaatta tttgcccaca aaaaaggtgg cggttctaca tctaacggcc
     5881 gtgactcaga atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca gttacaggtg
     5941 gatcaattct ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg
     6001 gtggagacga cactttattt gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc
     6061 gtaacaaaaa acaagtgtct gtttatccag tagctcaaga agcttaattg aatcttatga
     6121 ttaaagaggt tgtgacaaga cttttgtcac agcctttttt gtttataaaa ataaaagttg
     6181 tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt gccaatttgg
     6241 aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg tttgctatca
     6301 gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa aaatcgtatc
     6361 gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat caacaaaaca
     6421 aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt ttgaagccta
     6481 agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga ccgaatttat
     6541 atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca aggagtaaca
     6601 acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa agatctgaat
     6661 cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg ttacatatat
     6721 caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta caaagaaacg
     6781 ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt tgcaaaaatc
     6841 attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc gattttgtat
     6901 ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa gacttatttt
     6961 gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga aacggaactg
     7021 attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag tcatttattt
     7081 gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat tatcgatcaa
     7141 gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga aggtcaagaa
     7201 atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac aacgaaatcc
     7261 catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc ttctctatta
     7321 gaaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga aaaacgtcct
     7381 gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag
     7441 cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt gacctatgga
     7501 aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt tctaaatcat
     7561 atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat tcgtagcgga
     7621 ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat attggagcca
     7681 aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc ctatgagtta
     7741 ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc ggatggtttg
     7801 gatcaagaag caatcaatca tttcatttct acagatatca acatccatct gaagtctttc
     7861 tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt tgtagatccg
     7921 aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt accatatgag
     7981 tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt gaaaaaaatc
     8041 aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt tgattttcca
     8101 gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt tcaagtggag
     8161 attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg ggcaaatcaa
     8221 gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc aagcagtatg
     8281 gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga catgcctttg
     8341 gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga agtagatgaa
     8401 ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt
     8461 caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc tctagttttg
     8521 gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca tgaatcattg
     8581 aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga agcaaaacct
     8641 attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac agcacaaaga
     8701 atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt agaagttcta
     8761 gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga atatcagatc
     8821 atagctacta ctgggattac tgatttgaat atccaagccc catttattcc tttagaacgt
     8881 tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc agaattggag
     8941 gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt tgagtttatc
     9001 tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg gcaattttct
     9061 acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa aatcaaccta
     9121 gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt gactgtggaa
     9181 gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc agcctcagtt
     9241 gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta
     9301 cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat acacaaaaac
     9361 gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta aattcaatta
     9421 gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt
     9481 gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag ttttgcagat
     9541 ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg caacagtttg
     9601 gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa
     9661 attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa atgtaataac
     9721 cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa aagtgatgct
     9781 tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc
     9841 gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca attttgtttc
     9901 agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag tggagttaga
     9961 gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag cgaaaatcaa
    10021 aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta atggttagca
    10081 attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa atatggtagg
    10141 gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg gttcaatgat
    10201 ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag gtccagatga
    10261 cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg aagtgttatt
    10321 cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat tcgaagagca
    10381 caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg aagcctacgc
    10441 atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg aaacagcaaa
    10501 agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga aagcagcagt
    10561 tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag acggaaaaat
    10621 caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg cgacagcttg
    10681 gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat caaaggatga
    10741 tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa atgttattcc
    10801 tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca aagcattgct
    10861 tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag acatcaaaga
    10921 attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta aagtattgtc
    10981 tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga
    11041 tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga aaaaagcaaa
    11101 agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct tatcatgtct
    11161 attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga aggtattctt
    11221 gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt agttaccggt
    11281 aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt aggttgggca
    11341 aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc agcaattatt
    11401 ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat tgccgttcct
    11461 ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt tccaatcgtt
    11521 catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat gtggcacatc
    11581 attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt gctatttatc
    11641 ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac agatggtatg
    11701 gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa catgatggct
    11761 acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat ttctcaatta
    11821 acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt gaacctttct
    11881 aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc tttaggcgac
    11941 attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat
    12001 tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa ggttcatgga
    12061 actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca atcaaaaaat
    12121 tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa ttctttaata
    12181 cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa gaagaacgtg
    12241 cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt atgatgggtc
    12301 ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg ctaggtgcac
    12361 taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc ttcttaggtt
    12421 ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac aaagctggtt
    12481 ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa
    12541 ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc aaattcttac
    12601 cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat aaattgcctt
    12661 caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta gccctttctc
    12721 ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt ttagctgcat
    12781 taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca atcgttatca
    12841 tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta taatccataa
    12901 aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc ataaacaaat
    12961 atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt aattgatgct
    13021 acttcgttta cgggtctgac agattacggg caaatcatga taggtaataa tggttttgag
    13081 ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga agtggattat
    13141 gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat tcaaacgaaa
    13201 aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga
    13261 gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt agtgaaacgt
    13321 ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa gaggaggttt
    13381 tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt atttgtcccc
    13441 tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat gagttggtta
    13501 tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga
    13561 aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag tattggacaa
    13621 gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc atctatttta
    13681 ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc ataagttttt
    13741 acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg gctttattta
    13801 tttaaaataa aaagaagttt tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt
    13861 ttgctataat ggaaaaaagc gtgcaaagaa agagggagtt tcattatgat gctacgtgta
    13921 gaaaaattgc gcaaaaaaat gcaagaagaa aatttggatt catttttggt taccagtccg
    13981 tacaatcttc gttacttaac gaactttaca ggtacgacag gattagctgt tattactttg
    14041 gaaaaagcat tttttattac tgattttcgt tataccgagc aggcggcagc gcaggctcaa
    14101 gggtttgaaa ttattaaaaa tgtcggacct atttttgatg aggtagcaaa cttagtacgc
    14161 aaagaagaac tgcactcatt agcttttgag gaaacaacag tctcattttt agaatattcg
    14221 gttttagaag aaatcatcga tgcggaatta gtacctgttt cacagatgat cgaagaactc
    14281 agagaagtga aagatgaaga agaaatcgct attatcgaaa aagcatgtca tattgcggat
    14341 atggcatatg atcacatcct gaaaatgatc caaccgggca tgactgagat tgaagtcgca
    14401 aatcaactag acttttatat gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc
    14461 gttgctagcg gattgcgttc ggctatgccg catggtgtag caagcaaaaa aatcatcgaa
    14521 caaggtgact tgatcacatt ggactttggt tgctattacg aagggtacgt ttccgatatg
    14581 acacgtacat tcgctattgg tgatcctggg gaaaaattaa aagaaattta tcaaatagtc
    14641 ttagatgctc aattagcagt tattgatgct gcaaaaccag gaatgactgg tgtccaatta
    14701 gatgcagttg ctagagatta tatttcaaaa catggttacg gggaagcatt tggacattct
    14761 acaggacatg ggatcggttt agagatccac gaaggaccaa acgtttcttc tcgtgcagaa
    14821 aagcaatttg taccaggaaa tgtgatcact gatgaaccag ggatctactt accaggaatc
    14881 ggtggagtac gtattgagga tgacttgttg atcacagaag atggaaatcg tgtgttgact
    14941 cgttcaccaa aagaattgat catcttatag tttcatcaat agaagaagta agcgtaaaaa
    15001 tcagtgttca cagactttgt tatagaaaat cactcgattt aatgatacac tatagaagaa
    15061 cttttaagct gtaagagata ataaagttgg gtgttttctc caattcatag cagtttgcag
    15121 ctgatcggcg gcagttttct taccacgtga tcactatgcc gcatcataga gaattcggat
    15181 gtatctcgtg cttaggcaaa gggaggaata aataaatggc agatgaaaaa aatttgattt
    15241 taaacgcaaa ccaagattta ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta
    15301 tcgggattgc agcttctaaa gtagaaggcg tatacggaat gcgcggaact tttgccagca
    15361 atgtcactga attattagga cgtgcagctc acggaaaagg tgtttactta gcaaacgagg
    15421 aagatggctt gaaagtagat ctttactgtt atctagaata tggtgtttca gttcctaaag
    15481 tagcaatgga aatgcaggaa cgggtcagcc agcaagtcct tttcatgacg gatatcgatt
    15541 tatctgaagt caatatccat gtagtagctg ttgttcctga aaaattagaa caaccaacaa
    15601 tcgaagaact ttttgaagac gaagaggaag agaatgaata aagaattatc aagacacgag
    15661 attcgggaaa tggcattgca ggctttattt ccattagact tcaatgcaga cctaacaaaa
    15721 gaagatgcga ttttcaacgc aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa
    15781 tcagagtttg ttcctgttta tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat
    15841 gaattggata aagtgattga aaaacattta aaaaacaatt ggcacatcaa tcggatctca
    15901 aaaatggatt tggtgatctt gcgtatcgcg atttttgaaa tgacttatgt gacagatgta
    15961 ccagcggctg ttgctttaaa tgaagcaatc gagttggcaa aaacatttag tgatgaccgt
    16021 tcacgtaaat tcgtcaacgg tgttttatcc aatgtattga aagaattaga agctggggcg
    16081 taagtcctag cttttttcta tgaatcatca taaacctgtt ataaatcggg aaaattctat
    16141 ggtagaatga aaacacaaaa aatatctggg agtggttaga tggcagaatt gattaacgga
    16201 aaagaattag ctgaaaaaat gcaggcagag attgctgaaa aaatccaaaa attgaaagat
    16261 aatggtatcc atcccggatt agtcgtttta ttggtagggg aaaatccagc cagccaaatc
    16321 tatgtaagaa ataaagaacg tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt
    16381 tatcctgaga cgatttctga agacgaacta cttgcagaaa ttgaaaaata caatcaagat
    16441 cctagttttc atggtatatt ggtccaatta ccattgccaa agcatataga tgaagaaaag
    16501 atcctgttag ctatccgccc tgaaaaagac gtagacggtt ttcatccgat gaatttaggc
    16561 agactccttg cgggtaaacc ggacaagatt ccttgtactc catacggcat catgaaaatg
    16621 tttgaagctt attctatcga tccggcgggt aaacgagcag tagttatcgg acgaagcaat
    16681 atcgtcggta aaccgatggc tcagctactc ttgatggcag atgcaactgt tactgttgct
    16741 cattctaaaa ccgaaaatct ggctgaactg gcacaagaag cagatatttt agtcgtagca
    16801 ataggacgtg gacattttgt cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat
    16861 gtagggatga atcgtgatgc taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg
    16921 gagcctattg caggctggat cactcctgtg ccaaagggtg tagggccaat gactatcacg
    16981 atgctgatgt atcaaaccgt aaaatctgcc gaagcttttg gagcaggtga atagatggca
    17041 caagaatatt taactgtcac tacattaacg aagtatttga aaagaaaatt tgatgccgat
    17101 ccttatttag aacgcgtata tttaacaggc gaaatatcga atttccgttt acgtgccaat
    17161 gcccatcaat atttcagctt aaaagatgac catgcaaaaa tatctgctat tatgttcaaa
    17221 ggagcattcc aaaaattacg attccagcca aaagaaggaa tgaaagtcct tgtcgttggg
    17281 cgtatttcat tatatgaagc gggaggttct tatcaaatct atgtagagca catggagcct
    17341 gatggaatag gtgctttata tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa
    17401 ggcctttttc aagggcctaa aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt
    17461 accagtccaa gcggagcggt tatccgcgat atcatcacga cggtgaaaag aagatatcca
    17521 attgtccaat tggttttgtt cccaacagtt gtacaaggaa accaagcagc ggacaatgtt
    17581 gtcaggaata tccagagagt agaagagatg ggcaatttcg atacaatgat catcggtcgt
    17641 gggggtggtt cgattgagga tttatggccg tttaatgaag agcgggttgc tcgggcgatt
    17701 tatcaagcta atacgccggt gatttcatct gtaggacatg aaacagatac cacgatcgct
    17761 gatttagtag cagatgttcg ggctgccact ccaacagcag ctgcagaatt agctgttcct
    17821 gttttgtcag aagagttgat gcgcattcaa gaacggcaag ctagattgca gcaggcgttt
    17881 gtacgccaga tccagcgcaa acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt
    17941 cggcagcctg aaagattgta tgaaggtcag accatcaaat tagaccagtt gaaacagcga
    18001 ttatatcaag ctacacagca aatttatcat gcgaaagaaa aacaaacgat cgcattaagc
    18061 caccaattgg aacaagtggc tccgctttat cgagttaaag cagctcgcca agaaactgct
    18121 tatctagaaa aacgcttaga agagaaaatc caacaatata tgaaacaaca gcagcagcgt
    18181 tttgctcaag ctgttcaatc tcttgatttg ttgagtccat tgaaaatcat gggaagaggc
    18241 tacagctata cgacgttaaa tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg
    18301 gatttgctga cgatccatta tcaagatgga atcgttaaag gacaagtaca agagatagac
    18361 aaaggagaat aaaaatatgg aaaatccaac ttttgaagag tcactgcaag aattagaaaa
    18421 aattgtcatg cagttagaac aaggagacgt tccattagaa tcagctctag attcgtttaa
    18481 acgaggaatg gaattaagca aacattgcca agatacatta gctaaagcag aagagacctt
    18541 gacgaaaatg atgacagaat caaatgaaga agttgatttt gacggaaacg aggaaagctg
    18601 agatgagatt aactgacttt tcagctattc atctacctgc tgtagaaaaa gaaatcttgt
    18661 cttttttaga tgagcataca acggatagag gactctatga tgcgatgagt tattcagtga
    18721 aagcaggcgg gaaaagaata cgtcccttat tacttttaac ggccgttgct tcctttgatg
    18781 agcctattga tgttcccgtc tatcaagtag cagctgcttt agaaatggtg catacgtatt
    18841 ccttgatcca cgatgatttg cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta
    18901 ccaatcataa agtattcggc gaagcattag ctatattggc cggagatggt ttgcttacag
    18961 gagcattcca gctaatcagt atggcacact taggtaattc tcctaagctt ctgctgcttc
    19021 agcaattagc tgtttgtgcg ggatcacaag gtatggttgc tgggcaagct gcagatatcg
    19081 agggcgaatc gaaaaagctt tctttagaag aattagcatt tattcatgaa agaaagacag
    19141 ggcatttgat tcgttatgcc ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa
    19201 tcttattgct acttcaacga ttagccgaac atttaggttt agcttttcag ataagagatg
    19261 atctactcga tgtgattggt acaacgaaaa cattagggaa aacagctggt aaagatgaac
    19321 gtatggaaaa aaatacttac ccgcgtttat taggcctaga aaagacacgt gaggcgttgg
    19381 agattgagct gttatccgcc aacaagatca tagacaaatt agaagaggac gttttttctt
    19441 ttgatggcgg gctgatgcgc cagatgatca agcaatttga tgtggagaaa tgagggaaag
    19501 aatggaaaaa gaacgcgtag atgtattagc ggtcaaacaa ggattatttg aaacaagaga
    19561 acaagccaaa cgttcagtga tggctggttt gatttacaac gaaaaaaacg agcggtttga
    19621 taaaccggga gaaaaaattc cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc
    19681 ttatgtttct cgagggggat taaaattaga aaaagcactc aaagcatttg atctatctgt
    19741 tgaaggaaaa acattgttag atattggtgc atccactggc ggatttactg atgcagcttt
    19801 gcaaaatggc gcaaaaatga gttatgcttt agacgttggc tacaatcagc tagcttggaa
    19861 aataagacaa gatcctagag tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc
    19921 cgaagacttt accttaggaa ttccagaagt tgctgttatc gatgtttctt ttatttcact
    19981 aaaactgatg ctgcctccat tacatgctat tttaaaagaa gagggagaag tgattgcttt
    20041 aatcaaaccc caattcgaag caggcagaga agctgtaggg aaaaacggaa ttgttcgtga
    20101 ccctcagacc catcaaaaag ttttagaaga cattttgtca tttgcggcac aaggtggata
    20161 tgatgtgctg gaattaagtt attctccgat cacgggggga gaaggaaata tcgagttctt
    20221 ggctcacttg aggaaagtac ccgaaagcgg aacaatcaat tcagctatta atatggcaga
    20281 agtggtttca aatgcacacg aacagtttga ccataaataa attgtaactg tgaaaatcaa
    20341 gtgagaagta tgcggattct tctttattat accaaggaaa taagctatca ttgattaaga
    20401 agaatatgct aaggagcgaa gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc
    20461 gattgttgag tgagcaagat atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca
    20521 aaggaatatc tgttactcaa gctacgattt ccagagatat aaaggaactg aaactgatca
    20581 aggtaccggc ttcagatggc ggctaccgtt atagcatacc agctgaaaca aacgaagatg
    20641 taaatactaa attggaaaaa ctattaaaag acgctttcgt ggcagttgac caaatggaaa
    20701 aatttgttat tttgaaaaca ttgccgggaa atgcttcggc agcagcaaat ttgattgata
    20761 aacgttacaa gaaagaatta ttttcgatta tcaatgatga cgataatgtt ttgatgatta
    20821 cacgaactga agaccaggca gttttattga agaaagactt cctgcattac ctttaagttg
    20881 aggagcgaat gtgatgctac aagaaatcag tattactaac tttgcgatta ttccggaatt
    20941 gcggctttct tttcacgaag gaatgaccgc gctgaccgga gagactggtg cgggtaaatc
    21001 cattattatc gatgctttag gtctgttagc aggaggaaga ggttcaagcg attatatacg
    21061 tcaaggagca gaaaaatgtg tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt
    21121 ctctgaactg atggttgaat tagggatcga aacagatgag gacaatctga ttgttcgacg
    21181 ggatatgtcc ttaactggaa aaaatgtttg tcgagtgaat ggtcatatta tcacattagc
    21241 taatctgaga agaatcggca gttatttagt agatatccaa ggacaaaacg aacatcagga
    21301 actgctccag cctgaatcac atcttgcttt attggatcgt tttggagatg tagctttcca
    21361 acagaagaaa aagagttatc agcaagaata tatctcttat cgtgagctgg aaaaaagggt
    21421 acgaaaagtg cagcaaaatg aaaaatccta tgttcagaga atagatatgc tccatttcca
    21481 gcaagaagaa atagctgctg ctgatttgca agtcggagaa gaagagaaat taaaagaaga
    21541 aagagaaaaa cttagcaatt atcaaaaaat tgctgatgga ttagctgcag gttacggagc
    21601 actgacgaat aatgaacaaa acagtgtaga tggtgttggc ttggccgttt cagaactgca
    21661 agggatcgcc catctagatg tggagtatga agcaatttat gaaaatatcc aaagtgctta
    21721 ctatttgctg caagatgcaa tcggcgatat gagtcgtcag attgatttat tggagttaga
    21781 tgagagtcgt ttagaagaag ttacgcaacg tttagaatta atccgacagt tgaaacgtaa
    21841 atacggcgaa tccattgagt caatattggc ttactatgac gaaatcaccg aagagctagc
    21901 ttcttctgat ttttcagaag gtcaattgga caaaatgaaa gaggaactag agcaaaaaga
    21961 gcttctactt cagcaacaag ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga
    22021 attggaaaaa tccattctgc atgaattgaa aagtctgtat atggaaaaca ctgagtttga
    22081 ggtacgtttc ttaaaagaag aaaatagaca attgaatcat gatggatttg accaaatcga
    22141 attttacatt acgaccaatc caggagagcc actgaagcct ttggtgaaag tcgcttctgg
    22201 tggcgaactg tctcggatgt tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt
    22261 gacaagtatt atttttgatg aagtggatac aggtgtgagc ggaagagttg cacaggccat
    22321 cgcagataaa atttctcaaa tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc
    22381 tcaagttgct gcagtcgccg actatcaata ttatattgtg aaagcagtga ttgatggacg
    22441 tacgcaaact tctgtttcag agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat
    22501 gctggctgga agtgagatta caaaactcac actcgaacat gcaaaagaac tattgaaact
    22561 cgcaaaaagg acctgagcca cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta
    22621 tattttattt ttacatgaca ctcattacag cagtcaatac aacagatcca atcgttaaaa
    22681 tcagcattgt ccaaatgaca aattttgtta ctttagcaaa agtacttgtt tgtttttttt
    22741 cacgcattgt tttcaacctc gtcattaatt agttgattca agtgtacaat cttttttcca
    22801 tttccacaag agggcttttt tataaaaact tccctttttt taaataatag gtagttacga
    22861 tacaaataaa taatgttaat agaactagcc atataaaggc atcatccctt tcagcaatag
    22921 gtaacttaat gttcattcca tagatactgc cgacgattgt cggtatagtt aaaataatcg
    22981 ttaaagaagt taaaattttc ataatattat tcagattatt cgatacgatt gctgaaaaag
    23041 tctgattcat ttgttctact agttttaatt gaatgcgaga agtagtcatt gcctgctctg
    23101 tttccaccaa tacatcacgt aaatgatttt tcaaagcatg attttcttgg aaaagtttat
    23161 tttgcgctag ttctttcaat gcatctaaat tagcagaagt cgcttctctg aaataaacga
    23221 ggcttttttg gatatccatg atttgataaa gttgtttgtt ttcagtggat acttgaagtt
    23281 tggcttccaa agtatcgcat tgagattgaa gcgtagctaa ataactgttg taagagagtg
    23341 tgacctgcca aatgaattga aagaagatat tgagattagg cgaaatatca ctttttggaa
    23401 aggtctggct aaagactttc tttaaaaaca gtggttcatg attcgtaatt gtgataagct
    23461 tctttttggg cgtcaaaata atagagagcg ggtatgtatc cacttgaaaa taaccgctag
    23521 gacttgtttt agcaaaagga tattgtaaca ataataacgc tggctttgtc aactttgatt
    23581 gattgaattc ttctgtacgg gaattttctg catcgtccaa tatggaagtg atgtaatctg
    23641 acggaatttc aaattgatgt gtcagctgat gaatctcttc ttgattagga cgttctacac
    23701 aataccagat cgtttggtca gtttcctttc cggtattacg cagtttctca ttatctaatg
    23761 taaaatattt gatcatgatt ttcctcctgt tgcctgtttc ttttattata acggataggg
    23821 aattggaata catggaatgt gtttgtaaga tatagaatat aactgcataa atttaaataa
    23881 aatgaaacag ttgtgtaaag tatatacagt atacttgaag caacgataaa aaaggagttt
    23941 acgttcatgg gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt
    24001 ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa
    24061 gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca
    24121 gggacaattt ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt
    24181 gctgttttcc aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact
    24241 tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata
    24301 ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca
    24361 gcttgatttt taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa
    24421 ggtggtagaa agtgggggat tgtggtagac taattttagt tagtggagga atggggggct
    24481 ttcaatgttg atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc
    24541 ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg
    24601 tcttttcggc tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc
    24661 gttggctaaa aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg
    24721 cgaacttgat aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt
    24781 gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg
    24841 atggcaagat ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat
    24901 tgatttcgga ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat
    24961 acagtcatgt tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta
    25021 gactgtacac tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa
    25081 gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta
    25141 gcttcttatg tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa
    25201 gaacgcttga gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc
    25261 tctcctcagc tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat
    25321 atgcgcatgg atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac
    25381 catgaattag taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct
    25441 cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa
    25501 atcattaaac aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga
    25561 atttttcaag ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta
    25621 gaacaggcaa tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca
    25681 ttggaagaca gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca
    25741 ccgggacttc ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa
    25801 ccaatccttc cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta
    25861 cgtattgcag aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat
    25921 gcaagattac gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga
    25981 ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat
    26041 ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt
    26101 tttaacagtt atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca
    26161 aacagaaatc aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact
    26221 ttctcgtact gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga
    26281 caatttaagg aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa
    26341 gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag
    26401 tattggattg ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt
    26461 tgctggtacc tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa
    26521 agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc
    26581 ttattcaatt aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt
    26641 tgaagaaaag aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa
    26701 aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt
    26761 tggtagctat gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa
    26821 aaaagaaggt gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg
    26881 cgtcttttct tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt
    26941 tggtaaacta ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat
    27001 tatttatcaa aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa
    27061 agctgtcgac ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga
    27121 aacactgatg gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat
    27181 gaaagcaaaa accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac
    27241 aatggaagga ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta
    27301 tgaaccagga tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt
    27361 caacgagaat gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga
    27421 ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa
    27481 cgtcgggatg gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa
    27541 actgggattt ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc
    27601 tacattaaat attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa
    27661 tttccaaatg atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg
    27721 atatatcagc aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt
    27781 tctagggcaa cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt
    27841 tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt
    27901 atcggcaaaa aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga
    27961 tacagcctac ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt
    28021 gtatctaaca atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa
    28081 tccattgatg aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac
    28141 aaaaacagaa aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc
    28201 tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa
    28261 acaatctact gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg
    28321 tggtgataag ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg
    28381 gaaaatcctc ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact
    28441 tgctccatat gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga
    28501 gaaataggag agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt
    28561 gtgcaatgac aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag
    28621 gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa
    28681 tgggcggctt agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc
    28741 aaaaacagct aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa
    28801 tcggctttct tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga
    28861 agcagaaatt gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg
    28921 aaggttatcc aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg
    28981 gtattttcgc tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca
    29041 tcgatggatt agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt
    29101 ggcatcagca tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg
    29161 gtttcttcgt ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag
    29221 cgttgggcgg acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt
    29281 tgattggttt gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta
    29341 agcttacagg aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg
    29401 gatggtcgga gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga
    29461 tcacattatg gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa
    29521 aataagaaag tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta
    29581 catgaattgg gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa
    29641 gcacaagagc tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg
    29701 ttggatgaag aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta
    29761 gtagcaaaag cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa
    29821 gtagcggaat gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg
    29881 atgacaggac tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat
    29941 attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg
    30001 gaattgtcta gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt
    30061 accaatatct atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct
    30121 aaatggaacc ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag
    30181 tcagaactac aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa
    30241 gaagtgttgg aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa
    30301 ataatggaaa tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct
    30361 gctatttctg tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat
    30421 tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt
    30481 tataatgatt caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa
    30541 acgtctaagg tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta
    30601 attccttcat taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg
    30661 atggatgctg gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa
    30721 gcagttcctt tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg
    30781 aatgccagct gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct
    30841 gtgaaccgat taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac
    30901 aggcggagga acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac
    30961 tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat
    31021 cgtccctgat acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat
    31081 ttccatgcat aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa
    31141 aatcctcagt gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc
    31201 ggtagtgtac gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt
    31261 accaggtgtt accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa
    31321 agatgcggct tccttttttc ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca
    31381 agaagtagca gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa
    31441 gaaaacagtc ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc
    31501 agttttttta agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag
    31561 atattatgaa gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc
    31621 ttatatcaaa aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg
    31681 tgcaacttcg atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc
    31741 atatgttacg aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc
    31801 aaaaatgatt gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat
    31861 catgggtgat gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc
    31921 tgatgcttca aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg
    31981 gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac
    32041 aatttgactc cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac
    32101 caagagccaa aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa
    32161 aaaacggttc aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt
    32221 gaagagatca agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg
    32281 cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact
    32341 gtattgggta taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa
    32401 gcagttgcag tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa
    32461 ttgtcattag gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc
    32521 cttaaaaaag cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa
    32581 ttcgatttgg atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac
    32641 tacccagtga ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat
    32701 ctccctatct tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac
    32761 aatcagttat catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca
    32821 agcaataaaa atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc
    32881 aataatcttg cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa
    32941 ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg
    33001 gaagaatcgg gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac
    33061 gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac
    33121 ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca
    33181 actccgtcaa gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc
    33241 taaaaaccag taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat
    33301 tttaaaacgg acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat
    33361 tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg
    33421 tcgtggctga atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag
    33481 aagggattaa ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag
    33541 cagtacgaca agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc
    33601 cagcaaacat gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa
    33661 aggaaatcac cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac
    33721 ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg
    33781 gaatcaaaga tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta
    33841 caggacctaa aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg
    33901 tcaacgaaat ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa
    33961 aagattttgg tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc
    34021 atgataagca attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag
    34081 atatctctat tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg
    34141 gggatgcata cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc
    34201 agtcagaacc agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg
    34261 aacagatttt caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag
    34321 gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag
    34381 aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg
    34441 tatttacaaa tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat
    34501 tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag
    34561 aaacaacaaa cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg
    34621 aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt
    34681 ttgattaatt catcctggga ggaataagat atggaattct caattgataa taatatcaat
    34741 gacggcgcag taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga
    34801 atgattgaag aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca
    34861 ttaaaaaatt caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta
    34921 ggtgctggtt ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta
    34981 cgcgaagctt tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc
    35041 ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt
    35101 ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa
    35161 ggtatcgctc gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta
    35221 ttagaagttg tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt
    35281 ttacgacaag gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt
    35341 gactttgctg acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt
    35401 gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg
    35461 cttgaaacat ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat
    35521 atgacattgt tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat
    35581 gtaaatatca ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg
    35641 gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga
    35701 caagcacaaa tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca
    35761 acttcttcag aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt
    35821 cgtcctagag ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc
    35881 aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt
    35941 cgtaaaaaat aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca
    36001 agaaattcag gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc
    36061 tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa
    36121 ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc
    36181 tgaaataaag tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa
    36241 tgaaattgat tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg
    36301 gttagagaaa gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca
    36361 tggaatttca ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat
    36421 caaggttgtt gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa
    36481 agtgtttggt acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc
    36541 accatgtagt gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg
    36601 tgcaacattt atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa
    36661 tgccgctttt aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt
    36721 acgaatacca agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata
    36781 caacacagaa tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa
    36841 cggcgacttc acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa
    36901 ttgaactaca tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct
    36961 ctacacaacc taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt
    37021 ctgaagctat ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc
    37081 atttagtaga agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg
    37141 cactagatgg agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga
    37201 tggagattga cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag
    37261 aggaggaact acgatttata ctattgcact attacttcat ttacttcaac gggcagccta
    37321 tttctatagc attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca
    37381 atcagggcta ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg
    37441 tctgcctttg aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa
    37501 cttagccgta caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg
    37561 attgaagcaa caaaatcttt attctcagaa tagataggag gggaaaattt tggatgcaaa
    37621 tgtataccag cactttcggg cagatgaacg tacatttatc gattcggtag gcgattggat
    37681 tgaacaggtt caaggacaat atgcacccta tctgaccgaa tttcttgacc caagacaatc
    37741 ctatatttta gaaacactga ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg
    37801 atatgaacag gcagaaagaa aaagatgttt gattttcccc gattattacg aacctgtaga
    37861 ggaagatttt gaaacagcat tgtatgaaat ccattatcct agcaaatttg ccagcctaag
    37921 tcatgggaaa atccttggaa ctttagtggg aacggggatc aaacgctctt actttggcga
    37981 tatcatctcg gacggtgatc gctggcaagt atttattgca aatgaggtgg atcattttgt
    38041 tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac
    38101 agaaatgatt atgccgaaag atagctggac acaagaaaga ggaacagtta gttccatgcg
    38161 actagacagt gtgatctctg agatttataa tatttccaga cagcggtcca agcagctgat
    38221 cgaagctggt aaagtaaaag tgaactggac agaaaataca agacctgatt ttgtacttga
    38281 actattagat attatttcag tcagaggtta tggcagagtc cagatccaag aacttgaggg
    38341 gaagacgaaa aaagaaaagt acagggtact gttcggtgta ttacgtaaat aaaataaaaa
    38401 ggtggtttac tcatggcttt aactccatta gatatccaaa ataagacatt cccgacaaaa
    38461 atgcggggat ataatcaaga cgaagtagat gatttcttag atttagttgt tcgtgactat
    38521 gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa
    38581 cttgagtact tcaatgaatt aaaagatgct ttgaaccaat caatcatcgt agctcaagat
    38641 acggctgata aagtaaaaac aagtgcaagc aaggaatccg aagtgatcgt tacttctgca
    38701 caaaacaaag cagatgagtt agttgcgaac gctgaaaaac gtgcacatca attaacaact
    38761 gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa cagaaaaagc aagacagctt
    38821 gcgacagaaa ctgaagatct taagaaaaaa acacgagtat tccatcaacg tatcagcttg
    38881 atgcttgagt ctcaattaga acaagtgaaa agtccggaat gggatgaaat tttacaacca
    38941 ttctccagct acgtgactga cagccatgaa gtgatcaaag aagttttagc aaaacagctt
    39001 gacaatgaaa atgatactga tgtaaactca gacacaggag tcatagaagc acctatgacg
    39061 gcatttgata cacaggctat tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag
    39121 aacagaaaag cactttgtta ttcttaaagc gagccgatga gagtggaagt tcggtaagac
    39181 actgcatggt gaaagatcct ctacttgaga cctgcttttc cgaaattaga gtaagaagca
    39241 gcggctgacc tcgttattgg tcacagagag tttgcactag atagatgcaa ctaaatttgg
    39301 gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca
    39361 aataaaggag tgtatggttg aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa
    39421 ttccctatgc gtggtaattt gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa
    39481 aatcgaatct accaaaaaag acaagagttg aacgaaggaa aacctagctt tgttcttcat
    39541 gatggacctc catatgccaa tggtaatatc catttaggac attcattgaa taaaatcagt
    39601 aaagacatta tcattcgttc aaaatcaatg tctggctatc gtgcaccata tgttccagga
    39661 tgggatacac atggattgcc gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa
    39721 gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat
    39781 aagcaacgag cagatttcaa acgcctagga gttgcaggag attgggaaca tccttatgtt
    39841 acattagatc cttcttatga agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa
    39901 ggctatattt acaaaggatt gaaaccaatt tactggtctc cgtcaagtga gtcttcttta
    39961 gcggaagctg aaattgaata taaagatgta aaatctccat ctatttttgt agctttcaat
    40021 gtcaaagacg gcaaagatat tcttgatgaa gatacagctt ttgtcatttg gacgacaaca
    40081 ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc caaaatttac gtatgtattg
    40141 gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt tgctagaaac agtccaatca
    40201 gaaatcggtt gggaaaatgt aacagttcta aaagaatttg ctggacaaga aatggaatat
    40261 atgactgcaa gtcatccatt ctatgaacgt gaatcacttg tgattttagg cgatcatgtt
    40321 actttagacg ctggtactgg attagtccat actgctccag gacatgggga agatgactat
    40381 atcgctggta gcaaatataa actatctgta gtgtcacctg tagactcaaa aggattgttt
    40441 acagaagaag cacctggatt tgaaggtatc ttttatgaca aagcaaatcc tatgatcact
    40501 gatctattaa aagaaaaagg agctttgctg aagctagatt tctttactca tagttatcct
    40561 cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct
    40621 atcgataaat tccgtcagaa tattttggat gaagtagaaa aagtagagtg ggttatccca
    40681 tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg gcgattgggt aatttcccgt
    40741 cagcgtgcat ggggagtacc acttccgatt ttctatgctg aaaatggaga agcaatcatc
    40801 acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg aacatggatc aattatctgg
    40861 tttgaaaaag aagcgaaaga acttcttcct gaaggattta cccacccagg ttctcctaat
    40921 ggcgaattta caaaagaaaa agatatcatg gatgtctggt ttgattctgg ttcttctcat
    40981 gaagctgttt tacgtcagcg accagaatta tcattcccag cagatatgta tttagaagga
    41041 tcagaccaat atcgtggctg gttcaactca agtatcacga ctagtgtggc tatcaatggt
    41101 gtagcaccat acaaatcagt tatttctcaa ggatttgctt tagatggtga aggccgtaag
    41161 atgagtaaat ctttaggaaa catcattgca ccagaaaaag tgatcaaaca atttggtgca
    41221 gatatccttc gcttatgggt atctagtgta gattatgaag cagatgttcg tgtttctatg
    41281 gatatcttaa gtcaagtggc agaagtatat cgtaaaatca gaaatacgat gcgtttctta
    41341 ttagcgaata cagaagattt cgatcctaaa aaagatactg tttcgtacaa tgatttacgt
    41401 tctgtagaca aatatatgac cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa
    41461 gggtacgaca aatataactt catgcatatt taccgtacag ttatgaactt cttgacggtt
    41521 gatctatcgt cattctacct ggattttgct aaagatgtag tttatattga agcagaagat
    41581 gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa
    41641 ttattgactc ctatcattcc gcatacagca gaagaaatct ggtcattcct gaaagaagaa
    41701 gaggagtacg ttcagttaag cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa
    41761 ttgatggata cctggactgc tttcatggac ttccgtgata atgtcttaaa agcattggaa
    41821 gaagcaagaa acagcaaatt gattggtaaa tctttggaag caaaactaac tgtttatcca
    41881 aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag atattgcaca attattgatc
    41941 gtttcaccag actactttga agttatggca gcagatgaac aagtgccaga taacgctatg
    42001 gtatttgatg atgttgctat tacagtggaa aaagcagacg gggaaacttg cgatcgctgt
    42061 cgccaagttc gtaaagatgt aggagtagat gaaaaacttc ctcatctatg cggtcgttgt
    42121 gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg aaggattcga agaaaagtaa
    42181 aagggaaaag ggggtgtgac atgttctttg tcacaccccc ttttgttatc aatcgtttaa
    42241 ttttccacgt tcacgatacc agttatctgg ttgccagatc cattcgaatc cgtctttttc
    42301 aagtagttcg aaagctgctt taggtcccat tgatccagct ttgtagttgg gaaaatccgg
    42361 ttgtgtttgg tcccaagctt ttcgaatgac atccacgata tgccaagatt gagcgacttc
    42421 ttcccaatga gagaagttcg ttccatcacc attcaacgca tctaatagta atttttcgta
    42481 agcttctggg ctattttcaa ccatttctgc actatttcta aattccaatt tgattggttc
    42541 cgtttcaaat ccttgcccta cttctttccc attcaaggat aaagaaaatc cttcagttgg
    42601 ctgaatatag atcgttagaa cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac
    42661 agatgttttg aatacattaa caggaacttg tttgaatacg atgttgatcc gagtcccttt
    42721 ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca cctgaccaac ggaagttatc
    42781 gatagtgaat ttgcctgcga cgaatgtttc agtctctgat tgagcatcca ctttgtcttc
    42841 ttcgcgatag cccttgaatt gtacaccatc caattcacct tgagcatatt gcccgcgaac
    42901 gaagttttct aacacttctt cttcgttata aatgcggata gctttcaatg ctttgatctt
    42961 ttctgtacgg atttcttttt cggaaaatgc tactggcggt tccattgcaa gtaatgctac
    43021 aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc
    43081 cccgcgatct tctacaccga ggttttcgcc aaaagtaatt tggatattgt cgatatagcg
    43141 attgttccat tgagcctcaa aaatattatt ggcaaaacgg atagctgaga tgttttggat
    43201 catttctttt cctaaataat gatcaatcct gaaaatatct tgttctggga atacttctct
    43261 gatttgctgg ttcaattcaa aagcagactg gtaatcagaa ccgaacggtt tttctatgat
    43321 caaacgattg aagccatttt ttgtcataat attttgtgat ttcaaatgag aaacgatcgt
    43381 tccaaaaaat tgaggtgcca tagccaaata atagacacgg ttaccgttta attgatagtg
    43441 tccatctaat cggtcagaca attcttttaa tgtctgataa tgcgttgaat catttacatc
    43501 atgggattga tagtaaaaat ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa
    43561 tgcacggatt gtttctttga caacttcacg gtagtgctca tcgctccagg gacggcgagc
    43621 ggttccgatt acagcaaaat gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga
    43681 cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt
    43741 tttttcatta gacatgtgga tacactcctt tatattttat cgacaaaaat cgtgctggcc
    43801 gccttaaagc tgacggggat tttctttcta tcattttgga tagtgatcgg tccttcataa
    43861 gctgcgatgt caacgatctc atattcttcg ttgatattca ggtcaatagt gactaagtaa
    43921 tccaacaatt ctttttcatc aagcactcgt gcgatacgca cttttgtacc gacaggatag
    43981 gactccaatg tttgtcggcg ttcttcatgg acaacttcct cttttttagg aatcgatcct
    44041 ccatgaggac aatgttcagg atgattcaaa tagttttcta aatgatctgc aagatgttcc
    44101 gaagtcacat gttccaatac ttcagcatca tcatggactt cattccagct atagtctaaa
    44161 tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa tcagactgct agctttttgc
    44221 agtccgcttg cagtcaattg tacaccttga taaggggaat gttctacaag ctcttctttc
    44281 acaagtttag aaatcatttc actgacagat gcagctgaga catctagact agatacgatt
    44341 tgtttattgt tgactttcgt tgtatctcca cctagttcta aaatgatttt caaataatct
    44401 tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg
    44461 taacaaaaaa aacgttaatt gactattttt ctaaataaaa aacaaaggat ttttagacgt
    44521 ttttacaaca aaaaacgaaa gatgatagag aagactcatt ctttcgttta taaaggatat
    44581 atatatacat tgggagggat ctatttttca gataacaact tatatgaagt gtctgcagat
    44641 tcgtcataaa gagtttgttc gggataagaa aagataagat aaaaaccata ctgatcacgt
    44701 acgactaaac gatattcatc tatttctacc acagatgtgc caagagactg accgttgatc
    44761 aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt gctggctatc actaaagaac
    44821 catgtacctt gatactttga gagtccttct gacttttccg cttgtttttt ctttttcaag
    44881 taaataaaac ttgagagaac ggtaatgagt gaaccaagaa aatagatcat tttttttatc
    44941 accaaatcac tcctttatga gaaaataatc gcatagacat attctttctt ctttatttca
    45001 ttgtaccgaa taaaagctaa aatttctcta agactttcga ctggtttttt tactgttttt
    45061 ctacaaatta tgataggata catagtagga agaaaggtga cataatttga agtatcagca
    45121 agtggaaata gcttttttta ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa
    45181 agacggagaa ataattattg cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat
    45241 accaggcgct gaagtggcta ttgtatttgc tcctggacct aaacggaaaa ccgcctatga
    45301 tctgattgct gtaaaaaaga agggtgactg gtttaatatc gatagtcaat tgcctaatcg
    45361 gctggcaatt gatggtattt tagacggcac gatcctttta ccaggattaa ctggtccatt
    45421 tacggtatac aagcgtgaag taacttatct tcactccaaa tttgatattt atttagagac
    45481 ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg acacttgaaa ataaagcgat
    45541 tggtgctttt cctgatgcgc ccacactcag aggattgaaa catataggag aattaacgta
    45601 tgccgcacaa gatggttatg ccgcttatgt cttgtttatt gctcagttcg aacatcttca
    45661 tctggcaacg atccatgaag aaatgcaacc agcactagct gatatggttc gacatgcgca
    45721 gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta tcggcagatg aggtaactgt
    45781 caagcaggcc attccatttg atgtagatat cccttttgaa gatcctatac aatagaaaga
    45841 aggtaaaata atgatcaact taggtattat cggaacaaat tggattactc atcaatttgt
    45901 ccaagcagct ttaagcacaa aaaaatatga attgacagcg gtatattcta gacatttgga
    45961 aaaagcacag gaatttggtt ctcattatga aggggatatc gaattcgcaa cagatttgaa
    46021 gacttttttc aatattactc atatggacac catttatatt gcttctccga atagtcttca
    46081 ctttagtcaa gctaagcaag caattttggc aggaaaaaat gtgatagttg aaaagccagc
    46141 tttttcgacg ccaaaagaaa tggatgaaat catccatttg gcaaatgaac acaatgtgta
    46201 tttttttgaa gcagcaagaa atatccatga atcaggtttt aaaaagattg ccgattactt
    46261 accattaaaa gacgagataa tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta
    46321 tgaccaagta ttagatggag aagaaccgaa tatcttctcg actcatttct caggtggtgc
    46381 attgatggat ttaggtgttt accttgtata tgcagctgtt gcttggtttg gaatgccaaa
    46441 agaagtccat tatttcccaa gaaagatttc tactggtgta gatggtcttg gatgggggat
    46501 cttacgttat gagaattttg atgtatcgat ccagccaggt aaaatcggtg attcttattt
    46561 accgtcagag atttattttg actcaggaac gctagtgatg aatggagtca atgcaattga
    46621 acaagcacaa tttcatgatc gcgcgcataa agaaatcatt gatttggcgg taacagctgc
    46681 agaaaatcca atggcagatg aagcagagga ttttgccaat gtcatggctc atccgaaaga
    46741 tccagcttgg ggattgttgt atgaagagtg gatcgagcta gctagaaatg tgaaccaagt
    46801 gatttatgat ttaagaaaaa atggcggcat caaattcgat gcagataatg aggaagattt
    46861 ttaatgaacg aatttgtaca agtatttccc gaaaattggc agaaaaaata tgaggataaa
    46921 gggtttatct caccttctct tattcaacga gcagtttttc agccactaag taataaacaa
    46981 agtatagtag cggtttctcc tacaggttca ggtaaaacac tggcttatct ttggcctcta
    47041 ctactaaacg tggaaccagg tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa
    47101 ctagctattc aagtagcaga tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc
    47161 caatctttgg tgggcggagc caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca
    47221 gaaatcttga ttggtacacc aggaagaata ttggaattga tgaaagcaaa aaaaataaaa
    47281 gcacatcaag tgaaaacaat ggtatttgat gaagcagacc agctatttga cgctgggaac
    47341 agccaaatca ttgatcaaat tcttcatcaa gctccaacag agtatcagct agcttttttt
    47401 tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag
    47461 acaattgacg tgacagcaga ggacgattct cgaaaaggac tgagacatta ctttttacgt
    47521 gtaccgatac gaaagaaaga tgactacttg agacgtctca cgcatataga agattttcat
    47581 ggtctgattt tctttaatca actgactgaa ttaggcgtaa tggaagaaaa attgctgtat
    47641 agaggtgtac cggtaggaag tctggcctct gatcagaaca agctactccg aaaagcagcg
    47701 ctagaacaat tcaaaaagaa aatggtcaga gcacttttga ccacagatgt ggcggctaga
    47761 ggtttagata ttacaggtct tccttatgta gtgaatgcag aagttccgtt aagtgaagaa
    47821 gcttatttgc atcgttcggg aagaactggt cgaatgggta atgaaggaat agtaattaca
    47881 ctcgtacaag ataataatct aagagattta aaacggcttt tacgtccatc aaatataaca
    47941 cttgaagaga tttttttata cggaggtaag ctgcaaactg agcagccaac caaagaagat
    48001 gcagctcctg cagtaaataa aaagtatgcg aaaacttttt ctggaaagga gcagaaccca
    48061 gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat
    48121 aaaggagcaa ggcgcaaata agcgcctttt tatgctatga ttatgaaaac gggaacaaaa
    48181 ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga agagatcttg cagctattga
    48241 cagaaaacca acaaggattg acagcaagtg atgtagctga gcgcttaact atcgatcgaa
    48301 gcaatgcaag ccgatattta agtgaactat acaaggccca tcatattgta aaaaccgctg
    48361 gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg
    48421 actcttctac gcaagtgact tttgaaacac tcgttggaga aaatgattct ttaaaagtca
    48481 gcatacagca ggcaaaagct gcgattcttt atccaccgcg ggggttgcat acgattattt
    48541 ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg
    48601 attcagagat gctttctgca gatgcgccat ttgtttcttt caattgtgcg gattatgatc
    48661 aaaatccgca actgctcttt ggacacatct ttgggatcaa aaaaggggct tacacaggag
    48721 cagctcaaga tagtcccggt ctaatagcca aagcagacgg cggtatattg tttttagatg
    48781 agatccaccg tttaccgccg gaagggcagg aaatgctgtt tactttcatt gataaaggta
    48841 tttaccgtcc gcttggagaa agtgcacaag tacatgaagc atctgttcag atcattgggg
    48901 cgacgactga atcttcagaa agtttcttat ctacgttcaa tcgacggatt cctatggcaa
    48961 tcaccttgcc gaatcttgct tcacgttcac tggatgagcg ttatgaaatc atctctcttt
    49021 ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc
    49081 ttgccttcat gctttatgat gcggaaggaa atatcggtca agtgaaacga gacttgaaac
    49141 tagtctgtgc aaaatctttt ttacattacc gtactcatca tgagaaaaag ctaatcattc
    49201 gaaaagaaga actttccctt caagtacaaa aaggattact gaaaatcaag gaaataccgg
    49261 aacgtctcga ccgattcatg gacagcaaaa gtcagtattt gacatttgaa ccaggatttg
    49321 ctgatgttgt ctggtcacaa gatccagaac gaaacatgca ggtctataac gatattgagg
    49381 aaaaagtcac ttctctttcc gaaaccggtg tggagaacat tgatctagag accctcattt
    49441 caaaagatgt ggatgcttat ttccagacat atgtcaaaga attgactaaa agttcgattc
    49501 ccaaagatct tttgccagat gaaatctggc ggttaaccaa tcaattgtat gatgtggctg
    49561 aaaaacggtt agaccgtatt tataatgaaa aagctcgttt tgcttttgct ttgcatctgc
    49621 aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt ccacccagat ttgaataacg
    49681 tacggaaaaa ttttaagagg gaattccagg tagcaatcga tctttcaacg atcattgaag
    49741 aagaagaaca agttgagatt ccttttgatg aaatcggctt tattagtatg tttctttcta
    49801 tcaatctagg agaaagtgag cctgttaaag aaaacaaagt agatgttctc atattgatgc
    49861 acggcagaca gactgcttca agcatgttgg agatggcaca agaactatta ggaacaaaag
    49921 tagggaaagc ttttaatatg ccactggtta tggaagttca aacaatgtat gaagaagtgt
    49981 tacaatatgt caaaaactat caagagcatt tgacgaatgg cctactcttg ttaacagata
    50041 tgggatcatt aaacaccttc agtgatttga tttatgaaga gactggcatt cggacgaaag
    50101 ctatcacgat gacaagtacc atgattgtga tagaagcgat acggatggca agtgttggaa
    50161 gaagcttaga agatatttat caaaatatcc agctttcctt tgaatcgatc gtacgtgatc
    50221 agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa agccgtaatc gtgacatgct
    50281 ttactggtga aggagtagct gccaaattat atcagcgtat tatgccagtg attaatcaaa
    50341 ataaggtaga actgatccag atgcagtttt tagaacgtga atcattcaaa aaacacatcg
    50401 atgccttatt ggaagaatat gagatcaaag caattgcggg aacagttgat gttgattatc
    50461 aaaatatccc atttttttca gcctatgacg tttttgatga tgaaaaacta aatattttaa
    50521 agcgtatagc tgatgatgag atcccgacag ctaaaattgt cgattcactt gctggatcat
    50581 taagcgatgt cttttctatt gatgaactga tccgttcatt gcaaaagaca gttcagcaga
    50641 tccagaccga tttgtcgatt atcgtggaac ctggagtaga tactggaatc gttatccatt
    50701 tagccttcat gttagacgga ttgatcaaac aagaaagcca tcggcaattt gagaaactgt
    50761 ctattttctc aaaaatttat cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac
    50821 tagaacgaaa atatcaggta aaaattccag aagatgatat cgcatttctt acacaaatgt
    50881 ttcttgaaaa taaaataaaa aaatgaacac actaatcata ctgtttacac actaaacatc
    50941 tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag ctcttttttt
    51001 tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg
    51061 gcaaaaaaaa caatcatgtt agtttgttca gcagggatga gtacgagttt attggtcaca
    51121 aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg atatttttgc ggtctctgcc
    51181 tcagatgcag ataataattt agaatctaag aatgtcgatg tattattatt aggtcctcaa
    51241 gtacgtttca tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat tccattagat
    51301 gtcatcaata tgcaagacta tggcatgatg aacggagaaa aagttcttgc acaagcagaa
    51361 aatctaatga aataaatcaa gtatgcagtt gggggtataa caacgtggaa gatcaacaaa
    51421 atctagaagc catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg
    51481 aagcgattgc ggctgctaaa atcggtgatt ttgagttagc ggataggaaa atcgctgatg
    51541 cagaagaatc acttatacaa gcgcatcatg cacaaacaga catgttgaca caagaagcac
    51601 aaggaaatca tatgcaagtg acattattga ctgttcacag tcaagatcac ttaatgacat
    51661 ctattgcgtt tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg
    51721 aataaataca acttcttttt tgaacggaag aagaaaaatt tttgaacgga ttttttattt
    51781 tgttataagg agaactctct ttatataaaa aacttatagg aggaagtaaa aaatgaacgg
    51841 actatcaagt tggatggaga aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa
    51901 acatttagtt gctttgcgtg atgcttttat cggcatactg cccgcaacaa tggcgggatc
    51961 agtcgctgta atgatcaatg caattattcg tgatttaccg caacagttta acagtaccta
    52021 tgctgacagt ttagcagcag acaaaggaat ctttgccgta ttgaatgtca tcatcggaat
    52081 caatggtttt gtctggaacg gtacactagc aatcgccgga ttgatctttg ccttttcttg
    52141 ggggtataat cttgcccgag cttatggagt aaatgatcta gctgggggga tcgttggttt
    52201 agctaccttg attcaaggta tttcctttgg tccagcatta tcagcagcat tagatgtgaa
    52261 tgttccttca aatgtcgcag atgcaatcaa tagttctgag attggtgcaa cgattgcaga
    52321 tggcacactg tcagttggat tatggggttg gctaaaacta gatcatttga atggaaacgc
    52381 ttattttaca gttatgatca tgggagctat ttcagtcatt attttttgta aattgatgct
    52441 tgccaacatt acgatcaaaa tgcctgattc agtaccacca gcagtatcta aagcatttgc
    52501 agccatttta ccagcgacga tcgctttata cgtcattgct attatcaatt atatcgtagg
    52561 tcgtgtagct gatgggcaac tgatcattga tttggtgcaa aaatatattg cagaaccatt
    52621 cctaggatta tctcaagggt tgggagcagt attgatcgtc acagtatttg ttcaagtatt
    52681 ctggttcttt ggtattcatg gatcaaatgt attggcacca gtgctagagg gaatctgggg
    52741 acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt tataacggaa aaactggtac
    52801 accagccgtg ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt
    52861 cgatgctttt gcatggtttg gcggatcagg cggaacgatc gtcttactga tagctatctt
    52921 gattttctct aaacgtgcag attatctaac agtagctaaa ttatccattg ggcctggtat
    52981 cttcaatatc aacgaaccag tgatgtttgg tctgccaatc gtattgaatg caatcatgtt
    53041 tatcccattc ttgattgctc cagtagtagc aacagcaatt ggatggacag ctacttatct
    53101 tggcctagtc gctccggtat cgcagcaagt cacatgggta gtaccgccgt tcttattgtc
    53161 attcttagca acaggagcgg attggcgggc accgatcgta acattagtat gtatggtcgt
    53221 gactttcttg atttgggcac catttgtcat ctctgcaaac aaaatggacc ctgccgcagg
    53281 cgaacaacaa taagatgaac gtgaggagcg ggacgcttta agtttcgttc ctcataatga
    53341 attttcgagg aggaactgaa atgattacaa ttgccagtgc aatgcaacaa gatgaaatca
    53401 aagaattgtt agaaggatac aatgaagaag gaatgacttt tacatttaaa gaaaaacaag
    53461 gaatcaagct gttctttgaa acaagtgcag aagatccgga agttgcggct aaaaaagcaa
    53521 aggaactaat caaagcacag ccttggggaa cagtccttta ttttcaatca acagctgttt
    53581 agaaaatggt ttaaaaagat aggatggttg agatgataaa aaacggttta tttttatgta
    53641 gtattggttc tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt
    53701 atagtatggt cacaggtctt tttctaacag gattaggaat ctatttcttc atgaagggga
    53761 aaaaaagaga agaagcaaag gagaaaaata actaatggaa atcacgaaaa aattaaatat
    53821 tccagcatcg tttttttacg ataaagttat ggactctgtc cttttcgatg tacgtaaagc
    53881 gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg gagtacgtca aacaattctc
    53941 gcaaaataac cgggctcgca taaaaatcga agaagtcatt gaaaaccagt cttataagtt
    54001 tcgtacatcg acaacgaaga atgaattcgt cgttcattat caaatcaaac cattggacga
    54061 ttcgacctgt gaggttcatt acacagaaaa aatggagtct tatggcatgc ttcaaaagtt
    54121 gaatgatatg cttttaggaa caatcttgat gtattttaaa cggagacgtt tcaaaaaaat
    54181 gctagatatg atcgaagcaa cgtattaaat caatgaaaca tggtactatt tagttattga
    54241 aaaatgaagt aagctagaag gaatttttca taggaagata gatctttcct gcgaatcctc
    54301 actagctttt ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga gctaggtgta
    54361 tcttcgattg aggaaatgtt tgatttagcc acttatgcat ttaatggggc agatacacct
    54421 gaaaggcgag aacgttttcg tacacttgcg gagaattctt ggaattacgg attttttgat
    54481 gaggaggatc ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa tttttacgga
    54541 caagagtacc taatggccgg cattggctat gttgcttctt atccagaagc aaggggacaa
    54601 ggcgggatca atcagatcat ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc
    54661 ttatcttatc tggcgccatt ctcctatccc ttctatcggc gctatggcta tgaacaatca
    54721 tttgataaaa tcagttatca actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct
    54781 ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg caatcagaag aatttatgaa
    54841 cagatgccgg aaaaccaacg agctggtctg aagcgcgaag actggtggta tacgtataaa
    54901 ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg atgataatga ggaagcaacg
    54961 ggatatatcg tttatcagct tgcaacgtct tcctttatta ttgcggagtg gggatttttg
    55021 gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa
    55081 acatttgaat attcatgcgg ctacggcggt aatgatcaaa attatctatt agaaaatcca
    55141 tttgcttctg taatgatcac accttatatg atggcgagga tcgtagatat ttctttgttt
    55201 ttggaaaatt atccatttaa aaagagacaa gctgcatttg ccctacaaat agaagaagat
    55261 cattatgcga aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat
    55321 ctctcaaaag tagaacaaac aaaactccca atcgttcgag gaagtatcca acatatcacg
    55381 cagctattat tagggtatcg taaagtggaa gagttggtat tccaagaaag aatacaggtg
    55441 acaaaagaat atgaagaaac gttaagctca atcttgccac agcaacagcc aattcttaat
    55501 gactattttt gataaaaaac gggtgatgac aatatttttg tcaccaccct tttgtttttc
    55561 atttggcatt tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat ttgaattttt
    55621 tgttttcttt tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct
    55681 tttcccgatt ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat
    55741 cattcattgg aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta
    55801 aggactagtg gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc
    55861 aagtaaaaaa caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga
    55921 tagagcaatc gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtataaa
    55981 taacccctgt atcctttgcg gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca
    56041 aactagttat catttttata taaactatcg aacacagtat tgattttttc tttatcctga
    56101 tcttcaagct ctcgaacaat atgaagataa gtagacattg tagtttctaa actgctatgt
    56161 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgt
    56221 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
    56281 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
    56341 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
    56401 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
    56461 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
    56521 tcttcgaaat cgatatcttc ccatgttaat cctagaagtc cagcaaatcg cattccagtg
    56581 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
    56641 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
    56701 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
    56761 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
    56821 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
    56881 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
    56941 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
    57001 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
    57061 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
    57121 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
    57181 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
    57241 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
    57301 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
    57361 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
    57421 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
    57481 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
    57541 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
    57601 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
    57661 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
    57721 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
    57781 tcgaagtaaa caattcttat taagattttt atacatatct attttatttt gtgcctttga
    57841 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
    57901 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
    57961 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
    58021 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
    58081 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
    58141 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
    58201 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
    58261 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
    58321 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
    58381 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
    58441 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
    58501 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
    58561 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
    58621 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
    58681 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
    58741 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
    58801 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
    58861 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
    58921 tcttaaaatc gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt
    58981 taatttcatc tgcacttgaa atttgtgttt ctttggaatt attatctaat atagattgtt
    59041 gcttctctat atattttttt aaattttcca tttcattcca atattttttt gtaaacataa
    59101 cggtgttttc atctttcgtg gccgctaata tacctccgcg actttcgttt ccgcctagca
    59161 aggtaaattg tatataacca ttactcatac ctggtttttt taattgtacg gctgatatat
    59221 ttttaaaggg gatagttttt tctcctttaa taccttgatt aactaaattt ataaatcctt
    59281 ttctaataat tgaaatagaa tcatcagtta ctttaatcaa ggtttttcct ggagatttta
    59341 ttaaaatctc tttttccatt ttttcttcct catttctgtg atatgatttt ttttgtagaa
    59401 gctcatatat gaggaaagag cccgtgttgc agcacgggct tttttaatgt tttgctgaaa
    59461 tcggtttttt taataattct tgatgtattc atacatattg ccttgcgtaa gaatattttt
    59521 tcttaaaata gcattggcag acagcataac aagtgtatct gagcttattc tgaatagaat
    59581 aatactccat aaatttttcg agattaaact gagattcatc agtcagttcg ttctcaatat
    59641 agatatttaa taaaattaga atagctattt tatctgcttc tgtttcaaat tttgagtgaa
    59701 aagttgtaga agtatcgtac aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa
    59761 gttcatgaga taaatgaaag gcttctgcag tttcaccgta tagattttca ttcaaaaaaa
    59821 tgattctggg ttttggatag tagaaaccag gctctttcat ctccatataa actactttca
    59881 aattgtattc gctcaacatt tctttcaatt tcaaatacat acaaaccatc actccaacta
    59941 ttcattttcc tctaaagctt tagcaattgc aatcgcttta cgcattgtct ccttagatat
    60001 ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat aaatccacat gtttaggggt
    60061 ttctctttct tctctaccta gaaggtagtc tacagagaca tcgaaatagt cagcaatccg
    60121 acttaattca tctgaattag gagtattatt tttccacttt gctagataac cgttagaata
    60181 tccaaaatta atttctaact gccgtataga tatctttttc ctcttcgcca agtcttttat
    60241 tatttcatag gtattcatcg ataaaacaac ctttctaaat gtttacaaaa aaagtttaga
    60301 aaaatacgca gaaataattt gactaattct gagtaatgag ctatactata tcttgtaaac
    60361 agatttaaca actaaaaaga caacaaaaaa cactattgat atgtaaatgc agaccgccaa
    60421 gaaagcttta aaatcaatgt ttttatgtct tatttaatta tgctcttagt atagagtatt
    60481 actcagatac tgtcaattga atttagaaaa aagttgttaa atttgtttac gaatataaaa
    60541 gaaaggaatg agaaatatga acacaccgca aatttttaat ttcgaacaaa acgaagttcg
    60601 gacaatttta gtaaatgatg aaccatattt tgtaggcaaa gacgttgcga gtgttttggg
    60661 ttattcaaac actaaagatg ctttgtcgcg gcatgtagat ttggaagata agatggggtc
    60721 gcgaatcacg acctcaggtc aatcaagaga gatgacaatc atcaacgaat ctggtttgta
    60781 cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag
    60841 tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa aagtagaaga
    60901 agctttgctt aatccagata caatcattca attagcaact caactaaaag aagaaagaac
    60961 tggaagatta atcgcagaac aaaagattgc agagtacgaa cctaaaatct cctatttaga
    61021 tagcatatta tcttctacag attcagtaac aattagtcag attgcagcag attatgggat
    61081 gtctccacaa cagatgaata aattacttca taaactaggt attcagaaaa aagtcggtaa
    61141 ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac acaaaatctc atacaactga
    61201 gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat accaaatgga cacagaaagg
    61261 gcgtctattt atctacgaat tactaaaaaa agaaggatat taccctcaaa tggatttaga
    61321 ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat
    61381 agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct
    61441 gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct
    61501 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta aagggaggaa
    61561 cagctaatgc aatatctaga agcaaaaatc ccaattccag aaggctatgt aattatctcc
    61621 caagtggatt atgaggagtt aaaaaaagct gatgatactg gtagatggat gacgttgcca
    61681 gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt tttaaagaac
    61741 ccaagatata gaaatattat cgatatcgag aaaaacaaaa atggatttgt ctattatcca
    61801 gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt tttagaaaca
    61861 aatttttcgg aaatcttaag gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt
    61921 cagcgcaccg ctaatcatct acgctttagg actttgggga agcag
//
LOCUS       DASJTM010000004        61238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJTM010000004 DASJTM010000000
VERSION     DASJTM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.004"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..290)
                     /locus_tag="U4B29_000222"
                     /pseudo
     CDS             complement(1..290)
                     /locus_tag="U4B29_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(544..2179)
                     /locus_tag="U4B29_000223"
                     /pseudo
     CDS             complement(544..2179)
                     /locus_tag="U4B29_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2502..2981)
                     /gene="rlmH"
                     /locus_tag="U4B29_000224"
     CDS             complement(2502..2981)
                     /gene="rlmH"
                     /locus_tag="U4B29_000224"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN2105198.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3409..4698
                     /locus_tag="U4B29_000225"
     CDS             3409..4698
                     /locus_tag="U4B29_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN2105199.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4737..5342)
                     /locus_tag="U4B29_000226"
     CDS             complement(4737..5342)
                     /locus_tag="U4B29_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN2105200.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5547..6011)
                     /locus_tag="U4B29_000227"
     CDS             complement(5547..6011)
                     /locus_tag="U4B29_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2105201.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6033..6353)
                     /locus_tag="U4B29_000228"
     CDS             complement(6033..6353)
                     /locus_tag="U4B29_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2105202.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6497..7576)
                     /gene="pepA"
                     /locus_tag="U4B29_000229"
     CDS             complement(6497..7576)
                     /gene="pepA"
                     /locus_tag="U4B29_000229"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN2105203.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7776..8126
                     /locus_tag="U4B29_000230"
     CDS             7776..8126
                     /locus_tag="U4B29_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2105204.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8349..9296
                     /locus_tag="U4B29_000231"
     CDS             8349..9296
                     /locus_tag="U4B29_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2105205.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9353..10771)
                     /gene="pepV"
                     /locus_tag="U4B29_000232"
     CDS             complement(9353..10771)
                     /gene="pepV"
                     /locus_tag="U4B29_000232"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN2105206.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10920..11765
                     /locus_tag="U4B29_000233"
     CDS             10920..11765
                     /locus_tag="U4B29_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN2105207.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11769..12209
                     /locus_tag="U4B29_000234"
     CDS             11769..12209
                     /locus_tag="U4B29_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN2105208.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12259..12453
                     /locus_tag="U4B29_000235"
     CDS             12259..12453
                     /locus_tag="U4B29_000235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105209.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12589..13275
                     /locus_tag="U4B29_000236"
     CDS             12589..13275
                     /locus_tag="U4B29_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2105210.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13683..14372)
                     /locus_tag="U4B29_000237"
     CDS             complement(13683..14372)
                     /locus_tag="U4B29_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105211.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14347..16476)
                     /locus_tag="U4B29_000238"
     CDS             complement(14347..16476)
                     /locus_tag="U4B29_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN2105212.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16524..17861)
                     /locus_tag="U4B29_000239"
     CDS             complement(16524..17861)
                     /locus_tag="U4B29_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN2105213.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18113..18472)
                     /gene="rplT"
                     /locus_tag="U4B29_000240"
     CDS             complement(18113..18472)
                     /gene="rplT"
                     /locus_tag="U4B29_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN2105214.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18525..18725)
                     /gene="rpmI"
                     /locus_tag="U4B29_000241"
     CDS             complement(18525..18725)
                     /gene="rpmI"
                     /locus_tag="U4B29_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN2105215.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18797..19321)
                     /gene="infC"
                     /locus_tag="U4B29_000242"
     CDS             complement(18797..19321)
                     /gene="infC"
                     /locus_tag="U4B29_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN2105216.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19365..19490)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19641..20273
                     /locus_tag="U4B29_000243"
     CDS             19641..20273
                     /locus_tag="U4B29_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2105217.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20275..21180)
                     /locus_tag="U4B29_000244"
     CDS             complement(20275..21180)
                     /locus_tag="U4B29_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2105218.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21265..21924)
                     /locus_tag="U4B29_000245"
     CDS             complement(21265..21924)
                     /locus_tag="U4B29_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2105219.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22240..23184
                     /gene="mvk"
                     /locus_tag="U4B29_000246"
     CDS             22240..23184
                     /gene="mvk"
                     /locus_tag="U4B29_000246"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN2105220.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23177..24154
                     /gene="mvaD"
                     /locus_tag="U4B29_000247"
     CDS             23177..24154
                     /gene="mvaD"
                     /locus_tag="U4B29_000247"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN2105221.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24167..25252
                     /locus_tag="U4B29_000248"
     CDS             24167..25252
                     /locus_tag="U4B29_000248"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN2105222.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25249..26313
                     /locus_tag="U4B29_000249"
     CDS             25249..26313
                     /locus_tag="U4B29_000249"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN2105223.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26562..27365)
                     /locus_tag="U4B29_000250"
     CDS             complement(26562..27365)
                     /locus_tag="U4B29_000250"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN2105224.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27385..28227)
                     /gene="kduI"
                     /locus_tag="U4B29_000251"
     CDS             complement(27385..28227)
                     /gene="kduI"
                     /locus_tag="U4B29_000251"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN2105225.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28243..29250)
                     /locus_tag="U4B29_000252"
     CDS             complement(28243..29250)
                     /locus_tag="U4B29_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN2105226.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29257..29901)
                     /locus_tag="U4B29_000253"
     CDS             complement(29257..29901)
                     /locus_tag="U4B29_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN2105227.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30001..30759)
                     /locus_tag="U4B29_000254"
     CDS             complement(30001..30759)
                     /locus_tag="U4B29_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN2105228.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30886..32037)
                     /gene="fucO"
                     /locus_tag="U4B29_000255"
     CDS             complement(30886..32037)
                     /gene="fucO"
                     /locus_tag="U4B29_000255"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN2105229.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32119..32430)
                     /gene="rhaM"
                     /locus_tag="U4B29_000256"
     CDS             complement(32119..32430)
                     /gene="rhaM"
                     /locus_tag="U4B29_000256"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN2105230.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32432..33277)
                     /gene="rhaD"
                     /locus_tag="U4B29_000257"
     CDS             complement(32432..33277)
                     /gene="rhaD"
                     /locus_tag="U4B29_000257"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN2105231.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33291..34547)
                     /gene="rhaA"
                     /locus_tag="U4B29_000258"
     CDS             complement(33291..34547)
                     /gene="rhaA"
                     /locus_tag="U4B29_000258"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN2105232.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34544..36004)
                     /gene="rhaB"
                     /locus_tag="U4B29_000259"
     CDS             complement(34544..36004)
                     /gene="rhaB"
                     /locus_tag="U4B29_000259"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN2105233.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36157..37026
                     /locus_tag="U4B29_000260"
     CDS             36157..37026
                     /locus_tag="U4B29_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN2105234.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37152..38051)
                     /locus_tag="U4B29_000261"
     CDS             complement(37152..38051)
                     /locus_tag="U4B29_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105235.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38216..39040)
                     /locus_tag="U4B29_000262"
     CDS             complement(38216..39040)
                     /locus_tag="U4B29_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2105236.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(39051..40346)
                     /locus_tag="U4B29_000263"
     CDS             complement(39051..40346)
                     /locus_tag="U4B29_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN2105237.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40362..40841)
                     /locus_tag="U4B29_000264"
     CDS             complement(40362..40841)
                     /locus_tag="U4B29_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN2105238.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40847..41875)
                     /locus_tag="U4B29_000265"
     CDS             complement(40847..41875)
                     /locus_tag="U4B29_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN2105239.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41892..42998)
                     /locus_tag="U4B29_000266"
     CDS             complement(41892..42998)
                     /locus_tag="U4B29_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2105240.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43227..45824)
                     /gene="adhE"
                     /locus_tag="U4B29_000267"
                     /gene_synonym="adhC"
     CDS             complement(43227..45824)
                     /gene="adhE"
                     /locus_tag="U4B29_000267"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN2105241.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(46129..46509)
                     /gene="yajC"
                     /locus_tag="U4B29_000268"
     CDS             complement(46129..46509)
                     /gene="yajC"
                     /locus_tag="U4B29_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN2105242.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46606..47751)
                     /gene="tgt"
                     /locus_tag="U4B29_000269"
     CDS             complement(46606..47751)
                     /gene="tgt"
                     /locus_tag="U4B29_000269"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN2105243.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47969..48667
                     /locus_tag="U4B29_000270"
     CDS             47969..48667
                     /locus_tag="U4B29_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2105244.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48835..51474)
                     /locus_tag="U4B29_000271"
     CDS             complement(48835..51474)
                     /locus_tag="U4B29_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2105245.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(52093..53124)
                     /gene="queA"
                     /locus_tag="U4B29_000272"
     CDS             complement(52093..53124)
                     /gene="queA"
                     /locus_tag="U4B29_000272"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN2105246.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53317..54491
                     /locus_tag="U4B29_000273"
                     /pseudo
     CDS             53317..54491
                     /locus_tag="U4B29_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            54495..55136
                     /locus_tag="U4B29_000274"
     CDS             54495..55136
                     /locus_tag="U4B29_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105247.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            55133..56050
                     /locus_tag="U4B29_000275"
                     /pseudo
     CDS             55133..56050
                     /locus_tag="U4B29_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            56047..56718
                     /locus_tag="U4B29_000276"
     CDS             56047..56718
                     /locus_tag="U4B29_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105248.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57259..57747)
                     /locus_tag="U4B29_000277"
     CDS             complement(57259..57747)
                     /locus_tag="U4B29_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN2105249.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57948..58214)
                     /locus_tag="U4B29_000278"
     CDS             complement(57948..58214)
                     /locus_tag="U4B29_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2105250.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58214..58477)
                     /locus_tag="U4B29_000279"
     CDS             complement(58214..58477)
                     /locus_tag="U4B29_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2105251.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58953..59138
                     /locus_tag="U4B29_000280"
     CDS             58953..59138
                     /locus_tag="U4B29_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2105252.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(59280..61103)
                     /locus_tag="U4B29_000281"
     CDS             complement(59280..61103)
                     /locus_tag="U4B29_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN2105253.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT        17753 a        13020 c        11185 g        19280 t
ORIGIN      
        1 atcagctcat catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat
       61 ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
      121 ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc atgtaatttt
      181 ttctcagaaa caaaccactc attggaaaag acatgaatca aatcatcact tacttgtgta
      241 aaatagcgac gtttaatgac aaagatatct tcattgctat ctagtgtacc ttcatttctt
      301 tgtgcttaaa ttcaaagata attttctcaa taaagatttt tgattccagt aagattcctt
      361 tactcctttt acaacatagc gttgtccttt gttttgaaac atcttcttcc cctctttgcc
      421 attaaaaata gcccaattct ctataaagaa gattaagctt cattattata atataaagtt
      481 taaatttggt ttattactta ctttttctta ttctctagta agctaatatt gaaaaaaatg
      541 taatcagatc attattttta tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa
      601 gaggtaacta cataaactaa ttaaaaaagc taacttcata gttggaggat aaatggaaga
      661 aacttcaatg attgtttctc cttgtgttcc ttcaatcgac actagtccta ttatcaatct
      721 ctcttttttg tccatttact tttgcgactg ttccccaata atcaaataca ggaagcgtta
      781 ttttttgtcc tttagattca tgtactgtat agactatttt cgaatccatc acttgataag
      841 gcatagtatg ccattccttg ccaattctta cttcatgatt ctgcaaatgt tctttgtatt
      901 gtaatgcttc cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa
      961 taagattttc ttgattcatt accaagtgat ctctctgagt gacaacttta tgttcatagt
     1021 ttgcttttgt tgcagcagat acagccaaca gtaataaaac aattgccgtt atattaacta
     1081 cttttctttg cttagctgat gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa
     1141 acagtacttg aaagcctaag attcgccaag gaaattgaat catttgaatg ggtgtatttt
     1201 gtaataaaga ccatggaaaa aatttggtgg ataagagcca agagactacc gcactaaggt
     1261 aaatcacttt ttctttgcga ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa
     1321 taataccaat gattcctacc gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac
     1381 tatccgagac atttaatgct tggtgttgta aggtcgtttt atctggctca ttaatcgatt
     1441 gaaccatgat ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga atcgtcattc
     1501 ctactgcctt tgacaactcc acaatcaacc ggatgctttc tttttttctt gtttcactta
     1561 ctacaaagcc gcctaagact agtaagccaa cggctattac actcataaaa gtggaaagaa
     1621 catgtgtata aatgagtaag ctcatactga ctgctaatgg aattgcacat ggctttttct
     1681 cataaatcag tgcgtaagca taatacataa ctatcggtat aaccgtaagt gcaatcgctt
     1741 cagcaatagc agaacgatga taaatatcaa tcgtacggta cccataaaat gtatataaac
     1801 agctaaatat aaaagccgca tcgattcttt ttaaaaattt tagtccataa tgaaacatca
     1861 gcatgatagt acaaacattc aatagccaaa catataagat ataactaacg attaaattat
     1921 tacttatcca ataaaaaatg actgccggaa aaaaggtcaa gtagggataa aaataattca
     1981 ctccgctccc ataatgatta aacgtcgtaa aattgattgg accagaaaaa attgaagata
     2041 accctttgac tctagcaaaa tggaaggcaa tatcccccga tgtagaaata gtatgaacat
     2101 ttctaaaata attgattgaa taaagtccag caataattgc taggagaatg gacaatatta
     2161 tttttttctt tacttgcatt acttaattat atactccttt catggattgc tttatctagt
     2221 ataacttaaa ttttaaataa atcgttattt atttttactc tcacttaaaa aagcactaaa
     2281 attataagag agggaggtaa tggtctgaac accaagaatt gttccaacat cgaattgctt
     2341 aactaatgag ttcaaagctt aattatgtgg ctcttatatt taccgagcgt ggattgcgat
     2401 ctctttagcg aaacaaacat ctttcataaa aaaaacagcc atatccgctt tcttggatat
     2461 gactatttac tactacttta ctatctttca ttcttcttgt ctcacttatg ataaggcgcc
     2521 cccgcattga tccggaaggc acggtagatt tgctcgacaa ggatcagacg catcaattga
     2581 tgtggatacg tcatttttcc aaaagagatc tgagcattgc ttcttttcat gacggcttcg
     2641 ctgagtccta aagagccgcc gataacaaaa acaaggtggc tttttccttg gatacctaat
     2701 tggtcgattt gtttagcaaa agattcactt gtaggatttt tcccttcaat tgctaaggca
     2761 tagacgtatt cttgttcttt gattttcgct aagatccgtt ccccttcttt ttcttttact
     2821 tgtctcattt gcgcttcgct taaattctcc ggggcttttt cgtcaggaac ttcgatcaat
     2881 tcgatttttg cataggcatt cagccgtttg acatactcat tgataccttg gatcaggtat
     2941 ttttctttta attttcctac tgagataatt ttgatattca tgttttctct cctttggtct
     3001 tcatgctcac tttgtttatt ataatcaatt ttttcgtcta tatgtttatt ttggctattt
     3061 ttgttaaaaa gatttcccca acagttatgc acaacatcca caggtttatc cacagaaaac
     3121 agtgttattt taccttattt catcacattc attaggcaaa catacgtttt ataagaattt
     3181 atccacttta tacacaggct gtggatatgt tttatccctt atttttccaa tcctatccgc
     3241 gtagttttac acagtatctg tggaaaaaga aaatcaaata ttttcatctg aaaacaatga
     3301 tttttcctaa agtttttcat aaaaagttca cagtctcaaa gtagactaaa gtcaacaaat
     3361 aagactatac taacagtaga taaatgaatg taattggagg aggaacacat ggatagaaaa
     3421 aatgtgacac caaagatgaa aaaaagtaaa aataatagcc tctggcgtaa gttaggtctg
     3481 gggcttgttg gagggatcgt tggaggacta ctgacagctg gcatttttta tgcagcgatg
     3541 ggcactggca acaatagttc tactgctaca agcggcaatc agaattctgc cggtgaaaca
     3601 gttgtggaaa atgtcaaagt caatgtagat tctgatatca ccaaagcggt agataaagtt
     3661 caagacgctg tcgtttctgt tatcaactta caaagtcaaa accaatcaag cggttttggc
     3721 ggactattcg ggcagcaaga agaaagtagc agctcagatg atagtaattt ggaagcttat
     3781 agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata ctgcttatgt agtcacgaat
     3841 aatcacgtag tagatggcca gcaaggtctt gaagtcttga tgaaagacgg tacgaaggta
     3901 aaagctgaat tagttggaac agatgcttat tcagatttag cggtgttgaa gatcaattca
     3961 gacaaagtcg aaacagttgc ctcttttggt gactcaagtg ctctgaaggt tggtgagcct
     4021 gcgattgcga ttggttcccc actaggatct gaatatgcaa attctgtcac ttcaggaatc
     4081 atttcttcct tgaaccgaca agtgacaagt acgaatgaat cgaacgaaac agtcaatatc
     4141 aatgcgatcc aaacagatgc agcaatcaac cctggaaatt ctggtggtcc tttagttaat
     4201 atcgaaggac aagttatcgg tatcaactca agtaagatcg caagtacttc tgaatctact
     4261 tctaatgtaa gcgtagaagg aatgggattt gctattccaa gtaacgatgt agtgaatatc
     4321 atcaatcaac tggaaaaaga cggaaaagtg acacgtccag ctctaggtat cacaatggtc
     4381 gacttatctg ccgtttctac tcagcaacaa gaacaaatct tgaagattcc ttcatctgta
     4441 actaacggtg tcatcgtgac aaatgtccaa acagctacac cggctgaaaa agctggattg
     4501 aaacaatacg acgtcattac taagatcgat gacaaagaag tttcttctgg tgtagaactt
     4561 caatctgctt tgtataagaa aaaagtggga gattctgtca aaatcactta ctatgaaggc
     4621 tcgaaagaaa aaacaactac tgttgaattg acgatcgatc aatcggcatt aaaacaaaat
     4681 caaaatagtg aaaattaata aaaaagaggt tgtgacaaag acttgtcaca acctctttat
     4741 tttggaaatg cttctccgac tacggcatcc tcaggcaata ctaagattcc tttttttgct
     4801 ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca ttccatagct atctacccct
     4861 cgcaaagttc ctggccagat catcattcca tctggcatca tagctcctgg ttttgcaacg
     4921 acaacttttt gtccagcttc gatattgggt gcaccgcaaa cgatttgcaa tactgtacca
     4981 ttgtctactt ctgtctcagt aatcgataaa tggtcagaat caggatgtgg gacgcatgtt
     5041 tttacgtacc cgacaacgat cttcggttca tgatcagctt caagctgttc ttcgaatcct
     5101 gcttctttga tcaatcgatt caatttcgct aattcctcat ctgttaagct tacttggccg
     5161 ttcccttcga ttgttagatg atcggagata tggaaaaagt tccaagcaac aacttgacct
     5221 tgttcatctt tgatccgagc aacattccat ttgcgttcaa cagtgttttc tgttccttta
     5281 tcgtcggcta taataaccat caaggtgtct ccgacgtgtg ctttgttata agcaaaaatc
     5341 atgttctttc ctccattatc ctttgctgag atttatctta acaaaaaatt gcattcactg
     5401 catgaaattt ttttcatgct ttatttccga agaagcctct tctggaacac catttattcg
     5461 taacaaggga ctgtgacaag acttttgtca cagtcccttt actgttttag ttaattgatt
     5521 aaaaaggtcg tacaagttcc acttgcttaa ttacttggct gttcttcttc gtcagaaacg
     5581 atcaagacat cacaaggtga atgacgtatc acgtagctgg ctacacttcc tgtcataaag
     5641 cgttcgactg catttagacc tgactgccca accatgatta gatcgatctc atattttttt
     5701 ggcaatttga ctgccatgga ttcacgaaca gaaccatatg caataattcc ttctatattt
     5761 gtgtaatcaa cagatttccc atattcttta cattcttcga tcatttcctt ggctgtttca
     5821 gtttgctgat cgactagatt ttgatcaata ggtgcatagc ccataaagtt ataaaattgc
     5881 tgatcgatta cgtttgctac atatactttc ccattattac ggcgagccac ttctaccgct
     5941 tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac ctactaaaat attcttatat
     6001 tcttgattca tcttgacccc tcctaacaga tgtcagcctt ttgttacact ttctaggaac
     6061 gattcgattt cctgttttgt tttacgatct ttattcacta gacgacctaa ttcttttccg
     6121 tcttggatca cgacaaaact tgggatacca aaaatgttcc attcagcggc tacatctatg
     6181 tacttgtcac ggtctacttc aataaattgc caatcagaaa agtcattttc aatttcaggc
     6241 atttgcggtt tgataaaacg gcagtctccg caccagccgg cagtaaagaa aaatacactc
     6301 tttccagttt ctacgtagcc tgctaattct tctaaactat ttgggataat cattctttgt
     6361 cctctacttt ctgtttatgt atttttcaat taaattatac acctttcttt cgaactaaca
     6421 aacattgatc ctctttttat aaaaaaaatc tggcaaaaag caatcttttg ccagatttta
     6481 tagtttcact atcgcattat tttccgtata cgatcgtatt tactgttgat ttatccaaac
     6541 ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa atcgcgaata ctgaacatcg
     6601 tttgatgcgt atggatataa cgtcccacta cgccaatcac tgtacttggg attccttcgt
     6661 tttgtgtatg agcagcacct gcatctgtac cgccttttga aacaaagtat tgataaggga
     6721 tattgtttgt ttcagcgata tccagcaagt actcgcgcaa acgaggaagc atgatcaaac
     6781 ctggatcttg gatacgcatc aatgtacctt ctcctaaatg tccgaatgtg ccatttttcg
     6841 tcactgtatc atctgctgca gagcagtcta ctgcaaagaa cagatctggc ttgaatttat
     6901 tgacagaagc tttagaacca cgcaagccta cttcttcctg gacattcgcg cctgcaatca
     6961 atgtatgacc taatgtttca ttttgcaacg cttctaaagc ttctaatacc attgtacagc
     7021 catagcggtt gtcccatgat ttactgataa tattctttcc gttggctgtt ttgatcgttt
     7081 cagcgtaagg aacgattgtg tctcctggaa ggacaccgta actcattgcc tcttctcttg
     7141 attcaaaccc agcatcgaac aaaatatcgg ttacttctac tgatttttgt ccgcttgttc
     7201 ctcgcaataa atgtggggga acggaagaag aaatacatgg ataattctta ccagtagagg
     7261 ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt ccagccgcct agaggtacga
     7321 ctttaaaaag cccgttttct tggatttgtg tcagcatgaa tccgacttca tccatatgtg
     7381 ctgcgaccat tacacggggt gcatccgctt cttcatgatg gcgcagacca aaaatgccgc
     7441 ccaaaccatc taactgtaac tcatctacca gcggttccat atgactttcc atataagcgc
     7501 gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt taactctttg attcgttgaa
     7561 atgttttttc ttccatatgc tattcgtcta aatttcagac taaactcctc tcagacttat
     7621 attgatctgc ggataaaata tgattttttc tgcagaccga tcgtatttat tatagcttat
     7681 tttttctggt agcggttatt tatttcaaag aaaatatgag agtgtggtaa aataagcaca
     7741 aggagtaaca agtgagaaga aggaggtatg tccacatgta tgaagaaagc gaactaagct
     7801 attttaaagg cgggttagca gttggaatcg gtctaggatt attgagcggg gtagcttcaa
     7861 cgatttggta tcatcgcaaa aaagtattgt cagcagacca agtacttgaa aatatcaagt
     7921 catcttttct aaaagaaggt gcaatcgaag gttcttggat ctcttttgaa aaggaaccta
     7981 ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg tctttctcga atagaagatg
     8041 gcgaaactgt ttattatgaa tttttagcag atgctcatac aggaaccgta ttagaaattt
     8101 caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt gagagaacga atacgcaccc
     8161 actattcaaa agagaggtat catgtccttc ttttgtagtg ggtggttttt tagatagagc
     8221 tttccaaaca tatattccag tattttcttt ttaaataaat gatatttagc gggaaaaact
     8281 agcatttcct ttgattatga ggtattttta atttagcttt tgttttctat agttaaggag
     8341 agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat tccttgttac aatgaggaag
     8401 caacaatttc aacagtcatt gcagacttta aacgagaatt accagaagca gatatttatg
     8461 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggt ggagcaatcg
     8521 taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg ccaaatgttc tttgatatcg
     8581 atgccgacta ttatttaatg gtagatggcg atgatactta tcctgcagaa gctgtacatg
     8641 gtttgctcga gaaattacgt tctggtgaag cagatatggt cattggtgat cgcctatcaa
     8701 atggaaccta ctttgatgag aataagagac ctttccacga ttttggaaat aaccttgtta
     8761 gaaatacaat tactagaatg tataaaacaa aaattcttga tgtcatgacc ggctatcgag
     8821 gattcaatcg tatctttgtc aaaagttttc ctattatgag ttctggtttt caaatcgaaa
     8881 cggaattgac gatccatgct ttagataaaa aattcaagtt cgttgaaatc ccaatcgatt
     8941 atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac attctctgac ggattcaaag
     9001 tgatcatgat gattattaaa atgtttaaag attataagcc tttgcttttc tttgggattt
     9061 ggaccatctt cttcttttta tttggattgg tcacggggat tcctgtgatt cgcgaatata
     9121 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
     9181 tagcacttct ttcattagta acagggttga ttttggatac agtcgtcacg aacgctaaga
     9241 aagaatatga attgaatcta tatcatgttt atcatgaata ccacagtaga aagtaaaaaa
     9301 aggatcaaga ctgcaaaaat aatttgcaat tcttgatcct ctttttaatt aattatttcg
     9361 ccaaacgata gatggcatct gcatagattg ctgttgctct gaacaagtca tcaagactca
     9421 taaattcgtt tgcttgatgc attgtatcag tatatcctgg gaacatcgct ccataagcaa
     9481 cgccacgttt caacaaacgt ccgtaagttc cgccaccgat gatttgttcg tagccttttt
     9541 ctccagtgtg gtcttcatag acttgcaata atgtagccac caatggatca tccattggta
     9601 cgtaatgtgg ttccattacg cgagcacctc gagtaactgt tgcgccttct tgccccactg
     9661 tttgttcgat gtcactttgc aatccgtcta ctgttacacc ttttggaaaa cggaaattca
     9721 tattgatgaa gttcgcttcc tctgtaccat tttcttcaaa agcaaatagt ccagcattca
     9781 ttgtcaattt gcccattttt tcatcttcat aagcgactcc tagtttttct ccataaaaat
     9841 cttcatggac ataagcagct gctacatgga taaattgttt agcagatcct aggaattcat
     9901 agttatctaa gaacaatgcc aagaaagaac ctgcattgat accagattgt gggcttgcac
     9961 catgtgcgcc tttaccaact aattcgattt ttacatacgt attgtccgct tcaatcgttc
    10021 cgcttactgg atttgcttcg atgaattggt aaaatgcttc ttccatcgcg atagctgcat
    10081 ctgcacttgg tacttgaagt gctgcagttg ctgttcctgg caccatattt tcgcgaagtc
    10141 cggaaacaaa agatttcaag acataatctc ctgcattgtc gcctttgaag cgtaaagcta
    10201 aagaaacatt tcctttttca ccattgatga ttgggaattc agcatctggt gagaaaccaa
    10261 aatctggttc ttcttcatgt tcgaaataat aagccatatc gccccagccg ctttcttcat
    10321 cgctgccgac tacgaagcga actttttttg atagtttcaa gtcaagttct ttaatgatct
    10381 tcatcgcata gtaagctgcc atacttggac ctttgtcatc gcttgatccg cgtgcaaaga
    10441 tctttccatc cttgattact ggttcataag gatctgtgtc ccagccatct cctgcaggaa
    10501 cgacatccat gtgtccaaat atccctagcg tttcgtctcc ttcgccaaga tcaatatgcc
    10561 ctgcatagtt atcgacattt tttactttaa atccatcacg ttcgccataa gctaacatat
    10621 gtttcaatgc gtcgcgaggt ccaggtccaa atggtgcatc tggtgttact tttgagtcat
    10681 cgcgctcgct gttgacacgt aaaagatttt tcaagtcttc aagcaagtct tctttacgtg
    10741 cttctacttc tttttgccaa tcaattgtca taaagttaat cctccactta tttaagtctt
    10801 cgtgttcatg ataccacaaa ttacagaaaa aaatagttca aaaaatctga caattccttt
    10861 tcttatctgt ttttctctaa aaaaaatata cgctataatg agtaaaagga ggtttttaga
    10921 tgctgataaa cgaacaacta gtgaaaagag tcatcacacc tcgtccaaaa atttcacata
    10981 aaggaacatt tggacgtatc gttttgattg gcggcaatga acaatatggc ggagcaatca
    11041 ttatgagtgc tttagctgcc gtacacagcg gagcgggtct cacgactgta gtaactgatg
    11101 aaaaaaatcg gagtgctctt cacagtcatc tgccagaagc aatgattgta gtttggacgg
    11161 atactgacca gtttctttcc gtaataaagc aagcagatgt actcttgatt ggtcccggat
    11221 tgggtacttc ttctaagagt ttggaacgtt tcaaacaagt actagaaaac caatcaaaag
    11281 aacaatggct ggtgattgat ggctctgctt taagtttact tgctgctaat catcttttgc
    11341 taccctatcc cgaacaaact gtacttacac ctcatcaaat ggagtggcaa cggttgagtt
    11401 ctctaccgat acaagaacaa cgggataaag aaaacaaaaa gaagcaacag gaaatcggtg
    11461 ctgtgctcgt tttgaaaagt catgagacaa aaatttatgg tcaccagcac ttctacacca
    11521 atcctttagg ttctgctgct atggcaacgg gcggcatggg tgacacttta gctgggatga
    11581 tcacaggatt tttagcccag tttcctcaaa atgaccaaac gattgcagca gctatttatc
    11641 ttcatagttt cattggagac cagctggcaa aagaacaata tattgttttg ccgacacaaa
    11701 tcagcgaagc acttccgaaa tggatgaatt atttttgtaa aaaatcaatg gaaaacgagg
    11761 catgagtcat ggagaaattt acaggttatg tgcgagaacc tttttattat gaaacagatc
    11821 aaatggggat tattcatcac agcaattata tccgctggtt tgaagaggct cgggtagcct
    11881 tattggtaca tctagggttt ccctataaaa aaatcgaaga atctgggatc atcatccccg
    11941 ttctggaagt aaattgcagc tataaagaaa tgatccatta cggagaaaaa gtgagcatcc
    12001 atccaactat ccaaaagtat acggggacac ggttggattt tagctacgaa attgtgggaa
    12061 ctgaagacaa aaaattaaaa actactggca gtagtaaaca ctgtttttta ctttctgatt
    12121 cgcaacgatt agtgaagctg agcaaagtca atccggagtt agatcagtta ttccaagcgt
    12181 acgcttcttt ggatgatgta aattcttaat aaagaccgtc taagcatttt tcttgtattg
    12241 tgtcgagtag ttcgtataat gaaaagaaaa aagcaggtga tccccatgta tgtcgttaaa
    12301 gtaatgcacg gttacatcga taaaactggc tgtcgcactc gggaaaaaaa cttagacaat
    12361 ctgctcattt tcaaagacaa aaaagaatcc gaagcttttg ctaaaagaat cggcggtcgt
    12421 gtcaaaccga tccaagaggt acgaccagat tgattcaata tttttctttt cgaggctcta
    12481 ataggcatgc ctatcagggc tttttttgca gactgtgttt ttatgcccac acgatttgta
    12541 agcgatttat gttataatag attatatctg catagaaagg aaggttaaat ggttatcaat
    12601 aatttgcaac agcaagctta tgaatctata cgtaaacaaa tcatttattc cgagttagca
    12661 cctggaagta aaatctccga taaagtgtta gaagaacaac tagctatcgg gcgtacaccg
    12721 attcaagaag cactgatcca actgcgcaat caagagcttg taaaaaccat tccacagtcc
    12781 ggcacttatg tttcaaaaat cgatatccga tctgcttcat tagcccgtta tacgcgggaa
    12841 aagttggaaa aaccgatcct gcaagaatgc agtgcgaaaa tgaccaagca agatcaagct
    12901 aaactagaaa aaatactaga acaaaccgat caggcagtaa tcgctcaaaa taaaaaagct
    12961 ttttttgatt tggatactga ttttcaccga acttgttatg aaattgctgg aaaacgagaa
    13021 atttgggatt ggctagaaag ttatagtacg cacttgaacc gtttcagatg gttacgtttg
    13081 acgatttcag aattagactg gggtcgagta ttagatgaac atcagacaat gcttcaatca
    13141 atgattgatc ataactttga tgaagtcgga tttctttgca cgatgcattt acatatgatt
    13201 atcgaggagc aagaatacgt gattcataat taccccgatt attttgaaga tattcaggac
    13261 agacccatca aataagaggt tttggaatca gcgttttgac ctgagtatta gagaaagaaa
    13321 cggcaaaata gttcctcata ttttgccgtt ttttttgagc taataccgag ggtcagctga
    13381 ttgcacaccg tttattgaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    13441 ccaagtaaaa atagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    13501 cttttgaaca ccgttttcca agaggtcgtg agcctgccgt tcatctccga gtcacaagga
    13561 acaatttcaa aaaacagctt ctcatgtttg ttgaatattt tgacttgtga ccgaggagtt
    13621 gacaggagaa catcgtttat tcggaaaaaa aggactgtga cacgactttt gtcacagtct
    13681 ctttatttgt aatctaaatc atactccgta catctttttt ctattttttt tatttcttct
    13741 aagatccctt ggctacgaat ttcattatat ggccaaagta agactgcctc taatacataa
    13801 ggcgaaaaat cgctttgatt catctctttt gaaaaactca tcgctgctat ctcgctcaat
    13861 gcacgaatgt ttgattgatg tgtataaaaa ggcaaccgcc acagcacaat tttttcgctt
    13921 tgcgtataaa ttcccttaac agaagcttcg atgtggtgaa atagttcatg tccaataatc
    13981 aaagcttgcg ctgtttcttt agagatcgaa gctagtcctg aacattttaa tttttctaac
    14041 ggctccgtca tcaatcgaat cccttcatct tctgcataag ttgcaaaagt gattcgttcg
    14101 tttatttgct ctgattcaca ggaaacgatt ggaacagcca tttgttcaca aatttctcct
    14161 acatcggcaa aagggtactt ttttttgatg ctacatgccg tttcgatacc acaatcgatt
    14221 gattgttgta taagaatttt ctgcttggac aaaggaatct ttttccaaaa taaatctcgc
    14281 cgaaactggt agaatcccca aatttcttcg gggatctgtt gtaattcgtt tagtaactct
    14341 tctaacttat gtttgtttga tatttccggc aacgacgatc acttcttcct ccggttccat
    14401 agtagatact tccagatcca tcaaaagctg tagttgttcc agatccgtcg ctgtaaaatc
    14461 agagactctt gctcctaagc agagataaaa ttctggtcgg gcaatcaatt cagtttgata
    14521 aactgccata tcttcccaaa gttgttcgac agctgtcata taattggctg cagttgtttt
    14581 catacatgaa gcatgtccac gttggacctt gataaatcct ttttgatcga tgatccgaag
    14641 acttccagca ttttttgaac gattctgttc gccgcataca taaaagaaag gtgtattttc
    14701 caataagcaa acttcatttt tatttaatcg catatcctct gcagcaagct ctagtgcttc
    14761 ttctgttgtg atctccttgg ttagatcggt tgctttcact tccgtagaac cagtagcgat
    14821 agccgttact tttgaagtct gtggatcgat ttctacgtgg acttcgatcg attctggtgt
    14881 agcgcctgat tcaatcgctt tattcatcgc ttcatttttc aacgatcgta tatcttcttt
    14941 acttggcgaa gggatgatac gttctacaac atctcggacc attgcaagtg ctacaccaat
    15001 agaagaaatc acttcggcgt tttcaggaat cgaatatttc acacccattt tattactaaa
    15061 gtaagtaatc aatgaggcag cgccgccccc tacacctaca agagaaatct gatctttctc
    15121 caagtgatac ttgtctgcca attctaaaat caccggttca atcttagcat aggatttttc
    15181 catgatctgt tctgcgatgt cttcaaccgt cgtatgacaa taatcagcta atgcttggat
    15241 ggcttttcgt gcagatggga catttccata agaaaaatgt tcttcttgca ctaatccaag
    15301 gacattggct gcacatgtat ttgtaatagt cacttcttct ccgtcttcca tcactacctt
    15361 gacgtaatca gcagggtctc cttctttagg agagaaaaat tcaaccttag gacctttgat
    15421 tttttctgtc tccgtaaata ctgcataatc tagaccggca atatgtgcag aacgtggacc
    15481 gacatcgatg attcctgact gattagcccg taccatcgaa ccgcctgcca cacctagaac
    15541 ccgaacatct aatgaagaga tatatgttgg gtggccacct acaattgaat aatcgattgc
    15601 tggacgtcca tttttaatga ccccgatatt ggttgttgtt ccgcctactt caaaataaac
    15661 accattagac gctctcaagt acatcaaaga gcccatgact gatgctgctg gaccggaaag
    15721 catggttaaa acaggtcgtt ttttcatttc attgatttcc attactccgc cgtctccgcg
    15781 cataatcatc aaagaaacat tgacccctgc ttctcgtacg gaatcttctg tagaggttgc
    15841 cgtatctagc atttttggta aaatagatgc attgattgct gctgtccgag ttcttcttgt
    15901 caatccatat aattttgtga tgtcagaagc catcgttgta gggatcgatt ttaccgatgc
    15961 tgcttcataa acaactcgtt ctggttcccc attatctact ccaaatgcca tagaagaaac
    16021 caagacctcc gctctttctc tttctaaaga agaaatcgtt tctgaaacac ggtctacatt
    16081 taagtgtttg acaggtaaga atgcattgac aatctctatt tttttcttat tccctaaatc
    16141 gatatcgttc aatctagttt gtcgttttgc taaaaaacct tctaatccac cttttgccat
    16201 cccgattacg ccgacttttg ctacgtctcc ttcaatcagt gcattagtcg cttgtgtggt
    16261 agaatgtgca acaaacacaa catcttctgg actgatatta ttttccagca aacaattttg
    16321 aaaagactga acgacacctg cggctactcc acggacatca tcgtgggttg ttttcactga
    16381 agattttccg atgatttcgt gcgtcgcatt atcgattgcg actgctttcg tatgtgtacc
    16441 gccaacatca attcccattc gcacaaaacg tttcattttc ttttcctcca ttcatctaat
    16501 cgtttttatc gacgttttgt ttactaggcg ttcccgtaca ataagaacgt gatcaggcat
    16561 aagatcgctc cgatgatcca accagttggg atggatttct tcatatattc tcgagaagtt
    16621 acttttgtat aacctaatcc ccaagcaacc caagattgtg taatatctaa atgctgagga
    16681 gcaatcgttg tgattgcaaa taacgggaac aaaaaggctg gcgacatcgt tggattaaca
    16741 gctaatacga cagctaaaat agctgagcct gaaccgacta gattcattgg accgcggaaa
    16801 aatcctagtg gtgtcaatat agcaaatacg attgccaaga ctaatggtga ttgcggtatc
    16861 caatctccaa aaatagctga aaaataagga gaagcatacg cagctgaatt attgaacatc
    16921 gctaaggtca tcaagaaacc aaccattggc gcaacatcta ctgcgccatc tgtaaataat
    16981 ttcgcaaact gacgacagat ttctttataa cctccgcgca attttccagt aaacagcaat
    17041 gcccagatac ctgctagaat aaaacctaag atgattggaa gatctaaaag aacgacccct
    17101 aaaactggta ataatacggc taaccatgag atcattggtg cgtcgccacc ttcagctggt
    17161 acgtttgctg ccattgcata gcgtttcttt ttgttcattg atatattagc gaccgttagt
    17221 accacaacta agcttacaat catcccaatc ataccaattt ggaaatagtc attataagta
    17281 tagctttcag ccgctggatt gaaaccagca taaatcgttt gatattgttt gaaattgacg
    17341 atattagcaa agattccagc cataatcgag cccataaatg agaacagtgc aaccggtact
    17401 gaaataccta atgacatcaa gattggcaac acaataactg caatagaaat cactggtccg
    17461 attcctgtca tcgacatgaa tataacagcg gttacaatac atagtaagct catcgtgatt
    17521 cttggcttgt cccctccaag ttcaactacc tttcgaatca gggtagctgc gattccgcta
    17581 tctactagaa cacgcccgaa aaaggcacca aagaagacat ttaccaagat cgatttggcg
    17641 tactcagctg gccccgtcgt atagatatgc gtcaacacat caattactcc ttgcccttcc
    17701 atcgccgggt ttggtgaaaa cgtgtttccg actagcgcta atcccatcca tacagtagcc
    17761 atgatggcaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
    17821 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
    17881 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
    17941 gctttcatta aagggtgacc ataaatgttc agttttcaat taaaaaacct taattatgct
    18001 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
    18061 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
    18121 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
    18181 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
    18241 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
    18301 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
    18361 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
    18421 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
    18481 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
    18541 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
    18601 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
    18661 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
    18721 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
    18781 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
    18841 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
    18901 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
    18961 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
    19021 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
    19081 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
    19141 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
    19201 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
    19261 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
    19321 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
    19381 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
    19441 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatc
    19501 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
    19561 tgaaaatgat tagattagca gactttcttg cagtgattcc cgcttctttg ctatactaaa
    19621 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
    19681 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
    19741 ggggtgacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
    19801 aacttacgat gcaaaaaaca gttttacgtc attttgaatg cagtaatgtc atttttgaaa
    19861 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
    19921 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
    19981 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
    20041 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
    20101 acaatcagtt gaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
    20161 ctaatttctt cacagcaatc gctttatctc aagattcttt ggcgggacta atcgtcaacc
    20221 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
    20281 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
    20341 gattccccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
    20401 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
    20461 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
    20521 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agcgcatcgt ccaactcttc
    20581 tttttccttt tctgacaact tgcgataaat agccaatgcc ttgatttgtt ctaaaagtac
    20641 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
    20701 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
    20761 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
    20821 taaactgtat acactcatgt ctggcattcg ttccaaaagc gatcgaatta gtcgaatcac
    20881 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
    20941 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
    21001 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
    21061 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
    21121 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
    21181 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
    21241 accctttatt tgagcaactg taaatcaaat ggctactttg gtttggcgaa tctctgctaa
    21301 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
    21361 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcgta
    21421 acgtctgttc aattctttgt aataatcgta tagacttgga tcaaaggaca attgttcata
    21481 cgaacgacca cgtttttcga tacgctccaa catcgtatca aaagatacac gaatatgaac
    21541 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
    21601 cagttcatca tacacacgaa cttctgtttc tgttgctctt cctagatctg catttagatg
    21661 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
    21721 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
    21781 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
    21841 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
    21901 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
    21961 taactccaac ctttttaaat atacccccaa tatctagtag tgtcaattgt atttttttct
    22021 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
    22081 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
    22141 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
    22201 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
    22261 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
    22321 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
    22381 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
    22441 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
    22501 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
    22561 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
    22621 ctattagaaa atgtccagct ttcggaaaaa atcgcccatg gtaatcctag tggaatcgat
    22681 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc tttcgactac
    22741 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
    22801 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
    22861 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgaaaattca
    22921 ccagaaacgt tggcacagac aatggatgaa tcacaatcac ttctggaaaa gctgacaatc
    22981 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
    23041 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
    23101 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
    23161 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
    23221 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
    23281 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
    23341 tgaattttat ctagatggca ccttgcaaga cgaaaaagca acaaaaaaag tcagtcagtt
    23401 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
    23461 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
    23521 cgcttgcaat actgctctta agcttggatt agatgatctc tctctttcaa gatttgctcg
    23581 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgtcgaat gggaaaaagg
    23641 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
    23701 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
    23761 gcgtcggaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
    23821 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
    23881 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
    23941 ctggtctcct gacagcttaa aagcgatgga tgcggtgcga caattgagaa agcaaggcat
    24001 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
    24061 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
    24121 acatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
    24181 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gccatccagc
    24241 agttatcgct gcagtcgatc aattcgtgac agtcactgta gaatctgcac gaaaagtcgg
    24301 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
    24361 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgcttgac
    24421 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
    24481 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
    24541 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcagt tggagatttt
    24601 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
    24661 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
    24721 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
    24781 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
    24841 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagataaaa tggtggctta
    24901 tcagcttttc ttaaaaaata gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
    24961 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
    25021 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
    25081 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt gggggcgatt gcggaatcgt
    25141 aattgttgac cagaaatctg gcattcttcc tttaatgagt gcatgggaaa aagcagaaat
    25201 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
    25261 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
    25321 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
    25381 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
    25441 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
    25501 attgccactg gttcagtcag cgctgccttg aaagtccctt ctttagccga tacttatacg
    25561 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
    25621 gtcgaaagag ccaaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
    25681 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
    25741 cttattcaag aaactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
    25801 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
    25861 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
    25921 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
    25981 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
    26041 aaagcccttt gtctcggcgc aaatgccgtg ggtacttcag gaaccgtact gacgcatctg
    26101 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
    26161 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atctctgatc
    26221 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
    26281 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
    26341 acgtacaaac ggcggaagta aaagcaactc cttcggaaat aagctaaaat tcacaaaaat
    26401 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaatgct tctgtcccag
    26461 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaatccgct
    26521 gatttcacaa cctctccact aaacagtgta ctaaagctct tttaaggttg ttttccgata
    26581 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
    26641 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
    26701 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
    26761 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
    26821 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
    26881 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
    26941 ttaccgccgc ctttttcgat catatccgga ataactgctt ttgaaacgat gaatggggca
    27001 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
    27061 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
    27121 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
    27181 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
    27241 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
    27301 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
    27361 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    27421 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    27481 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta caactaaatg
    27541 ttttgtttca tctggtttgc ccatcatgtg gaacacacgt gtatcttcat tggcataatc
    27601 aaagtagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    27661 gcttcctggt tctaaaatcg tataacccat ttgtaattga cagctttcgc atacatttgg
    27721 atggatgtat tggtagattt tacgttcatt cagtgtcagg ccttcaccag tctccattgg
    27781 ttttacttgg tcgatactga tttttacatt tggatattta tgatgagctg gaacagaact
    27841 aatatagaat ttagctgggt tttcagcatc ttgagaagaa aatacaacat gtcgtgtttc
    27901 ttttccgata tagtatccgt cttgtttttt catttcttct ttttgcccat cgatctcgat
    27961 gaatcctggt ccaccgatat tgatcacacc tagttcacga cgctctaaga agtaatcaac
    28021 acctagttct ttgtctaatg tgatttctaa ttctttttca gtaggtgtca ctccaccaaa
    28081 gatcattcga tcgttgtgcg tataagtcaa actgattttc cctggttcaa ataccttttc
    28141 tactaaaaat tcttttctca attgttccgt tgaataatga cggatatctt ctggactgtg
    28201 tgtataacgt gtttccattt cttgcatttt cttctactcc ttctatcgaa tgatttttcc
    28261 agagccgcat gctaaaaatt gtgcgacttc ttcttgacta aattggttgc agtccccatg
    28321 gatcgtatgc ttcaaggcag aagctgccgt agcaaaatca atgatttctt gcggagacat
    28381 attggataaa atcccatgga gcacgccacc tgcaaaagca tctccgccac ctactcgatc
    28441 cacgattggg gtgattctat ggacttggga ttccacatat tcatggttca tccacaatgt
    28501 ccccttcaat tcattttcac ttgctgaatg aacggtacgc ttggtcgaat aaaacacctg
    28561 aatattcggg aacttttcct gcattttttg ataataataa attagaggtt gttcttctgc
    28621 ttctggcgct ttctcgatac ctaacaaata aagagcatcc atttttccag ctgagcagat
    28681 atctactaaa ggtaaaagct gatggattac ttcgctggct tcttgctgtg tccataattt
    28741 tccacgatag ttgatatcga agctgatcag gcagccagct ttttttgcag cttctatcac
    28801 ctgtttcgtc attttttgcc attttttaga caaagctggg gtgattcctg aaacatgaaa
    28861 tatatctatt ccttggaata actcctctag cgaccattcc aaatgatcca tttcagcaaa
    28921 acttgatccg gcacgatcat agatcactga ggcggcgcgt tcgccactac cagtttccat
    28981 ataataagta cctagtcttg atccaccaga taatacctgt ttcgtatcta ctccataaca
    29041 ttgcaagtgc tttttgactg cttttcccaa tgcgttatca ggtacttttg tagcaaaaat
    29101 cgcttcatgt ccgtaattag ctaaagaaat tgctacgttt gcttcccctc cgccataatg
    29161 ggcagcgaat cgttcacttg aatcgatacg ggttccctgc tctgtggaca atcgcaacat
    29221 gatttctccg agtgtcacta cttttcccat aagcagctac ctcttgattt ctttcatttt
    29281 tgctgcatac tgttgcgcga cttccgttac tttgtcaaaa tctcctgtag ctgcgggtgc
    29341 taaaaggttc ccacctactc cgactgttac gacaccagca tcgaaccact ctttcatatt
    29401 gtccagactc acaccgccag ttggcatgat attgacttga ggcatcggcg ccttgaaggc
    29461 agaaatgata cttggtccat aagcgcttcc aggaaatagt ttgacgatat ctaccccgct
    29521 tttcagcgct gttttgatct cggtaatagt catacatcct ggtaagtaag gaatttgata
    29581 cagattgcac atctctgccg tttcttgatc aaaggaaggg ctaacaatat actctgcgcc
    29641 agctaagata gccaagcgag ctgtgacagc gtctaatact gtgcctgcgc caatcactat
    29701 ttctggttgg tctttgtaga atgacaaaag ttctttgatt acttgatctg cttgagggac
    29761 tgtaaaagtc aactcaatac cagttagacc acctttgatg attgcgtggc aagcattcaa
    29821 agcttcttct ttgctttttc cgcgtaccac cgcaatcact ccggcgtttt tcaatcgaga
    29881 taaaatatca atttttttca tcttctccac ctatttccta tttagaaatt ttattttata
    29941 acttggaaat ctatacccat attatacggc agaaaagaaa aattgcaaac gcttttttca
    30001 ttatttttga attctttgct cgatttcgct ttttgtttgc ttgagggcac tgattatttg
    30061 ttcttttgtc tcatctgtta aacgatattt cggcatactt acactaaatg caccaatgat
    30121 ttgttcgttt ttcacgattg aggctcctat acaaaaaata tcacgttcga tttcttcatc
    30181 gtcaaatgcg atgtgggttt cttttaccgt tttgatttcc tttttcaacc gtaatgcatt
    30241 ggtgatcgta tattctgtgt aagggatcaa cggatgcttt tcaagataat cagtgtactg
    30301 ctcttcactg aattctgcta atacggcttt gcccatcgca gaattgtata atggtcgggt
    30361 gattccgatt tttgaagaca tgcggatcgt ttgatgtttt ggatctagtt tgttcacgta
    30421 caagatctca ttgttgtcca atacccctaa gtggatagtc tcatcgatct tttgctgcag
    30481 ctgttctaaa aacggtaaag tcaactctac caaatcgatt tgttcaatgt ttttattcgc
    30541 gtagcgaata agctttgcac ctaaacgata gtttttctct gaatttttat ttacgtagcc
    30601 gatcatcaac aaagtatcta gtattttcaa gactgtagaa gaggtcattc ccacaccctt
    30661 tgcgatcgtc tgcaaagaga catcttggtt ttccgctaaa tagtctagta ttttagaggc
    30721 tttgatcaat acagttccat aaggcttttt attttccata taatattctc ctatttcttt
    30781 ataagaaaaa ttatagcgga aataccatca atttaaaaga gatatccaga tataaactac
    30841 catttctctt tacaggcaat ttaatgttga agatccgctt cgtttttaca tcaaactttg
    30901 atataaagca atgatttctt ctactgtcgt ttctcttgga tttcctggtg tgcatacatc
    30961 tcgcaaagca tcttctgcaa ttgctggaat atctgcttct tttacaccta attcagaaat
    31021 ggtttctggg attccaaccg ctttgctcaa ccgatcgatc tcaccacaag ccgcttctct
    31081 tacatcttct aaagacattt cagctgctcc tttaatccct agaacaagag cgatctcacg
    31141 gtacttttct ccagtatatt ctttgttata tttcatgaca gttggcaaaa gtgctgcaca
    31201 cgctacgcca tgaggaatgt tgtaccatgc acttaacggg tgagccatcc cgtgtaccag
    31261 tcctaaaccg acatttgaga agcccattcc cgcaatatac tgtcctaatg ccattttttc
    31321 tcgcccattt tgatcgccat ctacagaagc ttccaaagaa tggccgatga tttcgattgc
    31381 ttttaaatgc agcatatcag tcatttccca agcgcctttt gtgataaacc cttcaatagc
    31441 atgcgtcata gcatccatac ctgttgatgc agccaatttt ttcggcattc ccatcatcat
    31501 atcgctgtcg atgaacgcga cgattgggat atcatgagga tcaacacaaa caaattttcg
    31561 gtgatttact tcatctgtga tgacgtagtt gatcgttact tctgctgctg ttccagaagt
    31621 cgttggtata gctaatatag gtaaacatgg atttttcgta tcagcaacac cttctaaact
    31681 aatgacgtct gaaaactctg gattcgtcaa aataatccca atggcttttg ccgtatcgat
    31741 tggcgaacct ccaccgattg cgatcaaaca gtctgcctct gcctctttaa caaaagccac
    31801 tccatctttt acattttgga tcgttggatt aggtacgatc ccgtcgtata aagcataagc
    31861 gattgctgct ttatctagaa gttctgttac ttttgttgct ataccgtgct cgatcaatcc
    31921 tttgtcagta atgacagctg ctttgccgaa tcctcgctgc tgaaattcag ggacgatcga
    31981 ctcaatcgct ccttttccaa aatacgatgt ttcatttaaa atcattcgat tactcatttt
    32041 ctattcctcc taatttgtat tatttttcac aataaaaacg aaagaacccc aagcaaccgg
    32101 ttgcccagtt gcttgttgct acagttcaaa gactggcatc aattcccaac tgactggaga
    32161 ggcatctgga tttgtctcca tgatatcttc catgtacttc caccattttt gattgataga
    32221 tgtatttgcc attttttccc ataacttttc atcttcgatc actaagtaac caaataacaa
    32281 acttgtactc ggatcaagat agatcgaata gtcgatgacc ccgtgttcac gaagtgctgt
    32341 acgcatttct ggccaaagtt catcatgtct ttttttgtat tcttcttgtt tttctgggta
    32401 aactttcatg caaaaagctt ttttcaccat gtcagacacc tgctttcgta tgaacttcct
    32461 ccaaataacc tgcttttgga gtgacaccaa acgcatctgc taaatgccac aggttttcgt
    32521 ccgtgattgt ttgcaagatt ggtccttgcg ctttgacgta cgtatagact tctgctgcct
    32581 tttctgctgt ctcgatcaaa ccaaagactt catccatgtc ttttcctgac ccgtagattc
    32641 catgttgtgg ccataaaacc aatcgagttt ctttcatttt ttcagctgtt gcttccccaa
    32701 tctcatttgt tcccggaacc atccacggaa tgatggaaat tccttctgga aaaacaacta
    32761 agcattccgt acacatttgc catagcgtac gactaaagga acgttcgtct aattcatgcg
    32821 taaaactcat cgctaataga tgactagcat ggcaatgcat aacgatccga ttatctgggt
    32881 ctacttctaa acgggcgata tgactcatga aatgacttgg caactcgctt gtaggaaggc
    32941 attcgttttc cagcccccac aaaagttcca gtgttttgcc atcttcagca actcgaacga
    33001 ttccaagatt ctcggcaggt tcgctcgcta catttttaaa atatcttcct gaaccagtta
    33061 caataaaata gcatcctgct aaacggctgg catcaaaaat catcgagatg cggcggatca
    33121 ctttttcagg atccaagaac ggtgtgatct cttcttcttt tagtaaataa gaaatattcc
    33181 cgccatttct ttcatcccat cccaagcgat aaagattcga cgtcgtttcg atcatttctt
    33241 tcacataagg cgcttgtaaa atatctattt ttttcaatat agccacactc ctattttctt
    33301 aattccaaca cgtctttttg atattcctgg acttcttcca gccaatccaa accaactgga
    33361 acttgatttt tgtaacagaa ataattccat acatcggcat acgggaaatc tttcaattct
    33421 tccgtataag ccattcttga agtaaagtca aaagccagct ccatttcttt caatcgctca
    33481 actggttcca gcatagcttt cagcagtgct ttttgtgtat ttctcgtacc gatgacccaa
    33541 gcagcgatac ggttgatcgt tgcatcaaag aaatccagac cgatatttgt tttttccaac
    33601 aattcgttac ggactaattc tttagcgatt tcctgcaatt catcatccat caaaaccaca
    33661 tggtctgaat cccaacgcac tggacgggaa acatgcaaca tgattccttt gctgaataaa
    33721 gctaatgaag ataatttatt ggaaatcact tctgttggat gaaaatgtcc agcatctaga
    33781 caaacaagtt tgtcacgagt caacccgtaa cccatataaa attcatggga accgactgta
    33841 taagcttcag cgcctaaacc aaatagtttg ctttctactg catcttgtac ataccgttca
    33901 tctaactctt ctgtaaaaat ttcatcgagt gattccatta aacgttcccg tggggtaaaa
    33961 cgatcaatcg gattatcttt catcccatca ggtacccaaa agttattgat acttgtttgc
    34021 ccagtttctt tcccaaagta ctccgcgatt ttgcggcttc ttttcccgtg ttcgatccag
    34081 tattcacgaa cttctttatc tggatgagcc aatgtaaagc cgtctttcat cattggatgc
    34141 gaaaagaaag tcggattgaa gtcaagaccg atttcttctt tttttgccca ctctacccat
    34201 ttttcaaaat gtttcggctc aatttcattt agatcgacag attcttctgt atccaaatag
    34261 atagcatgaa ggttcaattt gtgtttgccc ggtatcaacg aataagcctt ctccagatct
    34321 tgtctaagct cttcaggtgt tcgtgcggct cctggaaaat tccctgtcgc catgattcca
    34381 ccggtcaatt cgccatccgg tgtcaagaaa cctttgacat catctccttg ccagcaatgc
    34441 atagaaattt tgatgtcctg tatcttttct agtacagcat ctgtatctac accgatagtt
    34501 gcatattttt cttttgcttg ttcgtaattt ctttcaatcg tgttcatacg attactcctt
    34561 tgattttatt cacgtaccat tccatcgtgt tttgcttatt ttcatctggg taaaaagttt
    34621 cacatggaaa cgactgtttg attacatttc ttgcttgttc tatattcgaa aactgacctg
    34681 tactgagcat ttgcatggca acattcccga ttgctgtggc ttcactaggt cctgcttcta
    34741 caatgattcc ggcataatct gctgtcaatt gattcagtag acgattattc gatccgcccc
    34801 ctacgatgtg cagcttttcc aatttcgttc cagttaagtg ttctactttg atcagctctt
    34861 gtgcgtaaca aagagcaaga ttatcataca cacaacgagc gatagctccc gatgagacag
    34921 ggactggctg ctctgtttca aaacagtatt gctgaatctc ttctatcata tttgggggat
    34981 tcaaaaatcg agggtcattg atttccatca cttgctgcag cggtttttct tttgcagcaa
    35041 gctctgccaa ttccgcataa ctgtactcgt actcttgcat tcgggcaact tcctggatca
    35101 accacatccc catgatgttt ttcaaaaaac gcaccgtgtt atgggcaccc cattcattcg
    35161 tataattttc agaaaaagca ttgcgagaaa cattggcgat ctttgtttca atccctaaaa
    35221 gcgaccatgt cccactgctg atataacccc aacgttctcc ttgacctggc gttcctaata
    35281 cagctgacgc cgtatcatga cttgctactg tgataaaggt cacatctggc aaatcatatt
    35341 ttgaaaattg ttctttttgc agatctccta gtatcgttcc cggctctgtc aatggtggaa
    35401 ataattcctt cgagacaccg attttttcaa gaagttctat ttcccattct tttgtccctg
    35461 cattcagcag ctgcatagta gatgcattag ttttctccgt caccatgtta ccagtaaaga
    35521 cataccctaa ataatcaggt atcagtaaca atttatgtgc ttttgccaaa ttttcttttt
    35581 cttcagcaaa caattggaac aaggtattga aaggctggat ctgaatccct gtctgctcat
    35641 acaacttatc tagagaatag atttcagaaa actgttctac tgctccattg gtacgcttgt
    35701 cccgataagc aatcggatca tttatccgat ccccttgttc atcaatcaga cagtaatcga
    35761 ctgcccatgt atcgatcccc gcaaaaaggc gatcaatccc tgtctgcttt aatttttcaa
    35821 atccaatcaa aatctctttg ataagataat cgatcttcca gcgatcgtga ccgtctttgt
    35881 tccgaaagcc gtttttgaac cggtgtactt cttccaagat caaagaacct tttgcatccc
    35941 tcgtactttt cattagtcgt ccgcttgatg caccgatatc gatggcaaca aatgttggat
    36001 tcatttctcc acctctttta ctttattgtt gattagcact ttattaataa agcgttatca
    36061 tttattgaca atgctatcat aatacagaat cttatatttt gcttcacact attttggtat
    36121 ataattgcat tatcttgaca agttgggagt gtttacatgg attactggga aaaagccaac
    36181 cactcatggt cggaagactc aaaaagagtg atcttgacgc ctacacaaag aagcaggaat
    36241 ttattttttt atatccaaga aatcggttac ttcaaagcac caatccctta ctatactgaa
    36301 cgagcacatt taccttctta cttgataaag tttactttgt ctggcaaagg ttcgcttaca
    36361 tataatgata atcagcaaca gctaaaagct ggagacttat tttttattga ctgccaagaa
    36421 tatcagcgtt atcaaacgat cagcgaagaa ccatgggaaa tggattggat ccatttgaat
    36481 ggtcagaacg tccaatcatt ttatcaggag ttcacaaaag acggccataa caccttccat
    36541 tcaacaggag atccatcaga aaacaagatc caccatttga tcacagaatt gatccacatg
    36601 caacaaggca cgaatgcccg taccgatttc cagtcctctg tagctatcca cgagttgctg
    36661 aatgaactga ttttgcaaaa gtatcagcta gatttcgaag aagaggatat tcccgcctac
    36721 gtttacaaac tgaagcaata cttagacgaa catttcaaag aaacgattac acttgatcaa
    36781 ttagaacaac aattcttatt gaataaatat cagctgagca aagatttttc caaatttatc
    36841 ggttctccac caatcgatta tttgatcagc aaaaaacttt cttacgctaa agaccttctt
    36901 cggtatacag aatttacggt tcgaatgatt tccgaagaaa tagggattga gaattttgct
    36961 tactttagcc gcctgttcaa agcaaaaaca ggactatcgc cgagagactt tcgtaaattc
    37021 agctgattca tataatatgt acgtcatttt ttcaaaatat tttgaaatat atagataaaa
    37081 ataaaataac cgcagtttct gcttctatgt aaacaacagc agctactgcg gttatttttt
    37141 agtgatcatt ctcattgttc atcttcaaaa tgttcataat gaaccaactg tttctgtgta
    37201 tcacgtttca aaaattcttt aggcgttatg ccgaactctc tcttgaatgc tttcagaaaa
    37261 tgcggataag aagaaaaacc acttttacta tatacagcac tagagctgcg gtattctcgc
    37321 aacaagtgct tggcatagat cagctttttc ttcattacat actgatgaaa cgtaaatcca
    37381 gtgtactttt taaattctcg tgtgatatgg tatttgctga caaaaaactg cttttccatt
    37441 tcctctaaag aaagcggatg agataaattt tttgagatat attgtaacat ttgttccaca
    37501 cgctcattag gaactacagc attctgttct tgcgcactct cttctttttg tacaacttga
    37561 ttgagaaaaa tcaagtattc taccagagct tgttcatatt ttatgtcttc accgtaaatt
    37621 tttttatcag gaggatggtc gataaatgat aatttttgct tcaatacttc ctgttctatt
    37681 tgcaatacac gactcctctt ctctcccatt ggttgaaaac aagccgatag attagaccat
    37741 ggcgtggaac gactttctag aaaagaaggg gtgacaaaga tatagacacg ctcgaataca
    37801 tctgaagatt ggcggacgat acgatgcaaa tcattggaat ggatgagcag cacacttcct
    37861 gctttgatcg taaattgtcg tccgttcaaa taaaaagtcg cttctccctt taatgtagca
    37921 tggatctcat agaaatcatg atgatgatag atcgttttat tttggctgac gacatctttg
    37981 acatgataga tttcatactg atcagaaacc atcgtattga cttgttcacc gctgattttc
    38041 ttttggagca tgtgccgcac ctccttttgt tcacttatgc acaattttat tataccacgt
    38101 tttttgtaat caaaaagagc tgcttttata tactcttata aaaaagaaga gacgtggcat
    38161 cttctagccc cgccccctcc attctaaata ttagttaacc aacgtcttct ttacattatt
    38221 caagaagact tcttccataa cgaatcccat catcttttgt ttcttcaaga accacataca
    38281 ggtacggacg attcttcatg atgatttctt tgatttttgt gtactcgatc acaccgtctc
    38341 ccaaatttac cggcacgatt ttttcctgtt cgacacgaaa atctttcaaa tgaaccgcac
    38401 atatcttttc accaaaacgt tcaaaagctt cttctaccaa ttggacttgt tcttgataat
    38461 cactacttgt gatcaaattg gtcggatcta aaatgattcc tagatagtcc gaatctactg
    38521 cttggatcag ctgctctacc cttgctaaag aatacaccgg atgattcaaa cctggttcta
    38581 tccccaccat catttggtat ttttctccag cttttaccaa gcgacggata acggacacag
    38641 cttcagcaaa tgcttcatct gtgaaatttt cttccgtata ttggatttcc ggcaaaacac
    38701 atccagtttc cgaagcaacc atcgacgcac caaaggaaga agcatagcga agatactgct
    38761 cgaactttgt cagcaactct tgtcgtatcg ttaaatctgg atgaatcata ttgatatagc
    38821 aacttaaaat tcctacggtc acttcttgct tttctaaaac tcgtttgaca taatttccca
    38881 tcccagaatt gatctcattt gcgccagaag gtatttcagg aaacgaaaga cctaaggcga
    38941 gctgcagtgt atgaatccct tgttcttttg ttttttctgc aagatcttct atcgtttgga
    39001 cttcggaaag atcatgtcct ctaattgtaa tgttcaacat aactattctc ctaactcagc
    39061 atattgggta gccacatgac gatttgcggg aagtaagtaa tcaaaaacaa gacgactaag
    39121 ataaccccat aaaaaggaag aagcggtttc agcattgcct ctgcttttac gcctcctaca
    39181 cttgcaccga catatagtcc tgtacctact ggcggcgtga tggagcctac acacagattc
    39241 atgattgaaa acaacccgta atgtacagga tcaaccccta cacttgtgac gattggtaaa
    39301 aatattggtg taaaaatcaa gatagcaggc gccacatcca taaacatacc caccagtaat
    39361 aacacaagat tgactaataa cagaataata aatttattat ccgttacgcc cataatcaac
    39421 gaactaattg cctgaggaat accggtaaac gccatggcga aagacatcat ggaagaagta
    39481 gccaataaga acatgatcgt ccctgacatt gctactgatt gaaacaacat attaggtata
    39541 tcttttagtt ttaccgtttt atagtaaaac atagcaatca acagggagta aatgacagct
    39601 atcgctgaag cttctactgc tgtaaatatc cccgtcaaga taccaccgat aatgatgaca
    39661 atcaatagaa ggctaggaat cgcctctttt attatttttc cagcatgagc catctgtcct
    39721 ttttcgacaa cagggtattt attcttttta gcaacgatga atgctactag catgattcct
    39781 agcgcccaca aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca
    39841 ccagcaacaa gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga
    39901 gctgacgcga tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga
    39961 gggatcaaca ctccgccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca
    40021 aataaagcat tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac
    40081 aacatcgcaa aatccactag tttcttagct atccctccat tattcatgat aatgcccgag
    40141 agaacgaaaa acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga
    40201 gctgaactaa aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag
    40261 aatgcaatac ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat
    40321 aacactaatc cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt
    40381 gctggcttgt tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc
    40441 cccgatacag gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc
    40501 tgccccattg tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat
    40561 accagaaata aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt
    40621 tcacttaaaa ccgttacggc taaatgtgct ttttgtccca caacatacgc ggaagacaac
    40681 atggcaaacc agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct
    40741 aaaaccgttc tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca
    40801 gctcccagga tctctaatac cttgtttaaa atcattttca tcacagctac gctccttgct
    40861 cttggatctt ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg
    40921 gggcaactgc ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt
    40981 cttgtgctaa agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg
    41041 ttgattcttt cgctgcttct tcaatggctt tttcctgctc atcagttagc tgttctttga
    41101 tggcatcatt catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt
    41161 tagccacacc accatgtcca gctgtataca agacaaattc attgttttct gatccgttga
    41221 tcaaattgga ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc
    41281 ctaaaagctc taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct
    41341 tcaaatcttc cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat
    41401 cataataggt caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta
    41461 tttcttcatt atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaactg
    41521 aaaaaatcga ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat
    41581 ctactgctcc tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg
    41641 ggaaatactg gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat
    41701 tcattgcatc aattactggc tggctgttgc ttgcataagc aaagcgcaag gtaactatct
    41761 catttacatc tgcatctgcg acagttgcca cactagaatt atttccgcag ccgcttagac
    41821 ctagcagacc taggctaagc gaacttgctg caaacagcag tgatgttttt ttcatatggg
    41881 tttcctttct tttaacaata agagacgagc aattctgtca aacaaagcac cgttaacgac
    41941 tgtccataag gcattgctgt gattccgata ttccgataaa aatctaaact gtcccccatt
    42001 cctgtcccga ttgaaacgtt ttgaacttct cctttttcat cgatttgttc aagcaatcct
    42061 tggatagcac gataagcgac ttctttatat tcttgtggta aataacgttt atggaccgct
    42121 ttcaaaatac cgtaagcaaa tcctgcagtt gcagaagatt ctacgtaaga agaaggatca
    42181 tccaacaacg tatgccataa accgctttca gactgataag tttttaatgc acgcacttgt
    42241 gcttcaagtg tttcgatcaa taatttgcgc aatgcatctt ctttcgataa ttctaagatt
    42301 tcaatgatct ctggaatagc aatcgtcaac cagcagtttc cacgagccca aaatgcttcg
    42361 gcatagttat gatttccttc aaacgtccag ccgtgatacc acaatcctgt tcgtttatcc
    42421 atcaagtatt tgatatggat cataaactgg taacgagctt cttctaaata ttctgggcga
    42481 ttcaacagtt tcccgatttt tgccaaagga agcactgtca tcatcaatgt atcgtcccac
    42541 aactgattct tattttccgg tccatatgtg gcatgctgta acccgtcttc atttgtccga
    42601 ggcatgtcat tcatgaccca ttctgcccat tgttctaaat aaggcaaata gcgtgaatct
    42661 ttcgtgtctt catacaagta agccatagtc aatagcggtg ccattgtatt gacgtttttg
    42721 ggcggagcgc cttcttccat acgatcctca aaccattcat tgaccacttg ataagcgcga
    42781 ggattttttg tcatctggta atttttataa ataccatata gaccgacacc ttgtggccaa
    42841 ttccacactg tccagctttt gtcatctact ttcagtccat caaagtccaa caaaaattca
    42901 ccattatcgt ctttgatcgt tgtcaggttt tcaacgagca ggtcaatttt ttctttcact
    42961 aattctttag gtacaaaaat ctcttcttta ataatcatct ttacgccacc tttttaaacg
    43021 ctttcatttt atatccttaa tatagcagta ataccgtcca ctataaatat cgctttttga
    43081 acaaaaactg tctaaaattg acctaaatgt taaataacaa aagtaacttt cagttacttg
    43141 gaaattaaaa aaagaacttg gagaaacaac gttttccaag ttctttttca tctattttct
    43201 tatctttttt ctacgctttc ttttgcttat ccgttgtaag catcaatgat gatctgtttc
    43261 aattcagaga tcaatggttc tttcggatta gctgtcgtac attggtcttc gtaagctaat
    43321 tcagccatac ggtctacggt tgtatctagt gtttcttgtg tgactccttg agcttttaga
    43381 ctcatgttga tgccgacaga ttttcctaat tcataaacag ccgttgctaa agcttcgact
    43441 aattcctctg ttgtttcgcc tttcagacct aagaattttg cgatatctgc gtaatctgta
    43501 tctgcacgga aatagtcata tttagggaac atcgcatgtt tttgtggatc ttttgcatta
    43561 tagcggataa tatgcggtaa aagaatcgca tttgtccgtc catgagggat cccgtattcc
    43621 ccgccgattt tatgtgcgac tgagtgacaa atacctagga atgcgttagc aaacgccata
    43681 ccagccattg tagaagcgtt gtgcattttt tcacgtgatt ccatatcagg tgtttttact
    43741 gatttttcta aatagtcaaa gactaacttg attgcttgta ggctcaatcc acgtgtataa
    43801 tctgaagcca tgactgaaac ataggattcg attgcatgtg ttaacacatc catcccggta
    43861 tctgctgtga cagatgctgg aactgacatg acgaattgag gatcgacgat tgcgacatct
    43921 ggcgtcaatg cataatctgc taacggatat ttcacatgtg tttcactgtc agtgatgacc
    43981 gcaaatggcg ttacttcaga accagtccct gatgttgtcg ggatacatac gaattgtgtt
    44041 ttttctggtt tgtcgatctt atatgtccgt ttacggatat ccaagaattt ttgttttgca
    44101 ccaaagaaag atgtgtctgg gtgttcatag aacatccaca ttcccttagc tgcgtccatt
    44161 gcagaaccgc ctccaagagc gattactgta tctggcttga agtctgccat cagtttcgtt
    44221 cctgcgtata cggtatttgt cgatgggttc ggttcaacat ctgagaagac ttcgatcttc
    44281 acatcatttt tacgtcgtgc caatacttca cgaactgtat cgcagtaacc gaattggacc
    44341 ataccagggt cacaaacgat catgacacgt tcaacgtttt ccattttctc taagtacaat
    44401 agagagttct tttcaaagaa gatttttggc ggtaatttga accattgcat gttatttctc
    44461 cgtttcgcta cagttttgac attgattaag tttacagcag agacgttacg agaaacagag
    44521 ttcttaccat aagaaccgca tcccaaagtc aatgacggaa tcatttcgtt atagatatca
    44581 ccgatgccgc cttctgcaga tggtgtattc actaaaatac gacaagcttt cattcgaaga
    44641 ccgaatttga catgcaactc ttcgttttct gaatggataa ctgctgtatg tcctaatcct
    44701 ccaagattca acataccttc acacaaatca aacgcatggt ccgtgttgtt agctttgatc
    44761 atggcaagaa ctggagaaag tttttctctt gatagcggat aatctgggcc tacgccttcc
    44821 aattcagcaa tcagcatttt cgttcctttt ggtacactga ttcctgccaa ttttgcgatt
    44881 tccatcgcag aatgtccaac gattgatgga ttgaccgcat attttccttc gttcatcact
    44941 gcatcttcta atttttgcaa ttcatctggt tttacgatat aaacttggtg tgcttggaat
    45001 tctgctttta ctgctgcata gatttcttta tccacgatca cagcttgttc agaagcacaa
    45061 atcattccat tatcaaatgt tttcgaaacg atcaaatcgt tcactgcacg tttaattttt
    45121 gctgtttttt caatataagc tggtacgtta cctggaccta cacctaaagc tggttttcct
    45181 gtagagtaag cagacttgac cattccagct ccacctgtcg ctaagacgat cgcgatacct
    45241 ggatggttca ttagcataga agtcgcttcg atcgaaggat gttcgatcca ttggatacaa
    45301 ttttcaggag ctccggcagc gatagcagca tcacgtacca ctcgtgctgc ttctgcagaa
    45361 gatttttggg cacttggatg gaaagcaaaa acgattggat ttcttgtttt caacgcgatc
    45421 attgatttga aaatcgttgt ggaagtcgga ttcgttgttg gagttactcc gcaaaccaca
    45481 ccgactggtt cagcaatttc aatcaaccct ttttggacat cttcattgat cacaccaact
    45541 gttttatcat gtttgatatt atgccaaata tattcggacg catacatatt tttgattgct
    45601 ttatcttcgt aaacaccgcg gcctgtttcc tctacagcca tcttcgctaa tggcatatgt
    45661 tgatccaaag ctgccattgc catttcatgc acgatatggt ctactttttc ttgatcaaag
    45721 ctttccattt ctttcaatgc tgcatttgct ttttctgcca aatcatcaat cattgcttgt
    45781 acgtcaattg tcttcgtttt ttctttttct attactttag ccatcattat cctcctaatg
    45841 ggtcatggtt atttgttaaa taattcacat gtatatcata caatatttat ttttctatgt
    45901 aaacactttt ttgtgatttt tttcacagtt atttttaaat ataaaattgc tttatttaca
    45961 ggtgtttgta tagaaaaata tataaagtta tttattatta tttatatgac acacccgatt
    46021 attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga
    46081 ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc
    46141 cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc
    46201 tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt
    46261 gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac
    46321 tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac
    46381 tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg
    46441 cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa
    46501 tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa
    46561 tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc
    46621 gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc
    46681 atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta
    46741 agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg
    46801 tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc
    46861 atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc
    46921 gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa
    46981 tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt
    47041 agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat
    47101 tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc
    47161 aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt
    47221 caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact
    47281 ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg
    47341 gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa
    47401 atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc
    47461 tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc
    47521 tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc
    47581 acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg
    47641 catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc
    47701 agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc
    47761 tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt
    47821 gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc ctttttttat
    47881 gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat ggtatattgg
    47941 tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc
    48001 aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt
    48061 gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat
    48121 atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg
    48181 cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac
    48241 ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg
    48301 tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt
    48361 tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc
    48421 tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact
    48481 ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt
    48541 agatctttca tttatcggct ggcatatctt agcattagca actgctggta ttggttatct
    48601 atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga
    48661 tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag
    48721 aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca
    48781 cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc
    48841 ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac
    48901 gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag
    48961 ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat
    49021 ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac
    49081 attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac
    49141 atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc
    49201 aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca ataaacctcg
    49261 agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg
    49321 ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg
    49381 cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc
    49441 cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg
    49501 tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc
    49561 ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt
    49621 caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg
    49681 ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc
    49741 tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc
    49801 ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg
    49861 atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt
    49921 actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc
    49981 cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat
    50041 cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc
    50101 tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc
    50161 gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac
    50221 tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac
    50281 gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt
    50341 cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc
    50401 ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg
    50461 tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc
    50521 tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt
    50581 caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt
    50641 catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc
    50701 agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg
    50761 tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat
    50821 gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac
    50881 catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc
    50941 aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat
    51001 caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac
    51061 cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt
    51121 attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa
    51181 taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc
    51241 tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa
    51301 atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt
    51361 attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg
    51421 atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt
    51481 cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta
    51541 tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct
    51601 tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat
    51661 caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac
    51721 aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg
    51781 tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta
    51841 aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac
    51901 aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt
    51961 acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag
    52021 ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg
    52081 cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc
    52141 ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa
    52201 catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa
    52261 ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt
    52321 aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt
    52381 cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa
    52441 ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa
    52501 ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa
    52561 ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata
    52621 gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat
    52681 ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg
    52741 tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat
    52801 tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg
    52861 tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa
    52921 acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc
    52981 aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa
    53041 acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa
    53101 atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc
    53161 tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc
    53221 ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga
    53281 tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca
    53341 aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt
    53401 gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc
    53461 aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa
    53521 attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg
    53581 cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa
    53641 gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg
    53701 ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt
    53761 gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca
    53821 atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg
    53881 atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc
    53941 atgagtgaag gccgtgtgat caatttgata caccagaaaa tattttacgt catcctgcaa
    54001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    54061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    54121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    54181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    54241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    54301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    54361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    54421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    54481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    54541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    54601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    54661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    54721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    54781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    54841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    54901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    54961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    55021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    55081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    55141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    55201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    55261 agcacagatc ttagcaagtc tagttgccgg aatggtttaa cattatacag ataaaaatac
    55321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    55381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    55441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    55501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    55561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    55621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    55681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    55741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    55801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    55861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    55921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    55981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    56041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    56101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    56161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    56221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    56281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    56341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    56401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    56461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    56521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    56581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    56641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    56701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    56761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    56821 cttatgggca aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    56881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    56941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    57001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    57061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    57121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    57181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    57241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    57301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    57361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    57421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    57481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    57541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    57601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    57661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    57721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    57781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    57841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    57901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    57961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    58021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    58081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    58141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    58201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    58261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    58321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    58381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    58441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    58501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    58561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    58621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    58681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    58741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    58801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    58861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    58921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    58981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    59041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    59101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    59161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    59221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    59281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    59341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    59401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    59461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    59521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    59581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    59641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    59701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    59761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    59821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    59881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    59941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    60001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    60061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    60121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    60181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    60241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    60301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    60361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    60421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    60481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    60541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    60601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    60661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    60721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    60781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    60841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    60901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    60961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    61021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    61081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    61141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    61201 aataaacttt aagaaaataa ttggaaaaaa acagaaaa
//
LOCUS       DASJTM010000005        54508 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJTM010000005 DASJTM010000000
VERSION     DASJTM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.005"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..610
                     /locus_tag="U4B29_000282"
     CDS             <1..610
                     /locus_tag="U4B29_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105254.1"
                     /translation="NFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDIT
                     EAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSF
                     NHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLAL
                     MPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(744..869)
                     /locus_tag="U4B29_000283"
     CDS             complement(744..869)
                     /locus_tag="U4B29_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105255.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1003..1896)
                     /locus_tag="U4B29_000284"
     CDS             complement(1003..1896)
                     /locus_tag="U4B29_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN2105256.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2078..3004)
                     /locus_tag="U4B29_000285"
     CDS             complement(2078..3004)
                     /locus_tag="U4B29_000285"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN2105257.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3089..3850)
                     /gene="pflA"
                     /locus_tag="U4B29_000286"
     CDS             complement(3089..3850)
                     /gene="pflA"
                     /locus_tag="U4B29_000286"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN2105258.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4094..6325)
                     /gene="pflB"
                     /locus_tag="U4B29_000287"
     CDS             complement(4094..6325)
                     /gene="pflB"
                     /locus_tag="U4B29_000287"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN2105259.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6602..9052)
                     /gene="parC"
                     /locus_tag="U4B29_000288"
     CDS             complement(6602..9052)
                     /gene="parC"
                     /locus_tag="U4B29_000288"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN2105260.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9064..11109)
                     /gene="parE"
                     /locus_tag="U4B29_000289"
     CDS             complement(9064..11109)
                     /gene="parE"
                     /locus_tag="U4B29_000289"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN2105261.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11275..11709)
                     /locus_tag="U4B29_000290"
     CDS             complement(11275..11709)
                     /locus_tag="U4B29_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN2105262.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11836..12474
                     /gene="plsY"
                     /locus_tag="U4B29_000291"
     CDS             11836..12474
                     /gene="plsY"
                     /locus_tag="U4B29_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN2105263.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12606..13481)
                     /locus_tag="U4B29_000292"
     CDS             complement(12606..13481)
                     /locus_tag="U4B29_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN2105264.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13563..14354)
                     /gene="codY"
                     /locus_tag="U4B29_000293"
     CDS             complement(13563..14354)
                     /gene="codY"
                     /locus_tag="U4B29_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN2105265.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14372..15772)
                     /gene="hslU"
                     /locus_tag="U4B29_000294"
     CDS             complement(14372..15772)
                     /gene="hslU"
                     /locus_tag="U4B29_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN2105266.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15786..16334)
                     /gene="hslV"
                     /locus_tag="U4B29_000295"
     CDS             complement(15786..16334)
                     /gene="hslV"
                     /locus_tag="U4B29_000295"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN2105267.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16371..17276)
                     /gene="xerC"
                     /locus_tag="U4B29_000296"
     CDS             complement(16371..17276)
                     /gene="xerC"
                     /locus_tag="U4B29_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN2105268.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17474..18850)
                     /gene="trmFO"
                     /locus_tag="U4B29_000297"
     CDS             complement(17474..18850)
                     /gene="trmFO"
                     /locus_tag="U4B29_000297"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN2105269.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18819..20897)
                     /gene="topA"
                     /locus_tag="U4B29_000298"
     CDS             complement(18819..20897)
                     /gene="topA"
                     /locus_tag="U4B29_000298"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN2105270.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21019..21876)
                     /gene="dprA"
                     /locus_tag="U4B29_000299"
     CDS             complement(21019..21876)
                     /gene="dprA"
                     /locus_tag="U4B29_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN2105271.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21932..22699)
                     /locus_tag="U4B29_000300"
     CDS             complement(21932..22699)
                     /locus_tag="U4B29_000300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN2105272.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22692..23552)
                     /gene="ylqF"
                     /locus_tag="U4B29_000301"
     CDS             complement(22692..23552)
                     /gene="ylqF"
                     /locus_tag="U4B29_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN2105273.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23812..24210
                     /locus_tag="U4B29_000302"
     CDS             23812..24210
                     /locus_tag="U4B29_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2105274.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24489..24989
                     /locus_tag="U4B29_000303"
     CDS             24489..24989
                     /locus_tag="U4B29_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2105275.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25072..25623)
                     /gene="lepB"
                     /locus_tag="U4B29_000304"
     CDS             complement(25072..25623)
                     /gene="lepB"
                     /locus_tag="U4B29_000304"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2105276.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25775..26002)
                     /locus_tag="U4B29_000305"
     CDS             complement(25775..26002)
                     /locus_tag="U4B29_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN2105277.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25999..26517)
                     /gene="msrA"
                     /locus_tag="U4B29_000306"
     CDS             complement(25999..26517)
                     /gene="msrA"
                     /locus_tag="U4B29_000306"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2105278.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26537..27250)
                     /locus_tag="U4B29_000307"
     CDS             complement(26537..27250)
                     /locus_tag="U4B29_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN2105279.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27253..28149)
                     /locus_tag="U4B29_000308"
     CDS             complement(27253..28149)
                     /locus_tag="U4B29_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2105280.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28307..29149)
                     /locus_tag="U4B29_000309"
     CDS             complement(28307..29149)
                     /locus_tag="U4B29_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2105281.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29281..29934
                     /locus_tag="U4B29_000310"
     CDS             29281..29934
                     /locus_tag="U4B29_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN2105282.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29997..30524)
                     /locus_tag="U4B29_000311"
     CDS             complement(29997..30524)
                     /locus_tag="U4B29_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2105283.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30544..31491)
                     /locus_tag="U4B29_000312"
     CDS             complement(30544..31491)
                     /locus_tag="U4B29_000312"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN2105284.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31503..33389)
                     /locus_tag="U4B29_000313"
     CDS             complement(31503..33389)
                     /locus_tag="U4B29_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2105285.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33549..35627)
                     /locus_tag="U4B29_000314"
     CDS             complement(33549..35627)
                     /locus_tag="U4B29_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN2105286.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35640..35915)
                     /locus_tag="U4B29_000315"
     CDS             complement(35640..35915)
                     /locus_tag="U4B29_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105287.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36277..36732)
                     /locus_tag="U4B29_000316"
     CDS             complement(36277..36732)
                     /locus_tag="U4B29_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105288.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36886..38094)
                     /locus_tag="U4B29_000317"
     CDS             complement(36886..38094)
                     /locus_tag="U4B29_000317"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN2105289.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38206..38538)
                     /locus_tag="U4B29_000318"
     CDS             complement(38206..38538)
                     /locus_tag="U4B29_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2105290.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38686..39561
                     /locus_tag="U4B29_000319"
     CDS             38686..39561
                     /locus_tag="U4B29_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2105291.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39643..40224)
                     /locus_tag="U4B29_000320"
     CDS             complement(39643..40224)
                     /locus_tag="U4B29_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN2105292.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40230..41489)
                     /locus_tag="U4B29_000321"
     CDS             complement(40230..41489)
                     /locus_tag="U4B29_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2105293.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41491..42537)
                     /locus_tag="U4B29_000322"
     CDS             complement(41491..42537)
                     /locus_tag="U4B29_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105294.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42772..43047)
                     /locus_tag="U4B29_000323"
     CDS             complement(42772..43047)
                     /locus_tag="U4B29_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN2105295.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43290..44600)
                     /gene="der"
                     /locus_tag="U4B29_000324"
     CDS             complement(43290..44600)
                     /gene="der"
                     /locus_tag="U4B29_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN2105296.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44782..46011)
                     /gene="rpsA"
                     /locus_tag="U4B29_000325"
     CDS             complement(44782..46011)
                     /gene="rpsA"
                     /locus_tag="U4B29_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN2105297.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46146..46826)
                     /locus_tag="U4B29_000326"
     CDS             complement(46146..46826)
                     /locus_tag="U4B29_000326"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN2105298.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46875..47510)
                     /locus_tag="U4B29_000327"
     CDS             complement(46875..47510)
                     /locus_tag="U4B29_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2105299.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47576..48979)
                     /locus_tag="U4B29_000328"
     CDS             complement(47576..48979)
                     /locus_tag="U4B29_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN2105300.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48966..49997)
                     /locus_tag="U4B29_000329"
     CDS             complement(48966..49997)
                     /locus_tag="U4B29_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105301.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50028..50249
                     /locus_tag="U4B29_000330"
     CDS             50028..50249
                     /locus_tag="U4B29_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN2105302.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50414..51151)
                     /locus_tag="U4B29_000331"
     CDS             complement(50414..51151)
                     /locus_tag="U4B29_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN2105303.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51304..52644)
                     /locus_tag="U4B29_000332"
     CDS             complement(51304..52644)
                     /locus_tag="U4B29_000332"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN2105304.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52881..54404)
                     /locus_tag="U4B29_000333"
     CDS             complement(52881..54404)
                     /locus_tag="U4B29_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN2105305.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        14848 a        11520 c         9301 g        18839 t
ORIGIN      
        1 taatttttta catttaggct ctttttttgc aactttgacg atcttatttt ttctatgtta
       61 ttcatacctt aagacctcac atgcgccttc tagtgcatta ggctggttgc ttttggatca
      121 ttctgatatc acagaagccg aagaaaatcc gttttttttg attttgtctt ctctttgcgg
      181 cttactgttt tctgtttatt tcggtgccta tttttttcat cgacatcctg gtacgttaaa
      241 cgaagcttca agcgtattga ttgcatttaa aacgttttct tttttagcaa ttctttttta
      301 ttcgtttaat cacaatacgc ttttcttctt tatcatgaat ggtttagtcg tgattttatc
      361 catttttact tattttattt tttcactcat ttttagccaa ttgaaatgtc cgctcttctt
      421 tcgattagtt cctgtcacct gttttattta cagcttgttt tccttttttg tccttgcttt
      481 aatgccactg accaaccctt cgacgatcca agtcgcagga aatctgttgg atatgctctt
      541 catcgtcagt ctaggtgtat ttatgtggca aagaaaaaac aaacaaaata aaaagcattt
      601 tgaagcctga ctattacgac ttcataaatg cttttttagt tttaaacact tcaatctaaa
      661 agaataaccc aagattggat aaattattaa tgactcgcca ttcgtttctt agtgattctg
      721 gattatcaaa tggacttcct aatctaatca gctagttctt cccataaaat gccatacttg
      781 actgaatcat aatattgatt atttaaaaat ctaacttttc ttatcactac ttcctttgtc
      841 attccacatt tctcaccatt tttttgcatt cctttgtttc cagaccatgt agtgaatcta
      901 ataggtggta gataggcaaa atactcaaat aattctttta accataaagg aagagaaagg
      961 ctcctcctgc aagagtcagt ataatttttg cttactgctc tcttaggttt tactcactaa
     1021 aactgttcca tattctttga tataagaaaa tgcatgcggt ataaatttct tgctcagctc
     1081 agaaaagatt tgtcgtttaa gaaaggctat ttcctccatt gtcaatgaag gatccaatgt
     1141 tggtaattca atctcaaaat tgtttttttc gacaataggt aattctaatg tgaatgattc
     1201 ttcttccttt atagtattcg tcacaagtag agactgatgg aaaatactag gccggcgatt
     1261 tttatattta ttcttacgta tcctgtcaac gataacttct atttgatcaa aaaagtacga
     1321 ttcatttacg tctccaataa gttcggctaa ttctttgaat gccttcgtta caggaagctg
     1381 attcttctgt acaaaaaagt aattagccaa agtaagcggg aagtgttcta tagaggaaag
     1441 tgtgataaaa cggtacttct catttcctgc agttgtcctc agcaattctg tctctcggct
     1501 gtttcttact ccataaaaat acgcttcttt ccgttcatct tcgaaacaat acgcccaaat
     1561 aacttctccc atcgctaatt tttgttcatc aacagagagt ctcttcaatt gttcagctat
     1621 ggtttcagca gccgaagtgg cttgttgcaa gtaatcttta ggatcaaata ttggaaagtt
     1681 caaatgatat cttcgttctt ccctcttgat taatcctgca tcgatcaata aatcaagaaa
     1741 atgggaaaat ttattttgcg aaaattcttt tttcaattct cttagaatcg tttcttgttc
     1801 tcgatgttca tatagataat cggtcaatga agaaaaaagc ggatcatcgg tcagtttttg
     1861 ataaaagttt tccttatttt ttgaataata gttcatatat gttctctttt ctatccgttt
     1921 tattttactt aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat aattgactgt
     1981 tattctcctc catttcttcg gcctttagta tgttctatgt ttctttttat atcaatcgct
     2041 tatccattca ttttgaatgc ttcatactac catttgatta gctaaataca gccgtcaatt
     2101 gaggtacgac ttgtttttta cgtgaaacga cacctttaag caaagcacgg ttgttggtca
     2161 aagttgtttg gaaagcttct tctacttttt cttttccttt accagcaaca agtaattctg
     2221 aatcactgtc taagatgttt gtgacaacta atacgaacag atcgtagcca tttgctttca
     2281 tttcatcttc cattgctgct tctagctctg cttgtcgatc catcacttca ttaaggtcca
     2341 ctgtattgac ttgagcgata cgaacgttgg aactgcccat tgggaaactt tttgcatcca
     2401 aatcaagaag aaccgcagct gatttatcac ttagatttgt tcctgctttc aacatttcaa
     2461 gtccataagt gtttagatcg acttcagcga ttgctgcaag ttcttttgca gctgcttcat
     2521 cttcaggcgt acatgttggt gatttgaaca acaatgtatc tgaaatgata gctgagagca
     2581 tgattcctgc gatttcttta ggaattgtta cttggttttc tttatatagt tttaaaataa
     2641 tcgtacttgt acatccaact ggttctgcac gatagtacaa tggattagct gtttcaaagt
     2701 ttgcaatacg atggtggtct actacagcta aaatatttac ttcgctgata tctgaaatac
     2761 tttgttggaa ttcattatga tccacaagca tgacatctgc attttctgct ttagctgact
     2821 ctactactcg tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt tcttcattag
     2881 gtgtacctag agcaactgct tctgtttctt tacctaattc attttgcaag tgtgaaaacg
     2941 caatggctgc cccgattgca tctgtatctg gattttggtg accaaaaact aaaatttttg
     3001 acatgtcatt cgctcctttg tgatttctcc tatttaataa tagcaaaaaa acggaaagaa
     3061 gcaatactta tcttctttcc gatgcgacct atcgcgttaa atattttgta tactcttctg
     3121 tatgaagcaa tcgtttagca ttttcgacac gttcttttgt aggtggttcg atcccttcta
     3181 atggatattt caaaccaagt gtttcccact tgtattttcc catcgtatga taaggaagaa
     3241 tctctactct gtctacattt gacaaggttt gaataaagtt gtctagtcgg atcaaatatt
     3301 catcataatc acttcgctca ggaactagta catggcgaat ccaaaccggt ttgttgattt
     3361 ttgacaaata ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt agttttttat
     3421 gctcttcatt atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt
     3481 cttgaaaacg tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat gtatccaatg
     3541 tagtatgcat cttccgctct tttgcttttt taaatagttc gatcaaaaaa tcgatctgca
     3601 atagaggttc tccaccacta acagtaatac cacctttatc tccccagtaa ggtctgaagc
     3661 gttctgcttt atctaaaagt tcatctgctg tgtattcttt ccctccgcca atattccaag
     3721 tatctggatt gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac acgacaaagc
     3781 gcagtccagg tccgtctact gaaccgaaag tttcgataga atgaacatag ccgattgttt
     3841 tttcttccat ttaagttcac atcttttctt tgatttcatt ttaagtacaa gttagctgtc
     3901 gaagcgaagc tttctgaaca aaatttttcg caattgccgc ttcgttatgt gtaaaattta
     3961 cagggacgga acatacaagt cctgcccctg taaatcgttt ctttcaaata agcatactga
     4021 ttattcgatt ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt
     4081 tttcacgaac tttttacatt ctgtcgtgtg aagttctcat caagacatct aattgttgtt
     4141 ctcttgtcaa gtcacggaat ttaactgcat aaccagatac ccggatcgtc aagttcggat
     4201 atttcattgg attttccatt gcatcacgca ataaatcatt actgaataca ttgacattca
     4261 aatgataagc tccttttgag aagtatccat ctaagacatt acgcaagttt tcgattcttg
     4321 tatcttgttc ttttccaaga cctgaaggat tgattgtttg ggtatttgaa attccatcca
     4381 atgcacagcc gtaattcatt ttagctgttg agttcaggct agctaataac ccactttgtt
     4441 ccgcattgta acttggattt gctcctggcg caagtggttt tccagcttca cggccatcag
     4501 gagtatttcc tgtattttta ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg
     4561 ttggttttga gttgcggtaa gttttatgac gtttgatttg tcccatgaag tattccaaga
     4621 tccatgaaac taattcatct gcacggtcat catcatttcc gtatttaggg aagtcgccat
     4681 ccacttcaaa atcaactgct attccttttt cattacggat tgcttttact tttgcatatt
     4741 tgatcgctga caagctgtct gccgcatgag agatcccagc aatacctgta gcaaatgtgc
     4801 gttgcaagtc agaatccatc aaagccagct gagctgcttc ataagaatat ttatcatgca
     4861 tataatgaat gatgttcaac gtattgacat atagttctgc taaccaatcc atcatattca
     4921 tgaagttatg tttcacttct tcaaagtcta gtatttctga tgtgattgga cgatattctg
     4981 gtccaacttg ggcgccagtc acttcatcga ctccgccatt gatggcatat aatagtgttt
     5041 tagccaagtt agcacgagcg ccaaagaatt gcatgtcttt tccgacaact gttcctgata
     5101 cacagcaagc aatcgcacaa tcatcagaac cccattgttc acgaagctga ttatcatttt
     5161 cgaattgaat cgaagaactt tcgatagcga ttctgctagc atatgttctg aatccttctg
     5221 gaaggtgttc agaatacaaa acagtcaagt ttggttctgg tgaaggtccc atatttgtta
     5281 atgtatgaag cagacggaaa tcattctttg taactaatga acgaccatct aatcccatac
     5341 ctgcaatgga taaagtagcc cagataggga atcctgagaa taattggtta tattcaggtg
     5401 tacgcgcgaa tttgaccata cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt
     5461 ctttttctgt gattactcca ttatccaaat cacgttggat atagatatca aggaatgctg
     5521 agatacgtcc gattgacatc gcagcaccat tttgtgattt gattgctgct aaatatccga
     5581 agtatagcca ttggacagct tcttttgcat ttgcagctgg tttagaaata tcatagccat
     5641 atttcgcagc catttctttc atttgcgcta atgcacgata ttgttcagtg atttcttcac
     5701 gtaaacgaat cacttcttca ctcattgtat gataaccagt catatcatga tcttttttct
     5761 tttgttccat caaatagtcg attccataca atgccacacg acgatagtca ccgatgatac
     5821 gtccgcgacc gtaagcgtct ggaagccctg taatgatttt gtttttacga gcagcacgca
     5881 tttcagcagt ataagcgtca aatacacctt gattatgtgt tttgcgccag tcagtgaaaa
     5941 tatgtgtcaa ttcatcgttt ggtgtatagc cattgctttt tagcgcattg ttagccatat
     6001 tgattccgcc gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga
     6061 ttttttccaa atctttatcc aaatagcctg catcgtgtga agtaatagtt gatacgattt
     6121 ctgtgtccat atcatagaca ccgttacgct cgaattggat atcatttaac ttttgcaatt
     6181 catcccataa tttttctgtt gcttctgttg ggccttccaa aaagctgtca tcaccgcgat
     6241 attcagtgta gttattttga ataaagtctc tagtgtctac gccttctttc catttggtac
     6301 ctttaaatcc attccattgc tccattgtat ggacctccta aatttctgtt ttatgtaaca
     6361 gtttttacgt gattagtata acacagcacc ctatctttgc aagggttttc ttgtttttta
     6421 tttaacaatt tttatgtttt atttacatat atttaaatca acgttgacat accaacattt
     6481 aagcattgtg aacactttca tctttttttg caaacgtata aggatttatt aaaaatgaat
     6541 gaaaaagaga cgccacctgt attaatacag atggcgtctc ttcattaaaa tactacaaca
     6601 gttacgactc gatgaaaatc tcaggagcat cgataacttc gatgacttct ccaccttttt
     6661 tctcatccat cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa tcagttgggt
     6721 caatcgttaa ctgctgccct ttttgattca tcaaggttat ttcttttgat cttccttccg
     6781 acatatagat aatacggtgc ggattttttt tcagctcacg catgaccgtc actccccgct
     6841 tcgcacgtga agtttggctg atttcttgag caagcatgcg tttaacagct cctcgctgag
     6901 taacgattac gatcggcgta tcaccttctg ctaacacaag cagcccgttg ataatcctat
     6961 ctccatcttt caaattcata gacttcactc cagctgcctt tgctccaaca acgggaacat
     7021 ctgttaaagg atagcgcaaa cccattcctt gatgcgttac taagaaaaca tctaagcctg
     7081 gctgatcttc tttcagatac acagcaatca gttcatcttc ttccgccttt agcttcatac
     7141 agctgagcgg acggctctta taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct
     7201 tgatcaatcc attcttgctg atgaatacaa atgttttctc tgcttgaatt ttttgatatg
     7261 ggaaaacagc taaaatcgat tcgtctacag acagattgga gattgtttga gaaatgtgct
     7321 caccagcatc tttccatttc aaatcgggta gttcatggac agggcggtag atcacatttg
     7381 ctttatttgt gatcagaaga acatggtcca atgtactcaa ttcacctgag tatagcaagt
     7441 agtcgccttc tcgcatcccg atttcttctg gtttggaagc actataagaa cgaaggcttg
     7501 ttcgcttcac atagccttct cgcgtcacac tgacaattac gtcttcttgt gccactaaga
     7561 cttgcgtatc aattttgatt tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag
     7621 aataattttt tttcacttct cgcaattctt ttttcataac agagaaaagc tctttgtcat
     7681 ttgaaaggat tttattcaac tctgtgattt gcgcaatcaa gctttcggat tccttttgaa
     7741 gctctgtgat atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt
     7801 gttcttctgt aaattgaaag acgtcgacta aattcttttt ggcgtctttt ttgtctttac
     7861 tttcacgaat cgttgcgatt acttcatcca gaatcgataa agctttcatc aatccttcta
     7921 cgatatgctg tcttttttga gctttggcta aatcgaattg actacgtttt gttatgactt
     7981 gctttcgatg ctcgatataa cttcttaaaa tgtctttcag tccaacttga tgcggagtca
     8041 tatggtcgat agcgaccata ttgaaattgt aattgatctg tagctcagtg tttttgaaca
     8101 aataattcaa aattccttga gcattggcgt cttttttcaa ttcgacgaca atttgcagac
     8161 ctgttctatc actttcatct cgtacttcag ctattccatc gattttctta ttcaaacgga
     8221 tctcatccat ttttttcacc aaggtcgctt tatttacttc atatgggatt tctgtgatca
     8281 cgatttgctg tttattaccc ttcattgcct ctatctttgt tttcgaacga agaataactt
     8341 ttcctcgtcc tgtttcataa gcttttttga tttcatcttt tccttgcaag atccctcctg
     8401 ttggaaagtc aggtccagga atatattcca tcaatttttc aaggctcgct tgtggatgat
     8461 cgatcatata gattgtgcca tcgatcactt cagctaagtt atgagtcggg atttctgttg
     8521 catatccagc agatatacct gtcgatccat tgacgagaag gtttgggtat cgagctggaa
     8581 ggactgttgg ttctttttct gtatcatcaa aattccacac gagatcgact gtttctttct
     8641 cgatatctgc tagcatctct ccacttaact tggataatct ggcttcagta taacgcatcg
     8701 cagcaggtgg atcaccgtcc atactcccgt tattcccgtg catttcgatc agtacttccc
     8761 gcaacttcca gtcttggctc atgcggacca tcgcttcata aatactgatg tcaccatgag
     8821 gatgataatt ccccatgata tttccgacag attttgctga tttacggaat cctttgtcat
     8881 acgtattgcc atctttgttc atggcaaata atatccggcg ttgcactggc ttcaatccat
     8941 cacggatatc aggcagtgca cgttcttgga taatatattt ggaataacgt ccgaagcggt
     9001 cacccatcac ttcttctaaa gttaattctt gtacttcttg tcgattttcc atcaggtgac
     9061 ctcctattct acttcaaata actgattgtt ttcattttta tctgctcgtt cttcatctaa
     9121 taaatcatta gaaactgaag gagagatttc agtatcctct tttttatcta agatactgcc
     9181 atcttcttcg agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag gttctacttt
     9241 gtcacccatc agtgtggtta ctcgacgttc agcttgcgca gcatcatcga tacggacgcg
     9301 gatcaacgta cgggaagtcg gatccatcgt tgtttcccac aattgttccg cattcatctc
     9361 tccaagtcct ttatatcgct gcagcatata tcctttgccg acttttttaa tcattgctgc
     9421 caattcgtca tctgtccacg catattcaat gacttgcttc tttccttgtc ctttggatac
     9481 tttatataaa ggcggtaacg cgatatatac tttcccagct tcaatcagcg gtttcatata
     9541 acgataaaag aaagtaagca gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt
     9601 catgatgatt actttgtcgt aattacagtc ttcgatagaa aactcaggac cgactcctgc
     9661 accaatcgta tagatcatcg tattgatttc ttcatttttc aagatatctt gcattttagc
     9721 tttttcggta ttgatgactt ttcctcgtaa aggtagaatt gcttggaact ttctgtctct
     9781 tccttgttta gcagatccgc cagcagaatc cccttcgact aaatacaatt catttttctt
     9841 agggtttcgg gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc cttttttacg
     9901 cttcttccca tttcggcttt cttctctggc tttacgtgct gcttctctcg cttctctggc
     9961 tttgattgct ttacggacca acatctggct catttcgcta ttttcctgca aataaaagcc
    10021 catttgttca ctgatgacgt tatctactac ggtacgagca actggcgtac caagtttttc
    10081 ttttgtttgc ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa gtacagcagc
    10141 taacccttct ctgaaatcac taccttccag attcttgtcc cgttctttca gcaaaccaac
    10201 ttttctcgca tattcattat aagatttcgt catcgccgct ttcataccag cttcatgggt
    10261 tcccccatct tttgtccgaa cgttattgac aaaagataaa acattttctg aataaccgtc
    10321 attgtattga taagcgacct ctacttcgat accttctttt tctcctgaaa aataaacaac
    10381 tggtgtcaat gtatcttttt cttcattcag ataatcaaca aattctttga ttccttcttc
    10441 gtaatgaaaa atttccttga ctggttcttc tccacgaaga tcagaaagag agattttaac
    10501 gccttttaga agaaaggcag attctcttaa ccgttcggct aaaatttcat atgaaaattt
    10561 cgttgtactg aatattgtat catctggtaa aaaatggaca gtcgtcccgt ttgctttttt
    10621 tgtttttcct atctttttca aagtaccgtc aggtttaccg ccgtttttaa atttctgctg
    10681 atactctacg ccatctctaa cgatcgttac aattagccat ttcgataagg catttaccac
    10741 actggctcct acaccgtgca gtccaccaga agttttgtac cctccttgac caaactttcc
    10801 gccagcatgc agcacagtaa aaatcacttc aactgttggt attccggaag catgcattcc
    10861 tacaggcatc ccacgtccgc tatctgcaac agttatacta ttgtcttcat ggatggtcac
    10921 atcaatctca ctgccataac ctgataatgc ttcgtcaact gcattatcga cgatttcata
    10981 gactaaatga tgcaagcctc ggctgtcagt ggatccaatg tacatccctg gtctttttct
    11041 aactgcctct aatccttcca atacttggat ggacgagtca ttgtattcgt tgtttacttt
    11101 ttttgccaag gcaaacgctc cttcaataat actttgcggc aaaaccgcac cacgattatc
    11161 atactagaaa aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt aattatctcc
    11221 taaccgcttt ttcgacattc ccctctatcg atcatctctt ctttgtggtc atttttattt
    11281 ttggttttct ttcaacttac caagttcaat cttaatacaa cgatccatta cgatatctgt
    11341 acgtcctgct tttcttagca tctcagctgc ttctttgttt tcaatcccta attgtgccca
    11401 gaacacttta gcatcagttt tcaaaaaatc acctgctatt tcaggtaaaa attcactccg
    11461 tctaaataca tctacgatat cgattggatc atcgatttct gtcaaattcg catagacttt
    11521 ctcgcctaga atttcttcac cagccaataa tggatttact ggtatgattt tatatccatt
    11581 gtcttgcaat acctgtgcaa ccatgtaact tgttcgttct ggtttattgc tcaagcctac
    11641 gacggcaata ttttttgctt cttccaaata gttgaaaatc gtttcttgtg ttggattttt
    11701 tacagacatt tctgccaccc cttgcttttc tttttattcc atctattata cttgtatctt
    11761 tcattttttc aattgttcct gttacctatt catgctaaaa taaaggatag ttttcaactt
    11821 gaaagagggt aaaaaatgaa attgatctta ttacttattt tagcatatct tttaggttct
    11881 attccttccg gtgtctggat tggcaaattg tttttcaaaa aagatatccg acaacacggg
    11941 agcggtaata ctggaacgac caatacattt cgtgtattag gcaaaacagc cgggattgtt
    12001 gtcctgctta tggatatttt aaaaggtaca ttggctactt gtttgccgat gatcttccac
    12061 ctttctggga ttaaccctct ttggtttggg gtatgcgcca ttcttggcca tacatttccg
    12121 atttttgctg ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat gcttttagga
    12181 tttaatcctg tattttttgt ttattcttgt atcatcttta tcgttagttt attttgtaca
    12241 agtatggtga gtttgagcag tatgatcagt gcagtattga tcactttatc tacgatcgtc
    12301 cttccttatg tagctcctgt gattttggct aagcccaatt ggttactgac gatcattgcc
    12361 tttttagttt cttcctttat cttttatcgg cataaagaca atatcaagcg tatcagaaac
    12421 catactgaaa gtcgaatccc gtttggtttg aatagcagca agaaaaaatc ctgatacttt
    12481 ttcattccaa agagataaaa cgggagtgtg acaaatcgat gtcacactcc cttattactt
    12541 acaggatagc ttttttacac accttggtaa acggtgttcc ctgctaactt ctcggtcaca
    12601 acctcttact tcactgtgat ggaatatttt gtcttgaaga tttcatgggc acctagttga
    12661 ttgatcccta ctttttccat caagtttcca ctactgttca ccgtgtcagc gattccgcac
    12721 cacggttcga tacaaacgaa aggcgcttct tttggataag gagaccagat ccctacataa
    12781 ggcatatctt tatagcttaa agttacgcta tgggaagatt tttcacttcg aatggcgtac
    12841 gcatttagac cccttgtttc aaaaaccaag gcatcttgct caaaaagttc acgattcaaa
    12901 gccagattcg tatttgtctg acctaacgtc ttttgatcca tatcgatgaa tgggcctttc
    12961 aatggtaggc gtaatcttga tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac
    13021 aatccttctt caagaggtac attgaaagca ggatgtccac cgatagaaaa gaacatttct
    13081 ttttcaccgg tatttgccac ttgataatga accgtgatgc cgtctcctcc taattcatag
    13141 ctgatcacta attcaaaatc aaatggatac ttttctaatg tctccggcgt actccttaag
    13201 atgaaactag ccatttcttg tccatgttca atgacttcaa aatccatatc ccgagcaaat
    13261 ccgtgttgcc ccatttgata tgttttccct tgataaacat actgatcatc ttttaaacgt
    13321 ccaacaaaag ggaataaaac cggtgcatgt ctgccccaat acgctggatc accttgccag
    13381 atatactcta aaccatgttc ttttgatttc agactgatca attccgcacc gtgttcagcg
    13441 atttcggcga tcagctggtc attttgaata ataacagtca ttgccattcc tccaacttcg
    13501 acttactttt taaaatggga aggctggaac aaacatttct atctgcccca gtccccttac
    13561 ttctaataac tgtgttttgc taactcgtct ttaaaacggg aattcaatac ttttaaataa
    13621 gttcccttca tgcctaacga acgagattca atgattccag ctgattccaa ttttcgtaaa
    13681 gcattgacga tcacagaacg agtgatgccg atactatcgg caatattaga agcagtcaga
    13741 cgaccttcat ctccatccaa tgcttcaaag atagcttgta cagccttcaa ttcgctataa
    13801 gataatgtgt tgattgccat ttggacagcc gttgcactcc gtacatctgc ttcgatgctt
    13861 cgagacttct gataaaggat ctgcataccg acaaccgtag ctccatattc tgctaagact
    13921 agatcgtcat ctgcaaacga ttgttccata cgagataaga taatcgtacc caaacgttcg
    13981 ccagctccaa agatcggaac gacagtcgtc agaccaaatg gatatttctc tcttgactct
    14041 accggaaatg cagtaatatc actagtaatt gggatattag cttcagtttt catcaactgt
    14101 gccactgtat cagtataaga ttcaggaaat tgtttctcaa caaacattgt tttgattcgt
    14161 tcattgttca catcgtgacg ttcattataa cctagtaata ctccgtcact tcctatgata
    14221 taggtgttgc tgtcaagaat accacctagt gtgacagcca ttttatcgta aggcaaatct
    14281 gcttgcatgt caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt
    14341 agcaagtctg tcattgtcgt cctccgattt tttataaaat atagcgactt aaatcttcat
    14401 ttgccgcaat tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt atttctccca
    14461 tttgcatatc tggtgcttca aataatagat cttctagtag tttttccaaa atggtatgca
    14521 gtctgcgtgc gccgatatta tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc
    14581 gttcaattgc ttcttttgta aacgtaactg acacattttc tgtaccgatc aacgcgacgt
    14641 attgttttac taaagcatta tttggttcgg tcagaatctt cacaaaatct tcagctgtca
    14701 aatcatctaa ttctacccgt atcgggaagc gtccttgaag ttctggaatt agatcacttg
    14761 gtttagacaa atggaaagct cctgaagcaa tgaataggac gtgatctgtt tggatgatcc
    14821 cgtattttgt gtttacttgt gaaccttcga caattggcaa tatgtctctt tggacgcctt
    14881 ctctggatac ttctccagat tgctgtgatt tagaagtgat cttatcgatt tcatcgataa
    14941 aaataattcc actttcttcc gctaatttga ttgcctcact atggatatca gcatccttga
    15001 ctaattttgc tgattcttcc ttgatcaata actctaaagc ttctttgact gttaccgtcc
    15061 gttccacttt tttcttcggt gtcaaagcac ctagagtttc atttaggtcg atccccattt
    15121 gctccaatcc attgttcatc attggtgcag gtttcttttg gtcttcaact tcaatggtta
    15181 cttcacgact atccagtaaa cccttttgta gttgttccaa gactgtacgg cgattgattc
    15241 gaatatcttc tgtcacttct tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa
    15301 gattcatcat ttgttcaaat tgattagtgg attgtttctg ttcttttttg ataccaggta
    15361 ccaagatttt taccaaacgt ttttctgcat tttttttagc acttgtgtat acacggctgt
    15421 actgctcttt ttctacgatt tggatcgcat tttctactaa atcccgaacc attgattcta
    15481 catctcttcc gacataaccg acttcagtaa attttgttgc ttcgactttg acaaatggtg
    15541 cattgacgat ttttgccaaa cgtcgtgcga tttctgtttt accgacccct gtaggtccga
    15601 tcatcagcat attttttggt gtaatatctt gttgcatttt ttcatctaac tgcaaacggc
    15661 gatagcggtt acgcaaagca acggcaacag atttttttgc tgcgtgctgt cctacgatat
    15721 attcatctaa ttcacttacg atttgtcttg gtgtttgttt taattcatac atatctactc
    15781 acctcttaca gttcttctac gataatatta tgattagtaa agatacagat atctgcggca
    15841 atatttaacg cattttctgc aatttctttc gcactcatgt cgttttgagc atagtttttc
    15901 attgctcggg ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa gataccatca
    15961 tctggtgtga taacttctcc tgtacctgaa acaagaagca tttctttatc attcattacg
    16021 atcagcatcg cttcaagttt ttgcatagat tgctgtgtcc gccattcttg agctaattcg
    16081 acagctgcgc gttgtagatt cccgttatat tcgtttagtt tgccttcaaa tttttcttct
    16141 aaagtaaacg catctgcaac acttccggcg aaacctacaa caacttctcc attgtagata
    16201 cgtcgtactt tctttgcagt gcctttcatg acaacagatt cacccattgt tacttggcca
    16261 tctccagcca tagcaaattt cccgtctttt tcgacggcac agatagtggt tgaatgaaat
    16321 ggtgattcga ccatgtttgt ttcctcctaa atttgttctt ttccttattt ttacgctctt
    16381 ggatggaaag aacgatagtt tttttgtaaa ctttcttttg ttacatgggc atagatctgg
    16441 gtagtcgata aattggcatg tcctagcaat tcttggactg tccgcaaatc tgccccatta
    16501 tttaataaat gagtagcaaa tgtatggcgc agcatgtgag gatggatatc actatccaaa
    16561 ctgctttttt tgatcatttg attcaagaca tattcaattc cagtaggcgt gattggtttt
    16621 ccatgatgat tcacaaaaac gatatcatgc tcttcgtgat attttgtcat caatacctgg
    16681 cggccttccc tgaaatattc ttgcagagca tcttgagcaa aagaacctag aggagcatat
    16741 ctctctttgt tccctttacc atggatcaac atgacctctg aatcaaaatc aatgtctgat
    16801 accgacaaac tgctacactc acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa
    16861 gcgcggttcc tcaaatccaa aggactatct cctgctacgc tgtcaaaaag tgcttgcatt
    16921 tcattttcat aaaaaaaacg tggcagtctc aaatttttct ttttgagatg aacgtaagaa
    16981 aatggatttt cttttatcac ttcttctttg agaagatact gataaaacga acgtaggctg
    17041 gctatcttgc gactgataga atttctgcta tagctaggac gatgatcagc taatgcactc
    17101 aaatagacac gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc agattcctcc
    17161 aaaaaatgtt tgaaatggag aatgtcttct tcatatgctt ctttcgtttt ttctgaatat
    17221 cctctttcga caatcaaata acgcaaaaat ttttgttccc acgtggcttc tgtcattttc
    17281 tcaccttcct tttgtcccag agaataattt agcacagcgt ttataaaaag acaaataaat
    17341 tgtcagaatt taaccaatat ttctaagttt gttcacaatt ggcgcaattc ctcacatttt
    17401 gtttgagaaa ttcttaagaa aaacgcaaga aaaagagcga gactatccgt ctcaccctct
    17461 tcatggagtt tccttactca tttgggattg cagttttttc taattctgtt tttattgcct
    17521 cattagcttt taaagctcgt tgtgcaattg cttcatatct tgcttttttg tctcgaatgc
    17581 gctctggcag ttcaggaaac agaccatagt tggcattcat cggttggaaa tgttttcctt
    17641 ccgcatgagt aatgtagtaa gccatacttc caatagctgt ttcttgtgga aactcgactg
    17701 gttccatttc ttttgctaag cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac
    17761 tttcaacata gccttctaca ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg
    17821 ttgattggta agtcggtttc agcaattctg gcgaattcat aaagctgttt cggtgcatca
    17881 caccatagcg aacgaattca gcattttcta acccaggaat catccggaaa acacgttttt
    17941 gctcgcccca tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat
    18001 tgtcttgacg cagctggata acagcataag gacgtttgcc agttttagga tcttccaatc
    18061 caactggttt cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa
    18121 ttggcataca tccttcaaaa tatttttctt tctcaaaaga tttaagttct gccacttcgg
    18181 cattgattag tgcctcatgg aaagctttga attcctcttc tgtcatcgga cagttcaagt
    18241 aagctgcttc tcctttgtca tagcgggact tcaaatatac tttatccata tcgatcgtag
    18301 ctttgtctac gattggtgca gctgcatcat agaaataaaa accatctgaa ccattgaatg
    18361 ctttgatgct ttctgctaat ggatgagaag tcaaaggtcc tgttgctact acggtgattc
    18421 cttccggtat ttcagtaatt tcttcgttta tcactgttac taatggatgc tctttgactt
    18481 ttcgggtaat tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca ccagctggca
    18541 ctgctgtttc atctgccgaa gtgattacta ccgaattcaa tcggcgcatt tcttctttca
    18601 ataaccctac tgcattagcc aaactgtttc ctcttaatga attggaacat actagttcag
    18661 caaaattact tgtgtgatgc gcctcagtcg atttgactgg acgcatttca tataaacgga
    18721 cagggactcc tgcttgtgcg atctgccaag cagcttcact tccggcaagc cctgctccaa
    18781 caacatttac cattttcaat gaaagaacct cttttctatt atttttggat gtcttcttcg
    18841 taatctccat ttacacagac tacttgttta ccgcctttga ctttcttctc gaccagatat
    18901 ccgtcacatt ttggacaatt tctgcccact ggtttatccc aagaagtgaa ttcacattct
    18961 gggaaacggg aacaaccata gaatagtctg tttttcttag attttcgttc aatgacttgt
    19021 ccttcatgac atactgggca agtcacaccg atttctttta cgattggttt cgtattccga
    19081 caatcaggaa aattactgca agcataaaac ttcccatatt tccctaattt tatcaccatc
    19141 ggatgtccac agacatcaca atcgaatcct gctggttcat ctttgatttg aattttttcg
    19201 attttttctt ccgcattcgt cagttctttt tcaaacggtt tatagaaacg atcaaccact
    19261 ttgacccacg cttccgtgcc ttcttcaatt ttatccaaat ctccttccat ttctgctgtg
    19321 aaatggatat cgacgatttg agggaagaat tcacagatta atgaattgac aatttctcct
    19381 agttcagtcg gctcaaatct tttttgtgtc aattttacat agtagcgtct ttggatcgta
    19441 tcaagtgttg gtgcataagt ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc
    19501 aacgtagcct cactaaaacg tgctggtggt tgagtaaaat gttgttttgg ttcaatatca
    19561 acagattgga ctacatcgcc ttcttcaagt tctggaagaa tattttcttt ctcttctttg
    19621 ccgtcatctc taccttccac atacacttgc atgaatcctt tgaatttgat ttttgaaccg
    19681 ttcgcaatga agatgacacc attttgttct aatgtcactt tcattgtatc taaaatagct
    19741 ggtgtcatct gactagccac aaaacgtgac cagatcaatg tataaagttt caattgatct
    19801 ttatctaaat attgtttgag ttcatctggt gtacgaagca cactcgttgg acgcacagct
    19861 tcatgggcat cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg tctagaaaat
    19921 tcttttccat acgtatcttc aataaatgct gcggcctctg cttttgctgt atcggcaatc
    19981 cgggtagaat cagtacgcat gtatgtaatc aagccgactg ttccttgttt acccaaagtg
    20041 attccttcat ataattgctg tgctaccatc atggtttttc ttgttcgaaa gtttaactta
    20101 cgggcagctt cttgttgcag gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc
    20161 tctttctttt ctactttttt aacttcgaag tctgttccat caattctagc agtgacttct
    20221 tttacacttt gtgcatctgg taatttcttt ttcttaccgt cgattcccca gaaatttgct
    20281 ttgaatttct tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc ttctggaaca
    20341 aattctcgga tctctttttc acggtcaata ataattttta aagcgacaga ttggacacgt
    20401 cctgcactta accctttctt gaccttacgc cagagaatag gactgatcga ataaccaacg
    20461 attctgtcta aggtacgacg agcttgctgt gcatctacta aatctacatc gatcgttctt
    20521 ggttccttaa aagctgcttt gactgcgtct tttgtgattt cattgaagac cacccgattt
    20581 ttttctttca gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc ttctccttct
    20641 ctatccgggt cacttgccaa gtaaactttt tgagcttttt ttgctgcggc tttcaggctt
    20701 ttgatgacat cgccttttcc gcgtatagaa atataatggg gttcataatt gttttcaaaa
    20761 tcgataccca ttttactttt cggtaaatct cttatatggc caacactggc cattacttta
    20821 tagtttcgtc caagatattt ctcaattgtc ttggcttttg caggtgattc tacaatcact
    20881 aaatatttat acgccattac tgaatggact cctttccatt gtcgtgttcc tactattaaa
    20941 agaggattca tacaaatcgt agctaatcat atccattcag aaaacgtttg tcaagaaaag
    21001 caaatgaaaa gtatggtgtt attctttaaa ttctgccaag atatcttcac ccgaaaccgt
    21061 gcaaactgca ccgtcttgga tcaaatgatg acatccggta gaatgttctg ttaaaatatt
    21121 tcctggaaca gaaaaaactg ttcttccaag atccaatgct tgctgtgctg ttatcagaga
    21181 gccgcttttt ttcttggctt caaagacaca tgttccttta gacaaagctg cgataatccg
    21241 atttctcatt ggaaaacaat gctttttcgg gccggattga ggaggatatt cactcaaaag
    21301 aagatgatca tttgcaatca cctcttgcaa gtcacgattt tcagcaggat agacttgctc
    21361 tatccctgtt ccaatgactg cgattgtccg tccaccatga gagattgctg cttggtgacc
    21421 gtatgtatct atccctttcg cgcagccact gacgatcaca tatccagctt ctatcactgg
    21481 ttttagtaaa taataaactg tttttttggc taaagcagaa gtgtgtcttc caccaacaac
    21541 gcttaacatg ttacaagaaa gtaaagatag atcaccatta taaaacagta caattggcgg
    21601 ataggtcaag tgaagcagct gagggggata tttgggatca aagcaagtga taaacgactg
    21661 tttcgattgg attttgcccc agttagcgtg aatttcctgc caacttttgc gaaacagctc
    21721 ccggtgtttg gttattttac ctatcttaat gatgtcttca aatccgattt ctttataagg
    21781 atactgcatc atccagttag caagtgccca ttttccttga ctactgattc cttttgtata
    21841 agcaagtttc aataaatttt cttctatttg atacatcata aaaccgcctt tcgtctctct
    21901 ttctaataag atacgtaaaa gcggctgttt tttattgtaa atattttttt acaggtgcaa
    21961 aacttttacg atggattggc gtaattcctt gtcgttcaag tccttctaag tgttcctttg
    22021 ttccataacc tgcattatgc gagaaaccat atccaggata aagcacgtca tattctttca
    22081 tcaattggtc tcgatatact ttagcaatga tacttgcagc agcaatcgac acagatcgtg
    22141 catccccttt gattattttt tcctgctgaa taggcaaatc caaggtcatc gcatcgatca
    22201 acaaatagct tggtacaatg gacaattttt ctactgcttg gatcatcgct aatttacttg
    22261 cttcataaat attcacatca tcgattgtct gctgatcaac gatcccgatc ccgattgcca
    22321 gggctttttc ttgaacttgt tgataaagaa gttctcgctt tttctctgat aattgtttgg
    22381 agtcattcag ccctaatatg atctcatttt tcggcaagat aactgcagct gccacgacag
    22441 gtccagctaa tggtcctctt cctacttcat caatgccggc aatccattgg tgaccttgtg
    22501 cgtacccttg cttttcaaag caaaacattt cttcatattt ttccttaatt aatgcttcct
    22561 ttttcaatcg tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt ggatcatttc
    22621 ttaatgattc tagtgtagga tcatctattc tctgtatgct tttcaatcgt tctttgatac
    22681 tttggatgga ttcagtcatc acttttttgc tccatttctt cgcaacgatc cagtgtgtat
    22741 cgtccaagtt ttccttgacg aatatcatgc acgactaatt cgctgccacg atcatagtct
    22801 tctttatacc cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa tttttcttct
    22861 tgctcgattg ataaaccata acgatcgctg attcttcctg aataatatcg ggcaaaaaag
    22921 tctaatccgt aaatcgctaa atcatctaaa tggacaagct ggtctttgat cgcgcctgtc
    22981 agcgctagtt ttttgccgat ttcttcatct tcaaatttgg gccataaaat ccctggtgtg
    23041 tctaacaatt ccaactcatt acctaaacga agccactgct gaccttttgt tacgccaggc
    23101 ttatttcctg tctgtgcaat tttcttgcct actagtcgat tcatcaacgt agatttccca
    23161 acatttggaa tacctaagac cattgcccga atagcgcgag gcttgatccc ccgagcagca
    23221 tctcttgccc gtttttcttt caatgcttct ttagccgcag gtactagacc tttaattcct
    23281 ttactttctt gtgccgtgat agctaatgca agatggcctt gtttacgaaa atattcttgc
    23341 cacagctttg tctgttgtgg atctgccaga tccgctttat tcaataaaac aaggcgaggt
    23401 ttttgctgaa tgatctgatc tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct
    23461 accaattcaa atacgatgtc cacatatttc attttttcag aaacttctcg tcgggctttt
    23521 gccatatgtc ccggaaacca ttggatggtc attgttctct ccattccttt cataccggaa
    23581 tattccccgg ctttacttgt ttttttcttt ttcaatataa attcactttt ttattataaa
    23641 gaaattccat ctattagtta agtgaaagtg aaccagtcct cattctatca catcttcact
    23701 tttttgatga tgcaaatttt tattttggtt tctatatatg aatctaaatt tttgacagcg
    23761 tttcctaaat aagagatgat agacgaacaa aacagaaagg agacgaaaca aatgaatagt
    23821 atggtttttg ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag
    23881 ctgggtttta aaaaaaatga agaattttct actgaagaag ctggttcaat ggtatgggat
    23941 gacacttttt ggataatgct tctctctcat gatttttata aaaaatttat aaaagataaa
    24001 gaaatagctg ataataaaaa aacaagtggt gctttgattt cgtttagtgt tgaaaatgcg
    24061 gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt ccacgaagta
    24121 gatatgggaa tgcctgaaga agaaatgttt tcattagaag tccaagatct agacggaaat
    24181 acattagaac ccgtatggat gaagatgtaa gcagataact aatctgacgt tttttataac
    24241 aaaaaaacaa aggcggatga tacgataagt attctccgcc tctatttttt ttattcgtac
    24301 aatctctttt ttacagttta taaaagcagt cttctcaccc tttttttaat tgttagatgc
    24361 cagtgtatgt aaatcaccaa tgtgtcatga cacattggtg attttatggt atccttttat
    24421 tattaactga caaaggagag aatattttga acactttgat tgcaaaagac gtttttagat
    24481 tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct tttattagcc cttgatgtca
    24541 tcttatacaa gatagcgatt ggtcctgctt attttcaaat cacattcgga tttttaagta
    24601 tggcgcttat gggttatcta tatggaccga tctgggctgg tttgcttgaa atgttgtcga
    24661 acactttaaa tttcacgata tttggttcag gaacgtttca agctgctttt cttatcacgg
    24721 ctttccttgg tggatttatt aatggcttat ttttatatca taaaaagata aacctgcctt
    24781 ccgtcatcgc tgcccaatta ttgattatga tcccagtcag cctttttatc aattcttggc
    24841 tgatgtctgt tttgtttggt gcagatttca aagtaatttt tctaggtaga ttcatcagga
    24901 atatgatcca aatccctatt caaataatgg ttgtttattc atttatcaaa actttagagg
    24961 ctagaaaagt gtttaaacga gctgtttgat tttacgtact aaggtagtaa actgattttg
    25021 gattaaacaa taaagcgtgc agtctcaaca gactgcacgc tttattgttt ctcactggat
    25081 ccattttatt tcatcaagtg gataatagac aaatcttgct ttacctagaa tatcagtttt
    25141 attgatcgtg ccaaacgaac gactatcttt tgaaactctt cgattatctc caagtacaaa
    25201 ataattatct tccggaagct tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt
    25261 ataggatgca tttgcatgat ctgatttcag gtttttagtt agataaggtt ccttaactac
    25321 tttcccattg atcttcaatt gatcattttg gtagctgaca ttttctcctg gaagaccgat
    25381 tactcgtttg atataaatcg tgccatctgc taattgaaaa acaacaatat caaatcgtct
    25441 gatctccgaa aatttttcca tcacaaccat atcaccttgc ttcaacacgt tttccattga
    25501 attgccttcc actggtaccg ggatgaataa aaatccccgc aacatgaacg caaggaatac
    25561 agaaacaagt aagtatttga atacaagcca gaagcgatta atatagcgtt gtttttttgt
    25621 caatctctat caatccatcc ctcttttttt cattctttct ttattataag aaaaatatca
    25681 aaaaaatgcg actagatagc tgaaataaaa ataattcttt caattaggct attggacaaa
    25741 aaactttttt gatcataaag tacaaaacta tctcttatat gtcatgatta ttttctaaat
    25801 atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca ttcagttcca
    25861 gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg tctttaccgg
    25921 cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat
    25981 aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa aggcagcccg
    26041 gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt gatggtcttc
    26101 ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct gttcgaaacg
    26161 tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt cttctgtcaa
    26221 ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga aagcgtcagt
    26281 tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca atgttggatc
    26341 atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt gttcataagt
    26401 cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac caggaagcgt
    26461 gtcgaaaggc tggatcatac accagaagca gcctcctgca aatattgctc gttccattat
    26521 gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc ttgctttcag
    26581 tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag gtatcttttg
    26641 gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct gctttaatat
    26701 acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga tcatctgtat
    26761 tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc acatctttga
    26821 ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca tttccgtttt
    26881 ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt acttcgaaag
    26941 taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca ctatcttttt
    27001 gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt gaattgatcg
    27061 ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt tctggttccc
    27121 gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc ccaagaagga
    27181 taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat acgtcagatc
    27241 ttgttttcat gtttattgat catcctcctt ctctaaccat tggttttgtg tctcgatcag
    27301 ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat ggaaatgatc
    27361 ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc tagttgtttg
    27421 atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa gcaaatcatt
    27481 gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc ctttgttcca
    27541 attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa atggattgta
    27601 gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt ccaatagttc
    27661 tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa
    27721 taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg tgataaaatc
    27781 agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta agatctggtc
    27841 acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat tataacggtc
    27901 ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat ctcctactcc
    27961 ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat ttggatcttc
    28021 tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag cagctaaacc
    28081 tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt
    28141 taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa aattttatca
    28201 aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata aagaaaaaga
    28261 gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa tctacataat
    28321 acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc gtatgcagta
    28381 cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct tgcgccaatt
    28441 cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct ttcagttcat
    28501 ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta gttacaggga
    28561 ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta gacagcaaac
    28621 cagttgttcg gctgattctt ccacctttta ccaagttatc tagtgtggat attccgatgt
    28681 ataattttgt attgtcttgt acttttttga cttcttcaag gattttctct actgttttac
    28741 cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg tcagtggtat
    28801 cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt gctgcttcta
    28861 ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg tcttctccta
    28921 gctcatcata catcgagatg aattctccaa ttggtggttg gcttgttttg ggcagatttt
    28981 ttgctttcgc catcatttcc ataaatcttt cgccgtctag ttggtcatca tcgggatata
    29041 ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca tcgcgtacat
    29101 ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt attttctctc
    29161 tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca atttctattt
    29221 ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg agccgttttc
    29281 atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc
    29341 acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt
    29401 gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt
    29461 cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg
    29521 ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt
    29581 tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt
    29641 gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa
    29701 gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt
    29761 tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg
    29821 ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg
    29881 tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa
    29941 aaataaagct atggatggga cagctctaaa taacagctag tctatccata gcttttttat
    30001 atcgtcaatt cgcctgtccc gctttttaca ggattctcat tacgctgata cgtttcaaat
    30061 tgatgcggat aacgattttt ttcatccatt tctccttgac tagtattgat cagtgaccaa
    30121 tcagaccagt caacaggtgg aaaataagca tctccatcga atacatgatg gatgactgta
    30181 cggtataata tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc tccagcgatg
    30241 aaaaagatac cgtctgtttc tttttccaga gcaagcacgt cttccacact atgcatgaca
    30301 tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc
    30361 aacggacggc tacccatccc ttcgaatgtt tttcgtccca tcactagtgt atttgcttct
    30421 gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc aaggaagacg gtcttctttt
    30481 ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta acattttctc atcctttctg
    30541 attctaaaca gcgataggag ctttgatcgc cggatgtggg tcatatcctt ctaattggat
    30601 atcttccatc tcaaaatcaa atactgactg cttttcctga ttcaaaacta aagtcggaaa
    30661 tgcgcgtatt tctcggctca attgttcttt catttgttcg atatgattga tataaagatg
    30721 agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat gtgcaattag
    30781 gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg cctagaaaca catccccact
    30841 gcgttgatac aattggcagc ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg
    30901 acaaggaggc aaagccatgc taggaacatc ttccggattc catgcagaaa cgatcaatct
    30961 tctagaatca ggagtctttt tgattgcttc gatcacttgt ttgatttgat cgataaagcc
    31021 accgtctttt gtttcccagt gacgccattg atagccgtag atgtgtccaa gatcgccgta
    31081 tgtttcagca aaggaatcat cggttaggat cttctcgcaa aaaagcttgc tctgcttttt
    31141 atattcttca ttgaattcag gatctgtcaa acatctatgg ccaaaatcag tcatatctgg
    31201 tccattatag tctggacttt tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt
    31261 tttattttgt aatagatagc ggatattcgt atctcctttt aaaaaccaaa gcaattcgct
    31321 tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc
    31381 aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg cctgttctgt cttctttacg
    31441 atgcccttct tccaacaatt ttcttcctaa gtcaagatac gcttgttcca tcctttttcc
    31501 tcctaatctt ctacccattc actcaagtat tcccaccgtg ccattttttc ttcaagttgt
    31561 gcttctgttt cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt atctccttga
    31621 tgattcatct cttcttggag caatgagatt ttttcttcaa gttctgcaat ctcatcttca
    31681 atcgtttccc attctttttg ttccatgtaa gataatttct tcttttcagc aggttccttg
    31741 ctctgtattt ttggtttcgc tttttcagca ggctgctggg tctctttttg cttcagtaaa
    31801 taactggaca tgtttccgta ataaagttct tgttttccgt ttccttggaa tactaataac
    31861 ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac agcacctgga
    31921 aaacttctaa aataatcttc caaaatcgtc aatgttgcta tgtccaaatc attggtcggt
    31981 tcatccaata ataaaacatt aggttgctga atcagcagtt tcaacaaaaa taaccggcgt
    32041 ttttctccgc ctgaaagttt gcctatcagt gtcccatgca taaatcttgg aaataaaaat
    32101 cgttcaagta attctgctac tccgatttga ctgccatctg ctgtttttgc ttcttcagca
    32161 gcttcttgta agtaagcaat catccgttta tctggtgcca tttcttcatt ttcttgtgta
    32221 taatacgcaa gatgaaccgt ttcaccgata gacattgtac cgctatccag gggaattctg
    32281 ccagctagaa tattaagcaa tgtcgatttt cccgcaccat ttttaccagt aatacctagt
    32341 ctttcccgag attgaatcaa taaatctaaa tgttctagca gtgtttggtc atttatacgg
    32401 taactgccat ctttgatttc cagtactttt ttccctaaac gttgtgtcgc taggttcagc
    32461 tctaaatcat cttcttggtt tacttggtac aagttctcct ttagatcttc gaaacggttg
    32521 atccgtgctt gctgcttggt gccacgtgcc tgtacgccag ccctcatcca agctaattcc
    32581 tgtttataaa gccgcttgcg cttttcttct tgttctactt ctacacgatc acgttctgct
    32641 ttttctaaga cgtaagcttc ataatttcct ttatactcgt ataattttcc aaatgaaagt
    32701 tcgaagattc ggtttgctac tctatctaga aaataacgat cgtgggtaac catcaatagt
    32761 gcgccacggt attgtttcaa atagttttcc aaccactcga tcgcttcata atctaaatgg
    32821 tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg cgccagacta
    32881 actcgttttt tctgaccacc tgataaagta ctgatttttt tatctaattc actgattcct
    32941 aatttttgca aaataatctt tgcgttggta tctgtagtcc aagcatcttc tttattcatt
    33001 tgttcttctg ctttagcata acgtttttgc acatcagggt cttctccgtt ttcccctagt
    33061 tcgatcaatg cttcctcata tgctcgaaca gtttggatca aggggctgtt tccttgaaag
    33121 actgcttgga gaacagttaa ttcatcggaa aattcagctg cttgagaaag ataaccaata
    33181 cggtaatcag ttggataaaa cactgtttgc cgatcaccgt cgctgctatc gataccggca
    33241 ataatattta agagacttgt tttacccgtc ccgttcgtcc caatcaaccc gatacggtct
    33301 ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt tttcaccata agtttttttg
    33361 aggtcagtta cttttaattc tttcaacatg ttccctcctt atcttggata ttgtatcata
    33421 aaaatagtga acttcaggaa aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa
    33481 gaacaggacc gattcgatcc tgttctgata tgttttgttt tttataaaac ttgaaaattt
    33541 ttaccttttt attttattgg actcgttttt tttgactttg ctcgaaacgc aaatcggaaa
    33601 gaaaagttat cagatggttt gttcatcgta taagattcta acaaattgat attcctgcga
    33661 tagctgttcg cagaaaacgt cgtgattttt tcagcaactt cgtattcatc aatgattttt
    33721 ctttcaagat aataacctct tagcattttt ttcgtaaaca gactatcttg ctggatcatc
    33781 cacgaagaaa tcaaattccc cgagttcatt tttattcctt caagtttgcg ttgctggata
    33841 aaaaattgaa ccatcgtttc atcataaata tcagtcaagt tatctaaact ttcaataatc
    33901 aactgagtat ttttttgata tgtctctctg atttcttgga tatcttcatc actaataaat
    33961 gtctgacgat cagcatatct tctttgccaa aacattttcg gatggagaac gattcgcatt
    34021 aacctacgga caacaatgaa ccaaaagccg ataaaagaca aaatctggct tgtgatagaa
    34081 tgttcgaatc gagaaagaaa acgggagtaa aaacgataac cattagaaga aagcttgccc
    34141 gaacgataac tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct
    34201 tgtacttctt gcttttcatc atcggttaaa tcttccaagt acagatcacg tatccgttct
    34261 tgataatttt ctatcactgc ttcagtggct aaaaattctt ttgtattacg atcaatttcc
    34321 tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc
    34381 gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga caatgatccc tatgaccaaa
    34441 gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag accgctggta aaacatcata
    34501 ttattgattg aaaatggcaa aataaaaatc gtagccaaac tgactgttcc ctttacaccg
    34561 ccaaatgtta agatcaagat ctcgttcaac tgttttttga actttttact gccatctttg
    34621 aatacataaa aaagactgat agaaatgaat ctgatcacaa aaagcatgac agagattaat
    34681 acgataatcg ctaataaaag accgtttgga tataaaccgt ctccccatac tggcgagaac
    34741 acttgcgtca attcgattcc taaaaatata aatactaatg cattcagtgt aaaagcaata
    34801 gttgtccatg tactgtggga caagctcgac aattctgcat caaacacaga gattttccta
    34861 aaaccagaag cttggagaat acctgctgcc actgcagcaa tgattccaga tacatcaaat
    34921 gcctctgcca atacataggc tagaaaagga agcaaaagtt cgatcagcag ataccctgtg
    34981 acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc
    35041 aaaaagccga tcaaagctcc cccaatacta gaaactacta gactcatccc cgcgtttact
    35101 gctgaaaaag atccggtgat cagcgcacca agagcaaact ggaaggcagt aacaccagat
    35161 gcatcattca aaagaccttc tccttccaaa atatgcatcg cctttttagg gatattcact
    35221 cgtccagaaa gagaactgac tgctaccgca tctgtcggac ctaacgctgc tccaaaagca
    35281 aagcaggcag caagtgggac acttggcaac aggaatgata acgtcgcacc taccccgaca
    35341 agggtcaaaa tgactcctcc aaatgccaag aacaaaattg tcccgaaatt tttgagaatc
    35401 gaagaaatat ctgctttttc tccttctcta aataacaatg gtgcaataat cataactaag
    35461 aacaattcag gttcaaaatc gattgattgt ccccactctg tcagacctaa taccactcca
    35521 aggaaaattt ggatcagtgg agctggaatc attggcaaaa tgcggctagc tatgttcgat
    35581 aaggtgatcg ttactgcaaa tacaataact aactcagcaa attccatcgt ccatcccctt
    35641 tattttttat tttgtgtaag catttttaaa aattgttctt ttttctttaa aattttggtt
    35701 tcgactactt caacctgtgt ttggttcccc tctaattcac actgtacacg ttttgactcc
    35761 aaacgaagca gatcatgttt cagacgttcc aactgacgat acatcaatat atttctttta
    35821 cgcaacttac aaagtatttg acggcggtca tccatcgaaa gggcgccata gcgatcatgc
    35881 aacagttttt tttgttgttc aaaactctct ctcattttcc agatctccta tacacaaact
    35941 tccatcatta tagcaaagtt ctttttggat gaaaaatatt tcattgtttc atcgtttatt
    36001 gctagttaga aatttccata aatttttcaa cgcctcattc tattccaaat agtatcagat
    36061 atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa ggatgtttac tttcccattc
    36121 ctgcctttct tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac
    36181 agtcccttat tctgaataaa cggtgtggaa gagacagcct atcagtagta ataagacaca
    36241 tctctttcac agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac
    36301 atcttctaca cgttgatctg gattgatcac gatatacaag ttacctgttt tttcgctgat
    36361 ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat atgggaagta
    36421 cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat cttcgtattt
    36481 caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat gtgtcgcaac
    36541 tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga tttcaacaaa
    36601 ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat
    36661 atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta gcgctgtcat
    36721 ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact acctcagttt
    36781 agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac gtatctgccc
    36841 actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa tcaatttttc
    36901 tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt ctacgacggc
    36961 tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc cagtttcttc
    37021 tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta aacgatcata
    37081 ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca attcttctac
    37141 ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag ctccagtttc
    37201 tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt tagaacactt
    37261 ccacgatttc aaaaaggaac gaatctcatt ttcttttaga tccagttggc tgatcaataa
    37321 tgcccatgct tggctctctt ctttcaatgg gacggatggt aattcagcaa aacgtaaaag
    37381 tgcttcgcct tcttctttca aaccgggaca gcaaatgtag cactctgttt caataaatgc
    37441 atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca catttatacg
    37501 ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga aggtttcttc
    37561 ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc gtaaggcatc
    37621 ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt ctagatcttg
    37681 cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg cattgatcgt
    37741 aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat gatctggtct
    37801 acggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt gttcatcatt
    37861 ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg gaaaaagctg
    37921 tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg gctggttcag
    37981 caagatatca cgaacacttc cacctacaaa ataagcttca tatcctgcat tctctaattt
    38041 tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt tcatttttcc
    38101 aacctttcct gcgtgtttct tgtcctgaga taaagactca gttgcatggt gactgtttga
    38161 caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact cgtccttacg
    38221 ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa aaacttcagt
    38281 taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat caccaagttc
    38341 ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat aagtgtggtt
    38401 gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag ctaatggaga
    38461 gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca tactgtataa
    38521 agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact ttaacgcaaa
    38581 tcctagataa gagcaattga gaaaatcgaa tttccatgct acatttagga aggaatttct
    38641 aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt caaacgaaaa
    38701 acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt tggtcttgtt
    38761 tacttgaaca tcgctaatca tttagcagaa gggggagtct cagggatcac cttgatctta
    38821 agagctttat ttggtattga tccagcttat acgactttat taatcaacat cccattgatt
    38881 ttactaggag gaaagatcct cggtaaacgt tccttggcct atactgttct aggtacactt
    38941 tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa tttacagcat
    39001 gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc
    39061 taccgcatgg gtggaacaac aggcggcagt gatattatcg ctcggatttt agaacaaaat
    39121 tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt acttgcttca
    39181 ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta tgttttcagt
    39241 cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact tgtcgtctcc
    39301 aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg tgtgactttc
    39361 ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc
    39421 tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa agctttcctt
    39481 tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa gccacagaca
    39541 agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa agattccaca
    39601 tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt tatgttcact
    39661 tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt catcagataa
    39721 tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag cataaagcat
    39781 ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt cagtatcttc
    39841 ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag atagatattc
    39901 tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt tccaattcaa
    39961 acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca aaaacaattt
    40021 catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg caaaattcgc
    40081 cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat aattcaagat
    40141 ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta aaaaattctt
    40201 cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc taataatgac
    40261 agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc ttgcgctctt
    40321 ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc tggttcatgt
    40381 gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc ttctaattca
    40441 ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc catctgattg
    40501 atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact taaagtaagt
    40561 aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc ttctgctcgc
    40621 tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc gacttggtaa
    40681 ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat ttcttcttct
    40741 tgaagagctt gtcctagata tagatataac gattgataat gcggattaac ttctcgaagt
    40801 tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt aaaagctagc
    40861 tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg tacgatagct
    40921 gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat cgagattcct
    40981 gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc ctctttgaaa
    41041 cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc atcaggaagc
    41101 aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat aacttggtac
    41161 aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc cactttttcc
    41221 aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc taacgggatg
    41281 ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg ttttgcttcg
    41341 atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc tgctgggtct
    41401 tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc tgataaagct
    41461 tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac tgacgatttg
    41521 ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt atccgtctat
    41581 tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa aaagaacttt
    41641 ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc caagcaagtc
    41701 cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga gattccacac
    41761 gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat ggccattttc
    41821 ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt ctgcctctaa
    41881 ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg ttaattgctt
    41941 tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa tcattccgca
    42001 acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc tgattgtttg
    42061 atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat ttggtagctg
    42121 caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata cagggatcat
    42181 ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc
    42241 ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt tgacgaaatc
    42301 aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa tataccagtc
    42361 attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat cataatagcg
    42421 ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct gactgactag
    42481 cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa gttccatacg
    42541 catccctcct cttcttattt ttattttacc attagtaagc aaattctaga aaagataaga
    42601 gcaaagctca cctcataact tgcaaataaa taaaaattaa ataaaataca tgtgttttct
    42661 atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct gctaggaaat
    42721 aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt cttatttaac
    42781 agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg gaatttcgat
    42841 ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt
    42901 accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa
    42961 tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg aagcaacttt
    43021 ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa gagatatgat
    43081 agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag aaatattgat
    43141 taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa ggtttttacc
    43201 aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc tttttctttt
    43261 gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat aattcggatg
    43321 gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg ttcataagaa
    43381 aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt gacagcgact
    43441 tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg attgattgct
    43501 acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt ctggttcata
    43561 cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca
    43621 aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg ttcgaaatca
    43681 cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat aataattcca
    43741 cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat cccttcttca
    43801 gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc acgcatcacg
    43861 ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc tgtatctatc
    43921 attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt
    43981 ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat caaagaggat
    44041 ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca
    44101 gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc tagtccatgg
    44161 cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata gatatcgttt
    44221 ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt acttctgtat
    44281 aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct gactacacaa
    44341 atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg atccataaaa
    44401 ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg acctaaccat
    44461 tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac aatcgagata
    44521 cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg gccaacgatt
    44581 gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc acttagttta
    44641 acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat ttttattcct
    44701 tattgaaata ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct gctttcatct
    44761 cagtcacaga tacaaaatca attatttttc agaatcatcg gttgcttttt cttctacttc
    44821 ttctttcaac gcatcaccta aaatgtctcc catagtaaat ccagtatttt cttctggtaa
    44881 ttcataagac tcaacatttt ttggttcttc ttctgaagct ggtttttctt ctaaagcttt
    44941 gatactcaat gcaatacgat gttcttctgg atggatctct aatactttca ctttcacttc
    45001 gtcaccttca tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat
    45061 atggactagc ccttctactc cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg
    45121 tttaactgtt ccgtctaata ctgtgccaac tggagctttt gtatcgattt ctgaccaagg
    45181 tccaggctgt gtgtctttga ttgacaagga aacacgttct tgttcaggat tgatagacaa
    45241 cactttgaca tttacatcat cattgacagc caatacatca cttggttttg ctacatgact
    45301 atgtgaaatc tctgagacat ggactagacc gtcaattccg cctaggtcga caaatgcgcc
    45361 aaaatcagta agacgtgcaa cacgtccact gagaacatct ccctcatgga tagttgaaag
    45421 aatttctttt ttcttttctt ctctttcgtt ttccactacc gctttatgcg ataagatcaa
    45481 acgattttct gatggttcga tttcgatgat tttaaagctc aatgttttgc ctttatagtc
    45541 tgaaaagtct gcaacaaaat gatcttccac cattgatgca gggacaaaac cacgtacacc
    45601 gacgtcaacg actaaaccgc ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc
    45661 ctcttggaaa tctctttcga tatcttccca aactttttta gcatctaaac ggcgttttga
    45721 aagtagatag ctgccgtttt ctttatcttt gccgatcgta ctgataacga ctaaatctaa
    45781 tacatcaccg acttttacta cttcgctgat atcttctacc ggcaaagttg ataattcttt
    45841 tgccggaacg actccttcaa ctccagcccc ttcgattccg acaatcactt gtttgtcttc
    45901 aatgactaaa acttcgccct taacaacatc gccaactttt acttcttgta cgcttttcat
    45961 tgcgtcttcc attgactggt tgttctcttg attttgatca aattctgtca taacacttgt
    46021 cctcctaacc aacattctgc cgtaagtacg gtactgtttt tagtttaatt gattattcaa
    46081 aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc attttttttc
    46141 ttaatttaaa aaagaagata accttttttc aaaacaacag cttttattgc tgcaacgact
    46201 tctggaatcg atttgccagt cgtatcgata cgaacagcat cctctgcttg cttcagcgga
    46261 gagacgtccc ttgttgaatc ataatggtct ctttcctgta tctctttcgt cagtgtttca
    46321 agatcagtcg gaattccttt ttcttggttc tctttgaaac gtctttctgc acgctcactt
    46381 acgcttgcta ctaaaaagat tttcacttct gcattcggta atacagctgt cccgatgtct
    46441 cttccgtcca tgacaacgcc accgcgttcc cctatttttc gttggacagc aactaattct
    46501 tctctgactt ttccatgtgc agagacttca gaaacagctt ttgtgacatc tggttgacgg
    46561 atttctaaag tcacatcttt tgatgctatg aatacatgct gtccatcttc ttgttgcgaa
    46621 aaagagatag gatacttttt gatcagcgaa actagccctt cttcatctga aaaagataca
    46681 ttgttcgtaa tagctaaata agttactgcg cgatacatgg caccggtatc tgtataaata
    46741 taattcaatt cttttgctaa tatcttggca acggtacttt tcccagatga agcaggcccg
    46801 tcgattgcaa tactgatttg tgtcatgata taactccttt ttctcagaaa aaaagccgca
    46861 aagcgcggct attattattt aattcttaat tcttgaccag ggtaaagcat aaagttgttt
    46921 ggatctaagc cattcaattc aaacaattga tcagtggtga tccctgcacg ctcagccact
    46981 tgttttggac cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa
    47041 ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt gataagttgc ctcacttgag
    47101 gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg ttacacctgt atcttgagac
    47161 acagtagatt cttccgcgat tgtactagat tctactgttg aagtctcagt tgactcctct
    47221 tttgttgtac ttgtagaact ttctactact gatgtcgaag tttcagaaga agttgctgct
    47281 gttgcattgt tgcttgatcc atttctgatc cagaaataag cacctgctgg aatagcgata
    47341 cataagacca gtaaaatcaa aaatagcgaa agaaataatg tatttccttt cttttgttgt
    47401 ctctgagaac ttcttgatga aacttcatca tctgtatcgt agattggttg ttcccatggt
    47461 tcttgtgttt cgttattttc tgtagattga tgtctatctt ttctgctcac tgttttacct
    47521 cctaaactct atcgtgtgta ttataccata ggaaaaagtg tcttgtccta aaaaatcaat
    47581 gttccatgtt ttttttaaat aaatttagta aaatcgttcc ccaatgaagt tgttcagtct
    47641 tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat
    47701 tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt
    47761 tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac
    47821 gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt
    47881 ttttttgcag ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac
    47941 tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt
    48001 gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta
    48061 catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat
    48121 cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact
    48181 gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa
    48241 aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga
    48301 ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt
    48361 tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc
    48421 gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg
    48481 ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac
    48541 ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat
    48601 tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca
    48661 aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac
    48721 tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca
    48781 ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt
    48841 gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa
    48901 gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt
    48961 ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa
    49021 cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc
    49081 acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat
    49141 ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt
    49201 aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg
    49261 atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc
    49321 gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga
    49381 taaaccgctg actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca
    49441 tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc
    49501 tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta
    49561 agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga
    49621 atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt
    49681 tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac
    49741 aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct
    49801 tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg
    49861 ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt
    49921 cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat
    49981 aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg tcactactat
    50041 gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata
    50101 ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac
    50161 agttcgtgat gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc
    50221 gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa
    50281 gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt
    50341 attttgaaag attcatttgc tatctatagt taattgacta gttttttcac ggaccctata
    50401 ggacccattt atattatttc aactttttca gccagttttc caatgtattg acgatcacag
    50461 tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc
    50521 caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact
    50581 gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata
    50641 aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga
    50701 ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc
    50761 cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat
    50821 ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa
    50881 agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac
    50941 caggataaaa aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata
    51001 agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt
    51061 acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta
    51121 agccaattag tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc
    51181 cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc
    51241 cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta
    51301 tttttattta gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat
    51361 actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat
    51421 cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa
    51481 cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc
    51541 actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata
    51601 tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat
    51661 tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat
    51721 cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt
    51781 ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc
    51841 aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt
    51901 accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc
    51961 ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa
    52021 cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa
    52081 taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat
    52141 tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc
    52201 aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc
    52261 atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc
    52321 gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac
    52381 aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc
    52441 gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac
    52501 tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc
    52561 atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg
    52621 ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt
    52681 caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca
    52741 gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg
    52801 acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt
    52861 gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct
    52921 acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga
    52981 gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca
    53041 gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata
    53101 ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac
    53161 atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat
    53221 ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg
    53281 ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa
    53341 aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt
    53401 actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta
    53461 cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc
    53521 gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta
    53581 ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt
    53641 gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt
    53701 ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa
    53761 ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt
    53821 gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct
    53881 actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc
    53941 agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca
    54001 aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact
    54061 gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca
    54121 accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct
    54181 ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt
    54241 tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc
    54301 ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta
    54361 tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct
    54421 tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac
    54481 aaaaataata acacgcattt tgtaaaac
//
LOCUS       DASJTM010000006        50997 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJTM010000006 DASJTM010000000
VERSION     DASJTM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.006"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..225
                     /locus_tag="U4B29_000334"
     CDS             79..225
                     /locus_tag="U4B29_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105306.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            297..1523
                     /locus_tag="U4B29_000335"
     CDS             297..1523
                     /locus_tag="U4B29_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2105307.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1611..2003)
                     /gene="rpsI"
                     /locus_tag="U4B29_000336"
     CDS             complement(1611..2003)
                     /gene="rpsI"
                     /locus_tag="U4B29_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2105308.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2019..2462)
                     /gene="rplM"
                     /locus_tag="U4B29_000337"
     CDS             complement(2019..2462)
                     /gene="rplM"
                     /locus_tag="U4B29_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN2105309.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2981..3448)
                     /locus_tag="U4B29_000338"
     CDS             complement(2981..3448)
                     /locus_tag="U4B29_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN2105310.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3568..5106)
                     /locus_tag="U4B29_000339"
     CDS             complement(3568..5106)
                     /locus_tag="U4B29_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN2105311.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5186..5872
                     /locus_tag="U4B29_000340"
     CDS             5186..5872
                     /locus_tag="U4B29_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN2105312.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6073..6600)
                     /gene="msrA"
                     /locus_tag="U4B29_000341"
     CDS             complement(6073..6600)
                     /gene="msrA"
                     /locus_tag="U4B29_000341"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2105313.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6684..7361)
                     /gene="pgmB"
                     /locus_tag="U4B29_000342"
     CDS             complement(6684..7361)
                     /gene="pgmB"
                     /locus_tag="U4B29_000342"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2105314.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7358..8488)
                     /locus_tag="U4B29_000343"
     CDS             complement(7358..8488)
                     /locus_tag="U4B29_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2105315.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8488..10671)
                     /locus_tag="U4B29_000344"
     CDS             complement(8488..10671)
                     /locus_tag="U4B29_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2105316.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10668..11693)
                     /locus_tag="U4B29_000345"
     CDS             complement(10668..11693)
                     /locus_tag="U4B29_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2105317.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11738..12565)
                     /locus_tag="U4B29_000346"
     CDS             complement(11738..12565)
                     /locus_tag="U4B29_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2105318.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12562..13629)
                     /locus_tag="U4B29_000347"
     CDS             complement(12562..13629)
                     /locus_tag="U4B29_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN2105319.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13655..14491)
                     /locus_tag="U4B29_000348"
     CDS             complement(13655..14491)
                     /locus_tag="U4B29_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2105320.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14488..15357)
                     /locus_tag="U4B29_000349"
     CDS             complement(14488..15357)
                     /locus_tag="U4B29_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2105321.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15427..16710)
                     /locus_tag="U4B29_000350"
     CDS             complement(15427..16710)
                     /locus_tag="U4B29_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2105322.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16724..18409)
                     /locus_tag="U4B29_000351"
     CDS             complement(16724..18409)
                     /locus_tag="U4B29_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN2105323.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18684..19709
                     /locus_tag="U4B29_000352"
     CDS             18684..19709
                     /locus_tag="U4B29_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2105324.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20304..23978)
                     /gene="rpoC"
                     /locus_tag="U4B29_000353"
     CDS             complement(20304..23978)
                     /gene="rpoC"
                     /locus_tag="U4B29_000353"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN2105325.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24036..27653)
                     /gene="rpoB"
                     /locus_tag="U4B29_000354"
     CDS             complement(24036..27653)
                     /gene="rpoB"
                     /locus_tag="U4B29_000354"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2105326.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28381..29349
                     /locus_tag="U4B29_000355"
     CDS             28381..29349
                     /locus_tag="U4B29_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2105327.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29367..30272
                     /locus_tag="U4B29_000356"
     CDS             29367..30272
                     /locus_tag="U4B29_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105328.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30269..31075
                     /locus_tag="U4B29_000357"
     CDS             30269..31075
                     /locus_tag="U4B29_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2105329.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31211..32200
                     /locus_tag="U4B29_000358"
     CDS             31211..32200
                     /locus_tag="U4B29_000358"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN2105330.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32517..33041)
                     /locus_tag="U4B29_000359"
     CDS             complement(32517..33041)
                     /locus_tag="U4B29_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN2105331.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33328..35139
                     /gene="scm"
                     /locus_tag="U4B29_000360"
     CDS             33328..35139
                     /gene="scm"
                     /locus_tag="U4B29_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2105332.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35294..36280)
                     /locus_tag="U4B29_000361"
     CDS             complement(35294..36280)
                     /locus_tag="U4B29_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2105333.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36303..36869)
                     /locus_tag="U4B29_000362"
     CDS             complement(36303..36869)
                     /locus_tag="U4B29_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN2105334.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(36883..37299)
                     /locus_tag="U4B29_000363"
     CDS             complement(36883..37299)
                     /locus_tag="U4B29_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2105335.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(37756..39690)
                     /locus_tag="U4B29_000364"
     CDS             complement(37756..39690)
                     /locus_tag="U4B29_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2105336.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            39971..40927
                     /locus_tag="U4B29_000365"
     CDS             39971..40927
                     /locus_tag="U4B29_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN2105337.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            41017..42120
                     /locus_tag="U4B29_000366"
     CDS             41017..42120
                     /locus_tag="U4B29_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2105338.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            42133..43086
                     /gene="menA"
                     /locus_tag="U4B29_000367"
     CDS             42133..43086
                     /gene="menA"
                     /locus_tag="U4B29_000367"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN2105339.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(44021..45367)
                     /gene="gor"
                     /locus_tag="U4B29_000368"
     CDS             complement(44021..45367)
                     /gene="gor"
                     /locus_tag="U4B29_000368"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN2105340.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(45501..46754)
                     /locus_tag="U4B29_000369"
     CDS             complement(45501..46754)
                     /locus_tag="U4B29_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2105341.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(46760..47683)
                     /locus_tag="U4B29_000370"
     CDS             complement(46760..47683)
                     /locus_tag="U4B29_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2105342.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(47904..50396)
                     /locus_tag="U4B29_000371"
     CDS             complement(47904..50396)
                     /locus_tag="U4B29_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2105343.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(50403..50870)
                     /locus_tag="U4B29_000372"
     CDS             complement(50403..50870)
                     /locus_tag="U4B29_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN2105344.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        14722 a        10777 c         9395 g        16103 t
ORIGIN      
        1 ctatgtgctt acccaaagca attcaaaaat ttcataacta ctattttagg agatgatttt
       61 atagaatcca gagtctacct gaataaaaaa caaacctgtg catttctagg aatcgataat
      121 aatactctag ataagtggat tcgtatatat aatttcccag taataagagt tggtggtgta
      181 gttcgctttg acgtagaaga tattaaagac tttatgaata cttgaaattt tcttctcgcc
      241 gcatatacta aaattagtga aatatactgc cttacttttt ttaaggagtg aggattttgc
      301 ctacaaagct ttcaaatggg aaatataaaa ctaatctgag atatccaaaa aggtttaaag
      361 aaataacagg aattgcatca gaaaagtatc aaaaaacatt tccaaatagg caattggcga
      421 ttaaagccga aaatgatatg aagaagaaaa tagaaaaagt tttgagagag gagaatgcaa
      481 actcattaga attaaaagga aaaataacgt ttaaaaaatt ttacgaatct aaatggcttc
      541 ctagatatga attaggtcaa acaattagat caaacaggcc tcctagtgat ataaccatta
      601 gtaatacaaa agatatattt cggttgcata ttttacctat gtttggtgaa tacgcaatga
      661 attatctaaa tatcaatact gagataatat cagatgaatt aaccaaaaaa tcgaaagaat
      721 atgcaaatat aaaaatcata aaaggatatg ttcgttccat gttcgatatt gcagaaatac
      781 taaattatat cgaatttaat cgaactacta aaattattca aagtatcact gcacctaaaa
      841 aaaatgcttt agaagaaaaa agaatacaag aaggtaagca agcactatca tcaaaagaac
      901 ttactaattg gatagaagca gtaaatgatg attttaataa tcatttactt acttttcatg
      961 attacacact ttttatgatt accctgtatc ttggtgatag aaaaagtgag acttacgctt
     1021 tgcaatggaa atatattgat tttgaaaaac aaacggtccg tcttaaacat actttagata
     1081 aatatcaaag aaaaaagttt acaaaaggtc gaaaagatac cgtcattcaa gtaccagaag
     1141 ttgtaatgac attgctaagt gaatggaaat cggtacaagc tgatcaattg ttgaaattaa
     1201 aaattaagca aacgttagat cagtatctct ttacttatac taaaccatct ggagaagtaa
     1261 attgtcctgt tcatgccgat tacctaaact atcgaatcaa ttcaattaaa agaaggcatc
     1321 ctgacttagt tcatttatct ccacacaaac tccgtcatac ttatgcaact atagctagac
     1381 aaggtggagc agatatgaat caaatttcaa atgctttaac acactcagat atttctacaa
     1441 caaagatata tgtgaacaca ccagatatag ttgataaggc tgtttttgaa gcttttcaga
     1501 gaggattgaa taagtgtgat tgatatgtgt tcttaaatgt gttcttaaaa acaaaagaag
     1561 cactttccaa ataattgaaa agtgccagaa acgttgatat ataaaggata ttaacgtttt
     1621 gagaattgtg aagctttacg tgctttctta agacctggtt ttttacgttc gaccatacgt
     1681 gcgtcacgag taagtaagcc agcacgttta agagctaaac ggaagtcagg atcaacttct
     1741 agcaatgcac gagcgatacc atgacggata gctcctgatt gtccagcgta tcctccgccg
     1801 tttacgttaa caaatacgtc atatgcgcct tttgtttcag taacagcgaa aggttgattg
     1861 attacttcac gcaagtcagc atgtggaatg tattcttcaa catctttttt atttacagta
     1921 attttgccag ttcctggtac tagacgtacg cgagcaactg cgtttttacg acggcctgtg
     1981 ccgatatatt gaacttgtgc caatgaaatt ccctcctatt agattaagtt tgtgatgtct
     2041 aaaacttctg gttgttgtgc tgcatgtgga tgttctgctc caccgtatac gttcaatttt
     2101 gtaaattgtt tgcggcctaa agtgttttta ggaagcatac cttttacaga agtttcgatc
     2161 aaacgacgag aatttttagc acgcaattcg ccagcagtga ttgatttcaa tcctcctggg
     2221 tagttgctgt gacggtaata aattttgtca gtcgcttttt tacctgttaa tttcactttg
     2281 tctgcattga tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa agttggttta
     2341 tttttaccac gtagtacaga tgcaacaact gttgaaagac gtcccaatgg aacatcagtt
     2401 gcgtctacta cataccattt acgttctact tcgcctggtt tggccatata tgttgtacgc
     2461 acgttatttt cctccaattt aagttctgat gtttgacata cacttcgagt ttccggggct
     2521 ctttgtgggg caaacaatac catttaacat attacccgct aatctagtca atgtcaacgt
     2581 ttttctgctt gattgaaaaa tttatatgaa actttacttc ttttctttct tttatataaa
     2641 agggtcgttt cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa
     2701 ccccttattt tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
     2761 cataaaacat aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag
     2821 gcttacaacc tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
     2881 cggcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg
     2941 atttcacaac ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg
     3001 ctttgcccaa atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga
     3061 taaagatatc ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt
     3121 aaagatctgc tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac
     3181 gatcccagcg gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag
     3241 aataaggtga accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca
     3301 aatcatccat gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt
     3361 accaatgtgt ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta
     3421 atacttcttt ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta
     3481 aaataatagt agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc
     3541 agttcgcttt tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag
     3601 ttgagcaaat tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata
     3661 gattttctga tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat
     3721 caattcctgt gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac
     3781 agctcctaat gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc
     3841 agccatcatc tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt
     3901 gagctgattt tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc
     3961 tagaatcgcc ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc
     4021 aaacgcatct gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag
     4081 accattggca cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc
     4141 tcctttttct ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa
     4201 gatgccagca ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata
     4261 acaaaacgtc tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc
     4321 gctcgttcct acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga
     4381 aagcggcccg tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc
     4441 tgctgttttt gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg
     4501 ctctattcga atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc
     4561 aaaatatcca gttcctgaag ctgttccgta gtcgctgatc agttcgccaa acaatctata
     4621 aaagatatag tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct
     4681 gttttgcagc cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg
     4741 ccgataaaat tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg
     4801 gttatctgcc caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag
     4861 gccatgcatc gcagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt
     4921 aattacttct tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc
     4981 catcccttgt gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc
     5041 ttcatctgtg aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac
     5101 gttcattttt tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc
     5161 ttggttttga aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg
     5221 atcattttta tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag
     5281 atctcattgt gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc
     5341 gatcatcgtc caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt
     5401 gttggcagaa gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag
     5461 aaatttttcg acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt
     5521 ttacttcatg gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg
     5581 gttgcaggcc ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt
     5641 gtgccttcgc tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg
     5701 gagtggtggc ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg
     5761 aatatccgtt ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt
     5821 cattcctttt ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt
     5881 tgtgacaaac gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga
     5941 cttggagcta ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc
     6001 tcagtattag ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc
     6061 tttttcggct tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga
     6121 accttctcga taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc
     6181 ttctgcagga taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg
     6241 cccgctgtct tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt
     6301 atagtagatc actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt
     6361 cggatcggtt tgctgccaat agatttccac caactgatca taagaaataa cttctggatc
     6421 atattcgatc tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt
     6481 tggatttggt acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc
     6541 ttcaaatggt ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat
     6601 ataaaatccc ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct
     6661 tgtgacagaa aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac
     6721 gaacgtactt gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt
     6781 atgttttcac ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct
     6841 tctgaatctt caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg
     6901 agaaaaactt ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca
     6961 aaataacttg tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc
     7021 aatccgatct tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa
     7081 acggatgttt catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca
     7141 aaggctgatt tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc
     7201 tctagtgact cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga
     7261 atggacagct ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca
     7321 agaacgccat ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat
     7381 gaacgacttg aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtggctt
     7441 gctgtaaagc ttcttgtaaa ggcacagctg caggaaggat cggaaaaacg gccgaaatgc
     7501 caacatctgc tgctttttcg cttcctttac caacacttcc gcaaatagct atcacaggaa
     7561 cgccctctgg tactcttcta gcaatcccaa ctggcgcttt tccttttaga ctttgtccat
     7621 ctagacgtcc ttcgcctacg attacgaggt ctgcttgctg tacccgttta tcgaactcca
     7681 gtaaatccaa tacgtagtcg atgccagaga ctattttccc tgaagcaaat gctaacagtc
     7741 ctgcacccat tccaccacca gcactactgc ccgatgcctc gattacttga ggggcaaatc
     7801 tcatataaaa atcatgcatg gcttggtcag ccactgctaa ttcattttct ttcaagcctt
     7861 tttgtggtcc gaaaacaaaa gaagcgcctt cttttccgca taaaagatta gtaacatcag
     7921 taatgacagt gattttggct ttaatcagct caataggcac gttcgtttta tcaactgtag
     7981 caattttacc tagattttct cccagtgctt ctactttttg attatcttga tcgtaaaaag
     8041 cataacctag accttctgcc atgccgatgc cgccatcgtt ggtactactt ccgccaacgc
     8101 cgatgatgat gtgtcgaaca ccttgttgca acaattgtcg tatcagttct cctactcctc
     8161 gcgtggataa cctaagtggc tgttgttggt ttagtgggac atctttcaat ccgacgattt
     8221 ctgccatttc aaaaatcgca gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc
     8281 caaatgcatt agtaacgact gctgtttgcc gcttcaacct taaactgtct gctaatgatg
     8341 aaaccgttcc ttctcctcca tcgcctatag gtagcagatc atattcagcc tgaacaaaaa
     8401 cttcttgaaa acctgattga atggcttgtg ctgcctctaa ggctgtcaga ctttctttga
     8461 aagaatctgg cgcaatcaga atacgcatta ttttcccctc cgttccaaga tcagttcttt
     8521 ttccagctgg tattggtctc cattgacaaa aataggtaat gtttctttcg tctctttttt
     8581 tattactatt tgtttttttg tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg
     8641 aaaagaaatt ttttcccatg ctgaaggaag tcttggagaa atagaaagag ttccattcgt
     8701 tactgtaata cctgaaaaac caaatataac tgctagccat agtgctccta atgaagcagc
     8761 atgaattccg tcatctgatg aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg
     8821 gaataaatga tacgcccatt ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc
     8881 tttactcaac gatgagtcgt ggattgtttt gttttcatag taatgcaaat tcttagaaac
     8941 gattggttga gaaaaatact caggtaataa gtataacagc atgacaagat ctgcttgctt
     9001 gctgacttgg agttcattta cttcgctacg ggcataatca agtaaaatag actggctgcc
     9061 aggacgttct ttgtaagtgg tcaaatcaat aactggctta ctgagatacg tgtcatcttg
     9121 cggaatcagc tgttctttgt taggcactgg caaatataac ttatctaaaa aattttgaca
     9181 ttctttcaaa aaaagcggat cgccagcttg atatttaaca gctaaggcca cattataatg
     9241 cgccagatag ttcgtatatg cattattgtc gatatattct gtgtattcat ctggaccaat
     9301 gactctgtgt atctctaaat gatcatcgac ttttacagct ctgctcagcc aaaaagctgc
     9361 tgtttcatga atgagcgttt ttccctttgt ttctaaaaac gattgatcct ctgttgcttg
     9421 gacatactct tgaacggcat aagcaaggtc tgcactgata tgatgttctg cggcagctga
     9481 agcaacttct tgtcttgttc cggttttgat attcaatgca gcaaatttgg gtgtctcttc
     9541 ctgaccagtg tgtgcgcttt cccatgggaa aagtgcacct tgatagtgat aatctcttgc
     9601 tttttgccgt gcgccttcta atcgatcata acggtacagc agcaaattct ttgctacttc
     9661 aggaaactga tataaaaaga aaggcaggat aaaaatttcc gtatcccaga aaacatgccc
     9721 tttataacct tcgccagtta gtcccttcgc ccctacacta acacttggat cattgcgggg
     9781 agtcataatc tgtaaatggt aagcagcgaa gtccatcgct aactgatcga aagaagaatc
     9841 gccttggatc gttatccgat gctgatgcca aaaatctgtc caataagaca tcgactgctc
     9901 taaaatttct gtataactaa cgcttttctg ggcttgggca atacttttct taaacgggtc
     9961 tgtttgctga agagaggtag agagcacgat gatcttctcc agcgtaaagg acgtattttt
    10021 tctgatcttt tctgaaaagg aagcggtgat ccggcgattt tttgcttgga atacacctgc
    10081 tttattgaat ttagctgcaa tcccaatatc aattttactt tctgttgtct ggtaggatgc
    10141 actgatcaaa tcgtcaccaa atactcgcag ttctttttca accaactgct gtgtaccaaa
    10201 attggtttgc tgtccatcga tacccgtttt cacatctact tggcagtctt cggtcaacgg
    10261 cttgatgacc actttcaggg ctgcctgatg gagattattt tgatcagcaa cacgacaagt
    10321 aatgacttcc actttttttc cttgagcagt ttcaatgagc gcatagcgtt taagctctcc
    10381 catttctaaa tctagtgccc gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg
    10441 ttcaccgtcg atttttattt cccatctgat gacatctggg agattcacta actcggctga
    10501 ttctgctcct aaaggtcggt tatatacacc agctgcaaac attcccctta cttgattccg
    10561 atagtcttcc tcctggcttc ctctgatacc aaaaaatcca tttccaatgg tcatctgact
    10621 cgcatactta ttgatatact ctaaatcaaa ctgtttttct tctactttca taaggtaata
    10681 acttctcctg tttctgccga acgatataaa ttttccacca gattctggat gacatatcct
    10741 tgccagccat ctgcaatcat ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc
    10801 atacttagtt catgtgggtc attaactggc gtggtctttt gtaaggaaac cagctttcca
    10861 tctacatctt cgaatagttc gcctggatac agattagcac ctgcctgatc gccacaaaaa
    10921 gccacattca gtttgtcttc tctgatattc aaagcaaacg aagtattcag ttggatgatc
    10981 ttgccgtttt ctaattccag tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga
    11041 tcccatttcc cgaatttccc tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc
    11101 cgaacgcttt ttactttagg aaatccagta atatacatcg ctgtatcaag catgtgaatg
    11161 ccataatcga tcaaaggccc gcctccttga agttctttgt cgataaacgt tccccagcct
    11221 ggaacaccag accgacgaag cgcattcgct tctacgtaat aaatatcccc gagaacatca
    11281 agatgattct tgatcattgt tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa
    11341 atctgcccgt ttttatcggc tgtctctgcc atctttcttg cttgatcagc tgtcatagct
    11401 ggtggtttct cgcacatcac tgaagcatta gcttcgaggg cagccaatac atgttcgaag
    11461 tgaaagcgat tgggtgtaca gacactcacg atatcgggag aaatctcatt cagcatttgt
    11521 gtaactgatg tatacgcatg agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg
    11581 ctgactggat caaccacagc ggccaattct aagacatctg atcttttttg ataataagca
    11641 gcatgtactt tttctgctac ttgtccagcg cctatgattg ctactttctt catttatttc
    11701 tccctccttt gcttatttac ttattgttat tctttctcta ttctttctct aaatcattta
    11761 aatgacgcgc tgtttccaga cagtttttca agtattgcaa tgattcctga taaattttca
    11821 cttcattttc tcctcgcaaa cgacattcat ataccatata accttcatac tcattttgaa
    11881 gtagcgtttg aaaatggcga gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat
    11941 tatctgctaa atggatatga cccacatact ctttttgcgt agtcaatgct tttgaaatat
    12001 catcttcttc aatattcata tgataaaaat cagcgatgat ctgcacatgt ttcaattgat
    12061 tagagtcaat gtaatgacgc gcatccgcta acaaattgat catatggtct tggtatcggt
    12121 tcaaaggctc caaataaata accgtacctg tttcttcggc tactttatct aaataacgaa
    12181 gtgattcgct gactgcttta tagtctcctt ctttgcttcg gggtgaaacc atcggcggta
    12241 atcgataagt gaacattccc caagctgctg ggataacgat tcctttgcct ccaacctcgc
    12301 ttagtgcacg caaaatctca gcaatttgac gtacaccatt aagacgccgt tcttcgataa
    12361 agtcaccgat ccacccatcg tagccgttac aagctgtcac tactgggatc cctgtttctt
    12421 tgattgcctg tttgactgtg tcgatatttt ctattaacaa cttaccgtcg atttcgtatt
    12481 cttcaaatcc acagtctttg ataaatctaa atttttctaa aatgtctctt gggaaaaatg
    12541 cttgatcttg cgtaccgaat ttcattgttc tcctcctcgg acttgaacgc ccattttgat
    12601 actttcttct gggtgctggt ctacataacg catgtaagct tctgcgcttt ctgcaaatgg
    12661 cacaactgga tcaatgattt cttcacaatc caagtaaccg ttcatcaata attcccaaca
    12721 tgtgtcctcg attcgtttac ggttccatct tgggtaatct gggtttggtt cactagatgc
    12781 tcgagcaaag acgatttttg cattattgaa atgtgcttcg cgaccaagat taaacccagc
    12841 tggaaaaggt ttagcaaagg caacataaga aatgatcccg ccatagccga tccctcttaa
    12901 agaagcttgt aatgcggcag cactgccact cgtttcaata actgaatcta ctccacgttt
    12961 atctgtcaat tcctttagac gaaaaccgac atctgctttc ataggatcta gcgtgtaatc
    13021 ggcaccgtat ttttctgcta attctctacg ctgcgctatg ggatcgatcc caacgatggt
    13081 agtagcacct gcttttttag ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa
    13141 gattgctaca gcatcacctg ctcgaacatg tgcttcccgg ataccaccta aagcgaattg
    13201 agcaggatcg taacatactg catttttcca ttgatccaac cgatcaagtt tacgtagttt
    13261 ataattatca acaccgtttg taatcagcgt ttcacgaatg ggtccataag tgcagactaa
    13321 ctctcctttt tgatactctg tcaccaaact gcccgtttct tcgatcgtac cgacaatcat
    13381 atttcctaat tgaaattcgc caaataccac accgcgttgg cttccttctt ctcttggcaa
    13441 gaataggcgc cattcttctg aaaattcttc atcgataaat ggacttaatc ctctaaagtc
    13501 gacaatttct gttcctggtt ttggagtagc gaaaattact cggatacgta cttcgttctt
    13561 ttcgatctca cggtcttcgt aatctttgaa tccggctatt cttggttcta atgcaactaa
    13621 ttttttcatc ttttctttcc tctttctttt ctttttatcc ttttacgccg ccttcgacta
    13681 aatcaccttt aataaatcgt tcagataacg catacataat gatgactggt aaagcggtca
    13741 ccaatgaagc ggccatcatt cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca
    13801 aaccgattgg taaagtaaat ttttcggagg tcgacaggaa aatcgaagca aataaataat
    13861 cattccacga aaccataaag caataaacga agacagaaat gattccagaa atagccaatg
    13921 gtataatgat ccgaataatg atctggatcc ggctaagacc atcgatcatt gctgcttctt
    13981 caatatcagt cggaatcgtg tcaaaataac tttttagcat gaatatcgca gtaggaagtg
    14041 tttgaacgac catcgtgata atcagtgctg tattggtatc atacaaacct agaccggaaa
    14101 tgattttaaa taatggaaca acgatcaaaa taccggaaaa catataaacg gtgtaaaagc
    14161 tagcattgat tttcgagcga gctttgaagc gtagttttga caaagcgtat gccccgctga
    14221 tcccaatcac tactgcagtt acggaggcta tcaaggagat cactaaacta tttttgaaat
    14281 acatgatgaa tggaaaaatc tcagcattga aaatatcctg ataatgttca aaagtccatt
    14341 ctttcggaaa aatagtttgt gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca
    14401 tcagcatgat caaaaatgga aacaaattga cagctaatag caacaaaatc gtcgcgtaaa
    14461 ataagatttt cctgctcttt ttatttttca tttcaagtcc accttctttc gtgagagcaa
    14521 gacgaccgct gcaataatga tgaacaatag aatcgatata gctgctgctt ttccaagatc
    14581 attgaacgca aaggctgtct gatacaaata aacgcctaag atattgactt tattcgtcag
    14641 tagatagaca tctgtaaaca tatagaacat ccagatagat cgtaaaacaa tcacagttgc
    14701 tagtactggt ttgattgccg gcagtgtcac gatcttgaac ttctgccaag catttgcacc
    14761 gtccatttca gctgcttcat aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga
    14821 aataaacgca tacggatagt atctccagat agcaaacaaa acgaccataa agaagctagc
    14881 aactggctgg tcgaaccaca gcggtgcttc gttgaaaagc tgcaacctat ctactaacag
    14941 atcattgacg attccataac tattattgaa catatatttc caagcaaaaa tcaaagaaat
    15001 agatggcgta acataactta ataaaatcag tgaacgcacc gttttcctaa atctgaactt
    15061 gcggttaagc aaaatagcta cggctaatcc taaagctgta ctgcctgcta caactaagaa
    15121 tgtgtaagca actgttaaac ctaaagaacg ataaaattca ctatcactta aaatttcaag
    15181 ataattgctt aatccgataa aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa
    15241 actgatctga atattggaaa gcattggaat cagcactaaa acagctaata aaagcatgct
    15301 tggtgctaaa agtcccatgg ctaattttaa gtcagaatgt ttccttggac gattcattga
    15361 aacctccccc tgataatctg ccaatagcta tctacttttt tcgaacacca ttggcagaac
    15421 ttcttgttat ttcgtatttt tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc
    15481 gacagttact tggttcactg cttttccgat cacacctgag cttgtgatat ctcccatttt
    15541 cgtgaaattc ttttccccga ctagtccaaa gacttgtact tcatcaaaag cactagcaat
    15601 ctcagatggc agctcgccaa aagacttgat gacttcattt tctttgtaac catccagttc
    15661 gactacttgt ttattcactg gttgagcacc accaggagac atcaagaccc acttttccat
    15721 gtttttcggt tgagataaga aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc
    15781 tagaccagaa gaaatcgtca atccagatac tgtgccatag acagcttgat ctttttgtgt
    15841 tgggatagca aaaccgatat tttctgagtt ccccgcttca taaacagaag gcaaaatgta
    15901 agttgaatag attgccattg gtgctgttcc attcatgaaa gcatctttga tttctgtcac
    15961 atcattagag cctggcatcg tgtattggga taagttttga taaaacgaca atgcttctct
    16021 catcttttcc gtatcgatcg ttacctcgcc ttcatcatcc aaaacatttg ctccatttga
    16081 caaagcaaat tgagagaacg cttgctcact cattgttcct tcagcagtag gcatagcaat
    16141 tccatattgt ttattctctt tatctgtgaa atgttgagca atttttaaaa tatcatccca
    16201 attttctggt tctgaaaaac cagcttctga taatttttct ttgtcatacc aaataccttg
    16261 aacccagccg ctaattggtg cagcaatata gctttttcca tcttctgaac gaacaaggtt
    16321 tttggctccg tcataataat tatcttctcc agcttcctcg ataacgtttt ggactgccgt
    16381 ctgatcaatc agttcatctt tatccatcac tttagcaaaa tcctgactga cttccattac
    16441 ttctggtaat ttaccggaac gagctaacgt aacgattttt gtattgaacg ctgattcttc
    16501 taccggcact tgcttgattt tgatgtctgg attttctttt tcaaaatcag caaccagttc
    16561 attgatcaca cttaatcgtt cctgttcgac agaagagtgc atgaattgaa tcgttgtctg
    16621 ttgtgattcg ttagagcttt gactttcacc acaagcagta agaagaaatc cagtagctag
    16681 cagacaggta gtaacaataa tttttttcat ttttttccac ctcttatttt attattttca
    16741 gccacatata ttcatacggc tggaatatca actttcgttc ttgactgcaa tggataattt
    16801 gtccagaaaa caggtctagg taatctcctt ttggtaaaag ataacctgct gattgattgg
    16861 aaaaattatg gataactaag actttctctt ccttcgcttc gcgaataaaa ctgataaaag
    16921 aatctgctga gtccagtaag taaatcaaac tattcggatg gaaggctttt tctttttttc
    16981 gaatagatag catctgggtc aattcaccat atactgctgt tcttaatgga tctgtctgca
    17041 actctttctc aatatctgct aacaaaaatt tttgccgatt gatttttcga ttctctcctg
    17101 ttttttctac gcctttgtaa tcatttcgac ttcccagcac actttgaata taaattgccg
    17161 gcacgcctgg taacgttacc aaaaatgaat gagcagctaa aaagcgtttg cttctccatt
    17221 ccgtcgtatc tgttggagaa cttaacgcat ctagatacgt agcgtttatc tcatatggac
    17281 tggtcgttcc atctgggttt ttcttgtagt ttactcgggt accctctttc ttcagctgtt
    17341 ccactaattc aaggatcgtt ttttccggca gaatacctcg taccggattc aatccgatac
    17401 cgtcatggga ggctaaaaaa ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt
    17461 ttaaccattc tttcaactta cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg
    17521 gtagcgaaaa ctgatagacc atctgtgctt cattctcgcc attcccaaaa taactgatat
    17581 tatctttgtg tggcacattt gtctcagtga tcaataccgt tcctggagct gcttcatcag
    17641 cgattgcacg gaataattgg atgattttat gcgtttctgg caggtgtata caagaagttc
    17701 ctagttcttt ccataaaaat cccacagcgt ccaagcgaat aaatgaagcg cctttttcta
    17761 agtaaaacaa aagaatatcg atcattttca ataaaacttc tggattttta aaattcaagt
    17821 ctacttgatc ttcgctaaaa gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga
    17881 acgttgtaag taacgggctg gtccttggtc tagtcacgct gcttgtatct gtatccagtg
    17941 gcaaatcaat aaaaaaatct ttgtacgtat cattcaagtt taaatattcc tgaaaccaag
    18001 tactttcggc agacatatga ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca
    18061 tttcttctat atcttcccat gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg
    18121 agaaaccatc atcagatgta tatgggaaaa atggcaagaa atgtacaact ctaaatgtat
    18181 ctgccaaata cttttgataa aatttcttaa atgtacgcaa agttggttgc tgctcttcaa
    18241 aaaattgatc cccataagta atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg
    18301 cctggccttt cttctgctga taaacactga ctttttgttc aatcatagag gcggttttct
    18361 cagacaaatc tgagtcgcga taaatcgctt gtaaaagttt tgtatacatc ggattcccct
    18421 ttcttttttg ggagcgttcc caacgatttg atcatattat ggaaaacgct tactgtcaat
    18481 agcatttttt taaataattt tgatatacac aaaaatacaa ataagaaagc ttatgtatca
    18541 ataattaata gcttcttaaa aaaatatggt ctctcatgat catgcaagct attcctaaat
    18601 tttcaaaatc atagacaaaa aagaatgtct attgtactat ggaattattc taaaacgctc
    18661 ccaaaaagga ggtgcatata aaattgaaac ctactattta tgacattgcg aaaaaagcta
    18721 atgtctcaaa atcaactgtc tccagagtat taaataacca gccaaatatc tctgataaag
    18781 caaaagcaag tgtacttcaa gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa
    18841 gaggactgtc aggaaatggt tttgacgcga ttctagcatt atctcatcgt acttctcata
    18901 cgacctatgg caatcctttt ttttcagatg ttattcaatc catttctgcc gtttctgaac
    18961 aaaataactt cgacttgatt ctacaaacgg caaaaacacc agaagaagag ctagaaaaat
    19021 gcgcgaaaaa gatccaagaa aaaatgatcc gaggaattat tattttaagc tctacgatgg
    19081 atgaaacact tctttttgaa ttggatcaat acaatattcc tattgtggta atcggaaaag
    19141 tcggaaacga ctacacgaac atctactcag tggacacgga taatttccag gatagttacg
    19201 atttggtgga aacactgatc aagaatggac accgtcatat cggctgtctc catgctccga
    19261 cagaagttta tgtatccaat gatcgtctca atggttaccg tcaatgctta tttgatcacc
    19321 acctagatat ccgtaatgaa tggattatag acggcggctt tcgcttagaa gatagcatgg
    19381 cagcaatgaa tgatttaatg aagctacgag aaagaccaac tgcgattttt tctaccgatg
    19441 cattaaaatc attgagtatt tacaaagtgg cagaacaatc tggaaagcag atacctgatg
    19501 atctttcgat cgcaggattc agtgatccct tattgtcctc cctattttcg ccacagttga
    19561 ctcggataga cattccgaca aatcagttag gaatcaaagc cacagaactt ttattccaac
    19621 taatccacca ccaataccct gaagaaaaaa gaatcattct ttcaaccgag attactctca
    19681 cagattcgat ccaacaacaa gaaatgtaga acagaattat aaaagaacag aaacactgag
    19741 gataagtcag ttactctgtt cttttttcaa tcactcatcc ctcgttaccc cctcccggta
    19801 ttttagcgaa tttttcttat tttccatctg ttttagatag ctgtggataa ctataatgtt
    19861 tgtgttaaac tattttttcc acagggaaga aaaaactatt tttaaccacg ttgtgtataa
    19921 gtttttatag ttcgtttttt taaacacatt tacccttatt tcaatccatg ttatttgggg
    19981 atacttatct ctaaaacata aatagaataa cagacatgtc tactggtttt gttttattcg
    20041 aggaattatt caataatagc tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa
    20101 tttattttgc attcaaaaaa tgatctatct ttacttttgt tgattgactt ctcttggaag
    20161 atgatcgttc cgatacctta attggatacg aaccgatgtt gttaagatcc tttcccactt
    20221 ttcttctagt tagacaaatt attttagaca aaccaaaaaa gaggttgtga caataatttg
    20281 tcacaacctc ttttttaaaa tatctatttt gtttgattgt tcattgcgtc ttctgcagcc
    20341 atttgcgctt caatatctga aatactgtag acattttcgc ttgctacacc gacttctttt
    20401 ggttccatgt cacgataacg ttgcatacca gtaccagctg ggatgatctt acctataata
    20461 acattttctt tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc atccgtcaag
    20521 acacgagttg tttcttggaa tgatgcagca gataagaaac tatttgtttc taatgatgct
    20581 tttgtgatac ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga gactagcgta
    20641 ttgtagtttt gatctttaaa gtctgcgatg tctaataatg tacctggtaa gatatctgta
    20701 tcacctggat ccattacacg aactttacgc agcatttgac gaaccattac ttcgatatgc
    20761 ttgtcgccga tttctacccc ttgcatacgg taaacacgtt gtacttcacg aagcaagtag
    20821 ttttcaacag ataatacatc gcgaacttgc aacaattgtt ttggatcgat tgatccttct
    20881 gtcaatggtg caccacggtg gatcatatcg ccttctgcta ctttcatacg tgctgtataa
    20941 ggaacagtat acgtacgtgt atctgttttt cctttgattg ttacttcttt ggtacgtgtt
    21001 gcaggatctt ctgagatatc gatgacatcc cctgttactt ctgtgatcac tgcttgccct
    21061 ttcggattac gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt gatatcgtct
    21121 ccggcaaccc cacctgtatg gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt
    21181 gattgagcgg cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc agttgcaagg
    21241 ttgcgtccgt aacaatgttt gcagacacca tgttttgtat tacatgtgaa gacagaacgg
    21301 attgtcactg tttcgacacc tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat
    21361 tggtttcctg caatgatcaa ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa
    21421 cgtcccacta aacgatcttc taatgattcg atgatttcat ttccctcgcg gatggcttgg
    21481 atttcaagac ctcggtcagt accgcaatct tcttcacgga tgattacatc ttgggccacg
    21541 tcaaccagac gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc
    21601 cctttacgag ccccgtgagt tgagataaac atttccaaga cggatagtcc ttcacggaag
    21661 ttcgaaatga ttggcaattc catgattcga ccattaggag cggccatcaa accacgcatt
    21721 ccggcaagct gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga catcatgaag
    21781 attggatttt tcgcttccaa accttccatc agtttttgtt ggatgctgtc ttttgcagca
    21841 ttccaaacag caatgacacg ttcataacgt tcgtcatctg tgatcagacc acgacggaat
    21901 tgttttgtaa tagtttcaac ttgtttatgg gcattttcaa tgatttcttg tttttcattc
    21961 agtacactga tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg tttgtaccct
    22021 aagtctttca tacggtcaag catcttagaa gtttctgtta ctttgaatcg tttgaagact
    22081 tctgcgatga tgtttcctaa gtttttcttc ttgaacggta atactaattc ttgctctttg
    22141 atatgtgcag gaatatcagt accagcttca acaaagtatt tgtccggtgt ttggacggtc
    22201 aagttgaagt ctgttggttc attcagataa gggaattcag gaggcatgat ctcgttgaag
    22261 atgatcttac caactgttgt gatcatcgta cgttcttttt gccattcagt aaatggtttt
    22321 tcacctaatg agttagggtt cacaccgata cgtgaatgta aatgaacata tccgttgcgc
    22381 catgcgataa cggcttcgtt ttgatctctg aagaccattc cttcgccttc gcgtccttct
    22441 tcttccattg tcaagtagta gttacctaga accatatctt gggaaggagt aacaactggt
    22501 ttaccatctt tagggttcaa gatgttttga gcagctaaca tcaacatacg tgcttctgct
    22561 tgtgcttctt cattcaatgg aacgtggaca gccatttggt ctccatcgaa atcggcatta
    22621 taagcttcac atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa aactggttcg
    22681 aatgcttgga taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc
    22741 ttgattacgt cttctaaaac atcccatact tcgtcttctt gacgttcgat tttacgtttc
    22801 gcatttttga tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac gaatggtttg
    22861 aataattcaa tggccatttc ttttggaagt ccacattggt acatcttcaa gaaaggtcca
    22921 acaacgataa cggaacgacc tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa
    22981 cgaccttgtt tccctttcaa catatgagaa agagatttca atgggcggtt acctggtcca
    23041 gtaactggac gtccgcgacg accgttatcg atcaatgcat ctactgcttc ttgcagcata
    23101 cgtttttcat tttgaacgat gattccaggt gcattcaagt ctaacaaacg tttcaaacgg
    23161 ttattacggt tgattacacg acggtaaaga tcattcaagt cacttgttgc aaaacgtcca
    23221 ccttctaatt gaaccattgg gcgtaaatct ggtggaataa cagggataac gtccataacc
    23281 atccagcttg gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa acggcggatt
    23341 gcacgtgtac gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc ggctgcttct
    23401 gcatctagat caacattatc aagtaattgt ttaatcgctt ctgcacccat agcagcttgg
    23461 aattcttgtc catattgttc acgtttttcg cgatattcgc gttctgtcaa caattgtttt
    23521 ttctctaaat tcgtatttcc tggttcgatg actacatatg aagcaaaata aatgatttct
    23581 tccaaagcgc gtgggctcat gtctaataca agacccatac gtgaaggaat ccctttaaag
    23641 taccagatat gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc acggcgaact
    23701 ttcgaacgag taacttccac tccgcatcga tcacaaacga ttcctttata acggatacgt
    23761 ttgtattttc cgcaggcaca ttcccagtct tttgtaggac caaaaatccg ctcacagaac
    23821 aacccttcgc gttcaggttt taaggtacga tagttaatcg tctcaggttt ttttacttca
    23881 ccgtaagacc agcttctgat tttttcggga gaagccaaac ctatttgcat actttcgaat
    23941 ttatttacat cgatcaaaag gggttccctc catttcattt aatggcaaac cgcaagacgg
    24001 ctgatcttcg accgccttgc agctcataga ccggattagt ctaaattatc ttctgcggtt
    24061 tcaaagtttt ggatgacatc gtctttttca tcttccactt gttcagcagc tttcttttct
    24121 aattctttgg cagtttgctg ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg
    24181 tcgtcttcat cgtccatgtc gcgaagttcg atctctgttt cttcgatatc caagacacgc
    24241 atgtccagac ctagtgattg caattctttc accaatacgc ggaatgattc cggtacacct
    24301 ggttttggaa ttggttcacc cttgacgatt gcttcgtatg ttttcacacg tccgactacg
    24361 tcatctgatt tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta agcttccagt
    24421 gcccatactt ccatttcacc aaaacgttgt ccaccaaatt gagctttacc tcccaatggt
    24481 tgttgtgtaa ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg
    24541 tgagcaagtt tgatcatgta catcacaccg acagacacac gtccatcaaa tggttcacct
    24601 gtacgtccgt cgtacagaac agttttcgca tcgctggcca taccggcttc acgaactgtt
    24661 tcccatacgt cttcatcact tgcaccatca aatactggcg ttgctacgtg gatacctaac
    24721 tgacgtgcag ccatacccaa gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc
    24781 acccctaaag gattcagcat gatatcgatt ggtgttccat caggtaggaa tggcatatct
    24841 tcttccggca taatacggga tacaacccct ttatttccgt gacgaccggc cattttatcc
    24901 ccttcatgga ttttacgttt ttgaacgata taaacacgaa ctaacatgtt tacgcctggt
    24961 gacaattcat ctccagcttc acgagtaaag attttcacat cgtgaacgat accgccgccg
    25021 ccatgtggta cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca
    25081 tgcaatagac gttcttctgc agataattct gttacccctt ttggcgtgac tttccctaca
    25141 agcaaatcgc catcttggac ttctgcacca atacggatga tacccatttc gtctaaatct
    25201 tttaatgcgt cttccccgac attagggatt tcgcgtgtga tttcttcagg tcctaatttt
    25261 gtatcgcggg cttctgattc atattcttca atatggatag aagtgtatac gtcatctttt
    25321 accaaacgac ggctcatgat gatcgcatct tcatagttgt acccttccca agtcatgaaa
    25381 ccaactaaaa cgttttggcc tagagccatt tcgccttgtt ccatagatgg tccgtctgcc
    25441 agagtatccc ctttttcgac tttctcaccc aaatgaacga ttggacgttg gttgtagctt
    25501 gttccggagt ttgaacgacg gaattttgtt acatcgtatt tatctaatgc accgttgtcg
    25561 cggcgtacgc ggatttcaga agcatcaacg tattcaacga caccgtcatg tttacataac
    25621 aaagcagctc ctgagtcatg cgctgatttg tattccatac cagtaccaac ccatggtgct
    25681 tgaggattga tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg
    25741 gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac ttgtttaggt
    25801 gatacgtcca tgtagtctac tttatcaata gatacttcta agttttcact ttgagcacga
    25861 gccataacaa cgtcttgagc aaatgttcca tcttcattca atgggctgtt tgcttgggct
    25921 acgatgtaat ggtcttcgat atcagctgtc aagtagtcga tttggtcagt aacacgacct
    25981 gtttcacggt ctacacgacg ataaggtgtt tcgataaatc cgaatttatt tactttcgca
    26041 taactggaca aactattgat caacccgatg tttgggcctt caggagtttc gatcggacac
    26101 atacggccat agtgggagta gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc
    26161 aatccgccag gtcctaaggc tgaaagacga cgtttgtgtg tcaattcgcc aagagggttt
    26221 gtttgatcca tgaactgtga caactgagaa gaaccaaaga attctttgat acttgccaca
    26281 actggacgga tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt
    26341 ctttcacgaa ccacacgttc catacgtgct agcccgatac ggaattggtt ttgtaaaagt
    26401 tcaccgactg aacggatacg acgatttcct aagtggtcga tatcatcgac attgccgata
    26461 ccttccatca agttcaagaa atagctcatt gaagcaatga tgtcggctgg acgaaccgtt
    26521 tttacagctg cttctggata gccgtttccg atcacgttta cttcacgttc agggtctctt
    26581 ggtgaaaata ctttgatgac ttggacagtc attggatctg tgaccacacc gtcttctgat
    26641 ggatagtaag tcactgaatt taggccatta tcaatgaaag gagctagtgt ttccatgact
    26701 tggtgagtca gaactgttcc tttttcaacg atgatctcgc ctgtttctgg atcaactaat
    26761 gtttcagcta atgtcagatt caatagacgt gttttcaaat ctaatttttt gtttactttg
    26821 taacgaccaa cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc attcagcaag
    26881 ttacgtgagc tgtctgctgt ttttggttcg cctggacgca aacgttcgta aacgtctttc
    26941 aaaccttctt ctgttcttga gtcgcttgcg tttttatgca agtctttttc gactgtattg
    27001 cgtaatgttt cgctgtcgcc gaagatttca aagatcgtat catcggaacc aaatcctaac
    27061 gcacgaacta atacggttaa aggaatctta cgtgtacggt cgatacgtac ataagagatg
    27121 tcttttgcat cagtttccat ttctagccaa gctccacggt tagggataac agtagaaccg
    27181 aatccttctt ttccgttttt gtcgactttt ccatggaaat agacacctgg tgaacggact
    27241 aactgagaaa cgattactcg ctctgccccg ttgatgatga atgttccttg ttctgtcatt
    27301 aatgggaaat caccgaagaa tacttcttgt gctttgattt cgccagtctc gcggtttgtc
    27361 aaacgcaatg ttacatgtaa tggtgcagag tagtttgcat catgcgcacg agcttctgct
    27421 actgtatact ttggttcttt caattcataa tctacaaatt ccaaagatag attgccattg
    27481 aagtcatcga ttggcaaaat gtcttcaaac atttctctaa gtccctcatc taagaaccat
    27541 tggtaagagt ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc actgattctt
    27601 gcgaaacttc tacgttcgcg atgttttccg tattttacta cgtgtccagc caagctcttc
    27661 acccctctaa aatgtcattc aaaaagacta cccgatcaat tgttacaata atatttcaaa
    27721 tattattttt aggtaacaac agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa
    27781 tagaacattc tcttgaaacc ttacttcgtg agcttctact tttgtttcct tatattaaag
    27841 ccgaaacgga caatatttcg tttctgcctt ttcttcatca gatagttttt gcaacttcat
    27901 atactacact tattttctat aaaagtcaac acttcctggc gtttcttctg ctttttcgca
    27961 acattttctt aatgttttca attttatgtt ttcaattatc tgacaattct taattgacag
    28021 acctttttat ctcggttata ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac
    28081 caacatcaac caaatcgcac caacacaact gaataacttt tactttagct actttagata
    28141 ggtttgtatt catcatgatg gatacaaacg ttaaacgaca gtttgtatct atgatggcta
    28201 aagaactttg tattgcagca aaaagccacg tagacaaaat gctaagtggc tgagcagtga
    28261 aaatacaaag taaaagtatc aagcattcta ctttttcaat ttattttccg acttttcaat
    28321 ccatttggac attttgtctg aatggatttt ttgtttccca aaattaagga ggaaaaagaa
    28381 atgaaaaaaa atataatcat cggattaggc gctgccctgc tactagcttt aggaggttgc
    28441 gcacaaaaag acaattccgc agaggcatca gatggacaaa aaacaattgg cgtattgcaa
    28501 cttgtacaac ataactcgtt agatgctgcc tataaaggat tcaaagaagg attagcagaa
    28561 gaagggtata aagaaggcga taatctaaca atcgattatc aaaatgccca aaacaatcaa
    28621 gacaacctga aaagtatgag tgagaaactc gtcaaagaag atcctgattt gctacttgga
    28681 atcgccactc cagcagcaca atctttatta aatgaaacaa ccactatccc gatgactgtc
    28741 acagcggtca cagacttgga acaagcccat ttagtcacat ccaatgaaaa acctggcggc
    28801 aatgtgactg gcacgacaga tatggtgcca atcgaaaaac aaatcggctt actgttaaat
    28861 atcgtgccag acgccaaaac aatcggcatc atgtataaca acggtgaagc aaactctaag
    28921 atccaagctg atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg
    28981 acagcaaatt ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat
    29041 ggaatctata ttcctactga taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc
    29101 gcaaaagaaa caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt
    29161 ctagcaacag taggaatcga ctacgaaagc ttgggtaaac aaacagggat catggcagca
    29221 aaaatcttag acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa
    29281 ctgtatgtca ataaagacat ggccaaagca ttaggcatca atccagacag catcaaagaa
    29341 gctgaataaa acacaggagg attctcatga caaatttgct ctctcttttc ttatcatctg
    29401 catcgcaagg ggtcttatgg gccctacttg cgattggtgt atacgtcact ttccgtatct
    29461 tggacattgc cgacttgact gctgaaggaa gctttccttt aggtgcaggg atcgcggcag
    29521 taagtattac aaatggctat catccattga cagcttgtct tctaggcttt ttagggggtg
    29581 cggcagcagg gcttgtttcc ggactgcttc acacaaaatt gaaaattccc gcccttcttg
    29641 cagggatcat caccatgact ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga
    29701 acatctcttt attgggtcag ccaacgatct ttcattgggt cgagtcattc ggcataacaa
    29761 aagacaacgc tgtactgatc gtcggtctgc tttttgcttt gatcgttgta tgtttacttg
    29821 ttttgttctt acgtacagaa acaggacttg ctattcgtgc aactggcgat aatctggcaa
    29881 tgagcgaagc aaacgggatc aacacagata atatgaagat catcggttat atgatttcta
    29941 atggatgcat tgctctttct ggctccttgc ttgcgcaaaa caacggcttt gctgatttaa
    30001 attctggtat tggaacgatc gtaattggac ttgcttctat tatcatcgca gaagttttgt
    30061 tacgtaacca gccactattt ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc
    30121 gttttatttt agcgttagtc ttcgaactga atgttgaacc aagtgattcg aaacttgctt
    30181 ctgctttcgt gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa ttccatttac
    30241 ctcaaaaaaa gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca
    30301 tcataccttt gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc
    30361 cttagatccc ggcgacttcg tgacgatcat cggtggaaat ggggctggta aatctacttt
    30421 gctgaacagc atcgccggta cattttctgt cgatcagggt caaattttat tgaatggaaa
    30481 aaatatcacg aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga
    30541 tcctaaacta gggacagccg ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa
    30601 acgaggaaaa aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa catttttcaa
    30661 agaccagctg gttcggctaa atttggggtt ggaaaatcgt ttatcctcag aaatcggttt
    30721 gctgtctgga ggacaacgtc aagctattac gttattgatg gccacattac agcgtccaga
    30781 actgatctta ttagatgaac acactgcagc gcttgaccct gctacttcca aaacggtgat
    30841 ggaattgaca gaaaagctga tcactgagca gaatctcact gcttttatgg tcactcatga
    30901 catggaagac gcgatccgct atgggagtcg actgatcatg ctgcatcaag gaaggatcgt
    30961 cgtagatatc cagcaagagg aaaaacagca gctcacagtg ccccaattat tagcgttgtt
    31021 caaacaaaac agcggcacgg cattaaaaga cgatcaagta cttttatctt cataatcaga
    31081 aagaggatgg gacaaaaaat gaacgcccac cctcttcttt tttctctttc cttctttcgt
    31141 ttttgcttta tgtatcaacc gttgtttctt cgaaaactat tttgttctct ccttcttcct
    31201 gatagaatgg atgggaaagg agcttgatgc catgacaacg aaagacaagg tacttcaatt
    31261 attgaaaaag acaccagatt ttctttccgg agaaaagttg gcacagcaac tggaagtttc
    31321 ccggacaagt atttggaaag ccatcaaaga attggaaaag gcaggctatc gttttgagca
    31381 cgggcctaca gggtatcgtt acctgccctc tgacgtacta gatgcaaaag aaatccagca
    31441 agagctacgg tcgtttattc ctgatttgaa agtacaaacg atgttgactt ctgaatctac
    31501 gatgaaagat gtcaaactgg cagcagctca aggagattct gtttcgaagt tatttgttgc
    31561 tgatacacaa gaacaagctc gtggacgttt tggacggcct tttttcgctg aacctggacg
    31621 aggaatctac atgagtctct tgctccatcc agacaaacgg tttgatgaat tgcctcaata
    31681 tacgatcatc gcagccagtg cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc
    31741 ttcgatcaaa tgggtcaatg atatctacat ggatggaaaa aaagtctgtg ggattttatc
    31801 tgaagcagtc agtgatatgg aaagcggcag gatcagtcat atcatcattg ggatcgggct
    31861 taatttttct gttcctcaaa atcattttcc agctgatctc caagaaaaag ccatctctgt
    31921 ctttcccgac ggacaagcta gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga
    31981 attgttcact tatctttctc acccagaaaa agcgttggac ttctataaag aaaaatcatt
    32041 cgtattggga aaaaccgtgt cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa
    32101 agaaatcggt gatcatggcg aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc
    32161 ttctggcgaa atcagtctca catctatccg tacgcaataa ttatcagatt tcacaaaagg
    32221 acacctgatc tcatacagtg tccttttttc tgaattctct tcaaaagact ctccttattc
    32281 tatcagctga cagtttctct atcctctaga aaaaggtatt ctaaaaatat ctataaagca
    32341 tggtacactt tctttaaatg aatcaatgca aaaaacaaga gagtcatcag tttacacgat
    32401 caaaagagat agcaggccgc ttcctttttc aatcttatag atagaccatg ggtaacgata
    32461 aaaacaaaaa agaacttgcg acttattttg ccgtaagttc ttttttagct agtgccttaa
    32521 tctcttaatc ccaacaaacg tctcgcatag atcacctgac cagagtgcat agcagcatca
    32581 tcaataatag aaacgagacg cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt
    32641 acttcatcta gctcggaagg ttccagcctt cttaagtaag aaatagcaaa atcagcagct
    32701 cctcgcaagt atcctagcag atcttctatt tgattagctt ggatctgctt tgcttgttcc
    32761 agactatgct tccaatcttg ttcgttatct gctatcgtca tcggaaaccg gctttcccag
    32821 tcttgactat gccaaattgg agaagttcct gctaaatcag caatctggaa atctaattct
    32881 cgagcggtat gccaagccag ccaagtcatt gatttgatct gcggggctct atcttccact
    32941 ggaaaacgat tggcctcctc aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat
    33001 gcccgatcca cactctcgca tagcatatct aaataatcca tttattttcc tcctttttat
    33061 ttatcctact tactttttcc taattctagg atacctactt atatgttact acacaccctt
    33121 attttttaca ctttaacagt caccatcatc aatagtttgg agattgaaag aaaaagatag
    33181 tgtcgaacta tcaattgaac aagaagcaac aatatgaacg tttcattaat attctttaaa
    33241 taaacaatga tttttcaaac gaacagttta agactaggag aaaaagagta taataggaaa
    33301 atgtttgaaa ggaaaggaag gggaaaaatg aaaaaaaggg caagtttact agtcctagtt
    33361 gctttgttgt tcaatatctt ttcgtatggt gtggccgttt cagcggaaac atacattcct
    33421 aaagaccctg ggaatattta taatgatcta actggtaact atggcttgtt aggaattgct
    33481 agtcaattcc acgtatttgc taaaaataag acaactgtga atgcacatac agacggaaat
    33541 atagcaacac atgaacttga tgcaaaaaat aattttggta cagagcttca tgaaggttta
    33601 ttgaaccaag aaataaatta tatacaaaaa actgattctt tcgctaattc ttcaggcata
    33661 cctacaggta gcacaagaat gaataaattt gtcgttggca gtaacgtgga tgtagcactt
    33721 caagatggtc atcctacagt caatggatcc aggcttgatc acttaactcc agatgatttt
    33781 tataaagatc gtgctgggca tgaatatatt gatttcgagc aggaatttgc taaattgaat
    33841 gtagcagcaa atgatcttgc aacgatcact ccagctaaaa catatactgc tgcagatttt
    33901 ccagacatga acaatcggac aatcgattta actgatttat cagataccgg gtttttattg
    33961 gtaaatatcg atgcagaagt gttaacgatg aatacgccac ttcaaatcat taatccaaat
    34021 gatcaagtag ttgtatttaa tgtcatcaac agttcttctg cattgaacgt acagtcacca
    34081 atcaaataca atgatcgttc aaatcatgaa acagaagatt tttcggatgc gaatatctct
    34141 tggaattttg gaaatgagat gactgatttg acaatcagtg ctccttttca aggaacaatc
    34201 cttgcgccta atgctactat ccgcattact caaaaccaag acggaacgat tataggaaca
    34261 aatgtgattt tagatgcagc taccaatcgt tgggatccta acgaaatatt tatcagcgac
    34321 aatggtacag atacgacaga cacaagcgac agtacagata catctaccac aagtgatagc
    34381 tcagattcat caacttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    34441 gatacatcag cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggac
    34501 acatcagctt caagtgacag tacagatact acctctacca caagtgacag cacggacacg
    34561 tcagcttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac ggacacatca
    34621 gcttcaagtg acagtacaga tactacctct accacaagtg atagcacgga cacgtcagct
    34681 tcaagcgaca gtacggatac atctaccaca acaagtgata gcacggacac gtcagcttca
    34741 agtgacagta cagatactac ctctaccaca agtgatagct cagattcatc taccactaca
    34801 agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc taccacaagt
    34861 gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac cacaagcgat
    34921 acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac gaactttaac
    34981 ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact tcctaaaact
    35041 ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt aatcggttta
    35101 agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca agataaaaaa
    35161 gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa atacaagaac
    35221 tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat ttacgtatcg
    35281 aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa aagagattct
    35341 cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt
    35401 aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg attttgcgta
    35461 cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt agtagctttg
    35521 gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga cgcatgtctt
    35581 ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata tcatcgataa
    35641 tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg aagcgctcgc
    35701 ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca gctgtcttac
    35761 ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga tcatccatct
    35821 gccctaattc gccattcaat actttttcca tactgcgaga attcaattgg agacttttca
    35881 aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag tctcctgcat
    35941 agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt cgcagatccg
    36001 cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg atagaagctg
    36061 ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac aatagctgat
    36121 aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgattgct tgttcgacag
    36181 gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc aattctttac
    36241 ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc cttgtgtgac
    36301 gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc ataatctttt
    36361 tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat cccgattgca
    36421 atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac tgtccatgat
    36481 tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc gccggccgca
    36541 ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa cattccaaaa
    36601 taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc gcctagcaaa
    36661 gtagtcagga atagtcgtag ccaaagtagt aagaagctgt ctcgtttttt catggtaaaa
    36721 atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc aaaagaaaaa
    36781 ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac aagtagcgaa
    36841 atataaatca ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa tcagactgtc
    36901 ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca aacagacgat
    36961 ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat cgcaatctgc
    37021 ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc cgtgttcatc
    37081 tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt tgatttctgt
    37141 gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct gaagaccaaa
    37201 aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa catcccaagg
    37261 tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc tttttccaga
    37321 ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag
    37381 taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg ggcttattac
    37441 cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag tgggcaactt
    37501 caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa tttcggctta
    37561 tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa cagaggttgt
    37621 gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt aattttacat
    37681 aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg cattcaaata
    37741 acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt accaccaatt
    37801 acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg cacgtccgga
    37861 accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt aaaacatgcc
    37921 tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa
    37981 tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt tttggaagaa
    38041 cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc agtctggtga
    38101 cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc
    38161 ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc
    38221 agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc aagggaacac
    38281 actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga acacttcacc
    38341 attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga aaatacgaag
    38401 tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc
    38461 atctttgata tggaaaaact catgcattaa atattggaac atataataac cagaacgaat
    38521 atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa atccgcttaa
    38581 atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa ccataaatcc
    38641 ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca cgatattcgc
    38701 tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg
    38761 gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt cgtagccttt
    38821 tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc
    38881 agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt
    38941 gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg cattcaataa
    39001 taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa
    39061 catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt ttggatctaa
    39121 tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca tttcaatacc
    39181 ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt catcatctgg
    39241 ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta cagaatcttc
    39301 aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc
    39361 tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg tcttgacgat
    39421 ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat tcttttgacg
    39481 agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc cgccagctac
    39541 ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag taattgctac
    39601 atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg catatcctgc
    39661 tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag actatctata
    39721 taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc atatattata
    39781 tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt aaaataaagc
    39841 gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt tatttatcaa
    39901 aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa agaaaaattg
    39961 tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg taactggatt
    40021 tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag
    40081 ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa gcacaacaga
    40141 atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata aattagaaga
    40201 aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat
    40261 cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga
    40321 atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca ttcctgagtt
    40381 gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac
    40441 tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg ctcaagctgg
    40501 taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca cttacaagct
    40561 tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag ttgctggcgg
    40621 caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa aacaagatga
    40681 tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc
    40741 tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt
    40801 agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg cacaacaatt
    40861 agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata tcgtaacaga
    40921 aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc cagattaggc
    40981 agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact actattttcc
    41041 ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc agcaaataaa
    41101 acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc tgatgaacag
    41161 ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga atcagcattg
    41221 aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt
    41281 tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt ttctgatgac
    41341 gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg cggattcgat
    41401 atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc cagaaaacct
    41461 tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat cgaactaaat
    41521 gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc
    41581 gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg agtgaccact
    41641 gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag aggcgaaaac
    41701 caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt tttaggttct
    41761 attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta tttagaagta
    41821 gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat
    41881 atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt gtctacagca
    41941 gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt gaataacggg
    42001 acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc aatcaaagac
    42061 accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga actaaaataa
    42121 ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg
    42181 gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt tcatgagata
    42241 cattggctaa acactttttt attttttatt ggaatgctag tttttgattt agcgactacc
    42301 gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa
    42361 aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg
    42421 attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt
    42481 ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc gattccttta
    42541 tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc
    42601 atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca
    42661 tctggacaat ttgctttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc
    42721 ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac
    42781 cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca
    42841 gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc
    42901 ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat
    42961 ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat
    43021 atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc
    43081 atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca
    43141 aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt
    43201 ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa
    43261 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc
    43321 ttttgaacac cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa
    43381 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg
    43441 cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa
    43501 acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc
    43561 tgattgaaca ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc
    43621 ccttttttca cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt
    43681 aactatatgg tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc
    43741 acactttttt aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa
    43801 tattttgaac ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga
    43861 taatcaaaat ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac
    43921 ataaataact aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg
    43981 gtttgttcaa taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt
    44041 cttccgctcc tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca
    44101 tcttgatagc cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta
    44161 agccgacgat tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat
    44221 aatcatttaa tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt
    44281 cgccatattt ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg
    44341 taaagacgac tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata
    44401 aacgttctga taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga
    44461 tcacatcacc aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga
    44521 taaacccttt tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg
    44581 gtacacgtcc accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata
    44641 gcaccgtata ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt
    44701 ttattccagc ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc
    44761 taagtggtcg ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac
    44821 ctgaaagttc agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg
    44881 ttaattcaaa aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac
    44941 ttgcacggcc tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt
    45001 cgatcgtttg actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat
    45061 ctaatcctct ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta
    45121 gaccggcgaa atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt
    45181 caattgtttc caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt
    45241 tgacacatgt tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc
    45301 ctgctctgtt tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata
    45361 tttgcatttt gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt
    45421 ttctacagaa aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca
    45481 gggatatatc agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc
    45541 tgtgcgactg gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct
    45601 tcttcattat gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat
    45661 tcgatttcat ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc
    45721 tcatcataag ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg
    45781 aataattgtt cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt
    45841 gctactttcc ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc
    45901 atataactgt ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat
    45961 ttcattcttc cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt
    46021 tcgatcattg acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca
    46081 gttaaactgt ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa
    46141 gattgagaac atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat
    46201 cgaccagaat aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta
    46261 tttttacgaa tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc
    46321 aacccttctt ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat
    46381 aaatggatcg gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa
    46441 acaatcactg cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct
    46501 tcatttcctt catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt
    46561 gcaaaagcaa ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa
    46621 ccatatgcat tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac
    46681 ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc
    46741 atttctttca tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat
    46801 ctaattcacg gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt
    46861 cttgatccca gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt
    46921 ttacgaattt ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct
    46981 gataaagttc attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc
    47041 catgactatc ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa
    47101 gacctctttc acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca
    47161 gtggacgttt ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat
    47221 cgagtttttc ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc
    47281 caactgcgcc ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag
    47341 catcataaat atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg
    47401 tatcaccaac tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta
    47461 catttttcat tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat
    47521 acttgtgtgc taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac
    47581 gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt
    47641 ctgcttgttc aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca
    47701 acttcataat agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga
    47761 tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata
    47821 aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa
    47881 attcagctac acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga
    47941 acattcaatg tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt
    48001 tctccgccaa gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg
    48061 attggacgtg caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat
    48121 ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg
    48181 atttggacga ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg
    48241 attcgattca agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc
    48301 atggctttgt gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga
    48361 atttgtgtcg ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt
    48421 ctacctttag aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg
    48481 tctggatgtg ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt
    48541 ttttctgtca actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat
    48601 cggcttgtac tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca
    48661 cttccaaaca tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt
    48721 ccaaggaaca tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg
    48781 cggatcgcac gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga
    48841 agtccttcca gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca
    48901 ccagtccact ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct
    48961 tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct
    49021 tgtctgcgta attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct
    49081 tgtactagtt catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga
    49141 tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga
    49201 cgagaagtga tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc
    49261 tccactccat ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct
    49321 tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt
    49381 tcgatatatt tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga
    49441 gctaacgccg gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca
    49501 attaaggtat gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg
    49561 atgacctttt tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat
    49621 cccatatcaa gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg
    49681 attttttgag ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt
    49741 acagggttat tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa
    49801 cgaccaacaa ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat
    49861 gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta
    49921 cgacgattca ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact
    49981 tgacgggctt ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt
    50041 tcatctttta ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc
    50101 ttcacttcgc tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag
    50161 actcctttag ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa
    50221 atttccgttt cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg
    50281 accactaagg ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct
    50341 tcttcttgtg caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa
    50401 tctcacttcc catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt
    50461 gtatgatctt catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag
    50521 tttccttctt tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca
    50581 tttttctcac tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta
    50641 tctgaaatcc ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact
    50701 agataaccac gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca
    50761 cagttaaaga ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga
    50821 attttcttta tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt
    50881 ccctttgact aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg
    50941 ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattc
//
LOCUS       DASJTM010000007        49954 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJTM010000007 DASJTM010000000
VERSION     DASJTM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.007"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..94)
                     /locus_tag="U4B29_000373"
     CDS             complement(<1..94)
                     /locus_tag="U4B29_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2105345.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVL"
     gene            complement(96..293)
                     /locus_tag="U4B29_000374"
     CDS             complement(96..293)
                     /locus_tag="U4B29_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN2105346.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(687..2414)
                     /locus_tag="U4B29_000375"
     CDS             complement(687..2414)
                     /locus_tag="U4B29_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN2105347.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2407..3600)
                     /locus_tag="U4B29_000376"
     CDS             complement(2407..3600)
                     /locus_tag="U4B29_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2105348.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3787..4431)
                     /locus_tag="U4B29_000377"
     CDS             complement(3787..4431)
                     /locus_tag="U4B29_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2105349.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4525..4659)
                     /locus_tag="U4B29_000378"
     CDS             complement(4525..4659)
                     /locus_tag="U4B29_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN2105350.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4661..6793)
                     /gene="feoB"
                     /locus_tag="U4B29_000379"
     CDS             complement(4661..6793)
                     /gene="feoB"
                     /locus_tag="U4B29_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN2105351.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6790..7263)
                     /locus_tag="U4B29_000380"
     CDS             complement(6790..7263)
                     /locus_tag="U4B29_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN2105352.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7664..7888
                     /gene="nrdH"
                     /locus_tag="U4B29_000381"
     CDS             7664..7888
                     /gene="nrdH"
                     /locus_tag="U4B29_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN2105353.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8047..10206
                     /gene="nrdE"
                     /locus_tag="U4B29_000382"
     CDS             8047..10206
                     /gene="nrdE"
                     /locus_tag="U4B29_000382"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN2105354.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10256..11221
                     /gene="nrdF"
                     /locus_tag="U4B29_000383"
     CDS             10256..11221
                     /gene="nrdF"
                     /locus_tag="U4B29_000383"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN2105355.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11310..12449)
                     /locus_tag="U4B29_000384"
     CDS             complement(11310..12449)
                     /locus_tag="U4B29_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2105356.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12758..13471)
                     /locus_tag="U4B29_000385"
     CDS             complement(12758..13471)
                     /locus_tag="U4B29_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN2105357.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13725..14426)
                     /gene="nagB"
                     /locus_tag="U4B29_000386"
     CDS             complement(13725..14426)
                     /gene="nagB"
                     /locus_tag="U4B29_000386"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN2105358.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14660..16192
                     /locus_tag="U4B29_000387"
     CDS             14660..16192
                     /locus_tag="U4B29_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2105359.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16383..17585)
                     /locus_tag="U4B29_000388"
     CDS             complement(16383..17585)
                     /locus_tag="U4B29_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2105360.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17667..18356)
                     /locus_tag="U4B29_000389"
     CDS             complement(17667..18356)
                     /locus_tag="U4B29_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2105361.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18462..18830)
                     /gene="rplL"
                     /locus_tag="U4B29_000390"
     CDS             complement(18462..18830)
                     /gene="rplL"
                     /locus_tag="U4B29_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN2105362.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18883..19386)
                     /locus_tag="U4B29_000391"
     CDS             complement(18883..19386)
                     /locus_tag="U4B29_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN2105363.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19590..19717)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19796..20485)
                     /gene="rplA"
                     /locus_tag="U4B29_000392"
     CDS             complement(19796..20485)
                     /gene="rplA"
                     /locus_tag="U4B29_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN2105364.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20589..21011)
                     /gene="rplK"
                     /locus_tag="U4B29_000393"
     CDS             complement(20589..21011)
                     /gene="rplK"
                     /locus_tag="U4B29_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN2105365.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21354..22022
                     /gene="sdaAB"
                     /locus_tag="U4B29_000394"
     CDS             21354..22022
                     /gene="sdaAB"
                     /locus_tag="U4B29_000394"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN2105366.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22035..22907
                     /gene="sdaAA"
                     /locus_tag="U4B29_000395"
     CDS             22035..22907
                     /gene="sdaAA"
                     /locus_tag="U4B29_000395"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN2105367.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22998..24017)
                     /locus_tag="U4B29_000396"
     CDS             complement(22998..24017)
                     /locus_tag="U4B29_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2105368.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(24190..25095)
                     /locus_tag="U4B29_000397"
     CDS             complement(24190..25095)
                     /locus_tag="U4B29_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2105369.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25154..25699)
                     /gene="nusG"
                     /locus_tag="U4B29_000398"
     CDS             complement(25154..25699)
                     /gene="nusG"
                     /locus_tag="U4B29_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN2105370.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25800..25970)
                     /gene="secE"
                     /locus_tag="U4B29_000399"
     CDS             complement(25800..25970)
                     /gene="secE"
                     /locus_tag="U4B29_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN2105371.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25994..26146)
                     /gene="rpmG"
                     /locus_tag="U4B29_000400"
     CDS             complement(25994..26146)
                     /gene="rpmG"
                     /locus_tag="U4B29_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2105372.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26308..26952
                     /locus_tag="U4B29_000401"
     CDS             26308..26952
                     /locus_tag="U4B29_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2105373.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27008..27511)
                     /locus_tag="U4B29_000402"
     CDS             complement(27008..27511)
                     /locus_tag="U4B29_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2105374.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27524..27570)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28108..29073
                     /locus_tag="U4B29_000403"
     CDS             28108..29073
                     /locus_tag="U4B29_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2105375.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29172..30344)
                     /locus_tag="U4B29_000404"
     CDS             complement(29172..30344)
                     /locus_tag="U4B29_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105376.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30331..31476)
                     /locus_tag="U4B29_000405"
     CDS             complement(30331..31476)
                     /locus_tag="U4B29_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105377.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31490..32414)
                     /locus_tag="U4B29_000406"
                     /pseudo
     CDS             complement(31490..32414)
                     /locus_tag="U4B29_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32531..33610
                     /locus_tag="U4B29_000407"
     CDS             32531..33610
                     /locus_tag="U4B29_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2105378.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33642..34235
                     /locus_tag="U4B29_000408"
     CDS             33642..34235
                     /locus_tag="U4B29_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2105379.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34345..34572)
                     /locus_tag="U4B29_000409"
     CDS             complement(34345..34572)
                     /locus_tag="U4B29_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2105380.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34675..37263)
                     /locus_tag="U4B29_000410"
     CDS             complement(34675..37263)
                     /locus_tag="U4B29_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN2105381.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37283..37942)
                     /locus_tag="U4B29_000411"
     CDS             complement(37283..37942)
                     /locus_tag="U4B29_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2105382.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37991..38494)
                     /gene="trmL"
                     /locus_tag="U4B29_000412"
     CDS             complement(37991..38494)
                     /gene="trmL"
                     /locus_tag="U4B29_000412"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN2105383.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38715..39581)
                     /locus_tag="U4B29_000413"
     CDS             complement(38715..39581)
                     /locus_tag="U4B29_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2105384.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39690..40331
                     /locus_tag="U4B29_000414"
     CDS             39690..40331
                     /locus_tag="U4B29_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN2105385.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40393..41754)
                     /gene="mgtE"
                     /locus_tag="U4B29_000415"
     CDS             complement(40393..41754)
                     /gene="mgtE"
                     /locus_tag="U4B29_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN2105386.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41772..42671)
                     /locus_tag="U4B29_000416"
     CDS             complement(41772..42671)
                     /locus_tag="U4B29_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2105387.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42673..43470)
                     /locus_tag="U4B29_000417"
     CDS             complement(42673..43470)
                     /locus_tag="U4B29_000417"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN2105388.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43448..44152)
                     /locus_tag="U4B29_000418"
     CDS             complement(43448..44152)
                     /locus_tag="U4B29_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN2105389.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44283..44859
                     /locus_tag="U4B29_000419"
                     /pseudo
     CDS             44283..44859
                     /locus_tag="U4B29_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44977..45654
                     /locus_tag="U4B29_000420"
     CDS             44977..45654
                     /locus_tag="U4B29_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2105390.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45733..47541)
                     /gene="pepF"
                     /locus_tag="U4B29_000421"
     CDS             complement(45733..47541)
                     /gene="pepF"
                     /locus_tag="U4B29_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN2105391.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47717..48715)
                     /locus_tag="U4B29_000422"
     CDS             complement(47717..48715)
                     /locus_tag="U4B29_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN2105392.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48941..49594)
                     /locus_tag="U4B29_000423"
     CDS             complement(48941..49594)
                     /locus_tag="U4B29_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN2105393.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14949 a        10486 c         8772 g        15747 t
ORIGIN      
        1 gtaaaacctc aagaatatca gaatatttag gatctttact tatagatata tcattatata
       61 attcatgaac aatttgctta gcattttttt tcattttagc acttccctgg aattgctcca
      121 cctaaaaaac cacctataga cattcctgca atacaagtag ttgccttggc ccaatcgacc
      181 gtacatttat ttttagtgca atacactcca tttccataat attttccact atgagtggta
      241 cccccataga taagttgtgt ctctttaata gacaaaattt ttaaatgttt cataatctaa
      301 tcacccctaa attaatgttt ttagactatt attctaacat tcattaaatt tcaaaaaaca
      361 attccttctt gaaacgtagc aaaataattc ctgaataaga atttaatcac ttttaagtat
      421 ataaactatg tatattcttt aatatttttt gagaatgcac ttcgtacata ataaacttta
      481 atcacattag tctttcatac ctacgatcga tctctttgac tcgcacccat aaattcataa
      541 tagtttgtcg gcgtctttgg acgtttcgaa gatgagctac gcattctctt tttttataga
      601 caaaaaagat agagacgaac atggagcttc caaatttgga tatttgatcc aattttggaa
      661 gctccattta tttcgtgtct gtctttttat tttttccagc tttcgactaa ttcttggttc
      721 tctttgatcc agtcttttgc tgcttgctgt ggatcttttc cattgttgat ttccaacata
      781 actttttcca tatctttttc tgaccaatgg aaattgtcta atactttgta agcttctggc
      841 tggtcttctt tcaatccttt tcgaaccatc gtatggatct gctcttcgct tcccattccg
      901 ttttcaggat cttctaagta tttcagatca tatttggcaa acatccaatg aggtgtccat
      961 cctgtaacta caattggttc gtgttttttg atcgcttgac ctagtgcaac ggtcattgct
     1021 cctgaagaag atgtctctac tttccagtct ttcaagttgt catatttttg gatcgtgttt
     1081 tcagcagccg taactacacc agctcctggc tcgattccta tgattttctt acctgcctga
     1141 tcggttaact catcgatcga atttacatcc atgtaggctg gcactgctaa accaactttt
     1201 gctcctgtca aattggcacc taggtcttcg acttgatctt tatattgtgc atattgtgaa
     1261 ccatgagttt taggtagcca agctgaaacc atggcatctg actcgccatt tgaaacagat
     1321 ttccacatga ttgaattatc taatggcgtc atcgttacat catagcccat ttgtttcaat
     1381 acttcaccga ctacatttgt tgaagcaact tctgtatccc attcaacata agataagttg
     1441 atacgttttg tttctttggc agatgaaaaa ctccctatac caatcgcacc taagatcact
     1501 actactacag caccaatgat cagtccttga cgttttttac ttttctgtgt tgttgctcct
     1561 gcagcttttt tattgtttgg ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct
     1621 aagataacta atgcaacccc gttgacaaat ccattcccga cttgtgctct ttgcaatgct
     1681 gataatacgc cgcgtcctaa gccgggtgca ccgatcatag aggcaataac gaccattgaa
     1741 agtgacagca ttgttgtttg attgatccct gccatgatcg tacttttagc taaaggcaat
     1801 tctagtttga atagtttttg acgacttgtg ctcccgaatg aatcagaagc ttctactaat
     1861 tctttaggaa cttgtcgaat ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag
     1921 atgactgagg caaatacccc tggaaccatc ccgataccga aaaatgcaac tgctggaatc
     1981 aaataaacga atcctggcat tgtctgcata aagtcaagga tcggtttgat aatactttgg
     2041 gctttactgc tttttgccat caagatccct aatggtaccc caatgatgat cgagatcaca
     2101 ctagataaaa gaacaagtgt gacagtattc atcaaatcat tccatagacc ttggttataa
     2161 ataaaccata gaccgattaa tgtgaacaac gtaagtcctg gacgtttctt agaaataaag
     2221 aaagctgcta ccgtcaataa caagataaac aaaggagctg gaatcactaa cagtaaattt
     2281 gtaatccccg acatcactgc ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa
     2341 gttgtcgtaa accattctgt gatattttct acccagtttg ccactggtaa ttgatagttc
     2401 aatagattat tcatgttcac tcacctcgct tgtttctgct aatgcttcaa tcacactacc
     2461 tcggatgacg acacctttta attttccatc ctctacaact gcaagtggag tcggtgagtc
     2521 ataaataata ttgaaaatat cacttaccac catgtctttc ccaatcaccg tcacattttc
     2581 atctaggaag tcgatcaatg gacgtttttg cttcctagct tctaacgctt gatctgcagt
     2641 gatgatccct ttcaggtgtc tctttctgtc aacagccatc agcatgctga cttcttcatt
     2701 acgcatcctt gtcaatgcaa cgtttggtcc atcagattcg atattcgtcg tcaatgcagg
     2761 taccatgatg ttttgagccg taagcacttt ggaacgatcg acttcttcca caaattcacg
     2821 tacataatca tttgctggat tggtcaatat ttcttcacct gttccgatct gcatgatttc
     2881 gccgtctttc atcaaggcga ttctgtcccc aatccgtaaa gcttcattca aatcatgggt
     2941 aatgaaaata atcgtttttt gaacattggc ttgcaggtcc agtaattcgt cttgcatttc
     3001 tcgccggatc agcggatcaa gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc
     3061 atttgctaaa gctctggcta ggccaacccg ttgttgcatc ccaccagata actgactagg
     3121 atattggtct ttaaatgaaa gcaaactaga attttctaat gctttttctg ctttttcttg
     3181 tcgttcttct ttaggtacac cacggacttc taacccgtat tctgtatttt ccaagatcgt
     3241 tcgatgtgga aacagaccga agttttggaa aaccatattg attttatgac gacggacttc
     3301 tcgcaattct tctttgctta acttcgatac atcttctcca tcaatataga tactgcccga
     3361 agttggctca atcagacgat tcaatagacg gatcaatgtg gatttcccac tcccggaaag
     3421 ccccatgatc acgaagattt ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac
     3481 cgttgcaccg gttttcttta aaatctccgt tttcggctgg tgatcattca tcatatctaa
     3541 agcggcttgt gtctttttac caaatatctt tgttaaatgt tttacttcta cttttaccaa
     3601 acaaattctc cttttttatg taaattcatt tttctttctg aaaaaagtga ccacactatg
     3661 ttaacatcat gtagtcactt tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa
     3721 aacttggcta aatctttgta aataaaaggt ttagccaagt taatattttt ttaatatttc
     3781 acacaatcat tctgcaagct gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt
     3841 atattcattt ccaataaaat gaacgatgtc ccctgccgta aatttagcat aggggccagg
     3901 tgaaacaagc agttcttcat tatgagaaat tgcgataact gtggcgccag tcgcctgcca
     3961 gatattcaac tcgctaattg aacggtccag atgcattgct ttttctgata aagataattc
     4021 aaacggaagt aaaggattca tcttatgtat tcgcttcgtt tgattgacca gctgatccaa
     4081 taattgagaa aaatgattca gctcatcttg ttgcttctcc acacttgcca tgatttctgc
     4141 ttttgtatct tggatggatt gtacatcttg atattgcaat aggaaatctt ttgctttttc
     4201 cttcgaagca acaaatgccc cacttccatg ccgtacttcc atgattccaa gatctaccag
     4261 cacattgata gccttgcggg tagtctcagg agaagcattg tacgtactgg caagtgttga
     4321 gcgggcatgg atcttctcac ccactttata tcggttttca acgatacgtt cggctaaatc
     4381 aacagcaatc tgctggtagc gcgggcggac gatttttttc tttccagaca ttttctttcc
     4441 tctttctttt tttactttcc ctcatcttac tatgctctag caaaataagc aaaaaagggg
     4501 agaatagcct cttgcaacga ctgatcatcg cttgatctgt tcctttttta ctgggcaagc
     4561 tgtttgacag tcatcacaat tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc
     4621 acttccaaag attaacacac ttaataagaa tgtcgccatc ctattcctct ccttctaatg
     4681 acaatatagt ataaaactgg ttcttggatt ttggctgacg gaacaaaaca taaacgagtc
     4741 ctatcatcag taaaatagca agatacgtcc ctaacgctac ctgtccaccc tccaataagt
     4801 gtcccaactg ataaatcaca aaactaacta aataagctaa accgcattga tagcctatgg
     4861 cgatccaagt ccatttcttg gcattcattt cccgatggat agccccaatt gccgcaaaac
     4921 aaggcgcaca aagtaaattg aaggctaaca atgaataagc tgctgcggga gtatatgtgt
     4981 gttgtagcac ttgccagatt tctctgccat tttcggatac ttccgcatga ccaaacaaaa
     5041 taccaaatgt gccaataata ttttctttag cgaccaatcc tgtaatcgct gcgactgttc
     5101 cttgccagtt tccccaacca agcggctgaa aaacaactgc caggattcgc ccgaaaaagg
     5161 ctaagatact ttggtcttca gaaacaggtt gagcataaaa attataagaa gagctgaacc
     5221 agatcacgat actagaaaca aagataatcg ttcctgcttt tttaacaaaa gacttgctgc
     5281 ggtcataggt ttgtcttagg accgctgacc attgaggaaa atgatatgcg ggcaattcca
     5341 tgataaaagg agctgtatct ccagcaaaca aacgtgtttt tttcaagctg attccagaga
     5401 aaataattgc agtcatacca agaaaatagg cagaaggagc tacccaagat tgatgaggaa
     5461 aaaaggcccc tgctactaag gcaataatcg gcaactttgc agaacaaggc atgaaggttg
     5521 tgaccatgat cgtcaaacga cgatcttgct cgttttcaat cgtgcgactg gccatgacgc
     5581 ctggcactcc acatcctgta gcaatcagca taggaatgaa ggattttcct gaaagaccga
     5641 atttcctgaa aagtcgatcc atgacaaaag cgatcctcgc catatatccg cagtcttcca
     5701 gaaagctcaa gcataaaaac aatataatca attgcggtag aaatcctaac actgctccca
     5761 cgcctgcgat gatgccgttc aagatcagct cctgcatcca tcccgctact tgccattcag
     5821 ctaacaatct gccaacatgt tctggtaacc atgtaccaaa caatgtgtca ttgatccaat
     5881 ctgtccccat tgttcctact gtttgaatag ccagataata gatcagccac atcacaaatg
     5941 cgaagatcgg caaggctaac caacgattcg ttgcaatctg atcgattttg tcactcatcg
     6001 ataattggaa agaatcattt gacacaacac agagtgtaca tagttgggta ataaattcat
     6061 aacgttcatt gaccagaatc gtctcactat cgtcgtccaa tagtttctct gttaactgga
     6121 tcagttgctc gatctctttt tgctgagaaa ctgctatctc gtaatcctct cttaccttct
     6181 gatctcgttc aaaatatttc attgcatacc agcgtttctt gccgctttgt tctcctagta
     6241 catcttcaat ttcatgtaaa gctgcttcta aacgtggatc atatgtacag ggaacaggaa
     6301 tagaaggccg cttttttgca gactcctgaa tcttttcgat tagtttttcg attccttgct
     6361 ttttcaatac gctgattggt aaaactggta caccaagacc ataggaaagc ttttctagat
     6421 tgattttttt tccttctttt ttgatgacat ccatcatatt cagaccaatg atcataggaa
     6481 tgtctaaatc caataactgc gtagttaaat aaagatttcg ttctaaattg gtagcatcaa
     6541 taatgttgat gatcagttca ggctgttcct gaaccaaata atctcttgca atcacttctt
     6601 ctggtgtata aggagacaaa gaatagatgc cgggtaggtc ctgaatatga atgttcggat
     6661 tttttttcaa cgatccttct tttttttcta ctgttacgcc cggccaattc cctacatatt
     6721 gattcgaacc agtcaagaga ttgaacaagc tagttttccc gctgtttgga tttccgacta
     6781 acgcgatatt cattcttttt cctccctttg tatcaagatt agttcggcct ctgactttcg
     6841 taacgtcagt tcataccctc gcactgtcag ttcgattgga tctcccagag gggctagttt
     6901 tcggataact atttttacat ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc
     6961 gccttgtccg tggattgcta cgacgatccc agtctctcct gcttttaatt gatcaagaga
     7021 agtccagctt tcagaggaag cagtcttttt attgacaaag atttgaccta gtatttcccg
     7081 acttactgcc actcgatgac tttttaccag taaaatggca ttgttgcctg ataaattgac
     7141 gacgacgatt ttacttccgg caacgatccc gatattttgc aaatggcgtt ttgttttttc
     7201 ttctgtttcg attcgttcga cttgataaac gaaattctgc tttgcctgcg ctaaggtctg
     7261 catattgatt cctctcttgt gaaataaatc atgtattaaa atcattcatt atatgttcta
     7321 attttcttac gtgttacgca aataaatgag aatcattctc acatagaata ctctcggtaa
     7381 ttgagaatgt caattatact tgtgatatta tgagcgaatc tttagtattg atagataaaa
     7441 atgagaaaaa aatatttctt catagttttc cacaattttt tctctattaa atttgctatt
     7501 ttcccacacg atacttgttt tcagctcttt tttcaaaaac acaaaactta gtattccaca
     7561 gtttccacaa cacaatatgt agtgtttgtt gtttgacttt tttactaaga gttttatact
     7621 atctattata gataataaga atgtgttaga ggaggcactc accatgaacg taaaaatctt
     7681 ttctaaaaat aattgtatgc aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat
     7741 tgcttttgaa gaagtcaata tcgatgaaca gcctgaagca gtagactggt tgaaagcaca
     7801 aggattccaa agtgttcctg tcattacttc tgatgcagaa acagttatcg gattccgtcc
     7861 tgatcagttg cgtaaacttg ccagctaaca tgactaaaag aatcaaaacg tgatacatgg
     7921 atttgaaggg cttgagacag caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag
     7981 cttaaaaaca atgcaatgat ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc
     8041 gtacgtatga gtcttaaaaa tttacaagat gtcagttatt tcaagctgaa taatgaaatc
     8101 aatcggccag tcaatggtca aatccctttg aataaggata aagaagcttt agttgccttt
     8161 tttgaggaaa atgtcaaacc aaatacgatg acttttctat ctattatgga aaaaatcaat
     8221 tacttgatcg atcaagaata tatagaagca gaattcattc aattgtattc accatcattt
     8281 attgaaaagc tatatcaatt tttggacgag caacattttt ctttcaaatc ttttatggct
     8341 gcttacaaat tttattctca atatgcgttg aaaaccaacg acggttccgc ttatctggaa
     8401 acttatgaag atcgagtagc cttcaatgcg ctttactttg cgaacgggga tgaagaatta
     8461 gcacttgctc ttgcagacga aatgatccat caacgatatc agccagctac accttctttc
     8521 ttgaatgctg gacgtaaacg tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact
     8581 gacgatatga actctatcgg gcgctcaatc aactctgctt tgcaattgtc ccgtattggt
     8641 ggcggcgtag ggattacttt atcaaatctt cgtgaagccg gcgctcctat caaaggatat
     8701 gagggtgctg caagtggtgt cgttccagtc atgaaattat tcgaagatag tttcagctat
     8761 tctaatcaat taggtcagcg tcaaggagct ggcgtggttt atttgaacgt tttccatcca
     8821 gacattattg ctttcctttc agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa
     8881 actctttctc ttggcgtgat cgtacctgat aaattttatg aattgacacg taaaaacgaa
     8941 gatatgtatt tatttagtcc atatagtgtc gaaaaagaat atggtgtccc attctcttat
     9001 gtaaatatta cagctgagta tgatcatcta gttgcaaatc caaacattcg caaacaaaaa
     9061 atcaaagcgc gtgatcttga aaatgaaatc tcaaaattac aacaagaatc tggctatcct
     9121 tacgtaatca atattgatac agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg
     9181 agtaatttat gttcagagat tttacaagtt caaacgcctt ctgtgattaa cggcaaacag
     9241 gaatatgaag tattaggaac ggatatctca tgtaacttag gctcgacaaa tatcgtgaat
     9301 ttgatgcaaa gccctaactt tggtcaatct gtgcgtacga tgactcgtgc attgacattt
     9361 attacagatg catctgatat cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat
     9421 catacgattg gattaggtgc catgggctta cacacttatc ttgcaaaaga acaaatggaa
     9481 tatggctcag aggattcatt agactttaca aatatttact tcatgctgtt gaattattgg
     9541 acacttgttg aaagcaacaa tatcgctcgt gaaagaaaac aagttttcca taattttgaa
     9601 aaatcagctt atgctgacgg cacttatttc gaaaaatacg taactggaga attccagcca
     9661 aaatcagaaa aagtcaaagc attgtttgaa ggcattttta tcccaactgc tgaagattgg
     9721 aatgctttga aacaagcagt aatgaaagat ggactgtatc atcaaaatcg cttggcagta
     9781 gcaccaaatg gctcaatttc atatatcaac gatacgagtg caagtattca tcctatcact
     9841 cgcatgatcg aagaacgcca agagaagaaa atcggtaaaa tttattaccc agcgccttat
     9901 ctatccaatg agacgattcc gtattacact tctgcttatg atatggacat gcgtaaagta
     9961 atcgatgttt atgcaatggc acaacaacac gtcgatcaag gcatgagttt gactttattc
    10021 atgcgttctg aaatcccaga aggcatttat gaatggaaga caacaacgaa gcaaacgaca
    10081 cgtgatttga atattttaag acattacgct ttccacaaag gaatcaaatc catttattac
    10141 gtacgtacct ttactgatga tgcagaagaa atcggaagca atcaatgtga aagctgcgtg
    10201 atctaatatc atagatagat tcgcttattc atattttaat taaaggagca ttctcatgtc
    10261 agaaacatat tatgaagcaa taaactggaa tgaaattgaa gatatcattg ataaatccac
    10321 ttgggagaag ctgacagagc aattttggct agatacacgt atcccacttt caaatgacct
    10381 agatgattgg cgtacattgt ctcaattgga aaaagataca gtaggacatg tatttggcgg
    10441 tttgacactg ctagatactg tccaatctga aagtggcatg gatcagttgc gtaaagatgt
    10501 ccgcactccc catgaagaag ccgttttgaa caatattcaa ttcatggaat ccgttcatgc
    10561 caaaagttat tcttctattt ttagtacact gaatacgaaa aaagaaatcg aagaaatttt
    10621 tgactggaca aatacgaatc cttatctgca aaaaaaagct gagcgtatca atgaaatcta
    10681 caaacacggt acaccgctag aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt
    10741 ctattcaggt ttttatactc ctctttatta tttaggaaac aacaaattgg ctaacgtagc
    10801 agagatcatc aaactaatca tccgagatga atctgtacat ggaacctaca tcggttacaa
    10861 attccaatta ggattcaacg agttacctga agcagaacaa gaaacgttaa aagactggat
    10921 gtacaatctt ctttatgagc tttacgaaaa cgaagaacgt tatacagaag aattatacga
    10981 tccaatcggc tggacagaag aagtcaagac attcctacgc tataatgcca acaaagcatt
    11041 gatgaaccta gggatggatc cactctttcc agatactgcc aatgatgtca acccaatcgt
    11101 aatgaacggg atttcaacag gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg
    11161 ctatttgtta ggtcatgtag aagcgatgaa agacgacgat tatttgattg gattggaata
    11221 aggatttatt ttaattcaaa aatttttcga aaaataaaaa gagtttagcg tacagatatt
    11281 ttaaatgtac actgaactct tttttatgct tattcacttg tttcttccag cacttttgga
    11341 ctgtctaact tttccgaaac atgttccatt cttctcaata aatagatttt ccggatattt
    11401 aatattatcg tcttaatcac tgcatagaca ggtatgcaaa tcagcatgcc taatattcct
    11461 gcgacacttc ctgctccaat caataccaag atgatcgtca aaggatggat attcattgat
    11521 ttcccaaata acaacggctt gatcaaattt ccatctaatt gttggatcac taaaatcgac
    11581 aacgccatat acaaagcttg aaatggagag ataaacaaac cgacaatgat tgctggtacc
    11641 gctccaataa aagggccgac atacgggata atattcgtga ttccgcaaat gaccgcaagc
    11701 aacaagctat aaggctgatc gaatattctc attgccaaaa agctcattac accaataatc
    11761 aaagcatcta agatcgtact gctgatatag gcagataatg tttcgtttaa ttcttttacc
    11821 gtttggcgta actctttacg aatcgctttc ggaaagaagt gagacaacgc atcaataaat
    11881 ttgtgcccat ccttgaacat aaatagcaaa ataaatggaa cggtaaataa caaaataaaa
    11941 aattgcatca taaatgaaat gatttttgag acacttgcag ataaactgac taatatgata
    12001 ttgaacaaat tactaagaga taaatttgct gatgctaatt gttcttccaa attgaaattt
    12061 ttaaactctt cccgctgcgc caattcattt aaccatttgc ttgtttcatc tgcatacatt
    12121 ggcaagctct gggtcaattg aattgtctgt tccattaact gaggcactac attcatgaca
    12181 gttaaaacaa ctaaaatgat tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg
    12241 atccgatgct tttcgagaaa aacaacgatt ggatcgaaca tataatagat aaaaccagca
    12301 acaagcaatg gtaaaaaaac tgccgaaagc attttcaata ttggactaaa aatattcggc
    12361 atttgtaaaa taaagtacac gccgatgatc gtgagtacta gctccactgt ccagaaaaat
    12421 aattttgatt gtttaaaacg tgattgcata catctcctcc aaggtccttt gatacttcta
    12481 ttctatcatt taacaaagag ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa
    12541 aaataaacat attttaatga gtagacgagt acttaaacaa aaacacggtt tggagtagtg
    12601 acaaaaatta tactgcagtc cctttttcca aataaacggt gttcaaaagc ttgcatcttc
    12661 gtaataagcc cagccaaacg aaaaatataa ggaactattt ttatttggct gggcttatta
    12721 cttgatgtag gacatctatt ttacaacctc agatcattta ttgcgcaata ctgactaaat
    12781 gcaactttgt gatatttttg tgtacggaca actcctcgat cacatcggca taagtgagcc
    12841 cttttggaag atcaatcgta tagatatttt tgtacactcg gtagtcatca acgaatttta
    12901 cgtcaaaatt cacatcttcg attacgatat ttttctcttc aaaataatcg gtaagaaaat
    12961 ctttggtttc ttttctatgg ataaattgga tttctaactt tttagtcgta ggtacatgga
    13021 tcacgcgttt cactactgtg agtgcaatag caatcccgat aaaactgatg atcgcaatgg
    13081 aatagtaacc catgccgatg gcgatcccta atccagcaat tgcccataaa gaagcggcag
    13141 tcgtaagtcc cgtcaccgat tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga
    13201 caccactcac cacttgagca atcagccttg cttcatctga acgaatcgtt ccagcaagct
    13261 gtggatactc taaagcatct cttaatgcac cactagcgat ctcaacttgg atcaatgcaa
    13321 tgatacatgc tcccatacaa actaaaatat gcgtacgcat acctgctgga cgatttttat
    13381 attgtcgttc aaaaccgatt gctccgccaa atagcatagc gatccctaaa cgtaaaataa
    13441 tctctggaat tgtcaatacc atatcattca tctaaacacc ctccagtcca atatatcatg
    13501 tctgaaagga gcagacaaaa aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac
    13561 aaaatttttg tcatagtcct ttttatgaat aaacggtgtt caatcagctg accctcggta
    13621 ttactcaaca aacggcaaaa tatggggagc tatttttcct ttgtcttctc taatactcag
    13681 gtcaaaacgc tgatttcaca acctctttag cctactattg aaaattacaa cttacttgct
    13741 gcttcttcat cgatgatcac aacaacattg tcgtgatttt gcaatacact tgctggcaaa
    13801 tgattcgtta caggtccttc aatcatccct ttgattgcat cagctttgct ttcgccaaat
    13861 gccatcaaga cgatttcttt acttttcata attgaaccga ttcccattga aacagctttt
    13921 gttggtacat cttctgcttt atcgaagaat cgtttgtttg cattgattgt tgattctgtt
    13981 aagtcaacta ctgaagtcgt tccgtcaaat gaagcaccag gttcattgaa accgatatgt
    14041 ccattttgcc cgattcctaa gatttggata tcgattggat gtgcatcgat gatttgatca
    14101 tactttttgc atgctgcttc cagatcttct gcttttccat ctggtacaaa cgtttctttg
    14161 aatggttttt tatcaaataa ttggacatcc atgaaatgac ggtagctttg ttcatctgaa
    14221 ccgcctagtc caacgtactc atctaagttt acagaagtca tttcactgaa atccaaatcg
    14281 cttgaagtca tttctttata taaagtaact ggtgtgcttc ctgttgccaa tcctaatact
    14341 tttgcacctt tgttcatacc ttctttgatg atttcaaatg ctttttttcc gccttcttca
    14401 gcgtttttta cacgaatgat ttccatagtg attgctcctt tatattttgg tatagcccaa
    14461 ttatagcatt ctttttttct ttttacaata attagtatag accaaattgc atttttttca
    14521 ttttcttgac aataaatagg aatccgtgtt aggtttcata agatggttca atcaaacaat
    14581 ccatgttgca tgaaaaccaa gcaagtgatt cactgtggac gctttatgtc tgcagttttt
    14641 ttatgaaaaa ggagttatta tgagtatact gacattaaac aacattaccc agcaatttgg
    14701 cgacaaaatc ctctatgaag gtgtcgatct gcaattaaat gctggcgaac atcttgggct
    14761 gattggacaa aacggagccg gtaaaagtac attgattaaa atcatcactg gtgagatctt
    14821 accagatgac ggacagatcc attggcaaaa aaatattcac aaaggttacc tcgatcaata
    14881 tgtggaagtc gatgaaacat taacaatcga agaatttcta aaaactgcgt acgcaaaaga
    14941 atacgaaaag gaagaaaaaa ttgcttttct ttaccaaaca tatagcgaga ccttcgatga
    15001 ttccttgctg gaaaaagctg ggaaactcca aacggaatta gaccaaggag tgttttatca
    15061 aatcgacacc ttagttgctg agatgagcag cgggttgggg atcgatgtct taggtttaga
    15121 ttcactgttg aaaaatctaa gtggtggaca acgttcaaag gtgattttag caaaactact
    15181 tttagaaaac ccagatgttc tgatcttaga tgaaccaacc aatcacttag atgatcagca
    15241 tatccaatgg ctgacgcagt ttcttcagga ttttgacggt gcgtatatcg tgatttccca
    15301 tgaccaagaa tttcttgatc agatcaccac ccatatcgca gacatcgagt ttggaaagat
    15361 tactaagtat actggccatc taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag
    15421 ctatttgcgc caatatcatg ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg
    15481 aaaatacaaa gccggttccc gatcaacgat ggcaaaaagc cgacaaaaac aattagataa
    15541 aatcgaacgg ctgacgcctc cagcttcagc aagtaaagct gtctttgatt ttccattctc
    15601 gcctattgtc acgacattag cagtggaaac aactgattta gtcatcggat atgatcgacc
    15661 gctacttgca cccatcaatt tgaccatccg ctttggtgaa aatattgcca ttcgaggctt
    15721 caacgggatt ggaaaatcta ctttattaaa aacactgatc ggtgagattg aaaaaataag
    15781 tggcgatttc cacttcccag ataacacaaa aatcaattac ttttcacaag atctgtactg
    15841 ggaaaatcca ttagaaacac cactacaata tttaagcagt caatttccaa aagcgactat
    15901 caaagaatta cgtcgccaac tagctaaagc agggttagtt aatcaattag caagtgaacc
    15961 tctcactaca ttaagtggtg gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa
    16021 ccaaggtaat ttactgattc tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga
    16081 aagcttacaa gacgggatac agaaatttcc aggaacagta atcatcgtct ctcatgaaca
    16141 agaattttat caagatcttg tgcaacgagt cattgagatt gaaccaaaat aaacgtttga
    16201 ttgaatagtt gaaaaaaagg agctgtgaca aaagttttgt cacaactcct tcttctgaat
    16261 aaatggtggc caattagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag
    16321 ctatttttat ttggctattc ttattacttg atgcagaaca gcttttcaca acctcttttc
    16381 gtctattttt tattttcgag atttctcttt cctttaaaaa caactgtttg aaagaagaac
    16441 gcacatcctt gaaaaacaag cccgctaacc gttacaccta accagccata ctgctgccac
    16501 attgtcgtcc ctagcaatga accagccgca cctccaataa agtacataaa cataaataca
    16561 gtattatttc ggttgcttgc tgcttcacct agcccctgta ctcgtgtttg attggcgact
    16621 tgaccaaatt gattcccgac gtctaataaa ataatagcta aaagcaaaat ggccaaatgg
    16681 cttccgccaa ccaataaagc aataaagctt attgtttgca taacaagtcc catatacacg
    16741 atttttcttt cagaataaga atctgccagt cgtcccacga taggtgcagc aactgctcca
    16801 gacaaaccaa aaatcgctaa tatccctgct tcgaatgttc cccaattgta ggcgggactg
    16861 ctgatataaa aaatcaacgt agaccaaaag atcgaaaatg ttccaaacat gaaaaatcca
    16921 ctgactgccg cttctcttaa tagacgttga gaagcaacta aatgcggcaa acttttaagt
    16981 gaatcaaggt atgaaacagc tgtactactt tgctggacag aacgcggcat tttccagtaa
    17041 agcagaagtg taagaattcc gactgcacat aaagcaaata aatagatcat ccgccaagaa
    17101 aaaagactgg caacaatacc agataccgtc cgagaaagca aaatccccac gagtaaaccg
    17161 ctaagcaatg tacccatgac cttccctctt gcttttggtc ccgctagtac cgcaccgtaa
    17221 ggaatgatga tttgcggcac gatcgaaagt aatcctatta aaaaagaagc gcatgcaaat
    17281 aacgtaaacg aaggcgcaaa aaaagcggcc aacagagaga cggcagataa cgctgccatt
    17341 cgaatgatca attttggacg atccaccacg tcccctaaag gaactaaaaa cagtaaccct
    17401 aacgcatagc ctaattgggt caacatcgtc aatatcccga ctgcacttgt gctaacagat
    17461 aaactagctg ctacttttgt tccaatcggc tggatgtaat acatattggc aacaacaacg
    17521 ccacaagtga ttgataatag taaaataagc gaagttgtta aaggtttttt ttcattactc
    17581 ttcattgatt tcccccattt cctatcttag tttacaactt atggaaaaga ttggaaatag
    17641 aaaaatacaa ctattcgcca cacttattat ttgtgctttt gttttccttg ttttttcttg
    17701 cgatctttat aaatgaagac aactaaccct actagcacag taacacttgc aaccaacgtg
    17761 actgcagttt tgttatatcc gtgaaacaga gcttctccac cacacgcata caacacagac
    17821 gaagggatga ctccaatcac gatcgacaat atgatttgct gtagaggcaa tttcatcgca
    17881 tctgcagcaa aattgaccat agaagatgga ataaccggta tcatatagcc taacattaca
    17941 cctatttttg gatgtttcat ttgtcggatt gcttgcaccc aatgattcgt ttcggttttc
    18001 ttatctaaaa attttaagcg atgcataagg aaaattgcag tgatgttacc aagagcatta
    18061 cccaaaatgt tgatgatcgc acctgtcagt ggtccaaagc tcaaaccagc aatgactcca
    18121 actactgaag taggaatacc tggaacagca cacatcaccg ctgtcaatac gatcaagagt
    18181 atcgatgcaa aagtcccatg cgaacgaata tgtgcgagta gctctgcctt actcgctctc
    18241 ggatttaaaa ataattggat gtctgtctga tattctaaat acaaccgata gcccactacc
    18301 agtatcagaa tgattcccaa cgagatcaat atcaatcgga ttattttctt tttcatattt
    18361 cctcctgaaa aaaatctata ttcattaaat aatagtttaa cataaaaaca aaaaagcacc
    18421 gaaatccaac ataggatctc agtgctttta aatagtaaaa attatttaac agttacagat
    18481 gcgccaactt cttcaagttt agctttcaat tcttcagctt cttctttaga tacgccttct
    18541 ttaactggtg caggagcgcc atcaactaca gctttagctt ctttcaagcc taaaccagtt
    18601 gcttcacgaa ctgctttgat aactttaact ttttggtctc cagctgcagt taattctaca
    18661 gtgaattctg tttgttcttc agcagcagca gcgccaccag cagcagctac agctacagga
    18721 gcagcagcag atacgccaaa ttcttcttca atagctttaa ctaggtcgtt aagttctagg
    18781 atagttgctt cttttaactc agcaacgatg ttttcaatgt tcaatgccat tataatttcc
    18841 tccgtttaat tgttcttttt tatttaatgc tttatgcggc aattaagctg catctccatc
    18901 ttctttgtct gccactgctt tgacagcgta agccacgttg cggactggcg cttgtagtac
    18961 agatagcaac atagatagaa gtccttcgcg atttggcaat tttgccaatg ctgtgatttc
    19021 ttccaatgaa gagactttac cttcgatgat accgcctttg atttctaatg cttttgcttc
    19081 tttagcaaat tcgtccatga ttttcgctgg tgcgactaca tcttcgttac tgaatgcaac
    19141 agctgtaggt ccagtgaata cttcgtccat gccttcaagg ccagcttttt tagctgcacg
    19201 tgaaagaata gaatttttga taactttcat ttctacgtta gcatcacgaa gttgtttacg
    19261 taaacgtgtt acttcatcaa ctgttaatcc gcggtagtca acgactacta ctgatgcagc
    19321 tgctgtaaat ttttcgctta cttcatcaac gattgctgct ttttttgcga taattgcttc
    19381 actcattttt gagtttcacc tcctgaattt tgatggaaga gttttttgca ctgaaaaggc
    19441 aagtcacttt tatagttact ttgctcaacc agtattattc atcacctatt cacttcgttc
    19501 atagtgattc tactcgcttt gctcgaaagg aaactccttt cgctttgctc aaccagtatt
    19561 gttcatcatc tcttcacttc gttcatagtg attcacaata aaaaactcta tgccaccgca
    19621 gacatagagg gactattgaa tatattcaat aaacgtcctc ggtaggaaat taaggcttga
    19681 tgccacctac tgtcttcggt acaagttaat tattaacctc aactagctta gcatgctaaa
    19741 ctagttgagt caagaattat ttttcagttg gatcaatcaa cattctaata tttaattaga
    19801 aagaagcttg gtcaacatgg ataccaggtc caaatgttgt tgtgatcgtt acatttttca
    19861 tgtattgacc tttagctgct gatggtttag cttttagcaa tacatcatgg attgctgcaa
    19921 agttttcaac taatttttca tcgtcaaatg aaacttttcc gattggtaca tggatatttc
    19981 cagctttgtc cacacggtaa gttactttac cagctttcac ttcgtttact gcttttgtta
    20041 cgtccattgt aactgtacca gttttagggt ttggcattaa gccttttggt cctaatactc
    20101 gtcctagacg acctacagta gccatcatat caggtgttgc aacaactaca tcaaagtcaa
    20161 accatccgtt ttggattttt tgaaccatgt cgtcgtcacc tacgaaatct gcaccagctg
    20221 cttcagcttc ttttgctttg tctcctttag caaaaactaa aacagtttgt gttttaccag
    20281 ttccgtttgg taatacaaca gcaccacgga tttgttggtc tgctttttta gggtctacgt
    20341 tcaaacggta tgcaacttca actgttgcat caaattttgc aatgtttgtt tcttttgcta
    20401 aagctactgc ttcggcaaca ggataaactt tacttgaatc aacttttttt aatgcatctt
    20461 gcattttttt gctcttttta gccattttgg ttcctccttg attgtggtta taacggttgt
    20521 acctcccacg tgttctatat ctttcaatat agagccaact gagaagctgc tgtcttaggg
    20581 tgtatagatt attctacagt gatccccatg cttcgtgcag ttccttcaac catgcgcatt
    20641 gctgcttcaa cgttagctgc gtttaggtct tccattttta attcagcgat ttctcttact
    20701 tgatcgctag aaactttcgc aactttattt ttgtttggtt cgcctgaacc tttttcgatt
    20761 ttagctgctt ttttcaatag aacagcagct ggcggtgttt ttgtaacgaa tgtgaatgaa
    20821 cggtcttcgt atacagaaat aacaaccgga atgatcaaac ctgcttgatc agctgtacga
    20881 gcgttgaatt cttttgtgaa tcccatgata ttgatacccg cttgacctag tgcaggacct
    20941 actggtggag ctggagttgc tttacctgca ggaatttgca atttaacgat tttttctact
    21001 ttctttgcca cgagacatac ctccttaagt ccgtgatgtg gttaattgga gatgcatatt
    21061 tctcctccca cgttttatct gatctgaaaa aatccagaca aataatgtgc aaaacaatgc
    21121 acactggtac agtatagcaa atttatttgt tttttcaaga tttttttctg ctatttttac
    21181 attttgcttg aataaacgct aaatttattc taaaaaaaca acaatttttc gttctcattt
    21241 cttcttttat gttacacttg cgctagcatg gttcaacaga tgaaagcaaa caataacacg
    21301 gttttttaga cccttgtaca aagattccta cataacaaaa tgaggtggag aaaatgaaag
    21361 atttaaggta tagaagtgtc tttgatatta ttggacctgt gatgattggt ccaagtagct
    21421 ctcacactgc cggagctgct agaatcggga aaatcatacg tagtatattt ggcgaacagc
    21481 ctgattctgt agatatttat ctatatgaat catttgcaaa aacctatcgg gggcacggaa
    21541 cagatattgc attagttggc ggcctgttag ggatggaacc cgatgatgaa cgtttagctg
    21601 attctttgaa attggcatat gaagccggta tggaagtctt gtttgttcct aagagtgaaa
    21661 aagccgaaca tccaaactct gtaaagatgc ttctaaaaaa aggaacacga aagctttcag
    21721 tcactggcat ttcgatcggc ggtggcaata tccagatttc tgaattgaat ggattcaaaa
    21781 tttctttaag catgggtact cctaccttga tcgttgtcca ccaagatgtt ccaggtatga
    21841 ttgctaaagt gactagtata ctttctgaga ccaatacgaa tatcggcact atgacagtga
    21901 cacgggaatc aaaaggtgaa aatgccatta tgataattga agtagatgat cctcaagtag
    21961 aagaaacggt aaaaagactc aaacagctgc caaatatcga cagcgtaaat tatttcgatt
    22021 aaggagctaa aaaaatgttt ttatcgattg aagaattagt agatcaagca aaagcttatc
    22081 caagtgtagc agaattgatg attgcagttg aaatggaaat gagtggccgt tcccgtagtc
    22141 acatcatcga gatcatggaa aaaaacctct cagtaatgga acaatcaatc aacgagggaa
    22201 aagaaggtgt tacctccgtc acaggaatta ccggtggtga tgcaaaaaaa ttagatgatt
    22261 atttaaatga ggcagacttt ttaagtggag aaacaatctt aaaagctgta cgcaatgctg
    22321 tagctgttaa tgaagtcaat gcaaaaatgg ggttgatttg tgctacaccg actgccggaa
    22381 gcgcgggtgt tgttcctggt gtgctgatgg ctgtccgaga tcggcttgat ttgacacatg
    22441 aacagcaatt ggaattctta tttaccgctg gcgctttcgg gttagtcatc gcaaacaacg
    22501 cttctatcag tggtgcagaa gggggatgtc aagcagaagt tggttctgca agtgcaatgg
    22561 cagcagcagc tttggtttgt gcaaaaaatg ggactcctca tcaagcagca caagcagtag
    22621 ccattacatt gaaaaatatg atgggactga tttgcgatcc cgttgcaggc ttagttgaag
    22681 tcccgtgtgt caaacgtaat gctttaggct cttctcaagc ttttatctct gctgatatgg
    22741 cattagcggg tatcgaaagt gtcattccgc cggatgaagt cgttgctgca atgtaccaag
    22801 tcggacgtca aatgccgtcg attttcaaag aaacagcaga aggtggctta gctgttacgc
    22861 caactgctaa acgacttgcg gaagagattt taaataagag ggcgtgataa agctccttaa
    22921 tttcgagtat taagagatta gttatcttca taacaaaaaa cgaagagagg actaattgcc
    22981 cttctcttcg ttttttatta atccatcaat ttcgtatatt ttacttcatc gatcgttttt
    23041 gttcctgcaa gttgcatcgt gatcgacaat tctttgttca ggtgttcaaa aacagatgtt
    23101 actccttctg ccccacctag attcaaccca taaatcactg gtcggccaat cgcaactaag
    23161 tcagcaccac ttgccagagc tttgaagaca tgctctcctc gacggacacc agagtcgaaa
    23221 atcactggta cacgtttgtt gactacttcg gcaatttttg gcaatacttc aaaggacgca
    23281 ggacctccat ctagttgacg tccaccatga ttggataccc agataccatc tgcccctgca
    23341 gagatagcga ttactgcatc ttctggcgat tgtattcctt ttacgataac tggaagatgg
    23401 gtgatttctt tgattgtttt gatgtcatct ggtgtcaagc cttgttttgc agctgcataa
    23461 atttctgcga ttccttttcc ttcgccatta ccacttgcac tttgttcgct atacgcagct
    23521 aagttaggca ttggtaatgg aaattggaat tgattgatga cgtcttcttc acggtaacca
    23581 ccgagcgttg aatctgccgt taaaatgata gctttagccc ctgcttctac tgctttgttc
    23641 aaaatgaact cattgaatct gtcgtcttta ctcatatata attggaaaaa ctgcggtgcg
    23701 cctggcgctg catcggctgc gtctttgatt gtcgtatttg cataagtgct gatggaaaag
    23761 attgatccag cagcagctac tcctttagct gtatcagctt cccctttttc atgtgccaat
    23821 ccttgggccg ctgatggcgc ttggataatc ggtgtatcta attcaattcc gaaaacagat
    23881 gtatgcaaat cggctgagtc gattccgcgt aatatccgtg gcatgatttt ctttgtatta
    23941 aaagaagtcg tattttcttt catcgtccac tcatcttcag aaccaccgcg aatatagcca
    24001 aaagctcctg cttccatgcg ctcttttaca cgaccttcta gttaggcaat gtttacaata
    24061 tcgattggat gttcttcatt acttgtatga taagtcatag tagatccttc tttcatcttc
    24121 ttgattacat ataaatcata ctaaacttac aaaaagtaag caaacattta ctctcagata
    24181 cttttatcgc tattctttcc aaataaatcg acttacaagc tgatctactt cttggttttc
    24241 atgaagcata ctatgagagg cattttcacc tgtcactact ttttcctcta tttcatttcc
    24301 gcgttgcttc aacaacgcat agaccgctaa tgcactgttt aacggtactg ttccatcact
    24361 caagtctgtt tcatctagtt gaccagcaat caagaaaaat cgagtggtta taggaatatt
    24421 accgatcatt tgctggtaat cttgatagcg gttagaaacg acagcaggcc cctttgctaa
    24481 ctcatctgtc agagactgcg cactatcatc gacaaaatca ttaaaaggcg cgccaattgc
    24541 tgttaatgtc tgaacttgtg gcaatgacgt atcctgtcca tacgttccta aatagcgtag
    24601 tgcactaact cctcccatgg aatgccccac tatgttgact ttatctatgc cgtagttttc
    24661 tttcagatac aaaagacaag ttttgatcca ttctgcttga ttccactcgt tatttttatt
    24721 atcttcaaac aatacttgga ccatcggatt gtctgctttt ccagacaaat ctcctgttgc
    24781 ttgtatttgt ccatctgttc ctactgttaa aaccaattct tttactgctt gctgattttt
    24841 ttccaaacgc cgcaacatcc ctttaaagga attttctgtc ccgctgtagc cgtggaaaaa
    24901 aagtgttgga atagtggttt tttctatcac tttactggat tctgccgttt tttctttatc
    24961 agaaacttca gtagtattta tcttcgtcgt ctggcaacca cttaacgcaa acaaactgat
    25021 aaggactaaa gctgctgcag ttgcacgttt gcataaattt ttgattggtt ctgtcataca
    25081 cctcttcctt tccatataga aaaaagaaag acttgaaagg ccaatcatat gatcgacatt
    25141 tcaagtctct ttattaatcg attttatcta cttgatcgaa atcaagttct gtactggttt
    25201 cacgtccaaa catatcgatg ttgactttca atttttcttt ttctccgtca acttctgtta
    25261 cttgtccttc taatccagca aatgcgcctt cgatgatttt cacgacttcg ccaacttcta
    25321 cttctagatc attttgtcgc aggctcatac cgatagaacg caagatatga ttgacttctt
    25381 ctggaagtaa tggtgctggt ttgcttccgg ctccgtgtga accaacgaat cctgtcacac
    25441 caggtgtgtt gcggacaaca taccatgaag cgtctgtcat gatcatttca acaagtacgt
    25501 atcctgggaa tgttttatga acgatttctt tttctttccc atttttgact tcagtttctt
    25561 tttcttcagg aacaacgaca cggaaaataa agtcggccat tttcatactt tgcgcacgag
    25621 attcgatatt tgcttttact ttgttttcat atcctgaata tgtatgcaag acataccagt
    25681 ttttttcaaa agattccatt gaaggctcct catttcattg taaaataaaa aaacctcagt
    25741 acacccgaag tttttctttc gcttcattat agcactaaaa gtacgttgtg gctagcgagt
    25801 tattttaaaa tccagccaaa taagctttgg atggctgtat ccattacgta aaacatcaat
    25861 ccgaaaataa tagaagtttc gatgactact aacgtatctt tacggagttg ttttctgctt
    25921 ggccaagtta cttgtttgat ttcatctttt acgcttttta gaaatttcat gagacaacct
    25981 cccttgctac tgtttatttc gtttctttat gtaatgtata ttgattacaa tatttacaaa
    26041 acttattgat ttccaaacgt tcgccgcttt ttccttcact gactgatttt gtgtagttgc
    26101 gagacccaca aacggaacaa gcgagtgctg cttttttctt tgccatttac ttctgccccc
    26161 ttgctgagaa tcagacttat acttcataac agtatcacgt aactttttac ttgtcaattt
    26221 gtgaaataaa ctatctattg ttttggataa aattatgata tgattaattt gaaattaagc
    26281 taaaatgaga caattggagg tatagcgatg ctattaaaaa cacttgtata taagaaaaaa
    26341 gatctgacaa ccgttacaga gactagtaca ttagaagaag cattaaaaat tttagaaact
    26401 tccggatacc gttgtgtgcc gatcttagat tcttcaggta atattttccg tggaaacatt
    26461 tacaaaatgc atatctatcg tcataaagca aatggcggtg atatgacgct tcctgtcact
    26521 catcttttga aaaacgctac aaagttcatt tacctggata cttctttctt caaagtattc
    26581 tttacgatca aagagcttcc ttatattgct gtccttgaca gtgaaaatca tttttatggt
    26641 attttgacac atagtacgtt gcttaatatg ctctctcaat cttggagcgt agaacaagga
    26701 agctatgtac tgacaattgc ttccacagga aagcaaggag atttggctgc tattgcaaaa
    26761 atcatttcaa aacacagcag cattgccagc tgtattactc ttgatatcga aaaagacgaa
    26821 tacattcgac gtacattgat cacgctaccg gctaacactg aagcgaaagt atgtgaaacg
    26881 atcgtcaacc acttagagaa aaagaattat aaagtggtag aaattgaaaa tttgcaaaac
    26941 caagaagcat aatcaaaaag cgaaaaaaga aaacagctaa tctgctttct tttttcgctt
    27001 tttataatta gacacgcttt tcaaaatcaa gcgcgcgatc taaataatac atcaatggtg
    27061 ctgaaacagc catgacgata aactcactta atgcaagcgt gccataggtt gtccaaaatg
    27121 cttggccacc tgttggaact aacatataag caatcagtcc catgctgatg gtaaagaaga
    27181 tcgtgttcaa tactaaacgc atctttacat ttttcacttt gttttgcaat aaagctgtca
    27241 ggcctaaaga aagcaatgtt tggccgccac caaaaaccac atccatgata ccaaaaccaa
    27301 agaaggcatt atagacgatg accccgccta aaactcccca aagcaattta cggttaaaga
    27361 caactaaatg attcaaactt tctgaaatac gaaactggat cggccctgag gatacaggcg
    27421 caactaaaat agttaacgct agataaagag ccatgatgat gccatttaca acgattacgg
    27481 ataaccgttg tctgcttttc gttttttgca ttttctctcc tcttttctcc tagttttttt
    27541 aacgcgggat ggttgatgaa ccgcggttat gtgaaacaaa caataattat aacagacttt
    27601 tcatttattt caatccgtct tatctgcttt taaaaaagca aaagcttaaa caatgtaaac
    27661 gtttgctaaa aatcgtggga tatggtataa agtaagtgta aggggtcata agaattccca
    27721 gtaaagcaga cgaagtagga tctgcaagaa cagaattgtt gtgaaactta aataaataaa
    27781 aaaggatgtg ggaatttgag caacctctta ctagagaacg gtctagcaag cagctgaaga
    27841 tctaagtgga catgccgaac cttggaactt ctccttacgt aagtaatgga tcatgcgctt
    27901 gctaggcttt tttgtgtttt tcggtcttcg gacctttttc attttcctag acttctgcta
    27961 taataaccaa gaattaattt tgtaacagat ttttttgaaa aaacaagttt ctttcattca
    28021 aaaaatagaa aaagcttgct aattattggt taaccagcaa acaggagtat taaaatatct
    28081 tgttttattg aatagcagga ggaaatcatg aaattccatt atcatatatt gatcaatcca
    28141 tcggcaggca gcggaaatgg ctataaagta gcagaaagaa ttttaccggt attaaaaaat
    28201 aagcatattg actatacact tcattatagt gaatacaaag gacacgatgc tgagattgct
    28261 gaaactttgg caaaagaaac attgattcct tggatagaag aagaacagaa aacaatcgat
    28321 actttccccc ttttgatcat cgtaggaggt gacggcacct tacatcaggt acttgatact
    28381 ttctatcaga tggaagtaga attcccagtt gcttatattc cagcaggttc tggagatgat
    28441 tttgctcgag gggcaggcct tccaaaaaat cccaaaaaag gacttcagct gattttagca
    28501 gctcaatcac cagaaaaagt ccacataatg gcttatgaag aaaaaataag tgaaaaaaaa
    28561 ggaatagctg ttaataattt cggtatagga ttagatgcag ctatcgtcca tgcgacaaat
    28621 cattcttcca caaaaaagcg attgaacaaa tacaacttag gatcattttc gtatttattc
    28681 tctattctcc gtgctttatt cactcaaaaa ggatttccaa ttctggtaga tgcaggagga
    28741 aaacaattaa gtttttcaaa cgcattttta tgtacagcaa cgaagcatcc tttttttggc
    28801 ggaggaatcg cgattgttcc aactgctgat gcgtctaaac cagtcattga ttttgtcgtg
    28861 gtcgaacgaa tcaatttctt taaaatcttg tggctcattc ttttacttat acagaaaaaa
    28921 caattgaaat cgaaatattt ccatcactac acaacaaaca gattacgtat cgtttcaaca
    28981 acaccgcaac atggtcaaga agatggagaa gaaatggaaa aacgaccttt tgatatcaca
    29041 ataaccacaa ctaaacagtt gttttggtgt taataaaaga ggttttgtca gaagctacat
    29101 ctggaacacc gtttattcgg aaaaaaggac tgtgacacga cttttgtcac agtccttttt
    29161 ttactatgca gttaattctt tgcccaattg ggttgccatc gatagtttct ttctttaccc
    29221 aaaataaatt ggatcattga aaacaccgca gcaaaagcaa ctaagacaca accattgaac
    29281 caaaaagatt ctgcaaatgt ttgattgtaa ctgagtgttt cactgatcaa gttattattt
    29341 tgaacaaacc aatatgttgg tgtgaatgcc gctattttgc taaccatttc tggcaagtat
    29401 tctgctggta caaacacccc accgataaag caactgcctg tgataaaaat atctttgatc
    29461 ccaccgctgg tttctgagtt ttttaccaat gaagtgatac aagccgaaaa gctaatgatc
    29521 ggcaataaaa acaagaaagt atttaaaata tgataccata ccgttatatc ccacgaggtg
    29581 tggatcacta acagaaccat tagaagtaag aagccactga acaacagcag cgagtaacta
    29641 atcaaactaa aggttacttt gcgattcaag tttcgtttgg taatgggcgc actctggttt
    29701 cgtttactga tctctttgcg attaaatgct agattgatga cgctgaaacc gctaaaaatg
    29761 gatagaaaca acccataaga caagaaatta aaggttgtgc ccgtcaacag ctttttcatc
    29821 cgctgactat atcccgcact tagctcaact tctccagtaa ccgagagtgc catttgtgtc
    29881 tgttccatag ctgcatcgat tgttttatct tcttcttgaa acagaatcag tgtttgcaaa
    29941 aaggtgttca cttgctgtgt aacaactgtt ttagaaaagg catctgggcg actctgtatc
    30001 ttgatcgtcg gcatctttcc tacttcgact tgttcggtaa agtctgcagg cagatgtaag
    30061 ataatgttta cacgattaaa ataaagagca tcatcgattt ctttttgtga ggttccatcc
    30121 aatgtaacga ctgtttcctt gtcattcaaa tatttacgaa ggccatcgac tacaggcgac
    30181 tcttcttcac taatcaaggc gattttcgca tcttgcagag aggtattttc aacatcaata
    30241 ttttgcgcaa aaaagaatgt ggcaacaaca gtgataatta cacctatcag taaattcgat
    30301 ttatttgctt taagcaatgt ccaaatcgtt ttataaagcg tcataacgca cccctctttc
    30361 catccaagca tttcctaacg caaaaaggaa aataaatccg atcaaccata gaagattggt
    30421 ataaaaacta gccaaatcag tgtagaaata aagtttatac aaactttcgc tgatcaagtt
    30481 gacgagattt aattgaccaa caatcggaat atgaagatca atccaatact tcacttgttc
    30541 cgaccccatc ataccagcaa aaaagctcat cgcaatcgtc actgaaatca agatgctatc
    30601 cttttgcacc agattcattt ttggtatcaa attaccaacc aatgtaccta acaaaatcgc
    30661 acacaacgac cccagagcgg taacaatcat caaccattgc caacgttcac caaagtctac
    30721 ttgatagata aaacgaaaga cagctaacat aatataaatc tctgtcaaaa caatcgtaaa
    30781 gcttgctgcc aaattagcga ttgaaactaa cgattgcttt ctaggaatca aggacaagcg
    30841 aataccattt gacgattgat ttgcttgctg atcctgtaca ttgcggaccc cccacatcgt
    30901 cccgtagaga atcgccattc caatcagagt gaagaaataa aaacttttga aactcatatt
    30961 acgacctgca tgaacttctt gaaaagatac ttcttgttca aaaacagtca tgatgggcgc
    31021 gagatcaacg ttctccactt ctgccacttt actcgaaatt ataccaatac gctgtaaaaa
    31081 ttgatccatg acgtttttca tcaacgtctg actcattcct gcttgaccca catggagcga
    31141 aacagatgaa ccatcaatga gataaaaacc agccaaatca tctgcattta gcttttcatt
    31201 cgcttctact tccgatagtt cctgtacttc aaataatggt ttatcattga cttcgacttg
    31261 ttctaagact gtggtaaaat tttccgaaag cgctgtttga tccgctttga ttacaattcc
    31321 agtattaatc gtttcgagat tgccaccttg atcaatctcg ccaaaggccg ccataaacat
    31381 caatcctaaa agaattggaa aggccaatgt ccaaaacaac atggatttat ttcgcagcag
    31441 tatttttgtg cgatatttat aaaaatgcca aaacattatt tcccctcctc tagtctctca
    31501 aatccttgcc agtaatctct aaaaagacat cattcaagga cgcttctttg gttgctattt
    31561 ttccgtaact gatcttttct tcttgtaaga ttgtaagaat cggcaacaat actgtattta
    31621 gttcagtagt tgttacttgg agctgacctt ttttataagc gattccatca gaagctattg
    31681 ttcgctgaca acacgttttc tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc
    31741 cgtaccatat cttttagaga ttctttggtt cctttagcaa tcacctgtcc ttgatccatg
    31801 atcacgatct ggtcgcaaag aatttctact tcttccatgt aatgtgttgt atagacaatg
    31861 gttgctcctt gtcgattcag ctccttgata ctttctaaaa ttttattccg actttggggg
    31921 tctacagcta ctgtcggctc atctaaaaaa attaaccgcg gcttgtgggc aatcccacat
    31981 gcgatgttca accgacgttt caagccacca ctaagttctc ctggacgaaa cgaaacatat
    32041 ttttctaatc ccactaattg aatcacctct tcaatcaagg ctttcctttt ctctttttcg
    32101 gaaacatata aaccgcaaaa ataatcgata ttttcttgca cacttagttc gtcaaagacc
    32161 gcaacgtctt gaggaacgac cccaatcatt gccttgacag cataattttc aggagtcatt
    32221 gttgtcccga acaatttaat ggttcctttg tcataattta acaaagaaag catacagttg
    32281 atggcagtcg atttcccact gccgtttggt cctaacagac ctaagacttc tccttcttgt
    32341 acttgcagat caaaatgatt caatgctaca aaatctccat agcgtttgac taatttatca
    32401 atttcgataa tcatcatgaa aaactccttc caaatttctt gttgatctga ctataataaa
    32461 gtgcaagaaa agaaactagt gacgattggc taaaccacac atgacatttg tcacaatgaa
    32521 ggagtctgct atgcataaat ctcggctact caacgaaact agctttctac tggttatctc
    32581 attgcttttg ctggtaagtc aaggattcgt tgctatgcaa gttatctatt tattgatcgc
    32641 cgttttattc actggtgtca gttatttact gcctacaaag ccaaattgga ttttccaagt
    32701 tattctattt cttttatgtg cctttttacc tgatcttgtc ctgtttctgc cagcgacgat
    32761 gcgagtgatc gcccctgaag aaagccacct tgattacttg tttttgttag gttttttgtt
    32821 gatttcgacg gttggtaatc atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt
    32881 tttttccttt tacttacgaa aaaaagatca acgaacgacc gatcttgaga aaaagtacat
    32941 tgtctccaaa gatactcatt gggaaaaaca acaagagtta tcggaacaaa ataaagcatt
    33001 ggccctgtca caagaaacac aattagcctt gcaaattgtg gaagaacgga accgaattgc
    33061 ccgagacatc catgacaatg tcggtcatct gctgtcaagt gcgattttac aaattggtgc
    33121 gcttgaaatg atcaatcaag aaccaaaact aaaagaacca ttagaaaatt taagtgccac
    33181 cgttcacact gggatggatc ggattcgtca aagtgtccat gacctccatg agacatcgat
    33241 ctcttttcaa cactccttag agtttctgat cgctgatttt ccttcttctg ttacgattga
    33301 aggaaacttg tttgaaggat tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa
    33361 agaagcctta accaacagta tcaaacatag tcatgccaaa accgttgttt tacattttcg
    33421 aacgttgccg gccttttatc gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa
    33481 ttctgctccc ggtattggtt tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca
    33541 agtccatatt ttccaagaaa accagcaatt tcagttaacg atcattttac caagggaggg
    33601 acaccaatga taacagtagt cattattgag atgatccatt tgtgacgaaa tcgttacaaa
    33661 ccattttaga aagcacgaaa gagatccgtg tgttaggcat tggtcattgt gccaaggatg
    33721 cgctagtctt atacgaaacc catcgtcccg atgttttgct gacggacatc cgcatgccta
    33781 aacaaacggg catcgatgct gccaaagaaa ttttggctaa atttcccaaa gccatccttt
    33841 tgcttttgac gacattcaaa gatgaagaat acatccgcga agcattttcc atcggtgtca
    33901 aagggtattt aatcaaacag aacttgcaag cgattattcc ttctgtcaaa tccgcttata
    33961 atggacaggc cgtatttggt aatgaaatca tcgagacttt cactcaaatg atgaagaata
    34021 aaccgacgat tgatagtcat gcttatgctt cttttagtga acgtgagctt gcaatcatca
    34081 aagaagtagc tgccgggaaa aacaataaag aaattgcaga tgccttgtac ttgagtgacg
    34141 gcactgttcg caattacatc agccaattac tagaaaagct agatttacgg gatcgcacgc
    34201 aattggctat ctatttttat cagcatttgc aataagtctt atctaacaca aaaaaacctg
    34261 aatgatgctc cttcgcatca ttcaggtttt tttcacatct tctattcaat tgtcccgcaa
    34321 accatttcct cacgattgaa agaatcaagg attcgcaggg ctgccaggat tcgttggatt
    34381 gttcggcgtg ttaggcggaa aaataggcgc cttgtctaac gttttttcta gttttgattc
    34441 ttcacgatcg tctcgttcta catcaggact ttcattcaat ttgaattctt tttccatctc
    34501 atctaacttt ttaaagtcat tgccagattc ttttgctaat ttcgctaatt tatcttcggt
    34561 ttctttagcc ataaagtaac gccccccttt tgatttcagc ttagcatatc acactgaaaa
    34621 aacaaaagaa aacgctaacc aacgatcatt tttagctatt tctcatcttt aaaatcaagc
    34681 attttttgcc ggtataaaat cgtagggaga actgttttcc ataccaatca tcggatctac
    34741 taactggtta tcgatcactt ctgctgtaag aacgaaatct ttagaaacag caggttctac
    34801 ctcttctttc ttttcttctc caacaaatga agaaaacaga tctggttcca ctgtttccat
    34861 tacttggttt ttcgtttcat tcgtcccact gtcatcttct acaggtagct tttcttttac
    34921 aacccgtggc tcttttgtat agttcccatc ttcaaatgga tcatttccga ccaaagaatc
    34981 ttcataagct gctagtttgg tccggagagt gatattcatt ttatcattat ccatgatcct
    35041 tgatttcaac gtagtcatcc ggcttgctga ttcattcgct acacaagtga cataggcttg
    35101 ctcttcccct aacaaaatca acagttcctt actgcgagtc actgccgtgt agagcaaatt
    35161 acgctgaagc atccgctgat attgatggac catgggcaaa ataaccatct tgaattcact
    35221 gccttgtgat ttatggattg agcaacaata agaaagagtg attttattcc attcgtttct
    35281 tttataagtg acctcatttg catcaaactg gataactaat tcatccactt tatcttcaga
    35341 atcttttgcc aagatgatac ccgtgatttc tcccatatct ccattgaata cattcaattc
    35401 gggagtattg actaattgga ggactttgtc tcctatccga taaacggtgt cgttccaatg
    35461 tacttccttt tttccaggtt tccctggatt gaatatctcc tgcatcattt tatttaacgc
    35521 atcgatccct gcaggtcccc gatacattgg tgctaatact tggatatctt gtgcacggaa
    35581 tcccttattt ttcgcttttt ccacaatttt agagatcaag ggttcaattt gataagcatc
    35641 acttcggaaa taggaacgat ctttttgatt ctttgtgaaa tcttttggca gacgtccttc
    35701 tttgatctca tgggcaagag gaataatact agaaccatca ccttgccgat aaatctgatt
    35761 gagttccatt ttgggaattt gttcgatttc caaaagatca tggagtactt gaccaggacc
    35821 gacagaggga agctgatctt tgtctcctac taagataact tgcatattcg tcggaatcgc
    35881 tttgaataat gtgttagcca gccatgtatc gaccatagac atctcatcaa caatcaaaag
    35941 tccgccttct aattctttcg tactgactga aggtgttttt tcccgtccat tcagccccag
    36001 aagacgatgg atcgtactgg caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg
    36061 gccagttggt gctgcaagta gaataggaaa aatttcttgg gtatattttg aaggatccaa
    36121 ttccagtccg ttcaactctg caaaaagatt gacgattccg ttgatcacag tagttttccc
    36181 cgtacctggt cctcctgtta aaataaatag aggggaacgg attgcttcag cgatagcttg
    36241 ttcttgcgaa ggtccatact gtatgccgaa tattttttcg atacgccgaa tattcttctg
    36301 cacttctttt tcaggataat ggatttcttt tttacgactg agaaggcgct ggatcgaatt
    36361 accgatcccc cattcagaaa agtacaaact gttttcgtaa agattagtct cttcttgttg
    36421 tatctttcct tcttcaacaa gttggataat tacagtagca acctgatcag gctggatttc
    36481 aattgggcga cttgtttcta aaatacggat cgtttgctga agcaatgtct ccgccggtac
    36541 gtatgtatct cctgtttgaa ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat
    36601 tcttctcggt gaatctgctg caataccgat ttgttcggct agattatctg cacgtttgaa
    36661 cccgattcct tctatgtctt cgactagctg gtacggattt tcttcgatga tatccaaaga
    36721 ctcgttctta taggcctggt agatactgaa agctaactga ctaccgaacc cgtaacgatt
    36781 cagtcccatg atcacttgat ccatcccgtg gtttagccga atcgtctcag caagcatttc
    36841 tctttttggc ttcgtcaaac cggagatttg ttttaatacc gaaggatcag caatgatttt
    36901 ttcgatagca tcttctccca gcaattctac aattttttct gctgttttct tcccgattcc
    36961 tgggaatttt tcgctggaaa gatacgctac gatcccattt gcagttgttg gcttttcttg
    37021 ctcatatctt tcgagtttaa gctgccttcc gtatttagga tgatccacta attctccata
    37081 aaaacgataa gtctcatttt cttgaatatc gccgaaactt ccggtgacta caatctcttt
    37141 ttcacggtaa tcagctgtgg tttcactgat gtgtaccaaa agcactttgt aaaaattact
    37201 aggattttga aaaaaaatcg cggcaactgt accaacgaaa taaggcattt cttctggctg
    37261 catcggaagc cgcctccttc ttctatagta aaggttctgg tgctgtttca gttcctaaaa
    37321 aatctgtttg gttccatact ttcagttcat atggcaataa tctgcaaggc tcttcagtct
    37381 ctaaaatcgt caaactacta ttgaacaatc cacccatttc acgcaactca cttagctctt
    37441 ttcctgtcat ccattgaatg gctgcagtta aagaagctcc atgtcctacg aataaaagtg
    37501 ggccatcttt atgttgggca accgcatctg ctacaacagt ttcgatacgt gtgattgccg
    37561 attcaatcgt ttctccgtga aaaggagaag gatcatacag atctaaatga ttacgcaaat
    37621 tcggcagatt atccggatat ttctggtaca tttcatcgat cgactgacct tctaaagtac
    37681 ccagccctag ttctcttagc ttatctgtat aaaaaatctc taccggacga gcaagctctt
    37741 tgttgatacc ttctgctgta tcacgtgctc gctttgctgt actggaataa attgctttaa
    37801 aaggaacata ttttaccttt ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa
    37861 gcggagaatc gccattgctc ccttgaaaac gtctttcctg attccattgt gtcttcccat
    37921 gtcgggtaaa atatagttcc atacgcagca aactcctgtc tttcttttat tttactctat
    37981 aataaactgt ctaatccagc ttgtcatttt catagtgatg cgtcaattct aaattaggga
    38041 atccttgctg gcgaagtgct tcatacacta tcattgcagc tgtattcgat aaattcaatg
    38101 agcggacatg ctcatcattc atagggatgc gtaaacactt ttcttcgttt tcacgcataa
    38161 atgtttctgg caaaccggtt gtttcttttc caaataaaaa gaagtgctct tctccatcag
    38221 caaaattttc gtcactatat actttatggg caaattttga gatcaaatgt agtttacgat
    38281 tacctaaaaa tgttaaaaaa gcttctaaat tactgtggta agtaatattt acatcattcc
    38341 aataatccaa ccctgcccgt tttagatgtt tgtcatcagt tgaaaaacct agtggctcga
    38401 ttaaatgaag cggagtattc gtggctgcac acgtacgggc aatatttcca gtgtttgcag
    38461 ggattaatgg ttcaaataaa acgatatgat tcatcaggat cattcctctc atatttatgt
    38521 tcgtttctat tttctgacaa tttgattttt gcataaaaaa aaacgtatca actcggtgtc
    38581 agtcactgcg agtcgttacg ttagtgaagc tcttctcact aacttttatc agaacaggaa
    38641 cctgataaaa accaatgaaa aaacaacttg ttgctaatat aacaccctca actttttttt
    38701 gcaacctcta ctcattaaaa actgctagaa aaacgttttc tcttagttcc ttttaataat
    38761 tctatgtcaa ttgtaatctt cttcaaagga ttcttatata agtctgcttt gattcgctct
    38821 tctacctgcc attctaaagg tgacatggcc aaaagatctt tttgattttc ctctggtatc
    38881 tcaaattcat aggttacacg ctgacgttca gttaatttca ttgcttcagc aaacttggct
    38941 actacacgtt cattggaata actctgtttt tcaggttgat tcggataaaa agcctgacgt
    39001 agttccttta aataattttc ttgtggcacc accttcaaca cttctccatc ttctgagagt
    39061 acacgttgga attcttggta atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt
    39121 tgatcagcaa acggcaaatt ggtcaaatca gctacacacc aaaatgcttc ggcagattgt
    39181 tctgtagctg cgtagactcc ttcttttgaa atatcaaatc caacagcatt ttgaatcttt
    39241 ttcaattttg aaagttcatt caagaagcta ccttctccgc agccaacatc taatagcgag
    39301 acttcttcag gtaaatgctg cgcaatagtc tcaataacgg gctgatacat gccagattgg
    39361 atcattctac gccttggaac gaacattcct tgatcgtatt ctgttttaac cgcatgggat
    39421 aaaaaataaa gtgttccttt tttggaaagg tcaaaacggt gattattttt gcatatcaag
    39481 ccattatcag acttttttac ttcttcttgg caaaccggac agcggaacag ctgatgattt
    39541 tctgccaaaa actggcgtcc tcggtcaatt ttttttaaca tagttccccc tcatttctgt
    39601 tttatcttac cgttagatgc tcactttggc aactgctaaa aatatgatac aatgcctaga
    39661 agaaagtgaa agataaagga gaagattaga tgatcaaaga attttgcgct gaaaactaca
    39721 catctattcc tctagcaatt gctagaggag ccgatcggat cgaattgtgt gacaacttag
    39781 cggtcggtgg cacaaccccg agtactggtg tgatcgaaga aacgttaagt tatgctgggg
    39841 aaaaagatat ccccgtaatg acgattattc gtcctagagg gggcgacttc gtctataacg
    39901 atatcgaatt gaaaatcatg catactgatt tgattgaagc aaagaaatta gggacggatg
    39961 gcgtagtatt tggttgtctt actccttcag gctggctaga cgaagaggcc ttagaactat
    40021 tgatcgaaac agcagaaggg ttgcagatca ccttccatat ggcttttgac agtattcctg
    40081 aagatcggca attcgaagca atcgattggc tggtagaaca tggtgttgac cgtatcttga
    40141 ctcatggcgg tcctgctgac aaaactattg tagaaaattt cccacggctt aaagaattga
    40201 ttgcctatac agatggccgc ttaatcattt tgcctggtgg tggtgtcact gcagagaatg
    40261 cagcacatgt agctaaagaa ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc
    40321 ttcagttata atcttataaa caaaaaaaca tttgcaacag gcataatgct tattgcaaat
    40381 gtttttgttt ttttacataa aatagcccat aaataaagaa gcaatattga aataaatcag
    40441 tacactcgtc aaatcgctca aagtcgtgat aaagggaccg cttgctactg ctggatcaaa
    40501 tcctagacga tccattagca tcgggattaa actacctgca agattcgcca ctgtgattgc
    40561 aaatagcata gccatcccta tgacaaaccc caagataaaa ttatgtttcc aaaaacctac
    40621 gattagaaaa atcgtgatcc ccgtaacagc tcctgtgaca acgccggtca agacttcagc
    40681 caaaacaagt cgaacaaatc catgatcttt ctcatcagaa atcgcaagtc gacgaacagc
    40741 cacagctagc gactgggttc cagcattacc ggcagttcct gtaatcagcg aaatgaagac
    40801 tgctaaaata ctcgcttcac ttaccaagtc ttcatagtga ctgattaaag aagcggtcga
    40861 catacctaaa aacaacagag tgatcagcca aggcaaacgt cgagacgctg cctttactgg
    40921 attttcatta atttcttcca cattgacccc ggcaagtcct gaataatcgc tagcagcttc
    40981 atcatcaata acgtcgatga tatcatcgac tgtgacgatt cctaacaaat gatcatcata
    41041 atcggtaaca ggaagagcta agaagtcata atctctaaat gtttgtgcca catcttcttg
    41101 gtcgtcgcct acatgaactg aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc
    41161 gtcatcattc acgattaggt cgcgcagaga aataacccct actagctgac cttcttgatt
    41221 aacgacatag atgtaataga ttgtttcagc tacatctgct tcgttcttca gcacgtacat
    41281 cgcagagcga acagtttgat tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc
    41341 agccgtttca tcctcgtaat ggagaagttc cttgatttca ctggcatcgt ctgccgacat
    41401 caaacttaaa tatttagcaa tttgcttttt atccagcgta ttaagcaagt caacagcatt
    41461 atctgtatac atctcagaaa gcatatcagc tgcatagcta ggacgcattt catctaggta
    41521 ttctttcata tgttcgttgt cttcttcaat cacatcgaac atatccgcta attctttagg
    41581 cgacagatag gaatagatca gctgcctgtt ctcttctgta agagattggt agaattgccc
    41641 ctgctcatag atatgcatct ctaaaaaact gtttctgaat gcctgaatat tattttccct
    41701 taaatcttga gtcaactggg caaatctttc ctctaattct tgattttcat ccaaaccaat
    41761 ctcacctcgc cttattcttc aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc
    41821 tgcttgaact ccagcaactc ttctgtaaat ggatggtaaa aacgcagatc ataacaatga
    41881 agggcttgtc tttttattcc cagatccatt cgcccgccat acatatcatc accgagcagg
    41941 gcacagccaa ttgcggaaaa atgaactctt atttgatgtg tccttcctgt atgcagctgg
    42001 atatcaacta acgcgatttc tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa
    42061 cgtccttcat cagagaccat tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa
    42121 tcgatatgtc catgtgccga taaatctgaa agccttccac tgactaaggc ctgatatttt
    42181 ttcacaaact tcttttcacg aagttgcata tctaattttg cgtgcgcaaa tccatgcttt
    42241 gcaaaaagca tcaaaccaga tgtatcccga tctagtcgcg tcaccacatg gattacttgg
    42301 ttttcgtaac cttgtttttt ataataagcc tttacacgat tagccattgt cccatttgga
    42361 tggtattggg caggaataga cgaaacgcct gctggtttat ttactaccag aacgtgttca
    42421 tcttcataca cgatatcgat cggtgtttca tccaaaagta cggtttcatg ttctccttca
    42481 gcaggaatca caatggttac cttatcatcc ttagctagag aaaacaagac attttctact
    42541 tgatcattaa ctttgatttg accgccttga aattttattt tcgctaacag accctttgag
    42601 atcccttttt cttttaaaaa atattttact tgctgagaat gatctttctc ataatcccat
    42661 gtaaactgca tatcaatcct cactaataaa ggcatctttc acacgatgcc aaaaatgcat
    42721 atgtcgataa gaagcaaaat ggatccgttc ttctgctatt cgataataaa ttgagcggat
    42781 atttgctttt gcgacattca attgatcaac cgttacaaga taatcttctg tattttccaa
    42841 ttttatttct agccattcat catgggcaat cacgatcgga gaacccaaag tacgaaacac
    42901 tcgattattc agtgaagcaa tctctgccaa ctgaaaggca ttgatactag gatggatcac
    42961 agcaccgccg acacttttat tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc
    43021 atctcctcta aaactttcaa acagctcgtt tttgatgtat acatcggcta ccatcgtacg
    43081 attggcccgt ttaatcgttg attcattcag tgctaaaaaa tgctggtccg gtgtttcatc
    43141 caaataactg atccgaacat ccagcaaggg ataactaaca ctttgttccc ggttcgttcg
    43201 taagctgtct actagttctt ccagctcgta atcccgccaa tccgtgtaaa accctaaatg
    43261 acctgtatgc acgcccaaga aacgcacatc attcagacaa tgactgtagt gatggaacgc
    43321 cgacaataat gtgccgtctc cgccgactga gatgaccaat tctggctcat gatcatctcg
    43381 ttgaatccct gcttgttcta aaagtgcgtt caaacgttct gtcacttcta tagtctttgc
    43441 ttctttatta tgaacgatcg ctactttcat cccctgcctc ctctctttcg ctttctttct
    43501 tcaaaagcgt tcgatagtag acatcatctg attttcctct tccatgggag aataaccgct
    43561 gtgcttcttg gatttcttca cggatactgg acatctcttc atccaactga taggcagctt
    43621 ctgccgcacg ctgaagtcgt tcactcattt cttctggaaa aacgccttga tatttataat
    43681 tcagcgaatg ctcaatcgtt gcccagaaat tcatcgcaag agtccgaatc tggatttccg
    43741 ccaaaatttt cttctcaccg ctgatcagct gtacaggata ctcgatcacc agatgataag
    43801 aacggtagcc acttgctttt ttatttgtga tgtaatctcg ttcttggaga actttcatgt
    43861 cttttctttt acggatgatc tcaacaactt gatggatatc ttctacaaac tgacacatga
    43921 tccttaaccc ggcaatatca gacatttctt cttctaaacg tcccattgga atatgccgta
    43981 attgtgattt tgttacaata ctatcgactg gcttgactct cccagtgaca aattcaatcg
    44041 ggacatgtcg attttgttca cggaactgtt tgcggatccc ccgcagtttc actttcaatt
    44101 ctgatacagt ttgttcatat ggtgctaaaa atgcttccca atctcgttcc atagctgata
    44161 cctctacttc attaacattc attgtttatt ctaccatata tgcaaagttt tttcgtactc
    44221 ttgatttatg cattgaccaa aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc
    44281 aaatgacaca aatgcttgaa atcgaatata aatctatgct gacaaagcaa gagtacgaga
    44341 tattcatcgc tcactatcaa ttaacagata aagatttccg tgtacagact aatatttatt
    44401 tcgataccgt tgatgaacag ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg
    44461 gtcatcaagc tgaatataca ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg
    44521 atacctttat aacagaagaa gcagaagcat ttatcttcga aaaacgtctg cctcgttcag
    44581 gcgcagtttt tcaaaaactt tctgatttga atatcgatcc tgcttcatta atccaaaccg
    44641 gaaagcttac aacaaaacgg gctgaattcc ctattgaaga aggactttta gctattgatg
    44701 aaagctgggg agacaatcta cacgattttg aactggaact agaggtaagg ggatgcatct
    44761 gtgggaaaaa ttgcttttat aaatttttta aaacgttttt ctttacctta tcgtcctgct
    44821 aagaacaaga tccaacgtat gcttgaagct aaaaattaga aacctttcat tttcacaagg
    44881 cacttgagtt cattttttta cttttttctg ttaagttata ggatgtgaaa gaatctagtc
    44941 actagttgta tagaatctat tttgggggag attcatatga tcgaaatcta tttatttgtt
    45001 aatcctttag gggaatactg tttcgaaatg gaacaacagt tattacaatt tatcgaagaa
    45061 gagtacggga caccatcaaa agaaaagatg caatttcgct ttcttccttt ggtcaatttg
    45121 cagacaattg gggatgtcat gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt
    45181 aatcacttat tctcaatgac ttattctgca gcattggact gcaaagcggc gcagtttcaa
    45241 ggaaagaaaa aaggccgccg ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac
    45301 ataccatatt ctcaagaact tgctgaatcc atatttgaag aaatcggcgg cgatttgaat
    45361 atgtttaaag aagatcgtca gtctgacttc gttaaagaag tattcttttc tgatcaaaaa
    45421 attgctcggg aaatgggcat cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc
    45481 gaccgtgatt ttggtgtttt agtagaagta aaaggtacaa gtagcctttg gagaatcaca
    45541 gagctgtgta aaacaacaga taactcatat caagttttta atggtgaaca tttacataca
    45601 aatgattctc agttccattc aagaactagc catttacact tgctttctaa ttaaaaaaat
    45661 acgtcgactc ccttacaaga gagccgacgt atttttttcg tttctattcg agaaaatcaa
    45721 aaatagaaag ccttataaac gatcgatcaa ttcttctaat tcatttagac gttgttcaaa
    45781 catcgacatt gcatcttcaa tatatgcagc ctgtgtcata tcaactcctg cttttttcat
    45841 tacttccaca ggataatcgc tgttacctgc ttttaggtaa gccaagtagt tctctagtgc
    45901 ctctggttcc tggtttagta ttttcttcgc cagtgctgaa gcagcagaaa agccagtcga
    45961 gtattggaaa acatagtaat tataataaaa atgcggtatc cgtgaccatt cgaatttaat
    46021 ttctgggtct tcttcgactg ctggaccata atattttgca ttcaatttac cataactatc
    46081 acttaggtat tcactagtca atggcacacc tttttcatct tccgtatgca tgaaatgctc
    46141 gaattcagcg aattgtgtct ggcggaaaac tgttccttta aacccatcca agtaatggtt
    46201 gagtacataa gcccgtacac gagggtcttt ttctgtttcc aataaatact ccgttaggat
    46261 attttcattc gttgtcgaag caatttctgc taaaaagatg gagtagtcgc cgtacacata
    46321 aggctggttc gaacgagtga aataactatg aacactatgt cccatttcgt ggacaagcgt
    46381 aaatagctga tccagcgtat catgccaatt caataaaata tatggatttg tgtcatagct
    46441 tcccgaagaa taagcaccgc ttcgtttccc tttattttcg acaacatcga tccaacgttc
    46501 agagaatgct ttttctacga tggccatgta ttcttcaccc attggtttca gtgcttctaa
    46561 agctttttct tttgcttctt cgtacgtaaa ggtaattgga gcttccccta agaccggtgt
    46621 ataaagatca tacatgtgca gtttttccac ttctaataaa cgtttccgta attccatgta
    46681 tcgatgtaac aaaggcaaat gcttgtttac cacgtctacc aaagtatcgt atacactttc
    46741 aggaatatga ttattgctca aagacgcttc tctggcagag ctgtaattac ggactttcgc
    46801 tttaaaatta tgtcctttta tatgtgtgcc taaagtagaa gcaaatgtat ttctaaattg
    46861 ttcgtaaaca ctgtacaatc ccttaaatgc tgcttcgcgc acccttcgat ccgtgctttc
    46921 tagcaactga ccatacacgc catgagataa ttggactatt tcaccatttt ctccttcaat
    46981 cgttggaaaa actagatctg cattattcaa aacagcaaat gtatcacttg aagcatcaaa
    47041 gatttcacct gctccagcaa gaagagattc ctgctcagca gataagacat gggctcgatt
    47101 atctactatt tgctggatat aatggcgata gacttccaat tttggttctt ctttgaaata
    47161 ttgccaaatc tggtcatctg acaattgcaa tatttctggt tcaaaccagg aaacggcttc
    47221 actaactttc gaaaacagac tgcttgctct tgcataaagc gcttggtaat ctgtatttcc
    47281 agtgtcttga tcgtttttaa ggtgcgcata tacataaatg acttcagttt ggcgataaac
    47341 cctcaatacg aattcaatcg catttaaaaa ttgagaagcg ccttgatcaa gtgttccttt
    47401 gtgtttttca gattgtttta actcttctga taattccaaa tatttctcat caaactcttg
    47461 gtcgcttgaa aagatcttgg tcaagtccca agttaaattt tcaggcaatt cttctcgttt
    47521 tggcaactgc tttacttcca tgaaatccac tccttttctt cccttagctc tatctatcaa
    47581 aatagatcat agtctaacat ccacatgtca cagcatctaa cttttctact cattcatgtg
    47641 tctgcttcca tcaattttag cataactaaa tcttgtttta catagaaaac cgattcctag
    47701 cctattttta tacttactat tttttcaaag aagacaagtg tatgcattcg ttaaaaaact
    47761 gctgatagac catctcttca tcaatcatag gaaaaagcca ttccctttta tgttctttta
    47821 tttggcaaag ccatgagttg aaatcaggaa ctttctggtt ttccaaggct tcttcgtaca
    47881 acattcgata aagaaaaacc tgttcttgta aataaaagaa aaaccgactt ttttgataga
    47941 tccacgatga tagatgcaaa agatgtttat gacgcaagta gcattgttct tgtatggata
    48001 ctgtattctt ttgcttgtga ataagttggc gtgtcagcca tttcttccca tcttcactca
    48061 ctttttctat ttttttcatt tggttcattt taatttcgtt actagtgaat atttctttaa
    48121 ggtccctact agaaaaagtc cagcagctac tgcaaaagga aatatgccct gatacagtct
    48181 ctgtaatatg atgatacaaa taaagctttt gtttcttgag atcagctttc cagcaatgca
    48241 ctcctctttt tctattatag tagcagcaac ttttttctat caatgagaat tgcgattgtc
    48301 caagaaaaga attaccaagt atccaccata cttggtaact gttattcaaa taattttggg
    48361 ttctttcaat aaatctctgg tgagacagtc tagaacattg gatttctatt gctagattcg
    48421 ccaacaaaat atccggcctt tgccgcaacc tcggcaaata ggcttccaaa aatactggtt
    48481 ctactgactg tcccagccaa tccatgaagc attcctttaa ctgtaagtgc tcttttgatt
    48541 ctctttcact aaagaccgaa cagttatctg atttttgatg ggcaaaatga gcacattttt
    48601 tctttccctt tttcaaaatc actctttttc gacagccacg acaataatat ttctttcctt
    48661 tttctgcatg tttagcaaaa cagcacttat tatattcatt cattgcaagc agcatttttc
    48721 tccctctgaa cttatttacg tctaagaatt tatttacacg aaaaaagagg gcgtgaaaaa
    48781 gctattctgc atcaagtaat aagaacagct aaacaaaaat agttcccaat atttttcgtt
    48841 tggttgggct tgttaccgca gatgcaagct tttgaacaca tttatacgaa aaaaagactg
    48901 cgacacaatt tttgtcacag tctcttacta taatgctgat ttatacaaaa taaaatttcg
    48961 ttcgctcgat agcattacgc gccatcagac attgtccata ttcattcaat gtttctggtg
    49021 tacgatcgct cttttcagca aactccaaaa ttcgagcaat ctgattctct acatccgctt
    49081 ggttgagatt ttccatccag aagtgaacga ctaggtaata gcgctcttcg aataaataca
    49141 aatcagtcca agcagactct agttcgacat ctgttgcaag ttggatcact tgttcaaaat
    49201 cttcaaaagc aaaagttgct tgtctttcct gttcttctag ggcgcttcca gataattctt
    49261 cctccggatc gctgccaata atctgctgac gaagaaagtc actcacatct tctgaatcaa
    49321 cagtagaatc ttctgattga gaaatttctt catccggaag atttttactg ataaacagtt
    49381 ctaatccgtc tccttttggc aaaacttgga aagtgacagc ttcgcttcct cggaactcct
    49441 cttcgatatc aacttcttcc aaaatactat aaaagaaatt ttcgatttct ttgtgattgc
    49501 caagtaaatc caaaaatgaa acgccccgtt cctctaaatc ttcatgacca attagtacac
    49561 ggatcgtatt ttcattaata tgttccattt ccattttgct acacctcact ttgccacaat
    49621 gtttaatata gattcattgt agcgaaatca gcttaaatgt aaagaaaaaa aatctcgctg
    49681 ttaagcgaat ttaataaaac cagcggattt ttcaaccttt caagctatct tatttaacta
    49741 taacattttt ggcagacgaa tactatatag ccgacttttc agctaatttt ccctctctat
    49801 ttttcgtgca atcatcatct tctactacct ttttatacat ctaatcaaga atactttttg
    49861 taaaaacaaa aaaacttgga aaaactatct ttgtaatagt cttttggctc tatacttttt
    49921 gatgttggtg aacttcacca acatcttttt tgta
//
LOCUS       DASJTM010000008        48399 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJTM010000008 DASJTM010000000
VERSION     DASJTM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.008"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..366
                     /locus_tag="U4B29_000424"
     CDS             172..366
                     /locus_tag="U4B29_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HEN2105394.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(531..737)
                     /locus_tag="U4B29_000425"
     CDS             complement(531..737)
                     /locus_tag="U4B29_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105395.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            1023..1433
                     /locus_tag="U4B29_000426"
     CDS             1023..1433
                     /locus_tag="U4B29_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HEN2105396.1"
                     /translation="MAQVAIFKEIFDQVRKDLDCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1723..2016
                     /gene="bor"
                     /locus_tag="U4B29_000427"
     CDS             1723..2016
                     /gene="bor"
                     /locus_tag="U4B29_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HEN2105397.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2048..2509)
                     /gene="rzpD"
                     /locus_tag="U4B29_000428"
     CDS             complement(2048..2509)
                     /gene="rzpD"
                     /locus_tag="U4B29_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="HEN2105398.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNAREL
                     KLANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSV
                     REATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(2506..2982)
                     /locus_tag="U4B29_000429"
     CDS             complement(2506..2982)
                     /locus_tag="U4B29_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEN2105399.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2966..3118)
                     /locus_tag="U4B29_000430"
     CDS             complement(2966..3118)
                     /locus_tag="U4B29_000430"
                     /inference="COORDINATES: protein motif:HMM:NF016962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2105400.1"
                     /translation="MRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGV
                     EDGRNQ"
     gene            complement(3122..3289)
                     /locus_tag="U4B29_000431"
     CDS             complement(3122..3289)
                     /locus_tag="U4B29_000431"
                     /inference="COORDINATES: protein motif:HMM:TIGR01594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HEN2105401.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIA"
     gene            complement(3966..4589)
                     /locus_tag="U4B29_000432"
     CDS             complement(3966..4589)
                     /locus_tag="U4B29_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HEN2105402.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(4586..5251)
                     /locus_tag="U4B29_000433"
     CDS             complement(4586..5251)
                     /locus_tag="U4B29_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN2105403.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMNKLDTIGFDNKKDLL
                     ISVGDLVDRGAENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKDKKYVICHADYPFDEYEFGKPVDHQQVIW
                     NRERISNSQNGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(5229..5435)
                     /locus_tag="U4B29_000434"
     CDS             complement(5229..5435)
                     /locus_tag="U4B29_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HEN2105404.1"
                     /translation="MTFSVKTIPDMLVETYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(5432..6046)
                     /locus_tag="U4B29_000435"
     CDS             complement(5432..6046)
                     /locus_tag="U4B29_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HEN2105405.1"
                     /translation="MMAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSK
                     EREKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPC
                     ISCGTLTSAQWDAGHYRTTAAAPQLRFNERNIHKQCVVCNQHKSGNLVPYRVELISRI
                     GQEAVDEIESNHNRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(6036..6206)
                     /locus_tag="U4B29_000436"
     CDS             complement(6036..6206)
                     /locus_tag="U4B29_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HEN2105406.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     PNSSMHEEEDDG"
     gene            complement(6203..6385)
                     /locus_tag="U4B29_000437"
     CDS             complement(6203..6385)
                     /locus_tag="U4B29_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HEN2105407.1"
                     /translation="MARQRRSITDIICENCKYLPTKRTRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRRRARKTR"
     gene            complement(6352..6525)
                     /locus_tag="U4B29_000438"
     CDS             complement(6352..6525)
                     /locus_tag="U4B29_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HEN2105408.1"
                     /translation="MMRCYRCGECKEDNRFRPNQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            complement(6522..7394)
                     /locus_tag="U4B29_000439"
     CDS             complement(6522..7394)
                     /locus_tag="U4B29_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEN2105409.1"
                     /translation="MINVVSFSGGRTSAYLLWLMEQKRRAGKDVHYVFMDTGCEHPMT
                     YRFVREVVKFWDIPLTVLQVDINPELGQPNGYTVWEPKDIQTRMPVLKPFIDMVKKYG
                     TPYVGGAFCTDRLKLVPFTKYCDDHFGRGNYTTWIGIRADEPKRLKPKPGIRYLAELS
                     DFEKEDILAWWKQQPFDLQIPEHLGNCIFCIKKSTQKIGLACKDEEGLQRVFNEVITG
                     SHVRDGHRETPKEIMYRGRMSLDGIAKMYSENDYQALYQDMVRAKRFDTGSCSESCEI
                     FGGQLDFDFGREAA"
     gene            complement(7391..7831)
                     /locus_tag="U4B29_000440"
     CDS             complement(7391..7831)
                     /locus_tag="U4B29_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HEN2105410.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(7905..8195)
                     /locus_tag="U4B29_000441"
     CDS             complement(7905..8195)
                     /locus_tag="U4B29_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HEN2105411.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLVFKECRQSAAMKRVLAVYGVKR"
     gene            complement(8192..8893)
                     /locus_tag="U4B29_000442"
     CDS             complement(8192..8893)
                     /locus_tag="U4B29_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HEN2105412.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(8890..9789)
                     /locus_tag="U4B29_000443"
     CDS             complement(8890..9789)
                     /locus_tag="U4B29_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2105413.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(9822..10115)
                     /locus_tag="U4B29_000444"
     CDS             complement(9822..10115)
                     /locus_tag="U4B29_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HEN2105414.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            complement(10234..10434)
                     /locus_tag="U4B29_000445"
     CDS             complement(10234..10434)
                     /locus_tag="U4B29_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2105415.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            10535..11248
                     /locus_tag="U4B29_000446"
     CDS             10535..11248
                     /locus_tag="U4B29_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEN2105416.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            11361..12200
                     /locus_tag="U4B29_000447"
     CDS             11361..12200
                     /locus_tag="U4B29_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rexA"
                     /protein_id="HEN2105417.1"
                     /translation="MKNGFYATYRSKNKGKDKRSINLSVFLNSLLADNHHLQVGSNYL
                     YIHKIDGKTFLFTKTNDKSLVQKINRSKASVEDIKNSLADDESLGFPSFLFVEGDTIG
                     FARTVFGPTTSDLTDFLIGKGMSLSSGERVQIEPLMRGTTKDDVMHMHFIGRTTVKVE
                     AKLPVFGDILKVLGATDIEGELFDSLDIVIKPKFKRDIKKVAKDIIFNPSPQFSDISL
                     RAKDEAGDILTEHYLSEKGHLSAPLNKVTNAEIAEEMAYCYARMKSDILECFKRQVGK
                     VKD"
     gene            12216..12650
                     /locus_tag="U4B29_000448"
     CDS             12216..12650
                     /locus_tag="U4B29_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HEN2105418.1"
                     /translation="MRNRIMPGVYIVIIPYVIVSICYLLFRHYIPGVSFSAHRDGLGA
                     TLSSYAGTMIAILIAALTFLIGSRTRRLAKIREYGYMTSVVIVYALSFVELGALFFCG
                     LLLLSSISGYMIPTIAIGIASASFIHICILVFQLYNLTREQE"
     gene            13115..13438
                     /locus_tag="U4B29_000449"
     CDS             13115..13438
                     /locus_tag="U4B29_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HEN2105419.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHKQIESNLQRIERKNQRTWYSKPGERGITCSGRQKIKGK
                     SIPLI"
     gene            complement(13439..13921)
                     /locus_tag="U4B29_000450"
     CDS             complement(13439..13921)
                     /locus_tag="U4B29_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006641566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HEN2105420.1"
                     /translation="MYMIVIWVGLLLLSPDNWPEYVNERIGIPHVWHVFVFALAFSLA
                     INVHRLSAIASARYKRFKLRKRIKMQNDKVRSVIQNLTEEQSMVLCAALNEGRKYVVT
                     SKQFPYISELIELGVLNKTFSRWNGKHILFPIEDIYWTELVASYDPYNIEIKPRPISK
                     "
     gene            14188..14388
                     /locus_tag="U4B29_000451"
     CDS             14188..14388
                     /locus_tag="U4B29_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HEN2105421.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELGKQNNKAA"
     gene            14571..14939
                     /locus_tag="U4B29_000452"
     CDS             14571..14939
                     /locus_tag="U4B29_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HEN2105422.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            15012..15176
                     /locus_tag="U4B29_000453"
     CDS             15012..15176
                     /locus_tag="U4B29_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HEN2105423.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            15145..15288
                     /gene="kil"
                     /locus_tag="U4B29_000454"
     CDS             15145..15288
                     /gene="kil"
                     /locus_tag="U4B29_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HEN2105424.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            15363..15659
                     /locus_tag="U4B29_000455"
     CDS             15363..15659
                     /locus_tag="U4B29_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HEN2105425.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKSITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            15665..16450
                     /gene="bet"
                     /locus_tag="U4B29_000456"
     CDS             15665..16450
                     /gene="bet"
                     /locus_tag="U4B29_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEN2105426.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     gene            16447..17127
                     /locus_tag="U4B29_000457"
     CDS             16447..17127
                     /locus_tag="U4B29_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN2105427.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            17124..17306
                     /locus_tag="U4B29_000458"
     CDS             17124..17306
                     /locus_tag="U4B29_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HEN2105428.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            17279..17470
                     /locus_tag="U4B29_000459"
     CDS             17279..17470
                     /locus_tag="U4B29_000459"
                     /inference="COORDINATES: protein motif:HMM:NF018795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HEN2105429.1"
                     /translation="MHKASSVELRTSIEMAHSLAQIGIRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERNQV"
     gene            17451..17636
                     /locus_tag="U4B29_000460"
     CDS             17451..17636
                     /locus_tag="U4B29_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN2105430.1"
                     /translation="MRETRYDNHGMHFSGSGLHILCAYACRHGTCSMTPQQENALRSI
                     ARQANSEIKKSQTAVSG"
     gene            17862..18083
                     /locus_tag="U4B29_000461"
     CDS             17862..18083
                     /locus_tag="U4B29_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEN2105431.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            18080..18628
                     /locus_tag="U4B29_000462"
     CDS             18080..18628
                     /locus_tag="U4B29_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HEN2105432.1"
                     /translation="MSEINSQALREAAEQAMHDDWGFDADLFHELVTPSIVLELLDER
                     ERNQQYIKRRDQENEDIALTVGKLRVELETAKSKLNEQREYYEGVISDGSKRIAKLES
                     NEVREDGNQFLVVRHPGKTPVIKHCTGDLEEFLRQLIEQDPLVTIDIITHRYYGVGGQ
                     WVQDAGEYLHMMSDAGIRIKGE"
     gene            18820..19101
                     /locus_tag="U4B29_000463"
     CDS             18820..19101
                     /locus_tag="U4B29_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105433.1"
                     /translation="MSINELESEQKDWALSMLCRSGVLSPCRHHEGVYVDEGIDIESA
                     YKYSMKVYKSNEDKSPFCNVREMTDTVQNYYHEYGGNDTCPLCTKHIDD"
     gene            19190..19357
                     /locus_tag="U4B29_000464"
     CDS             19190..19357
                     /locus_tag="U4B29_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105434.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            19397..19615
                     /locus_tag="U4B29_000465"
     CDS             19397..19615
                     /locus_tag="U4B29_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2105435.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGGLLKRIRNGKKAKS"
     gene            19593..20663
                     /locus_tag="U4B29_000466"
     CDS             19593..20663
                     /locus_tag="U4B29_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2105436.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNHVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHIDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            21502..23079
                     /locus_tag="U4B29_000467"
     CDS             21502..23079
                     /locus_tag="U4B29_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2105437.1"
                     /translation="MLEFSVIERGGYIPAVEKNKAFLRADGWNDYSFVTMFYLTVFDE
                     HGEKCDIGNVKIGFVGQKEEVSTYSLIDKKFSQLPEMFFSLGESIDYYVNLSKLSDGF
                     KHNLLKAIQDLVVWPNRLADIENESVLNTSLLRGVTLSEIHGQFARVLNGLPELSDFH
                     FSFNRKSAPGFSDLTIPFEVTVNSMPSTNIHAFIGRNGCGKTTILNGMIGAITNPENN
                     EYFFSENNRLIESRIPKGYFRSLVSVSFSAFDPFTPPKEQPDPAKGTQYFYIGLKNAA
                     SNSLKSLGDLRLEFISAFIGCMRVDRKRQLWLEAIKKLSSDENFSNMELISLISKYEE
                     LRRNEPQIQVDDDKFTKLFYDNIQKYLLRMSSGHAIVLFTITRLVDVVGEKSLVLFDE
                     PEVHLHPPLLSAFLRTLSDLLDARNGVAIIATHSPVVLQEVPKSCMWKVLRSREAINI
                     IRPDIETFGENLGVLTREVFLLEVTNSGYHHLLSQSVDSELSYETILKNYNGQIGLEG
                     RTVLKAMIMNRDEGKVQ"
     gene            23076..23966
                     /locus_tag="U4B29_000468"
     CDS             23076..23966
                     /locus_tag="U4B29_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2105438.1"
                     /translation="MKKLPLPARTYSEMLNKCSEGMMQINVRNNFITHFPTFLQKEQQ
                     YRILSSTGQLFTYDRTHPLEPTTLVVGNLTKVKLEKLYENNLRDKNKPARTYYDDMLV
                     SSGEKCPFCGDIGQTKNIDHFLPIAHYPEFSVMPINLVPSCRDCNMGEKGQVFAVDEV
                     HQAIHPYIDKDIFFREQWVYANFVSGTPGAISFYVECPANWRQEDKHRALHHFKLLNI
                     ANRYRLEAGKHLSEVITQRNSFVKVIRKYSSTATFQQLQSEFIEANLKPIIDLNDFPN
                     YWKRVMYQCLANSEDFFRGI"
     gene            24557..25789
                     /locus_tag="U4B29_000469"
     CDS             24557..25789
                     /locus_tag="U4B29_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105439.1"
                     /translation="MTKKPWERRLKDLSHLLKCCIDTYFDPELFRLNLNQFLQTARTV
                     TFIIQKNKNQIIGYDIWYNNNVIEKWKNDPLMAWAKNSRNTIEKQGDLEMYSEAKATL
                     ISSYIEENDIEFITNESMLNIGIKKLVRLAQKKLPSYLTESSIIKSERRWVANTLKDY
                     ELLHALAIIYGRMYNCCNSLGIQINNPMGDDVISPTSFDSLFDEARRITYLKLKDYSI
                     SKLSFSMIQYDNKIIPEDIKERLKLVDKPKNITSTEELVDYTAKLAETTFLKDGYHIQ
                     TLIFYDKQFHPIDLINTTFEDQADKYIFWRYAADRAKITNAYGFIWISELWLRKASIY
                     SNKPIHTMPIIDERLQVIGIDSNNNQKCISWKIVRENEEKKPTLEISTADSKHDEKPY
                     FMRSVLKAIGGDVNTMNN"
     gene            complement(25918..26502)
                     /locus_tag="U4B29_000470"
     CDS             complement(25918..26502)
                     /locus_tag="U4B29_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEN2105440.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENFTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKKSLMQVASEHIAPLQDAADLEIATKEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPAVPVME"
     gene            complement(26502..27446)
                     /locus_tag="U4B29_000471"
     CDS             complement(26502..27446)
                     /locus_tag="U4B29_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEN2105441.1"
                     /translation="MTNALAGKQPKNATLTALAGLSTAKNKLPYFAENDAASLTELTQ
                     VGRDILAKNSVADVLEYLGAGENSAFPAGAPIPWPSDIVPSGYVLMQGQAFDKSAYPK
                     LAVAYPSGVLPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASGTDLGTKTTSSF
                     DYGTKTTGSFDYGTKSTNNTGAHAHSLSGSTGAAGAHAHTSGLRMNSSGWSQYGTATI
                     TGSLSTVKGTSTQGIAYLSKTDSQGSHSHSLSGTAVSAGAHAHTVGIGAHQHPVVIGA
                     HAHSFSIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(27620..28825)
                     /locus_tag="U4B29_000472"
     CDS             complement(27620..28825)
                     /locus_tag="U4B29_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HEN2105442.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKARRNSTTVVVNTVGSENPD
                     EAGRYSMDVEYGQYSVILQVDGFPPSHAGTITVYEDSQPGTLNDFLCAMTEDDARPEV
                     LRRLELMVEEVARNASVVAQSTADAKKSAGDASASAAQVAALVTDATDSARAASTSAG
                     QAASSAQEASSGAEAASAKATEAEKSAAAAESSKNAAATSAGAAKTSETNAAASQQSA
                     ATSASTAATKASEAATSARDAVASKEAAKSSETNASSSAGRAASSATAAENSARAAKT
                     SETNARSSETAAERSASAAADAKTAAAGSASTASTKATEAAGSAVSASQSKSAAEAAA
                     IRAKNSAKRAEDIASAVALEDADTTRKGIVQLSSATNSTSETLAATPKAVKVVMDETN
                     RKAHWTVRH"
     gene            complement(28890..29510)
                     /gene="lom"
                     /locus_tag="U4B29_000473"
     CDS             complement(28890..29510)
                     /gene="lom"
                     /locus_tag="U4B29_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein Lom"
                     /protein_id="HEN2105443.1"
                     /translation="MRNVCIAVAVFAALAVTVTPARAEGGHGTFTVGYFQVKPGTLPS
                     LSGGDTGVSHLKGINVKYRYELTDSVGVMASLGFAASKKSSTVMTGEDTFHYESLRGR
                     YVSVMAGPVLQISKQVSAYAMAGVAHSRWSGSTMDYRKTEITPGYMKETTTARDESAM
                     RHTSVAWSAGIQINPAASVVVDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(29572..32970)
                     /locus_tag="U4B29_000474"
     CDS             complement(29572..32970)
                     /locus_tag="U4B29_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEN2105444.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVMGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKTWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTALISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWELKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKQIADIRQVETSTRYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVELTEDNASRLEEFSKEWKDAS
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LSNVTIAENCTINGTLRAEKIVGDIVKAASAAFPRQRESSVDWPSGTRTVTVTDDHPF
                     DRQIVVLPLTFRGSKRTVSGRTTYSMCYLKVLMNGAVIYDGAANEAVQVFSRIVDMPA
                     GRGNVILTFTLTSTRHSADIPPYTFASDVQVMVIKKQALGISVV"
     gene            complement(33031..33702)
                     /locus_tag="U4B29_000475"
     CDS             complement(33031..33702)
                     /locus_tag="U4B29_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEN2105445.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGV
                     FQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKAR
                     TPRIQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQV
                     VVIGR"
     gene            complement(33600..34343)
                     /locus_tag="U4B29_000476"
     CDS             complement(33600..34343)
                     /locus_tag="U4B29_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2105446.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(34349..35047)
                     /locus_tag="U4B29_000477"
     CDS             complement(34349..35047)
                     /locus_tag="U4B29_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEN2105447.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(35047..35376)
                     /locus_tag="U4B29_000478"
     CDS             complement(35047..35376)
                     /locus_tag="U4B29_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2105448.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(35373..37934)
                     /locus_tag="U4B29_000479"
     CDS             complement(35373..37934)
                     /locus_tag="U4B29_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2105449.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVSAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAYKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSSVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(37927..38361)
                     /locus_tag="U4B29_000480"
     CDS             complement(37927..38361)
                     /locus_tag="U4B29_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HEN2105450.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(38343..38765)
                     /locus_tag="U4B29_000481"
     CDS             complement(38343..38765)
                     /locus_tag="U4B29_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HEN2105451.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(38781..39521)
                     /locus_tag="U4B29_000482"
     CDS             complement(38781..39521)
                     /locus_tag="U4B29_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2105452.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            complement(39529..39924)
                     /locus_tag="U4B29_000483"
     CDS             complement(39529..39924)
                     /locus_tag="U4B29_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2105453.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(39921..40499)
                     /locus_tag="U4B29_000484"
     CDS             complement(39921..40499)
                     /locus_tag="U4B29_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2105454.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(40511..40864)
                     /locus_tag="U4B29_000485"
     CDS             complement(40511..40864)
                     /locus_tag="U4B29_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HEN2105455.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(40876..41274)
                     /locus_tag="U4B29_000486"
     CDS             complement(40876..41274)
                     /locus_tag="U4B29_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HEN2105456.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELKEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(41316..42341)
                     /locus_tag="U4B29_000487"
     CDS             complement(41316..42341)
                     /locus_tag="U4B29_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEN2105457.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(42397..42729)
                     /locus_tag="U4B29_000488"
     CDS             complement(42397..42729)
                     /locus_tag="U4B29_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEN2105458.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(42739..44058)
                     /locus_tag="U4B29_000489"
     CDS             complement(42739..44058)
                     /locus_tag="U4B29_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEN2105459.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISSLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQVVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(44039..45640)
                     /locus_tag="U4B29_000490"
     CDS             complement(44039..45640)
                     /locus_tag="U4B29_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2105460.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(45637..45843)
                     /locus_tag="U4B29_000491"
     CDS             complement(45637..45843)
                     /locus_tag="U4B29_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HEN2105461.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(45840..47765)
                     /locus_tag="U4B29_000492"
     CDS             complement(45840..47765)
                     /locus_tag="U4B29_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEN2105462.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(47740..48285)
                     /locus_tag="U4B29_000493"
     CDS             complement(47740..48285)
                     /locus_tag="U4B29_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HEN2105463.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT        11952 a        12791 c        11335 g        12321 t
ORIGIN      
        1 ccgtaaagtg ataatgatta tcatctacat atcacaacgt gcgtggaggc catcaaacca
       61 cgtcaaataa tcaattatga cgcaggtatc gtattaattg atctgcatca acttaacgta
      121 aaaacaactt cagacaatac aaatcagcga cactgaatac ggggcaacct catgtcaacg
      181 aagaacagaa cccgcagaac aacaacccgc aacatccgct ttcctaacca aatgattgaa
      241 caaattaaca tcgctcttga gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa
      301 gcctgccgtc ggagactaac gtcagaaaag agagcatata catcaattaa aagtgatgaa
      361 gaatgaacat cccgcgttct tccctccgaa caggacgata ttgtaaattc acttaattac
      421 gagggcattg cagtaattga gttgcagttt taccactttc ctgacagtga cagactgcgt
      481 gttggctctg tcacagacta aatagtttga atgattagca gttatggtga tcagtcaacc
      541 accagggaat aatccttcat attattatcg tgcttcacca acgctgcctc aattgctctg
      601 aatgcttcca gagacacctt atgttctata catgcaatta caacatcagg gtaactcata
      661 gaaatggtgc tattaagcat attttttaca cgaatcagat ccacggaggg atcatcagca
      721 gattgttctt tattcatttt gtcgctccat gcgcttgctc ttcatctagc ggttaaaata
      781 ttacttcaaa tctttctgta tgaagatttg agcacgttgg ccttacatac atctgtcggt
      841 tgtatttccc tccagaatgc cagcaggacc gcactttgtt acgcaaccaa tactattaag
      901 tgaaaacatt cctaatattt gacataaatc atcaacaaaa cacaaggagg tcagaccaga
      961 ttgaaacgat aaaaacgata atgcaaacta cgcgccctcg tatcacatgg aaggttttac
     1021 caatggctca ggttgccatt tttaaagaaa tattcgatca agtgcgaaaa gatttagact
     1081 gtgaattgtt ttattctgaa ctaaaacgtc acaacgtctc acattatatt tactatctag
     1141 ccacagataa tattcacatc gtgttagaaa acgataacac cgtgttaata aaaggactta
     1201 aaaaggttgt aaatgttaaa ttctcaagaa acacgcatct tatagaaacg tcctatgata
     1261 ggttgaaatc aagagaaatc acatttcagc aatacaggga aaatcttgct aaagcaggag
     1321 ttttccgatg ggttacaaat atccatgaac ataaaagata ttactatacc tttgataatt
     1381 cattactatt tactgagagc attcagaaca ctacacaaat ctttccacgc taaatcataa
     1441 cgtccggttt cttccgtgtc agcaccgggg cgttggcata atgcaatacg tgtacgcgct
     1501 aaaccctgtg tgcatcgttt taattattcc cggacactcc cgcagagaag ttccccgtca
     1561 gggctgtgga catagttaat ccgggaatac aatgacgatt catcgcacct gacatacatt
     1621 aataaatatt aacaatatga aatttcaact cattgtttag ggtttgttta attttctaca
     1681 catacgattc tgcgaacttc aaaaagcatc gggaataaca ccatgaaaaa aatgctactc
     1741 gctactgcgc tggccctgct tattacagga tgtgctcaac agacgtttac tgttcaaaac
     1801 aaaccggcag cagtagcacc aaaggaaacc atcacccatc atttcttcgt ttctggaatt
     1861 gggcagaaga aaactgtcga tgcagccaaa atttgtggcg gcgcagaaaa tgttgttaaa
     1921 acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt ttattacttt aggcatttat
     1981 actccgctgg aagcgcgtgt gtattgctca caataattgc atgagttgcc catcgatatg
     2041 ggcaactcta tctgcactgc tcattaatat acttctgggt tccttccagt tgtttttgca
     2101 tagtgatcag cctctctctg agggtgaaat aatcccgttc agcggtgtct gccagtcggg
     2161 gggaggctgc attatccacg ccggaggcgg tggtggcttc acgcactgac tgacagactg
     2221 ctttgatgtg caaccgacga cgaccagcgg caacatcatc acgcagagca tcattttcag
     2281 ctttagcatc agctaactcc ttcgtgtatt ttgcatcgag cgcagcaaca tcacgctgac
     2341 gcatctgcat gtcagtaatt gccgcgttcg ccagcttcag ttctctggca tttttgtcgc
     2401 gctgggcttt gtaggtaatg gcgttatcac ggtaatgatt aacagcccat gacaggcaga
     2461 cgatgatgca gataaccaga gcggagataa tcgcggtgac tctgctcata catcaatctc
     2521 tctgaccgtt ccgcccgctt ctttgaattt tgcaatcagg ctgtcagcct tatgctcgaa
     2581 ctgaccataa ccagcgcccg gcagtgaagc ccagatattg ctgcaacggt cgattgcctg
     2641 acggatatca ccacgatcaa tcataggtaa agcgccacgc tccttaatct gctgcaatgc
     2701 cacagcgtcc tgacttttcg gagagaagtc tttcaggcca agctgcttgc ggtaggcatc
     2761 ccaccaacgg gaaagaagct ggtagcgtcc ggcgcctgtt gatttgagtt ttgggtttag
     2821 cgtgacaagt ttgcgagggt gatcggagta atcagtaaat agctctccgc ctacaatgac
     2881 gtcataacca tgatttctgg ttttctgacg tccgttatca gttccctccg accacgccag
     2941 catatcgagg aacgccttac gttgattatt gatttctacc atcttctact ccggcttttt
     3001 tagcagcgaa gcgtttgata agcgaaccaa tcgagtcagt accgatgtag ccgataaaca
     3061 cgctcgttat ataagcgaga ttgctactta gtccggcgaa gtcgagaagg tcacgaatga
     3121 actaggcgat aatggcgcac atcgttgcgt cgattactgt ttttgtaaac gcaccgccat
     3181 tatatctgcc gcgaaggtac gccattgcaa acgcaaggat tgccccgatg ccttgttcct
     3241 ttgccgcgag aatggcggcc aacaggtcat gtttttctgg catcttcatg tcttaccccc
     3301 aataagggga tttgctctat ttaattagga ataaggtcga ttactgatag aacaaatcca
     3361 ggctactgtg tttagtaatc agatttgttc gtgaccgata tgcacgggca aaacggcagg
     3421 aggttgttag cgcgacctcc tgccacccgc tttcacgaag gtcatgtgta aaaggccgca
     3481 gcgtaactat tactaatgaa ttcaggacag acagtggcta cggctcagtt tgggttgtgc
     3541 tgttgctggg cggcgatgac gcctgtacgc atttggtgat ccggttctgc ttccggtatt
     3601 cgcttaattc agcacaacgg aaagagcact ggctaaccag gctcgccgac tcttcacgat
     3661 tatcgactca atgctcttac ctgttgtgca gatataaaaa atcccgaaac cgttatgcag
     3721 gctctaacta ttacctgcga actgtttcgg gattgcattt tgcagacctc tctgcctgcg
     3781 atggttggag ttccagacga tacgtcgaag tgaccaacta ggcggaatcg gtagtaagcg
     3841 ccgcctcttt tcatctcact accacaacga gcgaattaac ccatcgttga gtcaaattta
     3901 cccaatttta ttcaataagt caatatcatg ccgttaatat gttgccatcc gtggcaatca
     3961 tgctgctaac gtgtgaccgc attcaaaatg ttgtctgcga ttgactcttc tttgtggcat
     4021 tgcaccacca gagcgtcata cagcggctta acagtgcgtg accaggtggg ttgggtaagg
     4081 tttgggatta gcatcgtcac agcgcgatat gctgcgcttg ctggcatcct tgaatagccg
     4141 acgcctttgc atcttccgca ctctttctcg acaactctcc cccacagctc tgttttggca
     4201 atatcaaccg cacggcctgt accatggcaa tctctgcatc ttgcccccgg cgtcgcggca
     4261 ctacggcaat aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta
     4321 tttccttcaa gctttgccac accacggtat ttccccgata ccttgtgtgc aaattgcatc
     4381 agatagttga tagccttttg tttgtcgttc tggctgagtt cgtgcttacc gcagaatgca
     4441 gccataccga atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg
     4501 gaaagagagt cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa
     4561 tgaaatttag ctacgctttc gagtctcatg cgccttctcc ctgtacctga atcaatgtta
     4621 ggtttccgca gaacactgcg ccggtatcga tatacatttg gttggcaaac ttgagtggtt
     4681 tcactgctgg cgtatgacca aagatgaacg tgtccgcgcc tttgatttct ttcacgatcc
     4741 cgttttgtga gttgctgatt cgttcgcggt tccagattac ctgctgatga tcaactggct
     4801 ttccaaactc gtattcgtca aagggataat cggcgtggca gataacatat tttttatctt
     4861 tgctcaccag ttcgatgatt aacggaagtt catctgcttt atgggcaaga gctttagcca
     4921 gaatttcttt gtcgtaatcg agattaaaga accagccacc gccattaagc agccagtgat
     4981 taacgtttcc acgctctgat aagccatcaa tcatcatttg ctcatggttt ccacgtacag
     5041 ctctgaacca ggggaatgtg attaattcca ggcattcaac gttctctgca ccacgatcaa
     5101 ccaaatcgcc caccgagata agcaggtctt ttttgttgtc gaatccaatc gtatccagtt
     5161 tgttcatcag gttcgtgtag catccgtgca gatcgccaac tacccaaata tttcggtatt
     5221 tgctgccatc aattttttcg taatagcgca tctctttcac tccatccgcg atgaaccatg
     5281 agaacgtcgt tgacgatggc gtgcattttc ccgtctttat catcaacgta ttttctgacc
     5341 gtaccgcgac tacatttcag tctgcgtgct acttctgtct gatttccgta tgtttcaacg
     5401 agcatgtctg gaatggtttt tactgagaac gtcatgcggc ctcacttctg ctatttcgca
     5461 ggtctttgag tttctgttgg tactctgcct tgatcgcctt gcactcttcg atagtccagc
     5521 gatggcggtt atggtttgat tcgatttcgt ctactgcttc ctgcccgatg cggctaatca
     5581 gttcgacgcg atacggaacg agatttccgc ttttgtgctg gttgcacacc acgcattgct
     5641 tgtgaatatt gcgttcatta aatcggagtt gaggtgccgc agcagttgtc cggtaatgtc
     5701 cggcatccca ctgagcagac gtgagcgttc cgcacgagat acatggtaag tcgcggtctc
     5761 tttctctgat gaaggcgttt acggcttgtt gggcttgttt aatccagtaa ctgcggggct
     5821 ttaaggcgag ttttcgaatc ttaagtttat ctttctgttt ctgctcctct cgtcgtcgtt
     5881 tcttctctgc tgctttttcc gctttttcgc gttctttact tcgtcgttcg agtgctatct
     5941 tggttccaca ctctggagag caccaccact gattagcgaa tgcagggtga aaccattccc
     6001 ggcattcatc gtttttacat cgtcttcgcg ctggtttagc catcatcttc ttcctcgtgc
     6061 atcgagctat tcggatcgct catcagttct gcgcagcagt gctcacacac gtgaacttcc
     6121 agcacatgca gcttctgacc gcagttagcg cacgttaaag ctcgctcgac gctttcttgt
     6181 tcgtaacttc gattttggtc aatcaccttg ttttcctcgc acgacgtctt agccaccgga
     6241 tatcccacag gtgagccgtg tagttgaagg tttttacgtc agattctttt gggattggct
     6301 tgggtttatt tctggtgcgt ttcgttggaa ggtatttgca gttttcgcag attatgtcgg
     6361 tgatacttcg tcgctgtctc gccacacgtc ctccttttcc tgcggtagtg gtaacacccc
     6421 tgttggtgtt ctttcacacc ggagacacca tcgattccag taaggttgat ttggtcggaa
     6481 gcggttatct tctttgcatt caccgcaccg ataacatcgc atcatgcagc ttccctcccg
     6541 aagtcgaaat caagctgccc tccaaatatt tcgcatgact cagaacaaga gccggtatcg
     6601 aatcttttag ctcgtaccat gtcctgatac agggcttgat aatcattttc tgaatacatt
     6661 ttcgcgatac cgtccagcga cattcttcct cggtacataa tctcctttgg cgtttcccga
     6721 tgtccgtcac gcacatggga tcccgtgatg acctcattaa aaacacgctg caatccctcc
     6781 tcatctttgc aggcaagtcc gattttttgc gttgattttt taatgcagaa tatgcagtta
     6841 ccgagatgtt ccggtatttg caaatcgaat ggttgttgct tccaccatgc gaggatatct
     6901 tccttctcaa agtctgacag ttcagcaaga tatctgattc caggctttgg ctttagccgc
     6961 ttcggttcat cagctctgat gccaatccac gtggtgtaat tccctcgccc gaaatggtca
     7021 tcacagtatt tggtgaaggg aacgagtttt aatctgtcag tgcagaacgc gccgccgacg
     7081 tatggagtgc catatttctt taccatatcg ataaatggct tcagaacagg cattcgcgtc
     7141 tgaatatcct ttggttccca taccgtataa ccatttggct gtccaagctc cgggttgata
     7201 tcaacctgca atacggtgag cggtatatcc cagaacttca caacttccct gacaaaccga
     7261 tatgtcattg gatgttcaca acctgtatcc atgaaaacgt aatgcacgtc tttacctgcc
     7321 cgtcgctttt gctccattag ccagagcaaa tatgctgacg tcctgccacc ggagaaacta
     7381 acgacattta tcatgcagcc ctgtctcccc atctcgcttt ccactccaga gccagtctcg
     7441 cttcgtctga ccacttaacg ccacgctctg taccgaatgc ctgtataagc tctaatagct
     7501 ccgcaaattc gcctacacgc atcctgctgg ttgactggcc tattaccaca aagccattcc
     7561 cggcaaggtt aggaacaaca tcctgctgct ttaatgctgc ggtaaacaca cacttccagc
     7621 tttctgcatc cagccagcga ccatgccatt caacctgacg agagacgtca cctaagcagg
     7681 cccatagctt cctgttttgg tctaagctgc ggttgcgttc ctgaatggtt actacgattg
     7741 gtttggttgg gtctggaagg atttgctgta ctgcgtgaat agcgttttgc tgatgtgctg
     7801 gagatcgaat ttcaaaggtt agttttttca tgacttccct ctcccccaaa taaaaaggcc
     7861 tgcgattacc agcaggcctg ttattagctc agtaatgtag atggtcatct tttaactcca
     7921 tataccgcca atacccgttt catcgcggca ctctggcgac actccttaaa aaccaggttc
     7981 gtgctcatct ttccttcccg ttcttccctg gtagcaaacc ggtaatacac cgttcgccag
     8041 accttacctt cgataaccag aagacctgcc cgtgccattt tagccgcggc ctgatttatg
     8101 ctggttactg ttgcgcctgt tagcgcggca acgtccggcg cacagaagct attatgcgtc
     8161 cccaggtaat gaataattgc ctctttgccc gtcatacact tgctcctttc agtccgaact
     8221 tagctttgat ttctgcgatc ttcgccagag cctgtgcacg atttagaggt ctaccgccca
     8281 tgacaggaag ttgttttact ggttcaggga tcgcctcacc acggttaatt ctcgcagtca
     8341 tatggacaag ctcatctgcg gccttacggc gtaattccgc atcagtaagc gcattggccc
     8401 gcatgttctg atacaggttg gtaaccagcc agtagtgcgc gtttgatttc cacggataag
     8461 actccgcatc cggatacagg cctcgcttcc ggcaatactc gtaaaccata tcaaccagct
     8521 cgctgacgtt tggcagtccg gcggtaacgg atgcttcttc ccggcaccat gcaacaaact
     8581 gcccgggtga tggcagaaat ggtcgattct gccgacgggc tacgcgcatt cctgcgttaa
     8641 cctgttccat cgtggtgatc ccgttttccc gaaaagccag aacccactgg cgacggattt
     8701 cgttcacttc gttctggtca cggttagcca ggctcgccgg gaaagttgcc agtaactggc
     8761 tgaacacacc gttgatgatc tgcgctacct gctgtacctg cggcttttcg tcgtactgtt
     8821 ccggcatgtt gttggcgatc cgacgcatct gctcacggtc aaagttaacc atctgtgcgg
     8881 cgatgttttt catagatcca ccccgtaaat ccagtctgtg tttgtcaggt cgagttttgg
     8941 tttgctggct gtcacgcctg cctgttgctt gttacggttg atttcgagtt gggtccactt
     9001 atcgcggagt ttggccgggc tcagcacgtt accggaccag aagttgtcct ggcatgccca
     9061 gcggaacagc acacacatgt cgcggtggtt acgtccgtca cgttcacgca tcaggcggat
     9121 atcgttagcc cacccagcaa aattcggttt tctggctgat ggtgcgatag tcttcaccat
     9181 gtcaaacatc cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc tgccgctctg
     9241 aattgcagca tccggtttca ccacagaaag gtcgttttct ggctggtcag aggattcgcc
     9301 agaattctct gacgaataat cttttctttt ttcttttgta atagtgtctt ttgtgtcccc
     9361 ctgttttgag ggatagcaat cccccaattt gagggatgtt ttatccctcg ttttagggga
     9421 ttttccctcg ttttgaggga tgcaccattc tgagatgttt ttatttggtc caaacatgcc
     9481 gccttgctgc ttgataatat tcattctgac gagttctaac ttggcttcat tgcaccgttt
     9541 gacaggtaac tttgtaatct cgctaagttg agaatcggtg attctgtcca ttggtttatt
     9601 ccacccatag gttttacgca gaatggcaag cagcacttta aactgtcgct tggtcagatc
     9661 tgcgcccgaa taagcctcaa gcagcatatt tgatagtctg gcgtaaccat catcgagatc
     9721 tgccacatta cgctcctgtc cggcaaagtt acctctgccg aagttgagta tttttgctgt
     9781 atttgtcata atgactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     9841 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcgc agcaacttgt
     9901 cgcgccaatc gagccatgtc gtcgtcaacg accccccatt caagaacagc aagcagcatt
     9961 gagaactttg gaatccagtc cctcttccac ctgctgatct gcgacttatc aacgcccaca
    10021 gcttccgctg tcttctcagt tccaagcatt gcgattttgt taagcaacgc actctcgatt
    10081 cgtagagcct cgttgcgttt gtttgcacga accatatgta agtatttcct tagataacaa
    10141 ttgattgaat gtatgcaaat aaatgcatac accataggtg tggtttaatt tgatgccctt
    10201 tttcagggct ggaatgtgta agagcggggt tatttatgct gttgtttttt tgttactcgg
    10261 gaagggcttt acctcttccg cataaacgct tccatcagcg tttatagtta aaaaaatctt
    10321 tcggcctgca tgaatggcct tgttgatcgc gctttgatat acgccgagat ctttagctgt
    10381 cttggtttgc ccaaagcgca ttgcataatc tttcagggtt atgcgttgtt ccatacaacc
    10441 tccttagtac atgcaaccat tatcaccgcc agaggtaaaa tagtcaacac gcacggtgtt
    10501 agatatttat cccttgcggt gatagattta acgtatgagc acaaaaaaga aaccattaac
    10561 acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga
    10621 acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg
    10681 tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc ttacaaaaat
    10741 tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta
    10801 tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca
    10861 tgttcaggca gggatgttct cacctaagct tagaaccttt accaaaggtg atgcggagag
    10921 atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa
    10981 ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt
    11041 tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga
    11101 gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc
    11161 acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag
    11221 tcagtggcct gaagagacgt ttggctgatc ggcaaggtgt tctggtcggc gcatagctga
    11281 taacaattga gcaagaatct tcatcgaatt aggggaattt tcactcccct cagaacataa
    11341 catagtaaat ggattgaatt atgaagaatg gtttttatgc gacttaccgc agcaaaaata
    11401 aagggaaaga taagcgctca ataaacctgt ctgttttcct taattctctg ctggctgata
    11461 atcatcacct gcaggttggc tccaattatt tgtatattca taaaatcgat ggaaaaactt
    11521 ttctctttac caaaacaaat gacaagagtc tggttcagaa gataaatcgc tctaaagctt
    11581 cagttgaaga tattaagaac agcctcgcag atgacgaatc attgggattc ccatcttttt
    11641 tgtttgttga aggcgacacc attggttttg ccagaactgt tttcgggccg accacatccg
    11701 atctgacaga ttttttaatc gggaaaggaa tgtcattaag cagtggagag cgcgttcaga
    11761 tagagccact gatgagggga accaccaaag acgatgttat gcatatgcat ttcatcggcc
    11821 gaacaacggt gaaggtagaa gccaagctac ctgtatttgg cgatatatta aaggtcttag
    11881 gggcaacaga tattgaaggg gagctttttg actcattgga tatagtcatt aagccaaaat
    11941 ttaaaaggga tataaaaaag gttgccaagg atattatttt taacccgtca cctcaatttt
    12001 cagacattag cctgcgggca aaagatgagg ccggagatat tttaacagaa cattatctat
    12061 cagaaaaagg ccatctctca gcgcctctga acaaggtcac caatgctgag atagctgaag
    12121 agatggcata ttgctacgca agaatgaaaa gtgatatact ggaatgtttt aaaaggcagg
    12181 tgggcaaagt taaggattaa ttatcaggag taattatgcg gaacagaatc atgcctggtg
    12241 tttacatagt aataattcct tacgttatcg taagcatttg ctatctcctt ttccgccact
    12301 acattcctgg tgtttctttt tcagctcata gagatggtct tggggcgaca ttgtcatcat
    12361 atgcaggaac catgattgca atcctgattg ctgccttgac gtttctaatc ggaagcagaa
    12421 cgcgccgact ggccaagatt agagagtatg ggtatatgac atcggtagtt attgtctatg
    12481 cccttagttt tgttgagctt ggagctttgt ttttctgcgg gttattgctt ctttccagca
    12541 taagcggcta catgataccc actatcgcca tcggcattgc ctctgcatcg ttcattcata
    12601 tatgcatcct tgttttccaa ctatataatt tgaccagaga acaagaataa cccggcctca
    12661 gcgccgggtt ttctttgcct cacgatcgcc cccaaaacac ataaccaatt gtatttattg
    12721 aaaaataaat agatacaact cactaaacat agcaattcag atctctcacc taccaaacaa
    12781 tgcccccctg caaaaaataa attcatataa aaaacataca gataaccatc tgcggtgata
    12841 aattatctct ggcggtgttg acataaatac cactggcggt gatactgagc acatcagcag
    12901 gacgcactga ccaccatgaa ggtgacgctc ttaaaaatta agccctgaag aagggcagca
    12961 ttcaaagcag aaggctttgg ggtgtgtgat acgaaacgaa gcattggccg taagtgcgat
    13021 tccggattag ctgccaatgt gccaatcgcg gggggttttc gttcaggact acaactgcca
    13081 cacaccacca aagctaactg acaggagaat ccagatggat gcacaaacac gccgccgcga
    13141 acgtcgcgca gagaaacagg ctcaatggaa agcagcaaat cccctgttgg ttggggtaag
    13201 cgcaaaacca gttaaccgcc ctattctctc gctgaatcgc aaaccgaaat cacgagtaga
    13261 aagcgcacta aatccgatag accttacagt gctggctgaa taccacaaac agattgaaag
    13321 caacctgcaa cgtattgagc gcaagaatca gcgcacatgg tacagcaagc ctggcgaacg
    13381 cggcataaca tgcagtggac gccagaaaat taagggaaaa tcgattcctc ttatctagtt
    13441 acttagatat tggccttggc tttatctcaa tattatatgg atcatagctg gcaactaatt
    13501 cagtccagta aatatcctca atagggaata atatatgctt tccattccat cgggaaaaag
    13561 ttttgttcaa cacaccaagc tcaatcaact cactaatgta tgggaattgt tttgatgtaa
    13621 ccacatactt cctgccttca ttaagggctg cgcacaaaac catagattgc tcttctgtaa
    13681 ggttttgaat tactgatcgc actttatcgt tttgcatctt aatgcgtttt cttagcttaa
    13741 atcgcttata tctggcgctg gcaatagctg ataatcgatg cacattaatt gctagcgaaa
    13801 atgcaagagc aaagacgaaa acatgccaca catgaggaat accgattctc tcattaacat
    13861 attcaggcca gttatctggg cttaaaagca gaagtccaac ccagataacg atcatataca
    13921 tggttctctc cagaggttca ttactgaaca ctcgtccgag aataacgagt ggatccattt
    13981 ctatactcat caaactgtag gggttgtaat agtttatccg atttctcgct gtaggggtac
    14041 acgagaacca ccgagcctga tgtggttaaa agacaggcac aatctttact accgcaatcc
    14101 actatttaag gtgatatatg gaagaagaat ttgaagagtt cgaagagcat cctcaggatg
    14161 tgatggaaca ataccaggac tatccgtatg actacgacta ttgataaaaa tcaatggtgt
    14221 ggacaattca agcgatgcaa tggatgcaag ctgcaatcgg aatgcatggt taagcctgaa
    14281 gaaatgtttc ctgtaatgga agatgggaaa tatgtcgata aatgggcaat acgaacgacg
    14341 gcaatgattg ccagagaact tggtaaacag aacaacaaag ctgcctgata gtggccttta
    14401 tttttggcat aaataacaga ataaacactg cactgtgtat tcattccaac gagtgaatac
    14461 acggagcaat gtcgctcgta actaaacagg agccgacttg ttctgattat tggaaatctt
    14521 ctttgccctc cagtgtgagg gcgatttttt atctgtgagg atatgaacag atgtcaaaca
    14581 tcaaaaaata catcattgat tacgactgga aagcatcaat agaaattgaa atcgaccatg
    14641 acgtaatgac agaggaaaaa cttcaccaga ttaataattt ctggtcagac tctgaatacc
    14701 gactcaataa acacggctct gtattaaatg ctgtattaat catgctggcg caacatgctc
    14761 tgcttatagc aatttcaagc gacttaaatg catatggtgt tgtgtgtgag ttcgactgga
    14821 atgatggaaa tggtcaggaa ggatggcctc caatggatgg tagcgaagga ataagaatta
    14881 ccgatatcga tacatcagga atatttgatt cagatgatat gactatcaag gccgcctgag
    14941 tgcggtttta ccgcatacca ataacgcttc actcgaggcg tttttcgtta tgtataaata
    15001 aggagcacac catgcaatat gccattgcag ggtggcctgt tgctggctgc ccttccgaat
    15061 ctttacttga acgaatcacc cgtaaattac gtgacggatg gaaacgcctt atcgacatac
    15121 ttaatcagcc aggagtccca aagaatggat caaacactta tggctatcca gactaaattc
    15181 actatcgcca cttttattgg cgatgaaaag atgtttcgtg aagccgtcga cgcttataaa
    15241 aaatggatat taatactgaa actgagatca agcaaaagca ttcactaacc ccctttcctg
    15301 ttttcctaat cagcccggca tttcgcgggc gatattttca cagctatttc aggagttcag
    15361 ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcactacc
    15421 agcagctcgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa aaaggcctgc
    15481 cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg gccagcaaaa
    15541 aatccattac ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg gcagaacaca
    15601 tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa
    15661 acgaatgagt actgcactcg caacgctggc tgggaagctg gctgaacgtg tcggcatgga
    15721 ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc
    15781 cagcgatgcg cagttcatcg cattactgat cgttgccaac cagtacggcc ttaatccgtg
    15841 gacgaaagaa atttacgcct ttcctgataa gcagaatggc atcgttccgg tggtgggcgt
    15901 tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg actttgagca
    15961 ggacaatgaa tcctgtacat gccggattta ccgcaaggac cgtaatcatc cgatctgcgt
    16021 taccgaatgg atggatgaat gccgccgcga accattcaaa actcgcgaag gcagagaaat
    16081 cacggggccg tggcagtcgc atcccaaacg gatgttacgt cataaagcca tgattcagtg
    16141 tgcccgtctg gccttcggat ttgctggtat ctatgacaag gatgaagccg agcgcattgt
    16201 cgaaaatact gcatacactg cagaacgtca gccggaacgc gacatcactc cggttaacga
    16261 tgaaaccatg caggagatta acactctgct gatcgccctg gataaaacat gggatgacga
    16321 cttattgccg ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt cagaactgac
    16381 acaggccgaa gcagtaaaag ctcttggatt cctgaaacag aaagccgcag agcagaaggt
    16441 ggcagcatga caccggacat tatcctgcag cgtaccggga tcgatgtgag agctgtcgaa
    16501 cagggggatg atgcgtggca caaattacgg ctcggcgtca tcaccgcttc agaagttcac
    16561 aacgtgatag caaaaccccg ctccggaaag aagtggcctg acatgaaaat gtcctacttc
    16621 cacaccctgc ttgctgaggt ttgcaccggt gtggctccgg aagttaacgc taaagcactg
    16681 gcctggggaa aacagtacga gaacgacgcc agaaccctgt ttgaattcac ttccggcgtg
    16741 aatgttactg aatccccgat catctatcgc gacgaaagta tgcgtaccgc ctgctctccc
    16801 gatggtttat gcagtgacgg caacggcctt gaactgaaat gcccgtttac ctcccgggat
    16861 ttcatgaagt tccggctcgg tggtttcgag gccataaagt cagcttacat ggcccaggtg
    16921 cagtacagca tgtgggtgac gcgaaaaaat gcctggtact ttgccaacta tgacccgcgt
    16981 atgaagcgtg aaggcctgca ttatgtcgtg attgagcggg atgaaaagta catggcgagt
    17041 tttgacgaga tcgtgccgga gttcatcgaa aaaatggacg aggcactggc tgaaattggt
    17101 tttgtatttg gggagcaatg gcgatgacgc atcctcacga taatatccgg gtaggcgcaa
    17161 tcactttcgt ctactccgtt acaaagcgag gctgggtatt tcccggcctt tctgttatcc
    17221 gaaatccact gaaagcacag cggctggctg aggagataaa taataaacga ggggctgtat
    17281 gcacaaagca tcttctgttg agttaagaac gagtatcgag atggcacata gccttgctca
    17341 aattggaatc aggtttgtgc caataccagt agaaacagac gaagaatttc atacgttagc
    17401 cgcatccctt tcacaaaagc tggaaatgat ggtggcgaaa gcagaagcag atgagagaaa
    17461 ccaggtatga caaccacgga atgcattttt ctggcagcgg gcttcatatt ctgtgtgctt
    17521 atgcttgccg acatgggact tgttcaatga cacctcagca ggaaaacgcc cttcgcagca
    17581 ttgcccgtca ggctaattct gaaatcaaaa aaagccagac agcagtttcc ggataaaaac
    17641 gtcgatgaca tttgccgtag cgtactgaag aagcaccgcg aaacggtaac gctgatggga
    17701 ttcacaccga ctcatttaag cctggcaatc ggcatgttaa acggcgtctt taaggaacga
    17761 tgaacatgaa aagcaaaatc atcagggagc tacaggctcc ttttttatta ttcgcattca
    17821 ccctcaagcg tattaaccaa cagttcaggg attaatgaaa gatggcagac atcattgatt
    17881 cagcatcaga aatagaagaa ttacagcgca acacagcaat aaaaatgcgc cgcctgaacc
    17941 accaggctat atctgccact cattgttgtg agtgtggcga tccgatagat gaacgaagac
    18001 gcctggtcgt tcagggttgt cggacttgtg caagttgcca ggaggatctg gaacttatca
    18061 gtaaacagag aggttcgaag tgagcgaaat taactctcag gcactgcgtg aagcggcaga
    18121 gcaggcaatg catgacgact ggggatttga cgcagacctt ttccatgaat tggtaacacc
    18181 atcgattgtg ctggaactgc tggatgaacg ggaaagaaac cagcaataca tcaaacgccg
    18241 cgaccaggag aacgaggata ttgcgctaac agtagggaaa ctgcgtgttg agcttgaaac
    18301 agcaaaatca aaactcaacg agcagcgtga gtattacgaa ggtgttatct cggatgggag
    18361 taagcgtatt gctaaactgg aaagcaacga agtccgtgaa gacggaaacc agtttcttgt
    18421 tgttcgccat cctgggaaga ctcctgttat caagcactgc actggtgacc tggaagagtt
    18481 tctgcggcag ttaatcgaac aagacccgtt agtaactatc gacatcatta cgcatcgcta
    18541 ttacggggtt ggaggtcaat gggttcagga tgcaggtgag tatctgcata tgatgtctga
    18601 cgctggcatt cgcatcaaag gagagtgaga tcggttttgt aaaagataac gcttgtgaaa
    18661 atgctgaatt tcgcgtcgtc ttcacagcga tgccagagtc tgtagtgtca gatgatgacc
    18721 gtactcaaac atcgggttga gtattatctt actgtttctt tacataaaca ttgctgatac
    18781 cgtttagctg aaacgacata cattgcaagg agtttataaa tgagtatcaa tgagttagag
    18841 tctgagcaaa aagattgggc gttatcaatg ttgtgcagat ccggtgtctt gtctccatgc
    18901 agacatcacg aaggtgttta tgtagatgaa ggtatagata tagagtcggc atacaaatat
    18961 tccatgaagg tttataagtc taatgaagac aaatccccat tctgcaatgt gcgagaaatg
    19021 actgataccg tgcaaaatta ttatcacgag tacggtggaa acgatacttg ccctctctgt
    19081 acaaaacata tagatgatta aacccaatat tacataacaa tcctcgcact cgcggggatt
    19141 tattttatct gaactcgcta cggcgggttt tgttttatgg agatgataaa tgcacttccg
    19201 agtcacagga gaatggaatg gagagccatt caacagagtt atcgaagcgg agaacatcaa
    19261 cgactgctac gaccactgga tgatatgggc gcagatagca catgcagacg taaccaatat
    19321 tcgaattgaa gaactgaaag aacaccaagc cgcctgatgg cggttttttc ttgcgtgtaa
    19381 ttgcggagac tttgcgatgt acttgacact tcaggagtgg aacgcacgcc agcgacgtcc
    19441 aagaagcctt gaaacagttc gtcgatgggt tcgggaatgc aggatattcc cacctccggt
    19501 taaggatgga agagagtatc tgttccacga atcagcggta aaggttgact taaatcgacc
    19561 agtaacaggt ggccttttga agaggatcag aaatgggaag aaggcgaagt catgagcgcc
    19621 gggatttacc ccctaacctt tatataagaa acaatggata ttactgctac agggacccaa
    19681 ggacgggtaa agagtttgga ttaggcagag acaggcgaat cgcaatcact gaagctatac
    19741 aggccaacat tgagttattt tcaggacaca aacacaagcc tctgacagcg agaatcaaca
    19801 gtgataattc cgttacgtta cattcatggc ttgatcgcta cgaaaaaatc ctggccagca
    19861 gaggaatcaa gcagaagaca ctcataaatt acatgagcaa aattaaagca ataaggaggg
    19921 gtctgcctga tgctccactt gaagacatca ccacaaaaga aattgcggca atgctcaatg
    19981 gatacataga cgagggcaag gcggcgtcag ccaagttaat cagatcaaca ctgagcgatg
    20041 cattccgaga ggcaatagct gaaggccata taacaacaaa ccatgtcgct gccactcgcg
    20101 cagcaaaatc agaggtaagg agatcaagac ttacggctga cgaatacctg aaaatttatc
    20161 aagcagcaga atcatcacca tgttggctca gacttgcaat ggaactggct gttgttaccg
    20221 ggcaacgagt tggtgattta tgcgaaatga agtggtctga tatcgtagat ggatatcttt
    20281 atgtcgagca aagcaaaaca ggcgtaaaaa ttgccatccc aacagcattg catattgatg
    20341 ctctcggaat atcaatgaag gaaacacttg ataaatgcaa agagattctt ggcggagaaa
    20401 ccataattgc atctactcgt cgcgaaccgc tttcatccgg cacagtatca aggtatttta
    20461 tgcgcgcacg aaaagcatca ggtctttcct tcgaagggga tccgcctacc tttcacgagt
    20521 tgcgcagttt gtctgcaaga ctctatgaga agcagataag cgataagttt gctcaacatc
    20581 ttctcgggca taagtcggac accatggcat cacagtatcg tgatgacaga ggcagggagt
    20641 gggacaaaat tgaaatcaaa taatgatttt attttgactg atagtgacct gttcgttgca
    20701 acaaattgat aagcaatgct tttttataat gccaacttag tataaaaaag ctgaacgaga
    20761 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
    20821 ataatactgt aaaacacaac atatgcagtc actatgaatc aactacttag atggtattag
    20881 tgacctgtaa cagagcatta gcgcaaggtg atttttgtct tcttgcgcta attttttgtc
    20941 atcaaacctg tcgcactcca gagaagcaca aagcctcgca atccagtgca aagctttgtg
    21001 tgccacccac tacgacctgc ataaccagta agaagatagc agtgatgtca aacgacgcag
    21061 ctgacttctt ttctttcacg acttccccac acccagcatg catacctttc cgccataact
    21121 gtagtgaatg tctgttatga gcgaggagcg gaagttaaca cttatgaaaa atggctacga
    21181 agtccgtggc tatctatcgg cttattagta cttgaaacgc ttcttcagaa gcctgaagag
    21241 ctaatcgttc ggcgatacta tatatgcatt aatagactat atcgttggta taaacagtgc
    21301 accatgcaac atgaataaca gtgggttatc caaaaggaag cagaaagcta aatatggaaa
    21361 actacaatac gatgccccgt taagttcaat actactaatt tttagatgga aaacgtatgt
    21421 aatagagagt aacttaaaag agagatcctg tgttgccgcc aaataaattg cggttatttt
    21481 aataaaatta agggttacta tatgttggag tttagtgtta ttgaaagagg cgggtatatt
    21541 cctgcagtag aaaaaaataa ggcattccta cgagcagatg gttggaatga ctattccttt
    21601 gttacaatgt tttatcttac tgtctttgat gagcatggtg aaaaatgcga tatcggaaat
    21661 gttaaaattg gttttgtagg tcaaaaagaa gaagtaagca cttattcatt aatagataaa
    21721 aaattcagtc aactccctga aatgtttttt tccttaggtg aaagcattga ctactatgtt
    21781 aatctcagca aattaagcga tggttttaaa cataaccttc ttaaagctat tcaggattta
    21841 gtagtatggc caaatcgatt agccgacatt gaaaatgaaa gcgtccttaa cacctcatta
    21901 cttagagggg taactctttc agaaattcat ggacagttcg cacgtgtgtt aaatggtttg
    21961 ccagaattgt cagatttcca cttttcattt aatagaaaaa gtgctcccgg attcagtgat
    22021 ttaactatac cttttgaggt gacggttaat tctatgccca gcacgaacat tcatgctttt
    22081 atcgggcgga atgggtgtgg taaaacaaca attttgaatg gaatgattgg tgcaatcacc
    22141 aacccagaaa acaatgaata ttttttctct gaaaataata gacttatcga gtcaagaatc
    22201 ccaaagggat attttcgatc gcttgtttca gtttcgttta gtgcatttga tccttttact
    22261 cctcctaaag aacaacctga cccagcaaaa ggtacacaat acttttatat tggactcaag
    22321 aatgctgcca gcaatagttt aaaatcacta ggcgatctcc gcttagaatt catttcagca
    22381 tttattggtt gtatgagagt agatagaaaa agacaactct ggcttgaagc tatcaaaaaa
    22441 ctaagtagtg atgaaaactt ttcaaatatg gaactcatca gcctcatttc taaatatgaa
    22501 gagttaagac gtaatgaacc acagattcaa gtggacgatg ataaattcac taaattgttt
    22561 tatgacaata tccagaaata tctgcttcga atgagctctg gacatgcaat tgttttattt
    22621 actatcacaa gattagtaga tgtcgttggc gaaaagtcat tagttttatt cgatgaacca
    22681 gaggttcatc tgcatccacc tttgctctct gcttttttac gaacattaag cgacttactc
    22741 gatgcacgca atggtgtagc aataattgca actcattccc cagtagtact gcaagaggtt
    22801 ccaaaatcct gcatgtggaa agtcctacgg tcaagagaag caataaatat tatccgtccg
    22861 gatattgaga cattcggtga gaacttaggt gttttaactc gtgaggtgtt tttacttgaa
    22921 gtgacaaatt ctggatacca ccacttatta tcgcagtccg ttgattcaga gctttcttat
    22981 gaaaccattc taaaaaatta taatggtcag ataggattag aaggtcgaac cgttttaaaa
    23041 gcgatgataa tgaacagaga tgaaggtaaa gtacaatgaa aaaactacct cttccagcga
    23101 gaacttatag cgaaatgctt aataaatgct cggaaggtat gatgcagata aatgttagaa
    23161 ataatttcat tactcacttc cccacttttt tgcagaaaga acaacaatat agaatattaa
    23221 gctcgacagg tcagttattt acctacgaca ggacacaccc tcttgagcct acaaccttag
    23281 tagttggtaa cctgacaaag gttaaattag aaaagcttta tgaaaataat ctccgagata
    23341 aaaacaaacc cgctagaaca tattacgatg acatgcttgt ttcatcaggt gaaaaatgtc
    23401 cattttgtgg tgatatagga cagacaaaaa atatagatca ttttcttcct attgcacatt
    23461 atcctgaatt ttcggtgatg cctattaatt tagttccatc gtgccgcgac tgcaatatgg
    23521 gagagaaagg tcaagttttc gcagtagatg aggtacacca agcgattcat ccctatatcg
    23581 acaaggacat tttttttcgt gagcaatggg tatatgcaaa tttcgtttcc ggaactccgg
    23641 gtgctatcag tttttatgtt gaatgcccgg cgaactggag gcaggaagac aaacacagag
    23701 ctcttcatca tttcaagcta ttaaatattg ctaacaggta tcgtttggag gcagggaagc
    23761 acttgagtga agtgattact caaagaaact ctttcgtaaa agttataagg aaatatagtt
    23821 caaccgcaac gtttcagcag ctacagtcag aatttattga agcaaatctg aaacctatta
    23881 tagatttgaa tgacttcccc aattattgga aaagagttat gtatcagtgc ctagcaaact
    23941 cggaagattt tttcagaggg atctagaata tgatgaaaga tagaaaatta cgacgcttat
    24001 cggaagtgaa cgaatacttt ttatatgagg agggctgttt ttacaaaatc cggtagtaac
    24061 ttgctaacca attcctaggc aggtcattgg caacagtggc atgcaccgag aaggacgttt
    24121 gtaatgtccg ctccggcaca tagcagtcct agggacagtg gcgtacagtc atagatggtc
    24181 ggtgggaggt ggtacaaatt ctctcatgca aaaaatatgt aaaatcggta gcaactggaa
    24241 atcattcaac acccgcacta tcggaagttc accagccagc cgcagcacgt tcctgcatac
    24301 gacgtgtctg cggctctacc atatctccta tgagcaacgt gttagcagag ccaagccaca
    24361 actctaattt taatacataa tgaatgataa taataatatt aaaaatttcc tgtgtaacta
    24421 atttactata tggtttctga taagaatcat tgcaaagatc aaacaacttg tattacattg
    24481 acagttaagc agttaatttt atcacctcta aaatatatca gcatctagca tgcaacctat
    24541 caaaatggag agttttatga ctaaaaaacc atgggaaaga agacttaaag atttatcgca
    24601 cttgctcaaa tgctgcattg atacatattt tgaccctgaa ttatttcgct tgaatttgaa
    24661 tcaattcctc caaaccgcaa gaacagtaac atttattatt caaaaaaaca aaaaccagat
    24721 tataggatat gacatttggt ataacaataa tgttattgaa aaatggaaaa atgatccatt
    24781 aatggcttgg gctaaaaatt ctcgcaatac gatagaaaaa caaggcgatt tagaaatgta
    24841 tagcgaggca aaggctactc ttatttcatc ttacattgaa gaaaatgaca ttgagtttat
    24901 tacaaatgaa agtatgttaa acattggtat aaaaaagtta gtcagacttg cacaaaagaa
    24961 attaccttca tatttaactg aatcatctat tattaaatca gaaagacgat gggtcgctaa
    25021 tacgctaaaa gattacgaat tattacatgc cttagctata atctatggca gaatgtataa
    25081 ctgctgtaac tctcttggca tacaaataaa caatccaatg ggtgacgatg tgatttcgcc
    25141 aacatcattc gactctttat ttgatgaagc caggagaata acttatttaa aattaaaaga
    25201 ttactccata agcaaattgt catttagcat gatacaatat gacaataaaa taattcctga
    25261 agatattaaa gagcgtctaa aactggtaga taagcctaaa aatatcactt cgacagaaga
    25321 gttagttgac tatacagcca agcttgcaga aacgactttt ttaaaggacg gttatcacat
    25381 tcaaacatta attttttatg ataaacaatt ccatccaatt gatttaatca atacaacatt
    25441 tgaagatcaa gcagataaat atattttttg gcgttatgca gctgacagag ccaaaataac
    25501 aaatgcctat ggcttcattt ggatatcaga gctatggctc agaaaagcaa gcatctactc
    25561 caataaacca atacatacaa tgccaattat agatgaaaga cttcaggtaa ttggaattga
    25621 ttcaaataat aatcaaaaat gtatttcatg gaaaatagtt agagaaaacg aagaaaaaaa
    25681 accgacttta gaaatatcaa cagcagactc aaaacatgac gaaaaaccat atttcatgcg
    25741 ttcagtctta aaagcaattg gcggtgatgt aaacactatg aacaattgag tcatagaact
    25801 tccattattc tcctgaagat aataatcgcc aaataaacca atactcagct ttacaatata
    25861 ctaactaacc gcagaacgtt atttcataca acgtttctgc ggcatatcac aaaacgatta
    25921 ctccataaca gggacagcag gccactcaat atcaggtgca gttgatgtat caacacggtt
    25981 cagcaacacc cgatacttct tccaggcttc cagcaacgag gtttcttcct tcgttgcaat
    26041 ttccagatct gcagcatcct gaagcggcgc aatatgctca ctggctacct gcatcaggct
    26101 tttttttgtt tcttccgcct cccggatccg gaacagtttt tctgcttccg tatccttcac
    26161 ccaggctgtg ccgttccact tctgatattc ccctcccggc gataaccagg taaaattttc
    26221 cggtaacgga ccgagttcag aaataaataa cgcgtcgccg gaagccacgt catagacggt
    26281 tttaccccga tggtcttcaa cgagatgcca cgatgcctca tcactgttga aaacagccac
    26341 aaagccagcc ggaatatctg gcggtgcaat atcggtactg tttgcaggca gaccggtatg
    26401 aggcggaata tatgcgtcac cttcaccaat aaattcatta gttccggcca gcagattata
    26461 aatttttatg gtccgtggtt gttcactcat tctgaatgcc attatgcaag cctcacaata
    26521 tagttaaatg caatgttttt gacggtgttt tccgcgttac ccgcagcgtt aacggtgatg
    26581 gtgtgtccgt gtgaaccaat actgaaagaa tgggcatgag caccgataac aaccggatgc
    26641 tggtgcgcac caataccaac tgtatgcgca tgtgcaccgg cactcacggc tgtaccggac
    26701 aatgagtgac tgtggctgcc ctgactgtcc gttttcgata aataagcaat accctgtgtg
    26761 ctggttcctt taactgtgga taaacttcct gtaatggttg ctgttccata ctgactccag
    26821 ccagaactgt tcatccttaa accacttgtg tgggcatgag cacccgcggc ccctgttgaa
    26881 ccgctcagac tgtgagcatg agcccccgtg ttattcgtcg atttggtgcc gtaatcgaaa
    26941 ctgcctgttg ttttcgtccc gtaatcaaac gacgatgtgg ttttcgtccc caaatccgta
    27001 ccggatgcac tggcactgtg ggtgtgcgac ttaattccat cctgttcctg agacaataca
    27061 gcacgaccgc tggcgggttt ccccttgatt gtccagcctc gcatatcagg aagcacaccc
    27121 gatggatacg cgacagcaag ttttgggtag gctgatttgt caaacgcctg cccctgcatc
    27181 aggacgtagc cagacggaac gatatctgat ggccacggga tcggcgcacc tgccggaaag
    27241 gccgaattct caccggcccc aaggtattca agaacatctg caacggaatt ttttgccaga
    27301 atatccctgc caacctgagt cagttcagtc aggctggcgg catcattttc cgcaaaatac
    27361 ggtaatttat ttttcgccgt ggaaagccct gccagcgccg tcagtgtcgc attcttcggt
    27421 tgtttacccg caagcgcgtt agtcatggtg gtagcaaaat ctggatcatt cccgagcgct
    27481 gcggccagtt cattcagcgt attcagtgcg tcaggtgacg cgtcgataac atctgcaatc
    27541 gcggccagta caaaagcggt gttcgcaatc tgggtattgt ttgttcccct gagcgcggtt
    27601 ggtgctgttg gcgttccggt cagtgccgga ctgtccagtg ggcttttctg ttcgtttcat
    27661 ccattaccac cttaaccgcc tttggcgttg cagcaagcgt ttcagacgtg ctgttggttg
    27721 cactgctgag ctgcactatc ccctttctcg ttgtgtccgc atcctcaagc gcgacagctg
    27781 aagctatatc ttctgcacgt tttgccgaat tttttgcacg tattgccgcc gcttctgccg
    27841 cacttttgct ctgcgatgct gataccgcac ttcccgcagc ctctgtcgcc ttcgtggatg
    27901 ccgttgacgc actccccgcc gccgctgttt ttgcgtctgc cgcggcagag gcgctccgtt
    27961 ccgctgctgt ttcagatgac ctggcattcg tctcggacgt ttttgccgcc ctggcagaat
    28021 tttctgccgc cgttgccgag gaagctgcac gaccggcact tgatgatgcg ttcgtttctg
    28081 atgattttgc tgcctctttt gaggccaccg catctcgtgc tgaagtggcg gcctctgacg
    28141 ctttcgtggc cgcggtggag gcagacgtgg cggctgattg ttgtgacgct gcagcattcg
    28201 tttctgacgt tttcgccgca ccggcactgg tggccgccgc gttttttgag gactctgcgg
    28261 ctgcggcact tttttccgct tcagtggcct ttgctgatgc cgcttctgcg ccggaggacg
    28321 cttcctgagc tgacgatgca gcctgtccgg cggacgtgct ggcggcgcgt gctgagtcag
    28381 ttgcatcagt cacaagggcc gcgacctgag cagctgatgc actggcatcg ccggctgatt
    28441 tcttcgcgtc tgccgtactc tgtgccacca cggacgcgtt acgcgccacc tcttccacca
    28501 tcagttcaag acgacgcagc acctccggcc gggcatcatc ctccgtcatg gcacagagaa
    28561 aatcattcag cgtccccggt tgtgaatctt catacacggt gatggtcccg gcgtgcgatg
    28621 gtggaaaacc gtcaacctgc aggatgacac tgtactgacc gtactccaca tccatgctgt
    28681 aacgcccggc ttcatccgga ttctctgagc ccaccgtgtt caccaccacc gtggtgctgt
    28741 tacgtctggc tttcagctga atggtgcagt tctgtaccgg ttttcctgtg ccgtctttca
    28801 ggactcctga aatctttact gccatattca ccccacaaaa aagcccaccg gttccggcgg
    28861 gctgtcataa cactgtgtta cctggctaat cagaatttat aaccgacccc aacgatgaat
    28921 ccgtcagtac gccagtcgcc actgccggag ccttcataag caatatcaac aacgacggac
    28981 gctgccggat taatctgtat acctgcactc cacgccactg aggtatgccg cattgcactt
    29041 tcgtccctgg cagtggtcgt ctctttcata tacccgggag tgatttccgt cttacggtaa
    29101 tccattgtac tgccggacca ccgactgtga gccactccgg ccatggcgta cgcactgacc
    29161 tgcttactga tttgtaaaac cggtccggcc atcacgctca cataacgtcc acgcaggctc
    29221 tcatagtgaa acgtatcctc cccggtcatc actgtgctgc tctttttcga cgcggcgaac
    29281 cccagggaag ccatcacccc cacactgtcc gtcagctcat aacggtactt cacgttaatc
    29341 cctttcagat gactcacacc ggtatccccg cccgacaacg acggcaatgt acccggtttc
    29401 acttgaaaat agcccaccgt aaacgtacca tgtccacctt ccgcacgggc cggagtgact
    29461 gtcaccgcaa gtgcggcaaa gacagcaacg gcaatacaca cattacgcat cgttcacctc
    29521 tcactgtttt ataataaaac gcccgttccc ggacgaacct ctgtaacaca ctcagaccac
    29581 gctgatgccc agcgcctgtt tcttaatcac cataacctgc acatcgctgg caaacgtata
    29641 cggcggaata tctgccgaat gccgtgtgga cgtaagcgtg aacgtcagga tcacgtttcc
    29701 ccgacccgct ggcatgtcaa caatacggga gaacacctgt accgcctcgt tcgccgcgcc
    29761 atcataaatc accgcaccgt tcatcagtac tttcagataa cacatcgaat acgttgtcct
    29821 gccgctgaca gtacgcttac ttccgcgaaa cgtcagcgga agcaccacta tctggcgatc
    29881 aaaaggatgg tcatcggtca cggtgacagt acgggtacct gacggccagt ccacactgct
    29941 ttcacgctgg cgcggaaaag ccgcgctcgc cgcctttaca atgtccccga cgattttttc
    30001 cgccctcagc gtaccgttta tcgtacagtt ttcagctatc gtcacattac tgagcgtccc
    30061 ggagttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct ttccgtccgg
    30121 tgtcagggaa aaggccggag gattgccgcc gctggtaatg gtgggggccg tcaggcgctt
    30181 caggaacacg tcgttcatga atatctggtt gccctgcgcc acaaacatcg gcgtttcatt
    30241 cccgtttgcc gggtcaataa atgcgatacg attggcggca accagaaact ggctcagttt
    30301 gccttcctcc gtgtcctcca tgctgaggcc aatacccgcg acataatgtt tgccgtcttt
    30361 ggtctgctca attttgacag cccacatggc attccactta tcactggcat ccttccactc
    30421 tttcgaaaac tcctccagtc tgctggcgtt atcctccgtc agctcgactt tttccagcag
    30481 ctccttgccg agatgggatt cggttatctt gcctttgaaa aaatccaggt aaccttccgc
    30541 atcatcgctc gcccgaccga cggcctccac gaatgccgat ttgccaacgg tgttcacact
    30601 gcggatataa aagtaataat catggcccgg tttgatattg atactggcgg ctatccagta
    30661 cagcgccgta ccaagataac gcgtgctggt ttcaacctgt ctgatatccg caatctgctt
    30721 ttccgagaac cagaactcaa actgtaccgt cgggtcataa acggcaagat gcggcgtggc
    30781 ggttatctga aaatagcccg gcgtcagctc aatcctcgac ggtgctgccg gtgcggcaat
    30841 ccggaacgat accgacgccg gatcgccctg ctgcccccac gcatttaccg cccggactgt
    30901 cagcctgtag ttccccagcg ccagttgcgt gaagcggtat gtggtttccg tcgtccgggc
    30961 cgtgctgacc agccgctcac tgccgtcgtc cgctgttacg gtcagacgga gcaggaaact
    31021 cacgcccttc accaccttcg gtgtgtccca tcgcgccagc acctgatatt ccccgctgtc
    31081 tgcagtgact tctgcggtca ggtgctgcac cgctggcggc gtgacaccat tcaccgtgcc
    31141 actctgttcg ccgtcaaagt gcgccccgtt atccacgatg gcctcttttt ccggcacatg
    31201 ctgcacggcg gtgatggcat acgtgccgtc gtcgttctca cggatactca cgcagcggaa
    31261 cagtcgctgg cgcagcgtcg gcagcttcag ctcccatacg ctgtattcag caacaccgtc
    31321 aggaacacgg ctcactttta ccttcacgcc gtcggtgacg gactgaacct ccacgctgac
    31381 cggattgcca cttccgtcaa ccaggcttat cagcgcggta ccggaggatg gcagcgtgat
    31441 ttcacggtcg agcgtcagcg tccgggtctg gctgttcacc gccagcacac gaccaccggt
    31501 gctgataccg gcatagtcat catcgcagat ttcaataaca tcgcccggta catggcgaag
    31561 cccttctgcg ccgacgctga aatccacggt ctgcgtttcc agcagttctg ttttaatcag
    31621 ccacagcccg gcgcggtgtg cctgcccccg gctggtacag ccaaaggcat ccatcttcgt
    31681 aacattacga ccgtaacggg caatggcctg cgtatcttca acaagctctg tcgccgtctc
    31741 ccagccgttg ttcgggtcaa tccagttcac ctcaacggca ttatggcggt ccttcagggc
    31801 gctgaagctg tagcggaacg gcgcgccatc atccggcatc accacattac tgcggttata
    31861 ggtccacgtc ttatccgacg gtcggtcctg cacgaacgtc agcgtctgcc cgttccatac
    31921 cggcatacag cgcatcgccg agcagaaatc gctgagcaca tcccacgcct tacgctgtgt
    31981 ggtcaggtac gcattacagg tgatgcgcgg ctccgtgccg ccaaagccgt ccggcactga
    32041 ctggtcgcag tactggccga tgacatacag cgcccattta tccacatccg ccgcaccaag
    32101 acgtttcccc atgccgtagc gcggatgggt cagcatatcc cacagacacc aggccatgtt
    32161 gttgctgtat gccggtttaa acgttccgtc ccagataccg ctgtattgcc gcgtctgcgg
    32221 gttatagttc gacggcacct gcagaatacg cccgcgcaga tgataattac ggctcacctg
    32281 ctggctgccg aactgctccg agtccacctg cacgccgacc agtgccgtgt tcgggtagca
    32341 ctgtttcaca tcgatgattt cagtgtatga cgaccagagc gttttgttct gcagctggtc
    32401 tgtggtgctg tccggcgtca tcctgcgcat ccggatatta aacgggcgcg gcggcaggtt
    32461 acccatcacc accgaggcca gatactgcga ggtggttttg cccttaatgg tgatgtcttt
    32521 ttccgtcacc cagccaccgt tacgttgtat ctgaaccagc aggcggactt ccgacggatt
    32581 cctgtcaccc tttgaggtgg tttccaccag tgcctgtaca ccgaaggtaa agcgcagacg
    32641 gtcgatgttt gcagacgtaa tggtgcgggt gatcggcgtg tcatatttca cttccgtacc
    32701 cagcaccgtc tcggagccgg aggattcaaa tccctccggc ggagtctgct cctgctcacc
    32761 agcccggaac accaccgtga caccggatat gttggtattc ccctcagtgt ccagcaccgg
    32821 cgtactgttc agcagcacgc tttttaagcc atccaccgga ccttcaatcg gcccttcgct
    32881 gatggcatcg atcacactca gcaactgcgt ggacttcagg ttgtccttcg cttcgcgcgg
    32941 ggtatgcccc ttactgcttc ctttacccat tcctcacgct ccataaatga caaaaccgcc
    33001 cgcaggcggt ttcacataaa acattttgca tcagcgacca atcaccacaa cctgaccacc
    33061 gtccccttcg tctgccgtgc tgatctcctg agaaaccacg cgtgacccca cgcgcatttc
    33121 cccgtacaga acaggcagaa cattgccctg ggcaaccatg ttatccagtg aggagaaata
    33181 ggtgttctgc ttaccgttat ccgttgtctg tatacgggga gttctggctt tcggtgccag
    33241 catctgcgcc acaccaccga gcaccatact ggcaccgaga gaaaacagga tgccggtcat
    33301 accaccggcc ccaatggctg ccccccatgc tgcaagggtg gctccggcgg taaagaatga
    33361 tccggcaatg gcggcagccc ccaggacaat ctggaatacg ccacctgact tggccccggc
    33421 gactctggga acaatatgaa ttacagcgcc atcaggcaga gtctcatgta actgcgccgt
    33481 taacccggac gtgctgacgt cccgcccggc aatccgtacc tgataccagc cgtcgctcag
    33541 tttctgacga aacgccggga gctgtgtggc cagtgcccgg atggcttcag cccccgtttt
    33601 cacacgaagg tcgatgcggc gaccaaatcg ttgtaaatcc ccgtaaaggc agatgcgcgc
    33661 catgcccggt gacgccagag ggagtgtgtg cgtcgctgcc atttgtcggt gtacctctct
    33721 cgtttgctca gttgttcagg aatatggtgc agcagctcgc cgtcgccgca gtaaattgcg
    33781 gcgtgattcg gcactgatga accaaaacag cacagcagca catcgcccgg ctgtgccgct
    33841 gacaacggca cctgatacag ccccgtcgcc tccagattat ccagatagag attctggccg
    33901 ttacgccacc agtcatcctc acgatgaaag tccggcatct caatccccgc cagatgataa
    33961 gcatcccgga acagtgtgta acagtccgtc acaccgtgct caaagcgccg cccggtgaga
    34021 tgcggcacac agcggaactt atgaatcgtc ccccggcaga ccagccacca cggcaaatca
    34081 ctctgcacct gcagccgccg gtcggcctca ctcagccagg gcagaccacc ggggtggctg
    34141 tggaccagcg ccacaatctc accctgcatt tctgcctgca gccagtcttc cggcgacata
    34201 cggaaatacg cctccggctc accggagata ttcacgcagg ggaaatatct ttccccctcc
    34261 ggcgtgctta ccacgaagcc gcacgactcc gctggcgcac atcgccgggc gtgcgccaga
    34321 atcgctgatt ctgtctgtgt catgggattt actgcgaaag tttgttaatg gaaaggaagc
    34381 cgccaaagtt gccgacgtta ttgcggaact tacaaccgct caggcatttg ctgcatttat
    34441 ccttcgtgat atcggacgtt ggctggtcat attcatccgc gacagccgga ccgctataac
    34501 cgcactcgtc accgcgatag gtccaggtgc aggtgttggc cagcatgata cgtcccggaa
    34561 aaacagcgcc atccgtttcc gtcggcgtgg acagtacaaa ggaggcactc accgcgctca
    34621 gttcgctgca ctgctcaatg cgccagcggc tgatcacctc ctgctccgga tcggcgtaac
    34681 tgtttccgtt gacgaagttc accgcatcca gaaaacgggc gtaaacctta cgccggacca
    34741 ccgttccgcc gaccagactc tgcatatctt ccgccatccc ggtgaccata ccgtacaggt
    34801 tagaaaccgt cagcgtgggg cgcgtactgg tgcctttgcc attcagttca aaaccgctcc
    34861 cctgaatggg atacggctga tactgtcgcc cctgccaggt gaccggctca cctttttcgt
    34921 tctgctcatt acagaaaaaa taacgttctc caccgacctc tgtcaggtcg atttcccaga
    34981 gcaccacgct ggccgactgc tccgcacggg tgcattcatt cagtgtttcc tgccggatat
    35041 cctgcatcag ttcaccacct gttcaaactc tgcgctgaac tcaacacgca gcatactgac
    35101 ccgcgacgac cattttgcgc aggtcacctt tatctgccgc cactcataag gcggcgtcca
    35161 cagaaaggat ttccagcccc cgtgctcttc cagaaacgac tccagtaccg tggcctcctc
    35221 acgggggaca gaaagcgtca cgctgtacgt tttcaggttg gcattcagcc cggcaggcgc
    35281 tcgctgagaa tagccatcac caaagcgcac ctttcttaca gaagggaccg aagccacatc
    35341 cataccgggt ttcactttcc agcggaaggt cttcatcgtc cacctccgga gaacaggcca
    35401 ccatcacgca tctgtgtctg aatttcatca cgggcaccct tgcgggccat gtcatacacc
    35461 gccttcagag cagccggacc tatctgcccg ttcgtgccgt cgttgttaat caccacatgg
    35521 ttattctgct caaacgtccc ggacgcctgc gaccggctgt ctgccatgct gcccggtgta
    35581 ccgacataac cgccggtggc atagccgcgc atcagccggt aaagattccc cacgccaatc
    35641 cggctggttg cctccttcgt gaagacaaac tcaccacggt gaacaatccc cgctggctca
    35701 tatttgccgc cggttcccgt aaatcctccg gttgcaaaat ggaatttcgc cgcagcggcc
    35761 tgaatggctg taccgcctga cgcggatgcg ccgccaccaa cagccccgcc aatggcgctg
    35821 ccgatactcc cgacaatccc caccattgcc tgcttaagca gaatttctgt catcatggac
    35881 agcacggaac gggtgaagct gcgccagttc tgctcactgc cggtcagcat cgccgccata
    35941 ttctgtgcaa taccatcaaa ggtctgcgtg gctgcacttt ttacctgcga catactgtcc
    36001 gtggcgctct cttcccactc actccagccg gacttcaggc ctgccatcca gttcccgcga
    36061 agctggtctt cagccgccca ggtctttttc tgctctgaca tgacgttatt cagcgccagc
    36121 ggattatcgc catactgttc cttcaggcgc tgttccgtgg cttcccgttc tgcctgccgg
    36181 tcagtcagcc cccggctttt cgcatcaatg gcggcccgtt ttgcccgttg ctgctgtgcg
    36241 aatttatccg cctgctgcgc cagcgcgttc aggcgctcct gatacgtaac cttgtcgcca
    36301 agtgcagcca gctggcgttt gtactccagc gtctcatctt tatgcgccag cagggatttc
    36361 tcctgtgcag acagctggcg acgttgcgcc gcctcctcca gtaccgcgaa ctgactctcc
    36421 gccttccaca aatcccggcg ctgctggctg attttctcat ttgctccggc atgcttctcc
    36481 agcgtccgga gttctgcctg aagcgtcagc agggcagcat gagcactgtc ttcctgacga
    36541 tcgcccgcag acaccttcac gctggactgt ttcggctttt tcagcgtcgc ttcataatcc
    36601 tttttcgccg ccgccatcag cgtgttgtaa tccgcctgca ggattttccc gtctttcagt
    36661 gccttgttca gttcttcctg acgggcggta tatttctcca gcggcgtctg cagccgttcg
    36721 taagccttct gcgcctcttc ggtatatttc agccgtgacg cttcggtatc gctctgctgc
    36781 tgcgcatttt tgtcctgttg agtctgctgc tcagccttct ttcgggcggc ttcaagcgca
    36841 agacgggcct tttcacgatc atcccagtaa cgcgcccgcg cttcatcgtt aacaaaataa
    36901 tcatccttgc gcagattcca gatgtcgtct gctttcttat acgcagcctc tgccttaatc
    36961 agcatctcct gcgcggtatc aggacgacca atatccagca ccgcatccca catggatttg
    37021 aatgcccgcg cagtcctgtc tgcccaggtc tccagcgtgc ccatgttctc tttcaggcgg
    37081 cgggtctggt catcaaaccc tttcgttgcg gcctcgttcg ccgcctgcaa tgccccggct
    37141 tcatcgccgg aacgctgcaa ctgagcaaca tacgcaatct gctccgccga cacgttatgg
    37201 aactggcgag ccatcgccgt cagccccgac gtcgggtctg tggtcagctt cccgaaggct
    37261 tcagcgacct tgtccacctc cacgccggat gcagaggaga aacgcgccac actctggctg
    37321 atggacgcaa tctgagcctc accgcttacc cccgccttaa ccagtgcgct gagtgactcg
    37381 ctggtctggt taaacgtcag ccctgccgcc tgcccggctc tggacaggac cagcatacga
    37441 tctgccgtca gtcccgcctg attgccggaa aggaccagcg ttttgttgaa atcggacagg
    37501 gttgagttgc cctgatacca ggcatacgcc agcgcaccgg tcgccaccgc cagcgaggtg
    37561 gcccccacca tcggcagggt gatcgcaccg gcaagccccc tgaacatggg gatcatcccg
    37621 ccgaaggagt ccttcacctg ccccccctgt tgcagcagga tcagccacgg actttgcccg
    37681 cctgcaagct gcgtggccac gtcggtgaac tgtgcaggca gcatacgcat ggcggcttta
    37741 tactgcccga cggaaatccc cgctttctgt gcagccagcg cctgtcggct cagcgactgt
    37801 tcaacgactg ccgctgtttt tttcgcatca ctttccgtac cagaaaaatg acgcctgact
    37861 ctggccatct gctcgtcaaa tctggccgca tccagactca aatcaacgac cagatcgcct
    37921 accggttcag ccataccgga ctcctcctgc gatcccttct gatactgtca tcagcattac
    37981 gtcatcctcc gtcatgtccg ccacatccgg ggaagcgggg ataacttcat tcccgtccgg
    38041 gccaaagcgg acacctccgg caagccctgc cgctttctgc atcagcacat catcttcagg
    38101 ctcttcgtca gcctcgcgcc ggttcagcag actgaaatcc agcggatgca tatccggatc
    38161 gctgaaaaac aggctgagca cggtgtacgt cagcccggaa aagtgcatat ccagcagaac
    38221 atcatgaaaa taatgggtac tgtaaaagcg gtgccagtcg gcatactccg tggatgacat
    38281 cccggcaagc atggcacgcc agtcgggtcg ccccatctca cgcgccagtt tcagggcaaa
    38341 actcagctca ccgtcgaaca ctttcccgca gaaacaggct ctgcgggccc ggcgtcctct
    38401 gtctgttcag gggcattatt caccacaaac tcatacatac cagacagccg gtacaccacg
    38461 ttttcagcat gagaaattgc ctccgtgggc caggtggtaa gcacttcctg ctcaatctgt
    38521 ttaacggctt cattcatgga cggcatctgc gtcttctgcg gatggttatg ccacagggac
    38581 atcgccacca gaaacgcgcc ggttctgatg gcgtcttcca cagtaaactt ccggttgctg
    38641 tctgactccg cctgttctgc ctgccgtttc atcagggcga gatgctcaat gcgctgcagg
    38701 gctgacagtt cagaaagcgt gacggtcaca ccgttatgtt caaatgattc ggttttcagg
    38761 aacatcgctg actctccgga ttaactggcg gtgacggtaa tttctgcaac cgcagcaaac
    38821 tcaccattac cggatacaac cggaatgttg accttgcctg cagcaacgcc gttcacggtg
    38881 atggtcatac cactgaccga cacggtggct tttgttttat ccgcagacac cgcacgaaag
    38941 ctcttgtcgg ttacgccctc cggctggaag gccacggtca gcgtggtgct ctgccctttc
    39001 accaccgagg tgctggcagg cgtcacggtc atgccggttg ccgctgttac cgtgctgcga
    39061 tcttctgcca tcgacggacg tcccacattg gtgactttca ccgtgcgggt gatcacttcc
    39121 ttcgccgtca ccgccttacc gatactgctg acccagccac ggaacacatc gaccgtgccg
    39181 ttcgggaagc ggattttata ggcacgggta tcgccttcat taaaccacgc cagcagcgcc
    39241 tgctgcccct gctctccggg catccacgcc agcgtgaagc tggtatctcc ggcagatttc
    39301 tgcccctgcc cggtcgcagt ccagtctgca tcttcatcat cgagatagct gtcgtcatag
    39361 gactcagcgg tcagttcgcc gggcgtcagg tctttaactt ttgccagacg cgaccagtca
    39421 acgtctgaaa gcggattcgc gtaagggtca ccgctcccct tataaaccca cagggtggtc
    39481 ccggcacctt tcaccggcat tgtaggattt ggtacaggca tagcgtcctc acatttcata
    39541 ggtaatgaca taagtcagat cggctgaact ccacaagccc gcatcatcgt cgcgccggta
    39601 gtcatagccg ctggccacca tactggtgat caaatctgac agtgccggga tatcgctcat
    39661 caccggataa atccgggact ccatccacgc atccagctct gaatccggca cctgagcagg
    39721 caggaaaact tcgatatgca gctccgcctg ccaggtatcg ctgtccagct cttcgcccgt
    39781 gtattcagcg ccggtgagat aaacggcaac tgccggaaaa tccgcctcat caaaaacagc
    39841 ggggcgacca tcaaaaaacg tcgccccggt gtcatgcttc tccagtgcat ccagtacggc
    39901 tgcacggagt tcagtatgtt tcatcgcttt attaccatcc tcagttgatg ctgcagcgca
    39961 tagcccagct ctttcggaag acgttcacgc cgtatccgct caatattttg tttaaacgcc
    40021 gtggtcagcg gcaccgccat cgggattttc accacatcaa tggggtaacg gtttttccca
    40081 gccacacgct gcatgacatg ccaccggcca tttttcagtt gctgaataaa cgcgccggga
    40141 atacgacggt tacccaccac aagcacgctg ccgccacctt tcagggatga acgctgcccc
    40201 tttttacgac gcctgcggcg cgaaaggaca acccgcgcat tacccagctt gattacgggc
    40261 aaatcccccc ggttaacttt gattctggcc tgcggatttt tgaccgtggc ccttttcagc
    40321 ctggcccttt cctttaccag tttccggcgt acctttgtct cacgggcaac ctgtgacgcc
    40381 gactgcgata tcgcggatga agcaacgcgg ttaatggcca ttgcggcggc accaggcacc
    40441 gccgttttgc tgatacggct gaggttttca acggcctgct caagaccttt tatggccata
    40501 catccccctt tcagcggcga cggttaacgg caggcggtac gccccgtcca agccagagat
    40561 gacaacttcc gccatcatcc ggcgaaaccc gatctaccca gaaattttcc tcaccgatgg
    40621 tcagcgtgtc tccacgccgc agctgccgca cctcatcagt ccggacaaac agggacgggc
    40681 tggagccttc aacgcgcacg ccctgtccgg catagctgat attttcaggg tcatcaaaaa
    40741 caccacgtat caccgcacct gactgctcac cggatgtaat ggtggctgac gttcccatgt
    40801 acccgcgtat cgtttcatcg gcgcgggcaa tggcagcatc gaacaggtta tcgaaatcag
    40861 ccacagcgcc tcccgttatt gcattctggc caggccgcgc tctgtcattt cggctgccac
    40921 accggcagag acacgaaacg ccgttcccgg cagcacaaat gccacaggtt catcccgcgt
    40981 ggcgtgaagt gcatcagtat gcagcttcac cagtgccacg accgtgacca gttcagacgt
    41041 atccagaatc acggtatccg gctgcgctga tcccacctca ttttcatgtc cggtcagcac
    41101 attttcccgg ctgagagggg tgtcctgacc ggcagtttca tccgtgtcat caagctcctc
    41161 tttcagctct gccacacgga gcgccagttc ttctttcgtc cccgtcaggc tgacatcacg
    41221 gttcagttgt tcacccagcg agcggagacg ggcaatcagt tcatctttcg tcatggactc
    41281 ctccacagag aaacaatggc cccgaagggc catgattacg ccagttgtac ggacacgaac
    41341 tcatcagggt cagccagcag catcagcggt gctgactgaa tcatggtgaa ctcacgcgcc
    41401 ggatcgccgg tggtcaccca gtttttcggg taacgggcag aggcgttaat gccttcgcgc
    41461 tgtgcgtccg catcctgaat gcagccatag gtgcgcagac cgcgtgcctg agtgttcccc
    41521 agcaccatcg tgttgtccgg caggaagttc tttttgacgc cgttttccac gtactgtccg
    41581 gaatacacga cgatggccac atcgccatac atccccttat aggacaccgc tttgcccagg
    41641 tctttcaccg ctgtctccag ctcggaatta gagccacgac gggtatccag cttctccttg
    41701 acggctttga aggaacggaa cagcgcccag cctttcggat cgaacacgat gatattcacc
    41761 acaccgctgg cgttcagcgc gtaggcttcg atatcgtcgg tcgggtcata cgtggacttg
    41821 tcacgcttgc tccactccgt gccgccggac tgcgtgatgt tattctcctc actgcggccc
    41881 atatccacct caaccggatc gaaggcttca ccggtcatgg tgtatttgcc cttaagcacg
    41941 gcagaaactg cctgcatctc ttcgacctga gcaatggcca gctcttcgtc acgcatgttc
    42001 tgcatgatga tgcgacggcg gcggtaagcc gggtccgcca gattctgcgg atcttcatcc
    42061 ggcaggcgac gcagggtcat ctgcggattc acttcatgct tcggcttgac atatcccggc
    42121 gtaaattcag aggtggagcc gccacgggaa cggataacct caccggaaac aatcggcgaa
    42181 acgtacagcg ccatgtttac cagtcccgga atttgtgaga gatagacttt ctccgtggtg
    42241 aagggatagc tctcacggaa aaagagacgc agaaacagcg gatcaaactt aaatttctgc
    42301 tcatttgccg ccagcagttg ggcggttgtg tacatcgaca taaaaaaatc ccgtaaaaaa
    42361 agccgcacag gcggccttta gtgatgaagg gtaaagttaa acgatgctga ttgccgttcc
    42421 ggcaaacgcg gtccgttttt tcgtctcgtc gctggcagcc tccggccaga gcacatcctc
    42481 ataacggaac gtgccggact tgtagaacgt cagcgtggtg ctggtctggt cagcagcaac
    42541 cgcaagaatg ccaacggcag caccgtcggt ggtgccatcc cacgcaacca gcttacggct
    42601 ggaggtgtcc agcatcagcg gggtcattgc aggcgctttc gcactcaatc cgccgggcgc
    42661 ggttgcggta tgagccgggt cactgttgcc ctgcggctgg taatgggtaa aggtttcttt
    42721 gctcgtcata aacatccctt acactggtgt gttcagcaaa tcgttaacgg catcagatgc
    42781 cgggttacct gcagccagcg gtgccggtgc cccctgcatc agacgatcca gcgcagtgtc
    42841 actgcgcgcc tgtgcactct gtggtgctgc ggccagaatg cggcgggccg ttttcacggt
    42901 cataccgggg gtttctgcca gcacgcgtgc ctgttcttcg cgtccgtgag cctcctcaca
    42961 gttgaggatc cccataatgc ggctgttttc tgccgcaacc gctgcggtga tctgcgcgtt
    43021 cacgtccggc tgcgccgcgc tggcgttctc gccctccgtc gctggcacca cgtcagtaac
    43081 gtcagcctgc gaagcagtgg ctgaaacagt tgttgattga gtctctttgg tcattcgccc
    43141 tcctgagaga cgggatttac gtgcatccag tgcatcacgc atgacggtga tcgcatcggt
    43201 gctgttaaca agttcatcag ccagtccggc atcaatggcc tcctgaccgc tgtacactgc
    43261 agcctcggta tccagcacaa cctgcacgga caggccggta tatgccgaca ccttctgcgc
    43321 aaacatctgg cgggttgcgt ccatccggga ctgcagtgtc tcccggacgt catccggaag
    43381 atggctgtag gggttgccat ccaccttatg gctgccgctg taaatcagcg tgatttccac
    43441 accctgtttc tccagcgcag caccgtaatt actgtgagcc atcatgacgc cgatggagcc
    43501 tgtccgggcg gtctgcgtga ccagacgccg ggaggcggca ctggcaagca actgacctgc
    43561 actgcagttc atgtcgttgg caagcgccca taccggtttt atgtcacgca cacgggcgat
    43621 gatgtcagcg cagtcaaatg cccccgccac catcccgccg ggcgtgtcca tatcgagcag
    43681 aatgccgtcc accatcggat cgctggcagc ctgttgcaga cgggcgataa tgccgttgta
    43741 accggtcatc cccgagtacg gctgcagcgc ccgcgtccgg ctgaccagcg tgccggacac
    43801 cggcagcacg gcgatgccgt tcatgacctg ataactgcgg gcctgtcgtg gtccgtcatc
    43861 atcaccggat aatgccagcg tcgcgagtgc ctcctgggca gtcaggctgt cgccggacac
    43921 cgcatccgtc aggctgctga tcccaagctg gcctgcaagc gcacaaaaga aaacccgcgc
    43981 ataggcgggt tcaagcatca gcggctcatt aaaggccatg ctggcaatat gcgggagatt
    44041 acgcagctct gctgtcactc ttctcctcct ctgttgattg tcgcagcccg gattcaaatg
    44101 ctgcagccgc ccaggcgggc ggtttaagac cggctgcacg gcgctccatc gtttcacgga
    44161 cctgctgggc aaaaatttcc tgatagtcgt caccgcgttt tgcgcactct ttctcgtagg
    44221 tactcagtcc ggcttctatc agcatcaccg cttcctgaac ttctttcaga ccatcgatgg
    44281 ccatacgacc ggagcctatc cagtcgcagt tcccccaggc actgcgggct tcctgaaaac
    44341 tgaagcgcgc ttttgaaggt aacgtcacca cgcggcgaac gatggcctct tccagccagc
    44401 acagaaacat ctggctcgcc tgacgggatg cgacgaattt tcgccgcccc ataaagtacg
    44461 cccacgactc gttcgcactg gcccgtgccg tggagtagct catctgggcg taattccggg
    44521 aaagctgctc atacgagaca cccagcccgg cagcgatata ccgcagcagt gactgctcaa
    44581 acacggagta gccgttatcc gtatcctgag ccgtctgcag gttcagtgag tcacccggca
    44641 tcaggtgcgg tacttttgcg cctcccagcc ggaccggcgc tgcggcgtaa tacgcggcaa
    44701 tttcaccaat ccagccggtc agcctttccc gctgctcctg actgttcgcg cccagaataa
    44761 aatccatcgc tgactgcgta tccagctcac tctcaatggt ggcggcatac atcgccttca
    44821 caatggcgct ctgcagctgc gtgttctgca gcgtgtcgag catcttcatc tgctccatca
    44881 cgctgtaaaa cacatttgca ccgcgagtct gcccgtcctc cacgggttca aaaacgtgaa
    44941 tgaacgaggc gcgcccgccg ggtaactcac ggggtatcca tgtccatttc tgcggcatcc
    45001 agccaggata cccgtcctcg ctgacgtaat atcccagcgc cgcaccgctg tcattaatct
    45061 gcacaccggc acggcagttc cggctgtcgc cggtattgtt cgggttgctg atgcgcttcg
    45121 ggctgaccat ccggaactgt gtccggaaaa gccgcgacga actggtatcc caggtggcct
    45181 gaacgaacag ttcaccgtta aaggcgtgca tggccacacc ttcccgaatc atcatggtaa
    45241 acgtgcgttt tcgctcaacg tcaatgcagc agcagtcatc ctcggcaaac tctttccatg
    45301 ccgcttcaac ctcgcgggaa aaggcacggg cttcttcctc cccgatgccc agatagcgcc
    45361 agcttgggcg atgactgagc cggaaaaaag acccgacgat atgatcctga tgcagctgga
    45421 tggcgttggc ggcatagccg ttattgcgta ccagatcgtc tgcgcgggca ttgccacggg
    45481 taaagttggg caacagggct gcatccacac tttcactcgg tgggttccac gaccgcaact
    45541 gccctccaaa tccgctgcca ccgccgtgat aaccggcata ttcgcgcagc gatgtcatgc
    45601 cgtccggccc cagaagggtg ggaatggtgg gcgttttcat acataaaatc ctgcaggtcc
    45661 cctgcgtcgc tgtgtcatgc cggtctgcac ttccagctct gcaatatatt ttttcaggtc
    45721 agacacggaa gtggccgtaa actccaccct tcgtccgtct ttctgtactg ttgccacccg
    45781 tttacctgtc atcaggtcat gcagtgccgc acgggcagcg gcaagttctt cctgtcgcgt
    45841 cattcatcct ctccggataa ggcacgggcg taatctgcca gtgttttctt gttggttgct
    45901 gcaccatcct cttcctgcag gctcgccagc agcgcactga gatccagctg ccagcgggaa
    45961 atactgatgc gcagcgccgc cagcgcataa acgaagcagt cgagtgcctc attgcgtcgc
    46021 tttttgctgt cccacagtat ttttttcctg ccatccaccc atttttcgac ctgctcttca
    46081 gcagtcagct gctgcgcttc ggtcagatca aaaatatccg ggttattcgg gaagtgaacg
    46141 gcaccgggaa gcggttcatc cccttccggc gtcagtgtga agcggttata aatctgctct
    46201 ttcgcggtat ccgtaccgat ttcggtaagg taaaccccgt ttttgtttcg cttacgtggc
    46261 atgctggcca ccggctttcc gtagacggat gcccctttaa tggggatcac ccggaacagc
    46321 ccatgttttt tcgagcgttc atacacaatg gtcgggtcaa tcccgccagt atcccagcag
    46381 atacgggata tcgacatttc tgcaccattc cggcgggtat aggttttatt gatggcctca
    46441 tccacacgca gcagcgtctg ttcatcgtcg tggcggccca taataatctg ccggtcaatc
    46501 agccagcttt cctcacccgg cccccatccc catacgcgca tttcgtagcg gtccagctgg
    46561 gagtcgatac cggcggtcag gtaagccaca cggtcaggaa cgggcgctga ataatgctct
    46621 ttccgctctg ccatcacttc agcatccgga cgttcgccaa ttttcgcctc ccacgtctca
    46681 ccgagcgtgg tgtttacgaa ggttttacgt tttcccgtat cccctttcgt tttcatccag
    46741 tctttgacaa tctgcaccca ggtggtgaac gggctgtacg ctgtccagat gtgaaaggtc
    46801 acactgtcag gtggctcaat ctcttcaccg gatgacgaaa accagagaat gccatcacgg
    46861 gtccagatcc cggtcttttc gcagatataa cgggcatcag taaagtccag ctcctgctgg
    46921 cggatgacgc aggcattatg ctcgcagaga taaaacacgc tggaggggtc atccggcgtc
    46981 catttgaggc caaacggcgt ctctttgtcg ccaaatttaa gatactgctc ctccccgcaa
    47041 tgcgggcagg caacatgaaa acgcataaaa tgcggggatt cactggctgc acgctcaatc
    47101 tgacaggtgc ctctcacttt tggcgtggag ccacggatgg actttggcca gaccgagcct
    47161 tcaatacgct tgtcacccag gaacgtcgga gagccttcct gttcaatatc atcatcaaaa
    47221 gcagcaagtt catcataacc cgccacatcc accgactttt cacggtagtt ttttgccgct
    47281 ttaccgccca ggcaccagaa gccacgccca ttagtgaaac gcttcatggt gagcgtgtta
    47341 tcccggtgct ttttgccata ccacggggcc agcgccagca gcgacggaat atcacgaata
    47401 gtcggctcaa cgtgggtttt cataaagttc tcggcatcac catccgtcgg caaccagata
    47461 agggtgttgc gctgcttatg ctctataaag taggcataaa cacccagcag cattttggaa
    47521 taaccgacac gggcagactt caccacattc acctcacgga tgtagtcgct gcccatcgca
    47581 ttcatgatgg cccgctgaaa gggcagtgtt tcccagcgcc cttcctggta tgcggattct
    47641 ttcgggagat agtaattagc atccgcccat tcaacggcgg tctgtggctc cggcctgaac
    47701 agtgagcgaa gcccggcgcg gacaaaatgc cgcagcctgt taacctgact gttcgatata
    47761 ttcactcagc aaccccggta tcagttcatc cagcgcggct gctttgttca tggctttgat
    47821 gatatcccgt ttcaggaaat caacatgtcg gttttccagt tccggaaaac gccgctgcac
    47881 cgacaggggg agcccgtcga gaatactggc aatttcacct gcgatccgcg acagcacgaa
    47941 agtacagaat gcggtttcca ccacttcagc ggagtctctg gcattcttca gttcctgtgc
    48001 gtcggcctgc gcacgcgtaa gtcgatggcg ttcgtactca atagttcctg gctggagatc
    48061 tgcctcgctg gcctgccgca gttcttcaac ctcccggcgc agcttttcgt tctcaatttc
    48121 agcatccctt tcggcatacc attttatgac ggcggcagag tcataaagca cctcattacc
    48181 cttgccaccg cctcgcagaa cgggcattcc ctgttcctgc cagttctgaa tggtacggat
    48241 actcgcaccg aaaatgtcag ccagctgctt tttgttgact tccattgttc attccacgga
    48301 caaaaacaga gaaaggaaac gacagaggcc aaaaagctcg ctttcagcac ctgtcgtttc
    48361 ctttcttttc agagggtatt ttaaataaaa acattaagt
//
LOCUS       DASJTM010000009        48073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJTM010000009 DASJTM010000000
VERSION     DASJTM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.009"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            163..1323
                     /locus_tag="U4B29_000494"
     CDS             163..1323
                     /locus_tag="U4B29_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2105464.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1339..1782
                     /locus_tag="U4B29_000495"
     CDS             1339..1782
                     /locus_tag="U4B29_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105465.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(2265..2795)
                     /locus_tag="U4B29_000496"
     CDS             complement(2265..2795)
                     /locus_tag="U4B29_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN2105466.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            2900..3451
                     /locus_tag="U4B29_000497"
     CDS             2900..3451
                     /locus_tag="U4B29_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2105467.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(3604..4077)
                     /locus_tag="U4B29_000498"
     CDS             complement(3604..4077)
                     /locus_tag="U4B29_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2105468.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            4215..5687
                     /locus_tag="U4B29_000499"
     CDS             4215..5687
                     /locus_tag="U4B29_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2105469.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            5684..6394
                     /locus_tag="U4B29_000500"
     CDS             5684..6394
                     /locus_tag="U4B29_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN2105470.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            6746..7030
                     /locus_tag="U4B29_000501"
     CDS             6746..7030
                     /locus_tag="U4B29_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN2105471.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(7311..7730)
                     /locus_tag="U4B29_000502"
     CDS             complement(7311..7730)
                     /locus_tag="U4B29_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN2105472.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(7732..8316)
                     /locus_tag="U4B29_000503"
     CDS             complement(7732..8316)
                     /locus_tag="U4B29_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN2105473.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(8363..9838)
                     /locus_tag="U4B29_000504"
     CDS             complement(8363..9838)
                     /locus_tag="U4B29_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN2105474.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            10019..10330
                     /locus_tag="U4B29_000505"
     CDS             10019..10330
                     /locus_tag="U4B29_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105475.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(10445..12781)
                     /locus_tag="U4B29_000506"
     CDS             complement(10445..12781)
                     /locus_tag="U4B29_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2105476.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            12923..13591
                     /locus_tag="U4B29_000507"
     CDS             12923..13591
                     /locus_tag="U4B29_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2105477.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            13594..14637
                     /locus_tag="U4B29_000508"
     CDS             13594..14637
                     /locus_tag="U4B29_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2105478.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(14874..15755)
                     /locus_tag="U4B29_000509"
     CDS             complement(14874..15755)
                     /locus_tag="U4B29_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2105479.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            15914..16642
                     /locus_tag="U4B29_000510"
     CDS             15914..16642
                     /locus_tag="U4B29_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN2105480.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            16798..17115
                     /locus_tag="U4B29_000511"
     CDS             16798..17115
                     /locus_tag="U4B29_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2105481.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            17108..17716
                     /locus_tag="U4B29_000512"
     CDS             17108..17716
                     /locus_tag="U4B29_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN2105482.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            17771..18562
                     /locus_tag="U4B29_000513"
     CDS             17771..18562
                     /locus_tag="U4B29_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN2105483.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            18700..19188
                     /locus_tag="U4B29_000514"
     CDS             18700..19188
                     /locus_tag="U4B29_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2105484.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            19239..20111
                     /locus_tag="U4B29_000515"
     CDS             19239..20111
                     /locus_tag="U4B29_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2105485.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            20287..20649
                     /locus_tag="U4B29_000516"
     CDS             20287..20649
                     /locus_tag="U4B29_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2105486.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            20658..21410
                     /locus_tag="U4B29_000517"
     CDS             20658..21410
                     /locus_tag="U4B29_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105487.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            21424..23226
                     /locus_tag="U4B29_000518"
     CDS             21424..23226
                     /locus_tag="U4B29_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105488.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            23503..24543
                     /locus_tag="U4B29_000519"
     CDS             23503..24543
                     /locus_tag="U4B29_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2105489.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24595..26739)
                     /locus_tag="U4B29_000520"
     CDS             complement(24595..26739)
                     /locus_tag="U4B29_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2105490.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(27096..28469)
                     /locus_tag="U4B29_000521"
     CDS             complement(27096..28469)
                     /locus_tag="U4B29_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2105491.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(28469..28864)
                     /gene="rbsD"
                     /locus_tag="U4B29_000522"
     CDS             complement(28469..28864)
                     /gene="rbsD"
                     /locus_tag="U4B29_000522"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN2105492.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(28892..29809)
                     /gene="rbsK"
                     /locus_tag="U4B29_000523"
     CDS             complement(28892..29809)
                     /gene="rbsK"
                     /locus_tag="U4B29_000523"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN2105493.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(29917..30930)
                     /locus_tag="U4B29_000524"
     CDS             complement(29917..30930)
                     /locus_tag="U4B29_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2105494.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(31011..31610)
                     /locus_tag="U4B29_000525"
     CDS             complement(31011..31610)
                     /locus_tag="U4B29_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2105495.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            31796..32497
                     /locus_tag="U4B29_000526"
     CDS             31796..32497
                     /locus_tag="U4B29_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105496.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            32507..35218
                     /locus_tag="U4B29_000527"
     CDS             32507..35218
                     /locus_tag="U4B29_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105497.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            35669..37297
                     /gene="treC"
                     /locus_tag="U4B29_000528"
     CDS             35669..37297
                     /gene="treC"
                     /locus_tag="U4B29_000528"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN2105498.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(37357..38538)
                     /locus_tag="U4B29_000529"
     CDS             complement(37357..38538)
                     /locus_tag="U4B29_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2105499.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            38790..40313
                     /locus_tag="U4B29_000530"
     CDS             38790..40313
                     /locus_tag="U4B29_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2105500.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(40382..41110)
                     /locus_tag="U4B29_000531"
     CDS             complement(40382..41110)
                     /locus_tag="U4B29_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2105501.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(41123..42403)
                     /locus_tag="U4B29_000532"
     CDS             complement(41123..42403)
                     /locus_tag="U4B29_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2105502.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(42544..43146)
                     /locus_tag="U4B29_000533"
     CDS             complement(42544..43146)
                     /locus_tag="U4B29_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2105503.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            43391..43597
                     /locus_tag="U4B29_000534"
     CDS             43391..43597
                     /locus_tag="U4B29_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105504.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            43909..44628
                     /locus_tag="U4B29_000535"
     CDS             43909..44628
                     /locus_tag="U4B29_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2105505.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(44709..45392)
                     /locus_tag="U4B29_000536"
     CDS             complement(44709..45392)
                     /locus_tag="U4B29_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN2105506.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            45610..47796
                     /locus_tag="U4B29_000537"
     CDS             45610..47796
                     /locus_tag="U4B29_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN2105507.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        15599 a         8506 c         9594 g        14374 t
ORIGIN      
        1 agcccaaccc aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg
       61 caggacagct ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata
      121 taataaaaga aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa
      181 aaacagtctc gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta
      241 ctgatcgctt tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt
      301 atcgaacaag tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag
      361 gagaaaaacc aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa
      421 aagaacagaa atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc
      481 aatataccta agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt
      541 gaaaatggag caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat
      601 tcagtgatct ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt
      661 ttagaaaacg gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt
      721 aaaaatatac aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa
      781 gacttagtta ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg
      841 acaggacatc ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag
      901 gaaagaacca tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg
      961 ttagtgttta tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt
     1021 tcatttatta ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa
     1081 cataagaaaa aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat
     1141 gggaaagtca agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat
     1201 cctaaagttg ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt
     1261 gaggggcgaa aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa
     1321 taagtaagga gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa
     1381 tctgttctat cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact
     1441 tattgggaga atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta
     1501 gcagaaatag atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga
     1561 atctttgtga aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata
     1621 aaagaaacca ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat
     1681 ttctatcttt cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag
     1741 gaggaacttg tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat
     1801 aatgctccca gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa
     1861 ctgtgtgaat atgcagttgt tacaatcttt gataaacggt gttcaatcag ctgaccctcg
     1921 gtattagctc aaaaaacggc aaaatatgag gaactatttt tccatttgtt tctctaatac
     1981 tcaggtcaaa acgctgattt cacaacctct tgataaacgg tgttcaaacg gctgcttctc
     2041 ggtaataagc ccactagcct agaaaatatg aaaagctatt ttctaactag cgttcttatt
     2101 actcaaagca taacgctgat ttcacaacct cttgataaac ggtgttcaat cagctgaccc
     2161 tcggtattag ctcaaaaaac ggcaaaacat gaggaactat ttttccgttt ctttctctaa
     2221 tactcaggtc agaacgctga tttcacaacc tctctttcac aaccttattt tactcgttta
     2281 taattttcat cgaagtattt tccgctacgg atatcatctg gcattccatc gtatggttct
     2341 tcgctaataa catcttcatt gtctatacct gcatcaccca tataagtgat cgtagcaaat
     2401 tcaggaacaa gtaattttgg ataagtttca tatttttcac gagctgcttc catttctgcg
     2461 atatgttcgt tttggaaagt aacagtgatt tctggatgtt cttcaaccca tttagggaaa
     2521 aagatcattc cgtgaagttt cttttcattt ggcataaagt ccaatgccac gtctgtccct
     2581 gttggctctg tccaagtgat tttgaatacg ccgtccgtca atttaacgat atcagcttct
     2641 tggtctttta cccaacggcc tttgaccatt ccgccatgga tacggtaatc aacagtatgg
     2701 tcatttttgg cataccattc atattcccaa ccattgtcat acgtatagat aaagtgagtt
     2761 ccaataaaat cttccaattc tttaaattct ttcatgttta agcatctcct tataaattaa
     2821 aacatgtaat aaactacatg ttttcgatta ctcatttatg atagaatgga tttaaagatt
     2881 tgtcaaggag ggaaaactga tggcaagaaa aaatacactt cgatatattt tgttagggtt
     2941 attaagtaag aagaagatga caggatatga attgaatcaa tcgttcaaaa atgaaatagg
     3001 agaattttgg caagcaaaac atagtcagat ttatcctgaa cttgcaaaaa tggaagagca
     3061 aggaatcatt cagcatcaag tagagatcac aggtgaaaaa ttggaaaaga aagtttatga
     3121 attaacagaa gaagggaagg aacaattggc agagtggatt catacaccca ccaatgaact
     3181 accggttaat cgagacgaat ttgttctgaa attgtatttc gtcaaagacg tgaatgatcc
     3241 ggcattagta gaaattattc agcagcagcg tgaccttcat gaagaaaaac gacttcatct
     3301 attagagcga aaaaaaacga tctttcctac tgaaaaagaa aaacatgaaa attacggaca
     3361 ctacctgatt ttagcgcatg cgatcaatcg ggaaacagaa tatgcaaatt ggctaacaga
     3421 tgtattagaa gaaagtaacc gaaaaaaata aaaaaatatc cgaacagtga tggagaattc
     3481 ctttctatat gaaagaaacg cagatcaccg ttcggatatt cttatgttca aacaggcctt
     3541 ttttcaagta tttatgtagc atctacttat taaaaagctt ggtacttagg agattagtgg
     3601 cgtttagagt tcttttaaca actgttcgta gtcagctttt tcatcgacag gaaggagctt
     3661 gtaagttaat tcgtctgtct cgttgtcatg aatgatgtac atcttagcat tctcttcttt
     3721 gaagttaccg atttgtaaaa tcgtatagcg ttcgtcgatc aattcgatat ctttgattgc
     3781 ttttgaaaaa actttacgga catggtgcat tttcttttgg cggatttctg ccttttctgc
     3841 agcttttgac aaagtaaatc cgtcatctaa atattttttg atcagttcta cttttatgac
     3901 tgtaggtaaa cgatattttc taggacaatt cgcatcagct tcgatcgaac gaatgaatcc
     3961 cttttgttcc caatacctta attgacgtgg agacacacct acgatatcac ttaattcact
     4021 gatgccaaca accaattgat cattttccaa caaacgttta agggaagccc caaccatttt
     4081 tgtcactcct tttgtcctct tgcttccctt aattatatcc gtattaactt atgtgtcaag
     4141 taggttgaca aatagcgaat ttaggactaa agtattctag tagtttcaga aaaaattcag
     4201 aaagaaggag aaatatgagt aacaaccaac ccgtagatat ttacggaaaa ccatataatc
     4261 ggacactttt agttgttgtc ttattaattg gtacattttg taccgttttg aatcagacct
     4321 tattgacgac agcttttcct acattgatga aagctttcga tatttcagcg tctgatgtgc
     4381 aatggttaac gactggtttc ttgctagtca acgggatcat gatcccaata acagcatggc
     4441 tgatcaataa attcagttca agaaatttat atctttctgc catgacgatc tttttgatcg
     4501 gaacgattac ttgctttaca gcacctaatt ttagtacatt actaatcggt cgtttgatcc
     4561 aagcttgcgg tgtgggagta tcgatgccat tattgcaaaa tatcatgcta tctattttcc
     4621 cgccagaaaa aagaggatca gcaatgggga tgtcagggat cgttattgga cttgctcctg
     4681 ctttaggccc tacattatct ggttatatca ttgataacta ccattggcgt gacttgtttg
     4741 gtatggtact gccaatcgtt gttcttgtct tggttttagc attcttccta atgaaaagtg
     4801 ttattccatt gtcaaatcct agcattgata ttttatccgc tattttatca acgattggat
     4861 tcggtagttt actttacgga ttttctagtg taggagatga cggctgggga agcttgaaag
     4921 taattggttt cttagttgtt ggtgtgattt tcatcgcctt gttctcatgg cgtcaattac
     4981 atttggaaca tccattcttg gaacttcgtg tattcaaatc atctacattc acgattgcag
     5041 ctgttttagc tggtgtaaca aatatggcga tggtaggggc agaaatggtc gtaccattgt
     5101 atattcaaaa tattcgtgga gaatcagcat tccattctgg cttgatgttg ttgccgggtg
     5161 cattgatcat gggggctatg atgcctgtga cgggtgcgat tttcgataaa tacggcgcga
     5221 aacgtttagc catcacaggg atgttcattt tgacagcggc aactgcacca tttgctttct
     5281 tgacaaaaac gactccagtt ttatatatcg tgatccttta tgcgatccgt atgtttggga
     5341 tctccatggt catgatgccg gtaacgactt ctggtatgaa tgcattgcca aataacttga
     5401 tcagtcatgg gacagcggta aataatacat tccgtcaaat cgcaagctcg attggtacag
     5461 ctattttgat cagtgtgttg acgaatgtga caaaagcaaa tatcccaagc aaatctgtat
     5521 tggataatac gccattagct tataaagatc aggcaatcaa tgccacactg tcaggttacc
     5581 atgccgcgtt tatcgtagcg atcatctttg ggattcttgg ctttattatt gcctttttat
     5641 tgaagaaaaa agaacctgtt ctaatgaaag cgggggataa tgcatgatta ttggagtttt
     5701 gattgtcagc ttgcttgctt ttgctttaac gaacgtgttc gctaaaaaaa catggcaaac
     5761 ctttttatct ttgattttcg gactaatctt cgtcgcttca ctaagtttga tcgtcgctaa
     5821 tttgtccaat cactttggaa tggaaaaagt aacggaaaca aaaacagaaa aaattgtttc
     5881 cagtgctgat tcccaaggag cagatatgct tttgtataaa gctttaggga atggaaaaga
     5941 aaaagtgtat ctttaccgaa caaatgaaaa acaagaaaaa ccaaaagcaa ctggtacgga
     6001 taacgaaaca aataaagtag aaaagacaga cggcgatgct gaaaaagtga cgaaaactac
     6061 ttattgggaa tacaaaaacg acatgtataa attctggttc aatattgctg ataacaatca
     6121 cgaatatgac agccgagtga atacattcaa aatcccagag acatgggtag aattaagtac
     6181 ggaccaagca gccaagttag ctgaattagt gaagaaacag caaagtacga tggaaagtga
     6241 agcaaaagct tatgttcaag acggtatggt aaaagcaatg actgaaaatc caaaaatgag
     6301 caaagcagag caagaacaac gtacgaaagc cttagctgct gaattccaac aacaagcatt
     6361 cgcaaaatta gtgaaagaag caaaaggcga ataatatttt gaacaccatg taggaggttg
     6421 tgaaaaagct gctctgcatc aagcagtaag aacaatcaga taaaaatagc actccatatt
     6481 tttcatctga ttgggcttag tgccgtagat gcaagctttt gaacaccgtt taaggaagag
     6541 gttgtgagcc tgccgtttaa ctctgagtca caaggagcaa tttcaaaaaa cagcttctca
     6601 tgtttgttga ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcga
     6661 aaaaaggaac tgcgacaaga cttttgtcac agttccttct tttttttgct atgctatact
     6721 agatgtaaaa aataggagaa ataaaatgag ggaaatttat tcttttgaat caaaaatcta
     6781 tgcttccgaa gtcggcaagg gcggggcata tattattttt ccgtatgata taagaaaaga
     6841 attcggtgct ggaagagtta aagtccaagc aacttttgac aatgtgccgt atgaaggcag
     6901 tatcgtgaat atgggcgtga aaaatccaga tggttcagtc tgctacatcc ttggactacg
     6961 aaaagatatt cgtaaaaaca tcggaaaaga aatcggtgat attgtagcag ttacagtgaa
     7021 gcaaaaataa aagaggttgt gacaaaagac gtgtcacaac ctcttgataa acggtgttca
     7081 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaacc catgtttaca
     7141 aaatttttct tactactcga aacgaagcgg ctttatcaca acctcttgat aaacggcgtt
     7201 caatcagctg tccctcggta ttagctcaaa aacggcaaaa tatgagaagc tatttttcca
     7261 tttttttctc tcatactcag gtcataacgc tgatttcaca acctcttctt tcataatggt
     7321 ttttcaccat ctttataata aactggttct ttgatatgga aatctatttt ttccaaccaa
     7381 tctaaaatct tgtaacctaa ctcttctgac atcggaaaag aggcgttgat ttgtgcattg
     7441 gagatatggt caacatggat accggaagtg atcgtacaac ttcccggaac ggcagttttg
     7501 atgatcacag ctattttttc ggctaagaca tcgtctttat ggaaccgacc atcgtggcta
     7561 gggaaacgta cagtttgcat ctctgtatca cgagaaaatg tagtgactgt tccgatatgt
     7621 ggtgtatctc cgcctgttaa agtgatcaat aagtctttac cgattacttt aacagcagct
     7681 tggatcgtga aacctagttt ggttacttca actatcgttt ctttttccat attaatcacc
     7741 ctgccaacgt tttgaatgct catttacaat ttcaaattga tccaaaagtt tcattgtatg
     7801 gtgattaaca agatcttgga tcgtttgagg ctgattataa aaagcaggaa ttggtggtat
     7861 gatttgcaca cctagtcgtg ctaatttagt caagttttcc aagtgaatgg catttaatgg
     7921 cgtttcacga ggaacaatga ctaactttcg ttgctccttc attgttacat cagcagcacg
     7981 tgcaatcagg ttgtcgccta aaccaacaga gatacctgcg actgttttca tacttgccgg
     8041 tacgatgatc attccatcgg tcaaaaatga tccgctggcg acagcagcac ctaaatcttt
     8101 gtttgaataa tggtaatcaa acaggctttc gaattcagtt agtgataagt cggattccag
     8161 ctttaagttc tgttttgccc atggactgat gattccgtgt gtttctatca tcggatattc
     8221 ttttagtttc ttcactaggt tgatggcata aatggtgcct gaagcgccac ttacaccaat
     8281 cacgattttt ttcttccgtt gtgccaattg ttccattttc cgcactcctt tttatgatgt
     8341 caaaacagtt gcagatcatt cattatttca aatatttttc ccaatcttct acttcttgga
     8401 attttgcacg tacaaaatgt tctttcatat cgaaaggaac tgtaccgtca aagatagttt
     8461 ttgcacttat cccacggaaa cgaatagagc caggattata ttctggtaat tctgatgggt
     8521 ccaatggatg gttcctcatt cctgggatca cgatgatatc tttatctcct tggaaacgag
     8581 tgttgaaagt ccaaactaca tcgttcatgt cgaaaatatc aacatcttca tcgactaaaa
     8641 tgactgtctt caattctttg aaggctgata aagcaagaat agctgcttga cgctgaatac
     8701 cttcatccgc ttcgttttct ttacggattt gcatgatggt catcaattta ccaccacctg
     8761 caggaggatt gtagacattt actacttttc ctggaatagc tttatcgacc aaggcaagaa
     8821 tacttgcttc tgtaggaata ccggccatag atacgtgttc ttcactggga ccaatcgttg
     8881 tttgcatgat tggattgtct tttcggtgag taactgcttt gactttcact acttgtacag
     8941 ctggattagc atccccagta tagccaggaa attctggcat tgcttttcct gagttcgtgt
     9001 tgatatcttc ttgcattgtt tcatttggca tgatttctgc ttcaatgatg aattcagcac
     9061 gagcaatacc cacttcatcg actgctatac catcaacgat ttgaataggt tcgttacgca
     9121 aagctcctgc acaccatagt tcattgtagc caagtggagt ggtaggtggt tcgaaagtcg
     9181 tgccaatggc aattgctgga tctaaaccaa tattgatagt gataggcatt ggtttattca
     9241 aggcttcgaa ttgtttttgg aagtgaccaa tgtggcgtcc gccaggcatg atgtacatac
     9301 cgatcgtatc tttatcttct agaaccatac gatggatcgt tacatcgctc attgtttttt
     9361 ctggatctga tcctaagact acacccattg tgatgtatgg accagcatct tcaggagtat
     9421 ttgtaggtgc aggcaaaatg ttgcgaatat cgaagtctgc atcggtagct agatgaacga
     9481 cttcttgtgc tggagcgtct gctttgtcta ctttcacagg tttgattgga ttttctacag
     9541 cgtctttcaa gaaacgacca agtgttcgat aatcatggtg caaaatttca cctacacgtt
     9601 tacgactagc cattgttccg atgttcacac gtgtgccggg gaaacctttg atattgttga
     9661 aagtcatagc tggtccttct tgagtcggac gttttactgt accgccagca ccgatataac
     9721 ggtaaacacc agataattct gcttcaggat cgatttcaat atctgtttcg tgatattgac
     9781 ctggaagtgt ttttagttct tcgatgactt ttcttaaatc atacggttga tctgtcatga
     9841 gagattcctc cttagttgtc tttacctatc tatagtaaaa ggtgaaaaag aggaaaacaa
     9901 ttcaacttgt tcggtaaagt attctacaat ggaatggatg cgtttttttg ataatagaat
     9961 aaactatgat atacttatca ctaaaagaaa gcgcatactt agtgttggag gaaggaaagt
    10021 gaaaaaaatg gaggagtcat ttcctaaagc agtaaaagtg gaaaatatag cgaatatttt
    10081 gaaagttaca tttgaaaacg gagaagtaaa atacgtgaaa tctcattgga cagaagagat
    10141 aaccgatgcc ttgcaatttg gaaaaaaagg aagaggaaaa agaaaaaatc ttctcgcact
    10201 ttctcgtaat atgtggattg ggactgaagt aacgattgaa gcagatggaa cagtattcat
    10261 aaatggaaaa gacaggtata cgccagaaga gttgtggtac aaaggaaaga aaagtatacc
    10321 agaattataa aaaataatta aaaaaaacag cgcgaaacaa ggaacatatt acttcctcgt
    10381 ttcttgctgt tttctactat ttcagcgctc ttatgactca aatctaaatg tggttgtcac
    10441 aaccttattc tgcacgcaaa gcgatagcgg cgtctttttt cgctgccatt cgtgcaggaa
    10501 gatgaccgcc aagcatcgtc aatatagtag agacgatcac tagaataatc gcatggacag
    10561 gatttagttg agcaacattt tttagatccg tcatgttgta taagatgcta ttgataggga
    10621 acgtagctag ccatgcaata aacacaccta atatcccaga agaaatacct aaaatacacg
    10681 tttctgcgtc aaatacgcga gtgatgtctt ttttacgtgc acctaaagct tttagtacac
    10741 cgatttcttt tgtccgttca atcacagatg tataagtaat gatactgatc atgatcatac
    10801 ttgttactaa agagatccct gcaaaagcaa taaggacata agtgatagca tccatcaatc
    10861 cgccagtcag ttcagtcatc gttccagcta aatcagtata aatgatttta tcttcatctg
    10921 atttgccttt attgtaatca tctagataat ccaagatctt ttctttgtct tcaaaattgt
    10981 tcggataaat catgatactt gaaggcaagc tgtctccacc taaataacta agcaatgttt
    11041 gtttggcatt ttcgtcaact ttttcagtag ttaagacatt tacaccactg tctctttgag
    11101 ctttgacgat ttctgattct ttgttttcgt tgacgatttg agtcgttaat tgatcgctat
    11161 aagcgattcc tggagataag agattcattg tagaagaaga tttcacgcgt aggatccccg
    11221 agatcttcaa ttcatcagaa gcattctgat acatcgcatc ataatctgta ttagggataa
    11281 aattcccagt tggaagtttt gtatagtaag tattgttgtt tactaatttg aatgttgtac
    11341 caacgatatc atcaaaatca agtttttcgt cttctttcac atcaaatcca agattcttca
    11401 atgcgttgat attagtatta ttattgccgt ctacgatcag cacgacatct gttgctgagg
    11461 ctggataact tccggcaagt aaagaataat tgtcttttaa aaagttttct ttgctagtgt
    11521 caagctgtgt agggaaactt gacactccca cacctgtcat tgctgacatc gtactagata
    11581 aagaaagaga ttccgtatca ggattttgat tcgagaagct gacaggctgt actttgccat
    11641 ttacatcacg taaaagattg attcctgtag tacgggtaaa tccgatatta ttacttaagt
    11701 taggatcgat gtctgttacg taatcgacgt atgtttgatc aatcttattc gtgtgctgtg
    11761 ctttatcttc ttcgctgact tttgcagtaa tcgttttgct gtctggataa tccgctttgc
    11821 ttgcacccaa tccggcatcg tcacgagtct ggttagtcgt taccttagaa atagtaatcg
    11881 gaaatttcgc catcgtttca gcttgcgtgt tatcgatttg tttttggaat ccagaagaaa
    11941 gggacagaac gatcgcgatt ccgatgatcc caatactgga agcaaaggag gtcaagaaag
    12001 tacgtccttt ttttgttcgg atattgttga aggaaagctt taacgctgtc caaaaactca
    12061 tctttgttct gcgtaagtta aattgatcat cttttggtcg ttcgatgtga ggatgggaat
    12121 ctgttaagat cttaccatca gaaaattcaa tgattcgatc tgcgtattga tgggccagtt
    12181 caggattatg tgtgaccatg atcactaatt tttctttcga taactcttgg atcaattcca
    12241 tgatctggat acttgtttct gtatctaacg ctccggttgg ttcatcgcac aaaaggatat
    12301 ctgggtcatt ggctagtgct cgagcgatgg ccactcgctg catttgtcca ccagataact
    12361 gatttggctt tttatgcata tgatttgtca agccgactcg gtgcaatgca tcttctgccc
    12421 gttttcgttt ttcgtcttta gaaacaccac ttaatgtcat cccaagttca acgttttcaa
    12481 taatgcctag atgaccaatc aagttgtaac tttgaaaaat gaaaccgata gagttgtttc
    12541 ggtaggcatc ccagtcactg tccttaaagt ctttagtaga ctttccgttg attaccatat
    12601 caccggaatc ataattatct agtccaccaa tcacattcag catagtcgtt ttaccagaac
    12661 cactaggacc taaaatcgct acgaattctt tttgtctgaa agcgacagac acaccatcca
    12721 atgctttggt ggtggtttcg ccgactttat aatatttttt tatatctttt aattgaagca
    12781 taatcaccct tctcccgtat tgaaatattc ggtatactaa taagtatatc agaacaatga
    12841 tcgtagtata cgaaataaat gttaactgaa gatgaactac aagaaaaaat ctagaaattt
    12901 ttcaaaaaac agagggagat caatgaatcg tattttagta gtagaagatg atccgcaatt
    12961 acagttgctt taccgttctg tgttggaacg agtgggcttt tcagtcatca ccgcattgaa
    13021 tggagaggat gcgctgaaaa aactagaagc gacacgtata gaattaatca ttacagatat
    13081 catgatgccg aaaatggatg gctatgactt gctagactca ttacgtagca ccagatttga
    13141 cacacctgtt ctaatgatta cggcaaaggc cgattttgag gataagaaac gcgggttcaa
    13201 attaggagca gatgattata tgacaaaacc catcgatgtc aatgagatga tcctacgtgt
    13261 ggaagcattg cttcgacgtt caaagatcaa ttacagcagt agcttgataa tcggacaaac
    13321 aaaattagag ctagatgctt atacagtgtt acaagatcag cattcgttga ttctgcctca
    13381 aaaagaattt ctgcttctgt ataaattact gtcttatccc aacaagatct tcactcgcca
    13441 acagttgatg gatgatattt ggggattaga tacgaataca gaagaacgaa cgatcgatgt
    13501 acatatcaaa cgtttaagga cccgttttga aaagacggaa gattttcaaa tcatcacagt
    13561 acggggacta ggctataagg cggttattta aagatgaaaa aaaagctatt ttcacaatta
    13621 tggatctatt ttgctagtat tgtcttcatt tcgatcttag tgacactgct gtgttttctc
    13681 ggttttattt tcttattgtc tcgacattct atctcgcctg ctgaatcggc tggaaaacca
    13741 acgttatacc cgttattcgt ctttgcgggg ttcagtatgg tcgtgggtac aggaatatcg
    13801 atttttgttg ggcggcgtat cctgcatcca atcagtgcgc tagggacaaa tatgagtctg
    13861 gtagcaacgg gagatttttc gattcgaatg gatgaacagc agaaagtagc agaagtacag
    13921 caactgtata aagatttcaa cgttatggtc caagaactca atagtatcga gacactgagg
    13981 aatgactttg tgtccagtgt atcccatgaa ttcaaaacac cactagcaac aatccaagga
    14041 tatgttcaat tgttacaagc cccaaatctc tctgacgaag aacgccaaat atttttatat
    14101 cggattatcg aaagtatcac gcagctttca caattaacag agaacatctt aaaattgaac
    14161 aaactggaaa accagcgtat ccaactggaa aagaaagagt atcgattaga tgaacaaatc
    14221 agagaagtaa tcgttttttt tcagcctaaa tgggagaaag aacagctaga attggatatc
    14281 gaacttgctg cagtaaatta cacaggcaat gaagagtttt tgtaccaagt gtggctgaat
    14341 atcatggata acgccataaa gtacaatcaa atcaatggac agattcatat caaactattt
    14401 gagacagcca cagagattgt actggaagtg accgattcag gtgtaggaat gaatgaagag
    14461 accagagatc gaatgtttga aaaattttat caaggagata caagtcgtca aatttctggg
    14521 aatgggctag gactttcact ggttaaaaaa attctagaac tgcatgatgg acgtattgat
    14581 tatagcagta tcgaaggagt aggaacgaca gcaatgatac ggttaagtaa acaataggaa
    14641 aaagaataaa acccctaaaa attctattga aggtttttag gggtttttag tcgttttttt
    14701 gtgattaggg aaatgttctt acggttcttt tggcataaaa atcgctgctt tcttattgaa
    14761 ctacgagaaa tgttagaggt gtcattgaaa caaggacatg atgcaagaaa gagagcgaaa
    14821 gtcatgcgtc gctctctttt tttgggtgga tagatggaca gaaaatgtag ggcttagttt
    14881 tcttcttgat ttaaatactc ccaaaatgta tctgaagcca aagaatgctc accgacttta
    14941 cgagtaaatg caagataact ctctgttgtt ggagaaatag ggagaatcac agtattttct
    15001 tttaatagat tcttgactgt tttttccata acaatagcaa tccccatgtc atttgcaatc
    15061 atattgatga tcaagtcgat tcttgctcct ttataagaga tttttggttc aaatccagct
    15121 tcgcgacaca gatctagtac aggttgatac aagttcgtag tggggccaag gagcaaaaag
    15181 ttctcctttt tcagttcgct tagatccaat ttctttttac tagctaaagg atgattttta
    15241 ggtagaactg ctacaaaatc gtctgtttca gtaggcaacc actcaatcga acttggtggg
    15301 gcctggaaac ttctagcaaa gtggatcgtg tcactatgtt cattggcgtc attgtaagtt
    15361 aattcgacac tttccacttc tttcaactgg actgtaaatt caggatggcg tttcaaaaac
    15421 tcggtgattt tggaaaaacc tttataattg atcattgtcg gtatcgttaa gatatttaat
    15481 gaaagattgt tgtctttcgt ataggtttca agcacttctt gcatagcatg gtattgttct
    15541 acgacatttt ttacatatgg gagcaataag cttccagctt cggtcaattc gatttttcta
    15601 tgggcgcgtt cgaataattt gacgtctaat tctgtttcta acgaaagaat ctgtttagaa
    15661 atatttcctt gggtggtgaa ttgtcgatca gcagttttgg tgaaattcat tgtttcggct
    15721 aaatcaagaa aaacttcgaa tttatggata tccatcatat tcctcctact tattccaata
    15781 aagaatactt taacacaaaa aatgaattgg tgtaatcttc tctgtctgtt acaatctttc
    15841 ttgcagcgaa agcaataatt acagaaattt ttctgatttt tttcgcttcg tagaaaaaga
    15901 gaggagtttt ttgatgccaa ctggagtttt gatcaatgta ggttccgttt tgtttggcgg
    15961 actaattggc ggattagtag gaaataaatt aagtgaacat tttaaagcac agctgacgat
    16021 ggtttttggt gtgtgttcga tggggatggg aatctattcc attgcgccga tgaaaaatat
    16081 gccggctgtt atttttgctt tagtgatagg aacagcgatt ggtttgattg ttcatttagg
    16141 taatgggata aataaggggg ctgcgctgat gcaagtacct atttctaaaa tcttccctag
    16201 tgaaaagctt ggtatgaccc acgatgaatt tatcagcaca ttagtgacgg tcattgttct
    16261 attttgtgca agtggtacgg ggatctatgg aagtttagat tctgggatga caggagacag
    16321 tacgattttg atttctaaat ctgtccttga ttttttcaca gcagctattt ttgcgtgtaa
    16381 tttagggtat gtcgtttctg tgatagcaat tccccaattc attatttttt atattttgtt
    16441 cttattggca aaatttattt atccattgac tacaccagac atgatcttag attttaaagc
    16501 ttgcggcgga ttcttgatgg tcgctaccgg ttttagaatg atcaacgtga aaatgttccc
    16561 agtagctgac atgatacctg caatgatcgt tatcatgcca ctgagctgga tgtggacaaa
    16621 ttggattatg ccactgttat aataaaaatc aaagagaagc cattttttgc tgactaaaaa
    16681 ggatcaaatc ttttagtcag tttttttgta tttgacaaac tgattttgaa actatattat
    16741 atgttgtata atatagattg aaataaaata ttacacttga gaatagaagg tgacgtaatg
    16801 cacatccaga ttccaactgt tcttcttgat ggtactgtgc tagcagcatt aagcaaggaa
    16861 gatatgtatg ggtatgcctt aacaaaaatc gtacaagaaa gtctctctgt cagtgaatct
    16921 acactatatc cggttttgag aagattgaaa aaagaaggat atcttgagac ctatgatgaa
    16981 ccttttgaag gcagaaaccg tcgatattac tcgttgacac caatgggaag tcgtcattta
    17041 gaagaaatta ttgatgaatg gattgttttt cgagattctt tagaccggtt attggaggag
    17101 agcacaaatg aatgaatatt tgaatgaagt tgtggatcat cttcaagaaa ttcctgaaga
    17161 cgatcgtttt gatttgatcc agtattatga ggaatatttt ttagattctg gaaaaacatt
    17221 agatcagatc attgatgaat atggtacacc aaagcagttt gccttaaaat taaaaatcag
    17281 ttacttttca gaagaagaac aagcagaaaa cgagttagtg aaaaatccaa aaagacaaat
    17341 tcgcctgatt tggttaatca ttatcggttt atttgcttct cctgtcctca ttccactagc
    17401 actggggttt atattggtta ttgggggact attggttgct ttactagcgc tcatttttag
    17461 tatctatgtt ttatgtatca gtatcttagg tgttggccta ttcatgatca tctcaggaat
    17521 cagtgtgttt ggacaatcga ttctgagtgg attattcttt atcggtgtcg gtctgttcgc
    17581 cacaggaagc gcagtattta tttcgccact gttgctgaaa ggaacaaaag ggctttttta
    17641 tgtcatgatg aactttgtta aatggatggg acgacgtttt attacaaaaa gagatctgca
    17701 ttcagcgggc gtttgaagga ggacacataa attgagattg aaacattttt tgattaccgg
    17761 tattggtctg atgcttactg gtggcctcct agcaggtgca acttatacat taggcgctca
    17821 aaagagtctg acttgggata atggtcctcg gttgatcgat atggttactg agacaaaaaa
    17881 aatcaaggat gcaaacaaaa tcgtcatcca tgcagaaaat caaacagttt cggtcatacg
    17941 cggattggat tttgagataa aaacgactta tgataagaca gaaaagccag aaataaagga
    18001 gaaaaatggc gtgttgactg tgaatgcagt caaaaaatca acaggtgcaa tcattagttt
    18061 agaagacaca agtgcacaaa tcactattgt tgtccctgca gatcatgaat tatctgaatt
    18121 gacaatcgaa gggaataata gtgcaattga tatcaacgaa gtatcaatca aaaaagtgaa
    18181 tactaagaca catgatggct gggtatcgtt caatgaattt acaggaggaa caataactgc
    18241 gaaagatgaa ttaggctcac ttcgattaga tgaggccaat gtggataact tgatcgtcac
    18301 aggtgaaaac agcaacttcc atctagaaga cagtttgatc agcaaagaag gaaaagtcat
    18361 cctaaacggt ggcaatttat ctttgcatga gatggaaaac agtggctata ctatccaaac
    18421 gtctggtgaa tcttatattg aaaaagacta ccagccaata acaaatactt tgttagaaca
    18481 aggacagaaa aagctaaaga tcactgctaa agacacatcg atcaatatta cgacggatca
    18541 agaggacaat gaaacagatt gatcctgtac tcaactggat attttaagaa aaaacagcaa
    18601 agatccttcc aatctttgct gtttttcatc gcttaacgca cataactata gtaaaataag
    18661 aaagaaaata tctaaaaaga aaacagggtg aaagagtata tgaaggtaga agaaggtcaa
    18721 agagaaaagt taaaaacaga aatggaaaga ttacatactt atatcactca actaagccaa
    18781 accttctatg atccagacaa agaaaaagtg atggttaatt acccgaacaa ttcagaaggt
    18841 cgccaattag agcaagtgta ccatgaagtt ttcaaacatt tattgacggt gaagaaagag
    18901 ctggattatt attctttacc gattatcgat acaggaatat tgaagtatga tgggaagaag
    18961 gaacgattta tctttaaatc tgttcgggaa gatttgcctc tttcagcagg gatggatcta
    19021 gaggtcttgg tagaagacta ttttacagaa gaaaagcact gggttcgaac aaaattggat
    19081 tatttaccgc aggcagccgg agggacacag gcaagcggtt ggtacatcac tgaagacaaa
    19141 gagttagaac tagaaggtgt aatggcgcgt attcgaaaaa aaaattaaaa aagtaacatt
    19201 agatatttat tgaaggataa aggagcgcag gaagtagcat gagagaaaaa agagcatttt
    19261 atatcaatgg ttatgcaggg attgtcggat tggtcgtatt agctttagtt ggattgtttt
    19321 tcttctattt aggtatgtgg caagcgaaag tttgggcact ttttttaagt atttttcttt
    19381 ggttgatcac gttattgttg ttaagttcgg caactgtagt cagtcctaat caagcaaaag
    19441 taattttgtt ttttggacaa tatttaggta cgattcgaga aaatggcttt ttccttacga
    19501 tacctttggc acaaaaaatg actgtttcgt tgaaagttcg gaatttcaat agttctgttt
    19561 taaaagtcaa tgatttggat ggaaacccca tcgaaatctc agcagtagta gtctttaaag
    19621 tcgttgatac agcaaaggca ttattcgatg tagcgtatta tcaagatttt gtagaaatcc
    19681 aaagtgagac agccattcgg catatcgcta gccaataccc gtatgataca ttcaatgaga
    19741 atgatttgac gcttcgagga aacacgacgg cagtgtctga cgagttgcaa aaagagctgc
    19801 aagagcgttt agcagtagca ggtgtcgaag taatcgaaac acgcttgaac catttagcct
    19861 atgcaacaga aatcgctagt gccatgctcc aacgtcaaca agcaaaagcc attttgtctg
    19921 ctcgacaaac cattgttgaa ggagctgtca caatcactca aatggcctta gagcaaatcg
    19981 aagatggaca agaaatcaat ttcaccgatg atcggaaagt ccaattgatc aataatttac
    20041 ttgtctcaat cattacagac aaaggaacgc aacctgttat caatacaggc gacatgaaag
    20101 accgtaagtg acatactttt tgtaagatag gaagaaggag tttaggacgg attattgtct
    20161 tgaactcttt ttttagttgt tttaaagaaa atgacaaaat tgaaagaaga atgatagttg
    20221 attcgaaggc agaattgttc gcttttttct ataatcagta tataaagtaa gagagaaggg
    20281 gaaaaagtga aaaaacttct gatcggtcta cttgttttca tcgttgtggt acttgcaggg
    20341 gtgaaaatcg cagattacgt agttatgggt ggagaagaat actatgtaca aattacgaca
    20401 gatggtacaa aaacagtttc aaaatcagac tctggacaag aaacagtcaa ctatagatat
    20461 atcctgcctg gctatacgaa agagggtaag gaaaaagaac tggacttcaa gggacaattg
    20521 actcgaccgc ttcgtaaagg agcatattta aaaatcacct ggaacaaaaa taaaggtgtc
    20581 acttcgtatg aagaagtcga aaaaaaagac gtgccgaaaa cagcactgga aaaactaatt
    20641 aaagggtagg gaaagaaatg aacaaaatag tcgaagtaca gaatgtaaca aaagtatacg
    20701 gaaaaaacaa tgagaaaaag acgcaagcat taagcggcat cagcttcgat gtagaaaaag
    20761 gcgaatttat tgggatcatg ggcgcttctg gttctggtaa gtcgacactt ttgaatattt
    20821 tatccacatt agataaacca acagatgggc atatccgaat caatcaaaaa gatgtgacca
    20881 cattaaaagg aaatcaacta gcagattttc gtgcgaatga gattggcttt attttccaag
    20941 atttcaattt attagaaagc ttgacagcac aagaaaatat cgctgttcct ctttctttac
    21001 aaggcgtcag accaaaagaa atcaagcaac gtgtccaaaa aattgcagaa cgtttatcca
    21061 tctctcatat tttggaaagc tatccttctg agatttccgg tggacaaaaa caacgggtcg
    21121 ccgccgcacg tgcattgatc acacaaccaa cgattctatt aggggatgag cctacaggtg
    21181 cgctggattc caaaagtgcc cgggatcttt tagatacgat ggacgaattg aatacaaaag
    21241 atcatgtatc gattcttctt gtgacgcatg atccgttttc tgcaagttat tgccaacgta
    21301 ttttattcat caaggatggc ggtatccatc aagaagtgaa acgtggagat caaagccgtg
    21361 attccttcta tcgagaaata ctatctattc ttggaaactt ggaacagtag ggggagattg
    21421 actatgcttt ggaaattatc acttactggg atcaagagcc gtttaagaga ctacttggtg
    21481 ttattctcag gattagttat ggcatctgct attttttaca tgtttgaatc aatggcaagc
    21541 aacgaagctt ttttgaaaag taatacgatc gttgcggcta cagttatcat tttccgattc
    21601 ggttctgttt tactagggat catcacattc gtttatattt tatatgcaaa ttcctttttg
    21661 atgacgatgc ggcaaaaaga ctatgcaatg tttatgatgc tgggagcaaa aggcagaaaa
    21721 atcgcacaga tgattttttc tgaaacattc ttagtaggga tcgcagctac tttagtgggt
    21781 tcagctgtcg gtgtaggatt gacaagtatc gtccaccgat tattagtgga tcagttagat
    21841 ctcaatattt cgcattttac accttttagt attcacgggt tgttgatcac tgttttgttt
    21901 tttgctgttt tgtttttatt agctgctgtt gtcaatgctt tttcgattgt caaaaaaccg
    21961 attttagcat tgattcgtgc ggatcagaca ccaacaagaa tcaagcagca taaattcttg
    22021 tttttccttg aagtagtact gggaattgct tttctaggct gcggttacta tatgatgaaa
    22081 cacgtgggga cctatcaagt ctttggtata ggggtagcct tagttacgat cgtattagga
    22141 acttatttca ttttccattc tgtgatcatt ttctttttat cattactaaa gaaatcagaa
    22201 cagatttcat tgaaaaaatt gaacaatttc acattgtcac agttaaactt tcgtattcgt
    22261 gaatatacac aaatgctctc aatggtcgct atgctgtttg ccttggcact gggcgctttg
    22321 acggtaggat taggttttaa aaatgaaatt gcagacttga cagaaaacaa ttctggatat
    22381 gatctagtga tgaataatcc tcgggcagaa gatcagcaaa aaatcaaaga actttcacca
    22441 acactttctg cttcttatac tcaaaaagaa gatgcgggaa cgatttacta taatgcaagt
    22501 gaatttgcgg ctcaaccatt gatcatgata aaacatgaag atggaacacc gccgaagatc
    22561 acgaaagaaa aagttcctgc taaaaaaatg aatgaactga ctgtacaaga ggaacttcga
    22621 agctatgaat tgccggaaca gcaagaaaaa gaaatcaagg tagtcagtca atcagaattt
    22681 gatcaactga acttgccaca atccacttta cagctagttc aggtaaaaga cttccaagca
    22741 aacctgaaac caatcgaaac acttgttgct caaaataaaa tgaacaatcc atctttgcaa
    22801 aaagtagagt acagcaacca gaaatatgaa atgtatgaag tgtataatgg tctattctct
    22861 ggttttgaat ttatgggatt cttcctaggt ttagctttct tgaccatgct tgctagctgt
    22921 ttgatgttca agatcttgtc aggaagcaag agtgacattg ctcgttatga aatgcttgaa
    22981 aagataggaa cacgacgcgg gttgcttaaa caatcgatcc gtagagaagt aggcgtgtta
    23041 ttcttagcac caggaatctt aggtgtagtc catgtcttat tcggattaca aatgtttggt
    23101 ctattcatgc aaaatccata caaagatatt tgggttccgt ttacgatatt cttcgtattg
    23161 tatttcatct actatgtact gacgacatgg ttatatactg gaatcgtatt gaaaaagaga
    23221 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agctcctcat
    23281 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
    23341 aaaaagaggt tgtgacaata tttttgtcac aacctttttt tcacggtctt ttattagaat
    23401 atagtaaatt ttttctcgtt actcacggaa taagctgtta agaaaacggt ttccatgtat
    23461 actggaatca gtgcaaatga aagaaaagag ggtgcaagga acatggaaat caatgaacaa
    23521 aattttggcg aaaactatca cttgattacg atcacgaatc aaaatgggac atctttgtct
    23581 gtcagtgatc ttggggcacg ggttgttcat tttcaaacaa taatcgataa tgaaaaacga
    23641 gagttgatct taggttttga tgaagcaaaa gaatatctag aaaaagaccc ttatatcggt
    23701 gcttcaatcg gacgtatggc tggacggatt acgaatggca cattcaaatt agatgatgaa
    23761 acgttcgaag taaaaacaga cccggacact ggtcattgtt tacatggtgg tgcacctagc
    23821 tttgaagcca agcagtggca ttatacagtc atcaatggtg aaaacgaagc atcggtcatt
    23881 ttctatcaga ctagcccaga tatggaaaac ggatttcctg gaactttaga tgtagaagta
    23941 cggtatacgc tgacgaacga tgatacatgg cgagtgacgg tccaagggat cagtgaccgc
    24001 aaaacagtat tcaatcctac aaatcatgtc tatttcaatt taaccggtga tccagcacag
    24061 tcaatcgacg aacatatact ttggttgaat agccgattat ttgctacgct caataaagat
    24121 acaacgccta caggagaagt agtttcagta agtggcacag catttgactt ccaacgtcct
    24181 aaaaaattag gtgaggtctt tgccagtgat tttcgccaat caatgatagt gggcggcatc
    24241 gatcatccat ttttgttgaa aaaacaatct ggtttactaa atgctgcgga attgacaagc
    24301 ccagatgaaa aagtatcgat ccaagtaaaa acagatgctt caggaatcgt gatttttaca
    24361 gcaaattttg gagaagatgg accggaaatg cgcgggaata aattagtaaa tcatggcggt
    24421 atcacatttg agactcaaga actgccagga gcggagcgac ttgaaagttt tggagatgtc
    24481 actctggaac ctaaccaagt acgcgagttt gtaacggaat acaaaataaa tataaagaaa
    24541 tagaataaat taaaaaggga gtgtgacagt gtcacatccc ctttttaatt tattctattt
    24601 ctttaaaggc agatcaagcc agaatgattt gtctaaactt aacaaggtta gtacaggaac
    24661 agttgcagct ttaactgtac gagtagttgt ttctggtatc tgtcttgccg taaaattgtt
    24721 ccacaaatcc cagattgaag cgacacggaa gatggaagaa gcgttgttga cgcgttttat
    24781 cgttgaaaat tctaatcctg tgattggcac gccatctgtt gtccatttga ttggcacatc
    24841 gaatcgctta gacatattga ggatttcagc gtttgcttct tcagttgttg ctgcttcttt
    24901 tttcggttca tcatattttg tcaggtcgta cgcttcgatg atcgcgttca atttttgtga
    24961 atattggcga tctgtagcat aacgtccttc caaatatttg gttgcttctt tgtagtcttt
    25021 tgtttccgac ttccaagttg gtttgtagaa atcaggattg ccagtcagtc catttttgat
    25081 caagtcggca tagtcttcca aactttcctt gtaagaagga tatttacgga attgtgcgcg
    25141 gatttggtag ctttgtccgg aactatcttg ttcaagtgta ttgaaggtca cgctgcttcc
    25201 tttatatgat cctttgatac caaaaaggtt atgatttggt tcgcttgata ataagctgtt
    25261 tccagatcca ctttcaagga tagcttgagc catcatgact gatgcgtaga ttccttcttt
    25321 ttgcccgatt tcctgtgcat catctgcaat ctttttcaag aactgatcag ttgtttggtt
    25381 ttgactgaaa tggaacgagc catctaatcc atctaaaata gatgagccgc ctgtcagcga
    25441 acctgaagca gcaggtaaac ctgctaagtt tactcggaca gcaaaatcag gcgtgaaatc
    25501 tgaaatagaa gttactcgaa ctttatctcc tggttgaggc gcatggataa attgtccgtt
    25561 cccaatatag atacctacat ggtgagtaac accggaagaa ggtccccaga agacgaggtc
    25621 gccaggttgg gcctgactta ctggaatctg cgtacctgca tattgctgtt cgcctgtcca
    25681 gccaccgata taatgaccgg ttgctttcat atatacgtaa taagtcaaac cagaacagtc
    25741 aaaagtattt ggaccttttg ctccccatac atatggtttt ccaagatgct tgaatgcttc
    25801 tcgaacgatt gcttgttgga cattcgttgt atttgtaggc aatacagcat caattggtga
    25861 ttttacaaca aaatcagaag aagcagaaga tggattctct ataccgcttt gatttgctgt
    25921 atttggacga gatgaagcat tgttcgttcc gccattcgat gaaccattgt ttggatggtt
    25981 tggttctctc tgagtatttc cattgttatt cgatccagat gggtttgttt gattcgaaga
    26041 attcccgctg ttcgaattat tgttattatt gtttgaatta tttgaaccat tattattgtt
    26101 gttattaccc acatctggtt tgtttggcgt ttccggatct ggtgtactag ggacttcttc
    26161 actaggatca gttggtttct ctgttgaagt atccggtcca gctttaaatt ctgcagtaat
    26221 agataaattg ttcgtaccta ctgtaaaggt ctttgcatca taatttactt ctttggcacc
    26281 attaacagcg actgaagcaa tttgttcacc ttcagctggc gtaatagtgt aagttacctt
    26341 cgtttccgct aataaacctt ctaccgttcc actttccgta atatccaaag gttgctgttc
    26401 aggggaatcc gctgtcgaag cagtcagttg gatcttacca atcaaagaag gagcaattgt
    26461 aagggaatat gtctcggctg gcttttcatt ttctgatgga tcagccggat caatttcagt
    26521 tggctcggtc gatcctgttg ttgagttagt tgttccagtt gggtcttctg taaaagaatc
    26581 cgttgtttca ctagtagctg agctggatgt agtcgtatct gttgtgactg tttgatcagc
    26641 tatttgatta tctacggcaa atacggtatt ggtcgacaaa acagacggag caatcagcca
    26701 agtagcagct agcaaacgta tcgttttttt atttttcata ataaactagt ccttttcctt
    26761 tcattgattt tctgtcttat tcagcattca ttagacattt tttggaacag ataatcgtgt
    26821 cgaaaagctt tttcttttca taaatctgtc tgtgtattct aaataaaaca atttgtaaat
    26881 aagcaacgag cattcattcg tttttctttg acgttcattt tattgcatag accacctttg
    26941 tttggggata aacaagtggg gataaaaatg gttatgcata tgtaaattat accaaagttt
    27001 ggaaagtatc acaaaaattt ataaaaataa tgatcctaaa aagaaaaaga ctgaaatatg
    27061 tgataaacat ctcatacttc agtctttttc attaactatt cataagacat taggtcttca
    27121 tcaaacccgc taacgagagt agtgatcatt gctacgacta gactaacagc cagtcctgct
    27181 aagtaccaga aaaaagtacc ggaaatgaat gcaggcaatg tggtcagact accaaataca
    27241 aaagcgatcg ctttcacttg ggcaaatccc attacagctc caccagcagc tgcaccaatg
    27301 caagcagcaa caaatggttt tttcaatggg atcgcaattc cgtaaacagt tggttcggtc
    27361 actcccgcaa gtaaaatcga agcaaaagca gaaccggaga tcgaacgggt ttttgaacgt
    27421 ttacttcgca gaaatacacc taaagctgca ccagccattc caaaattggc tgctcctgtc
    27481 aacggaacga ttcggtcgaa cccataagca gagatattac tgatcatgac agggtttaca
    27541 gcccattgga taccaaatat taccaaaata ttccaaccac cagcgatgat tgcgccactg
    27601 atcggtccgt taatatccat catccaagta attgcggcag ctaaaccttc gccaaggtaa
    27661 acacccgcag gcccgaatac cattaacgtc aaaggaaccg tgataactaa tgaaagtaaa
    27721 ggtacaaata ctaattgcat attttcgcga atatattttt tgaggaattt ttcaacaaaa
    27781 gaataaaaga aaatacctaa aatcgccggc atgacagtag acgtatactg catcagtact
    27841 acaggaatcc ccagaaaatc agtagctgct ttgccactgt ctgaaagtaa agaggtcaga
    27901 ttcggttcaa tcagtgctgc agcaatgaca gccgcaatat atccattcac tttaaacgtt
    27961 ttggcagcag aaaaagcaag aaagattggc agaaaataaa agacggcatt cgacgcagca
    28021 gacaaaatat gataagttcc gcttgtttca ctaagccagc caatcgtagt cgctaaaaca
    28081 accagacctt ttaatatacc agaaccggca aacagcggaa tgatcggcgt aaagatttcc
    28141 gatatcaaac caaatagttt ttcgatcgga tttagctttt ctttcggagc tgaatgatcg
    28201 atttgacgcg tcaattcctt gtaagcagga gccaatccct gatccatgat tatttctgca
    28261 tatctatcga gtaaaatgaa atacttaatg gaagggattt gctgttttaa tttttctgca
    28321 ttggctactg gctgtttttt caatttgata cgaagccgag tcgcacatgg aaggacagaa
    28381 tcaatattgt cttttcctcc caactgttca atcagcatat gaatctgctc ggacagtacc
    28441 atctgttcag gattcattgt cgtttccatt agaagaccac tccactcact aatataatat
    28501 tggcgtaagg tgtcatttcg cctgtacgaa taaatgcatg acagttattc aactcttttt
    28561 tcatttcttc atgtgggata aagctaatcg gtgtatctgg taacaataat ttgatttttt
    28621 ccaatatttc cgggttcatt gtctttattt cttccgccaa ttcaatcgat tgtacctcaa
    28681 gttcttctaa aacgttttct aaaacagata aaaaacttgg aagaccgttt gagacagcca
    28741 aatctatttt ttcagtacca aaggggacag gcatgcctgc atccccgata cttaatttat
    28801 caaagtgccc catttgtgcg attactcgtg aaatatctga attgattact tttccttttt
    28861 tcatgtttgt ttcttcctct cattgttaat tttagactgc tgattgttct aaagcttcgc
    28921 ggtaagggat agaaggttgc gcaccataag actgcacagc aacagaagag gctagatttc
    28981 cataaaggat cgcttctttc aagttactaa ggtctggctg aagtgtgctg cttaatgcgc
    29041 caataaatgt atcacctgcg gctgttgtgt caacggcatc cactttaaaa gcagggacga
    29101 taccgtgttc tttttctgta tgatagaaag cgccggcact gcctagtgta atgattactg
    29161 ttccaatacc taattcatgg agtttttcgg ctgctgcgac caaactgtta tgatctgtca
    29221 ctctgattcc tgtaatgatt tctgtttctg tttcatttgg tataatgata tctgtctttt
    29281 caagtagttc agtaggaatc gtttcacgag ctggtgcagg attcaggatc gttgtttttc
    29341 ctgcgtcttt tgcaatagaa aaagctagga tcgtcgcatc taagctactt tcaaactgag
    29401 caataataaa gtcactgttt gtaatcagtt gcttgttttt taggacttct tgttcatgga
    29461 aagcattatt tgcccctgca tggatcaaga tactattttc tcctgctgcg tctaccataa
    29521 tcattgcttg tccggttgtg gcgccttgga tttcagctat cccagatgta tcgatgtttt
    29581 cttttgtcag tagatctaat aactgttgtc cttcgctgtc agccccaacg cctccaataa
    29641 aagatgtccg tgcgcctgaa cgtttggctg ccactgcttg gttggcacct ttacctccgc
    29701 cagcgtgaaa aatctcatgc gcatgcatcg tttcgcctgg cttgggcatg ttggttaaac
    29761 gaagggtagt gtccatatta atacttccaa ttactgtaac cgtgttcatt ttacaaacct
    29821 ccaactatct catttatttt gatgggaaaa ttatttttta aaacgtttta gtaaaacgtt
    29881 ttaatagttc gataaaaata tagcacacct tgaaagctat ttcaagggtg cagttgaaaa
    29941 tcttttttct aatcgtacag gtaatctttt ttcttcccat tctttttctg ggaattggat
    30001 ccgatctaac aataacttgg cagatgtttg tcccaattcg aagattggtt gtgcgatcgt
    30061 tgtcagtttc ggcttgatat actcgcacat atctatatta tcatagccaa taatgctgta
    30121 gtcttcaggg atgctttttc cagcttcctc taatccgcga tataatccaa aagctaattc
    30181 atcatttaaa gcaaacacac cagttgcagc agaatcaaga agttctgctg tgatctggta
    30241 gccaccttgt tttgagaact gtgtaggtaa gaggatcagt tggtcgtgag ggatttggta
    30301 aacatcaaga gcagatttaa acccctcaat ccgagcgtgg acattttccg gtggattttc
    30361 aggatagaca agggcgatcg tttgatgtcc tagactaagc aagtgcatgc ccgctaggta
    30421 accaccgcga aaatcgtctg ttcgtacggc atcactaaat ccttcagatt ttttctgatc
    30481 caaaacaatg aagggccggc cttgtttttt caaattttcg ttgattgcat ctgtagagac
    30541 agcagaagtc gcgataatga atccatccac acctcgacgt gtgagctcgg ctagatattc
    30601 gatttctttt tgatgatcag aatcagcatt acataatatc gtgacgaaat tctgcttata
    30661 caagatatcc tcaattcctc gcatcagtgt actgaagaat ggattcgtga tatcaggaac
    30721 gagtacacca attgttttcg tttccttggt aatcatttgt ctagcaaaat agtcaggctg
    30781 atagcctaat tcttctttgg cagctaaaac tttctgaaca gttttcggac tgaatttctc
    30841 cgtatttcca tttaatatca aggagacagt tgtgattgaa acgccggaaa gtttagcgac
    30901 ttctttgatt gtgatctttt ttttcaccat gttcatactc ctatctaaat cctgtggtta
    30961 ttttcccatg aaatcaaaaa gaagtaaatg aaaatcacgt gtgccagtac ttatttgctt
    31021 tgttggatag cttcgattat ttgtttcgtt gagcgtactc gtgcagtttt cggaaatatc
    31081 ttttttatga acaattgatg ggattctttg tcacgatcac tgcaagcatc ttcagcaagc
    31141 acgatatggt acccgtgttg aaaggcatct aatgcagtag catatacacc gtttgttgtc
    31201 gctacaccgc ttaaaatgat agtttcgatt ccacgccggc gaagttgaag atcaagagaa
    31261 gtaccaaaaa aagcacttgg attgtatttt gtgatagtca acatattatc tgtatctttc
    31321 aaagaatcct tcaaaagtaa atcagtgaat tctggaggta cagaagcagt cattccaccc
    31381 atatcagttt gttgactcaa ataagggaaa gtggagatgt ctacgttcac taaaactttc
    31441 aaggcagcgg tacgtttcaa tgttcttact aacaaatcat tgtttgtcag aatagatgct
    31501 gcagtgtgtg gagagagtgt accgttattt acgatctctt tttgcatgtc gatcgcgaca
    31561 aaagcagttt ttgataaatc aaaaatcata ttttccttta aatcattcat gatgttcctt
    31621 ctttcaaata acaatgtttt ttatgtaagt aaatcataaa tcgtttattt gtcaactaaa
    31681 ttaaaggatt cctttcttta gtctagagaa attcagtggt aaaataaata agtgtgagaa
    31741 aacgcattcg atgcatagat atagcgagaa atatcgtgaa aatggaggaa tcagagtgaa
    31801 gtatgtcgaa gtaaaaaatg aatacaaacg atacaaaatg ggagaaacga ccattacagc
    31861 aaatgacgat atttcttttt caattgaaaa aggcgaatta gtgattatac ttggtccaag
    31921 tggagcagga aagtcaacga ttcttaatat tttaggtggg atggatacac ctgatgaagg
    31981 tcagatcatt attgatgaca cagatattgc gcaattttca gataaacagc ttactgctta
    32041 ccgacgaaca gatgtagggt tcgttttcca attctacaat ctcgttccta atctaacagc
    32101 aaaagaaaat gtggaattgg caacggaagt ttctcctgat gcgcttgatc ctgtagaggt
    32161 attgaggcaa gtgggcctag ctcatcgatt gaataacttt ccgtcccaat tatcaggagg
    32221 cgaacagcag agggtttcaa ttgctcgtgc actagctaaa aatcccaaac ttcttttatg
    32281 tgatgaaccg acaggcgcgc tggattttga aacgggcaag caagtattga aattattgca
    32341 aaacgctagc agacaacacg gcaataccgt tttgatcatt acacataatt ctgctttagc
    32401 gcccatcgct gatagagtca ttcacattaa tgatgctaaa gtaaggtctg ttgaattaaa
    32461 tgatcatcct tcatccattg atgagattgt atggtagggg gattttatga aaacaagaac
    32521 atatcttaaa gcaggttttc gcgaaattcg gcaatctaaa ggacgattca ttgcaatcat
    32581 ccttattgtc atgctaggaa cacttttatt tgtaggtgtc aaaactgctg gacctgtcat
    32641 gcaaaaaacg atggatcact atgttggtac agcaggactt tctgatttgc aaatcgtttc
    32701 tactggtggc ttgacacaaa aagatattgc tcaagcagaa aagatcccag atgcaaaagt
    32761 agaaacagga aaacagatct attattccaa tactggcaaa aacgaagtga ttcaaatatt
    32821 ttcttataac aaaaataaca agcagaacaa gctacaatta actgatggaa aactcccgga
    32881 aaaaccaaac cagcttgtat tggatgaaaa agcggaagat gaagggtata aaatcggtga
    32941 tatttatcag attgattcag atgaattgaa agaaaaagag tataccatca cgggctttgt
    33001 ccgttctcca ttgttcatca acaatttgga gagaggatac gcgaacgtcg gaaatggtag
    33061 cgtcgattat tttgtctatt tacctgaatc cgtttttaaa acagatattt attctgtctt
    33121 atatcttgat tttacaaacg taaagaattt gaatacgtac agcaaagctt acaaagataa
    33181 aatggagcaa aatcaagaaa aggcagaaaa gtatttacaa gatcgacccg aagaacgctt
    33241 agaagagctg aaaaaatcgg ccaacgaaga attagaaccg gcacagcaaa aaatagcgga
    33301 tggcaaagca caaacaacgc aagcaagaac acggttagaa gatgctaaaa aacagttagc
    33361 gcaacagtca gcaactatcc agcagcttcc tgaagcgcaa cgaacaatcg ctgttcaaac
    33421 attatctcaa caagaagctc aacttgctga acaagaaagg caattagttg agaaagaacg
    33481 tgaactggcc tctgcagagg aagaattgaa tcgaaatgca gaaaaaattg aaaatttaac
    33541 taaaccatca tacctataca atgaacgttc agaaaatgtc ggttttcaag aatttggtga
    33601 tctgtctgat cgtatcgctg cgattgccaa tgtttttcct gttttctttt tctttattgc
    33661 cgcattgatc acctttacga caatgacacg tatggtagaa gaaaaccgca gggaaatcgg
    33721 gacattaaag gcgttaggtt atacgaaagt tgaaatcgca aaaaaatatg caatctatgc
    33781 aagtctagcc tcagggatag gtattgtttt aggaacgata ttaggaacga atcttttgcc
    33841 aaggatcatt tttgagctgt ctaacgagcg gtatgatatt gggagtgcgc ttatttttta
    33901 tgactggccg ccgattttac aagcagctgc cgcattcttc attgccgcat ttggcgctgc
    33961 aatgatcgtt ttattcaaag atttaagaga acggcctgct gcattgcttc aaccgaaagc
    34021 accgaagcca gggaaacgca tttttcttga atacatcact ccgctgtggt cacgccttag
    34081 ctttaatcaa aaaatcagtt accgaaatct cttccgatat aagtcaagaa tgcttatggc
    34141 gattatcgga attgctggat gtgccgggct gatggttgca ggcgtgggat tgaaagattc
    34201 tctaagttct gtttcagcca aacaatttgg accgatcatt gactatcaag caatcgttac
    34261 attggattca tccacggaag atagtcgtca aaaagcagcg gatatgctgc agaagcaaga
    34321 aaaagtaaat gagatcctag ctattcatac tcaaaccgta gaattgagaa aaaaaggaca
    34381 tgcttcgcaa agtctgacag tgatcgtacc aagtaaaaca gctgatttgg atcgttttat
    34441 ccatttaact gatcttgaag agaacccacg ctccttgcct aaaacaggaa tcgctattac
    34501 gcaaaaagta gcagaactat ttgacttatc tacaggagat catatttcgc tttatgatga
    34561 agatcaaaaa gaatggaaag tcgaagtaaa agaaatcgtg aaaaactatc taggtagctt
    34621 tatttatatg tcccctgaat attatgcttc agttactgat caatcgatga tggataatgc
    34681 atttttgctg cgtacagata agatgacaac aaaagaagaa gaaatgcttt ccgagaaact
    34741 gcttgccaca gacgctgtga caaatacgtc tttcatgtcc aaacaaatcg aaacgcaaga
    34801 agaatcaatg gcgaacttag actcggttgt cttaatcttt gtcgtattat caggactgct
    34861 ggcttttatc gtcttgtata atctaactaa tatcaatatt tccgagcgtg tacgcgaatt
    34921 atctacaata aaagtccttg gattttttga taaagaagtc acgatgtaca ttgttcgaga
    34981 aaatatcatc tttactcttt taggaatcat cggaggattt ggtgtaggat atatattgac
    35041 tgacttcatc ttgcaacaag cgtctatgga gaatgtcatc tttccattag tgatcacatg
    35101 gtgggcttat gtcttatcag caggattaac tatcgtattt acggttattg tgatgattgt
    35161 tacccatttc aaattaaagc atatcgatat gattgatgcg ttgaaatcga atgaataaaa
    35221 acagaggttg tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gcaaaatagt
    35281 ttttcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga acaccatgaa
    35341 tcagaggttg taaaaaagcc gtcctgcatc gagcaaaaag aatactcaaa taaaattagc
    35401 tcctcatgtt ttacatttga gaagactgat tgccgtagat aaaagctttt gaacaccatt
    35461 tatagaagaa acaagaatat tttgctattt taaacaagag gatagacaaa tacatgtctg
    35521 atctcttgtt ttttattggg caacagacct cttagaatgt ataaagataa attttacaaa
    35581 cgctttcggt tcagttataa taataaatga taacttgtat atataagttg tggtaggtaa
    35641 taaatcagtt gaatggaggt aaatatccat gacatttaaa gacaaagtca tttaccaaat
    35701 ctatcccaaa tcttacaaag acagtaatgg tgatggaatt ggagatttaa aaggaatcaa
    35761 agaaaaactc ccgtatctga atgatctagg catcgacatg atttggctaa accctattta
    35821 tccaagccct caaaaagaca acggttatga tatctctgat tacacagcca tcgatcctat
    35881 ctttggtacg atggaggaat tcgaacagct aatagccgaa gcaaagaaat accggataga
    35941 aatcatgctc gatatggtgc tgaatcacgt ttcgacagaa catgaatggt tcaaaaaagc
    36001 actagcaggg gaccagcatt atcaggactt cttcatttta agagaagagc caacggattg
    36061 ggtatcaaaa tttggtggaa gtgcttggtc gccatttgga gatacgggga aatattattt
    36121 gcacttatat gatcggacac aagctgactt gaactggcgg aaccaagcag taagagagga
    36181 actattcaaa gttgttcgtt tttggatgga aaaaggggta aaaggattcc gtttcgatgt
    36241 tatcaatgtt atcggtaaag acgaacaatt gaaaaataat aaagaaaatg atggcaaacc
    36301 tgaatatacg gacaaaccaa tcacccatac gtatctaaaa gaattgaacc aagcaacgtt
    36361 tgggacggat tccactatca tcacagtcgg tgaaatgagc tctacgacga tcgaaaattg
    36421 tatcttgtat acagaaccta gtagagaaga attatcaatg gtctttaatt tccatcattt
    36481 gaaagtcgat tatgaaaatg ggaataaatg gtctactcct ccatttgatt ttgcagaatt
    36541 aaagcgacta tttcatacat ggggagaaaa gatgagtgag ggtaacggct ggaatgctct
    36601 attttttaat aatcatgatc agccacgtgc gctcaatcga tttgtagcta tcgatcagta
    36661 tcgaaaacaa ggagcagaga tgctagcaac catgatccat ttgaaccgtg ggacgcccta
    36721 tatttatatg ggagaagaaa tcggcatgat cgacccagac tattcatcga tttccgacta
    36781 tattgacgta gaaagtttga atgcttataa gttactcctt gaacaaggat tcacagaaaa
    36841 agaagcgttt cgtcggatca aagcaaaatc tcgcgataac tctcgaacac ccatgcaatg
    36901 gtcagacaca gaacaagcag gatttactac agggaaacct tggttaaaag ttggaggcaa
    36961 gctagaagag attaatgtag aaaaagaact atcttctgaa gattctattt tgtcctatta
    37021 caagaaattg attcggctga gaaaaacata tccaatcgtt gcacaaggag attatcatgc
    37081 ttatgaagca aatcatccgg aagtttatgg ctttcttcgc caatttgaag gacagcagct
    37141 gcttgtatta acaaatttct atgataaaga tacaaggatc gccatcccag aaggattcac
    37201 agaagccaat attcttatcg ataattattc cgatactgtt cttcatcaaa gtctggagct
    37261 aaaaccatac caggcaattg ctttgctgaa aaattaaaac aaaaacacaa aacgtcgttc
    37321 ctacgagcta ttctcgtaag atcgacgttt tgtgttttat ttaaaataat cttcttcaga
    37381 aaatgaagag ttttcatact ctcctttatc gccataaaat ttattgtagc gttgtttgaa
    37441 tttacggaaa ataaaagtaa cgagtaattt gactaacgta tagataggaa taccaaaaat
    37501 aacgcccaca attcccatta gatcacccat gacaagtaaa acgattaaga tcgtaatcgg
    37561 atggatctgt aaacgatcac ccattactcg aggaatcacc aagtcaccgt ggagaagttg
    37621 tacggcaaac cagacaatga caaatttgac agccataaaa gtagagtctt ggaatgcaat
    37681 gatcaaacct ggaaagaagg cgataaacgg cccaatatat ggaatgatac ataacaaccc
    37741 agcagaaata gctaaaacac tagcaaattc taatccgatc agtaagaaac aaacccagta
    37801 gactgctcca aggataaatg aagcaatgat ttgccccttt agaaatgaac ccaattgttg
    37861 attagcaatc tttgtcaaat ttttaaaatc tgtacgaaat gctggtggga caatcgctaa
    37921 aactgactga taaaattttt gaggatctct caaaaggaaa aaagcgacaa ttggaccagt
    37981 aaataaagta ataaaaatag ctgtaacagt tgaaaaaatg ttgctgatcc gttgtgcgcc
    38041 actttcccaa taatttccaa taaatccagc tatatcagac gtcacgtcgt caatagaagc
    38101 gaaaaattga ttaaatgaat cggcaaaagg tgttttctcc aaaaattgtc ggagactctc
    38161 ttgaaaatct tgaaacaaat ctggcaaact actgatcaat gtctttgcct gttcttcaag
    38221 aaaaggaata agccatatgc ttccaagccc caataaaaat aaaataatca cataaacgat
    38281 aatgactgac aagttcctcg aaaggtgctt gctcaaacgt ttgatgactg gttccaaaag
    38341 ataatacaag atcagtgcaa acaataatgg gggcaagata gtagcgataa tgattccgac
    38401 aggcttgaat ataaacgaga tctgatggaa aaaataaatg atcagcgcaa gtaagataac
    38461 tattcctaaa acatagtaag aggtttttcc tcctaaaaag cggatcaaac gattcgttgt
    38521 atctgtttct tttttcactc tttttcctcc tttatctata aattcattat agacaaaagc
    38581 gggtataaaa ggaaaatgat agaggttgat tgcaaaaata tcgtcaatca ttcatataaa
    38641 ctttctcatc aattaaaata agaaaacgct tgacagttct tttaaccata tgataaatta
    38701 aatcctgttg ctaaaacgta actattacgg tttgcgaaaa gcgaatcgga acattcaaat
    38761 gaaagaagat gagaagagga gtgtcataaa tgaaacgatt gataggtata ttggccatgc
    38821 tggttttagc aggtatgtta actgcttgcg gggcaagtgg gaaagccgag gacagtaaag
    38881 aaaaattgag cgtgatgacg accttttatc ccatgtatga ttttacaaaa gcaatcgtcg
    38941 gggatgaagg agaagtagag ttattgattc cagcagggac tgattcccac gattatgaac
    39001 cttctgctaa agacatggca aagatccaag atacagatat ctttgtttat aatgatgaaa
    39061 acatggaaac atgggtcccc gcaatccaaa aaacattaca agaaggcaat gtccatacga
    39121 taaaagcgac agaaggtatg cttttacttc cgggaagtga agaaggacat gatcatgacc
    39181 atgaacacgg ggaagaaggc catacacatg agctagaccc acatgtatgg ctagctccaa
    39241 gtttagcaat caagcaggta gcaaacatac gtgatcagct gattgaagct tacccagaaa
    39301 aacaggaagt atggacaaaa aatgcagcag cctataccga gaaacttcaa gcactccatc
    39361 agctttatca agaaaccttt aagcaagcaa aacaaagatc ttttgtcacg caacatgctg
    39421 cattcaacta tctagcgtta gaatatggat tgaatcaagt atcgattgct ggtctttctt
    39481 ctagtgaaga accgtctgcg gcgagaattg ctgaactgaa acactttgtt aaagaacatg
    39541 gaatcaacta tatttatttt gaagaaaacg ccaaagatag tattgccaga acgttagcaa
    39601 acgaagcagg tgtatcttta gaagtattga atcctttgga aggattgacg aatgaacaaa
    39661 tagaaaatgg agaaaactat ctttctatca tggaagcaaa cctcgaagcg ttgaaaaaaa
    39721 cgacagagac agaaaacccg ctagaagata ctttgacccc caaaaaagaa aagagcgtct
    39781 acaacggtta ttttgaagac gaggatgtac aagatcgctc tctttcagat tgggcaggaa
    39841 cttggcaaac agtagatacg tatgtagaag atgggacttt tgatcctgtt tttgaatata
    39901 aagaaaaatt gaatcaggat aagacagcac aagaatataa agaatattat acgaagggct
    39961 atcaaacaga tattgcttca atcaagatca gtgagaaaga gatggcattt acgtttaaga
    40021 atggaacaac gaaacgttca aattatcgtt atgtagggaa agaaatcttg acctacgagg
    40081 caggaaaccg tggcgtaagg tatctattcg aagcagaaga taaggaaagt ggcgctttta
    40141 gatatattca attcagtgat cacacgattg caccggctga ctccgaacat tttcatttat
    40201 atgcaggaaa tgaaagtcaa gaagtattgc tgaaagaaat ggagaattgg ccaacatttt
    40261 atccagatac aatggatgga cgtgaaattg cacaggaaat gcttttgcac tgataggcaa
    40321 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
    40381 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
    40441 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
    40501 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
    40561 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
    40621 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
    40681 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
    40741 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
    40801 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
    40861 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
    40921 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
    40981 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
    41041 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
    41101 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
    41161 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
    41221 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
    41281 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
    41341 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
    41401 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
    41461 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
    41521 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
    41581 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
    41641 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
    41701 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
    41761 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
    41821 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
    41881 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
    41941 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
    42001 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
    42061 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgc
    42121 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
    42181 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
    42241 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
    42301 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
    42361 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
    42421 gatttttacc aaattatagc acaattggcg tattattttt ttaatttttc agggattttg
    42481 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
    42541 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
    42601 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
    42661 agctgagaaa aataacgtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
    42721 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
    42781 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
    42841 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
    42901 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
    42961 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
    43021 tagttttcca agattattca ataaaacttc taagacaaat tttacttgga catacaccat
    43081 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
    43141 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
    43201 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
    43261 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
    43321 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
    43381 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
    43441 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
    43501 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
    43561 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
    43621 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
    43681 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
    43741 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
    43801 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
    43861 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
    43921 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
    43981 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
    44041 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
    44101 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
    44161 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
    44221 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
    44281 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
    44341 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
    44401 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
    44461 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
    44521 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
    44581 tacactacaa ataacccgaa agcaaaggga atctttacaa ctaaataata taaaaactaa
    44641 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
    44701 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
    44761 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
    44821 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
    44881 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
    44941 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
    45001 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
    45061 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
    45121 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
    45181 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
    45241 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
    45301 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
    45361 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
    45421 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
    45481 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
    45541 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
    45601 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
    45661 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
    45721 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
    45781 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
    45841 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
    45901 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
    45961 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
    46021 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
    46081 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
    46141 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
    46201 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
    46261 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
    46321 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
    46381 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
    46441 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
    46501 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
    46561 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
    46621 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
    46681 aaaactcttt tcttattaac taattatcgt tctcgggcag ctattgtcca aggagcaaat
    46741 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
    46801 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
    46861 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
    46921 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
    46981 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
    47041 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
    47101 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
    47161 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
    47221 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
    47281 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
    47341 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
    47401 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
    47461 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
    47521 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
    47581 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagta
    47641 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
    47701 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
    47761 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
    47821 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
    47881 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt
    47941 gacaagaatt ttgtcacacc tcccttattt attttgaaaa atgagaatac tattatcaaa
    48001 atagagaata tatagaaaaa ttctcattat tcaaacgtaa ataatcagtt attttgattt
    48061 tttttatatt tta
//
LOCUS       DASJTM010000010        48000 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJTM010000010 DASJTM010000000
VERSION     DASJTM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.010"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            228..599
                     /locus_tag="U4B29_000538"
     CDS             228..599
                     /locus_tag="U4B29_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2105508.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(873..1565)
                     /locus_tag="U4B29_000539"
     CDS             complement(873..1565)
                     /locus_tag="U4B29_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2105509.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1568..2353)
                     /locus_tag="U4B29_000540"
     CDS             complement(1568..2353)
                     /locus_tag="U4B29_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2105510.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2590..3990
                     /gene="lacG"
                     /locus_tag="U4B29_000541"
     CDS             2590..3990
                     /gene="lacG"
                     /locus_tag="U4B29_000541"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN2105511.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4015..4332
                     /locus_tag="U4B29_000542"
     CDS             4015..4332
                     /locus_tag="U4B29_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2105512.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4345..6018
                     /locus_tag="U4B29_000543"
     CDS             4345..6018
                     /locus_tag="U4B29_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN2105513.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6032..7663
                     /locus_tag="U4B29_000544"
     CDS             6032..7663
                     /locus_tag="U4B29_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN2105514.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8154..9719)
                     /gene="guaA"
                     /locus_tag="U4B29_000545"
     CDS             complement(8154..9719)
                     /gene="guaA"
                     /locus_tag="U4B29_000545"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2105515.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9778..10701)
                     /locus_tag="U4B29_000546"
     CDS             complement(9778..10701)
                     /locus_tag="U4B29_000546"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN2105516.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10897..11502
                     /locus_tag="U4B29_000547"
     CDS             10897..11502
                     /locus_tag="U4B29_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2105517.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11810..12826
                     /locus_tag="U4B29_000548"
     CDS             11810..12826
                     /locus_tag="U4B29_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2105518.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12967..14268
                     /locus_tag="U4B29_000549"
     CDS             12967..14268
                     /locus_tag="U4B29_000549"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN2105519.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14314..14976
                     /gene="deoC"
                     /locus_tag="U4B29_000550"
     CDS             14314..14976
                     /gene="deoC"
                     /locus_tag="U4B29_000550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN2105520.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14997..15395
                     /locus_tag="U4B29_000551"
     CDS             14997..15395
                     /locus_tag="U4B29_000551"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN2105521.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15461..16543
                     /locus_tag="U4B29_000552"
     CDS             15461..16543
                     /locus_tag="U4B29_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2105522.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16755..18317
                     /locus_tag="U4B29_000553"
     CDS             16755..18317
                     /locus_tag="U4B29_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105523.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18310..19443
                     /locus_tag="U4B29_000554"
     CDS             18310..19443
                     /locus_tag="U4B29_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105524.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19443..20399
                     /locus_tag="U4B29_000555"
     CDS             19443..20399
                     /locus_tag="U4B29_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105525.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20498..21664
                     /gene="deoB"
                     /locus_tag="U4B29_000556"
     CDS             20498..21664
                     /gene="deoB"
                     /locus_tag="U4B29_000556"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN2105526.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21688..22506
                     /locus_tag="U4B29_000557"
     CDS             21688..22506
                     /locus_tag="U4B29_000557"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2105527.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22523..23230
                     /gene="deoD"
                     /locus_tag="U4B29_000558"
     CDS             22523..23230
                     /gene="deoD"
                     /locus_tag="U4B29_000558"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2105528.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23528..24214)
                     /gene="gpmA"
                     /locus_tag="U4B29_000559"
     CDS             complement(23528..24214)
                     /gene="gpmA"
                     /locus_tag="U4B29_000559"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2105529.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24386..24967)
                     /locus_tag="U4B29_000560"
     CDS             complement(24386..24967)
                     /locus_tag="U4B29_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2105530.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25100..25783)
                     /gene="rpiA"
                     /locus_tag="U4B29_000561"
     CDS             complement(25100..25783)
                     /gene="rpiA"
                     /locus_tag="U4B29_000561"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN2105531.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26102..26515
                     /gene="rpsL"
                     /locus_tag="U4B29_000562"
     CDS             26102..26515
                     /gene="rpsL"
                     /locus_tag="U4B29_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN2105532.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26599..27069
                     /gene="rpsG"
                     /locus_tag="U4B29_000563"
     CDS             26599..27069
                     /gene="rpsG"
                     /locus_tag="U4B29_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN2105533.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27163..29250
                     /gene="fusA"
                     /locus_tag="U4B29_000564"
     CDS             27163..29250
                     /gene="fusA"
                     /locus_tag="U4B29_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN2105534.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29422..30609
                     /gene="tuf"
                     /locus_tag="U4B29_000565"
     CDS             29422..30609
                     /gene="tuf"
                     /locus_tag="U4B29_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2105535.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31016..31324
                     /gene="rpsJ"
                     /locus_tag="U4B29_000566"
     CDS             31016..31324
                     /gene="rpsJ"
                     /locus_tag="U4B29_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN2105536.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31355..31984
                     /gene="rplC"
                     /locus_tag="U4B29_000567"
     CDS             31355..31984
                     /gene="rplC"
                     /locus_tag="U4B29_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN2105537.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32012..32635
                     /gene="rplD"
                     /locus_tag="U4B29_000568"
     CDS             32012..32635
                     /gene="rplD"
                     /locus_tag="U4B29_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN2105538.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32635..32925
                     /gene="rplW"
                     /locus_tag="U4B29_000569"
     CDS             32635..32925
                     /gene="rplW"
                     /locus_tag="U4B29_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN2105539.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32964..33797
                     /gene="rplB"
                     /locus_tag="U4B29_000570"
     CDS             32964..33797
                     /gene="rplB"
                     /locus_tag="U4B29_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN2105540.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33840..34118
                     /gene="rpsS"
                     /locus_tag="U4B29_000571"
     CDS             33840..34118
                     /gene="rpsS"
                     /locus_tag="U4B29_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN2105541.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34141..34488
                     /gene="rplV"
                     /locus_tag="U4B29_000572"
     CDS             34141..34488
                     /gene="rplV"
                     /locus_tag="U4B29_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN2105542.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34502..35158
                     /gene="rpsC"
                     /locus_tag="U4B29_000573"
     CDS             34502..35158
                     /gene="rpsC"
                     /locus_tag="U4B29_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN2105543.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35161..35595
                     /gene="rplP"
                     /locus_tag="U4B29_000574"
     CDS             35161..35595
                     /gene="rplP"
                     /locus_tag="U4B29_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN2105544.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35585..35773
                     /gene="rpmC"
                     /locus_tag="U4B29_000575"
     CDS             35585..35773
                     /gene="rpmC"
                     /locus_tag="U4B29_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN2105545.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35798..36064
                     /gene="rpsQ"
                     /locus_tag="U4B29_000576"
     CDS             35798..36064
                     /gene="rpsQ"
                     /locus_tag="U4B29_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN2105546.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36122..36490
                     /gene="rplN"
                     /locus_tag="U4B29_000577"
     CDS             36122..36490
                     /gene="rplN"
                     /locus_tag="U4B29_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN2105547.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36527..36835
                     /gene="rplX"
                     /locus_tag="U4B29_000578"
     CDS             36527..36835
                     /gene="rplX"
                     /locus_tag="U4B29_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN2105548.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36863..37402
                     /gene="rplE"
                     /locus_tag="U4B29_000579"
     CDS             36863..37402
                     /gene="rplE"
                     /locus_tag="U4B29_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN2105549.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37421..37606
                     /locus_tag="U4B29_000580"
     CDS             37421..37606
                     /locus_tag="U4B29_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN2105550.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37642..38040
                     /gene="rpsH"
                     /locus_tag="U4B29_000581"
     CDS             37642..38040
                     /gene="rpsH"
                     /locus_tag="U4B29_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN2105551.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38072..38608
                     /gene="rplF"
                     /locus_tag="U4B29_000582"
     CDS             38072..38608
                     /gene="rplF"
                     /locus_tag="U4B29_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN2105552.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38769..39125
                     /gene="rplR"
                     /locus_tag="U4B29_000583"
     CDS             38769..39125
                     /gene="rplR"
                     /locus_tag="U4B29_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN2105553.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39146..39646
                     /gene="rpsE"
                     /locus_tag="U4B29_000584"
     CDS             39146..39646
                     /gene="rpsE"
                     /locus_tag="U4B29_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN2105554.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39661..39840
                     /gene="rpmD"
                     /locus_tag="U4B29_000585"
     CDS             39661..39840
                     /gene="rpmD"
                     /locus_tag="U4B29_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN2105555.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39884..40324
                     /gene="rplO"
                     /locus_tag="U4B29_000586"
     CDS             39884..40324
                     /gene="rplO"
                     /locus_tag="U4B29_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN2105556.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40324..41619
                     /gene="secY"
                     /locus_tag="U4B29_000587"
     CDS             40324..41619
                     /gene="secY"
                     /locus_tag="U4B29_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN2105557.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41681..42328
                     /locus_tag="U4B29_000588"
     CDS             41681..42328
                     /locus_tag="U4B29_000588"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN2105558.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42519..42737
                     /gene="infA"
                     /locus_tag="U4B29_000589"
     CDS             42519..42737
                     /gene="infA"
                     /locus_tag="U4B29_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN2105559.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42770..42886
                     /gene="rpmJ"
                     /locus_tag="U4B29_000590"
     CDS             42770..42886
                     /gene="rpmJ"
                     /locus_tag="U4B29_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN2105560.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42904..43269
                     /gene="rpsM"
                     /locus_tag="U4B29_000591"
     CDS             42904..43269
                     /gene="rpsM"
                     /locus_tag="U4B29_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN2105561.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43297..43686
                     /gene="rpsK"
                     /locus_tag="U4B29_000592"
     CDS             43297..43686
                     /gene="rpsK"
                     /locus_tag="U4B29_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN2105562.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43766..44704
                     /locus_tag="U4B29_000593"
     CDS             43766..44704
                     /locus_tag="U4B29_000593"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN2105563.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44732..45112
                     /gene="rplQ"
                     /locus_tag="U4B29_000594"
     CDS             44732..45112
                     /gene="rplQ"
                     /locus_tag="U4B29_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN2105564.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45384..46223
                     /locus_tag="U4B29_000595"
     CDS             45384..46223
                     /locus_tag="U4B29_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2105565.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46199..47068
                     /locus_tag="U4B29_000596"
     CDS             46199..47068
                     /locus_tag="U4B29_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2105566.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47058..47855
                     /locus_tag="U4B29_000597"
     CDS             47058..47855
                     /locus_tag="U4B29_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2105567.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15178 a         8773 c        10519 g        13530 t
ORIGIN      
        1 tctagagttt ctaaagtttg tttcttaatt agcttattga ttctataata tttttgattt
       61 atataggaaa ttaaaaatat tatagaatca acactcagag aacgggttgg tttttaatag
      121 atttaataag tactgataat gaaatttctc ctggttggga aaacgaagtt ggttaccctg
      181 tagaagaatt ttttcaagat tactttgcgg agtatgccga taggataatg agagattacg
      241 taacagataa aactcaagaa gtcatcaatc aatcccagtc attaaatgaa ttatctgata
      301 aggcgaaaga agaagcagtt gccattattt cttcagggat tagtgactat attgatatga
      361 gtggtgaaat caattatggc gataaagaca aaattgtacg catgataaaa gcttttatca
      421 aaacttttga tgtgaaggca gatcttaagt ttaatgatac ttacctagta ggaaaattaa
      481 ttaatatcct tgggacagag aaagatacgg aaacgtttat ttcctctttg tctatgttgt
      541 ttacagataa gcatttttca actcagcttg gtggagagga actagtggaa atcttttaac
      601 cattacgccc agcattaata aaagtatatc aaaaaagtac tcctgatgat ttttaagatt
      661 ggtttgagaa ataatgtgaa aacactattt ttaatgttaa ttatagatag agatgaacgc
      721 gtcatatggt taaaagaatg aaaaagtcaa agatacactg ctatatttca ctaaggatat
      781 agcagtgtat ctttcatgtc gtttcactgg cagtttttta taaaaggagg tgtgcatttc
      841 agttgctttt ctttggtagt tagttgaatc gtttaagaat taacggatgt ggcagacagg
      901 tgctctgtcc aaaaaccagt aaaatctttg atggttccat ctttatttag ctgtgcctgt
      961 tcaaatactg ttttgttttt accgaatgga tcaattgcaa taattgttcc gatccccgca
     1021 cgtttggcag caagtaatcc tgagtaagca tcttcgataa caagacaatc agccggagct
     1081 aagctcagtt tgtctgccgc tttaagaaaa atatccggtt gcggtttccc agggaaagag
     1141 ccgtcgtcat agacaatctt gtcccagtca aaccagcggg ctaaatcgaa aatatcaaaa
     1201 taaaaagcaa cattctcttt gacagtagcc gtggcaatcg ttaatggaat ctgttggtca
     1261 gtcagctggt caagagtcgc ttcaagccct tttgtcaact gaagctcgtt ttcattttgc
     1321 aagcaaagca tacgataata gctttctttt tcttctgaca atacggcgat ctcagaggcg
     1381 gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc atgaatattt
     1441 tttaaaatat cctcgtctga taaaccatct tttgtatgat catgtatcat atgcagccaa
     1501 gcagcttcat gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa tataatccct
     1561 ttcatagtta cgctcctata gtggtatttt cggttgaatc agcaatttgc agatcgacat
     1621 attgtgcata gtgctcttga acctcccgcg agatctggcg atccgtaatg agcagatcga
     1681 aatcatgcaa attataaaac acataaaaat catctcgatt gaatttttta ttatcaacga
     1741 gtagatattt cttacgggaa ttatttagag cgatctgctg tgcttctcct tcttcggtac
     1801 tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact gataaatgct tttgtaaact
     1861 ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta tttttccgga
     1921 aatcaccgcc aatcagtaga atttctggtc catctgcttg agcaagaata tcaaaaactg
     1981 gataattatt tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc aatagttcga
     2041 ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag gctgctgtgg
     2101 cagcaatttc ttttttttct tgaatctgca gagtggcttt ttcagtgtga gaaagttctt
     2161 gatctaaagt gtaggaaaga ctttgagccc ctccatggac acgcagcagc ttgcccgatt
     2221 tttctaattc atctaaatct ctgcgaacgg tcatatcaga aacatctaat tgattgatga
     2281 tttcattaac ggtaaggatt ccttttttat tgaccatttc tacgatagta agcaatcttt
     2341 cttttttcaa cattgtcttc acctctaatt taaggataat ctatcatata aacggacaac
     2401 agtcaatcta attcgaacaa aagtaaacat ttagggtggt ttttatgttt gttacaatag
     2461 attttattgc tgttttctgg tttttatgat tatttgtgtt tgtttttgtt gacaaatgtt
     2521 ttggatgcgt ttacaatgca atcagttaga aagagcgaaa actgattata gatttgagga
     2581 gagatgtaca tgcttaaatt accagaagat tttatttttg gcggagccac agcagcctat
     2641 caggttgaag gagccactaa ggaaggtggc aaaggcgctg ttgcttggga tgacttttta
     2701 gaggaacaag ggcgattcag tcccgatccg gcaagtgatt tttatcatca atatgcaaag
     2761 gacattgaat tgtgtgaacg ttttggtgta aatggtctaa gattatccat tgcttggagc
     2821 cgcatttttc ctgatggagc tgggaaacca aatccagaag gaatcgcttt ttatcatcgt
     2881 gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg tgacgttgca tcattttgat
     2941 acccctaaac gtttgtttga ccatggagat tttttgaatc gagaaacaat agaagcattt
     3001 gtttcctatg ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg gagtaccttt
     3061 aatgaaatct atccagttgc aaccaatcaa tatttgttag gtgttttccc accaggaatc
     3121 aaatatgatt ttacaaagat cgtcgcctgt cttcataata tgatggttgc tcatgcccgt
     3181 gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa ttggtgtagt tcactcatta
     3241 gagacgaaat atgcggcaac agacgcgccg gaagataagc atgctgcctt tttagacgat
     3301 gcattgtcga tccgtttctt attagatgct acctatttag gctattattc aactgaaacc
     3361 ttgactgctc ttgatgagat atgtgaagca aatcaggcca gctatcattt tcctgaagaa
     3421 gatttcgttg aattgaaaaa agcatccacg cgtaatgact acttagggat caatcactac
     3481 caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa tccatcataa cggtacgggt
     3541 gaaaaaggga cctctgttta taaagtgaag ggaatcggag aacgtattta taaagaagga
     3601 attcctcgaa ctgattggga ctggctgatt tatccagaag gtctatatga cttgttgctg
     3661 cgaattaaaa gcgattaccc acattacaac aaaatctata tcactgagaa cgggatgggg
     3721 tataaagatc agtttgaaga tggcattatt atggatcagc cgagaatcga ctatttgaga
     3781 gtctatttgg aatccttaag caaggcgata acagccgggg taaatgtgaa ggggtatttt
     3841 ttgtggtcgc ttatggatct tttctcatgg acaaatgggt ataacaaaag gtatggcttg
     3901 ttttatgttg attttgaaac ccagaagcgt tacccaaaag aatcagctta ctggtacaag
     3961 ttggtcagtg aaaccaaaac gattatttaa taagtgataa tggaggaaga aaagatgaat
     4021 aaagaggaat tacaaatgct tggttttgag atcgttgcat attcaggcga cgctcgcagt
     4081 acattattga aattattgaa agaagtaaga caaggaaact ttgaacatgt tgattcggcg
     4141 ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt cgcaaacaaa aattttagcg
     4201 gaagaagcat ctgggaaaga gatggagatg ggattcattt ttatccatgg gcaggatcat
     4261 ttaatgacca ctttattgct gagagatctg attcaagact ttattgtact gtacagacag
     4321 aatcaaggat aggagaagaa cgctatggat ggaatcatta aacaaatcga aaaagggaaa
     4381 ccattttttg aaaaaatctc acgtaatagt tatttaggag ccgttcgtga cggattttta
     4441 acagcgatgc cagctatttt attttcaagc attttcattt tgattgctgc ggttcctgag
     4501 atttttggtt accaatggcc tgaatctgtc tccacatggc tatggaaagt ctataattat
     4561 tcgatgggtg ttgtagggtt actcgtttca gcaaccactg ctcgctgctt ggctgattcg
     4621 atgaatcgtc gcttaccgaa aaacaaagta atcaacacca cctctgtgat gctggcctct
     4681 gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag gtggattttc aacggaatat
     4741 atggggacaa agggactact ggcttccttt gtcgctgcat ttttaacagt taatttatat
     4801 aagttttgtg tgctgaaaga tattaccatt aaaatgccta aagaagttcc tggaacgatt
     4861 tcgcaaacct tccgagatat ttttcctttc tctttttcag tatttgcggc cgttttaatt
     4921 gatttaggca ttcgacaaat cttccaaatc tcatttgcag aagcaatcat tacggtgctg
     4981 cagccgctat ttacagcagc tgacgggtac ttaggaatcg ctattatctg gggagcgatg
     5041 gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg tggagccggc aattgcggca
     5101 attatctatg ccaatgtgga aaccaatctg caattgttca attccggtga acaagcttct
     5161 aaagtactga cggtgggttt agggaatttt gtaggaacta tgggaggaac tggggcaaca
     5221 ttggttgttc cttttatctt cctactcttt gctaagtcta agcagttgaa ggcagttggg
     5281 aaagcttcct ttattccagt aagtttcgct gtcaacgaac cattgctatt tgcggcaccc
     5341 atgattttga acccttattt ttttattcca tttttgttaa cacctgttgt aaacgtttgc
     5401 ctgttcaagt tttttgtcga tgtattgggc atgaacagct ttatgtacgt tttgccttgg
     5461 gcaactccag caccgatcgg tttggttcta ggaacgggca tgggtatctt agcttttgtt
     5521 ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc taccattttg caaagcatac
     5581 gatgctgtct tagtagagaa agaagcacaa aaagcagagg tgatcgcaac cgaggaaggt
     5641 gcttctgccg atgtacgtga ggaagtacct gtggcagcga gtcaagttat cagcgatgca
     5701 aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag ggacaagtgg gatgttagct
     5761 aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga tcagtgcaat ggcgggtgct
     5821 tatggttctc attacgaaat gctgcaggat tttgatatgg tggtcttagc accgcaagtc
     5881 aattcttact atgatgatat caaagaagac accgatcgct taggaatcaa gctggttgcg
     5941 acaaaaggag cagaatatat cggattaacg agagatcctg aaaaagctgt tcagtttatt
     6001 ctgaaccaat ttcaataaag gaggagccat aatgaaaatg ggaatttggt tgcgtaaagc
     6061 tgttttattt gcagctgtgt taggggcttt gggggctgtt tcaaaaactg ttgaagcatc
     6121 tgccagcgaa tttattcgcg gagcagatat ttcgatcctg tcagatatgg aaaaatctgg
     6181 tgcaaaatat tatgaagacg atatttcaaa agatgcctta gagattttaa aaaataatgg
     6241 tgtcaactat gttcgactgc gtttgtggca ggatccttat aatagtaatg gagaaagtta
     6301 tggagcaggg acaaatgatc tggaaacgac gattgacctt gcaaaacgag caaaaaatct
     6361 gggacttaag gtgctgctgg attttcacta cagtgatttt tgggttgatc ctgggaaaca
     6421 gaatcttcct aaagcatggc aaggtctgac ctttgaagaa atgaatacgg cattgtacga
     6481 ctatacaaag aacgttctgt cggaaatgaa gcagctggac gtttatcctg atatggttca
     6541 gatcggcaat gaactgaata gcggaatgct ctggccttat ggcaagtctt ggggagaagg
     6601 cgggggagaa tttgatcgat tggctgcttt tttgaagtct ggtatccaag ctgttcgaga
     6661 cactcaacct aaaacaactc cagtaatgct ccacttagct gatggtggcg atacaggagc
     6721 atttacttgg tggtttgatg aaatcacgag tcgaggtgtg tcatttgatt taattggagt
     6781 gtcgtattat ccgtattggc acggttcaat gaatgaactt caaaccaata tgaatgcgat
     6841 cagccaacgg tatggtaaag aagtgatcgt agttgaaaca gcttatgggc atacaacagc
     6901 gaatgccgat actatgccaa atgcttttgg tgaagccgaa gcagctgcgg ggggatatga
     6961 accaacaccg acaggacaag cagagtattt gctggatctt gccgatcgga ttcaggcagt
     7021 accgaataat cgcggggcgg gcttcttcta ttgggaacct ctttggtaca acggaaatgt
     7081 gagttgggca acacaagcag gtatgaatta tctcagtgtt caatccacta tgggaaacga
     7141 atgggacaac caagcgatgt ttgattttgc tggaaacgcc ttgccaagct tgcgtgcgtt
     7201 taaacaagca ggggcgcaaa taaatctagt taaaaatgca agtttcgaaa gcgatggacc
     7261 aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa ggaaccgcta agggtacgat
     7321 caaaacggaa gcgggcactt tttttgggga ctacaaacta actttttggc aggatcaagc
     7381 atacgaggca tcagcttatc aaaatgttag tggcataagc aaaggaactt acacattgag
     7441 tgcctatgta atgtctgggg gagagtttga tactaatcag ttatatatca aaaattatgg
     7501 tggagcggaa ctaaatactg cggttgttaa aacaagtgaa tggacaaaaa ttacaatcaa
     7561 agacattcca attacaacag gtgtttgtga aattggggtt tatagcaagg cttcaggaaa
     7621 tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa tagactgcgg atcgtttcga
     7681 ttcaagatcg gaggttccct tcagccacat cgttagcgta gaaggcagga atcgaacctg
     7741 cacctcgcct ttgccaaaaa taaccagaaa cgatttccag tcatttttac caaaacgagt
     7801 aaagcaccag acttcatcta cactttgtat tatgctaaag agagttcaac gatttcgtaa
     7861 gaagcaatga aaaaattgca aaaagttgtg cagataacat aaagtttgtt taagaaaggg
     7921 taaccttaga attccgtcgt gaatatgatt tgagcactta gatggataga aaaaagttct
     7981 cggaaaacag ttcgtttttc gagaacttct ttttatgata agaatgaaac aaattaactt
     8041 atattatagt ctcttttggt agggaaccat cttattttta atgaaaatca agcattttag
     8101 ctattgaaga atgttgattt atagggattc cccctccata ttatgttggc tctttattcc
     8161 cactccactg ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct
     8221 acttcgttta cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag
     8281 tcagcggtca ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta
     8341 cgtccgtcac ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag
     8401 atatcacggt caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt
     8461 acgatttcta gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca
     8521 gggaatggct gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg
     8581 acttcgtctt tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc
     8641 agtccaccta cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata
     8701 acgtcagtat acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt
     8761 gttgcttcgt catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct
     8821 gggtcagata cacctttcaa cttgtctaag aagcgatctc ttgcattgac tttgatgatg
     8881 ttcaagccga attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc
     8941 agaccgtggt caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc
     9001 ccaacgacac tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact
     9061 tgttcacgga ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca
     9121 cagtgacaaa catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt
     9181 acttctgcat ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc
     9241 ggacaatctt tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca
     9301 ccatggctca tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg
     9361 cttgttactt ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg
     9421 ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa
     9481 ttgaagatct caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca
     9541 gaaagaatga ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc
     9601 aataattcgg aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga
     9661 ctaccgtagt ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa
     9721 tttattaccc ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta
     9781 atattttcgc atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc
     9841 ggctcttcct cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca
     9901 tacatctttt gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata
     9961 attctctgga ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt
    10021 ttcgatcagc acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta
    10081 gatttgctga ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg
    10141 ctgctggatc aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg
    10201 tacttttatc tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat
    10261 atcataactt tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt
    10321 tggataaaga aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga
    10381 caaaatcgtc tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat
    10441 cccgataata aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa
    10501 caaagattca aattctttca tataaagata aatcagatga gcaagaggta catagatatc
    10561 tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg
    10621 tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta
    10681 gtagttcatt ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta
    10741 acacaaaata aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct
    10801 ttataataga aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag
    10861 actgttgtca tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa
    10921 cgcccagata cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt
    10981 caatttgtaa cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc
    11041 ctgattgatg ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga
    11101 tgcgggtatg gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg
    11161 gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc
    11221 gaacatgttg atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca
    11281 gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca
    11341 ggcgcatatc ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa
    11401 ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca
    11461 aaagataaag gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg
    11521 agcgacgaaa gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt
    11581 gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt
    11641 cgttgttttt tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg
    11701 gtagataaag gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg
    11761 ggggttttgg tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac
    11821 catcagagat atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa
    11881 taacaaagga agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga
    11941 acatcattat aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat
    12001 cggaatgatc gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga
    12061 gagctatttg aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga
    12121 aaatgagatc aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc
    12181 cacaccaaat attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc
    12241 aatcatttta atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga
    12301 atatgagggc gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg
    12361 catgctgaag gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca
    12421 tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac
    12481 tgtccaaggt ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat
    12541 tttttgcggg aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa
    12601 aaaaatccca gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat
    12661 gattccgcaa ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg
    12721 tttcctgatc gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac
    12781 aaaattaatt attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt
    12841 gcttatgtaa gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt
    12901 actaaagtgg tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat
    12961 caggcaatgc gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa
    13021 gaggaaatcg agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc
    13081 agtgcattat tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta
    13141 acattggcga ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag
    13201 gtagataagc attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta
    13261 gtagctagtg tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt
    13321 ggtacactag acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa
    13381 tttgtacgca ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca
    13441 ccggcggaca aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg
    13501 ctgattgcca gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg
    13561 gatgtgacca ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca
    13621 aagaccatga cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat
    13681 atgtcagaac ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa
    13741 acattacaag gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa
    13801 atggttgttc ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa
    13861 gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga
    13921 gacccagcga tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt
    13981 cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca
    14041 atgatgctag gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg
    14101 aaactgcata aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat
    14161 agtaacgacg aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt
    14221 gaatcggcaa tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg
    14281 gactttaccg ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat
    14341 actattttga aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa
    14401 aaataccatt tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa
    14461 ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca
    14521 cccgagacaa aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat
    14581 atggtcatca atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc
    14641 gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg
    14701 ttattagata gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat
    14761 tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg
    14821 atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa
    14881 gaagaagcaa tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta
    14941 gcaatcgtat ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg
    15001 cagaactcaa acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc
    15061 cttattccca ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag
    15121 gcatcaacat tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt
    15181 tcaaagcagt atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc
    15241 ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg
    15301 atatgccggt tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac
    15361 tgctgcctta tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact
    15421 gttgaaaaat aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt
    15481 tggtttaggt gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg
    15541 ctctactgat tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac
    15601 agacacaggt ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa
    15661 atgggggaaa gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc
    15721 aaatgaatca gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat
    15781 ttttggtatt ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg
    15841 tacgaacttt gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac
    15901 tttcaaagac aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac
    15961 aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg
    16021 attcaaagcc ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa
    16081 tcaatatgct ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta
    16141 tgcacaaaac gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca
    16201 agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg
    16261 cgaccaatca gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac
    16321 atctacactg aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg
    16381 taaattccct ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac
    16441 agaaggacaa ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat
    16501 ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct
    16561 tgttagacac atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc
    16621 aagctgcctt gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa
    16681 agaagaacgc tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag
    16741 gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag
    16801 aatttggaac attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc
    16861 atgcgttgct tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat
    16921 tattagagcc gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc
    16981 cgaccaaagc caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg
    17041 cctttactgt aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg
    17101 accataaaaa agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca
    17161 acccagatgc ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa
    17221 aaacacttta ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc
    17281 ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga
    17341 tcattttgat tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta
    17401 tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag
    17461 ccgatatgat ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc
    17521 aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg
    17581 tgaaaaactt gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg
    17641 gaaacgggca gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac
    17701 atatcaagct aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg
    17761 gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat
    17821 ctgaaaatat cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga
    17881 attattcagt aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa
    17941 cgaatgaact tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg
    18001 ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg
    18061 atgtaggtgc gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag
    18121 cagtgttact gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg
    18181 ttatccatga aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat
    18241 taggtctatt gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg
    18301 ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca
    18361 gttgttttag gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc
    18421 caaggttatt cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg
    18481 ttaaggcaag caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct
    18541 gggtttttca atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg
    18601 gtggcacttg cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta
    18661 ggtgccttag ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga
    18721 acaagtgaag tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt
    18781 gctttacatg aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc
    18841 agtcaaaatg cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta
    18901 aactcaggat tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa
    18961 acgactttag gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca
    19021 ggaatgagta gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg
    19081 cttggcggtg tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg
    19141 ctatctattg gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt
    19201 attcttttat cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca
    19261 ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc
    19321 gggatcaact tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa
    19381 gagatcgttg cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta
    19441 taatgtcgac tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac
    19501 cgctaattct aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag
    19561 ggctagaggg aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga
    19621 gtccatcact tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta
    19681 tcctattttc tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca
    19741 gcgggacagt aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt
    19801 atggtaaagg tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat
    19861 tagcagatat tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt
    19921 gtgctatttt gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc
    19981 gcctccgttc agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg
    20041 ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt
    20101 ttgctcagtc gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct
    20161 ccatggctgc tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt
    20221 tctttggttt tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga
    20281 ttccgccagt attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt
    20341 tcttagggaa agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat
    20401 cttttagcga ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt
    20461 taaaggcaat aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat
    20521 cgtcatggat tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg
    20581 ttctgataca ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca
    20641 gcttggttta ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg
    20701 ctatgcgacg aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga
    20761 aatcatggga ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga
    20821 tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag
    20881 cggaacagct gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt
    20941 atatacttct gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga
    21001 agaattatat cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat
    21061 tggccgaatc attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa
    21121 tcgccatgat tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc
    21181 aggaaaggac gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac
    21241 tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa
    21301 aaacgatttt tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca
    21361 tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga
    21421 attgcttgaa gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga
    21481 tccgacgttt actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa
    21541 aaaaatgaaa gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat
    21601 tgctgagaac tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa
    21661 ataatctttt taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg
    21721 acccagttta tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca
    21781 ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc
    21841 ccgttctttc ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg
    21901 gcaggcaaga aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg
    21961 caaacagtga cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg
    22021 actaatgcat gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc
    22081 gatcatatca attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc
    22141 cctagatttc cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca
    22201 gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca
    22261 acttatgaaa cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg
    22321 atgtcgactg ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc
    22381 tcttgtatta ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta
    22441 gaaacaactc agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta
    22501 ttgtaatagg aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc
    22561 cgataagatt ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt
    22621 ggaagatcca gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa
    22681 aggagaacgt atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc
    22741 ccatgaattg atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc
    22801 attgtcaaaa gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc
    22861 ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct
    22921 attactgaaa gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt
    22981 tctttcggaa gattctttct acaaagatga tttgacagag acgttccaat tagctgaact
    23041 tggcgtttta ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca
    23101 tgtgcagaca ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac
    23161 agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat
    23221 agcagaataa gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca
    23281 aaaatagctc ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga
    23341 acaccgttta ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt
    23401 caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa gttggcagga
    23461 gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc cttttttata
    23521 gtgtttttta taggtagtat tttttagcaa ctgttaagtc atcatttaat tcataaacaa
    23581 gtggttgacc tgttggaatt tcaagatcca tgatgtcttc gtcagagata ccttcgatat
    23641 gtttagctag ggcgcgtaat gagttaccat gtgcagctac taaaacagtt ttgttgtcta
    23701 gtaatgctgg agcaatttca tcttgccaga atggcaaagc gcgttctaat gtcactttca
    23761 agttttctcc accaggaata tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg
    23821 cagaaccttc gtcagtttct tccattaatg gaggaagagt atcgtaagaa cgacgccaga
    23881 tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt
    23941 taccatagtg acgttcgttt aagcgccatg atttgatttg tggtacccaa agttgatctg
    24001 aatattctaa gatcaagtta catgttttga ttgcacgagt taaaacagaa gtgtaagcaa
    24061 catcaaattc gattccagct tctttgattt tgcgtccgcc ttcttttgct tcttcgattc
    24121 cttctggtgc tagatttaca tcagcccaac cagtaaattg gttcaatgca ttccattcac
    24181 taagaccgtg acgagaaaaa actaattttg gcatgtttag ctctcctttt aagataggtt
    24241 tatatttctg aaattttcag ttctgtttta ttgtacaatt ttttggaaga gatttccatc
    24301 gaaaaattag ataagattct atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc
    24361 ccacgcaact gttcgttttt aggtattaga tgcataattg ctcgatttct ttttcaggta
    24421 aattgatata tttataacat gttccaactg aaacaccgca ttttgaagaa ataaattgga
    24481 tcgtttcttt tttttcataa tagagacgtc ggattttttt gatcgttttt gcatcgattt
    24541 ttggtcttcc gccgattttt ccttttttac gagcttcatc taatccaact aatgtacgtt
    24601 ctttgatcaa tgcgcactcc atgtttgcta ggttttccat taattggaaa tagatttttc
    24661 ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg cgatctttga
    24721 ataattgcgt gagttctgtt aattgccgcg tagatttgcc taatcgatga agttggcaaa
    24781 tgactagtgc atcgccttgt tgcatgtcag cgatcactga ctccaaatga acagcagtag
    24841 aatcattttg ttccacttca tatgtctcat ggaaaatttc ttgacaacca aagtcagata
    24901 attgatcaac ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca taaccaataa
    24961 ttttcatggt gattcctttc tagcagtgat tttctaactg gatataacag taaaaatgta
    25021 gcataaagta cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt tttttattgg
    25081 atgatcaagc agtttttctt tatttacgac ggattgcgtc aagaatctca acgccttttt
    25141 ctgagccgac aactacttta gaaaccatgt ttaggaaaag cccatgttct actaccccaa
    25201 caagttcatc taaataggta gctaagcttt ctggctgttc gatgacttct agatgcagat
    25261 caatgatgta atgaccacca tctgtggtta agacttctcc gttttcgtct gtacgcaaga
    25321 cgggttggaa tcttttttct tcaaaaatat gaagtagctg ttgggagcca taagggatca
    25381 cttcaactgg gagtgggaac ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc
    25441 aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc
    25501 ctttgatccc ttgaaaatca gcactgattt catcagcacc gtctattgtc aaatcgacta
    25561 cggggacttc atcaatactc tttaacggta ttcctaattt ttccgcttgt tcttttgtcg
    25621 catttgaagt agtaactcct gtgatacgta aaccttcttc acgcatacgg cgaccaatct
    25681 cttctaccat ataataggcg gttgaaccgg tcccgagacc tacgatcatc ccgtccttta
    25741 cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt catctttgcg tccacctttt
    25801 tttagtttga gcatcgtgct ttcctcacta ttttagcgtg atttacggta aaaagatacc
    25861 attctttttt attccaataa agctttttca aaaaaaattg catctcttgt tgacatgttt
    25921 tttcattcgt gatattctaa caaaggtgct ttatctacag gtttctgtga agaaacgtgc
    25981 tgactgttta tcaaagcata tttgataagg atgcagacat tgcatttatt ttttatcaca
    26041 aaaaaagaac cacctggatg tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg
    26101 aatgcctaca attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa
    26161 ttcacctgct ttgaacaaag gatataacag ctttaagaaa actcaaacta acgtaaactc
    26221 acctcaaaaa cgtggggtag caactcgtgt gggtacaatg acacctaaaa aaccgaactc
    26281 agctttacgt aaatatgcac gtgttcgttt gtcaaactta attgaagtta ctgcttatat
    26341 cccagggatt ggtcataact tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt
    26401 aaaagactta ccaggggtac gttaccatat cgttcgcggt gctttagata ctgctggtgt
    26461 taacgaccgt aaacaaagcc gttctaaata cggaactaaa aaacctaaag cttaatttta
    26521 attattaaac aaatgaaact tgcttctact atatagaagt aattactatg aacaatattt
    26581 cggaaggagg agttacggat gccacgtaaa ggtcctgttg caaaacgtga tgttttacca
    26641 gatcctattt ataactcaaa attagtaact cgcttgatca accgtgtaat ggttgacgga
    26701 aaacgcggga ttgcagctaa cattatctat aatgcctttg acttgatcaa agaatctaca
    26761 ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa
    26821 gttaaagcac gtcgtgttgg gggttcaaac taccaagtac cagttgaagt tcgccctgaa
    26881 cgccgcgtta ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac
    26941 acaatggaag aacgcttagc aaaagaaatc atggatgctg caaacaacac aggtgcttct
    27001 gttaaaaaac gtgaagacac tcataaaatg gctgatgcca accgtgcgtt tgctcactat
    27061 cgctggtaag attctctctg tttggtcggc ttttataagt cgacctcaga tagtgtcctt
    27121 atttttagca actagatagt aaagagagga gaaacaagga gaatgaccag agaattttct
    27181 ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg atgctggtaa aacgactgct
    27241 actgaacgta tcttgtacta tactggtaaa atccataaaa ttggtgaaac tcatgaagga
    27301 gcttcacaaa tggactggat ggaacaagaa caagaacgtg gtattacgat cacttctgct
    27361 gctactacag cacaatggaa gggtcaccga atcaacatca tcgacacacc aggacacgta
    27421 gacttcacag ttgaagttga acgttcattg cgtgtacttg atggtgctgt aactgttctt
    27481 gatgcacaat caggtgttga acctcaaact gaaacagttt ggcgtcaagc aacaacttat
    27541 ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga cttcttatat
    27601 tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc acccaatcca attaccaatt
    27661 ggtgcggaag acgacttcac aggaattatc gaccttgtaa caatgaaggc tgaaatgtat
    27721 acaaacgatc ttggaacaga gatcgaagaa acagaaattc ctgaagaata ccgcgaatta
    27781 gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg aaactgatga agaacttact
    27841 ttgaaatact tagaaggtga agaaatcact gaagctgagt taaaagaagg tatccgccgc
    27901 gcaacagtta acgtagaatt ctacccagta ctatgtggtt cagcatttaa gaacaaaggg
    27961 gttcaattac tattggacgc tgttcttgat tacttgccat caccgttaga tatcccagct
    28021 atcaaaggga ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga cgacagcgct
    28081 cctttctcag ctttagcatt taaagttatg actgacccat tcgtaggtcg tttaactttc
    28141 ttccgtgtgt actctggtgt tttacaatca ggttcatacg tacaaaacgc aacaaaaggc
    28201 aaacgtgaac gtgttggtcg tatcctacag atgcatgcca actctcgttc agaaatctct
    28261 gaagtttacg ctggagatat cgctgctgct gttggtttga aagacacaac tactggggac
    28321 acactatgtg atgaaaagag tctagttatt cttgaatcca tggaattccc agaaccagtt
    28381 atccaagtcg ctattgaacc taaatcaaaa gctgaccaag ataaaatggg tgtggcttta
    28441 caaaaactat ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa
    28501 acaatcatcg ctggtatggg tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt
    28561 gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga aactttccgt
    28621 gctggcactc aagccgaagg taagtttgta cgtcagtctg gtggtaaagg tcaatacggt
    28681 cacgtatgga ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg
    28741 atcgtcggtg gggttgttcc tcgtgaatac attccagctg ttgaaactgg tttgaaagat
    28801 gctatggaaa atggtgtctt agctggctat ccattagtag atatcaaagc taaactttac
    28861 gatggttcat accatgatgt cgattcaaat gaaactgcct tccgtgttgc tgcttctatg
    28921 gcattacgtg cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat catggctgta
    28981 gaagttgtta tccctgaaga ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt
    29041 ggacgtgttg aaggtatgga agctcgcgct ggcggccaac aagttgttcg tgcgatggtt
    29101 ccacttgctg aaatgtttgg ttatgctaca actcttcgtt ctgctactca aggtcgtggt
    29161 acatttacaa tgacatttga ccactacgaa gatgtaccaa aatcagtaca agaagaaatc
    29221 attaagaaaa acggcggaaa agctgaataa tttcttgaaa ccggatagtc ctgcaaagag
    29281 attatccaaa gagactatat ttttaatata gtttcgtgta aaataattat cgatgatatg
    29341 ggcatgtggt gtaccttgtg tacatcatat acctatcaga caaataaaaa atatattgta
    29401 tttttaggag gacattttaa aatggcaaaa gaaaaatttg accgttctaa accacacgtt
    29461 aacattggta ctatcggaca cgttgaccat ggtaaaacta cattaacagc tgcaatcaca
    29521 actgtactat ctaagaaaaa cggcggccaa gctatggctt acgatcaaat cgatggtgct
    29581 ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc acgttgagta tgaaacagat
    29641 acacgccact acgctcacgt tgactgccca ggacacgcgg attacgttaa aaacatgatc
    29701 actggtgctg ctcaaatgga cggagctatc ttggtagttt ctgctgctga cggcccaatg
    29761 cctcaaactc gtgaacacat cctattgtct cgtcaagttg gtgttcctta catcgttgta
    29821 ttcttgaaca aagtagacat ggttgatgac gaagaattac tagaattagt tgaaatggaa
    29881 gttcgtgacc tattaacaga atacgaattc cctggtgacg atgttcctgt agttgctgga
    29941 tcagctttga aagctctaga aggcgacgct tcatacgaag aaaaaattct tgaattaatg
    30001 gctgcagttg acgaatacat cccaactcca gaacgtgaca acgacaaacc attcatgatg
    30061 ccagttgaag acgtgttctc aattactgga cgtggtactg ttgctacagg tcgtgttgaa
    30121 cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga agaaacttca
    30181 aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt tagactacgc tgaagctgga
    30241 gacaacattg gtgctttact acgtggtgtt gcacgtgaag acatccaacg tggacaagtt
    30301 ttagctaaac caggtacaat cacacctcat acaaaattct ctgcagaagt atacgtgttg
    30361 acaaaagaag aaggtggacg tcatactcca ttcttcacta actaccgtcc acaattctac
    30421 ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag aaggaactga aatggttatg
    30481 cctggcgaca acgtaacaat ggaagttgaa ttaatccacc caatcgctat cgaaaacggt
    30541 actaaattct caatccgtga aggcggacgt acagttggtg ccggtgtcgt tacagaaatc
    30601 aaagcttaat tcacttaagt tttaaagtaa agcaacattt attcggacgt aatcgctcgg
    30661 aacgaaggtc ttaggatctt tgttccgagc ttttttattt attgcaggat caggaaaaaa
    30721 gacaggagta ttataaatag aagaaacacg aaataaaaga gtcagtattt tctaaatgaa
    30781 actttatgaa aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata
    30841 ttataatgac aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc
    30901 gtcggctaag aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac
    30961 ggaaattttc gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc
    31021 aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc
    31081 ggataaaatc gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt
    31141 accaactgaa cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg
    31201 cgaacaattc gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa
    31261 aactgttgat gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt
    31321 ataaaactaa aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa
    31381 agtgggaatg acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga
    31441 agctactcca aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat
    31501 tcaagttggt taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt
    31561 tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg
    31621 agaatatgaa gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga
    31681 tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag
    31741 ccgcggacca atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt
    31801 tgcacctaac cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt
    31861 aactatccaa aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa
    31921 aggtaatgtt cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa
    31981 ataattgtag gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga
    32041 tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga
    32101 aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc
    32161 ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac
    32221 aggtcgtgca cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt
    32281 tggaccaaca ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat
    32341 gaaatctgta ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa
    32401 cttcgacgca ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc
    32461 taaagtattg gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt
    32521 accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac
    32581 aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac
    32641 ttactagacg taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac
    32701 aagaaataca cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta
    32761 gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc
    32821 aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg
    32881 actgaagatt caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac
    32941 tcattaaatt aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc
    33001 gtcgtaacat gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat
    33061 tgttacaacc attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc
    33121 atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata
    33181 acgttgttgc aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt
    33241 tagtacacta tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg
    33301 gtatgaaact tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag
    33361 aaaacattcc agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac
    33421 aattgatccg ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat
    33481 tgatccgctt gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg
    33541 gttctgtagg taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt
    33601 ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg
    33661 gtggtggtga aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac
    33721 cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc
    33781 gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca
    33841 tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag
    33901 ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt
    33961 tcccaagttt tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt
    34021 acatccaaga agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc
    34081 gtggacatgc tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa
    34141 atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga
    34201 aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc
    34261 ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt
    34321 gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt
    34381 gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca
    34441 atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc
    34501 agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc
    34561 taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa
    34621 atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac
    34681 aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg
    34741 ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat
    34801 caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc
    34861 acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc
    34921 aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga
    34981 tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga
    35041 cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg
    35101 gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc
    35161 atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa
    35221 gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat
    35281 tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt
    35341 ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg
    35401 gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt
    35461 aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta
    35521 gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa
    35581 tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa
    35641 acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa
    35701 cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga
    35761 acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg
    35821 caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt
    35881 cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa
    35941 agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg
    36001 tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat
    36061 ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa
    36121 cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt
    36181 gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt
    36241 tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc
    36301 cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga
    36361 tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg
    36421 gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga
    36481 agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa
    36541 aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc
    36601 agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca
    36661 aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt
    36721 ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt
    36781 agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt
    36841 taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac
    36901 tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa
    36961 aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc
    37021 agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc
    37081 aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg
    37141 agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga
    37201 cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa
    37261 agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga
    37321 catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt
    37381 aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat
    37441 tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg
    37501 cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact
    37561 tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca
    37621 taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt
    37681 atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa
    37741 cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa
    37801 gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt
    37861 atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa
    37921 gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc
    37981 actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa
    38041 tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt
    38101 tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa
    38161 aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt
    38221 aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc
    38281 gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat
    38341 cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc
    38401 acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca
    38461 agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg
    38521 aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg
    38581 ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc
    38641 aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca
    38701 gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg
    38761 tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg
    38821 tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca
    38881 aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta
    38941 ccttggataa agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac
    39001 tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat
    39061 acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat
    39121 tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga
    39181 agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg
    39241 ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtaggatttg gtacaggtaa
    39301 agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt
    39361 tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg
    39421 tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg
    39481 tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac
    39541 accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga
    39601 agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata
    39661 atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc
    39721 gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat
    39781 gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa
    39841 tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt
    39901 aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg
    39961 taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt
    40021 gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt
    40081 cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga
    40141 agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa
    40201 agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc
    40261 aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat
    40321 ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta
    40381 tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta
    40441 aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta
    40501 agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact
    40561 gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg
    40621 aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta
    40681 ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt
    40741 ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt
    40801 acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta
    40861 tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat
    40921 gaagattact ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc
    40981 attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa
    41041 cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg
    41101 ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct
    41161 acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa
    41221 gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg
    41281 atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa
    41341 aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag
    41401 tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg
    41461 gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc
    41521 ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc
    41581 ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc
    41641 ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg
    41701 gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc
    41761 gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct
    41821 ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat
    41881 cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc
    41941 acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat
    42001 tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg
    42061 atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga
    42121 agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga
    42181 gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta
    42241 taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc
    42301 agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca
    42361 gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat
    42421 cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc
    42481 gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg
    42541 aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa
    42601 acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct
    42661 tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta
    42721 cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag
    42781 accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat
    42841 ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata
    42901 aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt
    42961 acttatatct atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta
    43021 tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa
    43081 gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt
    43141 ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga
    43201 caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag
    43261 aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt
    43321 aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat
    43381 aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt
    43441 tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa
    43501 actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga
    43561 cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact
    43621 gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt
    43681 gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt
    43741 cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat
    43801 tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac
    43861 tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac
    43921 taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga
    43981 cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga
    44041 aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt
    44101 tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc
    44161 tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa
    44221 taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg
    44281 tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt
    44341 gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc
    44401 gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc
    44461 tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga
    44521 agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt
    44581 acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa
    44641 atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga
    44701 ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc
    44761 tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat
    44821 tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt
    44881 aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt
    44941 tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt
    45001 caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac
    45061 agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac
    45121 taataacatc actttataga tgattctttt agagtgttat gatgttggaa gatatttttc
    45181 cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat
    45241 aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa
    45301 aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac
    45361 tttaggatga agaaggagta atcatggagc caattattga attagaaaag ataaattata
    45421 aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag
    45481 gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga
    45541 tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg
    45601 aagaaaatat ttggacgatc cgacagatgg ttggaatggt ttttcaaaat ccagacaatc
    45661 agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc
    45721 ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt
    45781 ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg
    45841 gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc
    45901 cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga
    45961 ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga
    46021 tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg
    46081 aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag
    46141 aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat
    46201 ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag
    46261 agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca
    46321 taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag
    46381 cggtacagtc catattggag aacgagatat tcagccggat acggataata agaatttaaa
    46441 gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga
    46501 aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc
    46561 actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg
    46621 ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc
    46681 catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag
    46741 aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt
    46801 agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa
    46861 gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa
    46921 agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg
    46981 gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta
    47041 tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt
    47101 ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa
    47161 tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt
    47221 ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct
    47281 ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt
    47341 ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct
    47401 gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg
    47461 aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc
    47521 acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa
    47581 cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag
    47641 ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc
    47701 gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa
    47761 cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat
    47821 tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc
    47881 ttttcttgct gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg
    47941 gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa
//
LOCUS       DASJTM010000011        47796 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJTM010000011 DASJTM010000000
VERSION     DASJTM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.011"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..1184)
                     /locus_tag="U4B29_000598"
     CDS             complement(33..1184)
                     /locus_tag="U4B29_000598"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN2105568.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1197..1634)
                     /locus_tag="U4B29_000599"
     CDS             complement(1197..1634)
                     /locus_tag="U4B29_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2105569.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1649..3415)
                     /locus_tag="U4B29_000600"
     CDS             complement(1649..3415)
                     /locus_tag="U4B29_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN2105570.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3679..4356
                     /locus_tag="U4B29_000601"
     CDS             3679..4356
                     /locus_tag="U4B29_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105571.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4359..5486
                     /locus_tag="U4B29_000602"
     CDS             4359..5486
                     /locus_tag="U4B29_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105572.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5686..6126)
                     /locus_tag="U4B29_000603"
     CDS             complement(5686..6126)
                     /locus_tag="U4B29_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2105573.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6137..8170)
                     /locus_tag="U4B29_000604"
     CDS             complement(6137..8170)
                     /locus_tag="U4B29_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2105574.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8172..9875)
                     /locus_tag="U4B29_000605"
     CDS             complement(8172..9875)
                     /locus_tag="U4B29_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2105575.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9969..10919)
                     /gene="manA"
                     /locus_tag="U4B29_000606"
     CDS             complement(9969..10919)
                     /gene="manA"
                     /locus_tag="U4B29_000606"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN2105576.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10958..11983)
                     /locus_tag="U4B29_000607"
     CDS             complement(10958..11983)
                     /locus_tag="U4B29_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2105577.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12192..13061)
                     /locus_tag="U4B29_000608"
     CDS             complement(12192..13061)
                     /locus_tag="U4B29_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN2105578.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13520..15412)
                     /locus_tag="U4B29_000609"
     CDS             complement(13520..15412)
                     /locus_tag="U4B29_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105579.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15409..17136)
                     /locus_tag="U4B29_000610"
     CDS             complement(15409..17136)
                     /locus_tag="U4B29_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105580.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17152..17802)
                     /locus_tag="U4B29_000611"
     CDS             complement(17152..17802)
                     /locus_tag="U4B29_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2105581.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18006..18302
                     /locus_tag="U4B29_000612"
     CDS             18006..18302
                     /locus_tag="U4B29_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2105582.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18474..19094
                     /locus_tag="U4B29_000613"
     CDS             18474..19094
                     /locus_tag="U4B29_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN2105583.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19186..20352)
                     /locus_tag="U4B29_000614"
     CDS             complement(19186..20352)
                     /locus_tag="U4B29_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2105584.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20364..20717)
                     /locus_tag="U4B29_000615"
     CDS             complement(20364..20717)
                     /locus_tag="U4B29_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2105585.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20845..21867
                     /locus_tag="U4B29_000616"
     CDS             20845..21867
                     /locus_tag="U4B29_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN2105586.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21886..22047
                     /locus_tag="U4B29_000617"
     CDS             21886..22047
                     /locus_tag="U4B29_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105587.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22306..22740)
                     /locus_tag="U4B29_000618"
     CDS             complement(22306..22740)
                     /locus_tag="U4B29_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105588.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22850..24709)
                     /locus_tag="U4B29_000619"
     CDS             complement(22850..24709)
                     /locus_tag="U4B29_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2105589.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24827..25987)
                     /locus_tag="U4B29_000620"
     CDS             complement(24827..25987)
                     /locus_tag="U4B29_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN2105590.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26294..27718)
                     /gene="araA"
                     /locus_tag="U4B29_000621"
     CDS             complement(26294..27718)
                     /gene="araA"
                     /locus_tag="U4B29_000621"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN2105591.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27734..28432)
                     /locus_tag="U4B29_000622"
     CDS             complement(27734..28432)
                     /locus_tag="U4B29_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2105592.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28389..30050)
                     /locus_tag="U4B29_000623"
     CDS             complement(28389..30050)
                     /locus_tag="U4B29_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN2105593.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30268..31668
                     /locus_tag="U4B29_000624"
     CDS             30268..31668
                     /locus_tag="U4B29_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN2105594.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31920..32999)
                     /locus_tag="U4B29_000625"
     CDS             complement(31920..32999)
                     /locus_tag="U4B29_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2105595.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33085..34044)
                     /locus_tag="U4B29_000626"
     CDS             complement(33085..34044)
                     /locus_tag="U4B29_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2105596.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34056..35567)
                     /locus_tag="U4B29_000627"
     CDS             complement(34056..35567)
                     /locus_tag="U4B29_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2105597.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35589..36152)
                     /locus_tag="U4B29_000628"
     CDS             complement(35589..36152)
                     /locus_tag="U4B29_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2105598.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36158..37021)
                     /locus_tag="U4B29_000629"
     CDS             complement(36158..37021)
                     /locus_tag="U4B29_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2105599.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37035..37931)
                     /locus_tag="U4B29_000630"
     CDS             complement(37035..37931)
                     /locus_tag="U4B29_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2105600.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38106..39395)
                     /locus_tag="U4B29_000631"
     CDS             complement(38106..39395)
                     /locus_tag="U4B29_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2105601.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39367..40884)
                     /locus_tag="U4B29_000632"
     CDS             complement(39367..40884)
                     /locus_tag="U4B29_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2105602.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41082..41972
                     /locus_tag="U4B29_000633"
     CDS             41082..41972
                     /locus_tag="U4B29_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN2105603.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42045..42869)
                     /gene="nadE"
                     /locus_tag="U4B29_000634"
     CDS             complement(42045..42869)
                     /gene="nadE"
                     /locus_tag="U4B29_000634"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN2105604.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42881..44353)
                     /locus_tag="U4B29_000635"
     CDS             complement(42881..44353)
                     /locus_tag="U4B29_000635"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN2105605.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44990..45352)
                     /locus_tag="U4B29_000636"
     CDS             complement(44990..45352)
                     /locus_tag="U4B29_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2105606.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45575..46327
                     /locus_tag="U4B29_000637"
     CDS             45575..46327
                     /locus_tag="U4B29_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2105607.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46467..47390)
                     /locus_tag="U4B29_000638"
     CDS             complement(46467..47390)
                     /locus_tag="U4B29_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2105608.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47405..47677)
                     /locus_tag="U4B29_000639"
     CDS             complement(47405..47677)
                     /locus_tag="U4B29_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105609.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13902 a         9941 c         8253 g        15700 t
ORIGIN      
        1 tttcgtcaca ttcccctttt tcgtatccta ttttaagcga cttgcttggc ataacggttc
       61 acgttttgct tgatattccc gattgttttc ggatcattga ttcctgtgac ttcttgaatc
      121 aattgttcca agttttcatg gatcttcatt tcatgcaatt gacggctttg ttcatcttca
      181 ggatcatagt aattaaaaat aatccccatt gttgccgtca aatgtgggca agtcaagtta
      241 tgttcttgta gttctctcac tggacgtgta aatctttctt gataacctaa cttacgtaat
      301 ggtgtccgag cgacacgtgt gatcgcatca gagatatttt tgttttggaa gcgatgaatg
      361 attttttcaa tatacgcatg atgctgctct gcatcaaacc cccatttggc aatcagcaaa
      421 cttcctgttt cctgcaaaac tgattttaat tgtgccacaa ctaacgcatc ttgcattgct
      481 tcatcaatgg tctggtaacc taacaaggca cctgtataag cgacagtcgc atgaccagta
      541 ttgacactaa atagtttgcg ttcgatatat ggttctagat catctacata taaaacccct
      601 tttaattgga tttttttatt ttttctttgg ctatcgtcaa tgacccattc tttgaaaggt
      661 tccacttgga cgaataaagg atcttcatgg tgttgtaatg ggacgattcg atcgactgct
      721 gcatttggaa agccgatata tttgtcagta aatgatgttt ccggaagata tttatacact
      781 tctttttcta gaaatgctga accgccaatc atattttcac aggcaatgat atctaaagat
      841 gttgtgcttc cttccttttc tctttcttgg ataccttttg caatcaactc agcaataaaa
      901 ggtaagatat tcgggccaat ggcggtagta actaaatctg cttcagcgat ttcttggatt
      961 actttttctg gatttgtttg attgttgatt cctttgacat tttttacagt gattttttct
     1021 tttgattcat ctgctaattc gatttcgtat tcttttcttt catttaaagc atcaatgatc
     1081 gtgtgattca catctacgaa agtaatctca aatccattag ctgccaaaac ctctccaata
     1141 aatcctcgac cgatatttcc tgcaccaaaa tgtactgctc tcatttttct ctctccttaa
     1201 gcaattgcta gattttccgt tacttcttct ttgctgagcg cgtctgctag ctggacaaca
     1261 ttgtccatat cactgcaata aagtgcgatt tgctgtacaa gttgcaaatg ctcatctccg
     1321 actcctgcaa taccgaacaa aacagtcgca atcttttctt cttcttcggt accaaaatct
     1381 acaccatctg gtacttgaac gacacagatg ccggattttt tcacatattt ttttgcttca
     1441 tctgttccat gaggaatcgc gataaaattc cccatataaa cagataacat tttgtctcta
     1501 tctaccatgg catcgatata ttcctcatca acgccccctg cttctacaag catctgaccg
     1561 caatagcgga tcgcttcttc tttggtttta aaagatcgat tgagctcgat catgtcgaat
     1621 ggcaaacttt gcatgctgtt ttcctccctt aagctgtctg tttcaaatat tgtgcaatcg
     1681 attcgtattt ttgcggattg accatgctgt tgactgataa atgaatggat tgaggtgcag
     1741 ccatctgtac tttttccgct aaactttctt tggctacgat cagtgaatca ggttcatctt
     1801 ttaaatcttg gatattgact tttcttaacg ggatagttaa atggttcttg tctaatatat
     1861 ctttgagcac ggtcatcccc attgttgctg atccggaacg atcatcgtgg acaagtagaa
     1921 tctctttgat ttgtttgatg tttgctgaaa gaaactcttc tgctggctca ttctcaattt
     1981 cagaaactga tgttttctgc ttgctgctta attcttgaat gatttcatca tatcgaggtg
     2041 aattcaaaaa gttttctact gagacaaaac gcgcgctcgg tgctttttgt tttgctcttt
     2101 cttgtagttc gacctgagta acgatcaatg tattcgagtc atctgtcagt tggctgattg
     2161 cttgattggt caccgtttga ggcaagtctg cttcctgtac tttttttctc aagatcgaag
     2221 cgcccatcgc acttgatccc attcctgcat cacaagcaaa aatgattttt tgtacgccag
     2281 agaatgtagc tccttgagga ataccttggg cacctttgga agcttgctta gctgcctttg
     2341 tctcttgaac agatttttcg aagtcttcat ttgtatcttt cgcatccgct ttcaaaatta
     2401 ctgcagaaat accaaacgaa acagctgcgg caacaatcac gcccaaaata acaggaaggt
     2461 aagaattgaa cggtgtcatc gctagaatcg caatgatcga ccctggagaa gctggcgctc
     2521 ttagtccagc atccatcaat tggaaaatga aacttcctga cattcctccg cctataactg
     2581 ataagaataa taacggtttc atcataacgt aaggaaaata gatttcatgg atcccgccta
     2641 aaaagtgaat gatcatggct cctggtgctg aagattttgc tgctcctttg ccaaacaaag
     2701 taaaagcaag taatacgcca agtcctggac caggatttgc ttcaagcaag aatagaatag
     2761 attttccagc ctctgctact tgttcagttc ccaatggtgt caaaatccca tgattgattg
     2821 cattattcaa aaataagatt tttgctggtt caataaaaat acttgtcaat ggaatcaaat
     2881 gggcagataa aatgatctct acaccttttg ccattgcttg agtcaatgca tcgacaactg
     2941 gaccaatcgc agaaaaacct aataacgcta atccaaaacc gataagacct gctgaaaaat
     3001 tattgactag catttcaaaa ccggatttga tccgtttttg gaatagctga tcaaatttct
     3061 tgatggcata tccaccaaga ggtcccatga tcatcgcgcc tagcatcatt ggaatatctg
     3121 ttccgacaat cacacccatc gttgcaattg ccccaacaac tgatccgcgg tcaccagcaa
     3181 ttacttttcc gccagtataa ccgatcaata aaggaatcag ataagttagc attgggctta
     3241 ccatggtagc caaagcctca tttggtaaat aaccatctgg gataaataga gccgttaata
     3301 caccccaggc aatcagtgct ccaatatttg gcatgaccat gcttgataaa gtactgccta
     3361 atttttgtac cctcgctttt agcgaaatgt tgtttttggg aatcgtccgt tccattttgt
     3421 ctctccctct ctccttttga tactctaatt gtatccgctt actaaaaaga gaaatagtcg
     3481 tactttggca cgaaactttg tgccaaaggt ttgagtatgt cagcaaaata aaaagatttc
     3541 ggtatcagaa aacttcaatt ttaaataaaa ctgttatttt aattttttta taatatgcaa
     3601 acaataactt ttgttagcat ttaatttaga tgttttccat tctattatgt tattaaattt
     3661 tatattggag gcctatctat gacattactg atcaaaaaca tgaacatgaa acgtggtaaa
     3721 aaacgtgtga ttcgacaatt gaatctttta gtagaatcag gagaagtagc tgctttgatt
     3781 gccccgaacg gatttggcaa aacaactttg ctgaatggca ttgcccaact gctgccaaca
     3841 acttatgatc aattgtccct tcacaacatc tctcctactg cttatacaga ttatcgccgg
     3901 tctttctttt ttctagaatc aacgaatcag ttatccgact atctatcggc tgttgattat
     3961 ttgactttga tcaaatacta ttggcagagc aaagtagaat gccaaactgt tttccaacga
     4021 ttgaagatga ctgagtacct tactttgccg attggaaaaa tgtctctagg aatgaaacaa
     4081 catgtcttgc ttgctgctta cttgatcagt gattcatctg tcatgttatt cgatgaaccg
     4141 ttgaacggat tagacccagg aagtattgat atcttgaata gcctcatcag acaatggcag
     4201 aaagagggga agacgattct tctttcttcc cacgctatcg cgaatatcca atcgatttgt
     4261 tctaaagcat gttttctaaa aaatggagaa atcgtcacag aatcctcaaa tatggaagag
     4321 attttagcta tttatcgtaa attatatgag gtgtaactat gtctttactt acatttgaac
     4381 gcaatctctt actaaaaaac aaagtaaact ttttatttcc tctattgttg gtcgtgctct
     4441 ttgcattccc actttttttt gatcataaac ttgcttatac agaattcgat gaactgaatc
     4501 ataactatga agagatgcaa cgtttgattg aaacgttgaa agaagatgag aatgagaaag
     4561 aatttgtgga aagtttagag aaaagcaaca aactgatcga agcaatctta catgcaaaaa
     4621 acacagggaa tgtccagcaa atggtcgaag caacttatca ttatgaaaaa gatatactgg
     4681 atcgactgat ttctggtcaa agacaaggaa ttccaatcat tgaacaacaa aaacgtgtcg
     4741 agttgctccg ttatatgaaa gaacatcaga tccaaagata ttctattttt gacttgccag
     4801 cgcatctccc tttagcaaat tactacgaaa atattttttc aggaatgatt tctagttttt
     4861 tgattttatg tataaccgcc ttgtttcttt cttccatcat ctcttatgaa aaaagaaagc
     4921 aggtaatctc tctagtcaat ctattaccgg atagtatggt aaagaaacat agcatccgtt
     4981 ttaccattta ttatggtgca gctatgctaa gtctcgtgat gccatttttg atcgtctcta
     5041 tactagtaat aatcaaaaat ggtttgggag acttccgtta tcctgtagga acgatcattg
     5101 gtcaggagat acgaatcctt ccgatgtatg agtatctttt ccagagcttt ctgttcctgt
     5161 tgttgtgggt gctcttttta agtacaatca gttttcttct atctgcttta tttgaacatt
     5221 ctctagtcaa tcttttaggt acacttcttt gtcttttctt agcagagtat cgcttgtttt
     5281 cttcgattgg atggatagaa tcaatcagtc attacttgcc aacttcttat gttgattttc
     5341 aaaatgtgat cataggagga gatattttct cgccactagc ttcagaacag gttactttca
     5401 tgaatggtat cttgacactt ggtatatgga gtatcgtttt attattcata ggtatgggca
     5461 ctatctatat aaaaaagagc tattaaaaca caaaaataat aggggcggtg acaaaaacct
     5521 tgtcaccgtc ccctatttca aataaacggt tttctagaat aaatagtttt caaaagcttg
     5581 catccgcagt aataagaacc tcccaacaaa aatagctcct cttattttta tcttggttga
     5641 agttattact tgatgcaagg cagctttttt tactgtgaac attggttata tatgtccatg
     5701 ttcaaaaatc atttggactt cttcaatggt tttcgcatca ctgagcgcca ttacttggtc
     5761 gatgtctgaa cagaaaaagg ctaaatcttg gagcaccgct aactgatgtt ctttttttaa
     5821 agccactaca aataagagtg tcgcgatttt tggttcctct tttgttccaa agtccacacc
     5881 atcaggcact tggactaaat agattccttc ttctactact tcttcatctt tgtcagtccc
     5941 atgtggcaag gctacgaaat tcccgatata gacactcgct ttttcttgtc tttcgatcat
     6001 tgattggata tatgccggtt tgattccccc ttgttgggtc atccactggc cgatctcggt
     6061 caacgcttct ttccaatttt gataggattg atcgagcaat attttttctg ctggtagctg
     6121 tatcatcttt tcaccttcat ttctttattt cttcaatcag gattttcgct aacaactgat
     6181 aaacgatttc tttatttcct gagcggaaga tctcggtgtt caagtcattc atgatgattg
     6241 ctccactgat ctttcctaaa agaatcccgt ctttttcttg gattggcgaa ggcgccagca
     6301 taactaacat acgggacaaa tcgactggct gtctatccat cccttcaatg gtcaacggat
     6361 gtttcaaatt gaaaatacaa aagatcggtt tagaaatcaa tgagctggat gtatggaata
     6421 accccatatt tgtatgagga ataccgatag gtgcttgttc atatcgctta agcagccggc
     6481 gatgaaccac ctcttcatcc gttactagtc cttcaggaaa ataaggaaat aagctgcgaa
     6541 tcgtttcgga aatcgtctca ggattatcca gttcttggat aaaaaaacga ccgagtaatt
     6601 gattgatttg atccatgaaa tgcattgttt cctcataagt atcttctatc atccactcgt
     6661 tattgctgtt atacgtaatt ttttgccttt ttttcttctc aatatgcgaa aaagcttctt
     6721 ttagctgctt gacttcatct tctagcagta gcggggagac taaaatatat tttccaccgt
     6781 atcctggtaa aatagatgta gaaataacaa catcatattc ttcttgtaaa tcgatttttt
     6841 ttaaatcagc aatccgatag aaatgaattt gttggatgaa gggaaaatac cttttcaatc
     6901 gtagttccaa catgctagta gaagctaatc cacttggcga aaatcctgca atactgatat
     6961 tgctttcttt cggacttcgt tccaatgaat tcgcaaaatg aaggaccata taagcgatct
     7021 cttcttcgga taattttttt tcttgaaaaa tccgctctac tgctttacgg attgcattcg
     7081 caacttcctc gtattgaacc atgatttttt ctaaaatcgg attggtcagt gtctcttctt
     7141 gtaaaacggc tcttgaaaac acaccagata aatgagtcaa cagcattttg tacaaagttt
     7201 catcttcata aaaatggaca ttcgtctctt cacttacttc ttgaatcagt tgttttacta
     7261 aataagcaag ctgtgtatcg aacgtctctt caaagaaatt ctgatcaaat gagttcgtga
     7321 agtcatttaa taatcctgca aaaaagacga tctcatttac tggataaagt tgttttgtct
     7381 cagaagcgat ccgtgaaaac aatcgtttag ctgtctgtag taattctttg tttgccagac
     7441 tattatatgt ctcatttgtc agtaaatgat ttttagtcac acgctccatc gtcaatgtca
     7501 gcatcaaaac caaatgttca agctttctat cacttagatt ggtctttttt tggatcaatt
     7561 cttccccgat tactttgcgt gcaagagata gatgttgcgg atcaacgaat cgaaaaaaat
     7621 aattcagtga tgcatcgaaa gaaatactat gaaaaagttc atattcattg atttctaatt
     7681 ctaatacatt cgctgtcaga agccggcggt atttttccgg tccttcgatc tcgtatccct
     7741 ggttccgaat gatttccaaa ggcagttggc gaatactttc ttccaactgt ttgatatctg
     7801 cataaaaagt ggtattactg atgcgatatg attccatgaa atgagcaaga ctcaacggct
     7861 ctgtcgtcag taacaactcg gctaaaatcg cgtgttgtcg tttagcagtg gataagtcat
     7921 tacttttccc atctgtattt tcttttaatt gctctaatac ttcttctgat gcctcaatga
     7981 caaatgagcc tctagctgct tttttcaaag aagcatttac cgatttcaat gagacttcaa
     8041 gattatctaa ttcgcggtaa atagtccgtt tactgacctt caataaattc atcatttttt
     8101 ggatagatac aggtgacggg ctactgagaa attctgctaa taattgtttt tctctgggcg
     8161 ataaatacat cttatatttc tcccttcacg agtttttcgt attcattcgt agctacccaa
     8221 tgttcaacta gaactagccg agggatcttt tcttctagct gttcagttac agctagttct
     8281 ctttgtgcga tataaatcgt ttcttcttgg attttgattt tttctattga ttcttttgag
     8341 ataacaaact gttttttcgc tttttttgct aattgactta aaatcgtcat gcccatagtt
     8401 tgtgagccgc gtctgttgtt tttatacaaa aatacaatat tagcaggcga ctttttttgt
     8461 tctgtgatat ctgctatctc ttttttttct tcccgctttg gaaattggat cgcttctttt
     8521 attcgtgaca gcaagatatc tgtttccaaa aagtgatcca gtacatagat ttgactttga
     8581 ggtacttttt ctcttgcaag acccgctagt tcatgttgca cgataaccag ctgatttact
     8641 tggttattca attcatagat agagctgtat ccgacagaaa gatcgatttt ttctgctgct
     8701 aatttctttt tcagcagggc ggctcccata gcacttgatc ccatacctga gtcgcaagca
     8761 aaaatgacgg aatgaatgat agatggttcc aagatttctc ccctttcttt gaccgttttg
     8821 ttctcttttt ctttatttgg taatttcaat agtatcgctg cacaaatgaa ggtcacaaat
     8881 gtactgattg ctatccctat acttacccca gctagttgtg gataaggcgc attggcaaaa
     8941 atcacgagta gtgacccagg cgatacaggt gcagctaatc ccgtatctaa ccattgaaat
     9001 atcaacgtgc cgcttgcccc accagccatt acagctaaaa atagatacgg tcgcagcaag
     9061 acaaatggaa aatagatttc gtgaatcccg ccaatcgcat ggatcatcaa tgctccactc
     9121 gcatttactt tttcctgttt ttctgcaaaa caaaggatag aaagtaagat ccctaaacca
     9181 ggtccaggat ttgcctccat caaaaataag gaagagtacc cgactgtttg tgagaattcg
     9241 attcctaaag gtgtcaatac accatgattg attgcattat tgaaaaatag tacttttaga
     9301 ggttctaaga tcagactgac aagtggtaac agattcattg tgactagcca agacacaacg
     9361 gaggcgagca acaagtgaac actatttaaa actggttcaa taaaaacgat tccaattata
     9421 caaataacgc taccagtcag tcctactagt aggttgctga caagcatttc atatccagcc
     9481 ttgaatttca cgtggaatct agaaacaaat tgttcatata cgatgccggc aagcggcccg
     9541 ataatcatcg ctcctacgat ttgtggagca ttggaacgag cgatcaaccc taaagaagca
     9601 attgcaccgg cgactgcccc tttttgtccc cctaccgatt tcccgcctga ataactgatc
     9661 aagatgggta aaagcagctg gatcatccaa tggcatacct cttctaatgt ggtgtgcaaa
     9721 tcgccagaca caccttttgt tgctaatgac aagattcccc aagcgataaa cagacttaga
     9781 tttggcatga tcatttcatt taagtgatgt ccagcttttc tcagttgttt catagacacg
     9841 cgactgctgt tgtttgcttt ccatttgtat tccatctttt tcaccatact tctatttatt
     9901 gatggacgga ttttatgtag aaaaagcagc aaacgaaaaa ccgtctgctg ctttcagcta
     9961 tctgggaact atttgcctgg ttcagtcgca atgattgcta gttcaccttg gaccgcccac
    10021 tcagaaacgc catttggaat aatacagctt gttccttttt ctaatggata tgtttttccg
    10081 tcaacgacaa ggtctcctgc tccttcgatt accgtcatca atgtataagg ggcttgtttt
    10141 ttgaaagatg tgattccttt aatatcccac tcatatacat tgaaaaactc agtttctaaa
    10201 taagtgacga tcgatgaatt cccttttcgg acttctttga tttgtaattc tggtgttttc
    10261 gcagggacag ttgttacatc gatcgattgt tggatatgca gctctcttgt ttgtccttgc
    10321 gcatcttttc tgtcgtagtc atacacgcgg taagtcgtgt cagaactttg ctgtgtttct
    10381 aaaatcatga ttcctttacc aatagcatgg atcgttccgc ttggtacata aaagaaatca
    10441 ccttttttaa ctggtacttt cttcaaaagg tcatcccagc gcccatcttt gatcatctca
    10501 gctagttctt ctcttgtctt tgcatgatga ccatagatga tttctgcacc aggttcagca
    10561 tcgatgatat accagcattc agtttttcct aattcacctt catgtttcat cccataggca
    10621 tcatctggat gtacttgtac agatagatca tcttctgcgt ctaagatctt gatcaataat
    10681 ggaaacactg gttctgaagg atggccaaac aattccttgt gttctgacca tagttcatct
    10741 aatcttttcc ctttgaattc gccattttct actacactga caccatgagg atgcgcactg
    10801 atcgcccaat cttctccgat tttatcgtta gggatatcaa aaccaaagac tgaacgtaaa
    10861 cgacttcctc cccaaatttt ttcttgaaaa actggtttta aaaacatagg ttcctgcata
    10921 attcaaactc ctctcatttt tcatagctat tatagcatta tttcggtaat accgttttct
    10981 ctttcaaaaa gttttcaaca atttcatctc gacatatgag atactccgca cgatgatcga
    11041 caggatgtac gcgatttgac aaaaatatca acgcttcttg tgtttttata tcaatcaaga
    11101 aaaacgtacc tgtataacca gtatgaaaaa gaatcgcatg accgtcttct acatcctttt
    11161 ttaagtccca tcctaaagaa cgatagccat tttttttcgg tgtttgatct tgcagtaaag
    11221 aatcaatcgt ctcttcttct aaaaaacgac cattacggat ttttcccttg ttcaaataca
    11281 tttggacaaa agtaatgctg tctttcaaat caataaagag acccgcgtta cctgcatgtt
    11341 cagccaaaac tcttgctttc ggatcatgag tttgtccacg caaaacctca cccgaaggca
    11401 gttgttctgt gggaacgatt tttttggaag gaaaaggaag aaataagctg ttcatcattc
    11461 ctaatggttc caatacttgt tcttgaaaga tttccactgc agtttcttgg taaatttcct
    11521 ctagcatcaa cccaagaaga atcgtaccgg catctgtata ttttacttct tttcctagtt
    11581 gctcacctgg ttctagcttc aaataagcgg cgatcaattc ttcctgagat agcttatctc
    11641 tgtttggtat ccatgttttg atatcagaag tatgtgtcag taaatgacgc aaagtgattg
    11701 tctgattttc aaattcagga agatatttat gaagcggttg atcgagttct atctttccat
    11761 cttctaatag acgtaaaact aatgaagtgg ttgcaacgac tttcgtcagt gaggctgcat
    11821 caaacaacag atcttctgtc atcagctgct tttcaggaac gacttgggca aagccggctg
    11881 taaatgtttc tacttgattt tcttttatga aggcgactgc tacacctgga taaaatcctt
    11941 cttccatctt tttcaaaata agctgtttcg tgtgtggata catctttttc tcctgctttt
    12001 ctatcagatt cttacagtcc aagcttaaag cttaatctat gaggaatcaa ccttttttac
    12061 acaaaaaaac tgcgcgaaaa accgcgcagt ttcccccggt atactatttt ctctttctat
    12121 ctctagaata agcgatagta tgaccgttca taaaatgacg agacatgcga caatcgttaa
    12181 gaaagaaaaa gttaagcgtc gcatcgaatg tcagttttat tcttttcttt tggctcactg
    12241 acgaaccacc attcgattcc tatagcatca tagattgtga gtactgtgtg ctttttgtct
    12301 aagaagaaat caactgatag ttgctgcaag tgagcaatca gtccttccat gctttcagta
    12361 tcgatcatga atttcagaaa atcaagtcca atcacttcat ctgtaggcaa atcaattgcg
    12421 cctccgctag cttctgtcaa accaacatgg aaatctcctt cattcaatac atggatgccc
    12481 tcttcttcat tatgtacctt caaaccaagt acatgattaa gaaattggcg ttcttttgtt
    12541 aagttagtaa catttaaatg gattttttca aaataagaac ctgttgatag tttactgaat
    12601 ttttcttcag attttcttaa taatgcttct tgatccattg tttctggaat ttctctttgg
    12661 tcgttcttat tctcttcatg gtaaatttcc aattcattct cttctgggtc tacgagcaaa
    12721 atccccatac gtccatgatc ttctaaagct tctttgattg gataattcat tttacggata
    12781 cgacaaagga tatctgccat ttcttctgtg ctagggataa tcaatgaaaa acggttcagc
    12841 tttttgatca cccccatgtg atcattcgct ctaggacttt cttccagcag caacatttca
    12901 cttccagctt cttctgttcc taaaatggct aattcgtttt cttctctttt caaatcaaag
    12961 ccaacaacgt tcttgtaaaa atcaatcatt ttatctcggt ctttcacccg aatggctaca
    13021 ctttttaaat aggtggcagc ccccaattga aaatctttca tatttcttgc tcccctctac
    13081 gtcaagcttt attttaacga tggattcttc ttctttcaag caataacttt gattttgtta
    13141 aaaaaaatgt gagaaaattc gcattttgct tgttttttgt aaattaatga gtaaaaactc
    13201 ttataaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa
    13261 aatagctcct cctatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    13321 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac
    13381 ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
    13441 aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa
    13501 ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat
    13561 ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac
    13621 catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa
    13681 cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc
    13741 gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg
    13801 cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta
    13861 atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag
    13921 cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga
    13981 tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat
    14041 cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt
    14101 agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag
    14161 cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg
    14221 tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa
    14281 ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg
    14341 aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga
    14401 actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa
    14461 cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca
    14521 acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat
    14581 tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat
    14641 gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg
    14701 cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca
    14761 tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa
    14821 tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt
    14881 tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg
    14941 aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt
    15001 gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca
    15061 tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta
    15121 cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg
    15181 ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta
    15241 caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg
    15301 ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat
    15361 gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc
    15421 ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc
    15481 ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc
    15541 cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt
    15601 caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa
    15661 cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc
    15721 accagagaag tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga
    15781 aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc
    15841 cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg
    15901 aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt
    15961 tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt
    16021 tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc
    16081 aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc
    16141 caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc
    16201 gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat
    16261 gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa
    16321 tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt
    16381 gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat
    16441 cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc
    16501 ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa
    16561 taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta
    16621 catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa
    16681 ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat
    16741 catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct
    16801 tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt
    16861 tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa
    16921 tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt
    16981 atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag
    17041 tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc
    17101 tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata
    17161 tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga
    17221 gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt
    17281 catcactaat tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg
    17341 atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg
    17401 cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat
    17461 aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga
    17521 aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat
    17581 atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc
    17641 ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat
    17701 cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt
    17761 ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggatcc tcctttcttt
    17821 caagatgaca cattgtcata tgacgctgtg tcattataac acctcctacc tgttttgcaa
    17881 gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc
    17941 cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga
    18001 gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca
    18061 gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta agccactcgc
    18121 tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt
    18181 attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta
    18241 atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt
    18301 gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt
    18361 ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt
    18421 ttctttttct catagttata acaatcatag caataaaagg agaaggtaag catatggcat
    18481 ctgttttagt agtcaaaggt catcccctaa ctgcagaaga atcgcgcaca gttaaggcat
    18541 tgacgtcttt tttaacaagt tataaagaaa atcatccaga tgatgaagtg actgttttgg
    18601 aactttatcg cgatgatatt ccggaaatcg atgaagaatt attatcaggt tgggaagcat
    18661 tacgtgctgg tgcagaattc acttccttat cagaaagtca acaacaaaaa attgctcgtt
    18721 tcaatgaatt gactgatcag ttccttgctg ctgataaaat cgttatcgca aacgccttat
    18781 ggaatctaaa tatcccaaca aaattaaaag cttggttcga taccgtcaat gtagctggaa
    18841 aaacgttccg ctatacagaa aacgggccag aaggattagt gacaggtaaa aaagcactgc
    18901 acatccaatc taatggcggt gtatacaacg gacaagactt tgcctctcaa tatgtaaaag
    18961 gtattttaaa ctttgtaggc atcgatcaag tagatcagct attcatcgaa ggaatcgatt
    19021 atgaccctga ccgtgcagat gagttgatgc aaaatgcatt agataaagct gctgctttag
    19081 gtaaatcatt ttaaaaattg agataaatgc atacaaaaga gacagaggaa aaaatcttca
    19141 aagcagcatt tgtaccttgg atattttccc atctgtctct ttttgttatt ctttctgctt
    19201 tttcaacgga ttttcttcat aaagagaaga gattgttgtg taccattcaa aaacatgagt
    19261 aatcactact ttagctgctg cataaacagg aatccccaag atcacaccga tgattccgaa
    19321 caatttccca gaagtcagca gaactaataa aatcgttact ggatggattg ctaactgatt
    19381 ccctagtact aatggagaga tcaagcgtcc ttctattgtc tgctctactg caaaaacgat
    19441 cagtactttg atgatcatga atggacctgc tacaatggct aaaaagatag ccggaatcat
    19501 cgctaaaaat gatcctaagt aagggatcag gttcaaaaaa cccgcaataa tccccaacgt
    19561 gacagcatac tctaaaccaa tgaccgcaaa accaatcata aacatgattg ccacagcaaa
    19621 agccacagtt agttgtcctc ttatataaga agaaacttga ctattcattt cactcagcac
    19681 attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg acaaaataag gtgctaattg
    19741 tttaccgtct ttcaatagat aaaataagat aaacggcatc gtcagcagag ccactaaaat
    19801 agtcgctacc gcgccgacaa aacttccaag actctgaact gtactcttag atatatcttg
    19861 gatcatatcg cctgccgaat tcatgaactt atcacctgct gtttccaact gttcgcgaaa
    19921 ttggttgaac agcgggtccc gcaaaatctc ggttaattta ttatcaatcg tatctacata
    19981 ttgagggaag ttactgatga agctcactgt ttgttcctga atcttaggga tgatcactac
    20041 gctcccccat gcgattagtg caacaaccaa aacaaacagc ccaatgatac tgtaaagcct
    20101 tggtactttt cgcttttcaa agtaatcaac taccggattc atcaagtaat acaagattcc
    20161 tgctaaaatg ataggcagac ctacgattgc taaaaattgc cagattggtg caaacaaata
    20221 tgaaactttt gtaaataaaa agacgatcag caatattaac aatacaacaa gtaaggctgt
    20281 gactacttga ttatttaaaa accagcgcca aaaccacgat accggtctgt tcttcttatt
    20341 ttttccttcc ataattcctc ctgttagtta taaaaaattt ggcttcccaa accaatagca
    20401 ggcagtctat gcggatgaag atagattcca tttgcaattt gtccttcttc tggacaagca
    20461 tcaaaaatca atgatacgtg agctactgtc tctaaattcc cattggccat cggaccgaca
    20521 taatcgatcg tatatacctg atcatcaaaa gcaattgttc cgcctgcttt caaagaaaaa
    20581 ggttcgtctt ttgatacttc ttgaatcacc gaatattttc gtaaggcagc tgttgctgtt
    20641 ttatcgaaca aaatcagcat gggttcttcc ttgtttattg cttctgtccc aatttctgta
    20701 atctttgcct gaatcattgc actctctcct ttaattgctt tcaaaaacag tataacacat
    20761 tctcattctt tagccgtgtg gaataattat gtgatagaat aaaaacgaaa actatttcat
    20821 tacaattagg aggaatcagc tcatatgatc gaaaagataa ttttaggaac ctatacgcgt
    20881 cgcgaaagta aaggaatcta tacgatcgac cttgatacag aaaaagaaga attagccaac
    20941 ctttcattag ctgttgaaga aaatagccct acctatgtag caaaaagtaa agctggcaat
    21001 ctttacacag ttactagtgt ggatggatta ggtggtgccg gtgcgtacga taaggatttt
    21061 cattttttga atgcggtaac agagtctggc gctcctttgt gttatgtagc ggttgatgaa
    21121 gcacgtcaat tactatacgg ggcgaactat cataaaggtg aagtaaatgt ctaccatata
    21181 ttaggagatg gcgaactgaa agcagcagat tcaatattcc atcaagaagc gacaggtcct
    21241 cataaaaacc aagatcatgc gcatgtccac tatacggatc tgactcctga ccaacgatta
    21301 gtcgtatgtg atcttggtac agatcgtgtg tatacttatg atgtgtccga aaacggcaaa
    21361 ttgaatcttg taactacctt cactgcagag ccaggaacag gccctcgcca tcttgtcttt
    21421 catccaaatg gacaattcgc ttatttattt ggtgaattag acagcacagt ccgcgtgctt
    21481 tcttataata aagaaacagg tgcttttgaa gaattacaga aaatatcgac cttgccagaa
    21541 gaattcgacg gtgaaaatgg aggggcagct atccgcattt caaatgatgg caagttcctt
    21601 tatgcttcta accgaggaca taactcgatt gctgtattcg cagtttttga agatggttcc
    21661 cacattcaaa acaagcaaat catctcaaca gaaggggatt ttccccgcga tttcgcacta
    21721 gatccaagca ataattttgt cgtgtgtgcc aatcagaaca ccgataatct gacattatat
    21781 cgccgtcatc ctgaaacagg attactggaa ttactgaaaa aagatgtcta tgtaccagaa
    21841 tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga atgaaatgaa tcagcaagac
    21901 gtattagaca gactacggga agaactaaat attcctttct ttgatgggaa aatcgaagat
    21961 aaagagtata gcgaagagga atatcagaaa ctcaaagccg atttacagaa ctactttgaa
    22021 caatatgtac agaatgtaga aaactaatca gctttccata ctatctgaaa agaacatcag
    22081 gacaagactt ttccgtctat cctgatgttc ttttctacaa ataaaaaacc gccgagatgg
    22141 ttagactcga cggaaaagga gtatttactc gattttgagt aagtacaagg ttgttgttgg
    22201 ttataggtat gtcattatca taaaggattt tgatcactct atcataaagg taatattaca
    22261 tttatattag ataactctag tttttattgt tatcaaagat ttcttttatt ttttcaacaa
    22321 caaattagtc attttttgga tggcagctgt caattggtct gtcgcttggt cttgttttgc
    22381 ttcagatgta ttgaatagat cttcatctac ccctgattca agatcgatac tttggatcat
    22441 ttcttcacag cctgctacat atgaaagata agctttttca aaatttttat gaatgcccat
    22501 tgcttttgct ggtggacgca aagtactgac tttcttaagc attgcccgat atttttttgt
    22561 tccttcttca aataacagga tgatctcttc aagagtcgat ttgtccagtt cacttgtttt
    22621 tccttcatca attgcgtttc tgatcacttc atatttcgga tgcattcctt ctccgattgc
    22681 ttctgtttcc ttgacgattt gatcgatgga ctgcacataa tagccaatat tcggtaacat
    22741 gttcgttttc ctactttctc cattgttgtt attatttatt ctacttgttt ttatcctcaa
    22801 atgacaagta cttgaaaaaa tttaatggtt tctatacagc tcgccattct tattcgtgct
    22861 caaagaaacc atctgctaaa ctatattgtt tccctgcgac aatgtcatat tggatcaatt
    22921 gatattcgtc gtaaatctct tgttgcgagc gattgaattg cgcttcttta taccactgtc
    22981 cgttttgata gtacagttct cgttcaaacg ccggaagttc ctgttctaat gataaaagca
    23041 gctgataaaa gccatttgtt gtctgattcg tctgttccat tagattcgct gcaaaataaa
    23101 acggactggt gattgcatcg tgagtcgttt gcttttctaa ttctccttta ctgtcgaaca
    23161 taagaaattg tgtttcatgc aatgtttgtt tgtcattttt tgcttggatc gtatctgaat
    23221 agatccctgg aagatgatcc ccccaaaaaa caactaaagt ccttctagaa agattcttca
    23281 attcttccgt gaactgcttc agtgccgtgc tgctatagga aatatcttgc agataattct
    23341 ctaagtcaag tgtcccactt ccatctgaag tttttgcagg atagtctaga ttatcatact
    23401 ttccatcgta gggcatatgt gtctgcatcg tgactaaatg aatgaactga ggtgtttttt
    23461 cttccttgag aagtgtcatt acttctttgt aagctgactc atcagaaata tatggattat
    23521 tctctatcgt atccgtatag gtcatcgtac gctcgctgat gaactggtca aatcctaatg
    23581 tctgataaac atcctctctt ttatacatcg aagtattgta tggatggatc gctgtcgtct
    23641 ggtatgattg tgcgtttaac gctgaaacga tagaaggaag ctgatccatt tttggtacaa
    23701 gcatcgtgta aggggtggtg agttgcgcat taaataacgc catagaaaaa ccagtcaatg
    23761 cttcaaattc gatatttgct gttccaccgc catagttttg agaaagcata ttgccactat
    23821 atgtttgatc agctacttca taataatctg ccaatggctc accagatact tctacgccgt
    23881 ttaatcgact aggatctgag aaactttcac tcatcacgaa cacgatgttc ggcgactctt
    23941 cctctacagt tttatttttt tcatcggcta gcttctggta tttttcagtg atttctttga
    24001 tcttttcttt cgaatatcct tcaggttcat ccattggttc taccttcaga ttgtacaaaa
    24061 acccaccgat aaaaccagtg ttatagtagt tcattttctg gctgtaaggt atccatagtg
    24121 ccgttttatt atatgctttt cgcaacaaat tatcgggatt gttgaaatta ctgatatacc
    24181 ctagcagtcc aattgccaca agcagtgtcg ttaatcggat caattggaat cgccttcctt
    24241 ttttcaggct tcgaaacatg taccagcaaa acaatcctat cacggttcct gcggcagcta
    24301 agatcactgt aaacagcata gtgccggtca tatctttcag caatccgatt tcagtgatca
    24361 tcttcaaatc gtctggataa atcggttctt gccgataact cattttcata taatttgcaa
    24421 aacctaatac accgatggct acgacatacg taagggaacc taaagggaca gatcctgcta
    24481 gtgcaatcag aaaaataaga ataatcagca agacgagaca ggctaagaag aatttttccg
    24541 tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga tagatcattt tgacaccact
    24601 gcaaaaataa attactaaaa acaacaagaa aaaagatact gatgattgct gctgttcctt
    24661 ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata tcttttcaaa tgcttgctcc
    24721 ttcatttcta tcaataaaag aatgttgctt ctcttatttt agttgtacga aattcgaaag
    24781 taaagaacgg aaagaagaat taatgaaatc caaatattct ctttctttat ggcctttctg
    24841 gtttctttaa taagcggacc tttcgtactc cagaaatttt ttctagctgt tgtttcactt
    24901 gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa tatgtagaca tgctcattct
    24961 tatggttacg cgatatatct gcgatcgcta gctcgttctc attcagcaga gaaaatatct
    25021 ctgctaatat attggttttc ttttgataaa atatagtgaa tcgaaacggc gcttggaaaa
    25081 acattcgtgc ggatggataa ttgaccgctt ctctaaccgt accgaacaaa agataatctt
    25141 ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc ttcctgagtc gatcccccta
    25201 gatgaggcaa cataagtata cggtcatttt ctaagaactc ttcttctgga aaatcagtca
    25261 agtatttagc cagatgttgt ttttccaatg cttgcataac agcatattta tcaacaattt
    25321 cacttcttcc tacattgata agtacagcat cttttttcat tttttctata ttcgcctgat
    25381 cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac tacgatgtaa tccgaggtag
    25441 aaagaactgt ttctaaatct gcttgccgaa cataatccaa ctgggcatcg cggatagaat
    25501 aacccaagac atccatccct aagctatagc aacttagcgc tacttttgtg cctatcgctc
    25561 caagaccaag aagaccgatg gttttgcctt gcaattctcg tcccacataa gcacttcttt
    25621 tgttttctgc ctgttctaaa atattaggac ctgttagtgt ttgtaccata cgacttgctt
    25681 cgattatcgg acgggaagat aagagcaaac agcacaaaac aagctctttt actgcattag
    25741 catttacacc tggtgtattc atgacgatcg tcccgttttc agaagctttt tcaacgttga
    25801 tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa atctggtgta agccattcat
    25861 ctttcacttt cgtactacga ataactattc cttcggctgg caaatcatct attattttga
    25921 aatattcccc ttgtaacttt tctatctctt gttctggaat agcatcaatc aaatgaacgt
    25981 gatacataaa accccctcct tactaacaag tatctcgaaa tttctgcaat tagacaactg
    26041 tttaccattt gacttcctag aagcaaaaag agcagttaga gcagagtagg ctgagtgata
    26101 aaaataaggc tatgacaagt tactgccata agccttattt tcgagagcaa aatattgagg
    26161 gtgagatatc ttgataaatg gtgttccgtt agctgacatt gataataaga ctaaatttca
    26221 aaaaagttga aaagcaaatt tcgtcaatct cttcttattt cttaaaacta agtggctttt
    26281 tcacaacctc tttttatcta ataaccactg tttccaactc aactaatttt gcccacgttt
    26341 caatttgatc tgttgttaga tttagcgaga caacggtatg atgaccgcca cctgcctgaa
    26401 tccatgaatg gacaccttca tggaaattcg gtttagtttt ccataacaca cgtgcaacag
    26461 gtaaattagg tgcttcagtc gttggttcaa aggcttctac ttcattgatc agcagtttat
    26521 aatgcgtacc gaaatctgcc atcgatacga ctaccccttc tcctgctttc ccatcaaata
    26581 caagacgcgc cggatcttct ctatctccca ttgataaagg agaaacaacg attcttggtt
    26641 tcgtcgcagc aagcgtcgga tctacttcca tcatatggga ttccaagatt gcttcttggc
    26701 cactagctag ctcgtaagta taatcttcca tgaatcctgt atctttccct cgagccatga
    26761 ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc ttccccagca aaaccgtatc
    26821 cttctgccat caaacgttgt acagctagtc caggtagttg tttcattcca tacaggtctt
    26881 caaaattagt cgtaaaagcg ttatagcctc cttcttctaa gaaacggcga ataccgattt
    26941 cctgctgagc ttgtactttt acatgttcct cccatgtctt ttcttcataa tcaccatagt
    27001 cgaaggtata caattccttg tactcttcaa ataattgatc gacttcttcg tctgatacgc
    27061 gatcgattac ttgtacaaga tctccgatac caaaataatc aacagtccaa ccaaattgaa
    27121 tctgtgcttc cactttgtct ccttctgtga cacccacgtt tctcatgttg tcaccaaaac
    27181 gagccacttt tattccaaaa ctttcattat aggctactgc aacatccatc caatcagcaa
    27241 tctctttttg gatctcaggt cgtttccagt agccaacgac gattttattt tgtttattta
    27301 atcgagcatt gatgaaccca tattcgcggt caccatgagc ggattgattt aaattcatga
    27361 aatccatatc gatcgttttc caaggaatgc tttcgttata ttgcgtagct aaatgaagca
    27421 atggtttttg tagaagcttt gtcccgcgga tccacatttt tgctggtgag aaagtatgca
    27481 tccaagtgat cacacctgct acttcttctc gataattgac ctctttcatg atttttgtga
    27541 tcgtatcagc agtaactgct aattcttgaa ggcggattgg ataaggcaat tggccatttt
    27601 cattcagttc atcgaccatt tcttgtgcat gtttctttac ttctcgcagt gcttcttccc
    27661 catataaatg ctgtgatcct acaacaaacc aaaattcttt ttctccaata ttcaacataa
    27721 ttagccactc cttttatttg ttttgtccgt aatacgcatt ttttccgtgt tttcgtaaat
    27781 aatgtttatc taaaatccgt tgaggcagct cttctgaaaa tgaattgagc tgtctggtaa
    27841 ataaattcat cttgcatact tcatctagta cgactgcgtt cattaccgca ctttgcgcat
    27901 ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac ccctggaatt gctaagggat
    27961 caattttgcg ttctttgaat gtttctatga taacttttcc tgtttcatat tcgtacccgc
    28021 tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac cgatccataa aaagtatctg
    28081 catgcgtcgt ccccatcgca ggaacatcca atcctgcttg tgcccagatc gtcgcccatg
    28141 tcgagtgagt atggacgatt cctccgattt gaggaaatga acgatataaa acagcatgag
    28201 taggcgtatc cgaagaggga ttgagtttac cttcaatgat atgattgtcc aagtccacaa
    28261 cgaccatgtc gtctgctgtc atctcttcat aactgactcc gctaggtttg ataacgaaat
    28321 atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt atatttgacg agtccgtgtt
    28381 taggcaagtc aagatttgct tggtatactt cttctttcag ttgttccagc attttctatc
    28441 actccttttt cttgcaattg atgaatcgct gttttttcaa tgtcgatccc ctcacggtaa
    28501 cgtctcataa atatctgata gcctgctatt tcttctgctg aaggtgtgat ggtgattcct
    28561 tggtcttgtc caaacacgtc ttctgccaag aagttttcta aagacttccc atgtcgatcc
    28621 atcatatagg ctgctaataa cgcgattccc cacgctccac cttctccggc tgtgtccatc
    28681 actgtaactg gtgcctccat agcagatgct agaatccgct gtccaacttc tggtgtttta
    28741 aaaatccccc catgccccac taaacgatca atttggacat tctcagattt caatatatcc
    28801 attccgattc tcattgcacc aaaagcactg gctaaatgga tccgcatgaa attcgctaaa
    28861 tcaaaacggc tacctggtgt acgtacaaat aatggccttc cttcatttac tcctgtaatg
    28921 ttttcaccgg aaaaataacc ataagaaagt aatcccccgc aatccgtatc gccttctagc
    28981 gctttgttga ataataccgt gaataattta tctctgctta tctccatacc caaagaattg
    29041 gtaaattctt caaaaatact gacccaagca ttgatatccg aagagcagtt attcgtatga
    29101 accatgccta ctagttctcc tgaaggtgta gtgaccatgt cgatttccgg atggacattt
    29161 tctagttctt tttctaatac gatcatagcg aaggcggaag taccagccga aatgttccct
    29221 gtccgtttgg ctacactgtt cgtagcgacc ataccggttc ctgcatcccc ttctggcgga
    29281 caaaccggga ttcctggtct caaatttccc gtagggtcga tcagataagc ccctgattcg
    29341 cttaacttgc cagcttcttc tcctgctact agaactttag gcaaaatgtg atccaagtca
    29401 atcgctacgt gcgctttttc gattaactga ccaaaaatat ttttcatttc ttgatggtag
    29461 gttttcgttg aaggatcgat aggaaacatg cctgatgcat ctcctacgcc tagtactttc
    29521 tcaccagtaa gctgccaatg gacataacct gcaagcgttg tcaaaaaatt aatttctttc
    29581 acatgttctt ctttattcaa aatagattga tacaaatgag cgatactcca tctctgcgga
    29641 atattgtatt gaaatttttc cgttaatatt cgttctgctt cttcagtgat tgtatttcgc
    29701 caagtacgaa atggtactag taactgttcc ttgctatcaa atgctagata accgtgcatc
    29761 attgcactga acccaatcga cccgattgtc gtaagattct cgccatagat ttcgaatact
    29821 tctgctgcca tttttcggta actgacgcgc agtcctttcc aaatatcttc taatgaatag
    29881 gtccagatac cattttctag tttgttttcc cactcataac taccagcggc aataggagta
    29941 aaagatgaat caatcaatac agctttgatc ctagttgatc caaactcaat ccctaaagat
    30001 gtacgaccat ctatgacatc taacatactt tgcgcattta cttccttcat aatgatttaa
    30061 ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta gtagcgctta caatgattat
    30121 tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa gaatacaata aaaaacactt
    30181 gttttgtacg tacataagtt ataaaatgaa attgcttaca aaatctgatc aaccgccttc
    30241 tcgaaattct agaaggagag aatacttatg aaaaaagaaa aaaaagtttc ctcatcgttt
    30301 atttattttt ttggcgcatt tggcggcatt ctatttggat atgatattgg tgttatgacc
    30361 ggcgccttac cttttttaca gcatgattgg ggtttggcag gtaaagcttc tttgatagga
    30421 tggatcacgt cttcggtcat gttaggtgcg atccttggcg gctctttatc tggtcttctt
    30481 tctgataagc ttggacgacg aaaaatgatt cttctttctg cacttatttt tatggctggt
    30541 tctgtccttt ctgcatctgc ccctcacaat ggttcttatt ttctgattgc agcaaggatt
    30601 cttctaggtt tagctgttgg cgctgcatct gccctagttc ctgcttatat gtctgaaatg
    30661 gctcctgcta gactaagggg aagattgtca gggatcaatc aagtgatgat tgcttctggc
    30721 atgctccttt cctatgttgc agattatttg ctaaaagtgt tgcctgaaac gatggcttgg
    30781 cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt tatttttcgg tgttttagct
    30841 ctaccagagt caccgcgttt tcttatgcaa tctggccgct tggaagaggc aaaaagagta
    30901 ttgaactata ttcggacacc aaaagaagct gaacaggaat tcgaacaaat ccagctgaat
    30961 gtcaaacaag aaaagacaac cggaacttct tggcatactt tgtttttaga aaaatatcgt
    31021 tccttagtca ttgccggtat tggtgtagca gtattccagc aatttcaagg agctaatgct
    31081 attttttact atattccgct gatcgtagaa aaagcgactg ggcatgcagc gagcgatgca
    31141 ttgatgtggc cgatcatcca aggaatcatt ttagttgctg gttctttgct ctttttggtt
    31201 attgcagaca agttcaatcg gcgaacttta ttgaaaatag gcggtagcgt gatgggactt
    31261 tcattcatcc taccagctgt tttaggaact gtactggatg ctcatacaaa ttcgttgttg
    31321 attctcttgt ttttatgtat ttatgtcgca ttttattctt gcacatgggc tccactgaca
    31381 tgggtaatca taggcgaaat attcccacta gctgtccgcg ggcgagcatc tggcctagcc
    31441 tcttctttta attggattgg ttctttccta gttggcttgc tttttcctgt gatgactgct
    31501 tctatgtctc aagaaatcgt tttcggcatt tttgggatca tctgcttttt aggggtttta
    31561 tttattcaag agatcgtacc agaaactcgt gggaaatctt tagaggaaat cgaacaaagt
    31621 gcatctaaaa aaacttatcc taaacgtatt tctatccatc attcttaaag aggttgtgac
    31681 agaagtggtc agcttcaaga aataagaagg aaccgcttag tttccagtca caaggaacaa
    31741 tttcaaaaaa cagcttctca tgtttgatga atattgtgac ttgtgaccag ttggtaggag
    31801 aacaccgttt agtggagagg tggtgaaaga aacgttcagc cttgagcgat gacaaaagct
    31861 ctcacaaaca ttgcgacgag taaccgtagg agcaataaga ggcggtaaaa acccgtctct
    31921 tatttttcgt tttctttcac tgtttcccgt tcaactagta caggttcata gagaatattt
    31981 ggctgtgcct gtcctttttc aatcgttgtg acgatccacc gtgcggcatc ttgtcccaac
    32041 ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc ctaaatggct taaagaagaa
    32101 tcgtctgttc ctacgatcga taggtcatct ggaatatgat agccattttc tgtcaactta
    32161 tctagcatta aactagctat ttgatcgtta tagcaaatga tgccatctaa agcttgttct
    32221 tctttcaacc gttgtactgt tttttcagcg attgtttctc gtgtttccgt ggtgaaagta
    32281 atgatatctt ccgtcaaaaa acgcacatta tactgttcac acgcgctgat aaaacctttc
    32341 atcctgtatt ttccttgaag atcgtcgatt tttgtgatga acaacagacg ctgatgtcct
    32401 ttttttatca aatattctgc tgccataaaa ccgactttca catcatctaa acaaatataa
    32461 gaggcattta actcttcata agcagcattg atcatcacca tcggaatgcc ttgttctctc
    32521 aaagcaacat aaagtgctaa gtttgggtta tactgattac ttttcgtagg ctcaacgatc
    32581 aaaccatcta ccccataagc aatcattttc ttcaaacact ctttctccaa ttcgtgatcg
    32641 ttattcgtac tggctaaaag caatgaatac ccagattctc gtaattcatt ttcgattcct
    32701 cggataatcg acggaaaaat ataatcagag agatatgtgg tgatgacacc gatcgttttt
    32761 gttctgttca atggtgtttt ttctttctta tagctgtcat ctacatatgt gcccgaccct
    32821 ttttctttac gcagatagcc ctcatttacc aacacagcga ttgcttgccg aatcgtatgt
    32881 ctgctgacgt catatatttc ttgcagcttg aattctgagg gaatggttgt atttacttcg
    32941 tattcctcac gaaggatctt ttgttttatc tcttctgcaa tatcttgata ttttgccatt
    33001 tgccttgccc ctttttattt gtacgtacat atatcataac gagtctgcaa ggtttattca
    33061 agcaaaaatc aaggtttctt gtgctcataa ggcatacgga acaggttcac caaattctgg
    33121 aaatccttgc tcattccacg taaagcattg cgcgttcgcg tgccgattag gattgtctaa
    33181 cggatcccct ttttggttct tctctggtcg agcatgatag atcaaaatat caaagcaccc
    33241 gtcttctgaa accgtaaacg agttgtgacc aggaccaaat tgcttatttt tttcagaaga
    33301 aacgaatact ggctccgata atttatgcca tgattctttt aataaaagat tcttgtgctc
    33361 atcagcccac agcaggccca tggcatagtt ttcatctgtt gcactaccag aatacgtaat
    33421 gaatattttc ccattgcgag taaggactgc tggaccttca tttactgaaa aaccccgttt
    33481 ttcccattca tactcaggta tagaaaggag gatctgcttc ccctttaacg tccaaggatt
    33541 atccatttcg gatagataaa gattagaatt cccagtaatt tcaggatctt gttgggccca
    33601 cacataatat agctttcctt gagaagagaa agttgttccg tctaaactga aagtttccct
    33661 tttcgttttt atttgtccct tttctatcca atctgtattc attggatcta tatgctgatt
    33721 ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt atagttttat gattgcttgc
    33781 agcaaagtaa atataccatt ttccattgat ataatggact tcaggtgccc agatcaattc
    33841 actcatcggt ccttcttcat gagcctgcca tacaatcagg cttgcagcat gttctaattc
    33901 attcagtgac tttgcccttc ttaattcaat cacttggtag cctggaaccg atcctgtaaa
    33961 ataataataa ccatctgtat gtttatatac ccaaggatct gcccgttgcc gaacgatagg
    34021 attattataa agtgtcttca tcataacaaa tttcctcaca ttttctttat tctaattaca
    34081 ttccaagagt atttttctat ccctacattt aattcgtcat gtttttctat tgaaaaatag
    34141 tttatttcag gttttatttt ttctggttct tcacgtgtat ttttgtccaa cagatcgcta
    34201 ctggttaatg tgtagtgctc tccttgatct agcaaattga aatcatgttc aatcgttatc
    34261 ttttgttgtt catttgtgcg attgatcaag aaaatagtca gctgattgcc gttatctacg
    34321 atcacactgt ccacataagg aacatttttt atcttttcac aatcataaac agggcaaatg
    34381 atttgttgtt tcaatacttg acctttagca tatttcgata agtgttgcat cacataataa
    34441 gttggattcc tccatgcttg tcctttagca tctgtcagta ttaaaggtat tgtgtttact
    34501 aacaatgatt gacaactaat ctttacgcga tctgcgtgtc gtaaaatagc cagccctaat
    34561 gaaccaaata ataatgtatc ttccatagta aagtaacacc aatcgatggg actaccgact
    34621 tcaaaatccc tatgtctttt ttccggttgg gcatgtacat tccattcatc aaacgctaga
    34681 tacattgttt tctcacttct caaatatcct tttacataat cacaagtagc aacgatttct
    34741 tcaatttgac gatcgaatcg ataagctttt gcaaggtatg tcgctgtatc atcacgctca
    34801 tttttaggag gtttagtcaa gtcttcttca tcatacttat caatatagtt atgcaaagct
    34861 aaataatcta cattctcata agcttcttct aatgcgatac gatcccattc agggtacgat
    34921 ggtaaacgtg gcgtagagct tccaactaag attgtttcaa ttgaatcatc cactaacttc
    34981 atgacttttg aggcttcatt tgctaaccta ccatattcat aagcactctt agcccccact
    35041 tgccatggac cgtctagctc attccctaaa caccataatt taacgccaaa aggctttttt
    35101 tgcccatttt ttattcttaa atcactccag aaggttcctt ttggaaaatt acaatattct
    35161 actaaatttt ttgcttcatc aattcctttt gttcctaaat tcaccgtgaa tatcggtttc
    35221 gcttgtacat gactgatcca tttaaaaaat tcgtggagtc caaaagaatt tggttctata
    35281 cctcgccatg ctaaatcaat tttttttggt cgttgttctt tcctacctac agtatccacc
    35341 caatcataac cagatgtaaa gtttcctcca ggatagcgaa tgacacctaa ttctaattcc
    35401 tttacataag agaggacatc ttgtctaaac ccgtcaccat cagctgtttc atggcccggt
    35461 tcataaatcc ctgtatagac gacacttccc atatgctcaa caaatgagcc aaacagccga
    35521 gaactgatct ctcctaccgt aaaatcctct ctgattttta ctttcacttc ttcgtccaac
    35581 agctttccct acctttcatt tcctatagtc aagataatag atacaagttt ccatacgcca
    35641 ccagcaaaga taaaaataaa aggagggaaa agaaatatga gcagtacaaa tcctaataac
    35701 aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata ctttccatgc actttgttca
    35761 aaaatttgta aaaatgtgat atctttgttt tcagaaatca catatatcca ggataaaaga
    35821 aacatggaat aaataagtaa aactacatat ccactaattg aaagtacgat tgtctgttct
    35881 tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag gaacaagttc
    35941 ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc tgatccctca
    36001 cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat agtaataaca
    36061 ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac cacaatcaac
    36121 tgtccataaa atttagtctc aactattttc atcttcctca ctctttcatt ccagacatag
    36181 ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat ggcagcattg
    36241 aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct gtaaataaag
    36301 ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat ggataaagca
    36361 agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta ggtttgctca
    36421 atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg cgtgctgcct
    36481 cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata ccaaatgcag
    36541 ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc aaattgactt
    36601 caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc aaaacgaaat
    36661 agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc gcaagagaat
    36721 caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt ttgatgtaat
    36781 ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag ctatctgcaa
    36841 atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa ataatcagcc
    36901 acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt cgaatgattt
    36961 ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt tctggtgcca
    37021 tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac attagtggag
    37081 atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc atagcccaat
    37141 tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc tgtcttaaat
    37201 tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga accaatcaga
    37261 ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat gttaaaaaac
    37321 tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg agagattccc
    37381 tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac agtcatgatg
    37441 gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg caagccgact
    37501 tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga aaaaataatc
    37561 gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca aaaacgattg
    37621 aatctggtat ttttagataa tgctactgct gctaataaac caacgataat ttgtgttggc
    37681 gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg ttcatcacta
    37741 aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa aatattcaaa
    37801 tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa taataaaact
    37861 aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca atatttggtg
    37921 aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat cttggtttaa
    37981 acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca cctatctgca
    38041 aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct gtataaagtt
    38101 ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat gagtgtctca
    38161 ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt attgatcgca
    38221 gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt cgataattca
    38281 gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa gtaggcagga
    38341 aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata tttcaaaaac
    38401 tcatagacag cttctttttt attattatct gtacttgccg gaatggcata tcctacgcca
    38461 tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt cacattataa
    38521 tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc aatggctagt
    38581 tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg accagtcttg
    38641 tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg agacgcaaat
    38701 tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat ccagttatat
    38761 aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact actatcagtt
    38821 aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc agtatctaat
    38881 ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac ctgcatgggg
    38941 acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt aaaatcagat
    39001 ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc gttttctaca
    39061 tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt agaagaaatc
    39121 gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc aattttgatg
    39181 tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc accatcagat
    39241 gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga ttctgctgaa
    39301 cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga gccaattaac
    39361 cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc tgaccacatt
    39421 ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta catttgttga
    39481 atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat caaatccact
    39541 catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa cagttagatt
    39601 catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat ttattacggc
    39661 tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg ctgaacgaat
    39721 ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg gataatagat
    39781 cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa cgtttaccaa
    39841 ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga ttaatagaca
    39901 gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag gagcaatctg
    39961 ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc attcatcaaa
    40021 ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc acatcgctgt
    40081 cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag aaagatcatt
    40141 tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt acgcttgatc
    40201 aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac ttccgcataa
    40261 aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct ctgctaaccg
    40321 tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt cattccctaa
    40381 acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta aatcactcca
    40441 ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat ctactccacg
    40501 agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc agttataaaa
    40561 ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca accttctagg
    40621 tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa aatttccacc
    40681 aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt cttggcgaaa
    40741 tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata ctgctcgtcc
    40801 catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac gcttgacact
    40861 actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg aataatacac
    40921 ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt atcgatttta
    40981 attaaccaaa atattaataa tgattaaaac aaataattta atataacttt tattaacaaa
    41041 tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta caaataaacg
    41101 aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag atcatccagc
    41161 tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga gtgagcagtg
    41221 ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa acagaaaaag
    41281 taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt tttccagaaa
    41341 aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac tatacaaatt
    41401 atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg aaagtggatg
    41461 aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg tttaccagcg
    41521 gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg gagatgttgg
    41581 aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg ccttcagata
    41641 tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga gattttgctg
    41701 atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa tacggcttgc
    41761 tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc ttgtcaaaca
    41821 tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc gaagtgaaag
    41881 acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt aattacgatc
    41941 aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt tataaaacaa
    42001 aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt ccagaaatca
    42061 tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca tgcttcgatt
    42121 ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat ttcattatat
    42181 gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag atctgcagtc
    42241 ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg tttcccttgt
    42301 cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa tttcgtgaag
    42361 aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc tctttctcca
    42421 gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt gttaaaatcg
    42481 ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc tggtttgata
    42541 tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc atcttcttca
    42601 tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc atcttttgtt
    42661 tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt agaatcttgg
    42721 ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa atatgctttc
    42781 ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt gacacctaat
    42841 tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct gtatgaagtt
    42901 ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag cattcagtag
    42961 acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc catagtgaat
    43021 ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg taaactcttt
    43081 ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga acagttttat
    43141 tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga gggtcttcat
    43201 cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg atgcgccaca
    43261 cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg atcgtatcca
    43321 tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca agggctggct
    43381 gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta gctttttgca
    43441 tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt tttgcttccg
    43501 taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc atatctccac
    43561 tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc acgcgaatcg
    43621 cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg cagtctttat
    43681 gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg actaacgaat
    43741 gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca ttgcttgttg
    43801 catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc agttcttgtg
    43861 cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg atacgagctg
    43921 cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt tctactaatt
    43981 ggcagtgcgc taaaggccct tccacttgga tcaatggttc atttgcaaaa actagttctc
    44041 cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac tccaaaaaac
    44101 cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg aacgtcagat
    44161 tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg tggttaaaag
    44221 gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct aattcccagt
    44281 atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg tcgtctgggt
    44341 aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac ccattataac
    44401 aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca tgttttacgg
    44461 atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat tggtatttaa
    44521 cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag aaattagcgt
    44581 tttctatttt tcttacttga taaaataagt agattttcct actacaatcg tagccgttaa
    44641 gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa tggcaaaagt
    44701 ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa acggatataa
    44761 cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac cttgcgttga
    44821 aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag aaagcagctg
    44881 tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt tttttattgt
    44941 atacattaga tatggagcag gttaccataa agatgattat tagtcgttat taaatagaat
    45001 ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg aattgtattc
    45061 cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct cgtgtcattt
    45121 ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc ttcgtattga
    45181 tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt cgatctaacg
    45241 aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact actttaggat
    45301 aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc atctcattcc
    45361 tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct ttcctagcat
    45421 taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt atcacttaat
    45481 agtataacat aatttcatga gaaaaaaata caattttttc ttgaccatct cgttacgtaa
    45541 aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa agaaaatgtc
    45601 agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg gactgctaaa
    45661 accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag tcgatcgtct
    45721 gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc aagaggtctt
    45781 agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa tgttgctgga
    45841 aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg atatgtacga
    45901 aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac aaaagttgaa
    45961 agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa aaaaggttac
    46021 agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa tgcaaactgt
    46081 tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag atgaaagctt
    46141 agcagaccgt atcttccaag ctcataagaa gtggttatct tacagctggc ctgaatatca
    46201 gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt tcactgcata
    46261 ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta tccaaaaaaa
    46321 ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta ataacaaaac
    46381 agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat tctttcacaa
    46441 gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg tattttcact
    46501 ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc ttccagatgg
    46561 tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg tccaatcacc
    46621 actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat atccgaattt
    46681 gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt tcctttctct
    46741 gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag ttgatgacat
    46801 agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa cgtgatgccc
    46861 aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga ttgttctcga
    46921 tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac tagaggtcca
    46981 tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat gatcaatcca
    47041 tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt ctgatagccc
    47101 gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat cccactgatc
    47161 aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct tttcgttttt
    47221 ccataactta actgtctggc atttgcatta gggatataat cgacttcttt catgaccgca
    47281 agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac acgtgaaata
    47341 gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac tgctgcttcg
    47401 ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg cattcaaaaa
    47461 actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa tatatatctt
    47521 ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta ttcaaccctt
    47581 gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa aaataattgt
    47641 tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct tttgatactt
    47701 tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa agaaaagttt
    47761 tattcctctt tcattattac caaagtctct tatttt
//
LOCUS       DASJTM010000012        43012 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJTM010000012 DASJTM010000000
VERSION     DASJTM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.012"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="U4B29_000640"
     CDS             591..1280
                     /locus_tag="U4B29_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2105610.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="U4B29_000641"
     CDS             1267..2475
                     /locus_tag="U4B29_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2105611.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="U4B29_000642"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="U4B29_000642"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN2105612.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4B29_000643"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4B29_000643"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN2105613.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="U4B29_000644"
     CDS             complement(6578..7273)
                     /locus_tag="U4B29_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN2105614.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4B29_000645"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4B29_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN2105615.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="U4B29_000646"
     CDS             complement(8472..8663)
                     /locus_tag="U4B29_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN2105616.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="U4B29_000647"
     CDS             complement(8684..9574)
                     /locus_tag="U4B29_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN2105617.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="U4B29_000648"
     CDS             complement(9561..10328)
                     /locus_tag="U4B29_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2105618.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4B29_000649"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4B29_000649"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN2105619.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4B29_000650"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4B29_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN2105620.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4B29_000651"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4B29_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN2105621.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="U4B29_000652"
     CDS             14899..15282
                     /locus_tag="U4B29_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105622.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="U4B29_000653"
     CDS             complement(15450..16208)
                     /locus_tag="U4B29_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN2105623.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="U4B29_000654"
     CDS             complement(16226..17044)
                     /locus_tag="U4B29_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2105624.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4B29_000655"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4B29_000655"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN2105625.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4B29_000656"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4B29_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN2105626.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="U4B29_000657"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="U4B29_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN2105627.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="U4B29_000658"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="U4B29_000658"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN2105628.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="U4B29_000659"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="U4B29_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN2105629.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="U4B29_000660"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="U4B29_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN2105630.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="U4B29_000661"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="U4B29_000661"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN2105631.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="U4B29_000662"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="U4B29_000662"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN2105632.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="U4B29_000663"
     CDS             27086..27385
                     /locus_tag="U4B29_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN2105633.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="U4B29_000664"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="U4B29_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2105634.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="U4B29_000665"
     CDS             27973..28209
                     /locus_tag="U4B29_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN2105635.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="U4B29_000666"
     CDS             28352..30325
                     /locus_tag="U4B29_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN2105636.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="U4B29_000667"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="U4B29_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN2105637.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="U4B29_000668"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="U4B29_000668"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN2105638.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="U4B29_000669"
     CDS             33204..34496
                     /locus_tag="U4B29_000669"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN2105639.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="U4B29_000670"
     CDS             35283..36146
                     /locus_tag="U4B29_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2105640.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="U4B29_000671"
     CDS             complement(36275..37897)
                     /locus_tag="U4B29_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2105641.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="U4B29_000672"
     CDS             38146..38667
                     /locus_tag="U4B29_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2105642.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="U4B29_000673"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="U4B29_000673"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN2105643.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="U4B29_000674"
     CDS             40748..41566
                     /locus_tag="U4B29_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN2105644.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41669..41866)
                     /locus_tag="U4B29_000675"
     CDS             complement(41669..41866)
                     /locus_tag="U4B29_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105645.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42035..42613
                     /locus_tag="U4B29_000676"
     CDS             42035..42613
                     /locus_tag="U4B29_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2105646.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42708..42845
                     /locus_tag="U4B29_000677"
     CDS             42708..42845
                     /locus_tag="U4B29_000677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105647.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT        13060 a         8249 c         8499 g        13204 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41581 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41641 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41701 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41761 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41821 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41881 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41941 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    42001 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    42061 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    42121 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    42181 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42241 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42301 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42361 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42421 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42481 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42541 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42601 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42661 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42721 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42781 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42841 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
    42901 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
    42961 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aa
//
LOCUS       DASJTM010000013        42955 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJTM010000013 DASJTM010000000
VERSION     DASJTM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.013"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            110..751
                     /locus_tag="U4B29_000678"
     CDS             110..751
                     /locus_tag="U4B29_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2105648.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            794..1486
                     /locus_tag="U4B29_000679"
     CDS             794..1486
                     /locus_tag="U4B29_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN2105649.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            1483..2385
                     /locus_tag="U4B29_000680"
     CDS             1483..2385
                     /locus_tag="U4B29_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2105650.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2543..4249)
                     /gene="efbA"
                     /locus_tag="U4B29_000681"
     CDS             complement(2543..4249)
                     /gene="efbA"
                     /locus_tag="U4B29_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN2105651.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4439..5062
                     /locus_tag="U4B29_000682"
     CDS             4439..5062
                     /locus_tag="U4B29_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105652.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5512..6561
                     /locus_tag="U4B29_000683"
     CDS             5512..6561
                     /locus_tag="U4B29_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2105653.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6558..7439
                     /locus_tag="U4B29_000684"
     CDS             6558..7439
                     /locus_tag="U4B29_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105654.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7442..8212
                     /locus_tag="U4B29_000685"
     CDS             7442..8212
                     /locus_tag="U4B29_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2105655.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8491..9345
                     /gene="aroE"
                     /locus_tag="U4B29_000686"
     CDS             8491..9345
                     /gene="aroE"
                     /locus_tag="U4B29_000686"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN2105656.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            9523..10686
                     /locus_tag="U4B29_000687"
     CDS             9523..10686
                     /locus_tag="U4B29_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2105657.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            10803..11213
                     /locus_tag="U4B29_000688"
     CDS             10803..11213
                     /locus_tag="U4B29_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2105658.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            11387..13615
                     /gene="topB"
                     /locus_tag="U4B29_000689"
     CDS             11387..13615
                     /gene="topB"
                     /locus_tag="U4B29_000689"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2105659.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            13786..14070
                     /locus_tag="U4B29_000690"
     CDS             13786..14070
                     /locus_tag="U4B29_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105660.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            14230..14466
                     /locus_tag="U4B29_000691"
     CDS             14230..14466
                     /locus_tag="U4B29_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2105661.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            14463..14862
                     /locus_tag="U4B29_000692"
                     /pseudo
     CDS             14463..14862
                     /locus_tag="U4B29_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(15529..16473)
                     /locus_tag="U4B29_000693"
     CDS             complement(15529..16473)
                     /locus_tag="U4B29_000693"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2105662.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(16769..17713)
                     /locus_tag="U4B29_000694"
     CDS             complement(16769..17713)
                     /locus_tag="U4B29_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105663.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            17934..19148
                     /locus_tag="U4B29_000695"
     CDS             17934..19148
                     /locus_tag="U4B29_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2105664.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19170..21185
                     /locus_tag="U4B29_000696"
     CDS             19170..21185
                     /locus_tag="U4B29_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN2105665.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(21268..22641)
                     /locus_tag="U4B29_000697"
     CDS             complement(21268..22641)
                     /locus_tag="U4B29_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2105666.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(22667..22951)
                     /locus_tag="U4B29_000698"
     CDS             complement(22667..22951)
                     /locus_tag="U4B29_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2105667.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(22953..24968)
                     /locus_tag="U4B29_000699"
     CDS             complement(22953..24968)
                     /locus_tag="U4B29_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2105668.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(25147..25452)
                     /locus_tag="U4B29_000700"
     CDS             complement(25147..25452)
                     /locus_tag="U4B29_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN2105669.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(25541..26572)
                     /locus_tag="U4B29_000701"
     CDS             complement(25541..26572)
                     /locus_tag="U4B29_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2105670.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            26779..27654
                     /locus_tag="U4B29_000702"
     CDS             26779..27654
                     /locus_tag="U4B29_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2105671.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(27806..28648)
                     /locus_tag="U4B29_000703"
     CDS             complement(27806..28648)
                     /locus_tag="U4B29_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2105672.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(28740..30617)
                     /locus_tag="U4B29_000704"
     CDS             complement(28740..30617)
                     /locus_tag="U4B29_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2105673.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(30614..32035)
                     /locus_tag="U4B29_000705"
     CDS             complement(30614..32035)
                     /locus_tag="U4B29_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2105674.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(32038..35427)
                     /locus_tag="U4B29_000706"
     CDS             complement(32038..35427)
                     /locus_tag="U4B29_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2105675.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            35684..37075
                     /locus_tag="U4B29_000707"
     CDS             35684..37075
                     /locus_tag="U4B29_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105676.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(37148..37795)
                     /locus_tag="U4B29_000708"
     CDS             complement(37148..37795)
                     /locus_tag="U4B29_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2105677.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(37803..38489)
                     /locus_tag="U4B29_000709"
     CDS             complement(37803..38489)
                     /locus_tag="U4B29_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105678.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(38495..39568)
                     /locus_tag="U4B29_000710"
     CDS             complement(38495..39568)
                     /locus_tag="U4B29_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105679.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            39790..40464
                     /locus_tag="U4B29_000711"
     CDS             39790..40464
                     /locus_tag="U4B29_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2105680.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            40469..41809
                     /locus_tag="U4B29_000712"
     CDS             40469..41809
                     /locus_tag="U4B29_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2105681.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(41958..42944)
                     /locus_tag="U4B29_000713"
     CDS             complement(41958..42944)
                     /locus_tag="U4B29_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN2105682.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        13210 a         7801 c         8251 g        13693 t
ORIGIN      
        1 tttggtaagg gagctgtttc aaaaatggta aagagcggaa atgttgcgaa tatcagttat
       61 ttaatgatat gatattcgga aaaagaaatc tcaaagggag gtaactttta tggggttgaa
      121 tgaatttatt ttaggattga acaatttaga gacgatcaca agggcacctg gattttttaa
      181 atatacagag catacagttg cagcacattc gtatcgtgta gcatctattg cgcaagttct
      241 aggagacatt gaagaagcga atggacaacc gatcgattgg aaactgctct atgaaaaatc
      301 attgaatcat gactacacag agcgttttat tggtgatatc aaaacgccgg tgaaatacgc
      361 aagcaaagag ttaagaggga tgctgcaaac agttgaagaa aagatgacgg acgaattcat
      421 caaccaagag attccagctg agttccaaga agtttatcgc cgtcggttgt tcgaaggaaa
      481 agatgaaacc gtagaaggag aactattagc catttcagat aaggttgacc ttctttatga
      541 atcttttgat gaaatcatca aaaataaccc ggaaaaagtt tataaggaga tgttcattga
      601 ggcagtgact acgattaaaa gctttgaaca tcggccttct gttcagtatt tctttaaaga
      661 gatatttccg gaactattga accaggaatt ttatggaaaa gaggcgtttt tagaaaccgt
      721 ctcaacgatt gtaaagaaag aagaaaaata aaaatgatat gagatgcagg gggaagtata
      781 gttgaaaaag aatatggatg tcgaatgggg ctattctgga gacaaaggtc cggaaaattg
      841 ggcgagttta tgtgattggt ttgcgagagg tgcggaattt ccattgcagt cgcctattca
      901 tttgaccaag ggggaagaaa ccaaaataga aggagacacg ctttcctttc attatcaaaa
      961 gcaacggttt actgacaagg aattcaaaaa cacgattcat ttagtacctt ttgaccaact
     1021 cagttatgta gagttcaaga aagaacgcta ttatttgaca gatattcatt tccacttgcc
     1081 ttctgaacat atcatcaacg gaaaacagga agatattgaa tttcattttg ttcatatgaa
     1141 cagcaaaaaa gagaatctag ttgtgggtgt catgtaccag ctaatggtac aggaaggttg
     1201 gctgtataat caagaaaatg gggaaagttg gaacttagaa aaacatgagc attgggtaaa
     1261 tccggatgtg ttctttccgg agcaaaaaag ccatttccat tatattggct cattgacaac
     1321 tccgcctacc agcgggccaa tccaatggtt tgtaatggat catgtaggaa aactgaacca
     1381 agagtttctt ctccccttcg atggtcagta tgctcgaccg aacaaccgac caatccagcc
     1441 cttgaatgga agagaaattt attatttcga ggagtttgag caatgaatat tcagcagatg
     1501 aaatacgtgg tcgctgtagc aaacaatggt agtttcagag aagcggcaaa gaaaatgttt
     1561 gtctcccagc caagtctttc agctggtatc aaagaattag aaaccgagtt agggatcacg
     1621 ctgtttactc gaacgaatcg aggggcatat ttaacagaag aagggcaaga cttcttaaat
     1681 cgtgccgaga aaatattggt ccagcttgat acattagaaa ctcactatct aacgagtgaa
     1741 aagagagaca atttttcgat cgctgcccag cattatgatt tcttaggtcc tttgaccgca
     1801 cagctactcg aggaattcaa agacgagatc aaacaatttc ggatcatgga gacgacaaca
     1861 gcgagagtga tcgaagaagt caaagagcag cacagtgaga tcggcattat ttatatgaat
     1921 gagcataatc aagctggtat cagaagatat ttacaacaag gagagctaag tggccgcagc
     1981 attggaaaat tcaaaacgca tattttttta aggcaaggac atccattagc tgaacacgca
     2041 gagatcacca aagaagaatt gttatcttat cctcaagttc gttttacaca ggatggaaat
     2101 aatttcccgt atttttatga agaccttatc gagatccctg atcaagaaac agtgatttat
     2161 acgagtgatc gaggtacttt gatgaatatc gttcttgaaa ctgatgctta cgcttcagga
     2221 tctggtattg tgattggagg gattcgcgag cacttgcggt taatcccttt ggcagaaggc
     2281 gaactaaatg aattttatat catttattcg gcgaaacgtc cgctcagtga aatcgctcaa
     2341 agatttatca aagagcttac tcggttactg gaccagcaga gctaagaaaa gcagatgaga
     2401 acgctgttta ttgaacaccg ttgatcaaaa aagaggggtt gtgacagaat tttgtcacaa
     2461 ctcctctttt tgtagcaaac aatttataac taattgctgt tgtgggaaag tacgcaaata
     2521 tgattctaga aacactcatt gattacctgt tttgtttcat agctgtaatt ttttcttctt
     2581 ctggtgtcac gataatcgtc ttttggtttt cataaataac ataaccaggc ttggcgccat
     2641 ttggttttcg gatatgcttt acctgtacaa gatcaaccgg cacttgggca gagtatcggt
     2701 attttgaaaa ataggcagct aactcggcag cttctgtaat tgtttcatct gagggcttgt
     2761 cacttttaat gatgacgtgc gaaccgggta tattttttgc atgaagccaa tagtcggttt
     2821 ttttagctgt cttcattgtc agctggtcat tttgaagatt gtttttacca acaagaatga
     2881 gcgttccgtc agaagaaaag tattgatcag gctgagatgg tttcttacgt ttttgttttt
     2941 ttaagttctt ctttttcagg tatccttctg aagtcaattc ttctttaatt gcttcgatat
     3001 ccattgggcc ggcaatttcc agttgagata agacagattc aagatattgg atttcatctt
     3061 tggcttcttg gatttgttcg ccaatcagct taacagcgtt cttcagcttt tggtatcgat
     3121 ggaaatattt ctgtgcattt tgatttggtg tcaatgccgg atctagtgct attttgattg
     3181 gccgatcttc ttcataataa ttaggtaaaa caacttcttt tgcgccgcgt ggtacttgtg
     3241 tcagaaaagt agtaagtagt tcgccatttc tacggtaatt ctcagcgttt tcggattctt
     3301 tgagtgtttg ttcacgtttt ttcaatttat ttttatttcg ttttagttca ttttcgattt
     3361 ttcgaatcag ttctccgcct tgttgttttg cccgatcttt ctctgctttt tcgtgataat
     3421 agctatctaa taattgactg agtgttggat aagctgttac agcagatgcc tgttcggaca
     3481 atacctgata agcaatcggt gtgaaaaaat ctttattttc cgtttcgtaa aaagtaggaa
     3541 taggctgatg gctgatggct gagaaaaatt cgtcccatac aaccatcttt tcattaggac
     3601 gttccagtaa tcttttggtc agctcttgag cgatatcgaa accgattccc tgaaactgac
     3661 gttggattgc ttttggatct agttcagttt gggaaagccg ctgaaagatt ttttctttct
     3721 cggagctaaa tgggttcaag acctcttgtt ttggcggagc aacatattct acaccaggaa
     3781 gcaaagaacg ataagtgttt tgagagcttc caatgtgttt gatcgcatcc agtatttttc
     3841 cagtttctcg attaactaag acgatcgtac tatgacgtcc cattaattca acgatcaata
     3901 tgatattttg tagatcaccc aactcgtttc gtttagcaaa gtggaaatgg atcactcgat
     3961 cattttcgat ttgttcgatg tcttctaaaa tagcgccatc caaatatttt cgaagcatca
     4021 tcacgaagtt aggcggatta tcaggattct gataatcgat ttgggtaatc tgtacacgag
     4081 cataacttgg gtgcgcagat aataataatc gatggttttt gccgcgtgaa cgaatcacca
     4141 gcacgatttc attttcataa ggctgatgga ttttcgagat ccggccagat agtaacgttt
     4201 cacgcaattc gttgaccatc gcgtgggtaa atacaccatc aaaggacatg gttcattcct
     4261 catttctttt cataagtata ttcattataa cgaaaaaaag aagaaaaacc aatttggccg
     4321 aatagtaaag caaaagtcta gaaaaatctt ggactaatta cataaaaaac agtgtttcta
     4381 tcgttgaaat ccaaattttt aggtataata aaaaatagga gaaaaagtag aaaagaaggt
     4441 gtttgttatt tgggaagtag ttaaatcgag agtacaagaa atgaatgaac atttctcagt
     4501 gtcattcgca gaaaatccaa gtaagtattt tcgacgtctt gtttttctgc tcatcttatc
     4561 attgctgtta acagttgcaa tgtttttatt gccggaaaaa atccgagaag agcaagtaaa
     4621 tagtctcgtt tattcgtcca taccaaccag caaattgaaa ccttttgatt acagcaaagc
     4681 tgatcagaaa atcaaacaaa gccgggcgat atcggtgatg ttcagcaaac cgaacgggaa
     4741 aacctatcag gacatgcttg atgtttttaa tgatcctgag cttatggaag aattgaatcg
     4801 accgatcttt tattatccga tcgtttatga tgtacatgaa ctggaacaaa aatataacat
     4861 acgaacggat gaagtgacat ttattttctt tgatggagga aaagaagcaa atcggataac
     4921 tgcgggtaag ggaggaataa ctgattttga taaggagttg atccctgaac tgaatcgact
     4981 tccgttagcc aatatcaaac aattagaaga agaattggat agaacgggag taacttccca
     5041 aaattccgag attgctaatt gagacattgg cgcgatgaaa cggtgggtct atatagcgct
     5101 atttttaatt atcagaacaa gtgatgtttg atcttcaaaa taacaaataa aaaattaaaa
     5161 aatatagttg acgaaattca aaaattcgat tagacttaaa tcaattctta aggaaagggt
     5221 gacctgtaat gaacttatca aaacgtattt cagcagaaaa caaaaaaaat tggcgcagct
     5281 ggcgtagata agttgtatta atggtgagac tttcccatag atacaacttt ttagagtgca
     5341 tttctaaagc ttgtatctat gctagacatg aacattgact gccgcataga ttagaatcta
     5401 tgcggttttt tgtgtaaaga caatgaccgc tttggccatt gtcttttttt gtagactgga
     5461 tacttgcctg attataataa ataaaaagat tgactttgca ggaggaaaaa aatgaaaaac
     5521 aaacgattaa taacagttgt agtaattatt ttagtttatt tagcaggtgc atttatttat
     5581 ggaaaagcct caggcgaaaa agaaagtact tcgacgaacc agacaaaaaa aacaacagtt
     5641 gttggggtat tgcaatacgt gagccatccg gcattagatg agatttatcg aggaatcaaa
     5701 gatggtctga aagaagaagg attaaaagaa ggagaaaatc ttgagatcaa atttcaaaat
     5761 ggacaagccg atcagagcaa gctagccaca atgagtcaac agctagtaca atctgatcca
     5821 gatgccttga ttggaatcgc aacacctgcc gctcaatcat tagcaaacac gacttcatcg
     5881 atcccagttg tgttaggtgc tgtaacggat cctgtggggg ctggattagt cgcttcttta
     5941 gagaaacctg gtggtaatgt aacaggtgtt tctgatcagc cgcctgttac ttcacaaatc
     6001 aagcttgctt ctgatttgtt gccaaatgca aaaaaagtcg gaatcttgta tgcttcttca
     6061 gaggataatt caaaatacca agtagcacaa gcagaagaag ctgcaaaaaa agaagggtta
     6121 gaatcagtaa aatatgcggt gccttccact aatgagattg cacaaacggt tcaagtgatg
     6181 agccgacaag tcgattttat ctatgttcca ttagataata ccattgccaa tgcgatgcaa
     6241 acagtggtaa aagaagcaaa caaagcaaat atccccgtca ttccatctgt ggatacaatg
     6301 gtggaacaag gtggactagc aacaatcggg atcaatcaat atcaattagg gcttcaaagc
     6361 ggtaagatgg cggcagaact tgcgagtgga aaagaaaaac cggaaacgac gcctgtttat
     6421 acatttgatc aaggggatac agtgatcaat caatctcaag ctgatcgctt aggaatcatt
     6481 gttccccaat cgatgaagga aaaagccaaa atcattacag atgactcgca gcaagaaaca
     6541 tctaaggagg atgacaaatg atcgtatcag ctatttcaca aggcatgctt tgggcaatat
     6601 taggattagg gattttcatg acttatcgga tcttagactt tcctgatatg acgacagaag
     6661 ggtccttccc attaggtgct gctgtctgtg tgacagctat tactcatggg gtagcgccga
     6721 ttgtagctac attattaggc gtaggtgctg gcatgatagc aggactcgtt acggggttac
     6781 tttatacaaa aggaaagata ccggttattt tagcagggat tttagtgatg tcaggattga
     6841 actcagttat tttgtttgtg atgcgaacac caaatctttc tttattgaat catccgattc
     6901 tacaagacag cttccatcgc tttgggttac ctgattactt tgacacagtc cttcttggct
     6961 tcatctcttt gggcatcttg attgctgtac tgctattctt cttcaataca gatttgggtc
     7021 aaggctatat tgcgacagga gacaacgaaa cgatggctcg gtcgttaggg ataaaaaccg
     7081 atcgtatgaa aattttaggt ttaactttgt cgaacggtgt cattgcatta tccggtgcgc
     7141 tgattgccca gaacgacgga tatgcagatg taaataaagg gattggcgta atcgtcatcg
     7201 gtcttgcttc catcatcatc ggtgaagtga tcttccaaga attgacgcta gcagaaagat
     7261 tgattgctat tgttgtcggc agtatcattt atcagctatt gattttagta gtaatcaaac
     7321 tagggtttga cacgacttat ctgaagttgt tctccgctat catcttagct atttgtctga
     7381 tgatcccgca attgaagaaa gcactgaact taaaaacggg ttttgagaag gaggcatagt
     7441 aatgactgat atattgacaa tcaaaaatgg cagaaaagtc gtaaacaatg gaatgaatga
     7501 agagaaagta ctattggatg aaattaacct tacaatcaga gaaggggact tcatcactgt
     7561 ccttggcggg aatggtgctg gaaaaagcac actgttcaat acgatcgctg gtacgcttca
     7621 actcagtcaa ggatcgatct cttttaaaga tcagctgatc acaaaagatt cagaagaaaa
     7681 aagagcacga ttcctttctc gagttttcca agaccccaag atgggaacgg ctcctcggat
     7741 gacagtagca gaaaatttgc tacttgcaca aaaaagaggt caaaaacgga aattccgttt
     7801 acgtaacttg aaaaatcaaa aagaagattt tttcagacta tgtcaagaag tcggtaatgg
     7861 cctagagcaa cacttggata ctccagcggg tgaattatct ggtggtcaac gccaagcatt
     7921 aagcttgctg atggcgacga tcacagctcc tgagttacta ttattagatg aacatactgc
     7981 agctttagat cctaaaacag ctaaagcatt aatgcgatta acaaatcagc ggatcaatga
     8041 acaaaaattg acttgtctca tgatcacgca tcgaatggaa gacgccttga aatacggaaa
     8101 tcgtctgatc gtactccaaa aagggaaaat cgttcaagat atttctgaaa aagaaaagca
     8161 acatttgaca atgggcgacc ttttgcagtt ctttgaagaa gtagtggatt gatttcgaca
     8221 gaaaaattcc tttcagtaga aggacttgaa aactcattga catcttcctt tgattcatcc
     8281 tatacttaaa acaataaaca attaaacata tttgggtgtt ttgtaggatc acgtagtcaa
     8341 tgattttaac aattaaatag aatattcttt gctctgacaa gtaaacgatg agaaaagaat
     8401 ttgctacttt gccacctaga cgtttcgtac tttttcgtat caaacgctaa gtggcttttt
     8461 ttattgaagt aagaacaaag gaggaataag atgatttctg gacaaaccaa attagctggg
     8521 ctctttgctg atccggcttc tcacagttta tccccattga tgcacaatac tgcttttgaa
     8581 gcaaacaaca tcgatgcggt gtatttagct ttccaagtaa atccaatgaa tttgaagcaa
     8641 gcagtcgaaa gtattcgaac ttttgatatg ttaggtgtaa atctttcgat gccgaataaa
     8701 acagcggtta ttccttatct ggatgaggta agccaggaag cgcaattgat cggagcagtg
     8761 aatacgatcg tgcacgaaaa gggtcgtttg attggctata atactgatgg tgcgggcttc
     8821 atgcaatccg tgcaagaagc tgggatttcg atcaaaaaga aaaaaatgac tctactggga
     8881 gctggaggtg cagcaaaggc agtcactgtt caagcagcgt tagacggcgc tgaggaaatc
     8941 gtagtttata aacgaaacaa ccaaacattc gctcaagtaa aagaaacatt cgaacaactc
     9001 agctatcgaa cttcttgtaa gataaaagtt cgtgactttg ccgatacgca tcaattaaga
     9061 aaagacttga aagaaagctg tcttctagtc aatgcaacag atatcgggat gggaaagaaa
     9121 atcaatcagt caccgctctt agatataagc atgatgcatg agaatctagc ggtttttgat
     9181 ttgatttatg cacctagaga aacacgttta ttaaaagaag caaaaaaagt aggagcaaaa
     9241 ggatacaatg gacttggcat gttgatctac caaggagcaa tcgcgtttga attatggaca
     9301 aaggaaaaga tgccgatcca tgtgattgaa aagctgtttg attaaaaaag gataatagat
     9361 aaagatcttt tattctcgca taaacggtct caagaaaagt aatcctttat gtatcgaagc
     9421 atttatttcg ttatttttta ttaaattatc aaaaaatgat aggcttttgt cgattggtta
     9481 tcgtatcctt tatttatcag taagaaacga ggaggaacca caatgagtcg gatggatcgc
     9541 tataaggaca tccataagaa ggcgaaacct ctgaagcggg atacggggag tatttttgct
     9601 agaagagaac gaaaacgaac gcgtatggaa aatgaggaag atgctacccg tatttatgaa
     9661 agagagcagc attcatttga acatttgaac caatacgagc aacagatata cagagatgca
     9721 ccaagagcag aagaacaaca aaaaaataaa aaatttttca atcggcaaaa gaagaacaaa
     9781 ccgttaaaac ctcgaagaaa gcgatcgtgg aaaaaaatca ttctatcgat tttgcttgtt
     9841 ttgatccttt attcaggtat ctcatttttt attggtaaat atgtagctga acatgacaca
     9901 agcttagagg cagcgacttc agaaaccttt catgggcaaa aagcgacgaa tggtgcgcat
     9961 aatattttga tccttggaag tgatacacgc ggagaagatg ccgggcgagc cgatacgatc
    10021 atggtcttac agttggatgg tccttcccat aagccgaaac tgatttcttt tatgcgtgac
    10081 agcttcgtta cgatcccagg ggtaggacaa aataagatca attcagctta tgcatacgga
    10141 ggagccgatc tagtgcgtca aactttagta gaaaattttg gaatcgattg tcaatactat
    10201 gcgaaagtcg atttcaaatc gtttgaaaaa gtgatcgatg ccttattcat gaacggagtg
    10261 aagatcgatg cagaaaaaga tctgaatctg gatggtgtag atattaaaaa agggggccag
    10321 aagatggatg gacatgttct tttgcagtac gcacgtttcc gaatggatga acaaggagac
    10381 tttggccgtg tcagacgcca acagcaagta atgaacgcta ttttcagcca attgaagaat
    10441 ccattgaatc tgatccgtgc accttatgca gctggtaaag ctttaggata tacatccaca
    10501 gatgtcactg cttggttctt gacgaaaaac ctcttttcga ttgcacgagg agctggtggc
    10561 attgatcgaa taagtgtgcc agtagacggt tcgtggaatt atgggcaaag tgattatgct
    10621 ggcagtattc ttgaaattga taatcaaatg aatcaatctg ctattacaga ttttttgaat
    10681 aaatgatcat aaaaaagatc aagaagaagc tgcgcaaggc ttttgtcatg aatcaatcaa
    10741 acgattcact cgtaaaaaac gctttcttat ggtagattta accaagagag gagctgttta
    10801 cattggatat ctatctgaac ttcaaaaatg agtcgcgtga agccatcgct ttttatgaaa
    10861 aagtttttca gactgaagca tcaggtatca ccacatacgg tgaaatgcct ggaatagaac
    10921 aaatgaactt gccagaagaa gcaaaaaatc taatcacgaa tgcacggctg aacatttatg
    10981 atacattagt tatgtttgca gatacgccag aaggaattgg tcctgaatta gttgtgggaa
    11041 ataatgtttc tttagtcatc cagacaagaa atgaagaaga aacgaagcgc ctatttgaaa
    11101 gcttagctga gggtggtaag attgagatgc cactggatgc tgttccgtgg gctcagttgt
    11161 atgggatggt aactgatcga tttggcatca actggcagat taaccaagag tgacagctaa
    11221 agatgaatat tttctggaaa catcaagcag caaacggatc atcgtttgtt tgcttgatgt
    11281 ttttgtgtct atttcgacta tacggtatct tttttttatt gccagatgcg aaaatatgtt
    11341 ccataataga gaagtccgca aaaaattaag taacggaggt cagaagatga agacggtgat
    11401 actagctgaa aaaccttcac aagcaaaagc ctatgccgaa accttccaac agcaaaagcg
    11461 gcatgaaggg tattatgaaa tcagcgatcc attgtttgct ggtgaagtaa ccatcactta
    11521 tggttttgga catttggtag atatggttgc tccaggagct tatgaagagc gttgggcaaa
    11581 atggtcttta gaaaatttgc ctattttccc agaaaccttc cgttacgaag ttccaatgga
    11641 caaacgtgca caattttcga tcgtaaaacg agaattgcag tcagccgata ccatcattat
    11701 tgcaacagat ggtgatcgag aaggtgaagc catcgcgtgg tcgattattc gacaagcaaa
    11761 cgcattttct aaggaaaaga actatttacg tttatggatc aattctcttg aaaaagaagc
    11821 aatctatgaa ggttttaaga atctccaacc tggaaagaac tattttccaa aatataaaga
    11881 agcgcaagca aggcagaacg ctgattggct gattggaatg aatggaagtc cattatatag
    11941 cttgctactt caacgaaaag ggattgatgg cagtttttct cttggtcgtg tacaaacacc
    12001 gacactctat atgatttatc agctgcaaga agaaatcaaa cacttcaaaa aagaaacgta
    12061 ctttgaagga gaaggtttaa ttacgacaaa caaagggcag ttttctggaa aaatcgtacc
    12121 aaaaaaagca tttaaaaccc aagaagagtt aacgaaggaa atagaaaaac ttggagcgca
    12181 tttaggtaag caaaacggac ggattcttga agttacgaaa aaggaaaaga ggttgcatag
    12241 tccacgttta ttttcgctat ctagtttgca gacaaagatg aatcagctaa tgaaagcaag
    12301 tgccaaagct acacttgatg ctgctcaagg attgtacgaa agcaagtttc taagctatcc
    12361 aagaacggat tccttttaca ttacagaaaa tgagcatcag tatctggtta aacatctgca
    12421 aaattataaa aaatttttag gaatagaaga agtacagaca actgagatca aagcaaagaa
    12481 acgatatgtc gatgcgaaaa aagttcaaga acatcatgca attattccga caaagacgat
    12541 tccgactcct gcacgctttg cgaagctttc caaattacag caagcaatct atttgcaagt
    12601 tttgcgcact actgtggcta tgtttgctgc agattatatc tacgaagaga cgaccgtaat
    12661 gacgggcgta cagcaattaa agctaaaaag catcggaaag atcccattac agcaaggttg
    12721 gcaggaaatc ttacgcatca agtcaaaaaa gaaagaagct cagactcttc ctcctatcgt
    12781 agaaaatgaa gaagcagaag tagaactgaa aagtatggag aaagaaacac aacctccaaa
    12841 accctatacc gaaggaacgt tgatcacagc aatgaaaact gcgggaaaaa cgctggataa
    12901 tgatgaagcg caagaaattc tcaaagaagt agaaggaata gggacagaag ctacacgagc
    12961 caatatcatt gaaaatctta agcagaagca ttacatcgaa gtcaataaaa acgaaataac
    13021 tgttacgcca aaaggaatca ctctttgtaa ggcagtagcc aatgaaccat tactgacgag
    13081 cgcagaaatg actgcgcgct gggaagggta cttgagaaaa attggtaaac aagaggggac
    13141 accatctgtt tttttagaga atatcaaaaa attcatccta catttattgg cagaagttcc
    13201 taatcaaatc gacaaggtag acttttcaga tgagattagc gggacacgtc agataaaagc
    13261 gatggagaag aaaaacgatc aattaggaag atgtccaaaa tgtaaaaagg gaatagtcat
    13321 gctttatcct aaaatcgcta catgcttgaa tccggaatgc gacttcaaat tgtggccaac
    13381 agtagctaaa aagaagctga ctaaaacgaa tctcagagat ttgctttcaa aaggaaagac
    13441 aagtaaagtt gtcaaaggtt ttactggtaa aaaagggaaa ttcgatgcag ttttagtatt
    13501 aaaagaagat atgacaatcg gtttttcttt tccatggatc gaaaacagtg gaacaaaagc
    13561 agttgatgga aacaaagaaa catcaagaaa aaataatgaa aacgaagtag agtaaatgaa
    13621 aacaatgaaa tagcggaata tttattccga aaagtgagaa tataaatacc aataaaacta
    13681 aaaaatatca gtgaatcaag caaaaacgtt ggggtaggct gattttatat agatagcaag
    13741 gagttttttt atttgaaaaa agtgcattca tttttaaatt atcttatggg agcaatcacg
    13801 attgtggctg tgtaccaagc atttctaaag tatcctgatt acgggacgaa gttgttgtca
    13861 ttgacgcaca atcaaacagc atattcacca acgttgattt tatcgacttt gactgcagga
    13921 gctaaaagcg ctacttattt ttttcataaa acgacaagca tgctaaagcg aaagaaacag
    13981 cgaaaacaat tgttggtggt ggtagcggct atgatcatta tttattttct accagttatt
    14041 acacaagtat ttcatcaata ttttggttaa gaatacgaga gtttgtgaca aaagtcatgt
    14101 cacaaacttt tttctttgtc attttttcgt aagaaaaagc aaaataaaag tatatacatt
    14161 gattaaaagt atatacttgg atataatgta cttactatag aatgagaatg gtatagaggg
    14221 tggcgaaata tgaaaaaaat gaaaaagtat aaaattagaa aatctggcaa tagcgatgta
    14281 acaactatcc caccagaagt aaaagaattc atgggtgtgc agacaggtga cgcaatcagt
    14341 tatgtttttc aatctgatgg ttctgtacga atgatcaaag cacaagaaga acctgatatt
    14401 gattcattag ttgattcgat catgaatcaa tatgaagacg cgttaaaaga tttggttgat
    14461 ttatgaggta tctttcgact aaagaaatta tcaaaatcaa cgcgaaagtt attttaccgt
    14521 cactcgccag gcgaaatgat cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa
    14581 cagccttcac aagcagtatt caatcaagaa ctctatcctg aaatctatga aaaagcggcg
    14641 attttagcaa ttaatttagc taaaaaacat ccttttcata atggcaataa acgaactgct
    14701 cttgttgcca tgttgttgtt tcttcaattg aataattgtc cagttgcttt ttctcgtcaa
    14761 gaagctgttg attttatcat aatgattacc actagttcat tggatttcga ttcactaaag
    14821 tccaagataa ccagttattt acgacaaacc gcaataaaat gaacgaatta aaaactttat
    14881 ttctcaatta gatcagcatg tcattaagga atatgctgat ttttttatgt ttaatcatac
    14941 aaaatagttt tttcataaaa atatggaaaa tatggattaa ttttgaaata attgatttaa
    15001 aaggaataca aaagagtgaa tagtaggaaa tagtggttta gaactagtgg gctgattttt
    15061 tatatatttt agtttacata atatatatta tacaaagtag atttaaaaaa tgataaacct
    15121 ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa
    15181 ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac
    15241 cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc
    15301 gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta
    15361 atcaacagat taaatttcat atgttcatat gtacggatac attttcggac cgattaattt
    15421 taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat
    15481 ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt
    15541 gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt
    15601 gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt
    15661 aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg
    15721 ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt
    15781 tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc
    15841 taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc
    15901 gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc
    15961 gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt
    16021 atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc
    16081 gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga
    16141 tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa
    16201 acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt
    16261 acattcttca tagccacctg accatacagc cacattttca tcgccccaag ccataccatc
    16321 taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc
    16381 attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc
    16441 gacgattact actttacgac ttgttttttt catgaataaa cagctccttt tcataactat
    16501 ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa
    16561 tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga
    16621 caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat
    16681 ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct
    16741 tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac
    16801 atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat
    16861 tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt
    16921 ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa
    16981 gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat
    17041 gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg
    17101 aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat
    17161 ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca
    17221 aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt
    17281 tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat
    17341 tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt
    17401 gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt
    17461 ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac atataattga
    17521 tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat
    17581 gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt
    17641 aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt
    17701 cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt
    17761 aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt
    17821 ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag
    17881 aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta
    17941 tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg
    18001 gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg
    18061 ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta
    18121 gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat
    18181 cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag
    18241 ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct
    18301 tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg
    18361 gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct
    18421 ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac
    18481 ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga
    18541 atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg
    18601 tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag
    18661 cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc
    18721 ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct
    18781 ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag
    18841 gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca
    18901 agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca
    18961 ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg
    19021 ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac
    19081 acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa
    19141 caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc
    19201 tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag
    19261 aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt
    19321 ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg
    19381 ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg
    19441 gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg
    19501 agggagacga agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc
    19561 cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc
    19621 aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt
    19681 ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta
    19741 tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata
    19801 agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta
    19861 taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt
    19921 cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag
    19981 aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct
    20041 ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg
    20101 aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata
    20161 tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga
    20221 tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat
    20281 acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat
    20341 ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac
    20401 tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga
    20461 acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat
    20521 ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa
    20581 gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc
    20641 aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc
    20701 catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct
    20761 accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg
    20821 aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg
    20881 gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta
    20941 tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt
    21001 catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa
    21061 caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta
    21121 aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg
    21181 cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt
    21241 ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct
    21301 ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac
    21361 tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata
    21421 atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa
    21481 taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct
    21541 accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac
    21601 cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac
    21661 aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg
    21721 aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg
    21781 aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa
    21841 tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc
    21901 aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat
    21961 gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt
    22021 agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt
    22081 tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca
    22141 aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca
    22201 caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag
    22261 cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt
    22321 cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat
    22381 cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat
    22441 cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa
    22501 tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac
    22561 gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa
    22621 caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca
    22681 agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga
    22741 gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat
    22801 cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat
    22861 ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta
    22921 atccactttg acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct
    22981 aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt
    23041 agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag
    23101 atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc
    23161 agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg
    23221 tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata
    23281 taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct
    23341 tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc
    23401 ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga
    23461 tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac
    23521 aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg
    23581 atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc
    23641 ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc
    23701 tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga
    23761 agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt
    23821 atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg
    23881 aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat
    23941 gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca
    24001 ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca
    24061 ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg
    24121 atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca
    24181 acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag
    24241 ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca
    24301 gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa
    24361 taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta
    24421 tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg
    24481 agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt
    24541 cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga
    24601 caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta
    24661 gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc
    24721 atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata
    24781 tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca
    24841 tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg
    24901 atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata actaattttg
    24961 tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga
    25021 ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat
    25081 cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc
    25141 gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac
    25201 atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc
    25261 ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt
    25321 tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc
    25381 cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc
    25441 ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca
    25501 gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt
    25561 cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt
    25621 tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt
    25681 aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca tctatatagg
    25741 tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa
    25801 acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc
    25861 cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt
    25921 tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa
    25981 gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta
    26041 ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt
    26101 ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt
    26161 ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt
    26221 aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg
    26281 acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc
    26341 catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat
    26401 cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt
    26461 cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca
    26521 aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg
    26581 cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc
    26641 tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt
    26701 tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata
    26761 gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc
    26821 acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg
    26881 acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc
    26941 aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat
    27001 caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat
    27061 agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca
    27121 caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag
    27181 cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa
    27241 atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat
    27301 gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat
    27361 cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc
    27421 ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag
    27481 gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta
    27541 aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa
    27601 aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag
    27661 atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa
    27721 aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata
    27781 gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa
    27841 aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa
    27901 atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa
    27961 cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga
    28021 gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg
    28081 tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat
    28141 cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc
    28201 tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg
    28261 aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga
    28321 tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg
    28381 caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt
    28441 gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga
    28501 ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc
    28561 taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat
    28621 ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat
    28681 aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac
    28741 taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact
    28801 cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt
    28861 ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg
    28921 atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc
    28981 agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta
    29041 aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc
    29101 gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt
    29161 gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca
    29221 tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa
    29281 aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta
    29341 ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt
    29401 tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa
    29461 gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt
    29521 ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg
    29581 acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt
    29641 gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta
    29701 tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga
    29761 gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat caactggcct
    29821 ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg
    29881 aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg
    29941 atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt
    30001 agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc attaccgact
    30061 gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca
    30121 tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac
    30181 actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta
    30241 ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca
    30301 ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc
    30361 gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat
    30421 tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc
    30481 attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg
    30541 ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt
    30601 tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa
    30661 agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg
    30721 gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg tccgcctctt
    30781 tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac
    30841 cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa
    30901 aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt
    30961 atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc
    31021 cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat
    31081 atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga
    31141 tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt
    31201 ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct
    31261 tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag
    31321 gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga
    31381 tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat
    31441 ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt
    31501 taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa
    31561 ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat
    31621 acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt
    31681 cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt
    31741 ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag
    31801 aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata
    31861 aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca
    31921 atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag
    31981 ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta
    32041 gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat
    32101 ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc
    32161 ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc
    32221 atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc
    32281 ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt
    32341 taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat
    32401 ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt
    32461 gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt
    32521 tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc
    32581 ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt
    32641 accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat
    32701 tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt
    32761 tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa
    32821 aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat
    32881 ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg
    32941 cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt
    33001 ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc
    33061 tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat
    33121 tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg
    33181 agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg
    33241 attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa
    33301 tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg
    33361 aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt
    33421 aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg
    33481 aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc
    33541 aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc
    33601 ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa
    33661 atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt
    33721 aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc
    33781 gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc
    33841 taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc
    33901 atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc
    33961 cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc
    34021 catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg
    34081 gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt
    34141 agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata
    34201 ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac
    34261 ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg
    34321 tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc
    34381 accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc
    34441 tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc
    34501 gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg
    34561 cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt
    34621 acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata
    34681 ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta taccatccca
    34741 ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg
    34801 ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg
    34861 attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga
    34921 attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc
    34981 tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag
    35041 cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg
    35101 tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc
    35161 atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc
    35221 tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga
    35281 ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc
    35341 tgctattgca gataatggca caaagtttga taaactcaat attatcagca tgaaaataac
    35401 tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga
    35461 cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat
    35521 gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt
    35581 catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg
    35641 agaaaaacat atgatcaggt tgttggaagg aaactataga aagatggaaa aaatcattcg
    35701 aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag
    35761 ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt
    35821 aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta
    35881 tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat
    35941 tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc
    36001 ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt
    36061 gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt
    36121 tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa
    36181 aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga
    36241 aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc
    36301 ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga
    36361 taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat
    36421 aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa
    36481 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
    36541 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
    36601 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
    36661 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
    36721 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
    36781 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
    36841 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
    36901 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta
    36961 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
    37021 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
    37081 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
    37141 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
    37201 actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
    37261 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
    37321 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
    37381 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
    37441 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
    37501 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
    37561 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
    37621 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
    37681 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
    37741 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
    37801 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
    37861 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
    37921 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
    37981 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
    38041 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
    38101 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
    38161 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
    38221 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
    38281 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
    38341 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
    38401 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
    38461 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
    38521 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
    38581 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
    38641 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
    38701 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
    38761 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
    38821 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
    38881 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
    38941 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
    39001 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
    39061 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
    39121 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
    39181 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
    39241 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
    39301 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
    39361 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
    39421 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
    39481 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
    39541 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
    39601 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
    39661 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
    39721 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
    39781 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
    39841 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
    39901 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
    39961 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
    40021 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
    40081 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
    40141 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
    40201 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
    40261 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
    40321 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
    40381 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
    40441 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
    40501 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
    40561 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
    40621 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
    40681 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
    40741 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
    40801 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
    40861 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
    40921 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
    40981 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
    41041 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
    41101 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
    41161 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
    41221 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
    41281 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
    41341 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
    41401 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
    41461 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
    41521 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
    41581 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
    41641 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
    41701 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
    41761 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
    41821 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
    41881 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
    41941 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
    42001 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
    42061 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
    42121 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
    42181 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
    42241 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
    42301 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
    42361 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
    42421 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
    42481 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
    42541 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
    42601 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
    42661 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
    42721 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
    42781 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
    42841 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
    42901 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctc
//
LOCUS       DASJTM010000014        40804 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJTM010000014 DASJTM010000000
VERSION     DASJTM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.014"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..920
                     /gene="guaC"
                     /locus_tag="U4B29_000714"
     CDS             <1..920
                     /gene="guaC"
                     /locus_tag="U4B29_000714"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN2105683.1"
                     /translation="RSECDTTVTLGKHTFKMPVVPANMQTIIDETIAEFLAENGYFYI
                     MHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIEKLAEESLNPDYITIDIAHGH
                     ANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKT
                     GFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEES
                     PGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQHKGSLKDTLVEMQQDLQSSI
                     SYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            1184..2608
                     /locus_tag="U4B29_000715"
     CDS             1184..2608
                     /locus_tag="U4B29_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2105684.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            3069..4178
                     /gene="pdhA"
                     /locus_tag="U4B29_000716"
     CDS             3069..4178
                     /gene="pdhA"
                     /locus_tag="U4B29_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN2105685.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            4181..5158
                     /locus_tag="U4B29_000717"
     CDS             4181..5158
                     /locus_tag="U4B29_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN2105686.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            5183..6826
                     /locus_tag="U4B29_000718"
     CDS             5183..6826
                     /locus_tag="U4B29_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN2105687.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            6834..8240
                     /gene="lpdA"
                     /locus_tag="U4B29_000719"
     CDS             6834..8240
                     /gene="lpdA"
                     /locus_tag="U4B29_000719"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN2105688.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            8402..8677
                     /locus_tag="U4B29_000720"
     CDS             8402..8677
                     /locus_tag="U4B29_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN2105689.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            8683..9450
                     /locus_tag="U4B29_000721"
     CDS             8683..9450
                     /locus_tag="U4B29_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN2105690.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            9618..11447
                     /gene="typA"
                     /locus_tag="U4B29_000722"
     CDS             9618..11447
                     /gene="typA"
                     /locus_tag="U4B29_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN2105691.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            11708..13480
                     /gene="recQ"
                     /locus_tag="U4B29_000723"
     CDS             11708..13480
                     /gene="recQ"
                     /locus_tag="U4B29_000723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN2105692.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            13594..13896
                     /locus_tag="U4B29_000724"
     CDS             13594..13896
                     /locus_tag="U4B29_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN2105693.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            13899..15062
                     /locus_tag="U4B29_000725"
     CDS             13899..15062
                     /locus_tag="U4B29_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2105694.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            15088..18516
                     /locus_tag="U4B29_000726"
     CDS             15088..18516
                     /locus_tag="U4B29_000726"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN2105695.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            18537..19661
                     /locus_tag="U4B29_000727"
     CDS             18537..19661
                     /locus_tag="U4B29_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105696.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            19651..20088
                     /locus_tag="U4B29_000728"
     CDS             19651..20088
                     /locus_tag="U4B29_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2105697.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            20072..20410
                     /locus_tag="U4B29_000729"
     CDS             20072..20410
                     /locus_tag="U4B29_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN2105698.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            20529..21089
                     /gene="rsmD"
                     /locus_tag="U4B29_000730"
     CDS             20529..21089
                     /gene="rsmD"
                     /locus_tag="U4B29_000730"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN2105699.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            21086..21577
                     /gene="coaD"
                     /locus_tag="U4B29_000731"
     CDS             21086..21577
                     /gene="coaD"
                     /locus_tag="U4B29_000731"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN2105700.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            21567..22610
                     /locus_tag="U4B29_000732"
     CDS             21567..22610
                     /locus_tag="U4B29_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN2105701.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            22769..23497
                     /locus_tag="U4B29_000733"
     CDS             22769..23497
                     /locus_tag="U4B29_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN2105702.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            23613..23858
                     /locus_tag="U4B29_000734"
     CDS             23613..23858
                     /locus_tag="U4B29_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2105703.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            23855..24073
                     /locus_tag="U4B29_000735"
     CDS             23855..24073
                     /locus_tag="U4B29_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN2105704.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            24209..24748
                     /locus_tag="U4B29_000736"
     CDS             24209..24748
                     /locus_tag="U4B29_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN2105705.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            24741..27014
                     /locus_tag="U4B29_000737"
     CDS             24741..27014
                     /locus_tag="U4B29_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN2105706.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            27052..28098
                     /gene="holA"
                     /locus_tag="U4B29_000738"
     CDS             27052..28098
                     /gene="holA"
                     /locus_tag="U4B29_000738"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN2105707.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(28187..28441)
                     /locus_tag="U4B29_000739"
     CDS             complement(28187..28441)
                     /locus_tag="U4B29_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN2105708.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(28841..29110)
                     /locus_tag="U4B29_000740"
     CDS             complement(28841..29110)
                     /locus_tag="U4B29_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105709.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(29580..30035)
                     /locus_tag="U4B29_000741"
     CDS             complement(29580..30035)
                     /locus_tag="U4B29_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN2105710.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(30173..32101)
                     /locus_tag="U4B29_000742"
     CDS             complement(30173..32101)
                     /locus_tag="U4B29_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2105711.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(32298..33131)
                     /locus_tag="U4B29_000743"
     CDS             complement(32298..33131)
                     /locus_tag="U4B29_000743"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN2105712.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(33323..34027)
                     /locus_tag="U4B29_000744"
     CDS             complement(33323..34027)
                     /locus_tag="U4B29_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2105713.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(34050..34676)
                     /locus_tag="U4B29_000745"
     CDS             complement(34050..34676)
                     /locus_tag="U4B29_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2105714.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            35002..37377
                     /locus_tag="U4B29_000746"
     CDS             35002..37377
                     /locus_tag="U4B29_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN2105715.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(37401..37892)
                     /locus_tag="U4B29_000747"
     CDS             complement(37401..37892)
                     /locus_tag="U4B29_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN2105716.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            38079..38999
                     /locus_tag="U4B29_000748"
     CDS             38079..38999
                     /locus_tag="U4B29_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2105717.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            39153..39497
                     /locus_tag="U4B29_000749"
     CDS             39153..39497
                     /locus_tag="U4B29_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN2105718.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(39687..40187)
                     /locus_tag="U4B29_000750"
     CDS             complement(39687..40187)
                     /locus_tag="U4B29_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105719.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            40715..40798
                     /locus_tag="U4B29_000751"
     tRNA            40715..40798
                     /locus_tag="U4B29_000751"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40749..40751,aa:Leu,seq:cag)
BASE COUNT        13568 a         7120 c         8477 g        11639 t
ORIGIN      
        1 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
       61 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
      121 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
      181 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
      241 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
      301 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
      361 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
      421 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
      481 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
      541 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
      601 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
      661 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
      721 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
      781 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
      841 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
      901 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
      961 tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg
     1021 aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa
     1081 gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc
     1141 ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga
     1201 gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa
     1261 cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg
     1321 tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac
     1381 gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc
     1441 aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca
     1501 aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga
     1561 gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt
     1621 agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg
     1681 gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg
     1741 cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc
     1801 tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt
     1861 atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca
     1921 taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt
     1981 gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat
     2041 tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat
     2101 acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa
     2161 aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc
     2221 gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt
     2281 taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg
     2341 actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg
     2401 ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt
     2461 agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat
     2521 cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa
     2581 aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac
     2641 agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga
     2701 aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt
     2761 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
     2821 agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac
     2881 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
     2941 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
     3001 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
     3061 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
     3121 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
     3181 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
     3241 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
     3301 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
     3361 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
     3421 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
     3481 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
     3541 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
     3601 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
     3661 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
     3721 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
     3781 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
     3841 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
     3901 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
     3961 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
     4021 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
     4081 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
     4141 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
     4201 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
     4261 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
     4321 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
     4381 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
     4441 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
     4501 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
     4561 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
     4621 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
     4681 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
     4741 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
     4801 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
     4861 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
     4921 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
     4981 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
     5041 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
     5101 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
     5161 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg
     5221 aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga
     5281 tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac
     5341 aggaacggtt aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt
     5401 agtagaaatc gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga
     5461 atcccaaact ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc
     5521 gtcaagcgaa ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg
     5581 tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact
     5641 agaagttcaa aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa
     5701 aaacgtgatt gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga
     5761 tgctcctgga cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga
     5821 aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt
     5881 attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt
     5941 aactgcaact ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg
     6001 tggtccaagc agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc
     6061 agctcctgca gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg
     6121 agacttggaa gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt
     6181 caacagtaag catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact
     6241 atgggataat cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt
     6301 cttgccatat gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc
     6361 atctatcgac gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc
     6421 aactgatacg gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat
     6481 gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc
     6541 tgcagaagat atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg
     6601 ttggtttaca ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc
     6661 acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc
     6721 attaagtttc gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat
     6781 caaacgtctt ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag
     6841 ttggagattt tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg
     6901 tagctgccat ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata
     6961 ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac
     7021 accattatca agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac
     7081 tagattttgc aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg
     7141 gtgttggttt cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg
     7201 tagatgatca cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata
     7261 acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac
     7321 gtgtgttaga ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca
     7381 tcggtggcgg tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag
     7441 taacgattct tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat
     7501 tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag
     7561 aagcagtcga taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat
     7621 ccgttactgc tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg
     7681 gcttagaaca agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag
     7741 gacgtacgaa cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag
     7801 cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag
     7861 ctgtagatta taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag
     7921 gtatgacgat caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat
     7981 tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta
     8041 cgacgatcga agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata
     8101 tggtctctga attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga
     8161 cgattcatcc tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag
     8221 gtttaccaat ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg
     8281 cgttttgttt tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt
     8341 cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa
     8401 gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt
     8461 aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt
     8521 tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt
     8581 gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa
     8641 aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat
     8701 catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta
     8761 acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag
     8821 aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa
     8881 gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat
     8941 ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa
     9001 tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt
     9061 ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc
     9121 ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc
     9181 cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta
     9241 gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat
     9301 gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact
     9361 cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa
     9421 atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga
     9481 taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg
     9541 aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa
     9601 aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca attattgccc
     9661 acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag
     9721 acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg
     9781 gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca
     9841 tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg
     9901 acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat
     9961 tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac
    10021 cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag
    10081 cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa
    10141 gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta
    10201 ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat
    10261 tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa
    10321 tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg
    10381 taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg
    10441 gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg
    10501 tggatcatca agatgcgtta cctatccttc acattgacga accaacattg caaatgactt
    10561 tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga
    10621 ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa
    10681 actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg
    10741 aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag
    10801 aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat
    10861 atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc
    10921 atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt
    10981 attcaactga attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc
    11041 aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga
    11101 tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct
    11161 tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata
    11221 atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta
    11281 aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat
    11341 tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa
    11401 ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat
    11461 gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt
    11521 ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt
    11581 aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc
    11641 acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag
    11701 cgggaagatg aacgacttat taaaacaata ctttggctat gatgagttta gacctggtca
    11761 aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg
    11821 aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt
    11881 tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat
    11941 acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga
    12001 aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga
    12061 ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg
    12121 tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga accagctaga
    12181 tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc
    12241 tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga
    12301 acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga
    12361 atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt
    12421 agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg
    12481 tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct
    12541 gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat
    12601 ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag
    12661 agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa
    12721 gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt
    12781 aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta
    12841 ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat
    12901 agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa
    12961 ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc
    13021 tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac
    13081 gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta
    13141 tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta
    13201 tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga
    13261 agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt
    13321 attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat
    13381 gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc
    13441 tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa
    13501 tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta
    13561 taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg
    13621 atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac
    13681 agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc
    13741 ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag
    13801 ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa
    13861 atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa
    13921 ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga
    13981 tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat
    14041 cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata
    14101 aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga
    14161 cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc
    14221 tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt
    14281 acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg
    14341 tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt
    14401 ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca
    14461 attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct
    14521 ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc
    14581 gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca
    14641 acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc
    14701 agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag
    14761 aactgggagt gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca
    14821 ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg
    14881 gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt
    14941 tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga
    15001 tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat
    15061 aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa
    15121 atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat
    15181 gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga
    15241 caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa
    15301 gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt
    15361 gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat
    15421 atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt
    15481 ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac
    15541 ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct
    15601 cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc
    15661 tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta
    15721 cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt
    15781 cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa
    15841 cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca
    15901 ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc
    15961 gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa
    16021 ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga
    16081 attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac
    16141 tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt
    16201 ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta
    16261 gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc
    16321 ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc
    16381 acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca
    16441 ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa
    16501 attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca
    16561 cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat
    16621 gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg
    16681 gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat
    16741 ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg
    16801 tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg
    16861 ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca
    16921 aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca
    16981 gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg
    17041 gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat
    17101 actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg
    17161 gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg
    17221 cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca
    17281 atccatctgc atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca
    17341 aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag
    17401 cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg
    17461 attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg
    17521 tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga
    17581 cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa
    17641 atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca
    17701 tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa
    17761 gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa
    17821 ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa
    17881 ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa
    17941 gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc
    18001 tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat
    18061 gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat
    18121 gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat
    18181 atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc
    18241 aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga
    18301 gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat
    18361 aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc
    18421 gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg
    18481 tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga
    18541 aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac
    18601 cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc
    18661 aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa
    18721 aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat
    18781 ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca
    18841 tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc
    18901 cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt
    18961 tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt
    19021 atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa
    19081 caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat
    19141 atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat
    19201 caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag
    19261 acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac
    19321 atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa
    19381 aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga
    19441 agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg
    19501 acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg
    19561 acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc
    19621 aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg
    19681 gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat
    19741 cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc
    19801 ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat
    19861 cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt
    19921 gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta
    19981 cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg
    20041 aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat
    20101 aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa
    20161 aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa
    20221 tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt
    20281 agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac
    20341 agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga
    20401 acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg
    20461 atttttgatc attgtccaag tatttttttg gtatactagt accagtacca tttctaagga
    20521 gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag
    20581 gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat atgattggac
    20641 catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg
    20701 aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa
    20761 aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac
    20821 ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt
    20881 ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac
    20941 tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc
    21001 cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa
    21061 ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc
    21121 ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt
    21181 aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac
    21241 attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca
    21301 attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag
    21361 tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga
    21421 attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt
    21481 aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga
    21541 agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga
    21601 ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag
    21661 aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata
    21721 aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag
    21781 ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg
    21841 gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg
    21901 cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg
    21961 tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata
    22021 cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa
    22081 aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa
    22141 cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc
    22201 ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg
    22261 gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa
    22321 acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg
    22381 gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt
    22441 tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa
    22501 ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac
    22561 cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa
    22621 ttaaataggg gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca
    22681 cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg
    22741 ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc
    22801 attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa
    22861 attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt
    22921 atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc
    22981 gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg
    23041 tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt
    23101 ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc
    23161 ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc
    23221 tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca
    23281 gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact
    23341 tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga
    23401 aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac
    23461 agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac
    23521 gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga
    23581 cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa
    23641 aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg
    23701 ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct
    23761 atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac
    23821 tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta
    23881 tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata
    23941 tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca
    24001 tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt
    24061 tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca
    24121 gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc
    24181 aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac
    24241 gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt
    24301 tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc
    24361 gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga
    24421 tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca
    24481 tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga
    24541 aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt
    24601 gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga
    24661 acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca
    24721 aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga
    24781 tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt
    24841 atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg
    24901 cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt
    24961 catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg
    25021 caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc
    25081 tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg
    25141 gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata
    25201 attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa
    25261 agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat
    25321 cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt
    25381 tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg
    25441 gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt
    25501 attatttgtt tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac
    25561 taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc
    25621 tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat
    25681 ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg
    25741 gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt
    25801 tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc
    25861 atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca
    25921 agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa
    25981 ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt
    26041 cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg
    26101 gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg
    26161 gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt
    26221 ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga
    26281 ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga
    26341 tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa
    26401 tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat
    26461 ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat
    26521 tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg
    26581 gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg
    26641 gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt
    26701 taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag
    26761 ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt
    26821 cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc
    26881 cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc
    26941 aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa
    27001 caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt
    27061 aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac
    27121 ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa
    27181 atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt
    27241 ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta
    27301 ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat
    27361 actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt
    27421 gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca
    27481 attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc
    27541 ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc
    27601 gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat
    27661 caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat
    27721 atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac
    27781 gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa
    27841 ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt
    27901 gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa
    27961 ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc
    28021 aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag
    28081 ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt
    28141 ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt
    28201 ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt
    28261 cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg
    28321 aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat
    28381 ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca
    28441 ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat
    28501 tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca
    28561 gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa
    28621 agacgctatt gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa
    28681 acaaagtttg aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc
    28741 aaagtttttt ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac
    28801 tgcagttatt ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta
    28861 ttttaatgta ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac
    28921 ggttccattt gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt
    28981 ttgccaaata gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga
    29041 tcccagtcag catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa
    29101 aagtttccac aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt
    29161 ccattttgat attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag
    29221 tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt
    29281 ctcattggca cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag
    29341 gagcatcccc tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta
    29401 tcaaatcagg aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc
    29461 agccgattgt tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt
    29521 atatcacgga tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc
    29581 tattttccta aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt
    29641 ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca
    29701 gtgatttgtg gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc
    29761 ccaccgcaaa aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt
    29821 agaccgattt ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca
    29881 atattgattc ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc
    29941 ataatcaaaa caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca
    30001 agtaaaataa ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga
    30061 aacaatcata acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat
    30121 tcataagctg tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga
    30181 cagaagacag ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa
    30241 gatcaggtaa aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt
    30301 cgtgtcctac aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca
    30361 agagaataac aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag
    30421 atagccgatg actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga
    30481 tatccatagc ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag
    30541 caggcgcatc attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt
    30601 ttttgactag ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta
    30661 cttgttcagc aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga
    30721 tgttttggga tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca
    30781 gacctaaaac agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat
    30841 tttccaaacg ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat
    30901 atgcgtgctt gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag
    30961 taatttcttc tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca
    31021 aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg
    31081 ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg
    31141 ttcctgtttt atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc
    31201 ctttgattaa aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag
    31261 cagatgcgac aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc
    31321 tttcatccca actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa
    31381 gcaaaatcaa ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg
    31441 gcgtattctg cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg
    31501 tttttgttac tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat
    31561 cgcctaaata tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg
    31621 cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca
    31681 agttatccac ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata
    31741 attgggcttt ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag
    31801 ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa
    31861 agtaattacc gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca
    31921 gtgtccgatc attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga
    31981 taatggcact gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac
    32041 cgaagatcat aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca
    32101 tcgttattag acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa
    32161 taacagctaa atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt
    32221 attatttata atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag
    32281 aagtgttctg gttttttcta cattgcaata aaccaataac caatcaaaat gataccaagg
    32341 ataatacgat accagccaaa agcggtaaag tcgtttttct tcaaatagct tagtaagaat
    32401 ttgatagcta agacggaaac cacaaaagat acgaatgttc ctacaagaag aacaacagtt
    32461 tcttccattt tgaatgaatt cccttcagct aaatatttga cgatcttcaa gccgcttgcg
    32521 ccaaacatga caggaatccc taagaaaaat gaaaactctg tagcgacaaa acgcgaagca
    32581 ccaatcaaga tagatcctag gatcgttgcg ccagaacggg atgttccggg aacaagtgcc
    32641 aagacttgga ataacccaac aagaagagct gcttggtacg taaagtcatt cagagaagtc
    32701 caacgaggtg ttttgttttt gttgcgtttt tcgacaataa taaaggcaat accgtaaacg
    32761 atcaaaacaa tggcaactgg taagaacttg tggaaatttg cttctaacca atcatccaaa
    32821 ggcaaaccaa tgatgatggc tggtatgcaa gcaacgatca ctttaaacca aagtgtccac
    32881 gtatcttttt tttctaccgt tgatttactt ggagaaaatg ggtttaactt gtgaaaataa
    32941 agaacgacga cagccaagat cgcccctaat tggataacga cattgaacat cgacataaat
    33001 tgtgaactgg catccagttt gatgaattca tctgctagga tcaaatgacc agtactactg
    33061 atgggcagcc attccgttat tccttcaata ataccgagaa tcactgcttt gataatattt
    33121 gcaaaaaaca taaattcctt ccttcccaat taagatacat cttatagtat accgttattt
    33181 tttgagaaac ccaatctgtt ttttaatatt aagaaaaact tgagaaataa agcagataag
    33241 tacacgaaga gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt atgattctct
    33301 ggtaaaaatt aaaatatccc cattatagaa acgagtcgcg actatgtgtt ttcccgttat
    33361 cgatataaga cgtatcattg atgccgtgga tagaaaatcg accgtttgca taagtgacct
    33421 ttgttacgct gccatttaaa agcccagcac gaacaggctg tttaggatcg atcagattaa
    33481 gcaaaaaggc gatcgtcaaa ccatgagata cgactaaaac atttccacca ccattttttt
    33541 cgcagtgatg ggcgatatct tcaaatcctg tccatacacg gtctcgcaat gtttcataag
    33601 tttccgccca cccagcggta tctaagttgt atacagcatt agcaatttgt tcaaaagaaa
    33661 cttctgtggt aaacatttca tcatatgttt taaaattcaa tacacgaggg atcacacccc
    33721 acatttccat gtcatatcca ccttcaagag acccgaaaca ccattctcta atcctttttt
    33781 caataaaata aggaatttct tttcccttag aatgttctcc tagaataata cgggcagttt
    33841 cgattgcacg accactgtca ctagaataag caaatgaaaa gtcgacattt tttaacccgc
    33901 gacctaaata atgaatgcct tcttgtcctt gctttgtgag gggtgtatca caccagcctt
    33961 gtactcgttc aattgtattg aacatcgttt ttccgtgtcg tacgatataa agtgtagttt
    34021 ctgtcatttt attctcctcc taagtcgttt taacgattga aaaaaggatt tgtccttttt
    34081 tcctgttcaa tcgtcgttgc atcgccatgt cctggataga ctgcaaattc atcaggtaat
    34141 acaaacagct gggtccgaat actatgaagt aattgttcta aattccctgt atacagatcg
    34201 gttctaccga tacttcctct aaacagggcg tcgccagaaa cgacaaaatc atcaaaaata
    34261 aagctgacgc ttcctataga atgccctggg gtagggacga cgcgaaaaga catgtttcct
    34321 aaagtataag ttttcatttc aaattcatat tctgcaggcg agacaataac atcagcaata
    34381 tcatcatgtc taccgagtcc agaaagattc aaaatcggat cacctagcca tttttgctct
    34441 agtgggctga cataaactgg gatttgataa tacgcgcgta aaggttccac tgcaccgata
    34501 tgatcgtagt gagtatgggt aagcaaaatc gctaccggct gttggtttgt ttttttgatt
    34561 tcttgtttga ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc ttcttcgttg
    34621 tagaccaaat aacagttttc ttcaatcatt cctgttttta atctttcgat atgcatgtgc
    34681 attcctcctt gatagccaaa acttctttca tccattagta taacgcattc attgctagga
    34741 aacaaaaaca ggtacttatt tctctttact tatctgaatt atcagactat atgaaaagta
    34801 tttggatatt ctaaagagaa ggaaagtaag ttttcatagc attttaatat gtccttgtta
    34861 cattagattc atccaaaaat ccaaattcta tatcctatat ccctgcctaa tcagcagggt
    34921 gtttttttat gtttggaaag agagctcttt ttgctaaact aggttttttc aggtaatctt
    34981 agagagaaag aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg gcacttagta
    35041 gaatttattt tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca tactgcatta
    35101 gaaggagcga gaatccaccg caagctccaa aaagatgcgg gagaaaacta tgaaaaagaa
    35161 gtctttttaa aaacaacagt tcaattagat gaatattacc tgatcgttga aggccgtgca
    35221 gacggtattt tcaaaagaga cggtatttat tatatagatg aaatcaaaac ctccgaacca
    35281 cgatttgaag atttagaacc agaacaagtg gaacttttct ttcatcaagc aagggtgtat
    35341 gcctatatct atagtcaaga acatgattta caagaaataa atctgcaatt gacttacttc
    35401 cagacggcag aagaagtgat cacgagaaag gttgaacatc agacaagaga gcagttagaa
    35461 acttttttta aaaagctgac tgaagattat aaagaatggc ttgtttttca agaaaactgg
    35521 cgcactgtac gcaatgcctc tttgatggct ttgaaattcc cccatgatga gtataggaaa
    35581 ggtcagcgtg aactagcggt agctgcttat aaaacgatcc gtacgaaaca gaaattgttt
    35641 gtagaagcac ctaccggaac aggaaaaaca atctctacgt tattccccgc tttgaaagct
    35701 gtaggggaag aagagggaga aaagatcttt tatctaacgg ctaaaacaat aactagacaa
    35761 gtagctgaag atgctatgac agcattaaaa gatacaggag ctgaagtaaa aagtgtcact
    35821 ttgacggcga aagacaagat ctgcttttta gatgagacga cctgtaatcc agatcagtgc
    35881 ccatatgcca atggttatta caatcggatt aatgaaggtt tatgggatct tttgaatcat
    35941 gagaatcaga tcactcgtga agtcatcgaa acttatgcaa gaaagcatac actttgtcct
    36001 ttcgaactat cattagatgt cagtatctgg tgtgacgtaa tcattgggga ttacaattat
    36061 ttatttgatc caactgttta tttaagacgt ttttttgaag atgaaaaaaa cgaggactat
    36121 ctgtttttga ttgacgaagc acataatttg gtgaatcgtt cccgagagat gtattcggca
    36181 gaactatctt atgaaaaaac gaaacgaagt aaggaagcta taccaaagga attcaaaaaa
    36241 cttcatcgcc gtttcaacaa actattaaaa gaatttgatt cgatccgaga aatagcaaaa
    36301 gaagatcatt gggattatca tcatcaaaaa gcaccggctg aatctttagt aaaggcaggt
    36361 tatcaattaa gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca cccatcacaa
    36421 gaacaattgc ttccttatta ttttgatttg cttcattttt tgaaggtcag tgaattttat
    36481 gatgatcact acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt aaaagagttt
    36541 tgtattgatc caagcttgtt tcttgagcag tcgttggata aaggaaaaag cagtattctt
    36601 ttttctgcta gtttttcacc actctcttat tatcaggaaa cactgggggg acaaaaaagt
    36661 ctggcttaca aactgccgag tcctttccca gaaggaaacc agcaagtttt catcgcaaat
    36721 tacctagaga cgacttatcg aaaaagggaa cacagtctgg caaaattagt agagacgatt
    36781 catgcttttg ctgatggaaa ggtcgggaat tatttcgttt tctttccttc ttatcaatat
    36841 ttagatcttg cggtggaagc attcagacag caatatcccg acagcaatgt gctaatccaa
    36901 gaaacggaca tgaatgagga agaacgcgaa cgatttttaa caaaatttca aacaaatccc
    36961 gctgaaacat tgattggatt ttgcgtactt ggcgggattt tttcagaagg aatcgatttg
    37021 agaggggacc gattgatcgg cagtatgatc gtaggtgtag gtttgccgca gatgaatcat
    37081 gagcaggaac tgatcaaatc atattatgat gagaaggaac accttggatt tgcttacgct
    37141 tatcagcttc ctggaatgaa caaagtcttg caagctggtg gtcgagtgat tcgtggcatg
    37201 gcggatcaag gaatcgttgt attggcagat caacgatttt ctagtttttc ttatcgacgt
    37261 ttattcccac aacattggca aacagcaaga gaagtacgaa gtgttgggga attggaagaa
    37321 ggaatacagc gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga ggaatgacag
    37381 acgtcagcct cgttttttga ttaacgatag ttagtgaatt gcaactcgat tggtaagtca
    37441 agtttacgta ataaagcaat cacagcttgt agatcatcac ggttcttacc tgtcactcta
    37501 atctgatctt cttggatttg tgcttttact ttcagttttt catttttgat agctgtagtg
    37561 attttcttcg cgttttcacg atcgattccg ttgaccaaat cggcagtttg tcgagctttg
    37621 ccacctagag cgtgttctga ttcggaaaaa tggatatttt tgattggcac atttcttttg
    37681 atcagcttac caaataatac atctttgatc tgttcgattt tgaaatcatc atcgctcaat
    37741 acaactagat tgctgttctc taatttgatt tctactgtag aatccttgaa gtcaaagcga
    37801 ttggacaatt cttttgttgt ttgctgaatc gcatttttta cttcttctgt attgacttct
    37861 gatacaatgt cgaagctagc atcttttgcc ataggaaccc tcctgatata catataaata
    37921 tttaccatac atctatctta tcataggatt agggcagatt cttacctgtt tgctaaaatt
    37981 cttctcattt gagttcgaag tattttattt taatagataa actgttattc ttacatttgg
    38041 aataccaaag taggggagag gtgcttgtga gtaaaattat gactgcgtat cataaaaatg
    38101 aaagattaaa gaaaatagtg gttattttga taaccggatt gaccgctgcg gtcgctttaa
    38161 attatttttt gattccagcc aatgtctttt ctgcagggat gaatgggatc gctcaaatca
    38221 tttcctcgct tttatatgaa tggtttcata tcaagataga cacaggaaca tttatttttc
    38281 ttttgaatat ccctgtgttt gtattagggt ttctcaaagt aggaaaacga gcaacgatcc
    38341 ttagttttgt gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca gtcggacaga
    38401 tcactgataa tattttgatg aatgccctag taggcggtgt tttattaggt cttggcgtag
    38461 gattatcttt aaaaatgggc tttacaactg gtgggatgga tatcatctct cttgtacttt
    38521 cacaaacaac tggaaaaaca gtcggaaact acatgctgat tctaaatggt atcattgtag
    38581 tcatcgcagg cttcttcttt aactgggaaa gtgccttgta tacgattatt tccatttatg
    38641 caatgactca agtaattgat gccatccaca cgagtcatca aaaagtaaca gcaatgatcg
    38701 ttaccgtcaa gccagaagag gtaacagaag aactagcaaa acggatggta cgtggaatga
    38761 cattactgcc gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc atgatggtca
    38821 tcacccgcta cgaaatgtat gatcttgatc agatcgttca tgaaatcgac gacgatgcat
    38881 ttatcaatat tttaccaact cattcagtat ttggccggtt tgcaaatgaa aacgaacaaa
    38941 aaatgtatcg aacaactggc gtattgccag aaataaaaaa tcgttcacaa aaaaaatgag
    39001 gaatcaggtt tattttagaa taaatttaat ctattacatc taataagaac atttttataa
    39061 atttaaacga gaatcttact tctaaattct catttacaag cagctaaaaa aagagtatga
    39121 taaataaaaa tcgtgttaag gagtgtcgta cgatgagcga agcaaaccaa gaacggtctg
    39181 gacaagaaat agaactaatc aaagaacgta ttcttgctgc cttggaaatg gtgattgatc
    39241 ctgaattagg aatcgacatc gtcaatttag gcttgattta tgatatagaa ttcaatcctg
    39301 aaaatggaga aacagtcatt aagatgactc taacaactat gggctgtcca ttagcagata
    39361 ttttaacaga atctatccat gacgcattaa aagaagtgcc agaagtaacg aaagcagaag
    39421 tcaaactagt ctggtatcca gcatggacga cagataagat gagccgttat gcacggattg
    39481 cgttggggat acgatagaaa tcttggctga ctagctatga gaagcaaata ggaaaaacaa
    39541 aaaagccttt tatcctttct agaagaaaag agataaaagg tttctttgtt tataaaattt
    39601 tgtctgctat attataagtt aattaaataa atgtgttcta ttgataaagg acattcaaat
    39661 ccctttcctt ttggaatcct tcttttttac atcagtttga ttttcgtata gtattctttc
    39721 tcatcttcac gataaatcgc agccccattt ttttgttgtg ctttaagact gccaagattt
    39781 tttttattta cagacaaaag tgcctcatca atgccgattt tttttgcctc ttgtagtact
    39841 aatgctaatc cttgtgtggc atatcctttt ttacgataat taggggagat gccgtaacca
    39901 atatggccag cgccgtttct taatgtttcg ttcagtttat ggcgcaaatt aaaaattcct
    39961 acatactcat gttcatcact ttctaaaata aagacggttg cgggaacgta actctcagga
    40021 agatcgattc ctaaagacgt tttttcgcat ttttttacat aatctaaaaa ttcagcaaat
    40081 gtatagccat aagcattgtt gataaatcca ctctcatttg gtccaaagct agtttgtagc
    40141 tgataagctt tctttaaatc tgcttcccat agtttgatca gtttcatcgt attcacctct
    40201 ttaagtaacc ttattcattt gttttctact atagcactat tgttgtaaat agccatgcct
    40261 atttttattt attgtgtcat atcgtattaa gaatcatcga acaatacaaa tatgaattac
    40321 aataaaaaaa ttatttgaag ttcgttcatt cgttacttat gatctctaga agaaagaaaa
    40381 cgtgaattac ttcagcgtct agaattccag aaggatagta atcaacagaa gaaataatca
    40441 atatccaaat gaaagaagat agctgaatcg agaaaaagag gaaaaaagaa atgaagaaaa
    40501 aaataaaata gttactaatc ccagagtcgt tcgtttaaca ttgaaatcga tcatatggat
    40561 aaaacagatt ttttcaccaa ccctcaatag atgatggagt tgctcggatg aattatttgt
    40621 gtagatggca taagggaggg cgtacagaaa tgaaaaagaa caaaaatatg ttcttgacaa
    40681 aaaacacatg taatcctaaa ctaaaagatg tgatgcggct atggcggaat tggcagacgc
    40741 gctagcttca ggcgctagtg agggtgacct cgtggaagtt cgagtcttct tagccgcata
    40801 attt
//
LOCUS       DASJTM010000015        39150 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJTM010000015 DASJTM010000000
VERSION     DASJTM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.015"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..328)
                     /locus_tag="U4B29_000752"
     CDS             complement(26..328)
                     /locus_tag="U4B29_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2105720.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="U4B29_000753"
     CDS             complement(353..2446)
                     /locus_tag="U4B29_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2105721.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="U4B29_000754"
     CDS             complement(2935..3699)
                     /locus_tag="U4B29_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN2105722.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U4B29_000755"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U4B29_000755"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN2105723.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="U4B29_000756"
     CDS             complement(4704..5780)
                     /locus_tag="U4B29_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN2105724.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="U4B29_000757"
     CDS             complement(5804..6397)
                     /locus_tag="U4B29_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN2105725.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U4B29_000758"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U4B29_000758"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN2105726.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="U4B29_000759"
     CDS             complement(8067..8366)
                     /locus_tag="U4B29_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN2105727.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U4B29_000760"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U4B29_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN2105728.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="U4B29_000761"
     CDS             complement(10121..11032)
                     /locus_tag="U4B29_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2105729.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="U4B29_000762"
     CDS             11152..12009
                     /locus_tag="U4B29_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2105730.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="U4B29_000763"
     CDS             complement(12196..12558)
                     /locus_tag="U4B29_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN2105731.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="U4B29_000764"
     CDS             12904..14343
                     /locus_tag="U4B29_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105732.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="U4B29_000765"
     CDS             complement(14350..16902)
                     /locus_tag="U4B29_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN2105733.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="U4B29_000766"
     CDS             complement(17016..18347)
                     /locus_tag="U4B29_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2105734.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="U4B29_000767"
     CDS             complement(18313..19791)
                     /locus_tag="U4B29_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2105735.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U4B29_000768"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U4B29_000768"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2105736.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="U4B29_000769"
     CDS             22401..23828
                     /locus_tag="U4B29_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105737.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="U4B29_000770"
     CDS             complement(23920..24285)
                     /locus_tag="U4B29_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2105738.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="U4B29_000771"
     CDS             complement(24282..24647)
                     /locus_tag="U4B29_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2105739.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="U4B29_000772"
     CDS             complement(24839..25087)
                     /locus_tag="U4B29_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105740.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="U4B29_000773"
     CDS             25086..25214
                     /locus_tag="U4B29_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN2105741.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="U4B29_000774"
     CDS             25271..26965
                     /locus_tag="U4B29_000774"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN2105742.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="U4B29_000775"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="U4B29_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27065..27238
                     /locus_tag="U4B29_000776"
     CDS             27065..27238
                     /locus_tag="U4B29_000776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105743.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="U4B29_000777"
     CDS             27616..28749
                     /locus_tag="U4B29_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN2105744.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="U4B29_000778"
     CDS             28780..29100
                     /locus_tag="U4B29_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN2105745.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="U4B29_000779"
     CDS             complement(29129..29593)
                     /locus_tag="U4B29_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105746.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..30978)
                     /locus_tag="U4B29_000780"
     CDS             complement(29725..30978)
                     /locus_tag="U4B29_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2105747.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31007..31324)
                     /locus_tag="U4B29_000781"
     CDS             complement(31007..31324)
                     /locus_tag="U4B29_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2105748.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31350..31670)
                     /locus_tag="U4B29_000782"
     CDS             complement(31350..31670)
                     /locus_tag="U4B29_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2105749.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32134..33549)
                     /locus_tag="U4B29_000783"
     CDS             complement(32134..33549)
                     /locus_tag="U4B29_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2105750.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33566..34882)
                     /locus_tag="U4B29_000784"
     CDS             complement(33566..34882)
                     /locus_tag="U4B29_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2105751.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35032..35730)
                     /locus_tag="U4B29_000785"
     CDS             complement(35032..35730)
                     /locus_tag="U4B29_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN2105752.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36019..36975)
                     /locus_tag="U4B29_000786"
     CDS             complement(36019..36975)
                     /locus_tag="U4B29_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2105753.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="U4B29_000787"
     CDS             complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="U4B29_000787"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2105754.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38096..>39150)
                     /locus_tag="U4B29_000788"
     CDS             complement(38096..>39150)
                     /locus_tag="U4B29_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105755.1"
                     /translation="STALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAF
                     WYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLI
                     LFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLIL
                     TMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAY
                     TTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQV
                     ITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVT
                     LTAYLCLFFKIVFHKR"
BASE COUNT        11807 a         8111 c         6646 g        12586 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
    30001 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
    30061 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
    30121 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
    30181 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
    30241 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
    30301 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
    30361 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
    30421 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
    30481 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
    30541 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
    30601 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
    30661 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
    30721 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
    30781 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
    30841 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
    30901 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
    30961 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
    31021 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
    31081 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
    31141 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
    31201 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
    31261 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
    31321 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
    31381 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
    31441 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
    31501 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
    31561 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
    31621 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
    31681 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
    31741 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
    31801 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
    31861 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
    31921 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
    31981 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
    32041 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
    32101 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
    32161 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
    32221 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
    32281 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
    32341 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    32401 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    32461 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    32521 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    32581 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    32641 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    32701 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    32761 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    32821 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    32881 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    32941 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    33001 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    33061 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    33121 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    33181 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    33241 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    33301 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    33361 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    33421 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    33481 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    33541 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    33601 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    33661 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    33721 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    33781 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    33841 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    33901 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    33961 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    34021 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    34081 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    34141 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    34201 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    34261 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    34321 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    34381 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    34441 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    34501 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    34561 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    34621 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    34681 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    34741 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    34801 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    34861 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    34921 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    34981 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    35041 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    35101 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    35161 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    35221 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    35281 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    35341 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    35401 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    35461 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    35521 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    35581 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    35641 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    35701 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    35761 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    35821 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    35881 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    35941 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    36001 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    36061 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    36121 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    36181 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    36241 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    36301 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    36361 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    36421 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    36481 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    36541 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    36601 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    36661 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    36721 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    36781 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    36841 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    36901 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    36961 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    37021 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    37081 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    37141 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    37201 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    37261 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    37321 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    37381 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    37441 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    37501 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    37561 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    37621 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    37681 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    37741 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    37801 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    37861 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    37921 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    37981 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    38041 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    38101 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    38161 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    38221 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    38281 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    38341 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    38401 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    38461 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    38521 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    38581 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    38641 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    38701 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    38761 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    38821 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    38881 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    38941 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    39001 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    39061 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    39121 aaataaaaat gataattaat gctgtactca
//
LOCUS       DASJTM010000016        38889 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJTM010000016 DASJTM010000000
VERSION     DASJTM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.016"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1752)
                     /gene="ade"
                     /locus_tag="U4B29_000789"
     CDS             complement(<1..1752)
                     /gene="ade"
                     /locus_tag="U4B29_000789"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN2105756.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            1884..2813
                     /gene="tadA"
                     /locus_tag="U4B29_000790"
     CDS             1884..2813
                     /gene="tadA"
                     /locus_tag="U4B29_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN2105757.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            2770..3819
                     /locus_tag="U4B29_000791"
     CDS             2770..3819
                     /locus_tag="U4B29_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN2105758.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            3816..4094
                     /locus_tag="U4B29_000792"
     CDS             3816..4094
                     /locus_tag="U4B29_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN2105759.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            4069..4539
                     /locus_tag="U4B29_000793"
     CDS             4069..4539
                     /locus_tag="U4B29_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN2105760.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            4556..4858
                     /locus_tag="U4B29_000794"
     CDS             4556..4858
                     /locus_tag="U4B29_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105761.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            4875..5246
                     /locus_tag="U4B29_000795"
     CDS             4875..5246
                     /locus_tag="U4B29_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN2105762.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            5218..5577
                     /locus_tag="U4B29_000796"
     CDS             5218..5577
                     /locus_tag="U4B29_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105763.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            5749..6756
                     /locus_tag="U4B29_000797"
     CDS             5749..6756
                     /locus_tag="U4B29_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2105764.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            6781..7965
                     /locus_tag="U4B29_000798"
     CDS             6781..7965
                     /locus_tag="U4B29_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN2105765.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(8402..9985)
                     /locus_tag="U4B29_000799"
     CDS             complement(8402..9985)
                     /locus_tag="U4B29_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2105766.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(10563..10706)
                     /locus_tag="U4B29_000800"
     CDS             complement(10563..10706)
                     /locus_tag="U4B29_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN2105767.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(10929..11408)
                     /locus_tag="U4B29_000801"
     CDS             complement(10929..11408)
                     /locus_tag="U4B29_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2105768.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(11481..11765)
                     /locus_tag="U4B29_000802"
     CDS             complement(11481..11765)
                     /locus_tag="U4B29_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105769.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(11843..12037)
                     /gene="ssrS"
                     /locus_tag="U4B29_000803"
     ncRNA           complement(11843..12037)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4B29_000803"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(12109..13395)
                     /locus_tag="U4B29_000804"
     CDS             complement(12109..13395)
                     /locus_tag="U4B29_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN2105770.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            13533..14204
                     /locus_tag="U4B29_000805"
     CDS             13533..14204
                     /locus_tag="U4B29_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN2105771.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            14170..14658
                     /locus_tag="U4B29_000806"
     CDS             14170..14658
                     /locus_tag="U4B29_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN2105772.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            14671..15618
                     /gene="prmA"
                     /locus_tag="U4B29_000807"
     CDS             14671..15618
                     /gene="prmA"
                     /locus_tag="U4B29_000807"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN2105773.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            15619..16383
                     /locus_tag="U4B29_000808"
     CDS             15619..16383
                     /locus_tag="U4B29_000808"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN2105774.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            16616..18829
                     /locus_tag="U4B29_000809"
     CDS             16616..18829
                     /locus_tag="U4B29_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN2105775.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            18840..19286
                     /locus_tag="U4B29_000810"
     CDS             18840..19286
                     /locus_tag="U4B29_000810"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN2105776.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            19457..20716
                     /locus_tag="U4B29_000811"
     CDS             19457..20716
                     /locus_tag="U4B29_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN2105777.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(20805..21752)
                     /locus_tag="U4B29_000812"
     CDS             complement(20805..21752)
                     /locus_tag="U4B29_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2105778.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            22146..23438
                     /locus_tag="U4B29_000813"
     CDS             22146..23438
                     /locus_tag="U4B29_000813"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN2105779.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            23453..25222
                     /gene="aspS"
                     /locus_tag="U4B29_000814"
     CDS             23453..25222
                     /gene="aspS"
                     /locus_tag="U4B29_000814"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN2105780.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            25331..26206
                     /locus_tag="U4B29_000815"
     CDS             25331..26206
                     /locus_tag="U4B29_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2105781.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            26472..28355
                     /locus_tag="U4B29_000816"
     CDS             26472..28355
                     /locus_tag="U4B29_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2105782.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(28430..28714)
                     /locus_tag="U4B29_000817"
     CDS             complement(28430..28714)
                     /locus_tag="U4B29_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2105783.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(28704..29438)
                     /locus_tag="U4B29_000818"
     CDS             complement(28704..29438)
                     /locus_tag="U4B29_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN2105784.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            29567..30190
                     /locus_tag="U4B29_000819"
     CDS             29567..30190
                     /locus_tag="U4B29_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN2105785.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            30382..31503
                     /locus_tag="U4B29_000820"
     CDS             30382..31503
                     /locus_tag="U4B29_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN2105786.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            31510..34638
                     /locus_tag="U4B29_000821"
     CDS             31510..34638
                     /locus_tag="U4B29_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN2105787.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            34657..37851
                     /locus_tag="U4B29_000822"
     CDS             34657..37851
                     /locus_tag="U4B29_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN2105788.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(37997..38704)
                     /locus_tag="U4B29_000823"
     CDS             complement(37997..38704)
                     /locus_tag="U4B29_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2105789.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        12598 a         6979 c         8223 g        11089 t
ORIGIN      
        1 aaatgtattt atttcatcgg tttttccaca aggtggaatc aattggaagc gagaaaatga
       61 aaataatcct ttgtcggtga tcttcaattc gggtatgaca gataatgtta agaaagaaag
      121 tgtcaaaaaa ggatcaaagg ttttagctgc tcctagttga tgtgctttag ttattaattc
      181 attcaactgt tcatagacga tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag
      241 agaaacacaa gctatttcct gctgtttgtt gacaacggtc atcccgccac ctttttgaat
      301 caaaagatta gcagcataca gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg
      361 cgagtcatgg gcaatcgttg tcgctatcgc tccttgtttc agctgaaatc ctttcactat
      421 gcctaatcca atgtttcccg tttgatggtg acgctcgatc accgcaatct ttaattggtc
      481 tttttcgatt gaaggaatga atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt
      541 agtcactaga ctattcggga taatttcgat cacatgggct tgtgaagaag aaagaagcag
      601 ctctaacgaa tgtggattta atggttgagc gtttagtttt ggcagacaac acgtatatga
      661 atttgaatga tcagcaaata aatggtcttg gagttgccca gattgaacaa ctaatgttcc
      721 cttatagaaa acttcatgaa ttggcagctt ggtaagatcg tctgttaaga agaaatcagc
      781 ttgataccca ggggcaatcg cacctagtgt tttcaaatca aaacattctg ctgcattcaa
      841 tgttgccatc tgaatggctt gtaatggatc gattccaagt gcaatagctt gacggacgat
      901 atgatcgatt gatccttctt ttactaaatc attgatcagt ttatcatcgg tgacaaagaa
      961 gcatcttctt gaattttcag gtgtgatcgc cggtagtagc gatgccagat cttttgcaac
     1021 ggtcccttct cgaaccatta aatacatacc aagatccaaa cgttctttag cttcttttgc
     1081 agttgtagct tcatgatccg tccgaatacc tgctgcagga taaacattta actcttccat
     1141 gccgatacca gctgcatgcc cgtcaatttt tttcttatgc tggtgcatca attggatctt
     1201 ttctaggatg tctgtttcat tatttgctac tgcttgatag ttcatgactt cagctaagcc
     1261 taaaacttcg ggtttttgta aaaaaggggc taattttttt gcatttaaat ttgcaccatt
     1321 ggtctcaaaa ggggttacag gtacacaaga aggtagcata aaaaagatat tcatgagtgt
     1381 ttgtcttgca tcttctatca taaactcaag acctgtactc ccagcaatgt ttcctatttc
     1441 atggggatct gtcactacag tagtaactcc atgaagcaag gatacttttg aaaattcgct
     1501 tggtgataaa agtgagcttt cgatatgcac atggccatcg atgaatcctg gcactagata
     1561 ttgcccatta gcattatgta cgctttttcc ttcgtattca ccgattccta cgatcattcc
     1621 ttcgcagatt gctacatctt tttccatgat ttctttcgta aagacgttga cgatcttagc
     1681 atgcttgatc actaggtcag caggcttttt tttacttgct acttcgatat atttccttaa
     1741 tgttgtttcc aatggagtgc ctcccaagat aaatttttat aagtataaca taaattcggt
     1801 ttttgccaat atttttttgt aaattcattt tatttgaaac aaaaagatca aaaagacgta
     1861 tgttttttgt ggaggggaga ataatggaaa taaaacgatt atcggatcaa atgataagtg
     1921 aaagttttgc caaacatgcc caagacgtat atatattgcc agcttcagaa gtattttaca
     1981 tctattttcg ctatggaaaa aaacgaacat tctggaagaa agtctcttcg gcagatggac
     2041 agcaactgat ctcaagattt aagtatttgg gacagatgga tatcggagaa aaaagaaaag
     2101 ctcagttagg tgcatttact tattctttag aaaaacagga agttcgtctg cggttgtctt
     2161 ctgtaggaga ttaccttcaa cgagagagtt tagtgattcg gctattatac ggtacttttg
     2221 aaacacccca tcgttgcttt agttctcgag attttgcaac tattttacag aaaaccgtca
     2281 gacgtggatt ataccttttc agcggaccag tcggatcagg caagacttct ctaatgtatc
     2341 ggttggcttt ggaagaaaaa ttgcaagtga ttactatcga agatcctgta gagatagaag
     2401 aaccgttttt tctgcagcta cagatcaatc cgaaaatagc acagacttat gatacacttt
     2461 tgagactcgc attacggcat cgtccagact tgctaatcat tggcgagata agagatgaat
     2521 caactgcaca tgctgcggta agagcagcac taacagggca ccgggttttt gcaaccgtac
     2581 atgcacgcaa tcttgaaggt acattggtca gaatccatga actgacaaag aaaaaagaag
     2641 agctatctga atgtttagct ggtgtcattt atcaagaact tttggaagat gccaatcacc
     2701 atccttctgt tttatggagt tatcaattta tggaggaaga atcagtaaaa aaatcatggg
     2761 aggaaagtta tgaagaagct caggaaaata tttggcagtc agaactattc taaaggatta
     2821 acaagaagcc agcaaaacca gtttgcacaa accatgggag aactgctttt atctggtttt
     2881 agtctgcaag aatcccttta tttattactc acgatccgtt cgtttccacc tgttattttg
     2941 gcaaatatcc aacatcagct aaaggaaggg caagcgttta gtgaggtact ttcgcaaata
     3001 ggatatggag cagagcaact ggtacagatc gagttagctg aacgtcatgg gaatctgccg
     3061 acaactttgt tggaaattgc caaacagttt cggcttgttg aaggatttcg caatgatttg
     3121 aaaaagctgc ttgcatatcc gatgctattg ctcacctttt tgctaggtat cttgtttagt
     3181 atgcggttgt tcgttttgcc tcagcttatt ggttcaggaa tggtcatgca agagcattgg
     3241 ggtattcagc tgatacaagt ctttcattgg tatgttctac tattcatcct agcggttact
     3301 ctattggctt tactagtaag gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa
     3361 tggataagca gacaattatt gattggaccg atatattcct tgtatcaatc ggcttatttg
     3421 tcactagaga ttggaaagct ttttcatgaa ggtttcgaat taaggcaaat catagtctgc
     3481 ttaaaggaaa cgaaacaaga aagtctgatt cagcatcttg cctctaagtt gattcaagga
     3541 ttggaggcag gtatgtcatt ggctgagcag ttccagaatt atcgttttct atcagaagaa
     3601 ttcagtaaaa ttgttttaca gggagaggca aagggaagtt taggtaaaga actgattttt
     3661 tacagcgaat ggacaagaaa acagcttttc aaacgtcttc atcactggat gcattttatt
     3721 cagccaatca tttttttggg cgtagcggct ttgattttat tagtttatgc cgctgtttta
     3781 ttacctgttt acggaagcat agaggaggtt ttgccatgaa aaagagaaaa atatcttatt
     3841 atagcggttt tacattgatc gaaatgctaa tcgtcttact gatcatcagt gtattagtgt
     3901 tgttgttcgt ccctaatttg tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag
     3961 cgatcatcca gttggtagac acacaaaaag agctctatgc gcttcaaaat aatggaagag
     4021 ttcctactgt ggaggaattg ttgaatgaag gctatatcaa acgagaacat gcagaaatct
     4081 atcagcggcc ttgaaggtaa ggttttagca tcaggatata cgttgtttga atcactctta
     4141 ctgattttat tacttacaac gatcctttct tttccagttc tcgcattttc tgcatggaag
     4201 aatgaattgg cagtctatca atttttcggc caatttgaaa aacgaatcta ttctacgcaa
     4261 aaaatcgcta ttgtgaatca gatccaaaca ggcttttatt ggaatagtga agagaatcaa
     4321 atcatttttc atgttccgaa tccagataaa ataaattggg aagtattaga tattcctgat
     4381 gagattacct tgaaacgcca tgcttctatt acttttgctg ctagaaccgg gaatgaaagt
     4441 tcactaaaag cttatcaatt ttattgggaa gaaaaagagc ggacgattac gtatcaattc
     4501 cagataggga gtggtcgcta tacaaaaaga attgaataaa acaggatttg ttttgttgga
     4561 gtcccttaca gcgcttatga tcgcttctgt ctgtgtctac ttagtgagct ttggccagct
     4621 gtttttgctt caggcagaaa agaaaagtgg ggaggaactg ctatttattc gaatgattta
     4681 tgaagatatc aagacgcagc ggatgtatca caaaagacca gagcgaaggg aagaaatgat
     4741 cccatacgat atccaaattg tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat
     4801 ggagaaagaa gcgatcagta ttgaaaaaac aagtaattcc agcatttacg ctgattgaat
     4861 gtctaattgg actgatgata ttgagcagtc tattgctcac attcaatctt ttgattcagc
     4921 aaaccatcca agtaagtgaa tttttgcagc gtaaagatca aaaagagtgg ctaatatttt
     4981 tagcacaatt agaagaagaa ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac
     5041 tgcactacac gatcgaggat aaacagtata tcatcgaaag atatcaggca atgatacgta
     5101 aaagaagaac ttccggcgga catcaaccta tgttaacaag tatcactgag cttcaattat
     5161 tggaaaaaga acacacaatt tctttttatg tacactttga aaatggagaa gatggatatg
     5221 ccatatggac aaaaaacgat cagtagaaaa tgttataagg gctctatttt attttctgtc
     5281 ttgcttttat ttcttttatg tacgttttta ttgatggcag cattagaaag ctaccgtttg
     5341 tcaatggatc ttaatatacg aatcaaaaat tattatatag caaaaataat gaagcagatg
     5401 gctttatcgg aacagtctac gcttgccgaa agttcggaag gagtttttta ctacacaact
     5461 ggatctgtaa catatcagtg ggtacaacag tccctattct tagaggtgga agtctcaccg
     5521 tttatctttc ggttcacaga agaagtaaaa aatgatacag atactagtac agaatgatgg
     5581 aatgttcgtg aaaacgccct cttttctgtt ataacactgt ttttaataaa aaaatgtttg
     5641 ccgtttcaca aataaaaaac agtgaaaacg attgaaattt tgtcaattgt tcgatagaat
     5701 taaacaggtg aaaaaattgt tataatatat ctagaaatga ggtgcaccat gttaccagaa
     5761 aaaatggaac aagggtttaa tcaaaacctg gaagctattc agcttttaca gaatgcattg
     5821 ggaacttcct ttttagaagc ctatgtcgaa aatgcagaaa atttaataga tgattatcaa
     5881 gtacgtgtgg tggatggagt gccgacaaaa gagacaacgc aacggatcac agctctttat
     5941 gaagagttga agaaactttc tttcgagcca gaagaatggc gcagactgtc tcaattactt
     6001 ttattgaagg gcagccagac agaacatttg caggcgaacc atcaactcac tcctgatagt
     6061 atcggttttt tatttgtttt tcttattgaa caactataca caaataaaaa agcaccggtt
     6121 aagattctgg atattgcggc aggaatgggc aacttgttat taacagttct tttaaaccta
     6181 tcgaatgcgg ggtaccaaac agaaggaatt ggtgtggata ttgatgatac attgttggct
     6241 gtcgcagctt ctacaagtga tttgacacaa gcaaatgttc aatactttca tcaagacggg
     6301 ttgcaggaat tattaataga tcctgtcgat tttgcaatca gcgatttgcc aatcggttat
     6361 tatccgaatg acgaaaaagc aaaagagttt ttaacaagta ccgaagaagg ccatagttat
     6421 gcacatcatt tattgctaga gcaatcaatg aaatatgtga agccggatgg ttttggtttg
     6481 tttttgatgc cgagtggatt tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt
     6541 aaagaagaag gttatcttca aggaatgatt caattgccag atgaattgtt ccgtaataaa
     6601 caatcgcaaa aaagtatttt gatcttacag aaaaaaggac cacaagcaca acaagtaaaa
     6661 gaagtcttgc ttgtgaaact tgcttcatta aaagaaccag agaaggtgac ggaatttttc
     6721 aacgagttta aaaactggaa atcatctgct ctgtaatcat tcaacgagaa ggagagtcat
     6781 atgtctaaaa caattgcaat caatgctggg agttcaagtt tgaaatggca attatatcaa
     6841 atgccagaag aaaccgtagt tgcgaaaggg atcgtcgaac gtatcggttt acaagattct
     6901 atttttacca tcaagtacgg cgataatcaa aaattcgaac aaatcatgga tatcgaaaac
     6961 cacgaagtag cagtaaaaat gctgttagat cagttgattg aactgcaaat tttaagctct
     7021 tatgatgaaa tcaccggagt agggcaccgt gttgttcacg gaggagaaac ttatggggat
     7081 tcagtagtga tcgatgagaa agtaatggac cgcattgctg aattagcaga atttgctcct
     7141 ttgcataatc ctgcaaactt aatggggatc aaagcgttta aaaagatttt acctgacatt
     7201 ttgagtgtgg ctgtattcga tacatctttc cactcaacaa tgccaaagca aaattattta
     7261 tatagcttac cggtgtctta ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc
     7321 acaagtcatc gttatgtgtc ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa
     7381 ctaaaaatca tcacttgcca cttaggaaat ggtgtatcga tcacagcagt cgatggaggt
     7441 aaatccatgg atacttctat gggattcaca ccattagctg gggtgacaat gggtacacgt
     7501 tcaggtgata tcgatccagc tgttctccca tatttaatgg aaaaattaga gattgatatt
     7561 gatgaaatga tcaatgtttt gaacaagaaa tctggtctgc taggcctaac aggattgtct
     7621 agtgatatgc gtgatcttga aaagaactat gaaaaagaac atattcgttt agcttatgac
     7681 attttcgtgg accgcatccg aaaatacatc ggcggttatg tgacaacgat gaatggtgtt
     7741 gatgcaattg tctttactgc tggtatcggc gagaatgacg gacatgtacg tagtgaaatc
     7801 atcaaaggta tgtcttggtt tggttgtgaa atcgatccag cgttgaatga attgcgtggt
     7861 gaagaagtag atatctctac aaaagaatca aaagtaagag tattagtgat cccaacagat
     7921 gaggaactaa tgattgcccg tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt
     7981 aggataaaag atgaaacgca tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa
     8041 gaccgcaaaa tggtaattta acttcaattt cccttctttt ttacaggatc ttcttaggga
     8101 attttgcttg gaaaagtggt caaaagggaa aagactggca ttttatttat tgattttcag
     8161 gaaaatccca atattttatt ttcattcatc tttataaggg acattacata tgtaaaaaga
     8221 aaaaacattt ttagatcagc ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc
     8281 tagaaaaccg atccgaaaca ttcattgatc gaataataaa aacttgattt tccacctgta
     8341 ctttgcctga atcgtgaaag cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt
     8401 cctagtcaat cagttggccg agcggctggc ggctgatctg ctcctgatca ataacgagtg
     8461 cttcttggaa gtgttgctgc aaaaagtagc ggtcatggct gactgctagg agcgttcctt
     8521 gatagtcttg cagcatttct tcaatttctt ctcgagcata gctgtcgagg tgattggttg
     8581 gttcatccaa aatcaggacg ttgatttttt ctaaaaagag tttcagcaac gccaaacgga
     8641 ttttttcacc accggataaa tctttgatcc gtttacccac atcgctttga taaaagccac
     8701 ttttggctaa ttgccgtcga gcgacttctt cgtttccagt caatgtgatg gcgtaagcca
     8761 gcaaacggct atcgggatcg gcaaactgca gctgctgcgg caaataaccg atttgcaagc
     8821 tactacctaa tttgatggtg ccaccgtaag gcttttcctc accgagtaat aatttgagta
     8881 aggttgattt tcctgcacca ttgtcgccga tgatggctaa atgatctcca cgatagactg
     8941 caaaactgct gtctttgaat agtacttgat cagcgtactg ctggtgcaag ttttgaatga
     9001 tgaaaacttc tttgccagat tttccaccat tagacaagct ttgcagccga tttttcgggg
     9061 gaggtggcgg tttgacgagt gtcagtttcg ccaggcggcg ttccagttct ttggcttttt
     9121 tgaagaagga ctcattgtct ccttcatgtg cccactgacg aaaacgacga atcatgactt
     9181 tcaaccgctg gacttctttt tgctgcagtt cgtaattttt tgtcagctca gccaagcgag
     9241 cttctttcaa gactcgaaaa cggctgtagt ttccgggata ctcaatcaat gcaccttctt
     9301 ccagttccca tgtttttgtg gtgacttgat cgagaaattg ccgatcatga gaaacgacca
     9361 gaaccgattt ttttgtattc tgtaaatagt tttccaacca gcgaatactg ttaagatcta
     9421 aatgattggt cggttcatcg agtaaaagta aatcggcatc ggctgctaaa attttggcta
     9481 aagcgacttt aactcgttcg cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg
     9541 gaatccctaa gccttgcaag atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt
     9601 cttcaaagcg atgctgcagc tcaccgtaac gggccagaca cttctctaag tttgtatctg
     9661 gttctgtcat caatgcttcg tagcgactca tttgctgttt gaggtcctgt atctcagcgg
     9721 cagaatggag caagtagtgg ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg
     9781 tcccgatctt taagcctttt ttgcgactga taacaccgct gtcgacacct tctctgccgg
     9841 tcaagatttg taaaaaggtt gatttgccag ttccgttgat accgactaag gcaatttttt
     9901 caccagcttc aatttgacag gataaatcct caaataatgg attcccgccg attgtttttt
     9961 taatagaaat tgcttgtatg atcatgatct ctctccttga gaccactcaa aaaggccatg
    10021 gttatccatg gcctgtgtta tttaaactca gattatcatg agaaaaatac cttgagatgg
    10081 ccattgattt tacgtgaatt caactgtttt ggacaaactt gtcccgtcaa cagcaactgt
    10141 aaaaccaatt gcatggctaa atgcgatatg gtatcgttgg taattttcat tacttgttat
    10201 tttagtcatg cgcatcatag tattttccct ccttcgtgta gttgtctttt ccagtgtacc
    10261 tgaaagtgtc agaaagttca actagcgctg acatttactt aatatttatc gatgtgtaat
    10321 tctgtcagag agcttttttt atctgactga gggtaatgtc tcgtggacca catcattgta
    10381 ttctgagcta aaccagcgtt caggcgaaat catatggtgt ttgataaaaa actttgtcta
    10441 catgacttcc ttttgccatt tgcttaatac cgttgcatta aacagctctt ctctttggaa
    10501 atctcaccaa catcgcgttc aggagtatcg ttgtggttgc aatggcaagc ggtagccttt
    10561 catcaattct tgaatgattt accgaatgta gcacttgcct tctcgcggta cccgaacccg
    10621 aaataaaatt ttagtttggc gataaaatcg gtatccgcca tcgatacttc atattttttc
    10681 gtatcagatt catcggaaac ttccacattg acgaaccaat aagctttggc atgcttaggt
    10741 cgttttaaac cacaccaggg cgactaatcg ccctggtgtt cacttttttt tattttactt
    10801 gaactcattg actgttttcc ctttatttta aacattataa agaccgatga gtgcttgccg
    10861 cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg
    10921 tctacgtgtt aagctttggt gtggtcttta gctggcaatt ggttttctaa atcagttcgg
    10981 acaacaagca catcgcaagc tgcgtttcta ataacatatt cagaaacaga accaataaat
    11041 aaacgttcta cagcattcaa tcctgtggct cctaacataa tcaaatctac ttgattatct
    11101 tctgggattt ctctggcaat aatttgtttt ggtgagccat attctacgac agaggttaca
    11161 ttattcaagc cagctttctt cgcattgctt tcataatctg ccaatgtttg ctttgccatt
    11221 tcagtagctt gttctgcaag cattccatca aaggatgaaa cggtttggaa agaacgtgtg
    11281 tcaatgacat gtgctaatag aagttctccg ttatttcgga tagctacatt gaccgccttt
    11341 ttaaaagcta attcagcttc atcagaacca tctacggcaa ccatgatttt tttgtattgt
    11401 tgtaacataa gcattcactc ctcaagtcaa tcgtattgta ctcttttatt gtagaacact
    11461 ttagggaaaa agagaattat ttactgcttt ggttcagcaa ttttttttgc aaatcggata
    11521 ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga ccaaggcaat aaagcgttga
    11581 ttgaaaaaag ctgttaaaat acccattacg cctaaaacag catatgcagc tccgtaaaat
    11641 tgtaggaatc cttgattttt atcatttttg ttgatcaata caaaaaagat atcctgttta
    11701 ttaaataaat aatagctaac gaataataat actaaagtgg cgataatcat taaaatccta
    11761 atcatgaaca aatcctccaa aataagcaac aaatagaaaa agcaatttgc caagagcaaa
    11821 ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt
    11881 gatcccgcga attcaagcag gtgggtccca tggtcgtagc ttcgaactac caaatgataa
    11941 ggcatgttac cagcagtgac acaagacggg tcccaagata tcaataaaaa tgttcggtca
    12001 acacacaaag aggacaattc gaacatcaca gatttcccac ctctatagta gcatatgaat
    12061 ttaaaaagag caatgagaga gctcgcagaa acagaaccgt tttcttgtct agttttttgg
    12121 aggatgcttt ttccactctt ttatacggta atactgttga cccagagcct gttcatattt
    12181 ccctgtatct tttggctgat aatactgagc attcttcagt ttatctggta aatattgctg
    12241 attgacccaa gcctgatcaa aatgatgagg atactgataa cccacgccac ggttgagtga
    12301 tttcgcacct ttgtaatggc tatctctcaa atgatcagga acatctccag ctttgccctc
    12361 tcgaatatca gcaagtgctg catctaatgc catgtaggca gaattggatt ttggcgacaa
    12421 gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc
    12481 agcagctaat acagcgttca ctgtacgagc cgcagcagca ggattaccta aacctatatc
    12541 ttcataaccg ataaccatca gacgccggca aatacttgcc agatcacctg cctcaactaa
    12601 acgggcgaga taatgaagtg cagcatccac atcacttcca cgaatcgatt tttgaaaagc
    12661 cgaaataacg tcataatgag catctccatt tttgtcatgg gtaagagctt ttctttggac
    12721 acattcttca atgatcgaca gtgtaagatg aatccttcct tctttatctg aaggagtcga
    12781 tagtgtagcc agttccagcc cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga
    12841 aaggtgaaga agagcatctt cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt
    12901 atcccttaaa gcatgttcga ctgccaactg gatgtcttgt tctgttaatg gtttgacttc
    12961 aaagatctgt gtacggctgc ggatcgctgg attgatggta atataaggat tctcagtagt
    13021 cgctccgatc aagatgattc ggccactttc taaatgagga agcagaaaat cctgttttgt
    13081 tttatctaat cggtgtactt catccaataa gaggatcact gtaccactca tttttgcttc
    13141 ttccgctaca acttgtagat ccttttttga atctgtagcg gcgtttagca tgcgaaatgc
    13201 ataattagta gagccggcga tcgcacttgc gatactcgtt tttccagtac caggaggtcc
    13261 atatagaatc atggaagaga gcatgcgagc atcgaccatg cggcgtatga tttttccttc
    13321 cccgactaga tgctgctgac caacgacttc atcgatcgtt cgaggtctca tgcgataagc
    13381 caatggctgt tgcataggat tttcctcctt cttgttttta cgtcccttaa ttatatcata
    13441 tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt gttttcatag cttatccaac
    13501 taatattgtg tatgataaag taggtgagaa aaatgacgga taaacaaaag atagatgaat
    13561 ttttttggaa taaaacgacg ccagaagtgg cacgggattt attggggatg tatttggagc
    13621 atgacacacc agaaggaaga ttagctggat atattgtaga cgcggaagca tacctaggtc
    13681 cagaagacga ggccgcccat agctatggtt taagaagaac accgcgtgtg cgggcaatgt
    13741 atgaaaaacc aggtacgatc tacctttaca cgatgcatac tcatcggatt ttgaacatca
    13801 ttactcagcc agaagggatt cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa
    13861 tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg tccagatatc agcaacggtc
    13921 cgggaaaatt agtggaagca ttagcaattc ctcaagaact ttatggtcag tctatagcag
    13981 atagttcgtt acgcttagtt tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc
    14041 ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt
    14101 cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat cgagaaagat tggggttgga
    14161 ggaaagaaaa tgaaaaagaa ggatctatta acatatttag atgaaataat tgaaaaacaa
    14221 gtgacagact atgatgtagc tttagattgg gatacaaaaa atcatacgat cgagatcgtt
    14281 gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg atgatgcaga agggatcgtt
    14341 tcgcaagaac caattatcga atttgaagac ggcgtacttc tttttaatcc tcagaaatct
    14401 gtttttgatg aacgcaatta tctagcagta atcccttatg aaggaaaaaa agggttggca
    14461 aaatcagtag ctgattcttt agtggaatat ctaaatgaag tattagctca agggcaaagc
    14521 gatttgcttg attttttgga tgaagacgat gacgaagctg tattcgagct tcactgggat
    14581 aatcagcaac tagcagaatt agtagaagta aaacagcaaa atgaagcaga tacttattta
    14641 ccgtacccaa gttattaagg agtgacagat atgaaatgga atgaagtaaa agtagaaact
    14701 gaaagtgaag cagtcgaagc agttgctaat attttgatgg aggcaggggc gagtggtgtc
    14761 gcgattgaag attctttaga tgttgaacat tttcagtcgg atcaatatgg tgagatttta
    14821 gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta ttttccggaa
    14881 acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca attacctgag
    14941 tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga aagcaactgg
    15001 gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg
    15061 ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac actagatcct
    15121 ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac agcactagaa
    15181 acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt
    15241 attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc
    15301 gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt ccatgtatca
    15361 gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc gaacatttta
    15421 gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca aacaggcacg
    15481 ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc catgttagaa
    15541 caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat tattttgaaa
    15601 aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga atcaaaagac
    15661 tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag gatggaaccc
    15721 gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga aatcacggag
    15781 attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca aaaggaactg
    15841 ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga ttggatcgtt
    15901 cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc agcctctgtc
    15961 gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa
    16021 gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt ggaaacgaaa
    16081 caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta tgaggaatca
    16141 gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa accagatagt
    16201 cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt cgattatttt
    16261 gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac agaaacagca
    16321 cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc ttctttcagc
    16381 taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata gtcaacaatt
    16441 tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa aaagctttta
    16501 aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac aattatcaaa
    16561 cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac
    16621 aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat acatgggtcc
    16681 cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac atgacggaca
    16741 gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag gtatattagc
    16801 agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg ttgttgaaga
    16861 tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg ctatgttagt
    16921 ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc agctagcaga
    16981 aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca tggtcaaatt
    17041 agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata agcaaagaag
    17101 gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag ggatcagccg
    17161 gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac agtattaccg
    17221 tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt
    17281 tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct atggacggcc
    17341 aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat
    17401 ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt atgctgtatt
    17461 aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt atattgccat
    17521 gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg caggaaatcc
    17581 ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg gggttgctgc
    17641 acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca
    17701 attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg cttctgaatt
    17761 tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca caccaaaagg
    17821 agacgttact gagttaccaa aaggatccgg accattggac tttgcataca gtatccatac
    17881 agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta
    17941 caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat ttggcccaag
    18001 tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca aacgtttctt
    18061 caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat
    18121 tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc aagaagcact
    18181 cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt atggagaagt
    18241 tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga
    18301 gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat
    18361 gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac taattcgtat
    18421 cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca ctaaaggcag
    18481 agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac caaatgtagc
    18541 agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag agtatgatgc
    18601 agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac ttcaaacagt
    18661 caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag ataaaatggc
    18721 gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta tcgtggataa
    18781 aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg aggacagata
    18841 tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg
    18901 gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat acgcaaaaag
    18961 atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa
    19021 aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct caattcacac
    19081 tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca cgaccagaaa
    19141 cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc atcacagtcg
    19201 ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga cctgtaacga
    19261 tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa aactatgaaa
    19321 cacgtcgctt aagttagact gataaactct aacttaagtg gcttttttgt ctgcaaaaag
    19381 cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa attatacaag ttatttttta
    19441 taggaggttg ctgaagatga aagaagtacc agtagtagat atcaaaatca cagatttaaa
    19501 aaaattgtat caaaaaggat acaacatgtt ggaggagtta cgccatgaag cggacgctcc
    19561 cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg atctacggat catcagcagc
    19621 aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt gctatgccaa aattagtatt
    19681 gaagacactt tttggtcaag gtggagtaca gactttggat ggagctgctc atcaccatcg
    19741 gaaaaatatt tttatggatc taatgacacc ggaaagaatg gaagattatc atcgtatctt
    19801 agataagaat ctgacacaag cacttgaagc acagcatggt caatttgaac tatttgattt
    19861 aagtaagatg gttttcttta catctatttg cgagtgggca ggtatcaatt tatcagcgat
    19921 ttctaaagat gaggtcgaaa aattagcaga atatcaaatc tctatgatca gcggaacgtt
    19981 tacttctccg attgatcaca taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc
    20041 gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt
    20101 atatgctttt gccaatgcca cagatttaga tggtcagctt ttacctctgg aagtggcagc
    20161 tgttgaatta ttgaacatca tccgtccaac ggttgctcta actgtgtggg cggcattgat
    20221 gggacatgca cttttcagta gaccagattt gtatcagcaa ttaaaaaatg acttttcgac
    20281 gttacaagat ccgtttattc aagaaatgag acgctattat cctttcttcc caatgctacc
    20341 tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt ataccagaag gcagttgggt
    20401 tattttagat ttatatggaa cagaccatga tgaacgaaca gttgaagcgc cagattcctt
    20461 tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc tataaagaag aatatgagat
    20521 gattgcacaa ggcgggggaa attttcgaca aatgcatcgc tgtgcaggag aatggatcac
    20581 tcttcatagt ctacgtgtgt tttccgatca gctagtaaat aagtttgagt tctctgtacc
    20641 tgaacaagat tggacgattc cattcaatca gtttccgact tatccgaata gccgagcgct
    20701 actttacaag aattagatta gcataaactt atcgaaaaaa aggaagatca aacagtgctg
    20761 atcttccttt tttcatatta ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat
    20821 gcttcatcaa tggtttgata atcttcattg gataaatgca aatctaaagc ttggacattg
    20881 tcagatactt gctctggacg cttagctcca ggtataacta cagacactcg tggattcttc
    20941 atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt atcttttagc aatggggcga
    21001 agtacatcga cagcggttaa aatttctctg aaacgttctc cttggaaatc tggatttctt
    21061 tttcgaggat ctccttcacc aaattgtttt tcctcaccaa gttcatattt tcctgtcagc
    21121 aaaccggaag caagaggaaa atagggaaca aaactgattt tgtttttttc aagataaggg
    21181 aataattctt tttcagcctg ccgatggatc aaactgtatt tatcttctac tacatcgaca
    21241 tatccatctg cattcgcttc tttcaattgt tctaacgtga agtttgacac accgactgct
    21301 cggatttttc ctgcttcttt taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg
    21361 ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat cagtttgcag acgttttagt
    21421 gcatcttcca ccgattgttt gagaaactca ggagaattat caaatgttcg ccctttattt
    21481 ggtacatgag cagcttttgt agcgatgacg acacgtgagc gatcatattc tttttccttc
    21541 agtacctcgc caatcagttc ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc
    21601 aaattgatcc cgctattcaa agctgtacga actacttgtt ttcctgtctc atcttccaaa
    21661 ccgtcaaaaa gattatgtcc tccaacggca tttgctccta atccaagctg ttctgcgtag
    21721 acttgtgaat gtccgattct tatttctgcc atagctgatt cctccttttt gtctagtcta
    21781 aaacaaaata ccaaaaagct gaactaaaac gtttttgtga ggggaaatag tcagatatct
    21841 cttgactttg agactggttt ttagtaggtt ataagtaata tcgaatgtca atgaaagagc
    21901 gagtaagttt ttgaaacttt acaagagagg atcatcgagg ctgaaaatga tctaaagttt
    21961 cattagccta aagacactct ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt
    22021 attcgtaaga ggaaagagaa atctttcaaa attaggtggt accgtgtatg aatttcattc
    22081 acccttgttc atgatgaaca agggtgattt ttttatttac atagctaaga aagggagaga
    22141 aaactatgag tttccaaaga ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa
    22201 aatggcagtt tgtcgaagaa acagcacgtt tattattcag agattatcag tataatgaaa
    22261 tgcgtacacc gatttttgaa cactatgaag tgatatcccg aagtgtaggg gatacaacgg
    22321 atatcgtttc aaaagagatg tatgattttt atgacaaagg ggaacgccat gtgacgcttc
    22381 gaccagaagg tacagcacca atcgttcgtt cttttgttga gcataaattg ttcggcccag
    22441 agtatgcgaa accgttcaaa actttttata tgggaccaat gtttcgctac gaacgccctc
    22501 aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga agcatttgga tcagaaaacc
    22561 ctgcaactga tgtggagagc atggtgatgg cacttgattt cttcaaacag ttggggatca
    22621 cgcaaatccg gctagtcatc aattcattag gtgataaaga aacgcgtcaa gcctatcgtc
    22681 aagcattgat cgactatctt ttaccttttg aatcagagct gagtgaagac tctaaacgcc
    22741 gccttcatga aaatccatta cgcgtattgg acagtaaaga caaacgagat caaaaatttg
    22801 tggcagaagc tccatctatc ttagattatc taagcgagcc tgctcaaaaa cattttgata
    22861 ccgttgtttc catgctggat gcattggctg ttccttatga aatcgacagt aatatggttc
    22921 gcggactaga ttattacaca catacgattt ttgaaatcat gagtgacgca ccaaaaatgg
    22981 gggcacaagc aacgatttgc gcaggcggac gttatgacaa tctagtggaa gaactaggtg
    23041 gtccttcaac acctggcttc ggatttgcaa tgggtatcga gcgattactc ttgactatgg
    23101 aggcagagga agtagttatc cctgcattta atgagttgga tgcttacgtg gtcgcactcg
    23161 gagacgaaac aaatatcgaa gcattgaaag tggtccaagc gattcgaaac tttggttttt
    23221 cagctgaccg cgacttcatg aaccgtaaag caaaagcgca atttaagaca gcagataaag
    23281 cgaatgccaa attagtattg gtgatcggtg aagatgagct tgctagtcaa acgattaatg
    23341 tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt agaagagata tatgaacatt
    23401 ttgatgaagt ctatgatgaa atgactttat tcgagtagga ggaaaaaaat aaatggcaca
    23461 aagaacgatc tattgcggat tagtttccga acaacaatta gaacaaacag ttacattaaa
    23521 aggatgggta caaaaaagac gagaccttgg gggagtaatc tttatcgacc tacgtgatcg
    23581 tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc
    23641 ggataaatgt cgcagtgaat acgtgatcga agtgactggg gaagttagaa gacgcggaga
    23701 aaatgcagta aatcctaaaa tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt
    23761 gattttgaat caagcaaaaa ccccgccatt tcaaattgaa gatgatcaag caatcagtga
    23821 cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat
    23881 caaattaaga aatgcgacaa caaaagcgat tcgtcattat ttagatgata atgactttct
    23941 cgatatcgaa acaccttacc tagggaaatc tacaccagaa ggcgcaagag actacctagt
    24001 tccttcacgt gtccatgctg gacatttcta tgcgttgccg caatctcctc aattgtttaa
    24061 acaattgctg atgaatgctg gattcgaccg ttattatcaa atcgttcgtt gtttccgcga
    24121 tgaagattta cgtggagacc gtcagcctga atttacgcaa gtagatattg aaacaacgtt
    24181 cttaacagca gaagaaatcc aaacttacac agagggattg attgcgaagg taatgaaaga
    24241 agttcgcggt atcgaagtca cattgccatt tcctcgtatg acctatgatg aagcaatggc
    24301 tcgctacggc agcgacaaac cggatacacg ttttgatatg gaattgatcg atctaagcga
    24361 tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt
    24421 gaaagcattg aatgctaaag gcgctgcaga tcgctactca cgaaaagaca tggatcagtt
    24481 aggtcaatat gtaggacaat tcggagctaa ggggcttgca tggctgaaag tagaagaaga
    24541 cggattgaaa ggtccaattg ctaaattcat gggagaagca acagaagcaa tcattaaagc
    24601 aacagatgca aaaccaggtg acttgctgat gtttggcgca gataaaagtg aaatcgtcgc
    24661 tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc
    24721 gaaattcaat ttcttatggg taactgattg gccacaattt gaattttcag aagaagaagg
    24781 tcgatatgtc tctgctcacc atccattcac gatgccaaaa gaagaagata tcccgttatt
    24841 ggctgaagac cctgcaaaag tctatgcaga agcttatgat atcgtcttga acggttatga
    24901 attaggcgga ggttctatcc gtattcatac tcgtgagctt caagaaaaag tactagaaac
    24961 tctaggcttt acaaaagaac aaatggaaga acaatttggt ttcttgttaa cagctttaga
    25021 ttacggattc cctccacatg gcggtattgc attaggattg gaccgttttg caatgttatt
    25081 agccggagaa gataatattc gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga
    25141 tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa ttgttcgaat tgaatattga
    25201 tgtaacgaat atagaagaat agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc
    25261 ttttgacaac cttgatttaa ttgaaaaaca ctttataatg aagccataac atataaagcg
    25321 gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat catgattaca cgaccaagct
    25381 ttcggtatct atcgtttatg cgttgatcgc atcagtcgca ttgaatttct tttatcagcc
    25441 aggacatatt tattcaagtg gtattactgg tctagctcag attttgacga ctttgtcaaa
    25501 atcactgata ggattcgaag taccggtatc aacaactttg tatttactga atataccttt
    25561 gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca attttcactt ttatcacagt
    25621 cactttgacc tccctttgta tccatatttt accggaaaca gtcctatcta ccgatccaat
    25681 catctgtgcg ttgtttgggg gggctgtgat gggactaggt attgggctag cgttgagaaa
    25741 cggcctatca tcgggcggtc tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa
    25801 gtcgattggt tctatttcga tctacttcaa tgcattgatc gtttttgttg ccggaattct
    25861 atttggctgg caatatatgt tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac
    25921 tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg atcgtaacga aacatccaga
    25981 aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg atcacgatca tcaatgaagc
    26041 agaaggtgct tataagcatg ataagcagac agtattgatg acgatcgtta cacgatacga
    26101 actgcctcta cttagagaag caatgaaagc ttctgatcca caagcttttg taagtattgc
    26161 agaaaacgtc cagatacttg gtcgtttcta tgaggatggc atttagatcg agtttgacaa
    26221 aaattcatga aatactgctg tagacagacg ttcgtttgtc tgcagttttt ttctaccata
    26281 gtagaatcag caaagaaaaa ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag
    26341 catgtttatt tttttaaatt tatgggatga tagaaaaaaa tagtagagaa aggacacgat
    26401 tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt tatgaaaagt
    26461 tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc actgggtttg
    26521 atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg ccaactagta
    26581 tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat ttgatcacgg
    26641 atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca
    26701 gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct tcggcttata
    26761 tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc agcagcttac
    26821 tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat cgatttgcta
    26881 atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac tatttggttt
    26941 ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg atcttttata
    27001 ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat acaccaggag
    27061 gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata tggttgggaa
    27121 gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga
    27181 aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt tgcttcttct
    27241 ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg agtctgttat
    27301 gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga tggctgacaa
    27361 atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat cagtttccag
    27421 ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg ttgcatactt
    27481 tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat gtggagcgtc
    27541 cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac
    27601 cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta gcaattgcac
    27661 tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg
    27721 caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca gaaaacacca
    27781 ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca caagggaata
    27841 aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat gctgcaacgg
    27901 atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga caattatatg
    27961 ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac actgttctga
    28021 ttggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg atggatgcaa
    28081 tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg
    28141 acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg ctgattgatt
    28201 ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc catccaaatc
    28261 ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga ggaagttcaa
    28321 gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact tcaggcaaaa
    28381 acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt catatagatt
    28441 caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt cgggtgagtt
    28501 ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact tctgcatgga
    28561 tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta ccattgttat
    28621 gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc tgatctttac
    28681 agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat ctctgcagtg
    28741 tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc gtctaatttg
    28801 ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt tgggtaaaca
    28861 aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat ttctaagaac
    28921 cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt acttgaaata
    28981 tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag gtgtttatta
    29041 gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac gactaagtca
    29101 caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg gacagtcatt
    29161 tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag acgaggttga
    29221 agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca
    29281 tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa gtgagcaagc
    29341 agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt gatatcttct
    29401 gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa
    29461 aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt gtcaaacctc
    29521 tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt tttatcgatt
    29581 tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc gctatgagaa
    29641 taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc gtacttggtg
    29701 ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta tggcaaaaga
    29761 agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg cttttccagt
    29821 taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt tgaaatcgac
    29881 aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac ttaaaggcgg
    29941 aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc
    30001 gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat
    30061 cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg aacgacgaac
    30121 acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat atgaagtcaa
    30181 aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg tcctgttcag
    30241 ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt tttgcgttcc
    30301 aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa taattagaag
    30361 gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca tatcggaaaa
    30421 aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc tatactagat
    30481 atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata tgataggagt
    30541 gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg gaatttaaag
    30601 gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag actttcagcg
    30661 ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc agaagcattt
    30721 gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga accaattgca
    30781 gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt agccaaggtg
    30841 atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat tagccatttt
    30901 tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg cgggttggat
    30961 tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca tttgcataat
    31021 caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa attctcttta
    31081 tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg cctggagttg
    31141 aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag tttcaaagaa
    31201 ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta tatctatctc
    31261 actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata tcctcgtatt
    31321 ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta
    31381 gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac ggaagagcag
    31441 ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat ggaaatggaa
    31501 tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt tatcaatgag
    31561 accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg gaagacagga
    31621 gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga aacttccgga
    31681 agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga agaaacaagc
    31741 gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa accagaacaa
    31801 gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt aacgttgacc
    31861 atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga agcagatcag
    31921 ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc
    31981 caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac tgttttacgt
    32041 catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa
    32101 caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca aaaaaggttt
    32161 gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc ccaatggaca
    32221 aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct aacggaactt
    32281 caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt
    32341 tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca aaaagacgaa
    32401 atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt agtcgaacca
    32461 atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag
    32521 caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa aaatgaagca
    32581 aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag
    32641 atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga acaattaaca
    32701 atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct cgctatctat
    32761 caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact gcaagaagat
    32821 cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa
    32881 aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa agcaactgaa
    32941 gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga acagctgaaa
    33001 agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc aggggagcca
    33061 tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga acagccaaat
    33121 aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga
    33181 actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca acaattatca
    33241 gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga agaacacttg
    33301 caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa aacaactctt
    33361 gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatgc aagaatagag
    33421 ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt ctacaaacag
    33481 cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa aagtttagaa
    33541 caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg acttgaaaaa
    33601 cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt gcaattgttg
    33661 caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat agaaaaaata
    33721 gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag ccagtcgtat
    33781 ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata taaagaaaac
    33841 caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg gctgcgtcaa
    33901 ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag ccaacagtta
    33961 gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt acaagaacaa
    34021 aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac aagccagacg
    34081 aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg agaaaatcct
    34141 aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt gttacaagta
    34201 gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct agcggatacc
    34261 attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga tcaggcaggt
    34321 atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc actttcttta
    34381 gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat cgatgcattg
    34441 tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc aatggaagct
    34501 ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt aagagaattg
    34561 aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca aagcaaaata
    34621 cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat tccagaaaaa
    34681 gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt tgtccctgac
    34741 caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat ggttgatttg
    34801 gatggtacag caaaagagat agattactgc caatgtccct attgggagga acataaatat
    34861 tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc tcgtatcatg
    34921 aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga aatcattgca
    34981 aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc tttgattatt
    35041 gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc tattttggca
    35101 ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt aaaaaacatc
    35161 tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa acagtaccag
    35221 tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca
    35281 ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa aggaaaacta
    35341 gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga gcagatgaat
    35401 cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat acaattttcc
    35461 gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta tttgctgcaa
    35521 gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat ccaagggaca
    35581 ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca attgattcgc
    35641 cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt gtttaaagag
    35701 attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt agaagaggaa
    35761 gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg aaaaatccaa
    35821 gctcgagtcg atttcgtcta tggtgaagtc atctattcga ctgacccgaa gcatgaggtc
    35881 tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg agtacttgat
    35941 ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc attgccaagc
    36001 ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca gtttggtgaa
    36061 gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca tcaaccgaaa
    36121 atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac agggatagag
    36181 gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt ttatacatta
    36241 gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag ccaagtgttg
    36301 caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc taaaaatcaa
    36361 ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga atcgttccaa
    36421 acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc caaaggattg
    36481 aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat
    36541 tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact acagacaatt
    36601 gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat cgtagcgcct
    36661 gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc tttaagtatc
    36721 caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga cacagatatc
    36781 cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc gcaaaaaatt
    36841 gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa
    36901 gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc aatcgagaat
    36961 aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt cccgactaaa
    37021 acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc gtttatttta
    37081 cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat gaatctatat
    37141 tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa
    37201 gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat acttgctggt
    37261 ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc
    37321 ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga gaacaaacga
    37381 agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga agagttagcc
    37441 gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga tcggttaagt
    37501 atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg
    37561 ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct ttggtggaat
    37621 cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa aaatgtggta
    37681 gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc actccagcaa
    37741 gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt gacgaaactg
    37801 actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta agagactcga
    37861 aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt atttgattta
    37921 aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat cattttttta
    37981 tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc tgatcaaaag
    38041 aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga tggttgaacc
    38101 aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa
    38161 cgtataaggt ctcttctgga agcatatgaa attctttctc agccagctga aaaatctctt
    38221 tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa ggaatccaat
    38281 tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca tttgtgataa
    38341 ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc tcctcatgca
    38401 agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg
    38461 taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct cgcatatatt
    38521 ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga tggcggaacc
    38581 ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt
    38641 ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca actgatttaa
    38701 tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt gactattgta
    38761 acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat atttctattt
    38821 ccttgacaga ataaggtttt tatatttcag tttttttcac taaaaatcgt gaaacacgtc
    38881 aataagttt
//
LOCUS       DASJTM010000017        37136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJTM010000017 DASJTM010000000
VERSION     DASJTM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.017"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..384)
                     /locus_tag="U4B29_000824"
     CDS             complement(<1..384)
                     /locus_tag="U4B29_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105790.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="U4B29_000825"
     CDS             complement(569..967)
                     /locus_tag="U4B29_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN2105791.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="U4B29_000826"
     CDS             complement(964..1737)
                     /locus_tag="U4B29_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2105792.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="U4B29_000827"
     CDS             complement(1734..2417)
                     /locus_tag="U4B29_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2105793.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="U4B29_000828"
     CDS             complement(2584..3471)
                     /locus_tag="U4B29_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2105794.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="U4B29_000829"
     CDS             complement(3468..4337)
                     /locus_tag="U4B29_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2105795.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="U4B29_000830"
     CDS             complement(4474..5760)
                     /locus_tag="U4B29_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2105796.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="U4B29_000831"
     CDS             6033..6611
                     /locus_tag="U4B29_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2105797.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="U4B29_000832"
     CDS             6611..7540
                     /locus_tag="U4B29_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2105798.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="U4B29_000833"
     CDS             7537..9273
                     /locus_tag="U4B29_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2105799.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="U4B29_000834"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="U4B29_000834"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN2105800.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="U4B29_000835"
     CDS             complement(10169..10678)
                     /locus_tag="U4B29_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN2105801.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="U4B29_000836"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="U4B29_000836"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2105802.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="U4B29_000837"
     CDS             complement(11979..13070)
                     /locus_tag="U4B29_000837"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN2105803.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="U4B29_000838"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="U4B29_000838"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN2105804.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="U4B29_000839"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="U4B29_000839"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN2105805.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="U4B29_000840"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="U4B29_000840"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN2105806.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="U4B29_000841"
     CDS             complement(17033..18007)
                     /locus_tag="U4B29_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN2105807.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="U4B29_000842"
     CDS             complement(17988..19871)
                     /locus_tag="U4B29_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN2105808.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="U4B29_000843"
     CDS             complement(19889..22114)
                     /locus_tag="U4B29_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2105809.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="U4B29_000844"
     CDS             22229..23263
                     /locus_tag="U4B29_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2105810.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="U4B29_000845"
     CDS             complement(23330..24343)
                     /locus_tag="U4B29_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2105811.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="U4B29_000846"
     CDS             complement(24421..24609)
                     /locus_tag="U4B29_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN2105812.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="U4B29_000847"
     CDS             complement(25049..25177)
                     /locus_tag="U4B29_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2105813.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="U4B29_000848"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="U4B29_000848"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2105814.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="U4B29_000849"
     CDS             complement(26664..27719)
                     /locus_tag="U4B29_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2105815.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="U4B29_000850"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="U4B29_000850"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN2105816.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="U4B29_000851"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="U4B29_000851"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN2105817.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="U4B29_000852"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="U4B29_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN2105818.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="U4B29_000853"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="U4B29_000853"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN2105819.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="U4B29_000854"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="U4B29_000854"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN2105820.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="U4B29_000855"
     CDS             35481..37040
                     /locus_tag="U4B29_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN2105821.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10262 a         8064 c         6697 g        12113 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatctac
    37081 actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc cagcct
//
LOCUS       DASJTM010000018        36998 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJTM010000018 DASJTM010000000
VERSION     DASJTM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.018"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..286
                     /locus_tag="U4B29_000856"
     CDS             <1..286
                     /locus_tag="U4B29_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105822.1"
                     /translation="KSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFI
                     RMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            345..785
                     /locus_tag="U4B29_000857"
     CDS             345..785
                     /locus_tag="U4B29_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105823.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            797..1465
                     /locus_tag="U4B29_000858"
     CDS             797..1465
                     /locus_tag="U4B29_000858"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2105824.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1529..2152
                     /locus_tag="U4B29_000859"
     CDS             1529..2152
                     /locus_tag="U4B29_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN2105825.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2208..2594
                     /locus_tag="U4B29_000860"
     CDS             2208..2594
                     /locus_tag="U4B29_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN2105826.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2807..4198
                     /locus_tag="U4B29_000861"
     CDS             2807..4198
                     /locus_tag="U4B29_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN2105827.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVATQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4809..5177
                     /locus_tag="U4B29_000862"
     CDS             4809..5177
                     /locus_tag="U4B29_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN2105828.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5357..6298
                     /locus_tag="U4B29_000863"
     CDS             5357..6298
                     /locus_tag="U4B29_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN2105829.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6367..7098
                     /locus_tag="U4B29_000864"
     CDS             6367..7098
                     /locus_tag="U4B29_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN2105830.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7242..9422
                     /locus_tag="U4B29_000865"
     CDS             7242..9422
                     /locus_tag="U4B29_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN2105831.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9442..9924
                     /locus_tag="U4B29_000866"
     CDS             9442..9924
                     /locus_tag="U4B29_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN2105832.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9940..10701
                     /locus_tag="U4B29_000867"
     CDS             9940..10701
                     /locus_tag="U4B29_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2105833.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10832..12166
                     /locus_tag="U4B29_000868"
     CDS             10832..12166
                     /locus_tag="U4B29_000868"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN2105834.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12473..12958
                     /locus_tag="U4B29_000869"
     CDS             12473..12958
                     /locus_tag="U4B29_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN2105835.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12951..13634
                     /locus_tag="U4B29_000870"
     CDS             12951..13634
                     /locus_tag="U4B29_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN2105836.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13760..16405
                     /gene="polA"
                     /locus_tag="U4B29_000871"
     CDS             13760..16405
                     /gene="polA"
                     /locus_tag="U4B29_000871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN2105837.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16450..17286
                     /gene="mutM"
                     /locus_tag="U4B29_000872"
     CDS             16450..17286
                     /gene="mutM"
                     /locus_tag="U4B29_000872"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN2105838.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17250..17879
                     /locus_tag="U4B29_000873"
     CDS             17250..17879
                     /locus_tag="U4B29_000873"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN2105839.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17931..18263
                     /locus_tag="U4B29_000874"
     CDS             17931..18263
                     /locus_tag="U4B29_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2105840.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18575..19066
                     /gene="nrdR"
                     /locus_tag="U4B29_000875"
     CDS             18575..19066
                     /gene="nrdR"
                     /locus_tag="U4B29_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN2105841.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19089..20462
                     /locus_tag="U4B29_000876"
     CDS             19089..20462
                     /locus_tag="U4B29_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2105842.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20462..21388
                     /gene="dnaI"
                     /locus_tag="U4B29_000877"
     CDS             20462..21388
                     /gene="dnaI"
                     /locus_tag="U4B29_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN2105843.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21517..21957
                     /locus_tag="U4B29_000878"
     CDS             21517..21957
                     /locus_tag="U4B29_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2105844.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22101..22802
                     /locus_tag="U4B29_000879"
     CDS             22101..22802
                     /locus_tag="U4B29_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN2105845.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22799..23920
                     /locus_tag="U4B29_000880"
     CDS             22799..23920
                     /locus_tag="U4B29_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN2105846.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23935..25167
                     /gene="pepT"
                     /locus_tag="U4B29_000881"
     CDS             23935..25167
                     /gene="pepT"
                     /locus_tag="U4B29_000881"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN2105847.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25299..26207
                     /locus_tag="U4B29_000882"
     CDS             25299..26207
                     /locus_tag="U4B29_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN2105848.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26242..26514
                     /locus_tag="U4B29_000883"
     CDS             26242..26514
                     /locus_tag="U4B29_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN2105849.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26525..27571
                     /locus_tag="U4B29_000884"
     CDS             26525..27571
                     /locus_tag="U4B29_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN2105850.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27822..30431
                     /gene="clpB"
                     /locus_tag="U4B29_000885"
     CDS             27822..30431
                     /gene="clpB"
                     /locus_tag="U4B29_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN2105851.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30582..31268)
                     /locus_tag="U4B29_000886"
     CDS             complement(30582..31268)
                     /locus_tag="U4B29_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105852.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31246..31740)
                     /locus_tag="U4B29_000887"
     CDS             complement(31246..31740)
                     /locus_tag="U4B29_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2105853.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31760..32980)
                     /locus_tag="U4B29_000888"
     CDS             complement(31760..32980)
                     /locus_tag="U4B29_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105854.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33127..34962
                     /gene="lepA"
                     /locus_tag="U4B29_000889"
     CDS             33127..34962
                     /gene="lepA"
                     /locus_tag="U4B29_000889"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN2105855.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35158..36069
                     /locus_tag="U4B29_000890"
     CDS             35158..36069
                     /locus_tag="U4B29_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105856.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        12482 a         6400 c         7919 g        10197 t
ORIGIN      
        1 aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt
       61 gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat
      121 ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga
      181 cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag
      241 aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt
      301 ggattttttt gtcataataa atattagatg atggaggaga taatatgatg caaacatggt
      361 tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga
      421 aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc
      481 gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat
      541 ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat
      601 atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg
      661 atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta
      721 aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg
      781 tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt
      841 tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc
      901 agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc
      961 tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg
     1021 gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa
     1081 aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg
     1141 atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt
     1201 ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc
     1261 cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg
     1321 tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat
     1381 ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac
     1441 aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa
     1501 agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac
     1561 attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg
     1621 tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt
     1681 gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa
     1741 agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa
     1801 agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct
     1861 gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt
     1921 tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag
     1981 aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg
     2041 agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt
     2101 ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct
     2161 ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa
     2221 gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag
     2281 aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag
     2341 aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat
     2401 ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg
     2461 tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt
     2521 cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag
     2581 aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt
     2641 ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat
     2701 aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt
     2761 tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat
     2821 cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa
     2881 gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg
     2941 cggtctgctt caattgatct ttggtgtaga cgacaaagtc gctactcaat caatggaatg
     3001 gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt
     3061 gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat
     3121 tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat
     3181 tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac
     3241 agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca
     3301 gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac
     3361 aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa
     3421 aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat
     3481 cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat
     3541 gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt
     3601 tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc
     3661 aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac
     3721 ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg
     3781 agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg
     3841 ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa
     3901 tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg
     3961 tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa
     4021 cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg
     4081 tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa
     4141 agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc
     4201 aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat
     4261 agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg
     4321 tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     4381 tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac
     4441 cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa
     4501 aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa
     4561 caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca
     4621 tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt
     4681 atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat
     4741 tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga
     4801 ggaaacagat gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa
     4861 atgcaacgtt ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga
     4921 cgcttataat tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag
     4981 catttgttga gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca
     5041 gtaaagatat ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata
     5101 tggtacagct tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg
     5161 cggttacagg taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga
     5221 agtcttttta attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata
     5281 cacaagcaaa taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga
     5341 aggaaggaag aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga
     5401 agaaccagag gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg
     5461 tcgtattcta gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac
     5521 gactgcctat atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat
     5581 cgatccaagc gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt
     5641 aagtttaaag ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc
     5701 gcaagaattt aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga
     5761 ttatgaatat gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg
     5821 agagcttcca gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac
     5881 ctactgtact ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt
     5941 caaagatgtc ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca
     6001 aggagaatta tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa
     6061 ccaagtagat aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata
     6121 tatcaattgt agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga
     6181 aaaacattta ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca
     6241 agaccatgga atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa
     6301 aacaatagtt ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg
     6361 atccacatga aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt
     6421 gaaaaactta ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc
     6481 aatgatcgag gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa
     6541 acaggagcca tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca
     6601 ccggaggact ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg
     6661 attcacccag gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg
     6721 agatattcag aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat
     6781 tatctttttg tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta
     6841 agagacttta tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca
     6901 attgtatttg gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc
     6961 tggattcagg atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat
     7021 ggggttgttt ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga
     7081 ccttggcatg gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct
     7141 ttttattttt atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga
     7201 tagaataaag aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc
     7261 agacgatcat tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta
     7321 ctgtacttaa tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag
     7381 aaatgactgg aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc
     7441 ttgaaaactt agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac
     7501 tgactcccga gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt
     7561 tgtatcgtcc atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac
     7621 ttgaaccact cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag
     7681 aaaaatatat tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc
     7741 atgagattat tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt
     7801 atacgtataa caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag
     7861 gcgtatatga aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa
     7921 tattagctac taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg
     7981 aagcagcaat tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt
     8041 cttcattcgt cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg
     8101 agcgagaact gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg
     8161 gcgaaaacct ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat
     8221 ttgatccggc ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt
     8281 tagcaatcga agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg
     8341 gaccagcttt tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg
     8401 gtacagccag tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata
     8461 aaacctatta cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg
     8521 cacgaaaaga atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc
     8581 gcctgcaaga tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc
     8641 agtaccaaca tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa
     8701 cagcggttaa ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata
     8761 tatcaggatt gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag
     8821 aattcaccgc acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac
     8881 aagcaatcgg attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc
     8941 atcccgaaag ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag
     9001 agcttggtac agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg
     9061 aaagcctatc agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg
     9121 gacgagatat gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg
     9181 aagacttgaa accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg
     9241 catttgttga tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa
     9301 aattcgtcaa gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag
     9361 aacaagtcga tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt
     9421 aacaatgagg agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata
     9481 tcattaaaaa gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt
     9541 acaactggcg ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt
     9601 gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat
     9661 cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa
     9721 aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat
     9781 tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa
     9841 tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt
     9901 aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg
     9961 aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt
    10021 gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat
    10081 aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg
    10141 gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat
    10201 ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat
    10261 aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg
    10321 gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca
    10381 ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc
    10441 gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca
    10501 gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa
    10561 atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct
    10621 cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa
    10681 acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg
    10741 gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag
    10801 aatggacgct agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt
    10861 tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct
    10921 aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc
    10981 aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc
    11041 aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga
    11101 agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt
    11161 aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt
    11221 ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt
    11281 cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc
    11341 aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt
    11401 tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga
    11461 tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa
    11521 cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta
    11581 tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt
    11641 aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc
    11701 agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga
    11761 catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg
    11821 cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac
    11881 gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta
    11941 cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct
    12001 tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt
    12061 agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca
    12121 agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag
    12181 tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg
    12241 caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca
    12301 aaaacatgag aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt
    12361 cacgacctct gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg
    12421 ctacttttgg tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat
    12481 aggtaagccg agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc
    12541 cttaacagaa tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc
    12601 aaatcctttg tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt
    12661 tccatcagtg atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc
    12721 aggatcacat gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac
    12781 actgactttt cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc
    12841 tgtagaagcg gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc
    12901 gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc
    12961 caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta
    13021 tgggacttgc ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat
    13081 tagcaacttt tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga
    13141 ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat
    13201 ttattgccta ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata
    13261 ttggaacagt aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg
    13321 tcggagtaac aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa
    13381 tcggagtgat cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc
    13441 tgttcatctc cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa
    13501 gcatccgtaa tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca
    13561 tggcattgaa gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg
    13621 gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta
    13681 tggtcccgct ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt
    13741 acagtaagga gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt
    13801 ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg
    13861 tcttcataca aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga
    13921 gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat
    13981 gtatgcggag tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc
    14041 atatatacgt gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga
    14101 agcagatgat attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt
    14161 cgtgctttca ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat
    14221 cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata
    14281 tgatgggttg acgccaaaac agattattga catgaaggga ctagccggag atgcctctga
    14341 caatatcccc ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata
    14401 tggctcagtc gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga
    14461 aaatctgata aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac
    14521 ttcagcgcct gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa
    14581 actcgttccg ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt
    14641 agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga
    14701 aatgtttact acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga
    14761 ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc
    14821 tgtattcgaa aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt
    14881 atatgatgcg aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat
    14941 cgattttgat gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat
    15001 cgaaggaata gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg
    15061 caaaggggcg aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg
    15121 aaaagtagct gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa
    15181 ccaagaagat cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga
    15241 aataagcggg atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc
    15301 tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa
    15361 ttcaccaaag caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa
    15421 aacaaaaact ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc
    15481 aatcgtagag gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga
    15541 gggactgctg aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt
    15601 gacccaaaca ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt
    15661 agatgaaggg agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta
    15721 ttcttctgac tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca
    15781 tttgaaagct gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt
    15841 tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa
    15901 ttttggtatc gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg
    15961 aaaagcagca cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata
    16021 tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg
    16081 ccgtcgatat ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg
    16141 aacagctatc aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat
    16201 cgaattggat aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca
    16261 tgatgaactt gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa
    16321 ggaagttatg gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg
    16381 aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga
    16441 aaagaaaaaa tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta
    16501 gtagtaggaa aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca
    16561 gagacaccca tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga
    16621 gggaagtttt taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa
    16681 ggaaaatatc aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc
    16741 ttttttgagg atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca
    16801 atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct
    16861 ttgccagata gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca
    16921 atcaagccat tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat
    16981 gaagcattat gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa
    17041 gaggtagaac aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga
    17101 ggtaccacga ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca
    17161 ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa
    17221 acaaaagtgg cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca
    17281 aagtaggatt tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca
    17341 aagtctttca gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg
    17401 ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc
    17461 agcctgatgg gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa
    17521 aacggcaaaa acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa
    17581 tcgcctctct aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag
    17641 gtcattatga ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc
    17701 ttcagcgttt gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta
    17761 gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa
    17821 caaaagaaaa acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag
    17881 tatctgtgtc aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat
    17941 taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca
    18001 ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa
    18061 agcagtacca agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc
    18121 ggcaagattt gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc
    18181 cagaaagtga ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata
    18241 caggaatcga agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga
    18301 gaaggtgttc atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag
    18361 ttatttctca gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg
    18421 acacaattac gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac
    18481 agtcgcgttt gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc
    18541 tttatataaa atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa
    18601 taattcacga gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg
    18661 cgaatgtgag aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt
    18721 attagtaatc aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg
    18781 actcatccgt tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa
    18841 ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg
    18901 tgaatatgtg atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag
    18961 tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga
    19021 taaagcaaaa gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag
    19081 gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa
    19141 aagcggctcc gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa
    19201 tcggcggaga agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg
    19261 gcaaaggtcc tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc
    19321 aattttatga tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag
    19381 aagatagtga tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag
    19441 catttttcaa agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat
    19501 tccagaagct tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa
    19561 tcacaaaaaa atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag
    19621 aaaacatcga atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag
    19681 cagattcgac attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa
    19741 gtcttgatga tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg
    19801 aattgacact tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg
    19861 aaaaagaact gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag
    19921 aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg
    19981 cagcgcttca gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa
    20041 tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg
    20101 tttctcaatc aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga
    20161 tcaagaataa tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac
    20221 aaaagaaaat cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag
    20281 caaaacaatc aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac
    20341 gggaaaagct tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga
    20401 aaaaagcaga gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct
    20461 agtggaagac gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa
    20521 gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc
    20581 acaccaagaa gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt
    20641 cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga
    20701 gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt
    20761 gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa
    20821 agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag
    20881 cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg
    20941 actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag
    21001 agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga
    21061 gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc
    21121 acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga
    21181 agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc
    21241 gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga
    21301 gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat
    21361 gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta
    21421 gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc
    21481 aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata
    21541 tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt
    21601 tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga
    21661 caaagcatat ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg
    21721 cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca
    21781 ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat
    21841 gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg
    21901 tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca
    21961 ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta
    22021 aggagatatg ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat
    22081 tatctagaag gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg
    22141 gtgactttgt ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac
    22201 ccgttgcatt gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag
    22261 gtccttatga atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg
    22321 tacgccttgc aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga
    22381 ttgcgggtat gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac
    22441 gcttaagcgg acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat
    22501 cttggctgca agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa
    22561 aaatatatga aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg
    22621 agctgatgtt cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga
    22681 caagagagct tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag
    22741 aggcacgtca aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat
    22801 gacaatcagc ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc
    22861 tgaagaagga gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt
    22921 aatgatgaca ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga
    22981 tttgctgatt gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca
    23041 gccacaagaa aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca
    23101 cacgaacatg gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat
    23161 tgaagtggag agttatttag taaagactca tgagattcat tataaaaaac tggccgttta
    23221 tgtacctgtg gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac
    23281 acaaggtgat tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga
    23341 agctggtgca caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa
    23401 agttgaggtt attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc
    23461 tcatccttat gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt
    23521 tgggttagga cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca
    23581 agtaaaagaa gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc
    23641 ctctgtgaag cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat
    23701 cgcccaaaga gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat
    23761 gcaaagcgct gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca
    23821 acgatttatc gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt
    23881 cgtttctgaa acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac
    23941 gaaaatcttt tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca
    24001 agcaaaacga ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa
    24061 ttagaagagt taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga
    24121 acattaccaa gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat
    24181 acagcagatt ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag
    24241 tctacgatcg tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat
    24301 ttgaaaaatt atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca
    24361 gatgacaaat cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct
    24421 tctatccctc acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga
    24481 gcggataaat tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt
    24541 cctttaggtg agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa
    24601 gggaaaaatg tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca
    24661 atcgattttc ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa
    24721 ggtttcttcc atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc
    24781 attcgcgatc atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa
    24841 gaagagttga atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat
    24901 tacaacatga aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca
    24961 atgatcgagc ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca
    25021 aaaatctctt acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac
    25081 ggacgattcg aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa
    25141 attgcagaat tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc
    25201 gtcccaatga tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt
    25261 cccgccctct tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt
    25321 ataacaaaag ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg
    25381 taaaagaaaa gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg
    25441 atgcggaatt agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg
    25501 gagacggcac gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg
    25561 tcggattact tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca
    25621 aaaaagaaga agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc
    25681 aagtcaatga tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag
    25741 tttggatcag tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt
    25801 ttaagttatt gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt
    25861 ggaaaggaaa atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa
    25921 attttgacga ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct
    25981 tagatatttt ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga
    26041 tccccggcat tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg
    26101 tgggcactcg aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat
    26161 caaaaggatt aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa
    26221 cagaaagttg gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt
    26281 ttcattgtga atttttactt attttgtctg atggggtatg ataaacatca ggctagaact
    26341 agcggctggc gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg
    26401 ggaggattga tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata
    26461 ggattcgctg caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca
    26521 ggagatgtcg tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt
    26581 gatttttgcc attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt
    26641 gctgtctacc agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat
    26701 cgctgaaggg cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa
    26761 agatgggttc ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg
    26821 aacgctgatc tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc
    26881 aggggtgaca gcactccata tgattatgta caaactggca gtgatcactt atgcattagc
    26941 tggcttggtc atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat
    27001 catcgctggt atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag
    27061 cttgaatgtt caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt
    27121 gcggaaatgg gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac
    27181 cattatccaa gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt
    27241 attttggtat gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct
    27301 actgaccatt tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc
    27361 tggtattggt tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt
    27421 tgacgcagtt tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta
    27481 tggatttgtg tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt
    27541 aaaaaaagag aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca
    27601 aatcaaagaa aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat
    27661 acatacaatc aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac
    27721 tatgtgtata ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa
    27781 gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga
    27841 ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc
    27901 aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa
    27961 atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac
    28021 tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga
    28081 atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc
    28141 tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt
    28201 atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag
    28261 gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg
    28321 gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg
    28381 aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga
    28441 taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac
    28501 gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat
    28561 tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag
    28621 aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag
    28681 gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc
    28741 gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa
    28801 aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag
    28861 atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca
    28921 tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt
    28981 tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa
    29041 tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg
    29101 aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc
    29161 aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag
    29221 aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg
    29281 aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta
    29341 cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga
    29401 ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa
    29461 cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg
    29521 aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg
    29581 taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc
    29641 taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg
    29701 actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt
    29761 ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg
    29821 aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc
    29881 ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag
    29941 ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt
    30001 tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca
    30061 acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt
    30121 tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat
    30181 tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt
    30241 ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca
    30301 ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat
    30361 caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag
    30421 aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt
    30481 cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg
    30541 aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt
    30601 tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat
    30661 cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct
    30721 gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag
    30781 aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca
    30841 aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag
    30901 taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt
    30961 ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg
    31021 aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc
    31081 aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg
    31141 attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg
    31201 aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt
    31261 ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt
    31321 tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc
    31381 tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt
    31441 atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc
    31501 ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc
    31561 tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc
    31621 attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa
    31681 ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat
    31741 ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga
    31801 tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat
    31861 aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg
    31921 attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga
    31981 agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg
    32041 ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat
    32101 atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt
    32161 tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata
    32221 agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag
    32281 ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg
    32341 tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata
    32401 aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa
    32461 ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga
    32521 ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag taggggatac
    32581 tgtaaggata caaataagag atccattgct tattcttgcg catattatcc aatgcaacga
    32641 taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca
    32701 caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt
    32761 gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt
    32821 ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag
    32881 tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg
    32941 gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta
    33001 gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa
    33061 ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga
    33121 gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc
    33181 atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac
    33241 actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa
    33301 cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa
    33361 acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct
    33421 cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa
    33481 gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc
    33541 atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac
    33601 gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt
    33661 gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca
    33721 ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat
    33781 gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc
    33841 aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt
    33901 ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga
    33961 gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga
    34021 aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat
    34081 ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa
    34141 acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat
    34201 gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc
    34261 gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat
    34321 tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg
    34381 gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc
    34441 attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct
    34501 gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg
    34561 gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat
    34621 ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga
    34681 aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc
    34741 caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac
    34801 gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa
    34861 aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc
    34921 atggccgttc tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa
    34981 acctattaaa taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt
    35041 ttaaactgtc atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct
    35101 tgaagatttt tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg
    35161 gttgataatt caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa
    35221 aaaaagttag ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg
    35281 tcaagaaaaa tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact
    35341 ctaataaaag atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga
    35401 gatatgaata agttacttaa caaagagtta gctgataata taggaattag tgaagtatta
    35461 ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat
    35521 atttttctat taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata
    35581 tttagtttat caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag
    35641 aaccgggaag agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat
    35701 gttataagta gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt
    35761 gcctatcttg gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata
    35821 cttccaaaag atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta
    35881 aggatgagga aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt
    35941 tctccttcaa tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat
    36001 actaatcgaa ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac
    36061 gggatataaa aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa
    36121 aaaatttata aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt
    36181 gctttactgg cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg
    36241 aagaagtaat acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg
    36301 ttatccaaca atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga
    36361 tgagtattga tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg
    36421 tcctctgcag cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat
    36481 acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg
    36541 actgaattct tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt
    36601 ggggatcttt tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt
    36661 tctctaaaaa aaacggcatc gaatgggttg ggtgattgat aagcaatgaa tcatagactg
    36721 atatagaata aaaaatgatt ctatttatct ttttacatct atcgtttttt caagtagccg
    36781 caaattatat cagcattcaa caatttttgt atacaagttc tagtaaaagg atctgaagaa
    36841 aaagttggtt gttgtgaaga taaaaaaaga atcaggtttt actgagtttt tcttcaaaat
    36901 ttgtgttatt aggttaggac aaagataaga agaagtatga agatttaaaa aaggaatgag
    36961 gaaaagatag taatttccga ttattggagt ttttttat
//
LOCUS       DASJTM010000019        36574 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJTM010000019 DASJTM010000000
VERSION     DASJTM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.019"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..3231
                     /locus_tag="U4B29_000891"
     CDS             <1..3231
                     /locus_tag="U4B29_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN2105857.1"
                     /translation="MLSTPVLISVVEVAANEQQSVNEENAHVNIEGMSTMEEIPRTSE
                     DMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSASRYAIEPLEDGVRLSS
                     TNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYTNAQEGFALMVRDAVPK
                     NHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMRLGLGTRAITGISSEDP
                     QAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFPATNSITKLDVEMKKTN
                     TGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAARNMTIKVEGIEVAYSD
                     PATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADGLLTIKNKATGEVLKNA
                     KNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEMTSYETKEILHTVDYRK
                     PKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMAPGTYSFERGLTIPKGV
                     NGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLRGFDITNSAAMQKGLQI
                     SGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNCTSFENADPGFEDADGF
                     AAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIKNSVAYRNGWVPGIEGE
                     GNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIVNSSTSFNNGSYNVALY
                     TSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSLNYYWNEREKQSENTLG
                     HTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGNRQTEKERVDGLPLSVG
                     AIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGVAENAEGNIIELKPFTV
                     FVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIFSAPSKGEYTLEVTYRS
                     EIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPGNESPNGKPNGKPSHPD
                     RTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMKQRKK"
     gene            complement(3772..5679)
                     /locus_tag="U4B29_000892"
     CDS             complement(3772..5679)
                     /locus_tag="U4B29_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN2105858.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5809..6684
                     /locus_tag="U4B29_000893"
     CDS             5809..6684
                     /locus_tag="U4B29_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN2105859.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6764..7738
                     /locus_tag="U4B29_000894"
     CDS             6764..7738
                     /locus_tag="U4B29_000894"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2105860.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7918..9063
                     /locus_tag="U4B29_000895"
     CDS             7918..9063
                     /locus_tag="U4B29_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2105861.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9200..9544
                     /locus_tag="U4B29_000896"
     CDS             9200..9544
                     /locus_tag="U4B29_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN2105862.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9815..11101
                     /gene="efmA"
                     /locus_tag="U4B29_000897"
     CDS             9815..11101
                     /gene="efmA"
                     /locus_tag="U4B29_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN2105863.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11239..11838)
                     /locus_tag="U4B29_000898"
     CDS             complement(11239..11838)
                     /locus_tag="U4B29_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN2105864.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12242..13363
                     /gene="mnmA"
                     /locus_tag="U4B29_000899"
     CDS             12242..13363
                     /gene="mnmA"
                     /locus_tag="U4B29_000899"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN2105865.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13609..15030
                     /gene="arcD"
                     /locus_tag="U4B29_000900"
     CDS             13609..15030
                     /gene="arcD"
                     /locus_tag="U4B29_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN2105866.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15044..16162
                     /locus_tag="U4B29_000901"
     CDS             15044..16162
                     /locus_tag="U4B29_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN2105867.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16222..17514)
                     /locus_tag="U4B29_000902"
     CDS             complement(16222..17514)
                     /locus_tag="U4B29_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2105868.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17646..18224
                     /locus_tag="U4B29_000903"
     CDS             17646..18224
                     /locus_tag="U4B29_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2105869.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18353..18730
                     /locus_tag="U4B29_000904"
     CDS             18353..18730
                     /locus_tag="U4B29_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN2105870.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18663..20735
                     /locus_tag="U4B29_000905"
     CDS             18663..20735
                     /locus_tag="U4B29_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2105871.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(20946..21893)
                     /locus_tag="U4B29_000906"
     CDS             complement(20946..21893)
                     /locus_tag="U4B29_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2105872.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22139..22603
                     /locus_tag="U4B29_000907"
     CDS             22139..22603
                     /locus_tag="U4B29_000907"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN2105873.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22665..23708)
                     /locus_tag="U4B29_000908"
     CDS             complement(22665..23708)
                     /locus_tag="U4B29_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN2105874.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24085..25050)
                     /locus_tag="U4B29_000909"
     CDS             complement(24085..25050)
                     /locus_tag="U4B29_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN2105875.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25284..26129
                     /locus_tag="U4B29_000910"
     CDS             25284..26129
                     /locus_tag="U4B29_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2105876.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26320..27507
                     /locus_tag="U4B29_000911"
     CDS             26320..27507
                     /locus_tag="U4B29_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2105877.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27513..27869
                     /locus_tag="U4B29_000912"
     CDS             27513..27869
                     /locus_tag="U4B29_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN2105878.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27871..28209
                     /locus_tag="U4B29_000913"
     CDS             27871..28209
                     /locus_tag="U4B29_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN2105879.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28285..28541
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28604..29638
                     /locus_tag="U4B29_000914"
     CDS             28604..29638
                     /locus_tag="U4B29_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2105880.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29631..30317
                     /locus_tag="U4B29_000915"
     CDS             29631..30317
                     /locus_tag="U4B29_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105881.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30341..31189
                     /locus_tag="U4B29_000916"
     CDS             30341..31189
                     /locus_tag="U4B29_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2105882.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31386..32159
                     /gene="sufC"
                     /locus_tag="U4B29_000917"
     CDS             31386..32159
                     /gene="sufC"
                     /locus_tag="U4B29_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN2105883.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32172..33458
                     /gene="sufD"
                     /locus_tag="U4B29_000918"
     CDS             32172..33458
                     /gene="sufD"
                     /locus_tag="U4B29_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN2105884.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33455..34690
                     /locus_tag="U4B29_000919"
     CDS             33455..34690
                     /locus_tag="U4B29_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2105885.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34677..35147
                     /locus_tag="U4B29_000920"
     CDS             34677..35147
                     /locus_tag="U4B29_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN2105886.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35152..36543
                     /gene="sufB"
                     /locus_tag="U4B29_000921"
     CDS             35152..36543
                     /gene="sufB"
                     /locus_tag="U4B29_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN2105887.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11948 a         6766 c         7848 g        10012 t
ORIGIN      
        1 atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga acaacagtct
       61 gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga ggaaatacca
      121 agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa agaatcagaa
      181 aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg ggttgttttc
      241 ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg cgtgcggctc
      301 tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat gacctattat
      361 tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga aatagaatct
      421 tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc agtaccaaaa
      481 aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg cagtcggatc
      541 gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag caaatatacg
      601 atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga tccgcaagct
      661 gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc agcagggttc
      721 ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa tctgtttccg
      781 gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac tggtttagag
      841 gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat gtatgactgg
      901 gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc agcaaggaat
      961 atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac agatcctcct
     1021 gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc tagtaatcat
     1081 tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt attgacaatt
     1141 aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat cactgcgaat
     1201 cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac tttcagtttt
     1261 acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta tgaaacaaaa
     1321 gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta tgtaacacca
     1381 gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac acaagcattg
     1441 agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc cttcgaaaga
     1501 ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca gttgattgca
     1561 gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg aaatgggatg
     1621 acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag tgcagctatg
     1681 caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca tgcttatcgg
     1741 aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga agcatggccc
     1801 gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg atttgaagat
     1861 gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga cggttgtatc
     1921 gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac aggttcgatc
     1981 ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc aggaattgag
     2041 ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg ccctcatgaa
     2101 ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa tagcggaaca
     2161 gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt ggctctttat
     2221 acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt ccgaacagag
     2281 aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca aatctaccac
     2341 tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct tggacatacc
     2401 atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca gcagcctgtt
     2461 acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa accggggaat
     2521 cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc tatttttgac
     2581 ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga accaaatcag
     2641 acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg tgtggcggag
     2701 aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc aactttcaca
     2761 ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa gatccaaatc
     2821 gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc agcaccttca
     2881 aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg ggaaaaatgg
     2941 tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga aactgccgat
     3001 gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc accaaatgga
     3061 aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga aaaaaatggc
     3121 actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt agctggagga
     3181 attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata agtggaattt
     3241 ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga aatgagacca
     3301 atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct gcttttgatt
     3361 ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact aaacggtgtt
     3421 caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa ctatttttcc
     3481 gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga taaacggtgt
     3541 tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa agccattttc
     3601 acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta atacatggta
     3661 ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg agaagcgatt
     3721 ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct cttaccagat
     3781 tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac caaatgtttc
     3841 atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag gggcatggac
     3901 atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc aaatcgttgc
     3961 ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg cttccaatgc
     4021 acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc cgtttacttt
     4081 tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt tgttttgaaa
     4141 ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt caaataaagc
     4201 gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga ttccaccata
     4261 attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg ctgccggaaa
     4321 gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac tcatttccca
     4381 ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg tgagtttgct
     4441 gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg ttttgaactg
     4501 gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt tatttaattt
     4561 tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta gacgtttttt
     4621 ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag cttcgccaaa
     4681 atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg caaggtaata
     4741 cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag aatagattcc
     4801 gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg ttttaatgat
     4861 catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca aatgatcgaa
     4921 gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac ccgtgacaag
     4981 ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt gcggattata
     5041 cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa tcaatttatc
     5101 aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc cggcaagctc
     5161 taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt tgggatggtc
     5221 tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa agaaggcgtt
     5281 cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg gtaatgtatc
     5341 caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg attcaacttt
     5401 ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat aatcgtttgc
     5461 cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt ctggatgggc
     5521 aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta aatcttggaa
     5581 accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt cttcattgac
     5641 tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc tccttttttg
     5701 tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat aaaaaattga
     5761 aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat gaaaaaaagg
     5821 aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt agataaaaaa
     5881 attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt gcttttttta
     5941 gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca tccggtagaa
     6001 tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg gaagctgatc
     6061 aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat tcccgttttg
     6121 atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt gccagaagaa
     6181 cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg tacttacgcg
     6241 gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct tccaaatgag
     6301 attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg tagccagatc
     6361 caaccactag gacttcctac gattcctttt caggttagta cagggatttc tagtttttta
     6421 aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg gcagcgagct
     6481 acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt acttggcagt
     6541 acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac aggagaacgt
     6601 tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc cagttggagt
     6661 aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc ttggaaaaat
     6721 atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta ataagtatca
     6781 ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg aaaaaatcgc
     6841 gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg atggcgagat
     6901 ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc aatcaacgaa
     6961 tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa tgaagcgagc
     7021 aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc aagaccgcac
     7081 ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg aagaagcagg
     7141 ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat tctttgatat
     7201 tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc aattaggtct
     7261 gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc gtgcacgtag
     7321 tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg atgcttcaac
     7381 tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg tagatgatat
     7441 cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa acggcgcaaa
     7501 agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa gtattttaga
     7561 caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag aaaatcgtca
     7621 tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg tcaaacgcat
     7681 ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat tcgaataaac
     7741 aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc tgcgacaaga
     7801 cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa aaatgagctc
     7861 ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt ggaagggatg
     7921 aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca tccacaagta
     7981 attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag catacatgga
     8041 tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc agacagtctg
     8101 caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga taatacagct
     8161 atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac tacagctatc
     8221 gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt tgacgttaca
     8281 tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa agcgttacga
     8341 gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa tttgatgcca
     8401 atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac agatgcggta
     8461 caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt attaagtatc
     8521 tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg agacggagta
     8581 aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc aggtacagaa
     8641 aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc tgaagaacgt
     8701 gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac attagaagca
     8761 aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca tgtgttgaat
     8821 cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct taatggggtc
     8881 gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca cgtattagaa
     8941 gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag ctttggtttg
     9001 ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc tgtcaaaaaa
     9061 taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc attttttagg
     9121 gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa gagaaactaa
     9181 ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg gcgcaccaat
     9241 tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg gatttacaga
     9301 aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc aaagcaaaga
     9361 aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa tgtcgatcac
     9421 tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga aaatgagcca
     9481 agataaattt tattttctga tggatgggat gattagccgt gggttattcg aaaaagtcga
     9541 atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta gatgactaac
     9601 tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca catgttgtta
     9661 tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg aaacgattgt
     9721 tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg ttgtgcaaac
     9781 gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt cggtcgtttt
     9841 aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt caggaatcac
     9901 tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg gttcggcgac
     9961 aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa gcccatttgt
    10021 aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg atattattgt
    10081 agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct tcccgatttg
    10141 gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa tgccaacgat
    10201 ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga atggacagtt
    10261 aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg ctttgttttc
    10321 agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt ttggtgtcgg
    10381 cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag ttccgcttac
    10441 tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag ggttatggta
    10501 cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca gtctgtatcc
    10561 attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag tggaagtagt
    10621 ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa catggaaaga
    10681 ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg gtgcaagcgg
    10741 attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg caggagcagg
    10801 ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga gttatgaatc
    10861 gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg caggtccaat
    10921 tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa tgttcttgtt
    10981 ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag ctgcccgaaa
    11041 ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa aagacgaata
    11101 gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc ctaatgttcc
    11161 tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt gtttaatact
    11221 tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat agatttcttt
    11281 gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga gttctctttc
    11341 cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat gatggatatg
    11401 tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat gagtcaagta
    11461 atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa atttgggact
    11521 tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc gactgtcatg
    11581 gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca aaatttctgc
    11641 atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa catggtcggt
    11701 ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat ccacatttgc
    11761 aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa agtgcatatc
    11821 actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa acaaaatcac
    11881 ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg tgcagcttta
    11941 agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta ttccttattg
    12001 ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc tcaaaagatg
    12061 agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag aacagtattt
    12121 ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat gtaaaatact
    12181 gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg atggtgatat
    12241 catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg tcgactcatc
    12301 tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat tcatgaaaaa
    12361 ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata aggatgtagc
    12421 gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa aagaatattg
    12481 ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac ctaacccaga
    12541 cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa tggatcttgg
    12601 cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa acggcgtggt
    12661 ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta gccaattatc
    12721 acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat cagaagtacg
    12781 ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa cgggaatctg
    12841 ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag caaaaaaagg
    12901 aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga tgtattatac
    12961 gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag aaccctggtt
    13021 tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt tccatcatcc
    13081 tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga atgaaccaat
    13141 gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg ttcctgtgac
    13201 agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg tacgtgcaat
    13261 cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg gtggtttgat
    13321 cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat taaatgaatc
    13381 aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag ctgttttttt
    13441 gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa agttcataat
    13501 atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg
    13561 aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa
    13621 tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc aatcggaagc
    13681 ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc agcgatcctt
    13741 tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa tcatttagga
    13801 gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc atttggtaaa
    13861 ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg aaatgttgct
    13921 tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa aggtggacaa
    13981 aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt attagtaaac
    14041 aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa attagtgcca
    14101 ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt cactgctgat
    14161 ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg aacgatctgg
    14221 ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat cgaaggcgca
    14281 agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc gatcattggt
    14341 ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg cgtcttatct
    14401 caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa aggaatcgtg
    14461 ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat tggttcttgg
    14521 ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga cggtctgttg
    14581 ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt agttgttact
    14641 gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc ctatcaattt
    14701 gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg gatctatcaa
    14761 gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg gattgctgta
    14821 gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt atgtagtatt
    14881 gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt
    14941 acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct ttcaatcggg
    15001 ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa tcgcaaattt
    15061 cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata ttgcacaaag
    15121 ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta gcgtaagtaa
    15181 atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag gatcagatgt
    15241 tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca tcttacaaac
    15301 gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag caggagacca
    15361 tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat ctttaggtgc
    15421 cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt tagaacagct
    15481 agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa acaatcctac
    15541 aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc gtacagtaga
    15601 tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg aattcttgtc
    15661 gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa cctactccat
    15721 tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag tttttcgaaa
    15781 gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc
    15841 attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag agaaccttgt
    15901 gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt
    15961 ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt gtatcaattt
    16021 attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac cgaagcatgc
    16081 ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc tcttatctga
    16141 gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga caactgttca
    16201 agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg aagctatgga
    16261 gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg
    16321 tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc cgcgataacc
    16381 aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag ttgcttggaa
    16441 agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa
    16501 tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac gtaaggcagc
    16561 taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg caaaacccaa
    16621 accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa atcttggagc
    16681 ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca acgtaacatt
    16741 cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa tggaattgtt
    16801 ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa
    16861 gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga caaatttttc
    16921 atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg taatgaaaaa
    16981 ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc caagtaatgt
    17041 tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt cttttggcgt
    17101 cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc cttgaagcag
    17161 acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa tgacgattgc
    17221 cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg ctaattttcc
    17281 ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca atccgctcca
    17341 aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg acataaaagg
    17401 catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga caaacaagtt
    17461 ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag ccaattagac
    17521 tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat atgtagttta
    17581 tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag tgaaggattt
    17641 attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg
    17701 actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt cagcatttag
    17761 ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat attcgtgatg
    17821 tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg cgacgggctc
    17881 ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat cgatcctttg
    17941 ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc aatgaagctg
    18001 ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt gaggcttttc
    18061 aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg atccacggtt
    18121 atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat ccagaagaca
    18181 gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc agattcattt
    18241 gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc ttgcaacttt
    18301 cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga gtatgtatca
    18361 agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact gggaagaaga
    18421 tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca
    18481 atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact ttctcagcgc
    18541 cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt tacagcaata
    18601 caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca gaaaggaatt
    18661 ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc
    18721 ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc gttacttggg
    18781 cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa tggcagagct
    18841 ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca cttcctaaaa
    18901 caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc tcagaagcag
    18961 tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc ttagagtatg
    19021 tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat aaagcaatca
    19081 aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat gaatctgctc
    19141 ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc ttaactgtga
    19201 agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca ttagtgagag
    19261 accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc acgttgcaag
    19321 ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag
    19381 aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca gaagtccaag
    19441 aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc caaagagaag
    19501 ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa agtttgtatg
    19561 agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca aatgacatga
    19621 aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt
    19681 atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt aaagtgaccg
    19741 atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta agtaatgaag
    19801 agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct caaccccata
    19861 aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt tttcggcaaa
    19921 atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa acagtcaaat
    19981 gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc
    20041 ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc
    20101 cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg gaacgtaccc
    20161 ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta aatccctctt
    20221 tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt gctgcaggaa
    20281 agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg
    20341 agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag aaatgcccag
    20401 aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat gtctgcacca
    20461 attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat cgatgcccgc
    20521 aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat
    20581 acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa atgaccaaac
    20641 atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa gaaagcccat
    20701 tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag gttgtgatag
    20761 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    20821 aaatttcggc ttatttccga agaagctact tctggaacac catttattcg gaaaaaagga
    20881 ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac ttcatttgac
    20941 gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc tcttatgata
    21001 taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc gatgattcca
    21061 agacataaca attggattcc accaatgaac aggaagatcg agaccatgga aggccatcct
    21121 gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc aataccagaa
    21181 ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc cgagaagttc
    21241 acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt ttcgccagcc
    21301 acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca gctaaataat
    21361 ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat ttgacgagtc
    21421 atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt attgatcaac
    21481 tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc
    21541 cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc aggaagcagt
    21601 tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt agaagcttgc
    21661 aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac aaagcggaca
    21721 taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt tgatccgtcg
    21781 tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa
    21841 agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt catcgtttct
    21901 atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa ccttaaataa
    21961 cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt
    22021 tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat tagttggcaa
    22081 gtagttttgg atagagaaaa aggaggaagt tccagtctat tgactaggag caaacaaaat
    22141 gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg gattgactgc
    22201 tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcgaattt taacgcgcac
    22261 acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg ttcctttatc
    22321 tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta
    22381 tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga aagctgtgcg
    22441 agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt gtcatcgagt
    22501 gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg gggttgaaat
    22561 caaacgccaa ttgttgttgt tagaaggata tctgcaaaat taaaaacagt tgtgattcgg
    22621 acattgttcc atgcgagaat cacaactgtt ttttcatgtt cattctaaac tttgatcagc
    22681 caaataaata gtaaagccat cactgtttgg agaagcccga cagctaatcc ataagataag
    22741 aagcgtgtac cttctttcat gaattttgca aagtctaaac gcagaccaat tgccgctaaa
    22801 gcagtgatct caaaccagct gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa
    22861 gaaaacaagc tgttgaccaa acaaaagatc acaaacccta atacatacca aggaagcggt
    22921 aattttcgtt ttttctgcgt atcagttggc gtttcaaaca gagctggttg tttcttgtgc
    22981 atgaattttt caaacagata gacaacaaca acgagcatca taatacgtag aatcttaaat
    23041 agcatggcga attcgacaac agaattgtca agtaagcttg ccccagcgac tacttgacca
    23101 acagattgga gtgtaccacc caaaagtgca cttttttcaa tgagatttcc accaaacaac
    23161 tgtaatccaa gaaaagggag agtcaacatc atcacagtac ccagtagatt gactagtgtg
    23221 atgatctgtc ctttttcttc ctctttcgca tggatggaag gggcgattga agcaatagca
    23281 gaagaaccac aaacagcgtt tcctccagcc atcatcaaag aagaagtatc gctgaattgc
    23341 agttttttac caatcaagta agcaccgatg atagtgactg acatttgcag taacacaaaa
    23401 acaattcctt ttacaccaat ttgagcaatc gtttgagctg tgacagttgc acctagtaaa
    23461 aaaacagaga attctaatag tttgctttca gcaaattttg tgccgattgc tagaacaggc
    23521 tgtttaaaaa aagtatttcc taataaaata cccaataaaa tagcaatagt cgctccacct
    23581 aactgtggca gataaatagc aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt
    23641 aaaccgggta agatttcgcg gaccgctgaa tcctttacag attttattgg atgtgacaat
    23701 gatttcattt gatcggttcc tttcgtcaat aaatactaaa agcatactcg aacatagtag
    23761 cactaaacca gtattttgac aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc
    23821 ttgtttcgaa taaacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca
    23881 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttcttt
    23941 tacgacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccagt
    24001 acaaaaacat gaacagctgt ttttgtttgg ccgttcttat tacttgatgc agaacagctt
    24061 tttcacaacc tattgaatcg attattaagc gattttttta gaaacaaatt ttccatgttt
    24121 gctatgttct ttaattaaga aacgtacgta atgagcaaga acgtagaaga caactacact
    24181 tgcaaacaga cattccgcaa cttttaagaa atccaatacg ctactataga tacctaaact
    24241 gttgaagtaa tgaacagtag taaatatagc tgttgcagag ataacaagcg ggaatgtaaa
    24301 ggctgcatag cttggataaa aacgaagctt gaacaaacgt ggaagcatag acagcacaac
    24361 gaaatacaat acttgagata aagcgaacaa tgctacgaca agataaaaat taggttcagc
    24421 aaagttttta agataacctg ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag
    24481 cggacgtgtc ggatgagcca tatttcttac gaaaaagaca cgtttgatga tgatcggcaa
    24541 taagaacgca tagcagatca atgaaatcca aaagaaaatt tttccaataa gtgggaagaa
    24601 gttcccggca gttacagtga tgaccccaaa tccaacgtaa atgataaacc agcttggata
    24661 aattgcttca attgaaagat cagctttgat aacatggtga taattgaaat aaaagacaag
    24721 gatcacttgg aagataacag cgcacagcca aatgaatttg acaattggtg caatactttc
    24781 atatgaaaca aaataagtac aaataaccat gagtgacatc gtaaaagttg gtgaaacgga
    24841 ggcgatgatt ggatcatgca acgtttgttt cgtgtgttcg aataagataa acaatttaac
    24901 aaaaatgaat atcataagaa tcatagagat accgccagtt agattgccta aagcaacatg
    24961 gtgatagtct ttaaacagat ttccaaggga agctaaccca aggatcaacc cgcagatcgg
    25021 gatcggaata attttcaaaa agttcttcat atataatatt ctccttttta tttaatagat
    25081 tttagtacca agatgaacga atacattgta aaatgaatag gaatgtatga ttacgagcaa
    25141 gcagacagct gattgtcttc atctaagtta ctagttggat cagcaactgt tttgttgtac
    25201 atttctatta tagatggttt tttgtatatt taaatcaaat agtttttata gtaattatca
    25261 taaaaagtta tggaagtggt gccatgttta agttattaac gacttttcga gcagtatatg
    25321 aaacaagaaa tttttccaga gcagcagaag tactgttttt atctcaacca gcagtctcga
    25381 atcaaatcaa acagttggag cgggaattga atatcgatct ttttcaacgt aacggaagaa
    25441 aagagatgct tccaactaag caagccgact tattatatga acggacactg gttttgttag
    25501 aagaatggga agaaactcag caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc
    25561 ggatcgttgc ttcccacacc tttgctgtct atcttttgcc tcagttgatg aaacatctga
    25621 tcaaaaggtt tcctaaagtg cattttgaga tcgaaatcac gaattctcat gaatcattag
    25681 aacaagtagc gaaacatgaa tccgattttg gttttatcga aaagccatta gagacttccg
    25741 gcgtacaaag atactctttg atggaagacc agctagtcat cgctggggac ccaaaaagca
    25801 aattgtggct tgtaagagaa gcaaactctg gtgtttttca ttacacgcag cgttattttg
    25861 aagagatgaa tatccaagat aagaagatga tcgtgaaaag taacgatgta attgttgcgt
    25921 tgctgaagga aggaatcggt cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc
    25981 caatgactcc gttgaatttc catcgatatt tttatttcat ccaacgaacc aatctggttt
    26041 ctgatcagct gctagagatt gccgaagaga tccttcgata caatgaaagc acagaaactt
    26101 ctcacagcga ggagaatctc agctggtaga taattgcatg tatcaaaaaa agttctatga
    26161 tacactaaag ataagcagta ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg
    26221 aaagttaatt tttcccaaat gtctatggaa agactttcca ttatctaact ggaccgctat
    26281 aataatcagc agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct
    26341 tcgaacgata atcgtattga ttatggcgtg attttaccag tctttctgtt atgtttgatt
    26401 ggtctgctct cattatatgt agcacttagc catgatccga acaatccagc agtcttgaaa
    26461 ggcgtaggga tgcaagttgt ctggtattta gttggagcgg cagcaatcgt tgtcatcatg
    26521 catttgaatt ccaaatggat ctggaaactg actccttacc tttatggttt ggggttagtg
    26581 gtaatgggac tcttgctcaa attctatgat cagaatttag ctgcttcaac agggtctaaa
    26641 aactggtttc gattcggtgc gtttactttc cagccagcag agttgatgaa gattgcgtac
    26701 atcttgatga tggcactgat cgtaactaaa cataatacgc aagtaaaaga acggacgatg
    26761 aaatcggact tttggctgat tgggaagctc ttgatcgtga caataccggt tttagcattg
    26821 atcatggccc aagatgactt tggaacgatg cttgtctttc ttgcgatctt tggcgggatt
    26881 ttcctgatgt ctggaatctc ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc
    26941 gttggagcag gaacgatctt cttggttacg actgaaggcg gaagagactt gctttataaa
    27001 gttggattca aatcttatca gtttgcacgt attgattcat ggctggaccc tttccatgat
    27061 acgtcaggaa tgagttatca gccggcccaa ggacttttgg caattggtac cggtggcttg
    27121 tttggtaaag gattcaacgt aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt
    27181 tttaccgtta tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat tttcttgtat
    27241 tttattttaa tctatcgtat gattcgggtg tgtttcgata cgaataatga attttacgcc
    27301 tatatcgcaa gcggtttgat catgatgctg ctgttccatg tctttgagaa tattggagca
    27361 aatatcggat tacttccttt aacagggatc ccattgcctt tcattagtca gggaggatcc
    27421 tcgattttag ggaacatgat cggtatcggg ttgatattat cgatgagata ccaaaatgaa
    27481 gcacctgcaa gatcttcaag gaggtaagaa aattgtacac cttttactgg tacccgaaat
    27541 gttcaacatg caaaaaagcc aaagcgtggt tagaccagca tggcgttagt tatcaaaccg
    27601 ttgatatgat caaagaagta ccgaaggcct caacgattga gacttggcta gaaacgagtc
    27661 aagtaccaat ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa cttggactga
    27721 aagatcaagt aggcgagttt acggcttcaa aagcgagcca agtcttatcg acagatggta
    27781 tgctgatcaa gcgaccactg atcttacgag aggaccagct tgttgcaatt ggattcaatg
    27841 aaaaaattta tgaaggagaa ctgatataaa atggcaggca agcatttgaa gaaaaaggac
    27901 aacctttgga tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa
    27961 gaagaacttg gagacatcac gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa
    28021 gatgaacctc tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc cagcccgcta
    28081 acgggtacag tttcttctgt taatgataaa atcggtgaag atatcaatat cttgaatgat
    28141 tcagatgaaa tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa agaatttgac
    28201 gaattataaa atcagaaaat cacttgaaaa aagttgacac tgattcttcc agttgctata
    28261 atcataagca atcctatatg aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac
    28321 gtttgaagag agccctgttt gctgaaaatg ggtaacgaaa atgagatgga agatggtctt
    28381 agagcagagt aagcgagcgt aagcaaactt actacgggag tgcccgttac agcgcgacag
    28441 tatacggaaa gaagctaagt ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa
    28501 atcaaggtgg taacacggaa gttcaagctt tcgtcctttt gcttaaggca agagggcgaa
    28561 ggcttttttg tttgaaaaat tataagagaa agatgtgaat ggcatgtcat taatccaact
    28621 aacgaatgtc aaaaagacat tcaatgggaa aaacggaaac atccaagccg tcaatgacgt
    28681 ttcactaaac gttgaaaaag gggatattta tggaatcgtc ggctattctg gcgcaggaaa
    28741 aagtacatta gtccgtctcc tgaatggact tgagcttccc acgagcggcg aagtgatcgt
    28801 aaacgatcaa gatattacaa aattaaaaaa caaagaatta cgtacattcc gaaagaaaat
    28861 cggtatgatc ttccagcatt tcaacctgtt atggtcaaga actgtacttg aaaatatcca
    28921 gctgccgctg gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact
    28981 gttgcgttta gtcgggttag aaggacgcgg acaagcatat ccttcacaac tttccggcgg
    29041 acagaaacaa cgcgtcggta tcgcacgagc attggcaaat gatcctgaga tcctgctttg
    29101 tgatgaggct actagcgcat tggacccgca aacaacagaa gaagtattag acttattgtt
    29161 agcgatcaat aagaaattga atttgaccat cgtcttgatc acacatgaaa tgaatgtgat
    29221 ccgcaaaatc tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga
    29281 cgtattgact gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga
    29341 catcgaacca gaagctgatt cgacagaact attagctgaa ctcatcaaag aaaatccaag
    29401 cggccgtttg gcaactttga cctttagcga aagaaatgcc aacgagccag tcatttccca
    29461 agcgattcga cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa
    29521 agaaggttcc tttggttcat tgactgtctt gatgactggt gaaaccaaag aattagatca
    29581 agcagaagtg ttcatcaaag aacaaggagt aggaattgag gtgatccatc gtggataaaa
    29641 gcttttcaga aacatatcta gattttaaac aaatcaaccc ggaaacgatg aatcaggcta
    29701 tccttgatac actatatatg acattaatct caatggtctt agtgacgatc atcggattag
    29761 ttttgggatt gatcctttac tctattggac gcaaaaacaa accgtatgct cgaatcattt
    29821 atggaatcgt atcgatcatc agtaatattt tccggtcaac gccatttatg atcctgatgg
    29881 ttttgatcat tccatttact aaagcaatcg taggcactat gctaggtgcg caagcagcca
    29941 ttccagctct tgttttatcc gcagcaccat tttatgcccg tctagtagag attgcttttc
    30001 gtgaagtcag ccctggtgta ctggaagcag cagatgcaat gggagcaagt tactgggaaa
    30061 tcatctggaa ggtcttgata cctgaaagta ttcccgcatt gatttctgga ttaacagtta
    30121 caacgatctc gatgatcgga tttactgcga tggccggcgc gatcggcgca ggtggacttg
    30181 gaggtcttgc ttggcaggaa ggctaccagc gtggtaatct aacagtgaca ttcgttgcga
    30241 ctttaattat tttactaatc gtatttatcg ttcaaggaat cggtgatttc ttaactaaga
    30301 aaacagacaa aagataatag gatatagggg gatgtaggat atgaaaaaga aaatttttgg
    30361 atttgcagca gcgcttttgc tgactgtagg attagcagca tgcggtaaca acagcgacag
    30421 caaagattca gcaaataaag cagacgacac gacattgaaa gtcggcgctt caccgacacc
    30481 gcacgcagag attttggaac atgtgaaacc attattgaaa gatgaaggaa tcgatcttga
    30541 aatcgtcaaa ttcgatgact atgtattacc gaaccaatct ttggaagaag gcgacattga
    30601 tgccaattac ttccagcata ttccttattt gaacaaagaa atcaaagaaa aaggatatga
    30661 ctttgtcaat gccggtgctg tccacatcga accaatgggc ttgtactcta aaaaagtcaa
    30721 agatgtttcc gaattaaaag atggtgcaac aatcattact tcaaattctg aatcagactg
    30781 gggacgcatc atcacaattc tacaagatgc tggcttagtc aaagtcaaag atggagtaga
    30841 tctggaaaca gcaacattcg atgatattga agaaaaccct aagaacttga agttcaacca
    30901 tacgatcgac ccatcacttt tagcttctac gtaccaaaat gatgaaggtg acctagttgc
    30961 aatcaacgca aacttcgctt acggcgcagg gttgaatcca ttagaagatg ccgtattgct
    31021 tgaaaaggac aactctcctt acgtgaatat tattgcgact cgtaaaggtg atgaagacag
    31081 tgacaaaatc aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac
    31141 agacaaatgg ggcggctctg ttaaaccagt aaaggctgat gcaaaataag gtatggaaca
    31201 aaaaagatga gaaataagtc tgtaatggct tatttctcat cttttttata cctattctaa
    31261 tttagaaatc aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc
    31321 attgcgcttc attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat
    31381 aatgcatgtc tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc
    31441 ttaaaggcgt aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg
    31501 gcacaggaaa atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag
    31561 aaggcgaaat tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc
    31621 gacttggctt atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg
    31681 aattcatgcg tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg
    31741 catttttgaa aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg
    31801 aacgttatct gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat
    31861 tgttgatgtt agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg
    31921 atgctttgaa agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat
    31981 tgatcatcac ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca
    32041 tgatggaagg ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag
    32101 aaggctacgc tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag
    32161 ggggagcaag aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt
    32221 ctttaagtaa aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag
    32281 cagatgaact gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg
    32341 atgtgaaaga aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga
    32401 aagacaatcc tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg
    32461 aattagcaga aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag
    32521 aactagtcca agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg
    32581 cactccatgt agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga
    32641 tcgatgaacc actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca
    32701 agcatgtgtt gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga
    32761 caaccaaaga acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag
    32821 ctggttcaaa aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata
    32881 tgaatcgccg tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga
    32941 atgacggtca tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag
    33001 aagtcaaaat cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa
    33061 ccaataaagc ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag
    33121 gaacattgac ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc
    33181 aacaagaaag tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc
    33241 ttttgatcga tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc
    33301 cagaagaaat gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag
    33361 tgattcgagg attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag
    33421 aattagtcga agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa
    33481 tgattttcct atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc
    33541 agcgacgacc caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga
    33601 taacgccaat gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga
    33661 acaagcaaga gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt
    33721 tactcgtggt acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat
    33781 caaagcagga gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg
    33841 gcagcagttg gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg
    33901 cttcctggat atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat
    33961 tgctcatgta tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt
    34021 ccatgaaaac ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat
    34081 tgatgtccaa gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc
    34141 aacaggtatt ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga
    34201 atttggtggc gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc
    34261 ttggaaattc gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat
    34321 cgattatttg caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc
    34381 ttatgtgttg ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc
    34441 gaattttcgg acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc
    34501 gacagcttta gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct
    34561 attgaagtac cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac
    34621 gcaagacgcc gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg
    34681 gactatctaa attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc
    34741 atcatcgtgg aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg
    34801 gagatgtgat cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg
    34861 acggcagtgg ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg
    34921 gaaaatcaat agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa
    34981 aagaagtcaa agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat
    35041 ttcccgctcg gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa
    35101 acgatggtac cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta
    35161 ccagaattag aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca
    35221 ggagaaggct tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa
    35281 tggatgcttg aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca
    35341 tggggccccg acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt
    35401 gacaaacccg ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag
    35461 attggtatac cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca
    35521 gaagtcgtgt accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac
    35581 actgactctg ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta
    35641 ccgccaaccg acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt
    35701 atttacgtgc caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat
    35761 gcggaaaaca cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc
    35821 cactatgtcg aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc
    35881 gtagaaatct ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca
    35941 gacaatgtgt acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa
    36001 tggatcgacg gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat
    36061 ggaaaaggtg cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa
    36121 gatacgggtg caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa
    36181 tcaatttcaa aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac
    36241 agtgcgggtt cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa
    36301 tccgatacga tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc
    36361 aaagtctcaa aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa
    36421 tctgaagcaa ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca
    36481 atggaatatg cagtcgaact caatcgattg atcagttatg agatggaagg cagtgttggg
    36541 taaacctgtt ataggagcta gaacccttga tata
//
LOCUS       DASJTM010000020        36307 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJTM010000020 DASJTM010000000
VERSION     DASJTM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.020"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(163..2298)
                     /locus_tag="U4B29_000922"
     CDS             complement(163..2298)
                     /locus_tag="U4B29_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2105888.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2381..4036)
                     /locus_tag="U4B29_000923"
     CDS             complement(2381..4036)
                     /locus_tag="U4B29_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2105889.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4038..4352)
                     /locus_tag="U4B29_000924"
     CDS             complement(4038..4352)
                     /locus_tag="U4B29_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2105890.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4522..4782
                     /locus_tag="U4B29_000925"
     CDS             4522..4782
                     /locus_tag="U4B29_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN2105891.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4796..5110
                     /locus_tag="U4B29_000926"
     CDS             4796..5110
                     /locus_tag="U4B29_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN2105892.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5840..6394)
                     /locus_tag="U4B29_000927"
     CDS             complement(5840..6394)
                     /locus_tag="U4B29_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN2105893.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6520..8449)
                     /locus_tag="U4B29_000928"
                     /pseudo
     CDS             complement(6520..8449)
                     /locus_tag="U4B29_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(8755..9471)
                     /locus_tag="U4B29_000929"
     CDS             complement(8755..9471)
                     /locus_tag="U4B29_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2105894.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9472..11295)
                     /gene="glpO"
                     /locus_tag="U4B29_000930"
     CDS             complement(9472..11295)
                     /gene="glpO"
                     /locus_tag="U4B29_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN2105895.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11309..12805)
                     /gene="glpK"
                     /locus_tag="U4B29_000931"
     CDS             complement(11309..12805)
                     /gene="glpK"
                     /locus_tag="U4B29_000931"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN2105896.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(12941..14401)
                     /locus_tag="U4B29_000932"
     CDS             complement(12941..14401)
                     /locus_tag="U4B29_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2105897.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14391..15686)
                     /locus_tag="U4B29_000933"
     CDS             complement(14391..15686)
                     /locus_tag="U4B29_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2105898.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16018..16695)
                     /locus_tag="U4B29_000934"
     CDS             complement(16018..16695)
                     /locus_tag="U4B29_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN2105899.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16725..17816)
                     /locus_tag="U4B29_000935"
     CDS             complement(16725..17816)
                     /locus_tag="U4B29_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN2105900.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(17917..18351)
                     /locus_tag="U4B29_000936"
     CDS             complement(17917..18351)
                     /locus_tag="U4B29_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2105901.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(18935..20392)
                     /gene="ascB"
                     /locus_tag="U4B29_000937"
     CDS             complement(18935..20392)
                     /gene="ascB"
                     /locus_tag="U4B29_000937"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2105902.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20389..22284)
                     /locus_tag="U4B29_000938"
     CDS             complement(20389..22284)
                     /locus_tag="U4B29_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2105903.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22342..24117)
                     /locus_tag="U4B29_000939"
     CDS             complement(22342..24117)
                     /locus_tag="U4B29_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2105904.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24167..25663)
                     /locus_tag="U4B29_000940"
     CDS             complement(24167..25663)
                     /locus_tag="U4B29_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2105905.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25857..27479)
                     /locus_tag="U4B29_000941"
     CDS             complement(25857..27479)
                     /locus_tag="U4B29_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2105906.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(27807..29120)
                     /locus_tag="U4B29_000942"
     CDS             complement(27807..29120)
                     /locus_tag="U4B29_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2105907.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29365..29589)
                     /locus_tag="U4B29_000943"
     CDS             complement(29365..29589)
                     /locus_tag="U4B29_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105908.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29609..30031)
                     /locus_tag="U4B29_000944"
     CDS             complement(29609..30031)
                     /locus_tag="U4B29_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN2105909.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30173..30883)
                     /locus_tag="U4B29_000945"
     CDS             complement(30173..30883)
                     /locus_tag="U4B29_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105910.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(30901..31398)
                     /locus_tag="U4B29_000946"
     CDS             complement(30901..31398)
                     /locus_tag="U4B29_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105911.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31435..31887)
                     /locus_tag="U4B29_000947"
     CDS             complement(31435..31887)
                     /locus_tag="U4B29_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2105912.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32022..32627)
                     /locus_tag="U4B29_000948"
     CDS             complement(32022..32627)
                     /locus_tag="U4B29_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2105913.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33077..33697)
                     /locus_tag="U4B29_000949"
     CDS             complement(33077..33697)
                     /locus_tag="U4B29_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2105914.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33699..34568)
                     /locus_tag="U4B29_000950"
     CDS             complement(33699..34568)
                     /locus_tag="U4B29_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN2105915.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34591..34998)
                     /locus_tag="U4B29_000951"
     CDS             complement(34591..34998)
                     /locus_tag="U4B29_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2105916.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35017..36306)
                     /locus_tag="U4B29_000952"
     CDS             complement(35017..36306)
                     /locus_tag="U4B29_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2105917.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        10291 a         7793 c         6069 g        12154 t
ORIGIN      
        1 cgtttttttg ttacgacgta ggagtttacc atacatttca tttcttttca cataaagtgt
       61 tcctaaattt ttcctaagaa aaagaacaaa acatttctca tcagcaaatg aaaaatattt
      121 tgttcttttt atcagttgtt ctttttattt tttatctctt aattatgttg ctctttccaa
      181 tacgacaatg cttttcattc ctctagatat cgccgtgtac atttgtctgc gtcttctttg
      241 ctcatctcca tcgttttcaa atggatataa gattacatga tcaaattcca gtccttttgc
      301 tagactcaaa ggaatgattt ggcagttttc taatccatat gaattttttt taatcgattc
      361 ttgtagcgtt tgggtttcct cttctgtttg ggtgataact gccgcttttt ctccttttag
      421 agaaaccaaa atctcagcta gtattttcaa caattcttca gaggatcgaa aagagcggta
      481 ttcgggttct tttccttgcg gtcggatcga tacgatgtcg atcttctctt gatccacagc
      541 ataggttttg aaaagctctg tgattgctcc gctagaacgg taactatttc taagatcata
      601 ccgatggatc ggcagatggt agtcgtcgaa acaacgcata atatcagcaa aagtagtgga
      661 agaattaaag attgcctgat tctcgtcacc tactaaagta aagtgagttt ttggaaataa
      721 ttcgatcaat agacctaatt gtgccagggt ataatcttgt acttcatcaa tcaatagata
      781 gcgaaatgct ggtacattta ccttttcaac aagtaaatga cgaatagcta atataatgac
      841 tgcttcatca acagtacgtg tgggttgcca agcataagct gagccatatc gtttttcata
      901 aattctttca aacaactgat gctcatccac ccatgccatt tcttcgattt gtctggttat
      961 ttttcgatat tttttttgta aaagtttcct tgcataagct acaatacttt gttccgaatc
     1021 atcagaaatc aatttaccga ataattccaa ctgcctatct tctgtcaacg agagtacctg
     1081 attctgcaca gcaggacttt ttgcttgttt caaaagatga ttcttccatt cgctcatcag
     1141 tgcctttttc gttgcttgaa gcctatcgat caatttagga tgagcaggaa ctttctcata
     1201 tattttttcg atcgtttttt tagaaaggac tgtttttcca tttttagtaa gtccacggat
     1261 aaaattcgga tgatctaaaa acaaaggatc tgattgtttt agattatcga taaacttttt
     1321 gctacgcaaa cgttctgtct gctccgatac attttctccc gtgatacgtt tgaaataagc
     1381 ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc agctgtatga tggttagatt
     1441 cagtggattt ctttcaccta atgatggtag gacattcgaa atatagtcga taaaccgatg
     1501 attaggtgat agaatcagca agtcatcgga tgtcatcgta tctcgatata aatagagcag
     1561 atacgcaata cgctgcatga tagcagaggt tttaccactt cctgctacgc catttaccaa
     1621 gatattttga cttttcacat cccgaatgat tgtgttttgt tccgattgga tcgtagcagt
     1681 gatagccttc atttcattgg tctcatcttg gcctaagact tctaataata cgtcatcttg
     1741 aatagctaca gacgtatcaa aaaacttgac caaacgatct ctttccaaaa gaaattgtcg
     1801 tctttcttta ataaccgtat cgattttatt ttgacgaacc gtataagaag atggaccaat
     1861 catgttattg taaaaaagtt cggcaattgg tgaacgccaa tcataaacaa gattttcatc
     1921 tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat tcttttggct cattatccag
     1981 aaagtctaca acgacttttc caaagtaggg acttttcaat aaacgttcga tctttgctag
     2041 ctgattttct gcgtttttct gtttgatatt catctgatcg atttctctat ttttcatttc
     2101 aatcatcgaa aaagtatcca ggttatctaa gtaactgtca aaattcaatc gaagctcgcc
     2161 agccatttct ttcaacgact ggtttccttc tatttttgcc gtatccatcg attcccgcag
     2221 ttcgtttttt ttatcaatta gcttttgata cacgttcttc agatatgctt cttctaactg
     2281 tttttgtgtg ttgaccatac acattctcct ctttttttaa agtggtggaa ccgattagtt
     2341 tacctctttt aagagtcgaa tgcaagcttt ttaacgagta tcatttgtta ggatatatca
     2401 gttcttcagg tcggacagtt tgatataaag aaaatgcacc atcaaggttt tttacgtggt
     2461 agccagactg cttcaagatt ctttccgcta gatagctacg taatccgcta tgacagctga
     2521 cgatatattc ttgttggctg tctaattcat ttaatcggtc ccgaagttca ttcaaaggga
     2581 tatgtttggc ttccttgaat ttgccctttt ccaattcttc tgctgttcgc acatctaata
     2641 aaatttttcc ttttgctagt tctgtcggca attcatgcca ttgtacggtt tcactaagcc
     2701 cttctgcaat gttcattgct gcatatccta gcatgtttac tggatctttg gctgagccaa
     2761 atggcggtgc atacgtcaat tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga
     2821 ttgctgttgc taaaatatcg atccgtttat ccacaccttt tgccccaaca ccttgtgcac
     2881 cataaatctc gccagtctct ggatggaaga tcaacttcag taaaatatct gttgcttctg
     2941 gataatagct tgcatgatct tttcctgata catgaatgac ttcaaaagac agttcgtttt
     3001 ctttggctgt tctttcactt aaaccggtgg atgcggcagt cattccaaac gttcgtataa
     3061 ttgctgttcc aataccgcct tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt
     3121 gtctaccttg acgattggct ggtgaagcaa gagaaataag tgcatctttt ccagtgattt
     3181 gctgttttac aacgatagca tcccccaccg cataaatatc aggaatattc gtttgataac
     3241 gttcgtctac taggataccc ccgcgtatgc ctaattcgat tccagcggtc actgctagct
     3301 ggttatcagg acgaacacca atggataaaa taactaaatc agcagggatg gtacgcccat
     3361 catccagtcg caatcgtttc ccgtggtctt caaatccagc gacagcatga gaagtaatca
     3421 cttctactcc ttgatgagac aattcttttt ctataaaagc tgccatttct tcatccagtg
     3481 ttggaagaat atgtggtgct tgttcgatca ccatgacttc taaacctctt ctttttaggt
     3541 tttctgccat ttccaatccg atgaatcctg caccaacgat cacggcacgc tttggctgtt
     3601 tttctaatgc atgcatcact ttatcgatat ctggtacatt tctaatagaa aaaacattgt
     3661 cggcttctgt tatccctgga atcgatggaa caactggttt tgcaccagga gataaaacca
     3721 atgcatcgta ctgttcaatg tgtttttgac cctcatgtac gacttctacc gttttgtttt
     3781 cgggaaagat ggctgtcact tcatgatttg gacgcacatc taaacaaaaa cgagcggata
     3841 atgattctgg cgtttggaca agaagatttt cccgttcact gatttctccc gaaagataat
     3901 aaggcaagcc gcaatttgca aaagaaacat acggtccttt ttcaaaaaca acgatttctg
     3961 catcttccat taatcggcga agccgtgtag cagcagacat tccacctgct acacctccga
     4021 caataacgat tttcatctta tttccctcct actgttcgat caaaccattg attcatgcca
     4081 ccacgtacat tcactacatc ataaccagat ttctttaatt ctttagctgc ttgtttactc
     4141 ctcatccctg attgacaaat cacatagacg gtcttttctt tgtctccatc atatctgttt
     4201 atactttgta atggaacatt tttagctcct tttatatgcc cgccccggta ttctgcaggt
     4261 gttctaacat caagtaacgc tggattttcc aaaagctttt cctttaattc tgggactgtt
     4321 atcgatttta ttgattgaaa tagcaaattc attttctgca cctcatttac atttttataa
     4381 atacctcatg gggtatatta aacaggagct ttattaatat gtcaaccaga atgactcttc
     4441 atttttttca ctacttttta tatttgcata aaataccgat aaggggtatt atctaattat
     4501 aaaaatagag gagaatgtgt tatggagatt gataataaaa atatcctgaa ccgtttgaaa
     4561 cgtacagagg gacaaattcg tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg
     4621 gatgtcatca ctcaattaag cgctgttcgt tctagtattg atcgcgtaat gggcatgatc
     4681 gttgcagata atctgaaaaa ttgctttgag aaacctgata gtaatccaga agaacaagcg
     4741 gctaaactag aacaagcgat caagatgatt attaaaaagt aaaggaatga tttcaatgaa
     4801 agtcgttttc catattgatg aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa
     4861 cctgataaaa gcttctccag aaacgacgat tgtcgtatct gtaaatggca tcgcgattac
     4921 cggttatcta gactctgcca atgcagaatt cttagatttg caaggagtcg tttttcacgc
     4981 ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt tctttacctg aacaagtaat
     5041 cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa tcccaaggtt atgcttatat
     5101 caagccttag tcagtagagg ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat
     5161 ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc
     5221 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttgggct
     5281 tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcggt
     5341 ttgacctaag tattagagaa aaaaacggga aaatagctcc tcatattttg ccgttttttg
     5401 agctaatacc gaaggtcagc tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg
     5461 cttagtttcg agtattaaga aaaattttgt gaaaatggat ttctacattt ttgcataaat
     5521 ttggcttaat accgagaaac tggcttttga ataccgttta aggaagaggt tgtaaaatca
     5581 gcggtttgac ctgagtatta gagaaacaaa cggaaaaata gctgctcata ttttgccgtt
     5641 ttttgagcta ataccgaagg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
     5701 accatttaac tccgaatcac aagaaataat ttcaaaaaca acttctcctg tttttggttc
     5761 ggttggctta ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa aggaatgcga
     5821 cataggtcac accccttttt tattttcttt gaaacatctt tcggtactcg acagcataca
     5881 agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc acccacagcg
     5941 ttggtaattc tgggaaaagc agcaaaagga tcatgccaac atagatgaca gcagtcgcta
     6001 ctttgccata ccacttcgct ccacttaatt tgatattttt agatagcccg attaatccag
     6061 ctacaaacat aaagccttct tttatcaaga atagaccaaa caacagccaa agccacggtc
     6121 gataccatgc catagacaaa atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa
     6181 gcaccttccc taagtctgtt acctgattat atctccgagc aatgaccccg tctagataat
     6241 cagacaaacc tgagacaaca atgaccgctc ctgctatata aaatgactgt ctcagcacaa
     6301 aataaagata aatcggcagc aaaaaaatac ggaatatcga taaaagattc ggaatcgttt
     6361 gtatttcttt acgccagtct gtgcttaatt tcattttatc gccctacctc ctcagcactc
     6421 ttttcttttt attttagctt acacggggaa taaaaccaat gatttcactg tttttatttt
     6481 tctttacaga cactgatatt atgagctaat ttgagataat cacttgctgt tttcatcgtt
     6541 tactcttctt tcttcctgtc gtttgcgcat ttcttttact agtactttat gagctttttt
     6601 tgatcctcgc tctaggtcac gctgccgttt acgtgtttta taatcgataa acagactgtc
     6661 taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc accagacgaa cacgctgacg
     6721 aggcacgtgg attatttttt tgtctaaata tacatcaatc gtatcttcac ctatatcttc
     6781 ataaactaac gcttcttttt ggtattcatt gacaaatatc cgatcacctt ttgcaaagag
     6841 ctgacgttct tttttattct ccttcctatt tgtaactgct gtcttaggaa gaaattcttt
     6901 tttctgtgtc cgataggctt tttccgccaa gtaacgctct gcttgatgga tcaatttcat
     6961 cgacatatcc attttatgag caatccacaa agcttggctt tctcctgttt cttccaaccc
     7021 gcaactgata tttaggacga agtgcttccc gatcgaaagc catagcagcc ggcacaaaat
     7081 cttcatgatc tccggcgaat ttcttgattt ctccataatg ggtcgtggca atgatcaatg
     7141 ccccttgctc atacatagat tccatgattg cgatcgctag tgcagcccct tcatttggct
     7201 ctgttccact gccaatctca tctaaaagta ccaacgtatt cctttttatc tttttcaaaa
     7261 tagcagcgat gttttgcata tgaccagaaa atgtactcaa cgcattttct aaattttggt
     7321 ggtcaccgac atcgacgaaa atctcatcaa agacagcaag ctctgtccct tcttttgccg
     7381 gaacttgcat accgaacatc gccatcaaag tcaataaacc gactgtcttc aacacgactg
     7441 ttttgcctcc ggcatttgct ccggtgataa tcaagcctcg atagtcttta ccgatttcaa
     7501 catccaatgg taccgcatgc tcaggtaaaa atggatgtct gccttgcttg ataatgatat
     7561 gttccgattt attgattcta ggtgtgcttc cattgatttc tcggctaaat tttgcccgag
     7621 caaagatgat atctaacacc gtgatcgttt cgatcaataa atcgatcact tcttcgtttt
     7681 ctgcgatagc accagtcaat gccgccagga cttgatattc ttcactgatt tcttcagctt
     7741 ttaacaactg atagtgttca ttcaatttag aaatcacagt tggttcaata aaaaccgtcg
     7801 ttcttttatt tgattcatcg ataatcgttc catctatttt atttttatag ctggctttga
     7861 taggaatcgt atagtattcg cctttttgaa caatcattgc ttcttggatc atttctttgt
     7921 ttttcggatg acgcaagaat ttcagtaatt ttgactggat ttctttttcg attagttgta
     7981 attgtttgcg tactttacgc aaattacgtg acgcgtcgtc actgacttta ttatttttta
     8041 ttttctggtc aatcaactct tctacattta tcaaatcagg aagatgtttg ctataagcaa
     8101 acaacaaggg tgtctggtat tgattcttat caaaaaactt tgtgatcatc cgactgcttc
     8161 taagaaaatc cgcatattcg atcaaatcgg ttggctgcag aatcaaccct tttttgactt
     8221 gttctgtcag tgcatcgatc ctcggcaacc ccataaatgg aacatgctga ctgctttcta
     8281 agatcaagcg tgcttctctt gtttcttctt gacgatccaa gacagtttgt aggtttgtcg
     8341 agaccgtcat atcgctgatt cgttttttgc tatagtttcc aattgctctt gcttggattt
     8401 cctttttgat ccgatcaaat tgtaattgac taatcatttc gttgtccata ttctctcctt
     8461 ttcttgaaga gatacctgtc ccaaaataaa taagggccta gagataaata aaaggataga
     8521 acacagctta aaaaagtcat gtcctatcct tattagaata caaagatatt gttttcctga
     8581 caggtaccgt ttcataagca caccaaataa actttgttta ttggctattc gacttactga
     8641 ttagatacca gctacactca caaaacatac cccattcttc taggtcttat ttattttcca
     8701 cttaactata gcaagacaca ttttggttgt caaactttag tatgttttat agatttacac
     8761 cattaatgaa tagacaccta ccgcgataat tgctccgatc attggacctg cgattggtac
     8821 ccatgaatat gcccaatctg aagttccttt attagctatt ggcaataccg catgtgctaa
     8881 tcgaggacca aagtcccgag cagggttgat tgcatatcct gttggccctc ctaatgaaag
     8941 accgatggct aagatcaatc cacctacagc gaatacgttc gttccaggtg caaattcgtt
     9001 ttgtccaaaa gctagcaacc caagaactaa tacaaatgta ccaattaatt ctgtgatcac
     9061 atttgctgga taattccgta tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc
     9121 atctttcgtt gcattccaat gtggcaaata agaaagccaa acaagaatgg cccctgcaaa
     9181 agctcctaaa acttgcgcaa tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt
     9241 catcgccata gctagagaga ctgcaggatt caaatgagct gggcccatga atccagaaat
     9301 atagacagcc attgttacag cgagtcccca accaaatgcg atgacaatcc agccagaagc
     9361 ctgtgcttta cttttgttta aattcacagc cgcacacaca ccatctccaa gcaaaactag
     9421 gatcagtgtc ccaataaact cccccatgat ttgagtcata tcactgttca tttatttctc
     9481 cccctttagt tcccgcagat ctgattctgc aatcaatgct tctaaacgtt tagtctgttg
     9541 cgccttctcc tcttctgtcc attcaaaata tccagcaatt gcatcgatca caggttgctt
     9601 gatctcatca agctgatctc gttcaaacaa cagatgattt gtacgacgaa tgagataatc
     9661 gcctggggcc aaaaccattt cttcttctaa accataacgt aaccgagctg actcagcgag
     9721 actcaaacca ggataaggtg ccatttcttt tgctaattca aagatacgtc gggcattagt
     9781 cccgtaaaaa tcagcgatat aagtagcatc ttcttcctct aaaccggctg ctactccttc
     9841 ttttgctaat tctgtcactg tttcttctaa tttagttgga tcgaagtttc cacctgaaat
     9901 ctgatatttt ttagagtcga tttctttcgt ctctattccg tattcttctt ttaataattg
     9961 gcgtatcaat cgcaaagctc cttctgccat cttacggtaa tcggtgattt tcccaccaga
    10021 taaagtcagc aaaccatctg gttctctttc taaagaacta cctctggaaa tcgtagaagg
    10081 tgctttttca tcacggctgt tttccaaatg gttcaacacg tcttctactt cagcacgaga
    10141 aactttgttt tccttatatt cacttactgt atcgacaact ttattgaagc tcttgtctga
    10201 aatcgatcca ttatctccac cattgtaatc agaaccagaa ttaccaatca acagtggacg
    10261 aagtcctgcc cagcttgctt cgatatcatt caatgtgata tttgcttctg gatagcgatg
    10321 attgatcaca tccaatagat aatctacatc ttcttgtgtg actttcggat ccgtaaaatc
    10381 tccttggtaa tccgtatctg tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa
    10441 aaccatccgc ccatcttgct ttcctgtatc gaagtatgtg ggttgcggta caggcagttt
    10501 tttcgcatct acaactaaat ggatcccttt agttggacgc attttaggag agactggacg
    10561 cgtaaaattc aagtttcgta ctttatctac ccaaggacca ctcgtattga tcactaattt
    10621 tgctttgatt tcgatcactt catctgtcag caggtcacga gctttgacgc caacgatttg
    10681 atcgccatca tataaaaagc caaccgcttt cattttactt actagataag ccccatcttc
    10741 ggcagccttt ttgatattat caatcactaa acgggcatca ttgttgcgga aatctagata
    10801 gacacccgcg ccttttagcc cttctttttt caaaaacggt tctctttcca atacttcttc
    10861 tggtgtcagc gtataattct catatttggt tcctgtcaca tttgccagtt tgtcataaag
    10921 gtccattgct actttgaccg agaacatatt gaaggttgtt gctccttcgc cctcatagat
    10981 tggcaaaagc attggatctg gttttgggat gtgtggggca atactttgta cgactgcacg
    11041 ttcaccaact gtgtcagcta ctacttccac atcaaatgtc ttcagataac gaataccgcc
    11101 atgaacaagt ttggtcgagc gagaggaagt cccttctgca aaatcctgca tttcgatcaa
    11161 tcctgttttg attcctgatg ctgctgactg gactgccaca ccggcaccag tgattccgcc
    11221 accgatgatc aacagatcca attcttcttg ctgcattttt tgaatgctat cttgtcttgt
    11281 cttgtttgaa aacatatgga tttcctcctt attcaaatgc ttgggtagct tttactgctt
    11341 ttttccaacc gttgtaaagt ttttctcggc gctcttcctc catttgaact tcaaatagct
    11401 tgccagcttc ataaaattcg cggatctcgt ctgtatcttt ccaataaccg acagctaatc
    11461 ctgctaaaaa tgctgcccct agcgccgttg tttccaagtt ttctgctcgt tggatcggta
    11521 cattcaagat atctgtttgg aattgcatca gatattcatt gtttgctgca ccgccatcta
    11581 ctttcaatac aggaatcttg attccagtgt catcttgcat cgtatcgatg atatctctca
    11641 cttgataagc aatcgactgt aaagtagctt taatgatatc ttcttttgtc gtaccacgtg
    11701 tcaagccaaa catagcccct cgtgctgcct gatcccaata aggagcacct agcccaacga
    11761 atgcaggaac aacataaacc tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat
    11821 cgctagcttt ttgcaacatt tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca
    11881 caaaaatact tccttctaaa gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg
    11941 tcaataaatt atttttggat aattgcggtt tttcaccggt attcatgaca ataaatgaac
    12001 ctgtaccata agtattcttg accatgcctg gttcgaatgc catttgtccg aataatgcgg
    12061 cttgctggtc tcctgccatc cctgaaattg gtgcttcgct tccataaaaa tgataacctt
    12121 gcgtataacc ataaacttct gagttagaag tgacttttgg taacatcact cgaggaatat
    12181 ttagtaaatc taaaatctcc tgatcccaat ctagctgatg gatattgaac aacatcgtcc
    12241 gactggcatt cgagtaatca gtaacatgtg cttgtccatc ggtcagtttc catactaacc
    12301 aagaatcgat cgttccaaac atcaattcgc ctttttcagc tcgttcttgc gcgccttcta
    12361 catgatcaag aatccagcgt atcttcgttg ctgagaaata ggcatcgatc actaaccctg
    12421 ttttttcgtg gaaaaattct tgatagcctt cattttttaa gttatctgca attccagctg
    12481 actgtctgga ttgccaaaca actgcatgat agattggacg tcctgtatct ttttcccata
    12541 ttactgttgt ttcacgctgg ttcgttatgc caatcccagc aatctgattt ggtttgatcc
    12601 ctgattcgat gaacgctccg gcaataactg actgtacaga attccagatt tcatttgcat
    12661 catgttccac ccaactttct tgcgggaaat attgcgtgaa ctctttttga gaactgccga
    12721 tgtgtcttcc tttcttgtca aagataattg ctcgtgagct agttgtacct tggtcaatcg
    12781 ccatgatata cgttgattct gtcattgtga ttccctccat catatgcaaa cgctttcttt
    12841 aactactctt atcatactac tgttttaaaa aaagaaactg tcaaaatttt attctttttt
    12901 tctttttttg acagtttttt attatgaagc tattttttaa tcaattcttt tcttttttca
    12961 gcgtctctat tcttcttttc aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat
    13021 aatattcaca ctgttttttg tttctctggc aaaatgtgat caccaaatca tattctttac
    13081 cttcttcata acttccaatc tcaataccgc cgattcccct cagttctctg atcagatatt
    13141 gttgaaaagc aatacgataa atcggcagac tttctaaatc gattccaatc actactggtt
    13201 ttgcaatata aaaatcgatc atcaaaagaa cattggcata ctggatcatc aggtaactaa
    13261 gctcactatc ttcagctctt ttcctcggca attgctgcac cgctgtttcc tgtaatttcg
    13321 ccagtaactg tgttaaggaa tgcccgagca ttttctgctg ctgttccaaa agccgatcac
    13381 gctcatagac ttcgatcttt ccaacaaaga aaaaccattc attgttgatc tgggaaattt
    13441 ggtagcctac tgccttttct tctttgattt tcaaacgacg aggacgataa tgccataaca
    13501 tcgtttcccg aatgtaagta tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat
    13561 catactgata aaatatttct tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca
    13621 tcattgtttc gtatacattc agttctgctg ctgttcgact aagatgcatc acgatgattg
    13681 gatcgattgc ttgataaagc cggtccgact gatagagtaa tttcttttct ttcaaagttg
    13741 cgtacgtact tttttcattc aatagtcttt ttttcgtaat cccgagccag caagcaatct
    13801 gtgcttctgc tgatgccgta aacgttgttt ctaatacacg gtccaatcct agaatgaacg
    13861 gtcttttttc tggtgtattt tgcaaaaaaa gcggtcgctg atcttccggg atatattgga
    13921 atagttcata ataaaaatat cgtatctgta gctcttctcc gtataattga ccgtttttga
    13981 tttgtaatcc aaattcctct aagagctggt tcaattcttt gatctttcga aaaacagaag
    14041 actcactgat attaaacgct gtcgctaatc gaacgatgga tacttgctga tgttctaata
    14101 aatagacgag catcttaaac ttcaagcttt cctgcaataa aaacaataga attttttcca
    14161 aacttacatc ttcgtccaac acaagcagcg cctgatactc atttcgctgt acttctactt
    14221 ttttttccat cattcggcca atcaattcaa tatcttccaa atattgatca aacgaggctt
    14281 ttgacagttg gacatgctct ctcaaaccat tgatagaggc tgttccccct tgcagaacaa
    14341 ggtttcgcaa aatcaggatc tgtaactgct cttttctttc taataatgct tcaatcttca
    14401 taggcagtat tcgccacctt tgaccacacc atatctggca cacggtattc tggctggaaa
    14461 aatagctcat gttcttctaa ctgttcaaac gtccatcctt cttctactgc aattgtcagt
    14521 tggtcaagaa ggtggaacat ctcttttttg ctttggagct gtgctccaag aatctttttt
    14581 tcagaatcaa atagaaattt gagtttaact ttgctttgat ttattgctgt aagcttgtag
    14641 tctacgtatc ctgtttcaat tttttgggga taaaaaatgc tctcttcttc tgtcaaacct
    14701 acgcttccta agaaatagcc aaaccactga ttgccgactg gacgataggt tccctgatcc
    14761 tttgtctgtg agccactaat tgtcttgctg acatttcgtg ctgtacggac agcgttattt
    14821 actagagaaa catacatttt ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca
    14881 atcgcataaa cgtctttttg agacgtttgc aagtagtcat ctacccagat cgttccatca
    14941 tcattcaatg ttaattgttc ttcccacatc tgattgtcgg gacgagaatg attagccagc
    15001 aaaacaagat catagttctc ctttcttttt tccgtctcta caatagcggt ttcattttcg
    15061 gtcaaagaca caacttgttc ttctaaaaaa agtgaaagcc cttgattttt catttccttt
    15121 actaacggtt ctgtcatttc aggatctaga taacgaaaaa gaattgactt tcggctttcg
    15181 taaagatgga tttgtttttt tcttgatgcc aacccttcag ctatttcgat tcctgcttgt
    15241 cctgcaccaa taattgcaat tttctttgca ttcatgatct tttgctgaag agccgttacg
    15301 ttttgtatct ttttaactgg gtgaataagc gttgattctg cagaaatatt tcgaaacttt
    15361 tgatttgaac cagtcgctag aatcaaacga tcaaaatcca atttttttcc tcctgctaaa
    15421 acgacttttt tttctttcaa tcctattact gtcttttctg tgtaaacatg gattcgatag
    15481 cggtcagtca gttcttcttt cgtgatccaa gaaagttgtt tttcactggt aacctttcct
    15541 tttaatatca aattgaaccc gctgggaatc catcctaact tcgattgctt ttcaatcaaa
    15601 tatatttcac ttgttggcat caattggcga agataaatag ccgcatgaat ccctgcaaac
    15661 gacccgccga caattacaat gttcatctgc attcctcttt cttataaggt gttctactgc
    15721 taacttctca atcagaaaac acagtattca taactcgctt tcaaccttat gatacatagc
    15781 gatgcagcag aatcaaccct ctccatcata ccgtgttcta ggtctgcttc tcaataatgt
    15841 actagtggac ctaattgaag agtatcattt ctgaagagat gtttcaaaaa atgtactctt
    15901 ataaaagaaa aaaactcgcc ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg
    15961 aaaagacgag ttttagcgtg tgtaatgtaa tagttagaat agacacatca tttattttta
    16021 attctttaat gcttcaacaa ctgcttcgcc taattgatga gcagattgag gattttgccc
    16081 tgtaagaagc cgcccatcaa tttgaaccac ttctgaaaaa gctgctccag actgatagtt
    16141 tgctcctttt tctttcaaag catcttctgc taagaatggt acttcattcg ttgtaccatt
    16201 tgcttcctct tcttcattac tgaacccagt cactgtacgt ccagctaaaa tagattttcc
    16261 agactcatct gtaactggca acaatcctac aacaccgtga caaacagctg agaccagccc
    16321 ttcatttcga tagatttgtg aagcgacttt cccaagatct attgcttctg gaaaatccca
    16381 cattgttccg tggccaccag cataataaac cgctgcataa tcatttgcat ccacatcatc
    16441 aggacgtaag gtatttccca aggcccgatt tctgaaatct tcatcttcat aataggccca
    16501 atcgatttca tccatattca aaacactttg cggttctaaa ggtacatatc cgcctttagg
    16561 actgacaaaa tctgcatcga tcccattttt tactaataca tcataaaaat gagtcagttc
    16621 acttaaccac aaacctgtag gacgctcgat cttttcgtac ttttctacat ttgttaatac
    16681 gatcaatact ttcattttcc catcctcttt ctataaaaat tgcgttattg ataacgtgct
    16741 ttcaagaaat tatataatgg gcttggcaaa taaagctctt tatatgcttt ttcaaaaggt
    16801 ttcgttacaa attcatcctc acggttttcc aataatttct ctgcccagtc aggatcgacg
    16861 atcatttgct gtccaattgc tgcaagttct gcatatttca atacctgctc tacatcttcg
    16921 cgattgcgga tcccaccgac accaactaaa gggatctttc cttgaatcgt gttatggacc
    16981 acttcaagaa tcgatttatc gttgtattta tcagaacgag ctattcgatc atacatattt
    17041 aatgaaacat gcagataatc tagttttgac tcacgtaatc gttcaagaag ccagatcgtg
    17101 tcttcaaaac ggatacctgg tgtttcatat tcttctggtg agaaacgata ccctacgatg
    17161 aatggttttt tagcatattg ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata
    17221 aaacgataac gattttccaa gctaccgccc cattgatcct ctcgtctatt cgaatgcgga
    17281 gagaaaaatt gttggatcaa atacgtattt gctccatgca actcgattcc atcaaatcct
    17341 gattcaatcg ctctacgtgt ggctttgcca aatgcttgga ttgtttcaac gatttcttct
    17401 tcactcattg ctcgtggtgt ttccgcatca ggacgttttg ctgcaactgc acttgcagat
    17461 acaggctgtt ctccagataa agttgctcga cttgtcattc gtccaccgtg gaaaatctga
    17521 ataatcgctt ttgcgccttc tccttggatt gctttcgcta gctcacttaa tcgaggaatc
    17581 atttcatcat gagcgatgct gagttcacct ggccagcctt ttccgatatc ttggacattt
    17641 gcagcaccag taataacagc accaagacct ttagaacgtt gtttataata agtgatctca
    17701 tcttcggtga tcactccatt gaaaaagctg agttgtgtcg tcataggcga cagcatcaat
    17761 cggttcttca attgaactcc tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc
    17821 attcctccca taattcgctc tgattaacct atcgagaatc atctatcatt aaaccactcg
    17881 attagttgta catgcaacta atttgcttaa aaaattttat tttttttgta acttatctgt
    17941 tgcatcattc attgtctggg ctaattcttt acttgcttca tttcccaaaa ctgctttgta
    18001 tgactgatga agttcttccc agctaccatc gacttcctta gcaaattggc gtccttgttc
    18061 tgtaagataa actaatgaca agcgaccttc gctaactgtt ttgacgtatc ctttatattc
    18121 taatttttca acaaaacgag caatcgttga tggggcggaa tagagcattt gtgcaatatc
    18181 tttttgtggt attccatctt tttcttttaa tatcaataaa ataaaaccat aagttggtgc
    18241 cagacctgtc cttctaaacg cttcatcagc taatttattc aacatgcgat ctagcttttt
    18301 cacgctaaag aaaagacatt catctaacgc tttttcaatt ggcttttcca tttcatcccc
    18361 tccattggcg tacatatcat agtatgattt ttattttttg tcaaaaaaca cggaataatg
    18421 tccgcctcct ctgtctagat ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt
    18481 gttttcgaca aaactccttc tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag
    18541 ttttgagtat gaagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
    18601 ttaataccga aaaactggct tttgaacacc gtttatatca gaggttgtga aaaagccgtc
    18661 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttg tcgttttttg
    18721 agctaatgcc gatggtcagc tgattgtaca ccgtttattg agaggtcgtg agcctgccgt
    18781 tgaactccga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt atgactatgg
    18841 tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga
    18901 caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca tttgattgga
    18961 tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg ccattccctt
    19021 cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa gtagaagcgc
    19081 tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca tctttgatag
    19141 cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg
    19201 ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg aacagtggtt
    19261 tttgatacct atcatataat tgattcaaag caattctcaa acccttagga tcaatctgcc
    19321 agccccattc acttgatgga agatacggat ttttaattcc tccagttatt aagtttcctt
    19381 ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag tagctaaaag
    19441 aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct atttttaagt
    19501 tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct cttgcttgaa
    19561 catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata tcatccggat
    19621 cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt gaacctttga
    19681 taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg tgtgcacctt
    19741 ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg ctaacaaaag
    19801 ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact ttatttttat
    19861 agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc
    19921 atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga gaaacgactg
    19981 gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat aatcccgctt
    20041 cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata gacatccgat
    20101 agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga tgatagaaat
    20161 catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc gcttttactt
    20221 gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa tcgctgtcga
    20281 atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca ccttcacatt
    20341 gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc atatcgatcc
    20401 ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa tgatattttt
    20461 gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct ctttgattaa
    20521 ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc catcagatac
    20581 tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat ggatcagtat
    20641 ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac
    20701 ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa acccttctcc
    20761 taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta tttcacctgc
    20821 tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat ccttgtagcc
    20881 gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag caactagcca
    20941 aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag caactgccaa
    21001 agcaccggct cctgcggcac cagctactcc accaaatata gctccacata tacaagcaat
    21061 gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag tgattccaaa
    21121 taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt tcgttttcaa
    21181 aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg ctggacctag
    21241 cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc cccaatgtaa
    21301 cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata aaacagggct
    21361 aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat ctgcaattgg
    21421 acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg gtgtcaataa
    21481 atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag aaagaaccca
    21541 tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca cgggcatccc
    21601 tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat aaacgagagc
    21661 tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag cagtatatgc
    21721 caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa tgatataagt
    21781 tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc ctttgatcaa
    21841 accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa
    21901 agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat catcttctag
    21961 tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta ctacgacttg
    22021 aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta ccttctcctt
    22081 atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt
    22141 gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc
    22201 catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa gataagtata
    22261 gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga acgaaactgt
    22321 acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa agaaaacaag
    22381 tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct tcctttgctg
    22441 atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt ggcttttgat
    22501 aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag aactaggaga
    22561 gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt cttctttgta
    22621 gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct tttcacgcaa
    22681 aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat aaatcaaatc
    22741 atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt ccgttatttt
    22801 gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga tccacatgtt
    22861 catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct ggttactaac
    22921 aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa
    22981 ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat
    23041 taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat tcatggtatt
    23101 atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa attctgtttc
    23161 atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct caaatgctct
    23221 tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat ttaactctaa
    23281 tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta gttctgatga
    23341 taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt ttgttttttc
    23401 taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc
    23461 ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct tttggacagt
    23521 cggcatgata ttggaaaaaa ttgtactgat ttgttgagga aaccttgcgt gttggatcac
    23581 cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag caatgatttt
    23641 tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga tccaacgcaa
    23701 aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt ctgctatctt
    23761 ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg aagctcggaa
    23821 atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct gtttagtttc
    23881 agattcaatt gattctaaaa aataacctga acgtttattt gactggataa tgattttctt
    23941 ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt tggtacctaa
    24001 agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata ctacaagaat
    24061 ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta taaacataca
    24121 ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat ttgaagaaag
    24181 atctatattc aaggttttta ccattagact ggataatttg tttataatat tcaaaactat
    24241 cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc
    24301 catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata
    24361 agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt
    24421 ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta
    24481 gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc aattcattca
    24541 tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct
    24601 tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt
    24661 ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca
    24721 agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt
    24781 aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt
    24841 taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg
    24901 cagaaaagct acacataata cccatcttag catttggatc gattttcttt aataattgag
    24961 tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta
    25021 gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat
    25081 aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt
    25141 ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt
    25201 gccacccccc atactctcct accaatgcta agggggtttc ataatgacta agagtaatta
    25261 ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag
    25321 cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc
    25381 gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa
    25441 aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct
    25501 ttttttcttc attccactga atactaggat gctggttcat gatcccgata agcgtatctg
    25561 tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg
    25621 ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct
    25681 atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt
    25741 tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac
    25801 aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat
    25861 tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt
    25921 ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt gaacgtctgc
    25981 ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag
    26041 gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca
    26101 tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct
    26161 aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca
    26221 gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctctt
    26281 cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg
    26341 ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc
    26401 gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag
    26461 atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca
    26521 attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat
    26581 gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat
    26641 ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac catggataat
    26701 tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg
    26761 ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca
    26821 tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc
    26881 acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc
    26941 tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc
    27001 agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc
    27061 aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga
    27121 ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc
    27181 accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt
    27241 tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg
    27301 tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc
    27361 agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct
    27421 tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg
    27481 attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct
    27541 cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa
    27601 acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg
    27661 gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat
    27721 tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc taatactcag
    27781 gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat gaatcccaca
    27841 aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt agctgctaac
    27901 atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact ataagtcaaa
    27961 ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt
    28021 tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc agttacttgt
    28081 gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa aatccctgta
    28141 gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga ttctacaaat
    28201 gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga atcagaagct
    28261 aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg gtttgctaat
    28321 ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag cactacgacc
    28381 cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa tgttggtttc
    28441 aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt gaccagacct
    28501 aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg aacaagcaag
    28561 atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct
    28621 aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag accaaggaaa
    28681 gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg gatcgcatca
    28741 atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga agcaacgaat
    28801 acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga ataagcaaaa
    28861 aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa tacccccatt
    28921 aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc ttcatccatt
    28981 ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga gataaatgtt
    29041 gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat ttcgaagtag
    29101 gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa ataaagtccg
    29161 ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa caaaacataa
    29221 gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt
    29281 atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa
    29341 gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg
    29401 ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc
    29461 tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc
    29521 tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat
    29581 tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa
    29641 aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta tctgtgattt
    29701 ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag
    29761 aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc
    29821 ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga
    29881 ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt
    29941 cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc tttctatttg
    30001 attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt
    30061 ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt
    30121 tttttacaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc ttttataatt
    30181 ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat
    30241 cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga
    30301 tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt
    30361 cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg
    30421 gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt
    30481 ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa
    30541 aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt
    30601 cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga
    30661 atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg
    30721 taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac
    30781 tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag
    30841 gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca cacctcttga
    30901 ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac
    30961 atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg
    31021 ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt
    31081 atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc
    31141 gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag
    31201 gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag
    31261 ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta aatgctccgg
    31321 tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc
    31381 tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata
    31441 gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt
    31501 atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc
    31561 attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg
    31621 atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga
    31681 ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag
    31741 tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat actgtcctct
    31801 tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc gggcaatcat
    31861 tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag
    31921 ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa
    31981 taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg
    32041 tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc
    32101 aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc
    32161 tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata
    32221 tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct
    32281 gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat
    32341 ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct
    32401 tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg
    32461 agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc
    32521 gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct
    32581 tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct
    32641 tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat tacggcaaat
    32701 aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat
    32761 tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc
    32821 cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca
    32881 ggacagcttt ttcacaacct ccccactaaa cggtgttctc ctaccaactc ctcggtcaca
    32941 agtcacaata ttcatcaaac atgagaagct gtttttttga aattgctcct tgtgactcgg
    33001 agctgaacga caggctcacg acctctcggt aaacagcgtt caaaagccaa tttttcgata
    33061 ttaagccatt tctttgttac ttattgtcat tgatttcttt ttctaattta tccaatagtg
    33121 gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga
    33181 atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg
    33241 tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca
    33301 aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg
    33361 ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg
    33421 ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat
    33481 acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt
    33541 cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga
    33601 tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac
    33661 caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt
    33721 ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca
    33781 tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct
    33841 ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg
    33901 ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg
    33961 gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat
    34021 ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct
    34081 acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt
    34141 ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg
    34201 gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca
    34261 tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg
    34321 gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca
    34381 ttgactaaca catgcatgcc gatatattta gcggtatcga cacttgtttg aataatgatc
    34441 ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa gcatttctag gttatgcgta
    34501 ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt
    34561 gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt
    34621 tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc
    34681 agcacgagca gcatctgcac gcttctggtt atagaggctc atcagttttt tatacccatc
    34741 tccgatcgtc tgtttatttt ccaaactctt ttctaaatat tggatggctt gatcgttcac
    34801 atacaattca gaaaatttaa gtccaacttg ttcataaagt ttcgctaatt tatgcttatc
    34861 ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc
    34921 ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt tttctttttt
    34981 cttaaaaaat ccaaacatca ttttccctac tttcaattaa aatgctttca aaactgggct
    35041 taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg ctgtagcatt
    35101 gttgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg taataaagaa
    35161 accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat
    35221 ccctgctgtc cctccggcaa agaaattggt tagcatacca ggtaaaatca tcgctaagcc
    35281 gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa
    35341 aatgacagca tttgggctat aaggaaagaa aactggacaa tctaaggcag ggatcgcatc
    35401 aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta acagtagacg
    35461 tacaccagct agcaatacat acacacccac tacgaattgg atggcttgta aaaaggcaaa
    35521 catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg caaataaaac
    35581 agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat cttgcagaaa
    35641 ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc ctttctcacc
    35701 aaaaagctta gcaacaccgg cttcaaataa gtacccgatc gtacaaaaat gacctaaagc
    35761 aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg gcatagcaat
    35821 ggcgaaacaa gctccgataa aaccaccgac caagatcaat acgatccctc gcagaccggc
    35881 aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac cagttaaaaa
    35941 aatgtacttc cattttgtga aacgagccaa aataatattg aatacaaaaa tggctaagaa
    36001 cgtcagtgca atatctctac ctaaacccaa atcattcatt gcttgaccat tgatagattc
    36061 aatagaaggg atgatccctt gcatatgaaa tccttcagta aagattttcc cgaaataagt
    36121 taaacttcca acgatgatgc tcgaaccggc actcaacact tgaaatccta ggagtgtctt
    36181 taacgtgcca gagatgactt gtcctgctga tttcttctgc agtagtagac ccaacatagc
    36241 aatcaatgca atggtgatcg atgcctgagt caaaatattt tcgatgataa aattgataac
    36301 gcccatg
//
LOCUS       DASJTM010000021        35927 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJTM010000021 DASJTM010000000
VERSION     DASJTM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.021"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            238..1379
                     /locus_tag="U4B29_000953"
                     /pseudo
     CDS             238..1379
                     /locus_tag="U4B29_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1505..2584)
                     /locus_tag="U4B29_000954"
     CDS             complement(1505..2584)
                     /locus_tag="U4B29_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2105918.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2693..3133)
                     /locus_tag="U4B29_000955"
     CDS             complement(2693..3133)
                     /locus_tag="U4B29_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2105919.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3190..3600)
                     /locus_tag="U4B29_000956"
     CDS             complement(3190..3600)
                     /locus_tag="U4B29_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2105920.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3781..4215)
                     /locus_tag="U4B29_000957"
     CDS             complement(3781..4215)
                     /locus_tag="U4B29_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2105921.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4315..5163)
                     /locus_tag="U4B29_000958"
     CDS             complement(4315..5163)
                     /locus_tag="U4B29_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2105922.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5552..7318
                     /locus_tag="U4B29_000959"
     CDS             5552..7318
                     /locus_tag="U4B29_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN2105923.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7420..8679
                     /locus_tag="U4B29_000960"
     CDS             7420..8679
                     /locus_tag="U4B29_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2105924.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8826..10127
                     /locus_tag="U4B29_000961"
     CDS             8826..10127
                     /locus_tag="U4B29_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2105925.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10130..10981
                     /locus_tag="U4B29_000962"
     CDS             10130..10981
                     /locus_tag="U4B29_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2105926.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10994..11809
                     /locus_tag="U4B29_000963"
     CDS             10994..11809
                     /locus_tag="U4B29_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN2105927.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11960..13126
                     /locus_tag="U4B29_000964"
     CDS             11960..13126
                     /locus_tag="U4B29_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2105928.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13356..14003
                     /locus_tag="U4B29_000965"
     CDS             13356..14003
                     /locus_tag="U4B29_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2105929.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14087..15031
                     /locus_tag="U4B29_000966"
     CDS             14087..15031
                     /locus_tag="U4B29_000966"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN2105930.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGV
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15166..16617
                     /locus_tag="U4B29_000967"
     CDS             15166..16617
                     /locus_tag="U4B29_000967"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN2105931.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16638..17474
                     /locus_tag="U4B29_000968"
     CDS             16638..17474
                     /locus_tag="U4B29_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2105932.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17474..18133
                     /locus_tag="U4B29_000969"
     CDS             17474..18133
                     /locus_tag="U4B29_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2105933.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18259..18948
                     /locus_tag="U4B29_000970"
     CDS             18259..18948
                     /locus_tag="U4B29_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105934.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18954..19910
                     /locus_tag="U4B29_000971"
     CDS             18954..19910
                     /locus_tag="U4B29_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN2105935.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19954..20466
                     /locus_tag="U4B29_000972"
     CDS             19954..20466
                     /locus_tag="U4B29_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2105936.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20722..21270)
                     /locus_tag="U4B29_000973"
     CDS             complement(20722..21270)
                     /locus_tag="U4B29_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2105937.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21359..22540)
                     /locus_tag="U4B29_000974"
     CDS             complement(21359..22540)
                     /locus_tag="U4B29_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN2105938.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22776..24284)
                     /locus_tag="U4B29_000975"
     CDS             complement(22776..24284)
                     /locus_tag="U4B29_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN2105939.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24443..24634
                     /locus_tag="U4B29_000976"
     CDS             24443..24634
                     /locus_tag="U4B29_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105940.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24837..25754
                     /locus_tag="U4B29_000977"
     CDS             24837..25754
                     /locus_tag="U4B29_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN2105941.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25747..26820
                     /locus_tag="U4B29_000978"
     CDS             25747..26820
                     /locus_tag="U4B29_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105942.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26968..27486
                     /locus_tag="U4B29_000979"
     CDS             26968..27486
                     /locus_tag="U4B29_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105943.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27467..27859
                     /locus_tag="U4B29_000980"
     CDS             27467..27859
                     /locus_tag="U4B29_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN2105944.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27935..28870
                     /locus_tag="U4B29_000981"
     CDS             27935..28870
                     /locus_tag="U4B29_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN2105945.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29003..29782
                     /locus_tag="U4B29_000982"
     CDS             29003..29782
                     /locus_tag="U4B29_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2105946.1"
                     /translation="MNKKKSAPWLQALHAVMPLYISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29775..30092
                     /locus_tag="U4B29_000983"
     CDS             29775..30092
                     /locus_tag="U4B29_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2105947.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30336..31946
                     /locus_tag="U4B29_000984"
     CDS             30336..31946
                     /locus_tag="U4B29_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2105948.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32103..32951
                     /locus_tag="U4B29_000985"
     CDS             32103..32951
                     /locus_tag="U4B29_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2105949.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33182..33661
                     /locus_tag="U4B29_000986"
     CDS             33182..33661
                     /locus_tag="U4B29_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105950.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33694..35922
                     /locus_tag="U4B29_000987"
     CDS             33694..35922
                     /locus_tag="U4B29_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2105951.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT        11980 a         6423 c         7043 g        10481 t
ORIGIN      
        1 acgatgttca gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagat
       61 aaacgtttta atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta
      121 aaacttgaaa atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag
      181 gtagaatctc tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg
      241 agatattcaa ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat
      301 cagacagtcc acacagagga gacagaaaca gccaaatgga cagaaaccaa gtgggccaac
      361 gtcaatcgaa ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta
      421 agaaaaggac aaacagcgac catcaaagca aaaattgact attcgggaga cttagcacct
      481 atactacaat cagctgttac atttgagaat gttgatccca ctttgtcgat tggctatgac
      541 gagaacactt tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat
      601 caggacttgt taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat
      661 catcatttaa cgccgtatag tcagcggcag acaattgagc aagattcagc catcgactca
      721 ggaggagatc ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta
      781 ccgcctacag aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc
      841 gatacagaaa ctcaaccaga gccagacaat aaatcaaaac ctgaaataac ccctggagaa
      901 aaaccagatg taactccaga accagatacg gattctggaa accaaacagt accggaaaca
      961 aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtaga tccagaagaa
     1021 aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa
     1081 ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca
     1141 attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa
     1201 tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa acgaaaaata
     1261 aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc
     1321 tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac
     1381 atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaatgaccc
     1441 caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt
     1501 tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata
     1561 catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc
     1621 gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca
     1681 tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga
     1741 tataaacaac atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag
     1801 aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca
     1861 gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag
     1921 cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat
     1981 agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa
     2041 ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca
     2101 ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct
     2161 gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat
     2221 cttttggctg atgctcgtaa aaatcaataa cggactggat catatcaaat gtttgatgaa
     2281 taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa
     2341 tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat
     2401 gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta
     2461 agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca
     2521 aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat
     2581 tcattatatg atggaatatg aaaataagaa agagaaagag caaaaagaaa aaccgctaaa
     2641 tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct
     2701 ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca
     2761 attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt
     2821 tttgcaacgc agcaccttga ggagtcagcg taacatttac gattctttca tcacccgttt
     2881 cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac
     2941 cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata
     3001 aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg agggaagtgt
     3061 agtgtctaat gatttcctta gaggcggcat ataaaggaaa acatacttga ttaactaaat
     3121 atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca
     3181 caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt
     3241 tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag
     3301 accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata
     3361 aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa
     3421 ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt
     3481 tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt
     3541 atgaacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat
     3601 aaaattacca cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa
     3661 agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt
     3721 ctcgaatagc aaacatatac aaaaaaatat cagagacaaa aactaatctc atcataaaca
     3781 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa cttacttcac gatggacctg
     3841 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
     3901 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
     3961 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
     4021 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtgc catacaaggt
     4081 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
     4141 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatctatttc
     4201 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
     4261 attataccag ttaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
     4321 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
     4381 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
     4441 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
     4501 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
     4561 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
     4621 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa caaacagatt
     4681 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
     4741 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
     4801 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
     4861 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaacaagc ttccgaaaag
     4921 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
     4981 caacactaaa ataactatag ctcggtcaaa tcctaaaaca gcttccagta atattcctag
     5041 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
     5101 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
     5161 catttatcca cttccttaat tcattaataa tactcttatc ttattataac ttacttttca
     5221 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
     5281 ttctagaata tacttctacg ttaaaaactg ttaaaaaact tttactttag aaaattggag
     5341 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag attaaaactt
     5401 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
     5461 actgttaccg ataacatttt tgaaagcgtt gtcttttgtt tctctatcac ttatagtgat
     5521 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
     5581 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
     5641 gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat ccatatacca taacaactga
     5701 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
     5761 ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag tatgcgttcc atgttgttgg
     5821 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
     5881 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
     5941 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
     6001 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
     6061 agaagacttc ttcgggggag atttgcaagg tgtgagggat catttggatt acttgacgga
     6121 ccttggcgtc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
     6181 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
     6241 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
     6301 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
     6361 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
     6421 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
     6481 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
     6541 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
     6601 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
     6661 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
     6721 attcacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
     6781 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
     6841 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
     6901 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
     6961 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
     7021 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
     7081 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtat atgatcgtga
     7141 agataagctc tatttcacac gggaactaga gggacagatc atctatgttt actttaatca
     7201 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
     7261 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
     7321 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
     7381 gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
     7441 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
     7501 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
     7561 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
     7621 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
     7681 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
     7741 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
     7801 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
     7861 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
     7921 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
     7981 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
     8041 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
     8101 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
     8161 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
     8221 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
     8281 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
     8341 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
     8401 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
     8461 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
     8521 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
     8581 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
     8641 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
     8701 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
     8761 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
     8821 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
     8881 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
     8941 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
     9001 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
     9061 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
     9121 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
     9181 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
     9241 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
     9301 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
     9361 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
     9421 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
     9481 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
     9541 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
     9601 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
     9661 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
     9721 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
     9781 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
     9841 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
     9901 tcaataattt ctccatgatc tatctgttta acaatggtgg tcccggaagc gtaggcggag
     9961 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
    10021 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
    10081 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
    10141 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
    10201 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
    10261 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
    10321 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
    10381 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
    10441 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
    10501 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
    10561 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
    10621 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
    10681 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
    10741 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
    10801 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
    10861 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
    10921 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
    10981 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
    11041 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
    11101 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
    11161 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
    11221 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
    11281 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
    11341 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
    11401 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
    11461 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
    11521 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
    11581 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
    11641 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
    11701 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
    11761 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
    11821 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
    11881 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
    11941 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
    12001 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
    12061 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
    12121 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
    12181 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
    12241 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
    12301 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
    12361 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
    12421 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
    12481 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
    12541 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
    12601 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
    12661 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
    12721 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
    12781 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
    12841 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
    12901 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
    12961 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
    13021 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
    13081 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
    13141 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
    13201 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
    13261 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
    13321 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
    13381 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
    13441 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
    13501 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
    13561 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
    13621 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
    13681 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
    13741 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
    13801 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
    13861 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
    13921 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
    13981 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
    14041 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
    14101 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
    14161 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
    14221 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
    14281 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
    14341 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
    14401 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
    14461 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
    14521 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
    14581 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
    14641 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
    14701 agaacaagaa gcagtagtca tcaaaccaaa agaaggggtc ggcggcattg gcggagagta
    14761 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
    14821 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
    14881 tggcgcaagc atgttacaga ttggtacaga gcttcataaa gaagggccgg aaattttttc
    14941 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
    15001 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
    15061 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
    15121 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
    15181 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    15241 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    15301 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    15361 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    15421 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    15481 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    15541 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    15601 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    15661 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    15721 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    15781 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    15841 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    15901 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    15961 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    16021 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    16081 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    16141 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    16201 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    16261 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    16321 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    16381 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    16441 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    16501 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    16561 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    16621 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    16681 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    16741 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    16801 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    16861 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    16921 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    16981 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    17041 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    17101 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    17161 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    17221 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    17281 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    17341 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    17401 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    17461 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    17521 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    17581 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    17641 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    17701 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    17761 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    17821 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    17881 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    17941 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    18001 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    18061 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    18121 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    18181 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    18241 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    18301 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    18361 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    18421 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    18481 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    18541 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    18601 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    18661 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    18721 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    18781 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    18841 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    18901 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    18961 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    19021 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    19081 aaaatgtgat cgaattattt caaaaaaagc accatcgaat cgctgtgatc caaaatcccc
    19141 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    19201 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    19261 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    19321 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    19381 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    19441 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    19501 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    19561 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    19621 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    19681 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    19741 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    19801 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    19861 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    19921 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    19981 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    20041 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    20101 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    20161 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    20221 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    20281 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    20341 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    20401 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    20461 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    20521 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    20581 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    20641 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    20701 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    20761 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    20821 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    20881 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    20941 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    21001 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    21061 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    21121 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    21181 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    21241 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    21301 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    21361 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    21421 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    21481 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    21541 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    21601 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    21661 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    21721 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    21781 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    21841 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    21901 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    21961 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    22021 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    22081 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    22141 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    22201 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    22261 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    22321 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    22381 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    22441 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    22501 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    22561 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    22621 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    22681 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    22741 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    22801 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    22861 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    22921 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    22981 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    23041 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    23101 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    23161 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    23221 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    23281 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    23341 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    23401 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    23461 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    23521 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    23581 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    23641 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    23701 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    23761 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    23821 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    23881 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    23941 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    24001 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    24061 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    24121 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    24181 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    24241 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    24301 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    24361 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    24421 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    24481 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    24541 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    24601 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    24661 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    24721 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    24781 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    24841 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    24901 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    24961 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    25021 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    25081 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    25141 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    25201 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    25261 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    25321 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    25381 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    25441 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    25501 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    25561 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    25621 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    25681 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    25741 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    25801 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    25861 gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac tccgacgaat
    25921 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    25981 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    26041 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    26101 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    26161 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    26221 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    26281 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    26341 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    26401 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    26461 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    26521 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    26581 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    26641 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    26701 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    26761 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    26821 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    26881 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    26941 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    27001 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    27061 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    27121 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    27181 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    27241 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    27301 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    27361 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    27421 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    27481 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    27541 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    27601 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa aaggcgatga
    27661 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    27721 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    27781 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    27841 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    27901 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    27961 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    28021 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    28081 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    28141 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    28201 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    28261 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    28321 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    28381 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    28441 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    28501 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    28561 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    28621 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    28681 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    28741 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    28801 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    28861 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    28921 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    28981 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    29041 gcatgcagtg atgccacttt atataagcta tataccagta ggattagctt gcggagtatt
    29101 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    29161 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    29221 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    29281 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    29341 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    29401 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    29461 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    29521 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    29581 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    29641 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    29701 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    29761 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    29821 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    29881 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    29941 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    30001 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    30061 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    30121 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    30181 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    30241 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    30301 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    30361 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    30421 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    30481 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    30541 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    30601 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    30661 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    30721 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    30781 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    30841 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    30901 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    30961 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    31021 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    31081 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    31141 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    31201 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    31261 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    31321 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    31381 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    31441 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    31501 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    31561 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    31621 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    31681 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    31741 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    31801 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    31861 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    31921 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    31981 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    32041 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    32101 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    32161 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    32221 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    32281 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    32341 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    32401 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    32461 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    32521 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac gcttatctga
    32581 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    32641 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    32701 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    32761 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    32821 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    32881 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    32941 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca tcatcttgga
    33001 ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta tttctatcaa
    33061 tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca tatagtttag
    33121 taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag gagttaaaat
    33181 aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag actatgataa
    33241 agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg gccttgctca
    33301 gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata ctggagaaaa
    33361 atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag cgatcttagc
    33421 tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt atgatccaga
    33481 aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag aagtagatac
    33541 caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac aaaaagacgg
    33601 agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc taggatatta
    33661 atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact aacagaaaac
    33721 cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag ccatcggaca
    33781 agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga tcctgaaatg
    33841 aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca attagccaaa
    33901 gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc agatatggac
    33961 aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga acaagaccaa
    34021 gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac actgatccag
    34081 aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct agttcaaagg
    34141 atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga aatcgttcga
    34201 gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg agatgcctct
    34261 gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt accgacagga
    34321 atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa ttaccaagga
    34381 aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa aatggatctt
    34441 cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca aaaacaagaa
    34501 cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa agctaacgcc
    34561 atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta taagcaagga
    34621 caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac tgattcagat
    34681 gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga acttcaggac
    34741 atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg aagaaccatt
    34801 agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat tctgcgctta
    34861 gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa acaagaagaa
    34921 acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag cgatatcgaa
    34981 ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc tgcttctgac
    35041 atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg acaaatcatg
    35101 aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt ccaaggaaaa
    35161 acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc acttagccgg
    35221 caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg aaaatacgaa
    35281 ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt tgacaaaaag
    35341 aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga atctgggtca
    35401 atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat tgccgaattt
    35461 tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg ttcgtcaaga
    35521 gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg gttaaatgaa
    35581 aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact tcgtttagca
    35641 gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat tagtgacggt
    35701 cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat ccagtctgtt
    35761 ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg ccaagacaaa
    35821 gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga cttgaatgaa
    35881 tttcccaagc aaatgattca actgattgcc cgctatctat gataaaa
//
LOCUS       DASJTM010000022        35235 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJTM010000022 DASJTM010000000
VERSION     DASJTM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.022"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(149..634)
                     /locus_tag="U4B29_000988"
     CDS             complement(149..634)
                     /locus_tag="U4B29_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN2105952.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    724..1069
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1114..1755
                     /locus_tag="U4B29_000989"
     CDS             1114..1755
                     /locus_tag="U4B29_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2105953.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1774..2409
                     /locus_tag="U4B29_000990"
     CDS             1774..2409
                     /locus_tag="U4B29_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2105954.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2427..3242
                     /locus_tag="U4B29_000991"
     CDS             2427..3242
                     /locus_tag="U4B29_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2105955.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3561..4997
                     /locus_tag="U4B29_000992"
     CDS             3561..4997
                     /locus_tag="U4B29_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2105956.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5186..7633
                     /locus_tag="U4B29_000993"
     CDS             5186..7633
                     /locus_tag="U4B29_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2105957.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7863..8930
                     /gene="rlmN"
                     /locus_tag="U4B29_000994"
     CDS             7863..8930
                     /gene="rlmN"
                     /locus_tag="U4B29_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2105958.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9123..10226)
                     /locus_tag="U4B29_000995"
     CDS             complement(9123..10226)
                     /locus_tag="U4B29_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2105959.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10779..11327
                     /gene="aac(6')"
                     /locus_tag="U4B29_000996"
     CDS             10779..11327
                     /gene="aac(6')"
                     /locus_tag="U4B29_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2105960.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11873..13534
                     /locus_tag="U4B29_000997"
     CDS             11873..13534
                     /locus_tag="U4B29_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2105961.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13604..13951)
                     /locus_tag="U4B29_000998"
     CDS             complement(13604..13951)
                     /locus_tag="U4B29_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN2105962.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14035..14976
                     /locus_tag="U4B29_000999"
     CDS             14035..14976
                     /locus_tag="U4B29_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105963.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            14977..16020
                     /locus_tag="U4B29_001000"
     CDS             14977..16020
                     /locus_tag="U4B29_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2105964.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16026..17096
                     /locus_tag="U4B29_001001"
     CDS             16026..17096
                     /locus_tag="U4B29_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105965.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17086..18039
                     /locus_tag="U4B29_001002"
     CDS             17086..18039
                     /locus_tag="U4B29_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2105966.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18051..19061
                     /locus_tag="U4B29_001003"
     CDS             18051..19061
                     /locus_tag="U4B29_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2105967.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19346..20167)
                     /locus_tag="U4B29_001004"
     CDS             complement(19346..20167)
                     /locus_tag="U4B29_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2105968.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20264..20542)
                     /locus_tag="U4B29_001005"
     CDS             complement(20264..20542)
                     /locus_tag="U4B29_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105969.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20810..22819
                     /gene="metG"
                     /locus_tag="U4B29_001006"
     CDS             20810..22819
                     /gene="metG"
                     /locus_tag="U4B29_001006"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN2105970.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23298..23885)
                     /locus_tag="U4B29_001007"
     CDS             complement(23298..23885)
                     /locus_tag="U4B29_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105971.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24060..25382
                     /locus_tag="U4B29_001008"
     CDS             24060..25382
                     /locus_tag="U4B29_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2105972.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25537..26313
                     /locus_tag="U4B29_001009"
     CDS             25537..26313
                     /locus_tag="U4B29_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2105973.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26306..26875
                     /gene="rnmV"
                     /locus_tag="U4B29_001010"
     CDS             26306..26875
                     /gene="rnmV"
                     /locus_tag="U4B29_001010"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN2105974.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26868..27752
                     /gene="rsmA"
                     /locus_tag="U4B29_001011"
     CDS             26868..27752
                     /gene="rsmA"
                     /locus_tag="U4B29_001011"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN2105975.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28203..28832
                     /locus_tag="U4B29_001012"
     CDS             28203..28832
                     /locus_tag="U4B29_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105976.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(28956..30065)
                     /gene="ugpC"
                     /locus_tag="U4B29_001013"
     CDS             complement(28956..30065)
                     /gene="ugpC"
                     /locus_tag="U4B29_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN2105977.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30274..30696
                     /gene="mgsA"
                     /locus_tag="U4B29_001014"
     CDS             30274..30696
                     /gene="mgsA"
                     /locus_tag="U4B29_001014"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN2105978.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31019..31555
                     /locus_tag="U4B29_001015"
     CDS             31019..31555
                     /locus_tag="U4B29_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2105979.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31595..33358)
                     /locus_tag="U4B29_001016"
     CDS             complement(31595..33358)
                     /locus_tag="U4B29_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105980.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33361..35076)
                     /locus_tag="U4B29_001017"
     CDS             complement(33361..35076)
                     /locus_tag="U4B29_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2105981.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11468 a         6277 c         7299 g        10191 t
ORIGIN      
        1 tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt tgttacattg
       61 tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa agacgcttga
      121 attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc agatgttcta
      181 agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg gtattgatat
      241 ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg acttggattt
      301 ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga acgcctagga
      361 ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat tgttcagcta
      421 gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt tgttttcctt
      481 ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa gggacatttt
      541 tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt ttttttagta
      601 gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct ttttttatta
      661 gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca cgtaaagtta
      721 aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt ctctggttgg
      781 tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct gaagcgtata
      841 gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc agataaggct
      901 ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc
      961 ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga ggttttttct
     1021 gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg catttgccaa
     1081 gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga agttatgccg
     1141 caattattag taggagcact cacaacattg aaattatttt tatttacctt gattggctca
     1201 ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc gctacgctac
     1261 ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca actgattttt
     1321 attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc agcactcatt
     1381 gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg gatacaatcg
     1441 attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt tcaaacggtg
     1501 acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg gaatgaagtt
     1561 atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga cgtaatgcgt
     1621 gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg cgtggcaatc
     1681 atctatctta cattgactgg tattctaaca atcataatga aacaagttga aggaaaatta
     1741 aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa agtgaacaaa
     1801 gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga tgggaaaaca
     1861 ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat cttggctggc
     1921 ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa cccattcgag
     1981 cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct atttccacat
     2041 ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca gacaaaagaa
     2101 gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga tttattgaat
     2161 atctatcctt atcagctttc tggcggacaa aaacagcggg tagctattgc cagagcattg
     2221 gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga tcctgaatta
     2281 cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac ccaaattatt
     2341 gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa agtttcccca
     2401 attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc gctttgatcg
     2461 caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat aattgggcga
     2521 ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt cctatgggat
     2581 ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa gcagtctttg
     2641 aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag gaatttgaac
     2701 tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg gcaagagaaa
     2761 agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc acacctaaag
     2821 aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg caaaatgggt
     2881 catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata gtcaaaaacc
     2941 aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa agcagacggg
     3001 tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa agaaacgaac
     3061 tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca gttggtgcac
     3121 gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa
     3181 caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg ccgagaaatt
     3241 aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa taaacagtgt
     3301 tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct cttggtaaac
     3361 ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     3421 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcctttaca
     3481 taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac gattgaaggt
     3541 tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca aaagattttc
     3601 tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag
     3661 gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa ggaacgaacg
     3721 gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta atgaaagaac
     3781 aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct aatggacgag
     3841 gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa
     3901 acgatattga acctatgttg actttatatc attgggatct tccacaagca ttgcaagatg
     3961 aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat gcagaaacat
     4021 tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa caaaatatct
     4081 tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac ccgaaacgaa
     4141 tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat aaatttcacg
     4201 agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat tatccaattg
     4261 acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg gccaattatt
     4321 ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc ttagaagaaa
     4381 aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct gctaaaccag
     4441 attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca ttaaacggag
     4501 ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa actggagttc
     4561 ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg gactgggaga
     4621 ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac cgcatgccgg
     4681 tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat aaacaaatcc
     4741 atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa gaagcaattt
     4801 cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg cttagctggc
     4861 tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa acacaagaag
     4921 gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg attgaagaaa
     4981 atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga tatggccgtg
     5041 ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc tgaaaaatga
     5101 aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt tgtataatga
     5161 aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta aaaaaaggaa
     5221 aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga cgattggtgt
     5281 tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt ttttaggaat
     5341 attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc tctgtctttt
     5401 attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa ttggtaaaaa
     5461 ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt tacatgccca
     5521 tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat gggatttgtt
     5581 agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga tgattggagc
     5641 aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt tagcagcagt
     5701 gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc agctgttaaa
     5761 tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc ctgccaagca
     5821 agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag cgaaagaagc
     5881 aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg agaagaaaaa
     5941 agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga
     6001 gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa
     6061 tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga
     6121 tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa
     6181 tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag
     6241 tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt
     6301 cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt
     6361 ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct
     6421 tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg
     6481 tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca
     6541 accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt
     6601 acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg
     6661 gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga
     6721 agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc
     6781 tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt
     6841 agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc
     6901 tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt
     6961 accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga
     7021 agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat
     7081 cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc
     7141 ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa
     7201 tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa
     7261 accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt
     7321 tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga
     7381 aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt
     7441 cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa
     7501 ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg
     7561 aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga
     7621 taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca
     7681 aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat
     7741 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
     7801 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
     7861 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
     7921 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
     7981 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
     8041 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
     8101 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
     8161 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
     8221 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
     8281 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
     8341 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
     8401 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
     8461 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
     8521 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
     8581 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
     8641 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     8701 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     8761 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     8821 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     8881 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     8941 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     9001 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     9061 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     9121 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     9181 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     9241 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     9301 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     9361 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     9421 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     9481 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     9541 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     9601 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     9661 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     9721 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     9781 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     9841 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     9901 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     9961 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
    10021 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
    10081 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
    10141 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
    10201 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
    10261 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
    10321 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
    10381 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
    10441 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
    10501 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
    10561 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
    10621 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
    10681 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
    10741 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
    10801 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
    10861 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
    10921 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
    10981 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
    11041 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
    11101 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
    11161 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
    11221 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
    11281 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
    11341 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
    11401 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
    11461 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    11521 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttattcagat
    11581 aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt ttatctttgt
    11641 atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga ccatggaaaa
    11701 ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa aatcattatt
    11761 ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa actcttaaac
    11821 aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa
    11881 gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat gttatggcgg
    11941 cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag gaggcgtatt
    12001 taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa cagatacaat
    12061 cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg aagacagcaa
    12121 accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat taacttacaa
    12181 aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag cagcggatta
    12241 cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg cttacctgta
    12301 tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat cagaactagg
    12361 gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa caccatattt
    12421 ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag tggaagaaaa
    12481 tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat tcacattaga
    12541 agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg atcattactg
    12601 ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag aatcttctac
    12661 tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg gtgagttagc
    12721 acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga caagctatat
    12781 cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa gaaaagctat
    12841 ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg gatcagttgt
    12901 ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg aagactttgc
    12961 aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat attgggaaaa
    13021 agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt ctgatgatga
    13081 ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact tggatggcgt
    13141 aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa cttcgggtga
    13201 ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca gctttactga
    13261 tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag aatatgacga
    13321 agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt gggatgattt
    13381 ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt accaaaaagc
    13441 agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc
    13501 atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag catgagaaaa
    13561 aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct aacaaagaaa
    13621 aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc caaaagctgt
    13681 acataccgta gctggcagac gagagcgagg aaaattctgg ttttttcttt cggttccttg
    13741 ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa aagacccata
    13801 aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta gataattgaa
    13861 tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc agtagaaaag
    13921 ctactgcgta ggggatagat ttttttttca tttgattgct cctttatttt tggttcatta
    13981 tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac attagtgaat
    14041 agttatatca aatatgtatt aaaacgtgtc tttttcatga tcatcacgct ttggttaatt
    14101 gcaacaatca cctttttctt gatgcaattg ctgccgggca cgccatatac gaatcaggaa
    14161 cgtttgagtc ctgaaacaat cgagatgcta aataaacaag tcggtttgga taaaccagtt
    14221 atcgtacaat atgggattta tctttctaat ttgcttcaag gagattttgg aatttccttc
    14281 caatttaaaa accagccagt cgcacatttg ttagctggaa gaatcggacc atccttgcag
    14341 ttagggttac aggcaattat ctttggaaca gtcctaggga cgattttagg aacgatctct
    14401 gccatgaagc aaaatacatg ggcagataca tcttctacac tcgtagcgat attaggtcgt
    14461 tccattccaa actttgtatt tgctgtttta ttacaatata tctttgcaat caaattacaa
    14521 gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac aattgcgttg
    14581 gccatgtcgc cgcttgctga ctctgctcgg tttatccgta cggaaatggt ggaagtactg
    14641 catagtgatt atgtggaatt agcaaaagca aaaggattga gccgttggga aatcgccttt
    14701 aaacatggtc ttcgaaatag tttgattcca ttgatgactt tattaggacc gttagctgta
    14761 gcattgatga ccggatcact ggtggtagaa aatatctttg ctattcctgg tatcggggaa
    14821 caattcgtta agtcaatcac aacaaatgat tatcctacga tcatggctgt aacgatcctt
    14881 tactcattta tgctgatttt tgttattttg atcgtggatc tattgtacgg tctagttgat
    14941 ccaagaattc gtgtttcaga agggagccga ggataaatgg aaatgatacc aaagcgttat
    15001 gaaacagttg cagatatccc tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa
    15061 gaacgtgaac agattactgc accttctttg aattttatcc aagattcatg gcgccgttta
    15121 aagaaaaaca aagctgccgt tatttcaatg gttttactat tggtcatcat tttgatctct
    15181 gtgatcacaa tctttgtttc tcctcatgat ccaactgccc aaaacgtggc ttatatcaat
    15241 ttgcctcctc gtattccggg aatcaatatc gatggattga atggaaaagc aatggttggt
    15301 ggtgaacttg ttgataaata tgctcaagca aacgtccctt caaacgtaaa tttcttttta
    15361 ggaacagacg gtttgggacg tgatgtatta agccgcttat ttatgggaat gagaatctcg
    15421 ttgctgatcg catttattgc tgcattacta gatgtaacga ttggtgtagc ttacgggttg
    15481 gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta
    15541 tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga accaggaatt
    15601 ttttcaatcg tggctgctat ggccatcaca aactggatac caatggcacg tatcgttcgt
    15661 gcacaaacaa tgaaattaaa agatcaagaa tatgttttag ctggattgac attaggtgaa
    15721 tcaagaatga aaattgcatt caaacatatt ttgccgaata tctctagtgt cattattatt
    15781 caaatgatgt ttagtattcc atctgctatt ttcttcgaag cctttttaag ctttatcggt
    15841 ttaggtctta cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg gtacaagaca
    15901 ttcctgtatc tgccttatct attatggatt ccggcagtaa ctttatcggt tatcatgatt
    15961 ggctttaact tacttgctga tggattgcgt gatgcctttg atcctaaaat gaaagagtga
    16021 agacgatgac gaaaatttta gaagtgaaag acttagaaat ctcttttgat acgtatgctg
    16081 gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa acattggcga
    16141 tcgttgggga atctggaagt ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt
    16201 caagcaacgc caatatagat gctggtcaaa tcctctttaa aggtaacgat atcgttggaa
    16261 aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc
    16321 caatgacctc tttagatccg acgatgccca ttgggaagca agtcgctgag tcccttagaa
    16381 agcataataa agtttcaaaa aaagagggac aaaaagctgc attagaattg cttaaattag
    16441 ttggaatacc cgaagctgag aaacgtatca acagttatcc gcatcagttc tcaggaggtc
    16501 agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta tccggaaata ctgattgctg
    16561 atgaacctac aacagcgctt gatgtgacta ttcaagcaca gattttggag ctgctgaaag
    16621 aaattcagac aaaaatcgat acatcaatta tctttatcac tcatgacctt ggtgtagtag
    16681 cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa gttggaacat
    16741 cggaagaaat tttctacaac ccgcaacatc cctatacttg gggactgctt ggttcaatgc
    16801 cgacattaga tagtgcgaac gatcgattgt atgctattcc tggttctcca ccggatttat
    16861 tgaacccgcc aaaaggagac gcattctatc caagaaatga attcgcgatg aaaatcgatg
    16921 cagaacaaga accaccattt tttgaactat caaaaacaca caaggcagca acttggctac
    16981 ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct
    17041 ttaaagagaa acaaaaacat acttacggtc aaggaggaat tgctgatgcc aaataaacta
    17101 cttgaagtta aaggattgaa acagtatttc aatgtaggaa aaaagaatga agtacatgct
    17161 gtaaatgaca tcagctttca catttatgaa ggagaaacat tcggtttagt tggagaatct
    17221 ggaagcggaa aatccactac tggccgtacg atcattcgct tgaatgaacc tactgatggt
    17281 gagatattat tcgacggtca agatataacg aagatcaaag ataaaaaagg tttgacgaaa
    17341 ttccggcatg atgttcagat gatctttcaa gatccatacg cttcgttgaa tcctcgtatg
    17401 aaagtcagag atatcattgc cgaaggaatt gatgttaatg gactagctaa atcatctgaa
    17461 gagcgtgcaa aaaaagtcga tgatctattg agaactgttg gattgaatcc aagccacggt
    17521 actcgttatc ctcatgaatt ttcaggtggg caacgccagc gtattggtat tgcacgagca
    17581 ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa tttctgcact agatgtgtcg
    17641 atccaagccc aagtggtcaa tttgttgcaa gatctccaaa aagaacatca attgacctat
    17701 ctatttattg cacatgattt gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg
    17761 cataacggct tgttattaga gatgggaaca agtgatgaaa tctataatca cggtgttcat
    17821 ccttatacag aaagtttgtt atctgctatt cctctaccag atccagacca tgagagaaaa
    17881 cgcagaagaa tcaaatatca acctgaacca gatgacggac aagtacgtca acttcgtgaa
    17941 atcgcaccag aacattttgt ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa
    18001 ctaaaacgac aaaaggaatc tcttctggtt gctaactagg agggacatag atgctaaaag
    18061 cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt cgtacttttg
    18121 gatgtgctcg atttacctat aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa
    18181 caggagtagc caatttttcg ctcacacccg ctacattaaa aaaagaatac ccattcttaa
    18241 aagaagtaga cagtcttgct ttagcaaatg cacagcttaa tttggatcgg gcttttcgca
    18301 attatttcaa gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagt atgtggcaat
    18361 cttatacgac caacaaccaa acccgtacga tctatctgga aaatggctac ttaaagttac
    18421 caaaacaaaa agaactaatc aagataaatc agcatcggcc agttgaagga tcgattcgct
    18481 ccgctacgat ttctgctcga tataatgaag aattttatgt cgcactactt tgtgatgttt
    18541 cgccagtaaa aaaagaatct ttagctaaat ggatcgggat cgcctatcat ccgaagacat
    18601 tgatccaaac atctcgacct ctagaagtaa ctttaccaaa atttcaccag acagaagaaa
    18661 agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac catagaaaga
    18721 tcaggttaga tcaggcaagc aattatcaga aacaaaagag aaaagtaatg gatctttatc
    18781 tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt atctgggaaa ttggtcaaac
    18841 aatatgacta cttatttgta gaatcttttc caaaagaaga ggcacatgct gatttttcta
    18901 ttcacgactg gcataaatta ataacaaaat tgcgttataa gtcccaatgg tataataaaa
    18961 aatttctttt gatcaatacc gacggagcag aagaaagcaa ttccgtaaga aagagccaag
    19021 tagtagaaaa aatgggcaga cattcggtta tcaagggata aaaaatgaat gaaactgata
    19081 ggagcgttag ggttagcctt gttaataaaa agtcttaatg acttttgttt ctaggaattt
    19141 ttcttttgaa gaaaagctca ctaattatga caaaccaata aaacaatagc agaaaaagtt
    19201 cgtacgagct ttttctgcta ttgttaattt tttattcaga aagtttacga atacatcaaa
    19261 attctgaaac aaaaaacgta actcacaaag agttgttttt gcgttttttc ttgaatgatt
    19321 aggtctgaat cttttgctca caaccttatt catggtgccc cggcttgagc tgatagattt
    19381 ttgaaggacg gccgattcct acaggacgtt caccgatctc atcgaaatat tggagcatag
    19441 ctttcttaaa attggaatga tcgatcgttt tataatcgat ccctaagaat ttagcaaata
    19501 cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt
    19561 gttccatttt attgactaca cggttgaatg ctttcaagat gatttgacta tggtcaaaag
    19621 ccaaagtatc tttgccagaa gactctccag tgactaaatc aagcgagata gccacttctc
    19681 cgctagataa atggatcttg ttactatgac gttctagtgt gaaccagcgt acttccttgg
    19741 catcatctcc ggcgattagc ggttcttctc caataaaggc taagtagctg actgtgacga
    19801 cccagcctcg cggatctcga ttaggcgtac tgaatgtatg cagttgctca atattttctt
    19861 ttgaaataac cacaccagtt tcttcctttg tttctcttaa gacgctttct ccagttgact
    19921 catccttttg gacaaagcca ccaggcaatg cccatgaatt tcgataagga tgcccttttc
    19981 tttggatcaa caaaactttc agctgatctt cttctttgtt atagcacatc agtacgatat
    20041 cgacagtcaa agagggcttg tcatattctg gacgttcctg tttttgatac cagttaagaa
    20101 attcttcttc acttgcttta tgttcataat ataatttttc ttcttcttta gaagcaaaac
    20161 ggttcatctg accacctcca ttgacagtaa aagtactttt taccttattg tagcagactc
    20221 tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat catttaaata tgaaaaataa
    20281 atggaagtat gagattgaat aaaaagaaaa gcagataaaa cggcaatact ttataccgct
    20341 cttcacgaat ggcaaagaag ccacaaataa cgatatatac gtcaatgata aaacgcaaaa
    20401 tccaagctgc tgtcataaaa ctgttcaaat gcccatggag gttaaaataa ccatacatac
    20461 gaatataaaa atagctgaat attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa
    20521 aaaaaagatg tttcttctgc atgattgttc cagtccctta ctaagataga tagtctttat
    20581 tataccacgt gtgaggggag aaataaaaag ccgtttttta gtagatatga aacaaaacaa
    20641 aaacccgcat ttattcaacc tattcatcct tttaaaggat agattgaacg tagatagaag
    20701 tcgtttatcc agtctttttc attttgaaaa aaagctaaca gcatgctttt tttatggtat
    20761 actagcaagg aaacgaattc gcatcatgcg ataaaggagg aagagccccg tggcagaaaa
    20821 ggaaactttt tatatcacta cgccaatcta ttatccaagc ggcaaacttc atatcggcaa
    20881 ctcgtatact acgattgcat gtgacgcaat tgctcgttat aaacggttga tgggctttga
    20941 tgtgttttat ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga
    21001 actaggtgta aaaccgcaag aatatgtaga taaaatggcg gctgatgtca agaaattatg
    21061 gaaaacgctt gatatcagtt atgataaatt cattcgcacg acagatgatt accataaagc
    21121 agctgttcaa aaaatatttg atcgtttatt agaacaggga gatatttact taggtgaata
    21181 tgaaggctgg tactctgttt ctgacgaaga attctttaca gaaacacaac ttgcagaagt
    21241 gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga
    21301 attggtaaaa gaagaatcat acttcttccg catgagcaaa tacgcagacc gtctattgga
    21361 atactatgaa gaacatcccg aattcatcca gccagaatct cgtaaaaacg aaatgatcaa
    21421 caactttatc aaaccaggat tagaggatct tgcagtttca cgaacaactt ttacttgggg
    21481 agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg cattatccaa
    21541 ttatattact gcactaggat atggctccga agatgaaagt ctattcgaaa aatactggcc
    21601 tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt catacgatct attggccaat
    21661 catgctgatg gcccttgatt tgcctctacc taaaaaagta tttgggcatg gctggctatt
    21721 gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt
    21781 ggaacgttat ggtttagatg cactccgtta ttatttgctt cgtgctgttc cgtttgggtc
    21841 agatggtgtc tttacgccag aagattttgt ttcccgtgtc aattatgatc tggcaaatga
    21901 tctagggaac ttattgaatc gaacgattgc tatgatcaac aaatattgtg atggactcgt
    21961 tcctgactat gcctcacttg tcactccatt tgatagtgaa ctgtctacga cggctgctaa
    22021 cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa ttcaatacag cattggctga
    22081 aatctgggtg ctgatttctc gtgcaaacaa atacattgat gaaacagaac catgggtatt
    22141 agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc atgatccatt tagctgaatc
    22201 tttgcgtatc gcagcgatat tacttcagcc aatcatgaca gaaacgccag agaagatctt
    22261 caatcaacta ggattggact ctgaaacaat gaatcttgaa ggattacact ttggcgaatt
    22321 tccttcagga acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga
    22381 agaagaagtt gcttttattc aagaaaaaat gtctgaaggt actcaaacaa acgaagatac
    22441 agtcaaatgg gatccagaag aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt
    22501 tgatgtattt gaaaaagtag aactaaaagt agcagaagtc atcaactgtc aaaaagtaga
    22561 aggggcagat aagttgctgc aattccgact agatgctgga gacagccaag accgtcaaat
    22621 cttgtcagga atcgctgaat tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt
    22681 cattgtagca aatctgaaac cacgaaagat gcgtggacaa atcagtcaag gaatgatcct
    22741 ttctgcagaa gcaccagatg gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa
    22801 tggctcagaa atcgcataga aattcaaagg gggtatgaca ctagttgtgt cataccccct
    22861 ttttccgaaa tttgagaagc aattttcgta aattccttct tattacttga agccaatcat
    22921 ataatacaga aacagagaac tatccaaaat aaatcctaga aaaagaagta cgagcttcgc
    22981 tgatttttgt taatttagaa taatactaat aggaatcgta aaaacagaaa aaaagcataa
    23041 tgataaaggg tcgggcagat tttaattgat tcatctggta ctctttttta ttgtccttac
    23101 attcataaag tttgaatgca atgactttat tacatctata tttatatcta acagtagcgt
    23161 tgatgtgtta attataagtt ctatcacttt atgttcaatt agtttgtgct cttatatgtg
    23221 tgattgaaaa tatagataaa aaaacaatca tcggctttga cttcgatgat tgttttcttt
    23281 attttgtaaa aatcctatca cttgatttta tggtaacctt ttatttttgt cttcaaacgg
    23341 cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa agacatacca gaaaatcgta
    23401 cgtccgatac cataatgttg atagagcttt gtcaacatca cgtgtaacaa gatcaaccct
    23461 aaaagcaagg agccagctat aaaccaatga ttatcccaat gcatgacttc actaagtgcc
    23521 atcactccaa aaggatagaa ataaaaatag atcccgacta aggtcatccg ccagttcttt
    23581 gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga
    23641 aaaagcccaa ccaaaaagga atacaacatc ccccacaaga tcatcacagg acctttgatc
    23701 aatgcaacga ttgcgatgaa caaagtcagc aatattagtt tttgtgataa cgttgcaaca
    23761 gtcattaatc caaataaaat taatgtgaac caatgccaat gggactgctg ttgttcttta
    23821 cgggaaaaat cgacccggtt ggataaataa tctgctaatt tcggataaga acgcttaaaa
    23881 tccataaact gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga
    23941 aatacatccg tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt
    24001 aaaatattct cagttatact gaaaattata ccaaatttca aagaagaaag aggatgggaa
    24061 tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc attagctacc gttatgggaa
    24121 ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt actcagtcta
    24181 caagtttgac attgctagct tggcttttgg gtggtttctt aacgatctgt gctggattga
    24241 cagtggctga attagctaca gcaatcccgg aaactggtgg agctgtaaaa tatattgaag
    24301 ctgcgtatgg aaaattgccc agctttttat taggatgggc gcaaagcttg atttactttc
    24361 ctgctaatat tgctgcatta tcgattatct ttgcgacaca attgacgaac ttattacaat
    24421 tatctaccga ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg acagggttga
    24481 atttgttagg gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt gttaaattaa
    24541 taccaattgc tgtgattgtc atatggggat tattgacccc aggacaagga actgtccagt
    24601 tatttccaat cgaagctgga aaagatgtta cttttgtcga aggattgagc agtgcgttgt
    24661 tagcaacgtt gtttgcttat gacggctggt taggtgtcgg tgcaatggca ggggaaatga
    24721 aacggccgga aaaagatttg cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg
    24781 tttatctatt gatcaatttt gtttttttga aaactttgcc gattgatcat cttgccggta
    24841 atttgaatgc agcttctgaa gcatcagacg tcatttttgg aggaatcggt ggaaagctgg
    24901 tcactattgg gatcttgatc tcggtatacg gtgcattgaa cggttataca ctaactggga
    24961 tccgcgtgcc ttatgcgatg gctttagaag acgagctacc tttcagtaaa cagctgacaa
    25021 agctttcaaa gaagtttacg gttccttatg ttcctgcggt tttccagtta gcagtagctt
    25081 gtattatgat gagccttggt tctttcgact ttttaacaga catgttgatt tttgtgatgt
    25141 ggctattcag tttactgatt tttatcggtg tctttatttt aagaaaaaaa gcgccggaat
    25201 tgccacgtcc atataaagta ccactttatc caatcgttcc gattattgcg attctcggtg
    25261 ctattttcat tttaggtatg acgatgatga cgcaaacgaa attagcattg atcggtatag
    25321 gtgttacctt gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt
    25381 aaaatatttc aacaacagac agaaaaagta cgaactaggt cctctagatc ttcgtgcttt
    25441 ttctatttca ataaaacaag ttcttctatt ttgatttttg ctacttctgc aagtagtgct
    25501 tatgtgctaa agtaagaatg aaaaggagag ataaacatga tatttgattc tcatacccat
    25561 ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc acaagaaatg
    25621 ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa
    25681 ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac agaggctggc
    25741 agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc aaaggttgta
    25801 gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga agtccaagat
    25861 aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat cagtattcat
    25921 actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt tcgagatatc
    25981 ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt tcttgactta
    26041 gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga cgtccaagag
    26101 gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc ttatctggca
    26161 ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac agtagaaaag
    26221 attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg gacaaatgcg
    26281 caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga ttatagtcgt
    26341 tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata cgatagaaac
    26401 aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc aagagactcg
    26461 tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa aaatcattat
    26521 ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta aaccaaagaa
    26581 aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag cattgaaaaa
    26641 ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac aagacctttt
    26701 agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga
    26761 gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat
    26821 tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg agtgaatatc
    26881 gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt ttttcattta
    26941 aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa atcgtagaaa
    27001 ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc ggtgcattga
    27061 cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga
    27121 tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta catcaagata
    27181 ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag cttccgttaa
    27241 aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat tttcttgaat
    27301 cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct
    27361 ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa tattatatgg
    27421 aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt
    27481 cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca gacgaaaaag
    27541 catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt tggaataact
    27601 tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa agcttggaaa
    27661 cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa ttcgccgcat
    27721 taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa
    27781 aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catgtttttc
    27841 gtttgtttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag aaagaggttg
    27901 tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    27961 tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat gccgaacaag
    28021 gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa aaaattctta
    28081 tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc ttgacagtta
    28141 ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag gagagagaaa
    28201 aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt gggatggggg
    28261 tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt actttggcgt
    28321 ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc
    28381 taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg cgagcattgt
    28441 cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg agtatcgttt
    28501 taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat tttgtgatcg
    28561 gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt attggtaata
    28621 tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg atggggattg
    28681 gtgggcttat ccacagttta gttgattata atattgctta ttttatagca ggaacactca
    28741 gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga
    28801 ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa aaaactctca
    28861 tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa agagctgcac
    28921 caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat gacttgttct
    28981 gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac tatttcgcct
    29041 ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc agtttttgtg
    29101 taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt cactacggct
    29161 tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg aataccgaag
    29221 ataacttctt ttccttcata acctttttca actaatactt tgttttttcc ttcaggaata
    29281 cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac attaaagaag
    29341 ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt atcataaact
    29401 tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat tcggtcagcc
    29461 atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc aagacgctgg
    29521 tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa atttgataat
    29581 ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt
    29641 tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt cagacctaag
    29701 atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt acgtaatttc
    29761 aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta gttttggaaa
    29821 accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc gccgatattt
    29881 agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt tgactttcca
    29941 caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag attaaaatca
    30001 gttacagaat aattttctgc attgtcatat tttttatata cgtttttcag agccatttct
    30061 accataatac gttccacact ttctttttta gttttattaa ctatttaaag tataaatgaa
    30121 agcgttaact aacttcaatg tcatgttgaa caagaatttt ttcttttttt tgtcaatgtg
    30181 ctagttttaa gctttaataa atatccataa gaaataaaaa ctgatacact ggagtaaata
    30241 agtatgttaa caatatcaaa aggagtgtct cttatgaaaa tcgctttgat cgctcatgac
    30301 cgaaaaaaac ctatgatggt gaaattagca acagcgtata aaacaatatt ggaaaaacat
    30361 gaactttttg ctaccgggac gactgggcag aggatcacgg aagcaaccgg cttgccgatc
    30421 catcggttta aatccggacc gttaggcggg gatcagcaaa ttggagccat gatttcggag
    30481 gatcagttag atttggtgat ttttcttcga gacccgctgg ctgcacagcc ccatgaacca
    30541 gatgtaacag cacttatccg tttatctgat gtctatgaga ttccgttagc tacgaatatc
    30601 ggaacagctg aggtgctact aagaggactg gaagccggct tcctagattt tcgaagaatc
    30661 gtccatgaaa tagacaagca tccactttca ttttgatgga taagcgtatg gaaaaataac
    30721 gtttaatgtt cgcctatatg tagctataaa agctaaacaa actgataata tgcgatataa
    30781 tacgaacgta ttggataata taaacaataa tattagtttt tcgtatttgc agtaaatgtg
    30841 aaaatatgct atagtttctg tgggaaaaga tcccaaaaat aatacagagt agaaaataaa
    30901 gcgttaagtg ctaagaggat gggatgttgc ctcttggacg aaggacgtaa tgttcgcggt
    30961 tttattttcg cattcgctgc atgatacgat gtagcagctc ttaaaggaga tgctaaaaat
    31021 gaagaaacac cggttagacg cacgtatgat tgctattatg ggcttattga ttgcattgat
    31081 ggtcactttg tcccgactgg tagcaatcga gaccccattt attaaaatca gtgtcacatt
    31141 tattccacaa gtgatcatgg gcattctttt tggtcctttt tggtcaggga taggcgcagt
    31201 tttagctgat ttagtaggaa tggccctctt ctcgaaatct gcattcttta ttggatttac
    31261 attgaatgca tttattgaag gagcaatcta cggattcttt ttctatcgaa aagagatcac
    31321 ttggaaaaat gccattctgg ctacgcttag tgtcacgctg atcatcaact tatttttgac
    31381 accattatgg cttgcgttga tgtaccatgt gccattgttc agctgggtcg tttgggcgcc
    31441 ccgcctgtta aaaacagtaa tatggttacc gattcaatca attgccattt attacgtagg
    31501 acgtagtatt ccttacaaaa aaattttaag aagtttagcg atccacgcta aataaaaaac
    31561 aagcagtctt cccatcccag aagactgctt gtttttattg tgctgtgaat tgtgcttgat
    31621 agagccgtcg gtaataacca tctttttgaa gcagctcatc atgggtgccc atttcgacaa
    31681 tagctccctg atccatcact aatatctggt ccgcattttt gattgtggac aggcgatggg
    31741 caatcacaaa actggttttt ccttccatca ttgctaagaa ggcttgttgg atttttttct
    31801 ctgtcaatgt atcgactgag ctggtagctt catctaagat caacataggg ggattactga
    31861 tcatcgttcg agcaatcgtc aacagttggc gttgtccatc tgatatcttt aacccttgac
    31921 cgccaatctt agtatctaat ttttccggca aacgttctac aaattcatac atataagagg
    31981 cttttagggc atcattgatt tctgcgtcac ttgcttccgg attcccaaaa cgaagatttt
    32041 ctcgcaatgt actgtcaaat agccaagtat cttgtaacac cattccaaaa cttttacgta
    32101 atgtatcacg ttggatagag cggatgtcat ggtcatcgac tgtaattgtt ccttttgttg
    32161 tgtcataaaa acgcatcagc aaattaacaa gtgtggattt tccggcccct gtttttccta
    32221 ctatagcaat ggtttgacct ggataagcag taaaattgaa atcttcgatc aaacgctgct
    32281 tggggtcata agaaaaagaa acatgatcaa aagagacttt tcctttgata tttttgggtt
    32341 ccaaggcata ggcatctggc aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg
    32401 ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt gattccagaa agttcgataa
    32461 atggtttaga aaactgactt gagtagatcg taaagctgga aatcacacca acagtgattg
    32521 acgaaccact cttcaatgcc aataggccgc caactaaacc aatcgataaa taagcaaggt
    32581 gatctacaaa acgagaagac gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt
    32641 tttcatatag atcagcattt agcagttcaa attgctgttg actagcttct tcttgctgaa
    32701 aagctttcac gattttctgg ttaccgattc gttcggtcac aaagcccgag atatccccaa
    32761 taatcctttg ctgtctatcg aaatttgttt gtgaagcacg agcaacgatc caactcacaa
    32821 ggaaaataat tggtgtagaa caaagaacga ccaatgtcag aagtggactt agacgcatca
    32881 taaaaataaa cgaaagaatg atgattgcca ctccagaaaa taattgattg aaggccgcag
    32941 aaattgcaat ggaaatattg tccatatcat ttgtaaatcg actgacgata tttccgtggg
    33001 agttttgatc ataataacga agtggcaatc gattcagatg agcaaatgca tcttttctta
    33061 attttgcaac ggataagtaa gctaaccgat tgcttaacac ttggatgagc cattgactga
    33121 ctacgttgat taaaacgata ccaccgaata gaataagtaa atggaacagt cgattgaagc
    33181 ggacctgtcc ttctccaatc atttgatcga tactttgacc aatttgtaac gtcatcacaa
    33241 ctgttgcaat cccattgatg attcctagta tcacagctag ccaaatgtct tttgcatagg
    33301 cagtaaggta aggaatgaat cggcggagcg tctgcaagga taattttttt actttcataa
    33361 ctattcttcc tcctgtgatg cgacgatttc ctgatattct gctgattgag caagtaattc
    33421 ttcatgggta ccctgagcag caatcttacc ttgatccatc actaagatat gatgtgcttc
    33481 ttgaatagag cggacacgtt gggagataat aatcactgta ctgccaagat aagtctgtag
    33541 cgccttacgc aaatccaagt ctgtttgata atccaacgca cttaaagagt catctaaaat
    33601 cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc
    33661 agagaaattt ttcccatttt ccatgacttt tgtatctaaa ccatttggaa gctgacggac
    33721 aaattcttct gcctgagcga ttttcagagc ttcccaacat tctttttctg tggcgtcttt
    33781 cttaccccat tgaaggtttt cacgaatagt cccggtaaat agtacagaag tttggggaac
    33841 aagagcaatt tgctgacgga gatgatccag ctgccaagaa cgaacatcca ttccgtccat
    33901 tataactgta ccggaactga catcatagaa acgcggaatc aattgtgtca aagtcgtttt
    33961 cccgcttccg gttggaccaa tgattccaag gactgtattt cgcttcagct gaaatgtaat
    34021 attttggaga gaaagaccag aatcaggagt ataacggaag tcaacatggt caaaagcgat
    34081 aattgcttca gaagaaaaat cgggagtagt actgacctca gcttcaagaa tcgaattttc
    34141 tgtgtctagc acctctttca cccgatttcc tgaagcttcg gcacgtgtga agatcacgac
    34201 gaggtttgaa acaacgatca gtgccaaaag catttgattc atataattga tcaaagcaag
    34261 aacttgtcct tgttgcaagc taccaatatt tactttgatc ccgccaactg tcagcaaaca
    34321 gatgatcccg acattcatga tcaaggttgt agcaggtgat aatagggcag agatattgga
    34381 aacacgtaaa taattgttcg ctagatcatt tgtgctttta tcgaacttct ctgtttcttt
    34441 ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta agattctgac taacgtgttc
    34501 attgagctga tctagttttt cttgcacttt ttggtagaga ggaacggttt ttttgatgat
    34561 aaaaaataaa atgattgaga aaataggcaa taatgctaag aaaaacagac ctacttccca
    34621 gtcgatcaca aaagccatga ccactgaccc gatacttaaa aacggtgctc tgatcaataa
    34681 acgaatgacc attgctaaag ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat
    34741 caaggtatct gttccaaaat gattcaactc gttgtgagag aaagtattga tttttttgat
    34801 tagctgatta cgtaactctg taccaaatcc ttgtgaagct actgaagcat agtattggca
    34861 aatgatagca caaatcagac caatcacaga catagcgacc atccataagc ccatttcaat
    34921 gatttttcct cggtcattca tttttagtcc attatcgatc aaggaagcca tcattaaagg
    34981 caggacaagt tcgaagacag cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt
    35041 acgatagtct tttgcataac gtaataactg aatcatatga ttcctcctat tatttatttg
    35101 tccacaattg aaaaaacaag tactaccaca ttgtagctta ggaaatttag aatgaaaaga
    35161 gaacaagcct tttcataaat gaaagtttta ttatgaagaa gctttagaaa ttgaatagac
    35221 tatttttttt aaaaa
//
LOCUS       DASJTM010000023        33038 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJTM010000023 DASJTM010000000
VERSION     DASJTM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.023"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..207)
                     /locus_tag="U4B29_001018"
     CDS             complement(<1..207)
                     /locus_tag="U4B29_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105982.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTN"
     gene            394..861
                     /locus_tag="U4B29_001019"
     CDS             394..861
                     /locus_tag="U4B29_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105983.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            858..1100
                     /locus_tag="U4B29_001020"
     CDS             858..1100
                     /locus_tag="U4B29_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN2105984.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            1118..1420
                     /locus_tag="U4B29_001021"
     CDS             1118..1420
                     /locus_tag="U4B29_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105985.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1492..3546
                     /locus_tag="U4B29_001022"
     CDS             1492..3546
                     /locus_tag="U4B29_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105986.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            3546..6158
                     /locus_tag="U4B29_001023"
     CDS             3546..6158
                     /locus_tag="U4B29_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN2105987.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            6155..8686
                     /locus_tag="U4B29_001024"
     CDS             6155..8686
                     /locus_tag="U4B29_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105988.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            8738..9070
                     /locus_tag="U4B29_001025"
     CDS             8738..9070
                     /locus_tag="U4B29_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105989.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            9071..9664
                     /locus_tag="U4B29_001026"
     CDS             9071..9664
                     /locus_tag="U4B29_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105990.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9827..11521
                     /locus_tag="U4B29_001027"
     CDS             9827..11521
                     /locus_tag="U4B29_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN2105991.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            12209..14086
                     /gene="ltrA"
                     /locus_tag="U4B29_001028"
     CDS             12209..14086
                     /gene="ltrA"
                     /locus_tag="U4B29_001028"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2105992.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            14217..14594
                     /locus_tag="U4B29_001029"
                     /pseudo
     CDS             14217..14594
                     /locus_tag="U4B29_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            14594..15799
                     /locus_tag="U4B29_001030"
     CDS             14594..15799
                     /locus_tag="U4B29_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2105993.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            15815..16459
                     /locus_tag="U4B29_001031"
     CDS             15815..16459
                     /locus_tag="U4B29_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105994.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            16470..17276
                     /locus_tag="U4B29_001032"
     CDS             16470..17276
                     /locus_tag="U4B29_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105995.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            17298..17528
                     /locus_tag="U4B29_001033"
     CDS             17298..17528
                     /locus_tag="U4B29_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105996.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            18074..18349
                     /locus_tag="U4B29_001034"
     CDS             18074..18349
                     /locus_tag="U4B29_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105997.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            18391..18813
                     /locus_tag="U4B29_001035"
     CDS             18391..18813
                     /locus_tag="U4B29_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105998.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            18832..20424
                     /locus_tag="U4B29_001036"
     CDS             18832..20424
                     /locus_tag="U4B29_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2105999.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            20436..20771
                     /locus_tag="U4B29_001037"
     CDS             20436..20771
                     /locus_tag="U4B29_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106000.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            20918..21112
                     /locus_tag="U4B29_001038"
     CDS             20918..21112
                     /locus_tag="U4B29_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106001.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            21144..23516
                     /locus_tag="U4B29_001039"
     CDS             21144..23516
                     /locus_tag="U4B29_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106002.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            23577..25037
                     /locus_tag="U4B29_001040"
     CDS             23577..25037
                     /locus_tag="U4B29_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN2106003.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            25048..27252
                     /gene="topB"
                     /locus_tag="U4B29_001041"
     CDS             25048..27252
                     /gene="topB"
                     /locus_tag="U4B29_001041"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2106004.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            27268..27417
                     /locus_tag="U4B29_001042"
     CDS             27268..27417
                     /locus_tag="U4B29_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106005.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            27491..28084
                     /locus_tag="U4B29_001043"
     CDS             27491..28084
                     /locus_tag="U4B29_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN2106006.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            28100..28999
                     /locus_tag="U4B29_001044"
     CDS             28100..28999
                     /locus_tag="U4B29_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN2106007.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            29002..29487
                     /gene="ssb"
                     /locus_tag="U4B29_001045"
     CDS             29002..29487
                     /gene="ssb"
                     /locus_tag="U4B29_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2106008.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            29853..30113
                     /locus_tag="U4B29_001046"
     CDS             29853..30113
                     /locus_tag="U4B29_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106009.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            30554..30760
                     /locus_tag="U4B29_001047"
     CDS             30554..30760
                     /locus_tag="U4B29_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN2106010.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            30760..31011
                     /locus_tag="U4B29_001048"
     CDS             30760..31011
                     /locus_tag="U4B29_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106011.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            31026..31463
                     /locus_tag="U4B29_001049"
     CDS             31026..31463
                     /locus_tag="U4B29_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106012.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            31456..32163
                     /locus_tag="U4B29_001050"
     CDS             31456..32163
                     /locus_tag="U4B29_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106013.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            32544..32723
                     /locus_tag="U4B29_001051"
     CDS             32544..32723
                     /locus_tag="U4B29_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106014.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(32863..>33038)
                     /locus_tag="U4B29_001052"
     CDS             complement(32863..>33038)
                     /locus_tag="U4B29_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2106015.1"
                     /translation="FNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGF
                     QQVTDTLVSMFTE"
BASE COUNT        12343 a         5255 c         6809 g         8631 t
ORIGIN      
        1 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
       61 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
      121 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
      181 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
      241 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
      301 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
      361 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
      421 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
      481 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
      541 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
      601 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
      661 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
      721 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
      781 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
      841 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
      901 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
      961 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
     1021 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
     1081 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
     1141 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
     1201 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
     1261 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
     1321 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
     1381 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
     1441 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
     1501 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
     1561 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
     1621 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
     1681 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
     1741 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
     1801 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
     1861 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
     1921 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
     1981 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
     2041 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
     2101 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
     2161 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
     2221 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
     2281 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
     2341 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
     2401 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
     2461 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
     2521 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
     2581 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
     2641 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
     2701 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
     2761 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
     2821 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
     2881 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
     2941 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
     3001 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
     3061 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
     3121 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
     3181 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
     3241 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
     3301 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
     3361 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
     3421 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
     3481 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
     3541 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
     3601 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
     3661 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
     3721 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
     3781 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
     3841 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
     3901 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
     3961 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
     4021 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
     4081 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
     4141 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
     4201 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
     4261 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
     4321 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
     4381 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
     4441 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
     4501 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
     4561 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
     4621 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
     4681 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
     4741 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
     4801 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
     4861 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
     4921 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
     4981 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
     5041 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
     5101 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
     5161 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
     5221 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
     5281 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
     5341 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
     5401 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
     5461 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
     5521 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
     5581 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
     5641 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
     5701 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
     5761 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
     5821 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
     5881 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
     5941 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
     6001 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
     6061 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
     6121 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
     6181 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
     6241 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
     6301 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
     6361 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
     6421 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
     6481 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
     6541 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
     6601 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
     6661 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
     6721 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
     6781 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
     6841 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
     6901 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
     6961 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
     7021 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
     7081 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
     7141 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
     7201 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
     7261 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
     7321 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
     7381 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
     7441 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
     7501 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
     7561 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
     7621 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
     7681 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
     7741 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
     7801 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
     7861 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
     7921 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
     7981 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
     8041 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
     8101 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
     8161 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
     8221 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
     8281 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
     8341 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
     8401 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
     8461 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
     8521 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
     8581 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
     8641 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
     8701 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
     8761 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
     8821 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
     8881 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
     8941 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
     9001 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
     9061 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
     9121 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
     9181 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
     9241 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
     9301 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
     9361 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
     9421 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
     9481 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
     9541 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
     9601 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
     9661 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
     9721 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
     9781 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
     9841 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
     9901 aaatcgggaa atagaaattg taaaatccaa agggtatgat ttagattttt taaaacaagt
     9961 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    10021 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    10081 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    10141 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    10201 agcgactgac cgaaataaaa gcgcggaaga atacctggaa ctagcggaat atgctagaaa
    10261 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    10321 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctca aagggatgga
    10381 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    10441 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    10501 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    10561 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    10621 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    10681 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    10741 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    10801 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    10861 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    10921 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    10981 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    11041 ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    11101 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    11161 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    11221 gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    11281 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    11341 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    11401 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    11461 tgagtgccat aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta
    11521 aaaactattg taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg
    11581 cttccactga tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct
    11641 cggtgaagtc ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc
    11701 tctagaggta aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt
    11761 atgtacaaac gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa
    11821 tgggagagac agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg
    11881 ttgttatgaa agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc
    11941 atctaagtta aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag
    12001 tgcatactcg atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga
    12061 tggtgaaagt agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg
    12121 attcgtctgt aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg
    12181 tcacgagcca attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa
    12241 gttgcggcat gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag
    12301 tttgaaggga gataatttca agaatctaat gcagattatc atttctgata acaacattgt
    12361 cctagcatat cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt
    12421 aacgattaaa gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg
    12481 attcgcacat tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa
    12541 attaagaccg ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca
    12601 agtattagaa ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa
    12661 tagatcagca gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga
    12721 atatgtggta gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat
    12781 gagacaaatc tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat
    12841 gttaagagca cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc
    12901 acaaggcgga atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg
    12961 gatagcaaat cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa
    13021 tggagcttgg gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga
    13081 aatataccta gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc
    13141 gaaaaaagtt ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc
    13201 tgaagaaaag tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac
    13261 gatgaaatta gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc
    13321 acaaagcatg attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa
    13381 tagcaaagat acctttatct atattggagt ctataacagt atggttattg ggattcataa
    13441 ttacttcaaa attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag
    13501 accaagtttt gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa
    13561 gtacgaagga tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc
    13621 aatattacca gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa
    13681 taaatacacc gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg
    13741 gaagatatcg tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc
    13801 ggacaatcga atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga
    13861 gcttatctta tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga
    13921 tagctatgaa aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca
    13981 aagtgaaacg attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa
    14041 actcaatgag ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg
    14101 acgggaaaac agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg
    14161 tacggtgtgg actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga
    14221 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
    14281 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    14341 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    14401 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt aaatcaatga
    14461 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    14521 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    14581 atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg ttaagcgtcg
    14641 tgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    14701 gatcactgtg ttcgtgaccc tatttctaat acttgcggcg ggctcgattg ataattcgga
    14761 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
    14821 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    14881 tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttacgaatc
    14941 agcgtttggt acatcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    15001 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
    15061 gtacatgaag tttcccaact caaaagctgc attttcctat tacggcttta tggtagccac
    15121 acaatccaat tttaatgctt gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    15181 aggacgaggc ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggttg
    15241 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    15301 gggtgaaggt ggcaataaca acggcacgat tactggtgaa tggacgaatc cattccctgg
    15361 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    15421 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    15481 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    15541 aggtttgggt gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    15601 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    15661 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    15721 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    15781 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    15841 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    15901 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    15961 gctcaaaaaa aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
    16021 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    16081 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    16141 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    16201 caaattaagt cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac
    16261 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    16321 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    16381 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcatagactt
    16441 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    16501 tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattctc ggctttttga
    16561 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    16621 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    16681 aattagtttt tgaatttgcg atcgatgatg aagatcaaag cttgttaaaa gaactggcta
    16741 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    16801 aagtgattaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    16861 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    16921 aaccacaaga tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg
    16981 aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    17041 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    17101 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    17161 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    17221 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    17281 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    17341 agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    17401 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatacct
    17461 taaaattcag atcatttgaa gctctatatg tagctgctaa aaaatatttt acagctgata
    17521 acaaataaaa aacaggagta ttttatgtga aaaaagtata taaaaaatgc ttatgaatca
    17581 gctatatcac cttaaccatt tgttaatgag atgggagaaa acattaacca cgattctaac
    17641 gcaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
    17701 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
    17761 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
    17821 ataaccttaa tttttaaaga gcataaggaa acgtgatatc actttctcac gaaaggtgtt
    17881 aggtaggctg tgcctaccac gtttctaaga aagataaatg atatcaaaat tttggatttg
    17941 tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag taaaataagc gtttgtgtgt
    18001 agcaaaatca aaaaatgtgt agggatatgt gtagctacac atatccctac acataataaa
    18061 tttggaggaa aatatggact tattaattag aaatattgaa gaaaagtata ttaataaaat
    18121 tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa aaatggagtc gaaatcaata
    18181 cctaaaagtt ttgatcgaaa atgattttga tcacgctctt ctgaattata aaaaagacca
    18241 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaactttaga
    18301 agaatatatt gcaacaaata atcaattaat tggtttgtta attgaataat aaaaatagta
    18361 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    18421 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    18481 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    18541 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgaa
    18601 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    18661 tcaactgttt atcaagaagc agaagaacgt gtggaaggaa aaattaaatc caatcaaacc
    18721 aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat ctgttcatac cagagtagat
    18781 ccatttgatc caacgaagat tctaaaaaga taaaagtaga aggagaacgt gatgaataaa
    18841 tcgcctgcag ttattcttac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    18901 tacattgatt atatgacaag agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa
    18961 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    19021 gaaacttttt cggaaagtaa gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa
    19081 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    19141 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    19201 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    19261 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    19321 gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc aatcatttat
    19381 caagatgtta tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    19441 acagatacct tagatgaaga aggtttgaaa gcagctggaa gaaaaatgat ggagacatta
    19501 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    19561 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact
    19621 gagattgaaa atggtgtgag ttatatcgta ccgaagggga agagaagaca agcaacatta
    19681 gacgcaatga aaaccaatta tgtacatgag ttagaacgtt tctcttatga aaaggatcga
    19741 atgatcggta tggagaaacg aaatttattg acacgaaaaa gtgatttgcg taacacctta
    19801 acaaaagtag taaaagaacc agcacgttac gatcgacacg ccatgaactt attaaatgaa
    19861 gtatataaaa ccctacctga aaaacgttcg gaatggaatt atggagatga aaatcgaacc
    19921 aagctttcac catttacaag gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa
    19981 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agccaaaagc
    20041 ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa aaaacgccct ctttgattta
    20101 aaaaagcgca caggaaatgc gattttaaca gaactgaaga gacaggatag tcaaataaaa
    20161 ccattaatag aaagtgtcaa tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    20221 ctcaaacagt tgccaacaaa tgaatacaag tatagttatc aagaattcgt aaagcgtagg
    20281 aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt
    20341 aatgcacatg aagaaaaata ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc
    20401 caaatggaac aagaaagaca ataggaggaa gagaaatgaa acaatattta gatcaatgga
    20461 aagtcataga aggatcacta aaagaagaac gaatcgaaca acttcctgat tgtttagaaa
    20521 aagagcattt gtttcagata agcgaaatgt tgagaaacga acagtttgat ccaaatcaat
    20581 ttttagttgt tgagtaccca gctactggtg tgtattgttg taatcatgtg aagggtgaaa
    20641 aatattttat tattcaagaa tacgaaggta agttagcgcc ttattatact acttgggaaa
    20701 taaatgaaga gggaatcaat aatttcccat gtgaaagcat tgaagagtct atttctctca
    20761 cggaatgtta aataaataga gacgtaaaag agtcctgaac gtgattgtcc aggactcttt
    20821 ttcaatgaat tgttttccgc caattcactg agtggtgcga ttcaatttta ttatatcaaa
    20881 agaaagtgag attccaaagt gaagggagga agaagaaatg actcagacaa cgattggtgt
    20941 tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca gaaattcaaa caggtagagc
    21001 ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa
    21061 tctgactacg aagatgttcg ttgatgcagt agaaaattat ccatttgttt agacaaacga
    21121 tattttacaa ggaggaaacg gacatggcag aagaaaaaga accactagat aatagtcggt
    21181 tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga attaaataat caatttcaaa
    21241 agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc
    21301 gttcatggtt taaccagcaa gaaaaaatag aaaataaaat acaaagtttg agagaagaga
    21361 tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg
    21421 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
    21481 aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc
    21541 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
    21601 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
    21661 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
    21721 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
    21781 aattacttga agaggctgca ccaactgatt ctctgaagga gaaagaaaaa gatcatttct
    21841 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
    21901 tcgtaacatt agaattgatc gatcaactta aaacgttgga taaccaatac gctgcagaag
    21961 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagta gatcagatcc
    22021 gaatggatct aggggatggc gtcaacagta atcaccccat ctatttagag ttagcaaaat
    22081 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
    22141 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
    22201 cagaagagat caaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
    22261 agcataccca ttcttctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
    22321 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
    22381 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
    22441 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
    22501 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
    22561 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
    22621 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
    22681 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
    22741 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
    22801 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
    22861 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
    22921 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
    22981 gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
    23041 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
    23101 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
    23161 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
    23221 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
    23281 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
    23341 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
    23401 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
    23461 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
    23521 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
    23581 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
    23641 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
    23701 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
    23761 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
    23821 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
    23881 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
    23941 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
    24001 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
    24061 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
    24121 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
    24181 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
    24241 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
    24301 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
    24361 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
    24421 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
    24481 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
    24541 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
    24601 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
    24661 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
    24721 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
    24781 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
    24841 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
    24901 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
    24961 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
    25021 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
    25081 caagcgaaag cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
    25141 atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc
    25201 gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg
    25261 cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt
    25321 gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt
    25381 gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc
    25441 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag
    25501 ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc
    25561 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
    25621 ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa
    25681 ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
    25741 atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa
    25801 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg
    25861 atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg
    25921 agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc
    25981 atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt
    26041 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa
    26101 gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
    26161 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
    26221 caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
    26281 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
    26341 ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag
    26401 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
    26461 gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacagcc ttataccgaa
    26521 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
    26581 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
    26641 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
    26701 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
    26761 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
    26821 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
    26881 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
    26941 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
    27001 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
    27061 aaacttacag cgacacagct gaaagagatc attcaaaata gccgaacaag ccaaccagtg
    27121 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
    27181 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
    27241 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
    27301 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
    27361 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
    27421 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
    27481 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
    27541 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
    27601 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
    27661 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
    27721 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
    27781 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
    27841 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
    27901 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
    27961 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
    28021 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
    28081 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
    28141 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
    28201 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
    28261 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
    28321 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
    28381 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
    28441 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
    28501 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
    28561 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
    28621 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
    28681 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
    28741 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
    28801 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
    28861 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
    28921 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
    28981 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
    29041 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
    29101 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
    29161 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
    29221 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
    29281 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
    29341 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
    29401 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
    29461 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
    29521 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
    29581 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
    29641 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
    29701 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
    29761 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
    29821 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
    29881 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
    29941 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
    30001 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
    30061 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
    30121 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
    30181 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
    30241 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
    30301 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
    30361 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
    30421 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
    30481 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
    30541 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
    30601 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
    30661 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
    30721 cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta
    30781 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
    30841 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
    30901 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
    30961 aacatggaaa cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
    31021 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
    31081 aaaaaatgga ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca
    31141 aggaaaaatt gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
    31201 ttgcgacgta ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc
    31261 aagccttact atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc
    31321 aagccttaga actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta
    31381 gcgactggct agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat
    31441 cggaggagaa agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa
    31501 tttttaacaa gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt
    31561 tgtatggaag aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc
    31621 gaaacaagtc aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt
    31681 cctgaaacgg taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg
    31741 aatgtgtacc tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg
    31801 aaacactatt actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa
    31861 gaagaaatgg aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt
    31921 actaggtttt tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc
    31981 gctgtacccg aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac
    32041 tggtacacga ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc
    32101 caaaatgaac ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa
    32161 taaccattta taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa
    32221 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    32281 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    32341 ttcctctatt gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac
    32401 atttaaactt cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg
    32461 tattagccct tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
    32521 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    32581 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    32641 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    32701 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    32761 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt gggagcgtca
    32821 ataattttgt gtaaataaat tgtcctcctg caaaataatt agttactcag taaacattga
    32881 aactaatgta tcggttacct gttgaaaacc tttatggctt ctgtttagaa atttttgatt
    32941 gtatgtatca aaaatgctga ctagaaagcg ttctagtgat tcttcatttt gaaactgctc
    33001 ttttctacgg ctgtatcttt taatttgctt attgaaag
//
LOCUS       DASJTM010000024        32662 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJTM010000024 DASJTM010000000
VERSION     DASJTM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.024"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="U4B29_001053"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="U4B29_001053"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN2106016.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="U4B29_001054"
     CDS             complement(1032..1952)
                     /locus_tag="U4B29_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2106017.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /gene="xth"
                     /locus_tag="U4B29_001055"
     CDS             2065..2817
                     /gene="xth"
                     /locus_tag="U4B29_001055"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN2106018.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3681..4214
                     /locus_tag="U4B29_001056"
     CDS             3681..4214
                     /locus_tag="U4B29_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN2106019.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4704..5228)
                     /locus_tag="U4B29_001057"
     CDS             complement(4704..5228)
                     /locus_tag="U4B29_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106020.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5221..5694)
                     /gene="tsaE"
                     /locus_tag="U4B29_001058"
     CDS             complement(5221..5694)
                     /gene="tsaE"
                     /locus_tag="U4B29_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN2106021.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5817..6797)
                     /gene="pta"
                     /locus_tag="U4B29_001059"
     CDS             complement(5817..6797)
                     /gene="pta"
                     /locus_tag="U4B29_001059"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN2106022.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6950..7630)
                     /locus_tag="U4B29_001060"
     CDS             complement(6950..7630)
                     /locus_tag="U4B29_001060"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2106023.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7756..8625
                     /locus_tag="U4B29_001061"
     CDS             7756..8625
                     /locus_tag="U4B29_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2106024.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8651..9166
                     /locus_tag="U4B29_001062"
     CDS             8651..9166
                     /locus_tag="U4B29_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106025.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9213..9806)
                     /locus_tag="U4B29_001063"
     CDS             complement(9213..9806)
                     /locus_tag="U4B29_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106026.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9831..10010)
                     /locus_tag="U4B29_001064"
     CDS             complement(9831..10010)
                     /locus_tag="U4B29_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106027.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10087..10596)
                     /locus_tag="U4B29_001065"
     CDS             complement(10087..10596)
                     /locus_tag="U4B29_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106028.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10692..11072)
                     /locus_tag="U4B29_001066"
     CDS             complement(10692..11072)
                     /locus_tag="U4B29_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2106029.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11402..12151
                     /locus_tag="U4B29_001067"
     CDS             11402..12151
                     /locus_tag="U4B29_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN2106030.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12472..13689)
                     /locus_tag="U4B29_001068"
     CDS             complement(12472..13689)
                     /locus_tag="U4B29_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2106031.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13947..15224)
                     /locus_tag="U4B29_001069"
     CDS             complement(13947..15224)
                     /locus_tag="U4B29_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106032.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15303..15947)
                     /gene="pcp"
                     /locus_tag="U4B29_001070"
     CDS             complement(15303..15947)
                     /gene="pcp"
                     /locus_tag="U4B29_001070"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN2106033.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16146..17090)
                     /locus_tag="U4B29_001071"
     CDS             complement(16146..17090)
                     /locus_tag="U4B29_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN2106034.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17092..17778)
                     /locus_tag="U4B29_001072"
     CDS             complement(17092..17778)
                     /locus_tag="U4B29_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN2106035.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18252..18572
                     /locus_tag="U4B29_001073"
     CDS             18252..18572
                     /locus_tag="U4B29_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN2106036.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18726..19631
                     /locus_tag="U4B29_001074"
     CDS             18726..19631
                     /locus_tag="U4B29_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2106037.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19628..20401
                     /locus_tag="U4B29_001075"
     CDS             19628..20401
                     /locus_tag="U4B29_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2106038.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20582..22006)
                     /locus_tag="U4B29_001076"
     CDS             complement(20582..22006)
                     /locus_tag="U4B29_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106039.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22217..22663
                     /gene="nrdI"
                     /locus_tag="U4B29_001077"
     CDS             22217..22663
                     /gene="nrdI"
                     /locus_tag="U4B29_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN2106040.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22728..24710)
                     /locus_tag="U4B29_001078"
     CDS             complement(22728..24710)
                     /locus_tag="U4B29_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2106041.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24724..25620)
                     /gene="murQ"
                     /locus_tag="U4B29_001079"
     CDS             complement(24724..25620)
                     /gene="murQ"
                     /locus_tag="U4B29_001079"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN2106042.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25623..26684)
                     /locus_tag="U4B29_001080"
     CDS             complement(25623..26684)
                     /locus_tag="U4B29_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2106043.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(26895..27461)
                     /locus_tag="U4B29_001081"
     CDS             complement(26895..27461)
                     /locus_tag="U4B29_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2106044.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(27489..28991)
                     /gene="eat(A)"
                     /locus_tag="U4B29_001082"
     CDS             complement(27489..28991)
                     /gene="eat(A)"
                     /locus_tag="U4B29_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2106045.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29249..30370)
                     /locus_tag="U4B29_001083"
     CDS             complement(29249..30370)
                     /locus_tag="U4B29_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2106046.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30496..31437
                     /locus_tag="U4B29_001084"
     CDS             30496..31437
                     /locus_tag="U4B29_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2106047.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31587..32483)
                     /locus_tag="U4B29_001085"
     CDS             complement(31587..32483)
                     /locus_tag="U4B29_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2106048.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         9433 a         6785 c         5842 g        10602 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagatacaa gcttttgaac accgtttatt
     3061 aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac aaaaatagtt
     3121 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3181 attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca aacaaaaata
     3241 gttcctcata tttttcgttt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     3301 tttattgaga agttgtgaaa tcagcgtcct gacctgagta ttagagaaac aaatggcaaa
     3361 atagttcctc atattttgct attttttgag ctaataccga gggtcagctg attgaacacc
     3421 gtttagtgga gaagttgtga aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa
     3481 aatggttcct cacaactttg cataaatttg acttaatacc gagaagtcat gatccccatc
     3541 taactccaaa tcacaaggaa tgagttcaaa aactagcttt tctcaacaaa tattacataa
     3601 aaaattctat gttttttgtt aaatttattt gcttttttca aaagtatgtt acagtaattt
     3661 cccaaggagg ttttacagaa atgaattttg ttgccatgga ttttgaaact gccaatcatc
     3721 agccttatag tgcttgttct cttgcgctag tcatggtaaa aaatagccag atcgttgatg
     3781 aattctatac attgatacag cctgagactc cttttttctg gcgaaatgtc caaattcacg
     3841 gaatccatca agaagacgta cgcaatgcgc cgaaattccc agaagtctgg caaactatcc
     3901 aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt gatacaaagg
     3961 tcttagcggg atgcttagat tattatcatc ttgaacagcc taattatctt tctttatgta
     4021 cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg aacactgtgt
     4081 gcgaaaattt gaacatccaa ttagatcatc accatgatgc attggaagat agtcgtgcat
     4141 gtgcggaaat cctgctttat caagaaaaaa aattcgggac agatccattg aaaaaacttg
     4201 tgactataaa gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc
     4261 actccgagtc acaaggagca atttcaaaaa atagcttctc atgtttatta aatattgtga
     4321 ctggtgaccg aggagctggc aggagaacac cgtttattgg agaggttgtg aaaaagctgt
     4381 tctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
     4441 ggacttatta ccgcagatgc aagcttttgg acaccgttta ccaagaggtt gtgaaatcag
     4501 cgttttgacc tgagtattag agaaacaaac ggcaaaatag ttcctcatat tttgccgttt
     4561 tttgagctaa taccgaaggt cagctgattg aacaccgttt attcaaaaaa aatggactgc
     4621 gacacgactt ttgtcacagt cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt
     4681 ttctcactct tttcctattt cttctattca atcaaataac tcattttata tacatctaaa
     4741 aattcaccta gatctgttct tgcccctcta ggcatgaccg cttcgatttg gaagcccatt
     4801 tttcggtaaa ggtgtacggc tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa
     4861 agtacaccca tttccttcgc ccaactgatg atttcttcta gcataagagt gcctagtccc
     4921 attccccaat attcctgcag aatactgata ccgacttctc ccacgtgaga caaacgaaat
     4981 tgactgtctg ccttgacaga tgctgttcca ataatcgtcc cttcatagat tgcaagcagc
     5041 aataaattat tattgctttc gtatatataa tccaactctt cttctaaggt ttctggtggt
     5101 aacaggagtt ctgcttcatc caaaactaaa aattctgtct ccttattaac ttgtcccatc
     5161 atcaaaagaa tgtcttttgc atctcctggc actgcttccc gcaagatgat cccaacttct
     5221 tcactcatga ttttcagccc atttttctag aattcgctgt ttgaaggttt cactttgctc
     5281 cccgtatgag attagtttca cataccgctt ttctaaatct gtatcacttt tttctaaaat
     5341 cagttccaaa taatcctccg gcagtgcttc ttctaataga tttccccatt caacaacgga
     5401 aagcccatca ccttcaaaat attcatctaa ccctaaatct gctccgctag ctgctacccg
     5461 ataaatatcc atatgataca acggaatccg accttgacta tattctctaa taatcgtgta
     5521 tgtcggactt ttgatcattt gcgttatccc taatccttgg gcaattcctt tcgtcaacgt
     5581 tgttttaccc gcacctagat caccagtcaa tatcagattg tcacctggca ttgcggaatg
     5641 accaattgcc tctgcgaatt gttctgtcat tgttaatcct gatagttcga tcatttttca
     5701 tcttctcccg ctcttctttc tttttgccag tatatcgcgt aatgaagttt ttttaaagaa
     5761 aaaatgaact agtcaagaac agcagctgct gtttttggct agttcgcgtg tatttgttaa
     5821 tcaatcaatg tttgtgctgc tgtgataatc gataatttat atacatcttc ttcatttgct
     5881 ccacgagaca aatcagaaac cggtttgttc aagccttgta gaattggtcc gattgcttca
     5941 aagttaccga aacgctgagc aattttatag ccgatatttc ctgactgcaa ttctggaaat
     6001 acaaagactg ttgcttgacc agctacttct gaatctggcg ctttcagttg tgcaactgaa
     6061 ggaacataag aagcatcaaa ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt
     6121 ttagcgattt tagttgcttc aactactttg tctacttctg gcgatttcgc tgagcctttt
     6181 gtcgagaagc tcatcattgc tacttttggt tcgatatcga acaattctgc tgtttttgca
     6241 gaatctacgg cgatttcagc caattcttgc gcagttggat tcacgttgat cgcacaatcc
     6301 gaaaagacat atttttcttg atctcttcca cgtaccatga tcatcgctcc gcttgtccga
     6361 ctgatgccag gctttgtttt gatgatctga agtgctgggc gaacagtgtc gccagtcgaa
     6421 tggatcgctc cagagaccat tccgtctgca atccccatat acgtaagcat cgtaccaaag
     6481 taattcacat cttttaaaat cttttctgct tgttctttgg atgcctttcc atttctacgt
     6541 tcaacaaaag tatccaccat ttcatcccac ttatcgtaat tatcagggct gatgatcgta
     6601 aagctagatg ttttgattcc tctggcatgc gctgcatttc ttatttcttc ttcgttccct
     6661 actaaaatgg gttccacaag ttcttctgct ttcaaccggg cagctgcgcc taaaatacga
     6721 gcatcagtag cttccgggaa aacgattttg atcccacggc gaacaatttt aaatcttaaa
     6781 ctatcaaaca attccacaag atgacctcca gacgttttta tatgtattat catacactat
     6841 tccagaaaaa aagaacagtt tattttatct gtatcagtca aaaatgctgg aaaaattaag
     6901 tttttcacat acaaagcgaa ctgcactata acaggaaagc gctgttttat tacactgttt
     6961 caggcagttg ccaatcaatc ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg
     7021 agaagggttt ggaaccaaaa aagccacgac ttgcagataa cggactggga tgtggagatg
     7081 ttatgatgat atggcgactc ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc
     7141 acaagataaa cacgattggt ttttcccgtt catttaattt ttcaatgatc gtgtctgtta
     7201 atctttccca acctttcccg cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg
     7261 tgttgagtaa aaggactccc tgtttagccc atgacactag atttccatgt tgaacaggtg
     7321 cgattccaag atcatcataa agctctttat agatatttct taacgatggc gggatcttta
     7381 cgccaggttg cacagaaaaa gataagccat gagcttgatt ttctccatga taaggatctt
     7441 gtcctaaaat caccacttta acttcttcat aaggagtcaa ttccaatgct tcatagatat
     7501 gatacatatc tggatggatc ttttgggacg catattcttt ttttaaaaat tctcttagat
     7561 gcagataata atcttttgaa aattctgagg acaaaacttc ttgccaactg ttatgaatga
     7621 tttctttcaa aaactccacc tcctaataaa aagaataact cacattttct tcaatcacaa
     7681 gggacaatct tcaccctcag cacttgatta atatggtaaa ctaggaacaa gaagcagaaa
     7741 ggaacgttaa tcattatgat caaattaatt gcatcagata tggacggcac attattagat
     7801 gcccatatga gtgtttctcc tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt
     7861 gttgagttca tggtcgctac tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag
     7921 gtaggaatcg attgtgcgat gatcactttg aacggggcac aggtatttga taaagaagga
     7981 aattcattgt ttaccgtgtc gattccaaat aatcaagcaa tctctgtttt agatattcta
     8041 gatgctaacg gtatttacta tgaagtagca accaacgatg gtctttattc tgaaaatcaa
     8101 gcgaaacgaa ttgaaagttt tgcttccatg gtggctaccc atttacctca ccttacgtat
     8161 aaaatggcga ttgccatggc ctcagctaag ctggagttat tgcacatcac atacgtggac
     8221 agtattcgtg ctattttgga agaaaagaat ctggaagttt taaaaattat ttgttttcat
     8281 acagaagggc cagctgtatt aggtccagtt ggaaagcaga tttctctttt aggcgatcta
     8341 gccgttactt cctctggtca aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga
     8401 attgctgttg cccatgtcgc tcacgagcgt gggatttctt tagaagaagt catgacgatt
     8461 ggcgataact tcaatgatgt cagcatgctg caagtagcag gagtcagttt cgcaatggga
     8521 aatgcagaaa tcgaagtaaa agattatgcc aaatatacaa cggacacaaa ccttgaatct
     8581 ggcgtaggaa aagccatatt gcgcgcgatt gcagaagact tgtagtttta tctagaaaag
     8641 agggatatag atgtcagaat ttttcattca ggaaaagcag cttggacgtg caactcggac
     8701 aattgtcaaa aacgaaaaag gacattccct gtttttgatg gttggtcgct ggggaacaaa
     8761 gggcgatgca ttgtctcttt atgccatgaa tggcgatctc gttgctcaca tcaagcagat
     8821 aagcctgaca ttcggtacgc gttttgagct gtataaagag ttcgaaaagg tgggaaccat
     8881 gcagaaaata ttcaattggc ccggtgactt ttattatatt cgtagacttc actggacagt
     8941 ccaaggtgat atctataatc atcaatatca aatccatcat ttcaatcagc aaatcatgaa
     9001 aatggataaa gcaacacttt tttcaggaga ttactatgta ttggatgtct caaatgatga
     9061 agatgctcca gtttgtattt gtattgctgc ggttttagat tactggctct accgtaaaga
     9121 caaaagtcgc gataagcctg tttcttaccg acttaactta agttaaaaag atgtacctga
     9181 aatgcggaaa tacgcatttc aggtacatct ttttagatat tcttttgcat atgatcataa
     9241 agatgaccac tgatcatccg ctgaccgaca actttgaagt catggcgttc gtataatttt
     9301 ttagcctttg gatttttttc atccacactt agaccgatga cggagcgatt agctttcttc
     9361 gccaatttag gtaatgcttc taataatcgc gaaccgatgc cttgtcctct aaaatcttct
     9421 gatacactga tagagtccaa ataccattcg ttgggaaacg tttctttatc aatgaacaaa
     9481 cgtgtagacg catctagctg atatttctct aagataggta ccagtggctg atcgatgatt
     9541 gattcttctt catctgtata accgtaagca actcctgcaa ctttaccgtc tatttcttct
     9601 acgatacctc gtgaataact gtaacgataa tttggatcag tgattgcttc ttctaaaatc
     9661 tctaaagtct tttgttcccc tgtctgcttc aaaaatggca gttccatatc ttttagaatc
     9721 actaagatca atggggcgat ttctggagca tcttcttttc tcgcaaaacg aatcatagtt
     9781 tcctctcctt ttctccattc taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa
     9841 aaatcttcaa tcttttcagt aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt
     9901 cggcaataag ggcagacgac tggcatttct ttatcattat gttcgactac attattgact
     9961 tcttcgtatg caaatttttt tccgcaatta gggcatttca ttttttccat gttatctcaa
    10021 cttcctttca tctttactct cttagcttac tcttatattg taaaaaagag aagcaatatc
    10081 cttcttctat gcagatatcc atacctgacg gtcctgttct ccatctatta atcggacttt
    10141 ataataagga cgttcatctt tgactagaag tgtccactct ttcacttttg cttttttccc
    10201 gtagtagctg actgcttcct tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc
    10261 tatatcaaaa agatgttctg cggccttttc cgtgtcattt cgtacggttg ttttcccagt
    10321 ggatggattg actgctatga cttgtccttc acaaaaaaac tggtattcat atgttgcatt
    10381 cgtaggattc tccaaatctt tttcaactgc aaaatgaata gcagatatgt tatgttcttt
    10441 tgtttcgttt tttacgattg ctgctgcttt tttcaatgag atccgatatt cctggtcact
    10501 aatatcatcc atgtgaaaag cggcattgag cagtcccgtc ataccggtaa tccctattac
    10561 gatgagggca gcaacaccaa acatcatttt tttcattctt cattctcctg tttctttttt
    10621 ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa
    10681 ttttgttatc tttattcgtg cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc
    10741 attttttttc ctgtaaaggt atcttctcta atcggttctg attcgatccc taaggactgc
    10801 aattctgcaa ttgtttcttc aatatcgtct actttgaaag caagatgacg caagccacaa
    10861 gcttctggat agtttggtcg ttgtggcgca ttagcaattc caaaaatctc caattcagca
    10921 cttcctagtt tcagatctaa tttatagtca tctcgttctt tgcggtaatt ttctctgatt
    10981 acttcaaatc ctaatttatt aacataaaaa tcacgtgatt tctgataatc tgaaacaatg
    11041 atggctatat gatggatcgt tgtaaggttc atatgagcac tcctattctt cgtttaagct
    11101 cggatctata ttaatctcca gttccattgt aaatgtgtca gattgaaaaa aatagctgtt
    11161 tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca agacactctt
    11221 cctgttccca atataataaa tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa
    11281 attatcataa cacaaattat ttataattga aagactagtt ttttttcaag aaaagcatac
    11341 aatagattgt gttagtaaat acagaacaca ctacatttag tgtcttaagg agtcctcata
    11401 catgaagtca tattttcagt ttttagctca tcagctaaaa ggctggccgc aacaaaatta
    11461 ttatcttttc tttttcagtc ttggctgcca ggtcatgact cttgtcaata gtcccatcac
    11521 ttggctgtcc gttattacat ttatcggtac cacgcttggt gtattgtgta ttttatccat
    11581 caatgcggca aaatcagtca acggtatttt aggtattttg tctgcgattt gttttattat
    11641 cgttgggttt tcagcaaaga actatttaag tatcggtgaa cagattgctt atatgatcac
    11701 attggatatc cccgtattgt taagtaaaga atggaatcat aatatggctt caaaaatccg
    11761 aaaattcact ggaaaaagct ggtttatcgc aatcatcagt actttccttg tctatcttgt
    11821 ttcaggattc ctgattggtc gttatacaga tgatcctcgg ccgtgggtag atgccattgc
    11881 tttttctatt agcttgactg ctggtatcat cagtttcttg cgttacaaca accaatatta
    11941 ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg
    12001 atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc
    12061 atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga
    12121 agaatactac aacgcacaac aacacgcata aaaaaactga gcgtgggaca aaagtaaaga
    12181 acacttttgt ctcacgctct aaacacgtat aaacggcgga atcagaagca actccttcgg
    12241 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    12301 tggagttaaa cgcttctgtc ccagcctcta tttcctacga ttagtcaagt aaaaggtgca
    12361 agaaaaatag ctcgtaaacg aaagcgacaa aatcaaagca caccaaataa agccccgttc
    12421 gtaaaaaatt ttcgatctgg gcaaagaagt ggtatactgt aagtggttta gttaatggtc
    12481 cactgagacc gcgtacgcat agtcgtactc tgtatgtgcc ctaaccatag cgtgcataca
    12541 tttcagaagc ttgttcatac aagcaactgt ggcaacctta tctttcttag ggataggttg
    12601 cttttttaat ttgtagtaat aatcgacaat atgattagga gctgcgcgtt gctggcgaat
    12661 catatttcga atggtgaaga aaataatttt ccgtgctttg ggattaccac gcttattgat
    12721 gcggtcttgg ccagtatact tacctgaatg ataccgtcgg atgtcgattc cgacaaaggc
    12781 attcaattgt ttatgggttt taaatcgtgt gatatctcct aattcaccga gcaataaagc
    12841 ggctgtgagt gcgccaatcc cagggaacgt ttggtacaac aaaaagcaag gagagccttc
    12901 ggcttttttg atcatttgtg tcgccagtgc ttttttttgt tcgagtaaat cttggagaag
    12961 ttcggcgtaa tagatcgttt tttgacagtg gatactgtct ttttcaacag ctggataggc
    13021 acactgtgca tagtggatga tttgatctgc cttttgctcg gcacgattcg cggagatttt
    13081 tttccgtgtt tcgttgatca gtttattttt tatttttgtc cgagtagatg ccaatacaaa
    13141 atctggatgg ggaaaaagac ggatcaatgt caatgcgtaa ggggtcagcc tgtttgaaaa
    13201 gaactgttca agctctggaa aagtcaattg aaggcagttg tgtaaatcca tgcgcagacg
    13261 agtgattttc ttctcaattt cttgataaaa acgggctaaa tcgcgcatct ctaagtaaaa
    13321 attgctttgt acttcttttg gctgacgttg atttttaaga tgagtctgtg cgagccggtg
    13381 ggcatccgat ttatctgttt tccaactgcg taaggtgctt tcttccatct gttttttcgc
    13441 ctctaacggg tttaacaaac agtaagaaaa gtgatgatct tgaaagaact tttcgactgg
    13501 tctggaataa atccccgttg cttcaaaaac aatcaatgga cgctcaggca agtgctggat
    13561 ctcttcgagt aatgcatgaa atcctctttg agtgtgttca atctcgtctt caaataagca
    13621 ggtgtcgtct ttgtatagga cactgtagct gtgtcccatt gaaacatcta atgcaagaat
    13681 gtaaggcatt tgttttcctc ctttttaagt tttgaaagaa ctttactgct tcatttcgat
    13741 tccaatttcc tatacacggg ctcatggccc caacatacgt aaacctgatt ctaaaatgac
    13801 agtaaagaag ttcggttttt gatagggact cgtagtcctc aaggctttgt tcgaactttc
    13861 tttcactctt cactataatc taaaaataga aaatagagga gaacatcatt cgtcaatgat
    13921 gttcccctct aatcttagta tgttttttat agtaattcct tcagatactt tcttgcctca
    13981 tcattgaaac gcagtgattc ttctaacttc tgataatatt ccaacgcttg tttctgagct
    14041 tcagtaaaac gataatgttt tcctttccat ttgtcgattg tctcttgcac atttccagat
    14101 tggatatctt tcactactag ttgccatttt tcttttgaga ctgtacggtc tttcttatca
    14161 gcagattgta tagcttctcg ttctttacgc aatactttta aaaaaccatt tctgactatc
    14221 cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc gacaatattg cttccaaata
    14281 aatagataga tttttggttg gatgaatcgt tggtttttcg caaaatattc catctgagca
    14341 gtgacttctg gtgttcgttg acgttttgtc tttctcaaca cggaaataaa tgtttcaaaa
    14401 taagcattag gatgtactgg cacttcttca tatacgatgg attgccaatc tttacgtaaa
    14461 aatgcgcgat agttgatcaa gaattcttct acatccttta ctaattcttc tgcaaagtcg
    14521 tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc cattgatgtg ttcacttatt
    14581 ttcttgattt cattttccaa tgcttgataa tccgctatgt aaatcggcat ttctacttgg
    14641 cgaaattctg cgagccgctg agctaatttt atatccggta aatatggctg tttcatccga
    14701 cgattttggt acaagacaaa acagtatttt tgccatagtc cttcaggaaa atcgatattc
    14761 ttctgcttaa gccataataa ttcgtctaag gccagatcaa cttttgtcat tccctgatgg
    14821 atcgaagctg ccactgtcgg agagacatgc aggtgatcgg caaagtggtt gatgcctagt
    14881 tttaggatct ttccggtttt tggcgattta acgatgtatt gcacttttaa ttctctacca
    14941 cactcacaat acaatttttg ctggcttcgt tttgttcgaa aaaacggatt gatcttgaag
    15001 tcagaaaaca cccaatctgt tgcagctaaa taatgttctt ccataaaacg agagctgatt
    15061 agatatcggc ggtgcttatc gactagttcc cgctgttttt tcgttaattt tgcatagatc
    15121 tcccaacgtt gactgcttgt gtattgttta gaaattttga tgggatagct tttgctgatt
    15181 tcttttggaa accattcttc aaagacggat tgccattctt ccacggctgt ttctttcctt
    15241 tctaattaca aaaagctggc tactaacata acgttagcaa ccagccagtg tttcttgcta
    15301 aattagtgag ttgttccggc atttccggaa atatcttctt tattttctac tgctgcttca
    15361 actgcataag tcaaagaaga agcaatgtct tctaaagaca tagaaggcgt acccactgga
    15421 cgatcgatca cttgatcggg tgagaatggc acgtggatga acccacctcg aattgccggg
    15481 aattgtgttt cgatcaaatg aagcaaacga tacatgatat cattgcagac aaatgttcct
    15541 gctgtataag agatgtacgc tggtagacca tgatcttgta cattttttgt cattgctttg
    15601 attggcaatg aggtaaagta agcggctgga ccgttttctt ctaaagccgt atcgaatggc
    15661 tgatttcctt cattatcagg gattcttgct tctgctagat tgattgcgac acgttcgaac
    15721 gagactgctg accgcccacc tgcttgtccg acacagatca cgacatctgg ttggtgctgt
    15781 tggatggctt ctttgactac ttgactgctt ttttcgaata cggtcggtac ttccactttg
    15841 atgatttcag caccgctgat ttcatctggc atttttttta ctgcttcata ggctggattc
    15901 actttgtccc caccaaatgg atcaaaacct gttactagta ctttcatctg ttgttcctcc
    15961 gtccgattga tttaacatat ggttatcttt tatcatacca ttttccttga acagcgtaaa
    16021 taaaggaaac aatagtttaa ataacataac agatcaaact taatatattc ttataaaaaa
    16081 atagtgttcc actacaatgt tagaagaggt tgcgacaatt attctgtcac aacccctctc
    16141 ttctattatt taaatagaat catataagca atttggaaaa ctagcataaa taacgcaata
    16201 aacaactggt ttttgatcac tccgtatttg tctttcatct ccagcatcgc tacaggcacg
    16261 atattgaagt tagcagccat cggtgtcagc agggtcccac aaaaaccgca agtcaatgca
    16321 accattccta ttaacgctgg attcgctcca tgactaaata cgaatggtgc accgatccca
    16381 accgtcatca cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg
    16441 ccaagagcgt aaatgatgat gccgatgttc acatttcctt ctggaacgat cgtacctact
    16501 gcattggaaa tcactgtccc aactccagcg cttgtaaata ccgcacctag agaagctaaa
    16561 agcattggca acatgcttaa cggccctact gtgcccagca tatctgctgc atcatctaaa
    16621 aatgtacttg gtcgattatc tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca
    16681 ccgacgccca cgccgactaa agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag
    16741 acaccgatac tcaaagctgg aataaaaatt ttcattccta gtttgtctga cattttttcc
    16801 atatattctt ttgatggaag ccggctttct cctttgctga ctttcttagc aattgctggt
    16861 atggtcatcg caaagatcaa gccaccgttc acgattgccg gaagccatct tcctcctgca
    16921 ataactaccc ctaagaccgt ccagaaaaag gctgacccat aacgatgagg attggtttta
    16981 tctgttaaat ttcttactcc agtgtaaatc aatacgagtc ccatcacgat ataaaggatc
    17041 tccagtaatt tatcgcctaa taaaatatca ggatttgtaa aaaagtccat cttattgccc
    17101 ctttcctgga taatatttct tcgctaattt cttttcttta agatagaagt agacacttgc
    17161 cacaactaaa gccgtgatag ccactggaac ttccactaaa gcaagctgtc ccaatgttac
    17221 gtcatatcct agatctttca gtgttgattg gactagcaac gcccctgctc ctccgatgaa
    17281 tagtacttgg ccaaagaacc agcagacatt ttccatagct gaagccatac cttttagttc
    17341 ttcttcatgc ttaggatgga cttctaaccc ttttgattcg atggtcccca atgccatcgg
    17401 taaaatgatt ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa
    17461 gatcattcga aagatcccat aaatatctac aatcatacca gcagatactt tcttgaatcg
    17521 cttgatcaag gaagccgctg attctttcag accatttctt tcaagcgttc ctgttgccaa
    17581 cataatgatg ataaaaatcg ccatcccgcg attgtttaca aaacttgtac ctaaaagttc
    17641 taacattcct tgaatgccaa gaccgccagt caaagctgta acaactaaag caaccattac
    17701 gatcaatatc gagtctaatt taaatgcaaa cccgagaata acaatcaaga cgcctaaaag
    17761 gctaagataa ttttccatat attttcctcc ataaaatgac aatatttttt taattataaa
    17821 gcaaaacaca taaaaaatat agttgttttc tcacgaaata gacactattt ttaaatattt
    17881 ttcgttattt ttttatcaca aaaattatct tacttattgt aaggtaaagc tttttttaat
    17941 ttattgcccg ataatatata gaaataaggt ctagttactg ttttttttaa atgattaata
    18001 aataacaact tctttctatg aaattgcaaa gcattcatct tcttttccgc taacgatata
    18061 tccgtcagtg cttggtacac tttactaaaa agtatgagat tgccgatatc tggataggtg
    18121 tgctatacta cagaaaaaga tatacaatct gccaccgggt aggataaaga ggatgatcat
    18181 tccattaggt ctttgaaggg atgattttcc tctttttgtg tattttttta tagttaagga
    18241 ggaatgtaag tatggcatgg atggaattaa tcatagcagg aattttggaa gtattctggt
    18301 caacgatgat gaaatggagt gatggttttt ctaaaatcaa ttattctttt tacacagtga
    18361 ttggcatgat tgctagtttt tactttttgt caaaggcaat caaaagtttg ccgatgagtc
    18421 tctcttatcc tatctggaca gggattggcg cagttggttc ggtcataatt ggtgtggtct
    18481 tgtttcatga taagctgaat ctttccactt ggttctttgt ggggatgcta ctcatcagta
    18541 tcattggcat caagattact agcggccact agacacttgg aataagtact gatcagaaaa
    18601 aataagcctt tttatttaat attcaatttt tttatgaaaa aataagaaat aagtccaatg
    18661 acctagcgac ttctccactc atcaggtgta tgcttgtact atctatagaa aaggagtgaa
    18721 tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac aaacaagccc
    18781 tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt tggattagta ggtcctaatg
    18841 gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac tcagatggaa
    18901 caatctgtat tggtgaaaat gagatttctc cctctagtca taaagggttg gaacaagtag
    18961 gagcattgat cgaatacccg ggtatctatc cattcttaac aggctatgat catctgaagt
    19021 tattctctga aaccaacgat gtatctgcta tcgacactat cgtgaatcag ctcaagatgg
    19081 aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg aatgaaacaa aaattgggaa
    19141 ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg atgaacggtt
    19201 tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct actcagggca
    19261 cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc gatgagctga
    19321 tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat ggctgaattg aaccaaacag
    19381 cgcaagattt catcgttatc tctacaacaa atgatctagc tgctcgaacg atcttgacag
    19441 aagctggata tactttggaa gatcctgata aaatcaagct tttaaaagaa aacgaagaat
    19501 tattaggaac cgtgatccag ctattgtctg cacaaaatat ccatgtgaca gacgtccagc
    19561 atctgacgaa tgatttggaa tcttctctat tggaaattct ggattcagta aagggggaag
    19621 aagaaaaatg acattgcttg taaaacaaga attatttaaa ttgatcaaga aaaagtcaac
    19681 tgctgtttta tctgttctat tagttgtttt gctcatagga acagcacttc tagccaaaaa
    19741 atacacgacg atcattgatc cagttgaaat gactgctcag ctttttagtg ccacatcttg
    19801 gattgtcttt atcatgattg cagctgcaag cacgatcatt tcgatggaag cacaatatgg
    19861 tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag taagcaaatg
    19921 gatcacatta gttatttatt ctgtttattt atacctttta gctattattg tcactgtgtt
    19981 gatgaaactg attcttttcc cttcgatttc cttcacggag aaggtttcta ctggtcaaac
    20041 actgatccaa agcttgttca cttatacttt gggtagttat atcggtttat ggctgatttt
    20101 aagtttagtg ttgatgattg cttgctttat caatagttcc ggcgcatcca tctcagcagg
    20161 aatcgtcttt tattttgctt cgtcaatcat atctggtatc cttattgcac tcatccaaaa
    20221 atgggagtgg atcaaatgga atccgatcag tatgttgaat ctgcaaaacc aaattggaaa
    20281 cgaagagatc atgaagcaat tgactcatct ttctactaat caaatgctat tcggtaattt
    20341 agcttacatc gttttgttct tagcattagg atatctcgta tttaagcgaa aaaatgtatg
    20401 atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc cttattgccg attggctgtc
    20461 tcttaaacac cgtttattct gaaaaaggag ctgtgacaac atttgtgtca cagctccttt
    20521 cctagtatgt ttttgaataa acgacaggct cacaacctct ctttatactt cttttatact
    20581 tctatacgtg aagcatccca gtggcaaata aaaccattgc aatcactccg accatcgtca
    20641 cacctataag taaaatctta tctaagtgga tatgttgtac aggctcgttt tgctccttac
    20701 gtgctttgag gtaaaaataa atgcctggca agaaaagaac aagaacaagc attacttcat
    20761 taaaggctac gactagcata gcccatactt gaaaggcact tgctaaaagc cctactaaaa
    20821 attggaccca ttctttccgc tgataagaaa gtttcatttg gtacaatcct aagaaaagcc
    20881 acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa gttataagca ctgtcagtga
    20941 ataataatga aagtaaaaag acattcgtac ataacgtcgt caatacgata gcataagttg
    21001 gtgccccatt tttgttgagt tttccccatg ctttaggcaa aagtccgtct tcagccatgt
    21061 ttcccattgt ttcaatcggt aagatcgtcc atgagagcca aacaccaact aaagaaatga
    21121 tcaaaccgcc gttgatcacg actgctcccc atttaccaac catcatttca aacacatagc
    21181 ccatagcagg ctgcgccttg atcgtttgca gctgattcat cgtcattgtc ccatatggaa
    21241 gcatggaaac caagacgtaa ataataatca agccgaatat ccctagaatc gttgctttcc
    21301 caacatctga ttgttttttt gctcgtgaac ctacgacaga ggctccttca ataccaacaa
    21361 atacccaaag catcaccatg atgcaactca atacttgact ggaaaacggg atcggctgtc
    21421 cattcgcatt caatataatg tcgctagaaa agttatcaat gaattgttgg atcgaaaacg
    21481 tgctgatagc tactacaata aataaaaata atggcactaa tttacagaat gtgacgatag
    21541 cattgacgaa actagcattt tcgactcccc gattcactag ccatgcatat ccccataaaa
    21601 agactgtact catcacgatc gatgccagat tttgcccacc ttcaaatact gggaagaaat
    21661 atcctaatgc gctcatcaac aatgttgcaa aagctacgtt gcctaaccag catgtgagcc
    21721 agtaacccca accgctgata aaaccagcga aacggccaaa accttcttct gcgtaactaa
    21781 agatcccgcc ttttaaatct ggacgttttt tattgatatt attgatcgac aaggagagca
    21841 tcaacattgc tgttcctacg agtacccaac tgaaaatagc tgggccgcca gccgatccgg
    21901 agatgtccgt cataatacca aatacgccac ctccaatcgc tgaaccaatt actaaggcag
    21961 caagtggaat taaaccaatt ccttttttct gttgctctgt atccatgtaa gcatcctcca
    22021 aaataaaacc gcttttatca aaaagtataa caaacctttt ttatttttac ttaattaata
    22081 aaaactttaa tagaaatata attctaccgg cttttttctc attatacaag caaagcgcct
    22141 agtccctttc caaattacac caccaaaaaa cttaaaaaag atgctatact aaaaggaaag
    22201 aattggagga tgatccatga acattttata tatatcgatt tcaggaaata cgcgtgcctt
    22261 tgccaaacat ctagcagaat atgctgaaaa aatgcatgca gaagatcctg tcaatcctga
    22321 agttacttta aaagaaatcc atgaaaatag tgattttacg aaagaaacag aaccattttt
    22381 tactttcgtt cctacttatt tagacggcgg aaatggttta gataacggtg atacagaaat
    22441 cttgaccgaa acgatgcgtg aatatctgga acatgaagac aatcataaac tttgtcttgg
    22501 tgtagtcggt agcggaaata aaaacttcaa taatcagtat tgtctaacag ccaaacaata
    22561 tgcacaaaca tttggctttc ctttcttggc agattatgaa ttacggggaa caccatctga
    22621 tgtgacacga atttatcaga ttttagcaga aaacaataaa taataaaaaa gaagaagctg
    22681 aaaaatagat ttcagtttct tctttcgttt ctattgtttc tatcatatta agctttgaca
    22741 tccatcacag gtgcttttgc ttttgcttta cctgtttttt ccaagacaaa gttttttaca
    22801 cgatccatat tggttaccaa gacgatcatg gatgtttctt tgcctgctgc tttgatcgct
    22861 tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgcccttc tgttactttc
    22921 acatcaaatg gcgtaccgcc taagtcaact gtgttgattc ccatatgaag caagatttct
    22981 aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg ggaagacaga gctgactacc
    23041 ccagtaactg gagaatagat atgtccgttt tctggttcta caaagtagcc ttcacccatc
    23101 atcttttgtg caaaaactgg atcacttgct ttatctaaag aaacaactgt tccgtcagca
    23161 actgatgaga ggatagtcac actttcagct gaagtttgag gtactacttt ttcaggcact
    23221 tcatttactg tggctgtttc agtcgctaat tcttcttttt ctaaacggtc tttaggaata
    23281 ccgaagaaga atgtagcaac gaagccacca gcataagctg ccaataatcc aaggatgtag
    23341 cctaaccatt taccgtcaga gatcaatggg atcaaagcgg ctcctgaagg tccaatagcg
    23401 atagccccga catttccgat cattccgatc actgccccgc cgatacctcc accgatacaa
    23461 gcagtaataa atgggcgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt
    23521 cctaggatcc cgactggtaa agctcctttg atcatttctg ttaattcttt atcttttttg
    23581 cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg caccagccat cgcaagaatt
    23641 ggaagcaata gcgtacgacc agtttcatca atcatttgca catggattgg agtcaaaatt
    23701 tgatgcagtc cgaacataac catcggcaag aataatgtgc ctagaacgaa gccggcaaat
    23761 gctccaccaa cgccaagtac ccagttgatc ccaccgacca tgccatcaga aatgaaacct
    23821 gctaaaggca tgatgaagaa aatttcaatc agaccgatgg caatcaatga aagagttggt
    23881 gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac
    23941 aatagccaaa cagcgaagat gacacctagg atcccacctt gtccagctgc aagagcttca
    24001 ccagtaaata agtttttcag tggtgcttcc ggattcatcc ctgtcagcat cacaacggcc
    24061 ccgataactc caccaagagt tggtgttgca ccaaaaactt gtgcagcatt gatacctgta
    24121 tagataacta ggtagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt
    24181 tgccatgttg ctgcatcaag tgttccggct gtgactaagt ttgctaaaac tgcggcaatc
    24241 cctgccacga tcccagtacc gacaaatgca ggaatcattg gcacaaagat attagaaatg
    24301 tcttttaata ccgatttgat tttagatggt ttttgtttcg atttttgtgc cgctttcatt
    24361 tcttgtgccc gttcgtttac acggtctttt ccagatgctg atgaatttgc tgtagctgta
    24421 cttacagaag aaagtttttc tcctaatttc acgcctgcca aatcaaccat ttgctgtgct
    24481 actttgttca ctttcccagg tccgatgatg acttgaagct gttcatcttc tactacacca
    24541 agcactcctg gaatcgcttt taattcagag acatttacaa gatcatcatt gatgatcttc
    24601 atacgcacac gagtcatgca gtggacaatg ctttctacat tgcccatgcc gcctgcccct
    24661 ttataaattt gttttgccag ttcttccaaa gtcagttcgt tttttgccat ggtcttttcc
    24721 ccctcataag gtccctttta caaatccgtt tgcttcagct aattttgctt gagcttcctc
    24781 tttatcacta tttgtcaaaa tcatgacgat agccagctta acattttgat ctgcttcttc
    24841 aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct tgcatgatga ttcgttttga
    24901 gcgttcgatt agtttttcat ttgttggttt gacatcgacc atcaagttat tgaatacttt
    24961 acctatacca atcatactga tagtagaaat catattcaag ataagttttt gtgctgttcc
    25021 tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct gcatcgactt cgattgggaa
    25081 atccgcgtgt ttactgattt cggcttgttt attgcaagaa atcgttcctg tgacagcacc
    25141 gatcgatttt gcgtaatcta atccaccgat cacatatggt gtacgaccgc tagctgcgat
    25201 accaataact atatcatttt ttgtcaaatt cagcttgatt agatcttctt gccctagctc
    25261 ataagaatct tcggcccctt cgacagctac agtcattgct tcttctccac cagcaattag
    25321 acctatgacc atttccggtt ctacaccgaa tgtcggtacg cattcagcag catctaacac
    25381 acctagtctt ccacttgttc cggctcccat ataaatcaga cgtccacctt ttttaaagga
    25441 ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt tctttttcaa ccgcagcggc
    25501 cactttctga tcttctttgt tcattagttg aacggcttga gaaatactca tctcatctaa
    25561 tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat
    25621 ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc
    25681 aatggtagct cttctttgat aactttagct actacattca ctttttcatc cgctggcaaa
    25741 tcatatttgg ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta
    25801 atagaccctt tcatacgcgt agctgtattt cttgctggga taggcggaat ctctcgaaaa
    25861 cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt gccgattcgt atgcttccct
    25921 aatatatatt cgtaaaattc ttcatctaca ggttccgtat ggagcaagac tgtctgttct
    25981 ttttggaaag aagcaaattg ctcctgaacg ttttctgaca gacctgcatc tccgatgtaa
    26041 acaatatccg tgttacttgc taatagatct agagcgcctg caagaggatg gatccctcga
    26101 tgttcttcca aagttggcaa tccttgatat agtgggcctc tcaactctgt atctcctggt
    26161 acaaaacctt ggacaaggag accttgaatt ttgagccatt gatttttttt ctgataccat
    26221 tctttgtcca gccctgtttc gggacgtgga taataattat gccaagcttc aatctgtgag
    26281 aaatcagctt ctgcttcttt caattcatcg atatctttag gcgtgatcgt gctagcattc
    26341 aaactgattt tcaattgatg tgaccagtcg gcaatctgct gattggaaat atgataatcc
    26401 attcttaaac ctgttacacc aattgctttc aaaggcgtta gatcttctaa tgaaaaaccc
    26461 gctcgttcca atgcctctcc tgaaatatcg accatcaatt ctagtttatt ctttttcgct
    26521 tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa catcatcttc tggtatatgc
    26581 atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc
    26641 tcttctgaca aagaatcgtt cataaatatt gaaaagccaa acatattatc cctctttttc
    26701 tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaatact
    26761 tttattaaaa taattaaatt tcatttctaa aggttgagtt tatattatca aaagaattgt
    26821 cataacctaa aaaaggcact gctaagcaaa agcagtgcct tgaaatattt tatttaaata
    26881 acacaaaaac ataattactc ataagaaacc aattctgtat tatctaccat gaatgtaaag
    26941 aacccctcat taggcatcgg atgaaaataa gcattgatca ctcttatcaa ggcattatgg
    27001 caaactaaca gatagacatt ttcttcgtct tcgatgcatt ctttcaataa aggaacgatc
    27061 cgtgcataca catctaagac tgactcccca tttgggaatc gtacagcgaa cgctaaacgt
    27121 gctttttgaa agttctcatc tttgcttggc agtccatcat aatccccaaa atccatttct
    27181 accaatcgtt catcaacggt cagcggcaag ctgagacgat cagcaactgc ttgtgctgta
    27241 tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact
    27301 accttttctg ctagtttctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgcc
    27361 cgtccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc
    27421 atgtgattct ctccttcgtt ggcaaagttc cccttaacca taccttgttg ttcttgagat
    27481 tacaagggtt agattctctc aagagaaata atctcagtag atactttatc caggaaggtt
    27541 tgatcatgtt ccactagtaa catggcaggt ttcacgttca agatcagttg ttctaattgt
    27601 tcttgattga agacatccaa ataattcaat ggttcatccc atgtatatag ttcagctggc
    27661 tgtgataaag atttagccaa ttccactttt ttccgttggc ccatactcat ctgctcgatc
    27721 ttgttatgaa aaacatctct ttccatccca agctttcgga ggttgttcaa aaatgcttgg
    27781 tagtctactt ggtttttctc tgcaaattcc gccaacgttc ctcgattgtc ttcataattt
    27841 tggcttgcat aactaatgct cagatgtttt ggctggtatt tttctcctat aaccttgccg
    27901 ttgaatgccc ccagaagata atggataatg gatgactttc ctgcaccgtt aggacctgaa
    27961 atcgccaccc gctgatgagg ctcgattgta aaatgaattg gctcgaataa cagattttca
    28021 taccctaatt gaagatcttc tacgcttaaa agtcgcttat ggtgagacgc ttggctattc
    28081 atcgtcaaag aatcgatata ttcgatatct tttaatagtt tttctttctc gctgatctgg
    28141 gtctccatcc gattcacgat tgctttggat cgtttcatcg tccgtgcagc atcagcaccc
    28201 actgctcctt tattcactcg tctagattca gtatcgatga atccgacttg tttctttgtt
    28261 ttatctcctt ctcgggaacg agaccattcg gctttttcag ctgctgtttt ctttagccta
    28321 ctgacttctt ttttcaattt ttcattttga gccatttcaa attcatcacg aagttttttc
    28381 tgttcttcat agatagagaa attcccttga taaagttcca gttgactttt ttcgattgct
    28441 aaaacatggt ccactacttc atcgataaat ccccggtcat ggctgaccac gatgaagcct
    28501 tgttttttct ttttcaaata agccgctact tgttttctac cagagatatc caaatgattc
    28561 gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataaaagtgc cagtaggact
    28621 tttgtcttct ctccccccga tagtgtgctg aattctctcc ataagatttc aggatctgtt
    28681 tgcatcaatt ggagctctct ttcgatttcc catatctcaa aatccgtaat atcatttaac
    28741 acgtaatagg ttaaacgttc tttatctttt gtctgttgcg ggaaataggc aaattcttgc
    28801 tgatggatta cttgcccttg ataaggtagt ttgttttgta gaatattcag taaagttgtc
    28861 tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa atttagattt
    28921 gcttgttcaa ataataatgt gccttggctg tcgtagccga atgtcagatt ttttatttcg
    28981 attttagaca ttttccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta
    29041 aagaggatgc aagggttgga tacacacaaa aagacccata catcgaaaaa accaagccaa
    29101 tcccgaaaat ctgcacactg cttccttgtc tccacaaggt acagtctgaa tgcagctttc
    29161 atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac
    29221 gatagcataa agaaaatgat tttgtcaact acttcgggag gatggtgaaa aatctgctaa
    29281 gtgtctgtcc taagattttt gttttttcta atgtttgcct gtgttccatt gcttcttcta
    29341 aacctattgg acccaaatga atacttaaga caattgggag aaaatcagaa agtccttcta
    29401 gctcttggtc tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca
    29461 ctgctacacc gtatggaact tttccgtgga tcgtttgctg atcgatccgc ccttctcctg
    29521 taatgatgac atctgcttgt tcgatctttt tttccaatcc agtgatttgg ctgactaaat
    29581 cgaaaccaga aaccatcttc gctcccaaca aaaaaagggc tccaccaata ccgcctgctg
    29641 ctccacttcc tggaatatca tctaaatcca cctcccagaa atcttttatt tgctgagcaa
    29701 tctttcctgc ttgtttatct aagaaagcta gcgtcgtatc tattcctcct ttttgtcttc
    29761 caaattgcca agcagctcct tgttgacctg tatatggatt ggtaacatct gcagcgataa
    29821 tcaattttac tttagaaaca agaacgtttc ttcgatcaaa atgaagtttt ttagtagata
    29881 ataaaggatt tccttctgtg tacccttcta aagaagcacc caaggattgg agtaatccta
    29941 gaccaccatc gctacacccg cttcctccta aagtgacaat aatttgttct acttcttgaa
    30001 gcagtgcatc tttaataagt tctcctaagg cataactgga agcaagtcgg atagtttgat
    30061 ccgttggttt atcaagtaga tctaacccca atgcttgtgc agattcgatg actgcggtgt
    30121 tttttccttg ccaagttgtc aataaatagc tgatttttcg ctgacggaat aagagatcca
    30181 tcccatcaat ggtacgccat gttcccctga gcgcgtaata gattgcttct gcagtatttt
    30241 ctccgccatc tgcaatcggc acattgattt ttcttccttt acgatcttcc cagccattta
    30301 gaactgcctg atttagttca aatgaagaag cacttccttt aaatgaatca attgccgcca
    30361 aaatatccat ctgtatccct cactttcttc ttaagtaaga cacttttttt catttttttc
    30421 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
    30481 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
    30541 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
    30601 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
    30661 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
    30721 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
    30781 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
    30841 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
    30901 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
    30961 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
    31021 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
    31081 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
    31141 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
    31201 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
    31261 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
    31321 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
    31381 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
    31441 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
    31501 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
    31561 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
    31621 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
    31681 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
    31741 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
    31801 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
    31861 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
    31921 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
    31981 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
    32041 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
    32101 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
    32161 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
    32221 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
    32281 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
    32341 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
    32401 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
    32461 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
    32521 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
    32581 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
    32641 ttctgaatag ttgtaattgt ct
//
LOCUS       DASJTM010000025        32497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJTM010000025 DASJTM010000000
VERSION     DASJTM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.025"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="U4B29_001086"
     CDS             complement(<1..563)
                     /locus_tag="U4B29_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN2106049.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="U4B29_001087"
     CDS             complement(750..1400)
                     /locus_tag="U4B29_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106050.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U4B29_001088"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U4B29_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN2106051.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="U4B29_001089"
     CDS             complement(2912..3910)
                     /locus_tag="U4B29_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN2106052.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U4B29_001090"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U4B29_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN2106053.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="U4B29_001091"
     CDS             complement(4926..5657)
                     /locus_tag="U4B29_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN2106054.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="U4B29_001092"
     CDS             complement(5661..6926)
                     /locus_tag="U4B29_001092"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN2106055.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U4B29_001093"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U4B29_001093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN2106056.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U4B29_001094"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U4B29_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN2106057.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="U4B29_001095"
     CDS             12268..13770
                     /locus_tag="U4B29_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2106058.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="U4B29_001096"
     CDS             13772..14509
                     /locus_tag="U4B29_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2106059.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="U4B29_001097"
     CDS             complement(14680..15873)
                     /locus_tag="U4B29_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2106060.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="U4B29_001098"
     CDS             complement(15952..16158)
                     /locus_tag="U4B29_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2106061.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="U4B29_001099"
     CDS             complement(16151..17293)
                     /locus_tag="U4B29_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2106062.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="U4B29_001100"
     CDS             17805..18440
                     /locus_tag="U4B29_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2106063.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="U4B29_001101"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="U4B29_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="U4B29_001102"
     CDS             18916..19032
                     /locus_tag="U4B29_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN2106064.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="U4B29_001103"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="U4B29_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN2106065.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="U4B29_001104"
     CDS             21086..21253
                     /locus_tag="U4B29_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN2106066.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="U4B29_001105"
     CDS             complement(21313..21666)
                     /locus_tag="U4B29_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106067.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="U4B29_001106"
     CDS             22170..22589
                     /locus_tag="U4B29_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2106068.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="U4B29_001107"
     CDS             22615..22821
                     /locus_tag="U4B29_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106069.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="U4B29_001108"
     CDS             complement(23049..23222)
                     /locus_tag="U4B29_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106070.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="U4B29_001109"
     CDS             23198..23863
                     /locus_tag="U4B29_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106071.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U4B29_001110"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U4B29_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN2106072.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U4B29_001111"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U4B29_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN2106073.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U4B29_001112"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U4B29_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN2106074.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U4B29_001113"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U4B29_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN2106075.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="U4B29_001114"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="U4B29_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="U4B29_001115"
     CDS             complement(30257..30484)
                     /locus_tag="U4B29_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN2106076.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="U4B29_001116"
     CDS             30968..31558
                     /locus_tag="U4B29_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN2106077.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31748..32461
                     /locus_tag="U4B29_001117"
     CDS             31748..32461
                     /locus_tag="U4B29_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106078.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DASJTM010000026        31620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJTM010000026 DASJTM010000000
VERSION     DASJTM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.026"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..853)
                     /locus_tag="U4B29_001118"
     CDS             complement(5..853)
                     /locus_tag="U4B29_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2106079.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1077..3017)
                     /gene="thrS"
                     /locus_tag="U4B29_001119"
     CDS             complement(1077..3017)
                     /gene="thrS"
                     /locus_tag="U4B29_001119"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN2106080.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3354..3533)
                     /locus_tag="U4B29_001120"
     CDS             complement(3354..3533)
                     /locus_tag="U4B29_001120"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN2106081.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3580..5403)
                     /gene="cadA"
                     /locus_tag="U4B29_001121"
     CDS             complement(3580..5403)
                     /gene="cadA"
                     /locus_tag="U4B29_001121"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2106082.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5681..7960
                     /locus_tag="U4B29_001122"
     CDS             5681..7960
                     /locus_tag="U4B29_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2106083.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8053..8700)
                     /locus_tag="U4B29_001123"
     CDS             complement(8053..8700)
                     /locus_tag="U4B29_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106084.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8879..9598)
                     /locus_tag="U4B29_001124"
     CDS             complement(8879..9598)
                     /locus_tag="U4B29_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2106085.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9729..10880)
                     /locus_tag="U4B29_001125"
     CDS             complement(9729..10880)
                     /locus_tag="U4B29_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN2106086.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11097..11837)
                     /locus_tag="U4B29_001126"
     CDS             complement(11097..11837)
                     /locus_tag="U4B29_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2106087.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11841..12164)
                     /gene="rsfS"
                     /locus_tag="U4B29_001127"
     CDS             complement(11841..12164)
                     /gene="rsfS"
                     /locus_tag="U4B29_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN2106088.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12221..12814)
                     /gene="yqeK"
                     /locus_tag="U4B29_001128"
     CDS             complement(12221..12814)
                     /gene="yqeK"
                     /locus_tag="U4B29_001128"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN2106089.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12798..13448)
                     /locus_tag="U4B29_001129"
     CDS             complement(12798..13448)
                     /locus_tag="U4B29_001129"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN2106090.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13458..13769)
                     /gene="yhbY"
                     /locus_tag="U4B29_001130"
     CDS             complement(13458..13769)
                     /gene="yhbY"
                     /locus_tag="U4B29_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN2106091.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13785..14894)
                     /gene="yqeH"
                     /locus_tag="U4B29_001131"
     CDS             complement(13785..14894)
                     /gene="yqeH"
                     /locus_tag="U4B29_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN2106092.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14891..15418)
                     /locus_tag="U4B29_001132"
     CDS             complement(14891..15418)
                     /locus_tag="U4B29_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN2106093.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15598..16383)
                     /locus_tag="U4B29_001133"
     CDS             complement(15598..16383)
                     /locus_tag="U4B29_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN2106094.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16376..17245)
                     /locus_tag="U4B29_001134"
     CDS             complement(16376..17245)
                     /locus_tag="U4B29_001134"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN2106095.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17246..18625)
                     /locus_tag="U4B29_001135"
     CDS             complement(17246..18625)
                     /locus_tag="U4B29_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN2106096.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18633..19052)
                     /gene="fabZ"
                     /locus_tag="U4B29_001136"
     CDS             complement(18633..19052)
                     /gene="fabZ"
                     /locus_tag="U4B29_001136"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2106097.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19077..19553)
                     /gene="accB"
                     /locus_tag="U4B29_001137"
     CDS             complement(19077..19553)
                     /gene="accB"
                     /locus_tag="U4B29_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN2106098.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19556..20791)
                     /gene="fabF"
                     /locus_tag="U4B29_001138"
     CDS             complement(19556..20791)
                     /gene="fabF"
                     /locus_tag="U4B29_001138"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN2106099.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20810..21547)
                     /gene="fabG"
                     /locus_tag="U4B29_001139"
     CDS             complement(20810..21547)
                     /gene="fabG"
                     /locus_tag="U4B29_001139"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN2106100.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21559..22476)
                     /gene="fabD"
                     /locus_tag="U4B29_001140"
     CDS             complement(21559..22476)
                     /gene="fabD"
                     /locus_tag="U4B29_001140"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2106101.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22583..22807)
                     /locus_tag="U4B29_001141"
     CDS             complement(22583..22807)
                     /locus_tag="U4B29_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2106102.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22860..23822)
                     /locus_tag="U4B29_001142"
     CDS             complement(22860..23822)
                     /locus_tag="U4B29_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN2106103.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23832..24272)
                     /locus_tag="U4B29_001143"
     CDS             complement(23832..24272)
                     /locus_tag="U4B29_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2106104.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24578..25543
                     /locus_tag="U4B29_001144"
     CDS             24578..25543
                     /locus_tag="U4B29_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2106105.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25600..25830)
                     /locus_tag="U4B29_001145"
     CDS             complement(25600..25830)
                     /locus_tag="U4B29_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN2106106.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25916..26803)
                     /locus_tag="U4B29_001146"
     CDS             complement(25916..26803)
                     /locus_tag="U4B29_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN2106107.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26892..28115)
                     /locus_tag="U4B29_001147"
     CDS             complement(26892..28115)
                     /locus_tag="U4B29_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2106108.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28132..29178)
                     /locus_tag="U4B29_001148"
     CDS             complement(28132..29178)
                     /locus_tag="U4B29_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2106109.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29342..31069)
                     /gene="ptsP"
                     /locus_tag="U4B29_001149"
     CDS             complement(29342..31069)
                     /gene="ptsP"
                     /locus_tag="U4B29_001149"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN2106110.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31069..31335)
                     /locus_tag="U4B29_001150"
     CDS             complement(31069..31335)
                     /locus_tag="U4B29_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN2106111.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         8707 a         6943 c         5439 g        10531 t
ORIGIN      
        1 gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt ttcacttttt
       61 cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc gtagcaaatt
      121 gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt ctagatgttt
      181 gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga
      241 tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca cttctacctg
      301 agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc gggagcaaga
      361 cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt tgcagaatat
      421 cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct gccgattcca
      481 aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt tcttttatcg
      541 ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca tttgcttcta
      601 tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg attttgaagt
      661 ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc gcactcaacc
      721 cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata ttaccgataa
      781 tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt tcaatacgac
      841 taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt ataccatatc
      901 agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg cccaacagaa
      961 tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga ggaatacctg
     1021 ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt tacctattag
     1081 ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat attcaaatct
     1141 tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc ttccaattcc
     1201 ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg tatcttatag
     1261 cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg caaacgttct
     1321 ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat tgttgcttgt
     1381 ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa
     1441 cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt attttccccg
     1501 tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa ttgtatagtt
     1561 gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt tggtccgtaa
     1621 aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat agcggctttc
     1681 agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc tgtattgttt
     1741 ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc atatactgcc
     1801 accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg aacaaatgta
     1861 tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt
     1921 tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg ataagaatgg
     1981 atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag aacaagcatt
     2041 tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc ccagtgtcca
     2101 gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg gtaacctaga
     2161 ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc cccttttgga
     2221 agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc aagttctttt
     2281 cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata ttctttcaaa
     2341 gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt gtttgagttt
     2401 ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg gccagtggat
     2461 ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc agtaatcact
     2521 tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc aaaaatttct
     2581 aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt gacgattttc
     2641 atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg tccattatct
     2701 gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat atttggatag
     2761 aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa gccaagtgca
     2821 tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg acgagtcaaa
     2881 tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct ttttgaaatg
     2941 ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc accatctgga
     3001 aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa
     3061 gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg aaaagttcgt
     3121 ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc
     3181 cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg aaaagtttca
     3241 gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct catcaatcaa
     3301 ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta aacttatttt
     3361 ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat gacatggatg
     3421 tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt catcatattt
     3481 tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg cataatgctc
     3541 tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt ttgtcttggg
     3601 tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca aaatagagac
     3661 cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc ccgtacttgc
     3721 aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt tcgctattct
     3781 catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat ctttcaagat
     3841 gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg caataccgat
     3901 atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg caacaggtct
     3961 ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc tacgggcatg
     4021 tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc catccccggt
     4081 gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct ttgctgcttc
     4141 tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata
     4201 gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac gtccaatctt
     4261 tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt tcagttgact
     4321 aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta agatatggct
     4381 agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta ttccattgac
     4441 tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt tatcaaaagc
     4501 aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa cgatcccgtt
     4561 tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa tcagtggaca
     4621 aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga
     4681 aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat cagctaaacg
     4741 gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca tttgatactg
     4801 gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat ttaaagaccc
     4861 tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg tcaaagaaga
     4921 ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa gttcgccagg
     4981 cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct gcaattttcc
     5041 ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca gttctttacc
     5101 cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga tcaatatcat
     5161 caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa tcgctaatac
     5221 atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc
     5281 gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa
     5341 ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt tgaaatgtgt
     5401 cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt tccagctgcc
     5461 tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc cttaattgaa
     5521 aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa aatattcagc
     5581 aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa ctatgctata
     5641 cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga aacaactaga
     5701 acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata tgctggatac
     5761 aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag ctaatcacaa
     5821 gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc gccaacatga
     5881 gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc tgcaaacgtt
     5941 gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag accgtgaaac
     6001 agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa caatcgttat
     6061 tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc ccgcatttta
     6121 tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat ttaaaatcca
     6181 agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg actttttgct
     6241 agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta cgatacaaaa
     6301 agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg aaggaatcgc
     6361 cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt atcataatcg
     6421 caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt tttctgatta
     6481 catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga cattcaaaaa
     6541 ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac aagaaagtgg
     6601 ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga ccttagtcga
     6661 tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc gtgatatgaa
     6721 aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata aagaaacaaa
     6781 tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc tgaaaaaatt
     6841 aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg cggaagaaca
     6901 gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa aggagcgttc
     6961 attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg
     7021 gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc tacaaactgt
     7081 gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg aacatttgac
     7141 aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg tactattctt
     7201 tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct atatctttat
     7261 tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct tgttcccaaa
     7321 agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt
     7381 tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat taacgacaag
     7441 ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga aagaacatca
     7501 agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa caacagatga
     7561 tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag cccggtattg
     7621 gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg ctcaactgcc
     7681 agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta tgaagcgttc
     7741 cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag ttttcttgtg
     7801 ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt acaccatgtg
     7861 tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc ctttgttaaa
     7921 caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac aactgctatc
     7981 attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag taattcaatc
     8041 atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt ttaatttgac
     8101 aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt caaatacctt
     8161 gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc attcaatgaa
     8221 ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa tctctgatac
     8281 acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta tcttctcatt
     8341 tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata gtagaaagaa
     8401 agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag
     8461 cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca ccatacactc
     8521 taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc cttcaacgcc
     8581 gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag ctaactcttc
     8641 tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt ttgcttccat
     8701 attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact tatgctctca
     8761 tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt
     8821 tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt
     8881 agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct tccagtgcat
     8941 caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg ttttgaggga
     9001 tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat gcttcttgta
     9061 tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct tccattacat
     9121 cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg tcttctcctg
     9181 caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt
     9241 tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc acattgttcg
     9301 tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag cgcaattcag
     9361 agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata atatgttttg
     9421 gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt gcatgcggat
     9481 caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca taaatccggc
     9541 tattattagc atctttagca gttttctttg ctacgatatt tgcccattta cgtcccatat
     9601 tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt tccattatac
     9661 ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc tcatttttag
     9721 tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat attgcctccc
     9781 aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc tttttttccg
     9841 attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc agcatcggta
     9901 aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg ctttttgata
     9961 ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct ttttgttttt
    10021 acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt catgcgcgat
    10081 tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga agataacaac
    10141 cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt tgaaagatct
    10201 tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa cgcatggcga
    10261 atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata gcctgcactt
    10321 ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc ctttgcataa
    10381 cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg atacagactg
    10441 cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag ttcttgaaat
    10501 gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct gtaatcaaac
    10561 gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg caagattttt
    10621 actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg aagctccact
    10681 actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc gatgatagcc
    10741 ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc tgctcctgtt
    10801 atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt gtactctacg
    10861 ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa aagaaataga
    10921 gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac tattctcgca
    10981 aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata tcgttcggat
    11041 atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca aaaccgctag
    11101 tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa
    11161 tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa ataactttcc
    11221 aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc agacccttct
    11281 tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct tcctggataa
    11341 ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc tggaaaaacc
    11401 tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc ttctaacgct
    11461 tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata gcacagcgaa
    11521 tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag catattgcct
    11581 tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat catcaacatc
    11641 tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc aaccgctaat
    11701 gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc
    11761 gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta aatgaaagca
    11821 aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag cggagcatct
    11881 gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac atgtacgatc
    11941 acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat acgttttact
    12001 tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt gatttgacgt
    12061 tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt tcgaacgtct
    12121 aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat ttttaattgt
    12181 tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt gctacccagt
    12241 aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc aaatgggcca
    12301 atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca atggctcgtg
    12361 catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca taaatgattt
    12421 tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca tgaagaatct
    12481 ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga ccatgccaga
    12541 ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt accattttga
    12601 actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata ctagcttttt
    12661 caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact ccaagcacat
    12721 gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt tctctagaat
    12781 aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg aatataatca
    12841 attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt ttgacgtatt
    12901 ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata tggagtatcc
    12961 gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag ctcatcgatt
    13021 ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta ataatctgta
    13081 tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt cccaccacga
    13141 gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg
    13201 ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa
    13261 ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc catcactaaa
    13321 tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt tttcttctct
    13381 aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt agcttgcgta
    13441 ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg atacttttct
    13501 tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat ttcgcaatct
    13561 atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt ttgaagtaac
    13621 gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat cattgctgaa
    13681 ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa
    13741 aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat caatgctttg
    13801 cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg cgctgttaca
    13861 gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa
    13921 cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa taatccacct
    13981 acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat atttacatca
    14041 tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt
    14101 ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt gatttctttt
    14161 aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg atggcgatgg
    14221 ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc gattttatcc
    14281 aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc tgtatttttg
    14341 atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat atagacatcc
    14401 cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg agatttctta
    14461 gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg ttccttcatc
    14521 cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt gttcccaact
    14581 aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga accattgaag
    14641 tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc attcagtaag
    14701 cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg gcgtaatctg
    14761 aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc cgtttgcggc
    14821 gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc gatacattgt
    14881 aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt gctaatagat
    14941 aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta tctgttgcga
    15001 taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt atatcagtca
    15061 taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt ttctttgcta
    15121 tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt
    15181 caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca gggattcctg
    15241 catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga ttattccatg
    15301 caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc tttaattggg
    15361 caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt gaaaacatgc
    15421 tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc ccgtattata
    15481 ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa aagcatttct
    15541 ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa aaaacaacta
    15601 atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta atcgaacaag
    15661 ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat atcgtggcaa
    15721 ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact cgcgtagttc
    15781 tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt ctttccacaa
    15841 aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca gcatccagac
    15901 ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa tgatgatggc
    15961 gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag cctctccttc
    16021 gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca aggtaatcac
    16081 cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat agccttcagg
    16141 atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc
    16201 gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg cttcgtcatc
    16261 tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt caaaaaaatc
    16321 caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg ctgtcttatc
    16381 cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt ttctgtttca
    16441 attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat tcagcctttt
    16501 ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac
    16561 cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa ctggcagtta
    16621 ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca ttgctatgcc
    16681 gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct tcttgcatcc
    16741 gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt gctcgttcga
    16801 ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata aaattaggat
    16861 ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg aggactgctt
    16921 catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg ggaaatgcta
    16981 gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca tcaataatca
    17041 aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg catacttttt
    17101 cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt gcccacatgt
    17161 tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg atccgaatat
    17221 atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct ggtgtccaat
    17281 tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg actgcaggat
    17341 gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg ataaattcac
    17401 ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg acgattattt
    17461 ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact gcactgtcta
    17521 ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct ggagaaggag
    17581 caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt ccttggaata
    17641 ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc tgttttttta
    17701 ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt cgagtattca
    17761 tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt
    17821 cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct ctttttgttt
    17881 gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt tgtaaagaac
    17941 aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc tgaacttcta
    18001 tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca aatgccgctg
    18061 ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat tgaactttcc
    18121 gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa ccaattctgt
    18181 ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct ggaatgactg
    18241 ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt gcatcgatcg
    18301 ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag gcaaatcggc
    18361 tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc acgattgcag
    18421 cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg ccaatgcaga
    18481 ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta
    18541 cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt tcaccgcggt
    18601 tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt taattccgct
    18661 tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc tgaagctttc
    18721 actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt cttacggaat
    18781 tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc aagagaaagt
    18841 aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg cataacaggt
    18901 tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt cttagcaacg
    18961 atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg ataacgatgt
    19021 ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg ctttgcttat
    19081 ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt ttctacaagg
    19141 atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt catcgcttca
    19201 ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa agctggtttg
    19261 tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc gattccatca
    19321 aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg ttcaggtatg
    19381 acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt catgcctcga
    19441 cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc gaattccgtt
    19501 agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc caaaattatt
    19561 cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat gaattactca
    19621 ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag tcaatctcag
    19681 gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat gccagcaaag
    19741 ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc gatttcgtgc
    19801 tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct tttgcttctg
    19861 cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact tgttccggct
    19921 gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa ccatctggag
    19981 taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt tctccataga
    20041 ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg cctgctcctt
    20101 cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga ttaggatcag
    20161 ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca caaatcgttg
    20221 cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta cggaatgctt
    20281 ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa ataccttttg
    20341 cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt ggtacgaaca
    20401 ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt tcgattgttg
    20461 gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc attttttctt
    20521 tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag atcgaaaaca
    20581 tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa tctttgactt
    20641 cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt gcgatgccat
    20701 tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga
    20761 ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct tatccgtgca
    20821 tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt tgtgctaaga
    20881 aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga atttgctttt
    20941 ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg atgaatcctg
    21001 gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca gattttgtta
    21061 atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt ccaatcaacc
    21121 cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt tttttcaata
    21181 cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt ttgaaatctt
    21241 ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt actaacacat
    21301 ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc tcataatcag
    21361 agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct tctatctttt
    21421 cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt gcaaaagcta
    21481 aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt tttcctttta
    21541 catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct gcttattctc
    21601 aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta atacttttcc
    21661 ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga tactctcata
    21721 gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt ctttcttcat
    21781 tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt gaatctgact
    21841 taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct
    21901 gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc
    21961 tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat ttgcaggaga
    22021 tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt
    22081 catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat aagcgcctct
    22141 ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta ctaatgccga
    22201 atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct ccttcaataa
    22261 gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg tctgatccaa
    22321 acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct cacttgcttc
    22381 atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac cttgatattg
    22441 tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata tatccagaat
    22501 atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg aaatgtccca
    22561 gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc actaaatctt
    22621 gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct tcaatttcat
    22681 tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg tcagtagtca
    22741 atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga attttttcga
    22801 ataccataat aatatattcc tccgatattg tttgcttttt atttttataa tttaacagtc
    22861 tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt aaatattact
    22921 ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa taagataggg
    22981 atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt ttcttgcggc
    23041 atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg caaaagcaga
    23101 taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc tcgtacggca
    23161 aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga atcttctgaa
    23221 tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct ggtaccgtca
    23281 gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag aatcccacct
    23341 gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag cttagatagt
    23401 gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt ttcagccatg
    23461 cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat agcatgagtt
    23521 gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt
    23581 gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt tatcaatttt
    23641 tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct tcgttcacga
    23701 atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga aagatcatta
    23761 ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc aaattgttcc
    23821 attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag tgcatccagc
    23881 aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat ttcacgatga
    23941 aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa ctttacaacg
    24001 cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa cttgttaata
    24061 gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga agttgttttt
    24121 ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag atctttgaat
    24181 tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac actaacaaga
    24241 taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat tagtttgatt
    24301 atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa ccttaaaaga
    24361 tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca actgataaat
    24421 cagcatttat aatttctttt attacaaaaa tcatctatct cacatatttt gattatcaaa
    24481 gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt tcaaaatttg
    24541 ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac
    24601 tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg tcatctatgt
    24661 tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga ataaacagca
    24721 ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg tcgtagtaga
    24781 cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc gtccttttta
    24841 taaccgacaa atgaattatt tcagtaatta taattatgta catattacca ataagggatt
    24901 tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct ataaaaaaat
    24961 gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt ctgaaattag
    25021 tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg aacagaaaaa
    25081 aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt acttgaaaaa
    25141 aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga
    25201 tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt ttttaagaac
    25261 accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa agcactacct
    25321 cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt ctcctgactt
    25381 ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa aagaagaaat
    25441 cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac agtttgaaat
    25501 tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc tcggcttctt
    25561 ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta agtcatagat
    25621 ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt
    25681 taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta cgccttcttt
    25741 ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct ccagtcgttt
    25801 tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta tttctgttat
    25861 attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc gttttttatg
    25921 attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct tgggttccgt
    25981 cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg gtagttgctg
    26041 gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct ttgataaaat
    26101 gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa taattgctta
    26161 aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc
    26221 cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct gcaatcatga
    26281 tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat gtaaatgtcg
    26341 taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct ccaccgacca
    26401 tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt gcatctaacg
    26461 catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt ggtgtacttg
    26521 tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc gactgtccat
    26581 agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata ttactttgtt
    26641 ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa ggatcagctt
    26701 ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga tggcgtgcga
    26761 cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg acctcctaaa
    26821 aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt ttcataaaaa
    26881 atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc aatcgagtct
    26941 tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg ataaaattcg
    27001 atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc taaaactttt
    27061 ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg
    27121 gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata agcatttcct
    27181 tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc ttgtgtttct
    27241 gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac atcattgttg
    27301 tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat caattcctct
    27361 aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg ataagcggag
    27421 atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga aatcctgctt
    27481 aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa tttgctgata
    27541 tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt aggaatcacg
    27601 cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc acttggacaa
    27661 actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg ggattgcctg
    27721 atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg gtaagtatgg
    27781 atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc tccgaattct
    27841 gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc ataccacatg
    27901 cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg cattcgaatc
    27961 acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata gacttcatgc
    28021 ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc
    28081 ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa actagtcctt
    28141 gacctgattt acttttttta atttgctaga gactttttca ttcagtacac tttcgtatac
    28201 atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt gataaccggc
    28261 ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga taaattcttc
    28321 gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat acactggaat
    28381 atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga tcccttctgt
    28441 ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt ctatgatctc
    28501 tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta caattttccg
    28561 gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg tgtattcggg
    28621 catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa acaaaccgac
    28681 acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact tttgttcttt
    28741 cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt gaatcggcac
    28801 acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga tcagatagtc
    28861 cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct ggttcgatcc
    28921 gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa ctttttttcc
    28981 ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat tgatgtgcag
    29041 gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg tcagagcacg
    29101 tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt cactttcaaa
    29161 atataataat accttcacct tacgtccccc ttcacttata tagtataaca tagcctacaa
    29221 actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa cgttttctta
    29281 aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt tgatacgttg
    29341 tttattttac atattcatga acaagttcaa cgacttcttc catcgtatcg cattctttca
    29401 atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt aaactacgcg
    29461 ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct actagcaatg
    29521 gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt ccttcagcgt
    29581 gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat ggttggtaca
    29641 agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc aaatcgttcg
    29701 tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact gctgctgctg
    29761 gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct tcacttatca
    29821 atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg agtgtagcta
    29881 ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat gcacgcattt
    29941 gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca cgatatccta
    30001 agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct ccaccgatgt
    30061 ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct ttgtaagctt
    30121 catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat tcagtacggt
    30181 acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt ggtgttccga
    30241 tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca ttttttaatt
    30301 tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat tcagcttttt
    30361 gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc aaaatatcgc
    30421 cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt tctaaagaac
    30481 gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac gcttttacaa
    30541 aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca acgaccactt
    30601 cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct aaaatacgtt
    30661 tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct tccattgctt
    30721 cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca tttacgctgt
    30781 tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga accatcaaat
    30841 gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt tcacgaattt
    30901 gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct tcagccgcag
    30961 tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg taagcttttg
    31021 caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt attcagataa
    31081 gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt cagcaccttc
    31141 tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc ccatgattga
    31201 tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa atttgcttgc
    31261 tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta ctacgtgaaa
    31321 ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta tgtgagggtt
    31381 ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg taaagattaa
    31441 catttttttt cgttagatac aacagttttc attcaatttc agataaaagt attcaaatta
    31501 ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt atttcctttt
    31561 tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct ctttgttttc
//
LOCUS       DASJTM010000027        30799 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJTM010000027 DASJTM010000000
VERSION     DASJTM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.027"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(155..319)
                     /locus_tag="U4B29_001151"
     CDS             complement(155..319)
                     /locus_tag="U4B29_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106112.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            614..1942
                     /locus_tag="U4B29_001152"
     CDS             614..1942
                     /locus_tag="U4B29_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN2106113.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2144..2977
                     /locus_tag="U4B29_001153"
     CDS             2144..2977
                     /locus_tag="U4B29_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN2106114.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2993..3730
                     /locus_tag="U4B29_001154"
     CDS             2993..3730
                     /locus_tag="U4B29_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN2106115.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3885..4853)
                     /locus_tag="U4B29_001155"
     CDS             complement(3885..4853)
                     /locus_tag="U4B29_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN2106116.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5085..5918
                     /locus_tag="U4B29_001156"
     CDS             5085..5918
                     /locus_tag="U4B29_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN2106117.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5952..6791
                     /locus_tag="U4B29_001157"
     CDS             5952..6791
                     /locus_tag="U4B29_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2106118.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6817..7377
                     /locus_tag="U4B29_001158"
     CDS             6817..7377
                     /locus_tag="U4B29_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2106119.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7576..9297
                     /locus_tag="U4B29_001159"
     CDS             7576..9297
                     /locus_tag="U4B29_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN2106120.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9462..10604
                     /locus_tag="U4B29_001160"
     CDS             9462..10604
                     /locus_tag="U4B29_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2106121.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10620..11831
                     /gene="thiI"
                     /locus_tag="U4B29_001161"
     CDS             10620..11831
                     /gene="thiI"
                     /locus_tag="U4B29_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN2106122.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12404..13051
                     /locus_tag="U4B29_001162"
     CDS             12404..13051
                     /locus_tag="U4B29_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2106123.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13180..13396
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13444..16089
                     /locus_tag="U4B29_001163"
     CDS             13444..16089
                     /locus_tag="U4B29_001163"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN2106124.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16399..17712
                     /locus_tag="U4B29_001164"
     CDS             16399..17712
                     /locus_tag="U4B29_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN2106125.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17702..18355
                     /locus_tag="U4B29_001165"
     CDS             17702..18355
                     /locus_tag="U4B29_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2106126.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18410..19102
                     /gene="radC"
                     /locus_tag="U4B29_001166"
     CDS             18410..19102
                     /gene="radC"
                     /locus_tag="U4B29_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN2106127.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19252..19452
                     /locus_tag="U4B29_001167"
     CDS             19252..19452
                     /locus_tag="U4B29_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2106128.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19706..21385)
                     /locus_tag="U4B29_001168"
     CDS             complement(19706..21385)
                     /locus_tag="U4B29_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2106129.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21387..21599)
                     /locus_tag="U4B29_001169"
     CDS             complement(21387..21599)
                     /locus_tag="U4B29_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN2106130.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21992..23722
                     /gene="efrA"
                     /locus_tag="U4B29_001170"
     CDS             21992..23722
                     /gene="efrA"
                     /locus_tag="U4B29_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN2106131.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23719..25479
                     /gene="efrB"
                     /locus_tag="U4B29_001171"
     CDS             23719..25479
                     /gene="efrB"
                     /locus_tag="U4B29_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN2106132.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25574..25771
                     /locus_tag="U4B29_001172"
     CDS             25574..25771
                     /locus_tag="U4B29_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106133.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25924..26409
                     /locus_tag="U4B29_001173"
     CDS             25924..26409
                     /locus_tag="U4B29_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN2106134.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26513..27106)
                     /gene="clpP"
                     /locus_tag="U4B29_001174"
     CDS             complement(26513..27106)
                     /gene="clpP"
                     /locus_tag="U4B29_001174"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN2106135.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27286..27813
                     /locus_tag="U4B29_001175"
     CDS             27286..27813
                     /locus_tag="U4B29_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106136.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27904..28641
                     /locus_tag="U4B29_001176"
     CDS             27904..28641
                     /locus_tag="U4B29_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2106137.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28645..29562
                     /gene="miaA"
                     /locus_tag="U4B29_001177"
     CDS             28645..29562
                     /gene="miaA"
                     /locus_tag="U4B29_001177"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN2106138.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29564..30793
                     /gene="hflX"
                     /locus_tag="U4B29_001178"
     CDS             29564..30793
                     /gene="hflX"
                     /locus_tag="U4B29_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN2106139.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10172 a         5271 c         6574 g         8782 t
ORIGIN      
        1 gagtcctact gccggagtaa ataaagctta tgcaaagaaa aagaaatcca atcacttggg
       61 tttctttttc ttttattatt atgatgaatc aagctttctg tttttgtcac gtatgttgta
      121 atctttacga aagagtagag ttggctactc ataatcaacc atacttccca tcatcgctaa
      181 atacgaaata aatcagcatt cctacaccaa tcaaagcaca agctgcacca agaaaaggga
      241 tgacattccc aaagttatct gtattcataa gtatgccacc acccacaaac aaaaagccgc
      301 cagcaatcag gtttttcatt ttattcatct cctttcttac gttatagtat ttccttcttt
      361 caatgagtat tttgaaagct atcttattgt atgatgttct tctaggcctc ttctttatta
      421 tacaggccat tttcgttatc tcatagaaat attccttttt atctgaaaac tattgtatac
      481 cgtatgacat ttttcttcat gaagttgtaa gtatattctc attcaaatga aaatttcatc
      541 atagaagaga ttgtatttct aaattcttaa gagtaaaata cgaagtataa cttggatttg
      601 gaaggatgat tggatgaaag ctattacgcc tgatatgaca acaaaattta acaaacagtt
      661 tcaagcaaat atcaaacagc aggcattgca gcgggcagtt gtgaagaacg gaattactgc
      721 ttctgctgaa aatgtttcag cacatgtgga gaatacaccg gtattttcta tagatttggc
      781 tacggggaaa gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa
      841 cacattccgt cataagatgc tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa
      901 ttacactttt ttctgggata aatatgaaaa ggcaaattat ttctatgaaa acatcattgc
      961 aacaggaaaa gaggcaatcg atagccggaa agtagctttc ttactcgcta ctcctcaaca
     1021 agatggcggc caatgggata tgatcgtttc attattccaa aaatatggcg ttgtgcctaa
     1081 atcggtgatg ccggaaagtg cgaatagttc aaattctcga gatttgaata actacttgaa
     1141 caaattactg cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag gagaaacatt
     1201 agacgctatt gaagaaaaga aagaagaaat gctagaaagc atttataact tcttagctat
     1261 ctcattagga acacctccaa aagaattcga ttttgagtac cgcgatgaag aaaagaacta
     1321 tcatcttgac cgaggactga caccacaaat tttttatgat aaatacatcg gtgtaaaatt
     1381 agatgattat gttagcgtga tcaatgcacc aacaaaagac aaaccattca atcgttctta
     1441 tacagtagag atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga
     1501 tatggaaact ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt
     1561 tggttgtgat gtaggtcaat cttctactag aactagtggc atcatggcct tagatgctta
     1621 ttcaatggat gatctatttg atacggattt tacaatgact aaagctgaac gtttagattt
     1681 tggagaaagt ctgatgaccc atgcgatggt attgaccggt gttgacttag tagacgggca
     1741 atcaacaaaa tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga atggcttctt
     1801 tgttatgagt gatgcctgga tggacgaata tacgtaccaa atcgttgtaa gaaaagattt
     1861 attgacgaaa gagcaattaa atgcgttcaa tgaagaacca attgttctat ctccgtggga
     1921 tccaatgggt gctttagcat agacgagtat ttcaatgaag agtatgtagt aaagacaaaa
     1981 agagctggct taagccagct ctttttatct ttaaagtcca ttccctgttt tttatggtcc
     2041 ctgtgctata attctaaata aaggataagg attggaaata atccttttat cattaaaaat
     2101 atgatgacga agtagaataa aattgggagt aggggggaaa gtcatggata tccaaactgt
     2161 tttatctgat ctgaaattaa atggtaaagt gattccagtt gttggtggcg atgttaatca
     2221 gacctatcgt ataaaaaccg aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa
     2281 aaaaggattt tttgaagcag aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt
     2341 accggatacg tatatgctag gtgaaacaag tgaaggcgct tatttgttaa tggaatggat
     2401 cgagccggga aaaggggatc aacgagatct tgctgcagct ttagctaatc tccatcagca
     2461 aacagcgcca caatttggtt ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa
     2521 ttcttttgaa gaagattggt ggactttttt ctttaaagat cggcttgaat cccagatttc
     2581 tttggcagaa gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa
     2641 agaacgtgta ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct tgcacggaga
     2701 cctgtggagt ggtaatgtgt tctttgacca gcaagggcat ccagtttttg tcgacccagc
     2761 tgtttcttat gggaatcggg aacaagatat tgcaatgagc cagttattcg gtggtttccg
     2821 accggaattt ttagatgctt atcaaactat atttccatta gaaaagggat ggaaagaccg
     2881 actgccgatt taccagttat attatctttt ggcacatttg aatatgtttg gagaaagtta
     2941 cggttcccaa gtagatcaat tactagaaaa tttttaagag gagggcgtgt ttttggcaaa
     3001 aaaatatacc aaaaagcatg aagtttcata ttatgagtgt gacatcaacc aaacgatgac
     3061 atttccttcg atgttaggag tagttatcaa aacgtctgaa gatcaaagcg accagttggg
     3121 aagaggcagt gaattcgtca atagctttgg acttacttgg gtcatcacga attattccat
     3181 ggagattaat cgtctcccta gagtcggtga agtaatctca gtaacgactc aagccatgga
     3241 gtacaataag tttttctgtt atcggaactt ctggatccat gatgaagatg ggaaagaatt
     3301 agtcaaaata gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt
     3361 taatgaagag atcattgctc cttttgagag tgagaagatc aagaaaatca agcgtcagga
     3421 aaaaattgaa aagattgatt cgggcttcat gttgccatat cgtgttcgtt tttatgatat
     3481 cgatagtaat cagcatgtta ataatgcgat gtattttaat tggatcattg acgtattagg
     3541 atatgacttt ttgactacgc atgttccaga aaaagtaatt attcgatttg ataaagaagt
     3601 agagtacggg aatgagatcg aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa
     3661 aacaaaacat gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata ttatatggaa
     3721 aaataaataa aaaagaggtt gtgacagaag ggggcaactt caagaaataa gaagaaattt
     3781 acgaaaattg tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt
     3841 tgtcacagcc tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac
     3901 gaaatcaatt cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct
     3961 ttttgactat taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg
     4021 ccttcataat cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca
     4081 gggatttttt ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc
     4141 gcacctaaag cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag
     4201 tccccttgca tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg
     4261 tccagtcgct ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag
     4321 ccgatgggac ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc
     4381 acgtagcgtg cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct
     4441 tcttcacaac ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt
     4501 tcattacttg aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta
     4561 tcaagaaaaa aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat
     4621 atacctgatg cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg
     4681 atgtgtggtg agggaagatt gaagatatct tcaactaata attctacatg cttggggatt
     4741 gtgatttctg catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg
     4801 ttttctttca tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag
     4861 acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt
     4921 tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta
     4981 aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt
     5041 tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat
     5101 taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat
     5161 atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt
     5221 cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct
     5281 atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga
     5341 ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt
     5401 tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac
     5461 ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca
     5521 gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca
     5581 tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc
     5641 cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata
     5701 aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag
     5761 cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata
     5821 attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc
     5881 cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag
     5941 gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc
     6001 gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt
     6061 tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg
     6121 aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta
     6181 tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc
     6241 atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag
     6301 gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc
     6361 tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt
     6421 tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga
     6481 aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag
     6541 atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg
     6601 aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga
     6661 aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag
     6721 actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc
     6781 tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa
     6841 aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt
     6901 acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt
     6961 gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca
     7021 ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt
     7081 gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt ttcattacta
     7141 ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt
     7201 ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg
     7261 cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa
     7321 gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca
     7381 gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt
     7441 agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg
     7501 tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa
     7561 acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata
     7621 gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg
     7681 gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat
     7741 gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa
     7801 aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg
     7861 gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa
     7921 gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg
     7981 cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa
     8041 ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa
     8101 aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga
     8161 gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa
     8221 gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa
     8281 cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa
     8341 gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat
     8401 cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt
     8461 gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac
     8521 caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt
     8581 gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga
     8641 ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc
     8701 cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc
     8761 ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa
     8821 ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa
     8881 cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt
     8941 gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg
     9001 gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag
     9061 acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt
     9121 tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat
     9181 aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa
     9241 cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc
     9301 aaaaaagtta gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt
     9361 gtatatttga aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa
     9421 ccgttataat agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca
     9481 gtgcaacaac cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga
     9541 ttattgggaa tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc
     9601 aagcaagaaa gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt
     9661 caggcggaac agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat
     9721 atgggaatca tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac
     9781 aattaaaaga attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac
     9841 gtgtcgacga attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg
     9901 tgaataatga agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt
     9961 ttcctaaaat tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc
    10021 aatggttgac agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac
    10081 ggggaactgg tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg
    10141 gagggcaaga aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg
    10201 ttaaagcttt acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct
    10261 taaaagtagc tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag
    10321 gaactgacta tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc
    10381 tcgtccatgc cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac
    10441 gtaaacatat ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat
    10501 ctgctgttcg aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga
    10561 ttattttcaa gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg
    10621 tgaaatacag tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa
    10681 aaagctttat catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa
    10741 tcaaaatcca tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc
    10801 tagttattcc aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg
    10861 tagaaaaaga tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata
    10921 ctgggaaaga aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa
    10981 gtaatgaatt gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag
    11041 ttcaaatgcg tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc
    11101 tatcgtatga aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa
    11161 tgcttatgct ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg
    11221 gagtggaaat tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc
    11281 aaaaagcaaa agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg
    11341 aagtgccatt taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga
    11401 tgacaattac tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg
    11461 gtttagtcat tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta
    11521 tggtggctat caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata
    11581 aacttgaaat cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt
    11641 ttgaagattg ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa
    11701 aggtccatca atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg
    11761 gcttgaagat ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag
    11821 aattgctata agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga
    11881 aaaaatagct ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat
    11941 accatttatc aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt
    12001 ttatttagag atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat
    12061 agcgaggttg tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc
    12121 ttttcaaatt tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt
    12181 gtatcagagg ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
    12241 agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc
    12301 gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac
    12361 atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga
    12421 tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct
    12481 ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc
    12541 ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg
    12601 ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa
    12661 acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag
    12721 aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg
    12781 agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa
    12841 aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga
    12901 aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc
    12961 agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt
    13021 tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc
    13081 gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg
    13141 accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg
    13201 agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa
    13261 ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat
    13321 cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa
    13381 atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac
    13441 agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga
    13501 cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa
    13561 ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct
    13621 tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact
    13681 ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag
    13741 cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg
    13801 tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta
    13861 tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt
    13921 aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac
    13981 tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa
    14041 ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg
    14101 acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag
    14161 cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca
    14221 atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca
    14281 gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt
    14341 atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt
    14401 gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat
    14461 acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg
    14521 tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa
    14581 acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg
    14641 gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca aattcctgca
    14701 tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa
    14761 aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc
    14821 tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc
    14881 actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc
    14941 ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac
    15001 gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc
    15061 gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa
    15121 gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg
    15181 aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta
    15241 tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa
    15301 cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac
    15361 tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga
    15421 gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc
    15481 ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga acatattcct
    15541 catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat
    15601 gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt
    15661 cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat
    15721 gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc
    15781 gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg
    15841 acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa
    15901 cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt
    15961 tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa
    16021 gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact
    16081 attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg
    16141 attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga
    16201 tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag
    16261 ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt
    16321 ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga
    16381 gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta
    16441 ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat
    16501 ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta
    16561 gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct
    16621 tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat
    16681 ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca
    16741 gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg
    16801 aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat
    16861 gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt
    16921 ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca
    16981 gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca
    17041 aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat
    17101 gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc
    17161 actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt
    17221 gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag
    17281 aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat
    17341 ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa
    17401 ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta
    17461 gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa
    17521 gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg
    17581 tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg
    17641 accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat
    17701 catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt
    17761 gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct
    17821 gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt
    17881 ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct
    17941 tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt
    18001 tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga
    18061 acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa
    18121 tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac
    18181 agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc
    18241 ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa
    18301 aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc
    18361 catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg
    18421 attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc
    18481 ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta
    18541 tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg
    18601 acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga
    18661 aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc
    18721 accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa
    18781 acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac
    18841 agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc
    18901 tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgttat
    18961 tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg
    19021 gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga
    19081 aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat
    19141 tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac
    19201 gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac
    19261 ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga
    19321 agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa
    19381 ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt
    19441 gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga
    19501 acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg caggtatttt
    19561 tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg tatcaaggaa
    19621 taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat attcaggaca
    19681 aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc attggcaaaa
    19741 tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt
    19801 ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata
    19861 agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca aatccacgag
    19921 agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc gcgacagcta
    19981 atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta ccaatatcac
    20041 cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca ggacgcgcag
    20101 aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt tctgcaggaa
    20161 cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct ccgtggacag
    20221 gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct tgtccaccgt
    20281 gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca gcttcagaca
    20341 gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa cttgaaaaga
    20401 tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg atgcggctca
    20461 acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca ttggcaggca
    20521 agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata tatcctaaac
    20581 gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag
    20641 ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca aagctggcaa
    20701 aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca atcgtttttt
    20761 ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg cacaagactc
    20821 cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca ggagtgaaat
    20881 cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc acgatcccta
    20941 atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt ctaaaacgga
    21001 tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca tgctcatcta
    21061 atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata tttgcttgtt
    21121 tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt
    21181 ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag tcaattccaa
    21241 gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca tcttggaatt
    21301 gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag acgcctgtct
    21361 cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc agcattttct
    21421 ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt gtatggggta
    21481 ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta aagagatttt
    21541 gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt atagatcatt
    21601 gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt ttagacaaca
    21661 caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata ctggattcga
    21721 gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa ccgtaccttc
    21781 tgtataaaaa atatacaaca attaattgtt attctagcat aaaacagcaa ataagtatag
    21841 aaatcaacta gattgaaaga aaaaatcaat gtttatcatc ccgctataat cataatgact
    21901 tttttatgag agaagtgttc tattaatagg ctcgttgtgc tatcattttt agtaatgaaa
    21961 atgtaacaaa agattaaaag gagatgagaa agtgaaacta atgtggcatt ataccatgcg
    22021 ttataagaaa ttcttgtttt tcaatttcat atgcgtattt ggttttattt tgattgaatt
    22081 aggacttcct acgattttag cacgtatgat cgatgtagga atccgcaatg atgattttga
    22141 ttatgtgaag caacaaggga tatttatgat cgttattact gtaatcggta ttattttgaa
    22201 tatcttgtta ggttattttg gttcgaggat tacgactaat atcgtagcag atattcgaga
    22261 tgatttgttc gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc taggcgtatc
    22321 ttcattgatc actcgtacaa caaacgatgc ttatcagatc atgttgttcc tgcaaaatat
    22381 tttacgtatc ggttttatgg caccaatgat gttcattgtc agtttgtaca tggtcatgcg
    22441 tacaagtgtg acactcagtt tgtatgtagt aggtgcatta cctttactat tattggcagt
    22501 cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa acttagataa
    22561 aatcaacagt atcttgcggg aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa
    22621 tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag aattatgctt ctagttcaaa
    22681 aagcttattc cgtttgatgg ctgttgcaca accgggtttt tatttcttat ttaatatcgt
    22741 aatggtattg atcatctgga gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg
    22801 agatttgatt gcgtttatcg agtatatatt ccatgccttg ttctcattca tgttatttgc
    22861 tagtgtgttc atgatgtatc caagagcagc tgtttcagct ggacgtatcc aagaagccct
    22921 agatgccaaa ccttcgattg tagaagatcc aaatggtata tctgatacaa aaactaaagg
    22981 gtatatcgaa tttcaaaatg taacatttgc ttatccaggc cattcgcaag agccagtgat
    23041 acgaaatgtc agttttacag cctctccagg agagaccgtt gcttttattg gaagtaccgg
    23101 tagcggaaaa tctacgttga tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa
    23161 gatcttgatc gatggagtag acgtgagaga ttatcggcta agcaagttgc gagataagat
    23221 tgggtttatt ccgcaaaaag cgctgctatt tactggaacg attgcagata atttgcgata
    23281 cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca gctgagattg ctcaagcttc
    23341 agaattcatt tctcgaaaac cagatggatt taatgaacat ttatcagaag gtggttccaa
    23401 tttttcaggt ggacaaaaac aacgattggc aatagccaga gccatcatac gccgtccgga
    23461 aatctatatt tttgatgaca gcttttctgc attagattat caaacggatg cgaagttacg
    23521 tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg atcgtagcac aacgtgtggg
    23581 aacgatcatg catgcagata agatcatcgt attaaatgaa ggagaagtcg tcggtatggg
    23641 gacgcatcgg gagttgttgg aaaaatgccc gatttattat gatattgccg cttctcaatt
    23701 atcaaaggag gaattagcat gaaaaaaacc ttttcttcct taaaacgttt agctcgatat
    23761 atcagaccgt atcgtgagac ttttctttta gtgatcctat ttacagttct gacagtggct
    23821 ttcaacgtgg ctttgccata tgttacagga cttcctacta cagaaatcag ccgtaatatt
    23881 gccaatggcg aatcaatcaa tttttcttat atattcaaat gtctcatttg gatcacagtt
    23941 gttggaatag ggtattgtat ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt
    24001 caaagtgcca tgcatgattt gagaagagac atagaagaaa aaatcaatcg tcttccggtt
    24061 tcatatttcg ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat
    24121 gcagtcagcg gtgctttaca acaagctttt attggtatcg taaatgcgat attgggaatt
    24181 acgttggctg cagcaatgat gttttatatc cagccgatga tggcgctgat ctctatgatc
    24241 atgattccaa catcgatctg gatctcaaaa cgagtgatca atgcttcgca gaaatatttt
    24301 caaagcatgc agaattctct tggtgagcta aacggctacg tccaagaaaa tatgacaggt
    24361 tttagtgtat tgaaagtata tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt
    24421 aatcacagct tgaaatactt tgggtttcgt gcagcattca tttctggctt gatgatgcct
    24481 ctagttcaat taactgccta tgctacttat attggtatgg ctgttctagg aagcttttat
    24541 gttattactg gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata tatttggcaa
    24601 atcagtcagc caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg
    24661 gcaacgaaac gtgtattcga gattttagat gagccagaag aaaaagaaaa caaagtagat
    24721 gcttcactgc cagaaacgat tttaggagat gtgacattcg atcatgtcga ttttgcatat
    24781 gatccgaaga aaccattaat ccaagatttg aattttgaag tgaaagctgg tcaaaccgta
    24841 gctgtggtcg gtccgaccgg tgccggcaag acgacattga tcaatctatt gatgcgtttt
    24901 tatgacgtag acaaaggagc aataaggatt gatggaatcg acaccaaatc aatgagtcgc
    24961 agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg catggcttta tcaaggaacg
    25021 attgcagata atatccgctt cggaaagctt gatgcgacag attatgaagt agttgatgct
    25081 gcgaaaacag caaacgtaga tcattttatc cgaacgatgc cagatggata tgatatggcc
    25141 atcaattctg agggagacaa tgtgtcttta ggacaaaaac agttgctgac gattgcccga
    25201 gcagtggtct ccgatcctaa aatcttgatt ttagatgaag caacaagttc agtagacacc
    25261 cgtttagaag cgttgattca aaaagcaatg gatcgcgtga tggagggacg gacaagtttc
    25321 gtcatcgctc accgtttatc gacaatccgt gatgcagatc tgattctagt gatggatcaa
    25381 ggtcagatca ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg attttatgaa
    25441 aaactgtata acagccaatt tgctgaggaa gcagaataag tcatataaga ataaaacagt
    25501 ttaaaactta ttttttgttc tctattcttg ataatggtaa agtaaagtta tcaagaaaag
    25561 tgaggacacg gagatggaac agtttttaaa tgaaaaaatt actgaagcca aaagagaagt
    25621 tgatcgttta acgcacgtct gtcagacgaa gccttttgat tcagctgatt tttttgaaaa
    25681 tgagtttcaa atcgaacgct tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa
    25741 agtaatagaa gaaaatatag atgtacaata aaaacaggta aaacgtcagt taccagatac
    25801 tgtaaataga catctggcct gagcaggttt tacctgtttt ttttcttatg aaataaatat
    25861 atgctatgat acctttattc aataggatta gtttctacaa aaaatgaaag gagaaaaata
    25921 aatatggaac ggtttatagc atcaaatgcc acagtgattg gtgatgtgac attgtcagaa
    25981 gatgtgacga tctggtatca agctgtttta cgcggggaca gtaattggat aaaaatagga
    26041 caaagaacga atatccaaga tggtacaatc atccatgtcg atcatgatgc acctgtagat
    26101 attgcggaga atgtgacggt aggacatcag tgcatgctac atggctgtac aatcgaaaaa
    26161 ggggcgctga tcgggatggg gaccacgata ttgaatcatg cggttattgg tgagaatagt
    26221 ttgattggtg ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct
    26281 tttggtcgcc cagctaaagt gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa
    26341 gaaaacatcc agcattatgt cgagataggt cggatgcatg cgcaaggaaa attcaaggag
    26401 caaagatagg cagtagcatc cataaaataa aaaaggatgg tcagtttgag ttgaccatcc
    26461 ttttttattt ggtttagata agagagctta aaaaagcttc ctttgaaaca gattaattta
    26521 atgaagaact gttttccatg acttcatcga tcaatccata tgcttttgct tgttcagctg
    26581 tcatatagtt gtcacgatct gtatcttttt caatcacttc aagcggttgt ccagtgcgtt
    26641 cagctaaaat tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga
    26701 tttcagttgc ttgcccttga gcgccgccaa gaggttgatg gatcatgatc tcagcatttg
    26761 gtaaagcaaa tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc attgaagcag
    26821 ccatacccat aacaattgtt tggacatctg ctttgacaaa gttcattgtg tcgtaaatag
    26881 ccatacctgc tgtaactgaa ccaccaggag aattgatata aagatagata tctttttcag
    26941 aatcttgagc gtctaagaaa agcaattggg cgatgatcga attagcaaga tcatctgtta
    27001 cttggccgct taacatgatg atacggtctt tcaataaacg tgagtagata tcgtaagctc
    27061 tttcacctcg tgaagattgt tcaatgactg taggaattaa attcataata gagttcctcc
    27121 ttgatttcgt ttagaaatag tgtaccataa tttagtttct aatacctaaa atgtagtata
    27181 catttttggt caaaaaaggt caaataaaaa acttaggcaa attagtcgca taaacccgct
    27241 ttatggtatg ataagcagag aaacaaaaca aggaggaaca acagattgat agttggtatt
    27301 actggcctta tcggagtgct gacaggtgct gtagttgtct atatcggcat gatactgcaa
    27361 atgcgtcact tggaaaataa agaacagcgt agacgtgaat tggaattgaa agtcaaagaa
    27421 atcgagacgc tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa acggacaacg
    27481 ctgatggaag aatatgtcag ctttgatgca tttgatgatt gttatatcac gattgacgat
    27541 tttgcgtatc tccaatcatt ttcagcgcaa aatagttttt atctgccaaa ttatatttta
    27601 gacgagctat ttaagaatat ctcccatcga aaagtgattt tatctccaga tgaaacaatg
    27661 aagattggtg gatatgctta taaaggcgga cgcgtgattt tagagaattt tacggatagt
    27721 ctgactgaaa tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt
    27781 ggtttcttct cgaaagatga tgtttggagc tgatattttg aagcattgcc gttgcacatg
    27841 agagcgtggt aatgctttct ttttttctta atgatacaat agaactgaac ggagtgaaga
    27901 aagatgttac aaatctatgc ccatcgcgga agcagcggca cccacccaga aaatacgctt
    27961 cctgcatttg cagaagctgt acgagtaggt gcagatggta tcgaactaga tgtccatcta
    28021 agcaaagatg gttatttgat cgttatgcac gacgaagaag tggaccggac gactaacggt
    28081 aaagggctga ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc agggggcaaa
    28141 ttcagcaaag aatttccagc agctaaagtt cctgaattga gagaagtgtt aggactgctc
    28201 ctccaaaaaa attaccgagg gatgctaatg attgaaatta aaactgatca gtatgaatat
    28261 cccggtatcg aagaaaaatt agccgaaaca ttaaaaagta gagattggcc ttttcaacat
    28321 gcatattgca gctttcattt ggattctttg gaaaaaatcg cattactgga accggagata
    28381 cagctggact tattgatgag tacttctgat aaaaagatcc aactagcagc agaaacccct
    28441 tatatagaag gagtacatcc aaaaaatact tggttgttcg aacatccagt ccaggcagct
    28501 gattttccaa aagcaattcg tgcatggacg attaacgatg aagaagaaat ggccaaatgt
    28561 caagaactca aaatagatgg aataataact gattttccgg agaaagctct tcggtggaaa
    28621 caatcaacga aaagtaggta aaatatggaa aaagtattag tgatcgtcgg tccgacagca
    28681 gtcggtaaaa cagcgctaag tgtggaactc gcacaactat ttggaggaga gatcatcagt
    28741 ggtgattcat tgcaaatcta taaaaatctg gatatcggta cagcaaaaat caaagaagaa
    28801 gagatgaata atgttccgca tcatttgatt gatgtgatcg aacctactca acgttactca
    28861 gccgttgatt ttcagcattc aggtcgacaa ttgattaccg agattgcgaa ccgagggcac
    28921 ctacctatca tagctggtgg tacgggtctc tatatccaat cccttctata tgattatcag
    28981 ttaggagcga cagaagaagc gaatcctgag attagaaaaa agtatgaaac gttagctgaa
    29041 acaattggtt cccaagcatt atggaatcag ttgaaagaaa aagacccggc agctgctgag
    29101 aaaatccact ggaacaatca acgcaaagtg ataagagctc tggaagtttt agagacgaca
    29161 ggacatagta tcacggcacc taaagaagag ccaaagcaat tgtatgatta cctcatgatt
    29221 ggattagata cgaagcgtag tattctttat gaacgaatca atcaaagagt cgatttgatg
    29281 ctagcagaag gtttagtagc agaagcgcgt aaggtgtatg agcttggaga agtccaggcc
    29341 agtcaaggta ttggatataa agaatttttc ccttattttg aagggaaaga aacattggaa
    29401 gaagcaacag aacaagtcaa actacattcg agaagatacg cgaaacgtca gttgacgtgg
    29461 tttcgtaacc gtttggatgc acagtggttt gatcttataa gtgagccaaa tcaaaaaagt
    29521 cagattgagc aaacaatcaa aaaatggctg gaggcggaat aaaatggaag aaaaagtgat
    29581 catcgtaggg gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa tggaagagct
    29641 agttcagctg acgaataccg catcaggaga agtcgtattc acaattacac aaaagagacc
    29701 acaagtggat cggcaaacga ttatcggtaa agggaaatta caagaattag ttcaacaagc
    29761 ggatgcgcat gaagcggatt tgatcatctt taatcatgag ctaactccga gacagagcca
    29821 actgattacc gatgctgttg gtcttccggt aattgatcgc gtgcagctta tcttagatat
    29881 ttttgcgatg cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg ctcagttaga
    29941 atacctgctg cctcgtttag tagggcaagg gaaaacatta tctcgtcttg gcggtgggat
    30001 cggaacaaga gggccaggtg aaacaaagtt agaaacagac cgccgacata tacggaataa
    30061 aatttttgct gttaagaaag aattgaaaga agtagaagct catcgagagc gcaatcgcca
    30121 aaaaagaaaa aatagcgaga tttttcagat tggacttatt ggctacacga atgccgggaa
    30181 gtcaactatc ttgaatctat tgacacaagc ggatacttat tcgaaagatc aattgtttgc
    30241 aacgttagat ccattgacta agagatggcg ttttgctgaa ggatttgaga ttactgtaac
    30301 ggataccgtc gggttcatcc aagacctgcc tacgcaactg atcgatgctt tccattctac
    30361 attagaagaa agtcaaaata tggatctttt gctacatgta gtcgatgcta gttctccaga
    30421 tcgtatttta caggagcaga ctgtcttaaa attgatggat gaactgaata tgaaagaaat
    30481 gccgatactg actgtttaca ataaggcaga ccagatcgac ccagcgatgt tcacgcctac
    30541 gctattccca aatgtattga tttctactca gacgaaagaa ggtaagatgg cgttgatcga
    30601 agcaatcaaa cgtcagctga tggaactaat gaccccttat acactttctg tgccaagcaa
    30661 cgaaggacag aaattaagcg agttgagaag acaaacaatg gttttggacg aagcgtacat
    30721 ggaagaaaca aacgaatatg aagttaaagg gtttgcaaaa accaattcaa aatggttaag
    30781 aaaaaaccaa taaaatttt
//
LOCUS       DASJTM010000028        30218 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJTM010000028 DASJTM010000000
VERSION     DASJTM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.028"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(209..1519)
                     /locus_tag="U4B29_001179"
     CDS             complement(209..1519)
                     /locus_tag="U4B29_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2106140.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1531..2889)
                     /locus_tag="U4B29_001180"
     CDS             complement(1531..2889)
                     /locus_tag="U4B29_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2106141.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3091..4659)
                     /locus_tag="U4B29_001181"
     CDS             complement(3091..4659)
                     /locus_tag="U4B29_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2106142.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4663..5073)
                     /locus_tag="U4B29_001182"
     CDS             complement(4663..5073)
                     /locus_tag="U4B29_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN2106143.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5070..5993)
                     /locus_tag="U4B29_001183"
     CDS             complement(5070..5993)
                     /locus_tag="U4B29_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN2106144.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5990..6868)
                     /locus_tag="U4B29_001184"
     CDS             complement(5990..6868)
                     /locus_tag="U4B29_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN2106145.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6855..8192)
                     /locus_tag="U4B29_001185"
     CDS             complement(6855..8192)
                     /locus_tag="U4B29_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2106146.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8195..9298)
                     /locus_tag="U4B29_001186"
     CDS             complement(8195..9298)
                     /locus_tag="U4B29_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2106147.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9372..10880)
                     /locus_tag="U4B29_001187"
     CDS             complement(9372..10880)
                     /locus_tag="U4B29_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2106148.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10846..11538)
                     /locus_tag="U4B29_001188"
     CDS             complement(10846..11538)
                     /locus_tag="U4B29_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2106149.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11560..12534)
                     /locus_tag="U4B29_001189"
     CDS             complement(11560..12534)
                     /locus_tag="U4B29_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2106150.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12834..13706
                     /locus_tag="U4B29_001190"
     CDS             12834..13706
                     /locus_tag="U4B29_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2106151.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14164..16362)
                     /locus_tag="U4B29_001191"
     CDS             complement(14164..16362)
                     /locus_tag="U4B29_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106152.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16594..17586
                     /locus_tag="U4B29_001192"
     CDS             16594..17586
                     /locus_tag="U4B29_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2106153.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17646..19067
                     /locus_tag="U4B29_001193"
     CDS             17646..19067
                     /locus_tag="U4B29_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN2106154.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19060..20559
                     /locus_tag="U4B29_001194"
     CDS             19060..20559
                     /locus_tag="U4B29_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN2106155.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20598..21992
                     /gene="uxaC"
                     /locus_tag="U4B29_001195"
     CDS             20598..21992
                     /gene="uxaC"
                     /locus_tag="U4B29_001195"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2106156.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22151..22834
                     /locus_tag="U4B29_001196"
     CDS             22151..22834
                     /locus_tag="U4B29_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN2106157.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22836..23672
                     /locus_tag="U4B29_001197"
     CDS             22836..23672
                     /locus_tag="U4B29_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2106158.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23683..25437
                     /locus_tag="U4B29_001198"
     CDS             23683..25437
                     /locus_tag="U4B29_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106159.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25713..26054)
                     /locus_tag="U4B29_001199"
     CDS             complement(25713..26054)
                     /locus_tag="U4B29_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2106160.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26122..28293)
                     /locus_tag="U4B29_001200"
     CDS             complement(26122..28293)
                     /locus_tag="U4B29_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2106161.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28462..29361
                     /locus_tag="U4B29_001201"
     CDS             28462..29361
                     /locus_tag="U4B29_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2106162.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29432..>30218)
                     /locus_tag="U4B29_001202"
     CDS             complement(29432..>30218)
                     /locus_tag="U4B29_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2106163.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9039 a         6229 c         5355 g         9595 t
ORIGIN      
        1 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttcttttta acgacttagt
       61 tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat aaaatgtttc
      121 aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc aagctaaaat
      181 attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg tttgcagctg
      241 tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt actgcttcga
      301 ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct tctataggct
      361 tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct ggagaaaggc
      421 atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt gattcaggta
      481 tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg ctattttttc
      541 tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca tcaatcatat
      601 tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa gatttcattc
      661 gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca ctaactccag
      721 cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat ctagcaacct
      781 cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca gacttaatag
      841 caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt gttgaagaat
      901 caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact ttttctacta
      961 atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga aaaatcaaat
     1021 cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt ttaatagcat
     1081 aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg cttttttgat
     1141 cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt ccttttcctt
     1201 ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat ttcatttcta
     1261 cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc cccttgagga
     1321 ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct ccgactaaat
     1381 aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg attgcttttt
     1441 gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa gcattgataa
     1501 tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt tcaattcttc
     1561 accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa tgattgcgga
     1621 tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt cttgtactaa
     1681 aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg ccagcataaa
     1741 tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat ttactccata
     1801 tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca cgtcagtaat
     1861 gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa ataaaagaat
     1921 cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca tacatgaaga
     1981 agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca gctttgtagc
     2041 aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag acattgcttg
     2101 tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta aaatctgatt
     2161 gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag ctaacaaacc
     2221 ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc tttcagtagt
     2281 caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga ttgaaatcca
     2341 aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat acattaaaat
     2401 cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc ggaacgtgga
     2461 caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc catacgggat
     2521 attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat aagcaatctt
     2581 caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca ctaagcaaaa
     2641 tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta actctgagac
     2701 atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg aaatcaagaa
     2761 taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa tcatcaagaa
     2821 actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt agcctatctt
     2881 atcggaaatc ccaaattcct ttttttcttg tttttatatt cacaatgttg gctttttatt
     2941 gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc ataagattga
     3001 taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag tgaaaataag
     3061 ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat catacttgaa
     3121 aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg ctatcccgat
     3181 aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac ctactttctt
     3241 ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct ttacagtgac
     3301 tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc
     3361 gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag taacataaca
     3421 ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat taaaattatt
     3481 aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa
     3541 caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta aaaaacctgg
     3601 tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat tccagttctt
     3661 ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt gtacctcttc
     3721 atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa gtcttaacca
     3781 atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa tagccgcacc
     3841 gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg tttgtactaa
     3901 gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg tcagcatagg
     3961 aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa ctgtaactga
     4021 atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt tacgaaaata
     4081 caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat agatgggacc
     4141 atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg atttattcgg
     4201 tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg tttcaagatc
     4261 atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg accaaggacc
     4321 tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac ggcctgcact
     4381 tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct gtggcgccca
     4441 tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa tatccgttgt
     4501 atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa gccattcaaa
     4561 agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat ctggattgaa
     4621 tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc cttttctcat
     4681 tcttactcga tataaactga atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc
     4741 aataaactga gtgccaatca acaatatgaa tcctgttata aaatacaaac cagtggcagt
     4801 tgttaagtat tttcctttag cagtcaaaaa caatgctact gctaaaaaag caagtgaaat
     4861 aaacaacaca acggcaaaca gaccagtcat gtcctgtaat aatccaaatg tattttctaa
     4921 agacaaagtt gaatgatcta cagcaagcga ctgataactt tttttaaact ctgacgcaga
     4981 catattactt aaagtaactg ctaacccgcc aaagaaaatc aaagatagac tgccaccgac
     5041 cattcctagc aatagttccc attttctttt catttctgaa ccccctgctt ttctaaatgt
     5101 atccattcag gtcttttgat cgatgtttca taattgttat tacgttcaga gaatttgaca
     5161 gtctgttcat ttttcttgtc atcccaatta tcccaaccag ttgggtgaac atgcccacct
     5221 atcttacacg cttcaaataa gacatttgca tacggacgcc acggtcttcc taaatagacg
     5281 tttctagtat caggttctgc agtcaaggta cagctttcaa aacgataccc tgttttttga
     5341 tttttcggcg tagatgctgc tgtgatgtat ccaggatttt tactggctaa gctatggata
     5401 tgacactgat gaaaaacggc atctgcccca ccaaatatga aatccaccgt tccttcaatc
     5461 cagcaatatt cataggtctg acgacaatac tcaggattgg cagaagccgg tgtacaaaaa
     5521 ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc ctgataacca
     5581 gatagtcgac agttttttaa agatacgcga tctcctgaat tgaataaagc aacagcctgt
     5641 cctacctttt ctcctggacc tgctgtattt tcgatgtgaa gattttctaa tacgacattc
     5701 gatccttcta agaaaaaagt agcacttcgg aaagtcccta actggctacc gtcaagatac
     5761 tcctgttttg caaaacgatt accggagata actactgttc ctgctcccac aagtttaaaa
     5821 ttggataatc tgctttctat atattcatca tatcttcctt cttttactat caagaccttt
     5881 ggatggctat ccggcaggcc tgccaaataa tcgattcctt cttggatagt ttcaaaatcc
     5941 gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct cataaatgct
     6001 cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga ccgcctgtta
     6061 aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg tccagccctt
     6121 ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca actaagctaa
     6181 gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct gtcaatagat
     6241 ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa agtatttcga
     6301 tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat gctgtcaccc
     6361 accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga gtatctgtgt
     6421 cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc attccccata
     6481 acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata cctccgcgtt
     6541 cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc agtacttctg
     6601 caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc ccgactgaaa
     6661 aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa cggtccttct ttttctggat
     6721 aagcatagag ggctggtact gtttccaatt catttaagta caattgatag tattcaagca
     6781 taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt ctgctagatg
     6841 gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac cagttcatcc
     6901 atattttctg attgatttga ttggaatttc tcaatactga tttctttagt attttctaat
     6961 agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac cttacccgca
     7021 aagccaatat aaaatccttg tctgctcatt tcctctattc ctgtcatcat agcaggaaca
     7081 ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat ttcttgtatg
     7141 aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct tgccgtgata
     7201 ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata agcctttttt
     7261 tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt cagtataaat
     7321 ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac gatcccgcct
     7381 cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat acaatttgaa
     7441 atggtaatat tacgaatact accactcatt tcacttccta atacgacacc gccatgtcca
     7501 tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc gtacgtatct
     7561 tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca attactaata
     7621 cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc
     7681 aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt
     7741 ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg gaaactcatc
     7801 aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg ccaccatttt
     7861 tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac attttgagca
     7921 tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg aacaacagga
     7981 taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg aagttcgaca
     8041 ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac tacggtcccg
     8101 cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt caattcatca
     8161 attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact tcaatcaatg
     8221 cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca ctgatgtagt
     8281 aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga cagtttttat
     8341 tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat ccttgatgtg
     8401 atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt attcctttag
     8461 ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct ttccgttttg
     8521 tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga agtaatgcag
     8581 ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca tgaagtaatt
     8641 cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa aaatgatggg
     8701 atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat gcatgaatca
     8761 gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt ctcaaaacat
     8821 cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa ggagaaccca
     8881 tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa aaagctcctt
     8941 cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct tttttatatt
     9001 tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt acatggtcga
     9061 tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg aaatagtcga
     9121 tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa agtaattgga
     9181 accctttaag tacgacgcca tagtcataag accattttcc tttataccac ttttcttcat
     9241 ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag ttttccatta
     9301 tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag tatttgtccc
     9361 aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg tgtgattcga
     9421 tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc gtcaagctgt
     9481 cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga tcctacttca
     9541 ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc ttcattttct
     9601 ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga gaacgtcact
     9661 tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc catatcgata
     9721 aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc gccagtcatc
     9781 aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt cttcaattgt
     9841 gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc atttccttct
     9901 gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg cactaattcc
     9961 atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac cattccagtg
    10021 tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg ggtaactgca
    10081 aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa agctttcata
    10141 tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc ccagccattt
    10201 ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc actaggcgca
    10261 cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt ttctgtaaat
    10321 tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc tagattatcc
    10381 aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat cggtacgccg
    10441 tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg acttaaattt
    10501 ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa cgcattgcgg
    10561 acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag ttcatcagaa
    10621 gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt gaatgtcagt
    10681 tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga
    10741 tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga agaactgtta
    10801 cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag acctaatttt
    10861 acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat ttttgttttc
    10921 ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag aatttgctgt
    10981 ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac ctggcattgt
    11041 ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg agtaatgaag
    11101 atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt tccaatcata
    11161 atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg cagcaatata
    11221 caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta aaacaatccc
    11281 gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt taaaatatgt
    11341 gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc taatcactgc
    11401 aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga tgactaaacc
    11461 gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc cgtttatttt
    11521 atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc cgtcttctcc
    11581 tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga cccctttaaa
    11641 caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg tataaatata
    11701 ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt acatatgctc
    11761 gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga ttactggacg
    11821 aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat ccatttctgc
    11881 agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga tcgtagacca
    11941 gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt caggggatgt
    12001 tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata cccctccgtc
    12061 aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga agtgagggat
    12121 gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa
    12181 agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca agatcaacgt
    12241 atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc
    12301 gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag caataacgag
    12361 tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca gaatcatcac
    12421 atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc tgagcttttc
    12481 ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt ccataagccc
    12541 ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact atctgaatta
    12601 cgtatataat catttttacc gtaagtaaaa acgcttacat agcaatgatt tttgtgctat
    12661 acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc ttctattttt
    12721 ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc gatgataaat
    12781 tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc tatatgaaaa
    12841 agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt ctaattttgc
    12901 tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt gcttctacac
    12961 aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta gacgcgtatc
    13021 gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt ggtgttacag
    13081 gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt ctttccagaa
    13141 agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg ttgttcagcg
    13201 gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat tcttactggg
    13261 ccatgatcct gcctgtggct gttaatgcaa tgaatatgat cattatgaga aactttttcc
    13321 aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca gattttggtg
    13381 tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc tcgctttttt
    13441 atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac gattcatcta
    13501 aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag
    13561 aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct gttatcgtta
    13621 ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt gtcaaaggag
    13681 cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta gttgtgtcag
    13741 aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta aatttttgtg
    13801 aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca gaggttgtga
    13861 aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga cgagtaaccc
    13921 acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa aattatgcct
    13981 tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact gtgacaagac
    14041 ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat aaatacaaat
    14101 actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa acagcctctt
    14161 gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct ttttaaagaa
    14221 acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt ttacggagag
    14281 gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa caaaatcacc
    14341 aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt tataatgcaa
    14401 cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga cgatatgcat
    14461 aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc attctgtgat
    14521 tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat aattgttcag
    14581 tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc ccaataactg
    14641 tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac tcaaaggatt
    14701 tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca cttcctgatt
    14761 tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc taataagtgt
    14821 tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg gaccagtatt
    14881 tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt cattcagcgt
    14941 ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt ttacacgttt
    15001 ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat ctttatccat
    15061 tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc gattttcctt
    15121 tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac gctccccacc
    15181 gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt cattttgaga
    15241 actataacca aattgctgca accgctgatc tagttctcgc ctttctactc gatttctaaa
    15301 taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt gtgcacttaa
    15361 cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta gttctttccc
    15421 actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa
    15481 tctgtccgaa aataagtagg aaagaagacc aatgagtcct accagtgtta aagacactgc
    15541 aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt caacttctaa
    15601 tctgtaaatc caattattat aatctgattt cagtactata tacgttttat ctttttctct
    15661 tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg gttgctcttt
    15721 attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc
    15781 ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag caattcccaa
    15841 tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc ctttgttttt
    15901 cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact tttcaatttc
    15961 ctcttcggtc agttgactca acgatccatt gattactccc catttaccag ccaaactgac
    16021 taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt ctttcctgat
    16081 ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt
    16141 tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt
    16201 agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt cgatcgattc
    16261 ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc ctaataaaaa
    16321 catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc tctcctcctt
    16381 ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta aatgagcatt
    16441 ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt aacgtgtgca
    16501 atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga tgaatgcgtt
    16561 aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa
    16621 gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag tttaataaaa
    16681 aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc gccaaactat
    16741 agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg
    16801 cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca ttcagtatta
    16861 gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac agaaatcaac
    16921 ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca gcctttcatc
    16981 gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat caatgatcca
    17041 gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt agattacggt
    17101 attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt tcgttcgaca
    17161 aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca
    17221 gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat ggaataccta
    17281 ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat agcgattggt
    17341 gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc tctgatcggg
    17401 ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt acgtaagcct
    17461 ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga aggcaggacg
    17521 atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc gatcaattac
    17581 ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac agaaaggaag
    17641 tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc
    17701 ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt cagcagctga
    17761 ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa caaggtcttg
    17821 gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga ctcagcaatg
    17881 gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc aatccttatg
    17941 atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat atcgtctcca
    18001 atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat actcccccac
    18061 agagcctccc gggcaaactg acagcctttt tataccgacg tttcaaatta aacaaagctg
    18121 gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta aaagaaatca
    18181 ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg ttagaaaaag
    18241 aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct cgcgaagatg
    18301 caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa gcagaaccat
    18361 tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca ttcaaacagg
    18421 ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt aaggtgcatt
    18481 tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg
    18541 tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta ttcaatctag
    18601 aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct gaccaaatca
    18661 aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca ttaaatagtg
    18721 tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc
    18781 aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat cgaggcgatc
    18841 aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct tggtctgata
    18901 atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa gatttgtctc
    18961 aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat caaaaaggtg
    19021 tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca
    19081 acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat tccaaaacaa
    19141 acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc gcaaggtcat
    19201 aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg ctatccaatc
    19261 ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa tgtaaaaacc
    19321 aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag tcattacctt
    19381 tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat
    19441 gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa
    19501 cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat tttaaagcat
    19561 ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat tttagcggac
    19621 gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg tgagaacaat
    19681 acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt gaaatttcta
    19741 gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga agagctttta
    19801 acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg
    19861 aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact tggtgcattt
    19921 tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc agaaatgttt
    19981 ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt
    20041 gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt ttacgaaaac
    20101 ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc tttaggctgc
    20161 acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga
    20221 aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc ctctgctctt
    20281 gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc gtttggctca
    20341 tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa aggtcattgg
    20401 atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt agaatcgttt
    20461 gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca aaatgacgtt
    20521 cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa cagtataaaa
    20581 agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac gattgggcga
    20641 aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac tgtcacttag
    20701 atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct tggttaggag
    20761 gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag
    20821 gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca aaaattatcg
    20881 gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt attgacttgt
    20941 tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt ttggcagctc
    21001 ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag
    21061 atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa ccagatttga
    21121 aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa ggatttaatg
    21181 catgggtcac actactggaa gaagcagctg accaaacgat caccacctat caagaactga
    21241 tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt gtttccgacc
    21301 acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa gagatttttc
    21361 aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat cgtacagaaa
    21421 cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag ctgcacatcc
    21481 atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat accggctatg
    21541 atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac cgtgcagaag
    21601 aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac tatcctgcga
    21661 ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag ttaggttctg
    21721 gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa tttgctgatg
    21781 gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc ctgtcctaca
    21841 ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag
    21901 gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt agttatacaa
    21961 atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt aacgaatcaa
    22021 aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga agacggacca
    22081 aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc tttatgaaag
    22141 aagagataac atgcctacta tccatattgc cggtgattct actgcggcag aaaaagatga
    22201 aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt cacccgagat
    22261 ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc aggaaggaag
    22321 gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac aatttggaca
    22381 taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct atttaaaaaa
    22441 tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc
    22501 tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac ttggagacta
    22561 tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag atgtgttctc
    22621 aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat tctacctgca
    22681 tcttatacct gatacttgta aaaattaccc agaaggacta aaagacaata cccactttag
    22741 tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag aatcccgtct
    22801 gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc
    22861 gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc
    22921 acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt aaattaactg
    22981 acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac aatatcgaac
    23041 tttccatttt aggatgctat atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag
    23101 cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc aagatgattg
    23161 ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact gaagaagctt
    23221 atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat tttggcgtaa
    23281 ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta gctaaacgtt
    23341 taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg aatttgatgc
    23401 ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac aatttacatg
    23461 gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag atccgcatgg
    23521 tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc aaatatgagc
    23581 gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct gaagcaatag
    23641 aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga acgaatacag
    23701 aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct ttatcaggga
    23761 aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct tccatcttgg
    23821 ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga ttcttctcag
    23881 ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca aaatatattg
    23941 acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga agctggacta
    24001 agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa agaattgcaa
    24061 tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac gttgtcctct
    24121 caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt aggtatcacg
    24181 ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct cgccgcattc
    24241 gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag gcaagaaatg
    24301 gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc tcgagctcat
    24361 gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat ctatgaaaaa
    24421 ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc tagttttcag
    24481 atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg gcgcatccaa
    24541 cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc cgtttctgga
    24601 atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc tattggcgaa
    24661 ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca acatttggat
    24721 ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa accgattatc
    24781 gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg gccatctggc
    24841 tctggaaaat ctacactcct taacttattg attggattta tccagccgac ttcaggaaaa
    24901 atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg aaattattta
    24961 gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa catcacttat
    25021 ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc gcaactttct
    25081 tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca tggcaacaag
    25141 ctgtctggtg gccaaaagca acgtatctcc attgcacgag cgttgatacg tcagccaaaa
    25201 atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa aaaaatccag
    25261 caagccctga attacctgac agaaacaccc actactttta ttgtggctca tcggctttca
    25321 acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt
    25381 acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa
    25441 aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa acggtgttct
    25501 cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc tgttttttga
    25561 aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt aaatggtgtt
    25621 caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc cgaacgtctc
    25681 tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc gcgaactggt
    25741 gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc tttttgcatc
    25801 aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc tttcattgca
    25861 tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat ttgcatcgct
    25921 tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc gtacgcttct
    25981 ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt cgcgctatcg
    26041 tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat tgtaacatgg
    26101 ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt ctttagccgc
    26161 ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca gcccttcttt
    26221 tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc cattagaatt
    26281 tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat aggcatctgc
    26341 tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg atttgaagac
    26401 tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg attcttcgta
    26461 acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc
    26521 ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg tataacctgc
    26581 aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc tggtcatttc
    26641 atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat cgacgatcac
    26701 cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct gaccatcatt
    26761 ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt ccagtccacc
    26821 aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt cttctgcttt
    26881 atcatatcct ttttgtaaac cgatctcatg caaaagccat acagtaggca aattcaaact
    26941 ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa aattatgcgc
    27001 atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc cagcttccag
    27061 tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac gcatctgtgt
    27121 agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg cttgtacacc
    27181 acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat ctgaagcatt
    27241 tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat aatcctgatc
    27301 taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa gaccatactc
    27361 gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac cagaatctga
    27421 attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg cttgttcttg
    27481 ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt tccgatcatt
    27541 cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca ttcctgccaa
    27601 agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact ttctggaagc
    27661 gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca tggtcaagat
    27721 ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct tcgcttttcg
    27781 atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag tgatcgtact
    27841 tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac gggcaatccc
    27901 tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga ttactgcatc
    27961 ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg cgtacagtgt
    28021 gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc ctgattttaa
    28081 cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca ctaagacaca
    28141 gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga tgcgctttcg
    28201 ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga tcaaagcagc
    28261 ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc tatttgattc
    28321 aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat
    28381 atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa atgtaagttt
    28441 tttcttaaat atgttttaaa aatggtacaa ttagtctgcg attgcactga aaggagtaca
    28501 cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat tcgcgacttg
    28561 cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat acgtaaaaac
    28621 gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa tgaccagatc
    28681 acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg agatgcgaag
    28741 cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc tgcaggaatg
    28801 aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt agctgcttat
    28861 ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga aacaagcggt
    28921 gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat gttggaacaa
    28981 aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa agatatgacc
    29041 atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt agatgataaa
    29101 aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga acaaacacaa
    29161 gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt agctcataag
    29221 ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg actgatgctc
    29281 catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt cggtaaagct
    29341 ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca gataatgata
    29401 agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt tctgctaaca
    29461 gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata tattcactta
    29521 cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg attttttgaa
    29581 ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt gctaacgtca
    29641 acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa acaggatgat
    29701 cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt cctgctgcta
    29761 atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca tctggatggc
    29821 gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt tcttcattga
    29881 tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta ttttttcctt
    29941 gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt gtccaagcag
    30001 caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc cacccttttt
    30061 tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca atcgggccga
    30121 atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat acctgatcca
    30181 atgtttcttc tggtgttgtg gtagggatac ttaatttt
//
LOCUS       DASJTM010000029        30186 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJTM010000029 DASJTM010000000
VERSION     DASJTM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.029"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(162..1514)
                     /locus_tag="U4B29_001203"
                     /pseudo
     CDS             complement(162..1514)
                     /locus_tag="U4B29_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1511..2443)
                     /locus_tag="U4B29_001204"
     CDS             complement(1511..2443)
                     /locus_tag="U4B29_001204"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN2106164.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2440..3216)
                     /locus_tag="U4B29_001205"
     CDS             complement(2440..3216)
                     /locus_tag="U4B29_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN2106165.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3270..6455)
                     /gene="carB"
                     /locus_tag="U4B29_001206"
     CDS             complement(3270..6455)
                     /gene="carB"
                     /locus_tag="U4B29_001206"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN2106166.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6460..7536)
                     /gene="carA"
                     /locus_tag="U4B29_001207"
     CDS             complement(6460..7536)
                     /gene="carA"
                     /locus_tag="U4B29_001207"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN2106167.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7541..8821)
                     /locus_tag="U4B29_001208"
     CDS             complement(7541..8821)
                     /locus_tag="U4B29_001208"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN2106168.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8840..9766)
                     /locus_tag="U4B29_001209"
     CDS             complement(8840..9766)
                     /locus_tag="U4B29_001209"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN2106169.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9779..11062)
                     /locus_tag="U4B29_001210"
     CDS             complement(9779..11062)
                     /locus_tag="U4B29_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN2106170.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11076..11615)
                     /gene="pyrR"
                     /locus_tag="U4B29_001211"
     CDS             complement(11076..11615)
                     /gene="pyrR"
                     /locus_tag="U4B29_001211"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN2106171.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11850..12761)
                     /locus_tag="U4B29_001212"
     CDS             complement(11850..12761)
                     /locus_tag="U4B29_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2106172.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12758..13222)
                     /locus_tag="U4B29_001213"
     CDS             complement(12758..13222)
                     /locus_tag="U4B29_001213"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN2106173.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13227..13925)
                     /locus_tag="U4B29_001214"
     CDS             complement(13227..13925)
                     /locus_tag="U4B29_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2106174.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13912..14298)
                     /locus_tag="U4B29_001215"
     CDS             complement(13912..14298)
                     /locus_tag="U4B29_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106175.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14445..16115)
                     /locus_tag="U4B29_001216"
     CDS             complement(14445..16115)
                     /locus_tag="U4B29_001216"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN2106176.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16298..16531
                     /locus_tag="U4B29_001217"
     CDS             16298..16531
                     /locus_tag="U4B29_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN2106177.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16630..17061)
                     /locus_tag="U4B29_001218"
     CDS             complement(16630..17061)
                     /locus_tag="U4B29_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN2106178.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17140..17553
                     /locus_tag="U4B29_001219"
     CDS             17140..17553
                     /locus_tag="U4B29_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN2106179.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17591..18739)
                     /locus_tag="U4B29_001220"
     CDS             complement(17591..18739)
                     /locus_tag="U4B29_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2106180.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18861..19457)
                     /locus_tag="U4B29_001221"
     CDS             complement(18861..19457)
                     /locus_tag="U4B29_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2106181.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19657..22299)
                     /gene="alaS"
                     /locus_tag="U4B29_001222"
     CDS             complement(19657..22299)
                     /gene="alaS"
                     /locus_tag="U4B29_001222"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN2106182.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22687..23118
                     /locus_tag="U4B29_001223"
     CDS             22687..23118
                     /locus_tag="U4B29_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106183.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23163..24512)
                     /locus_tag="U4B29_001224"
     CDS             complement(23163..24512)
                     /locus_tag="U4B29_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2106184.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24582..25745)
                     /locus_tag="U4B29_001225"
     CDS             complement(24582..25745)
                     /locus_tag="U4B29_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2106185.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25874..26197
                     /locus_tag="U4B29_001226"
     CDS             25874..26197
                     /locus_tag="U4B29_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106186.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26380..27729)
                     /locus_tag="U4B29_001227"
     CDS             complement(26380..27729)
                     /locus_tag="U4B29_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106187.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27798..28751)
                     /locus_tag="U4B29_001228"
     CDS             complement(27798..28751)
                     /locus_tag="U4B29_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN2106188.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28853..30043)
                     /locus_tag="U4B29_001229"
     CDS             complement(28853..30043)
                     /locus_tag="U4B29_001229"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN2106189.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8467 a         6636 c         5317 g         9766 t
ORIGIN      
        1 tagaaaaatg aattttaaaa aaatcaaaaa ctgatcacga tagaagtttc ttttcatacc
       61 tgatgaaaag atgcttctcg caatcagttt ttggattttt gctattccaa ttgatgattc
      121 tggagacaca aatcggattt ctctatcttc atcaagcgcg cttaaatctg ccaattttca
      181 ggatcttttt tccattcttt tagcagagtt aattcttgct catcgatata acgatcttct
      241 aaagctgctt caatcaatac agaataattg gtcaacgtca taagagggat ttcggctttt
      301 tcgaaatttg cttttccttt tggcaactca tacgtaaaga tagccgcaac accaaggata
      361 tccgcacctt ctctttttgc tgcttcagct gcttccaaga cacttccgcc tgtagaaatc
      421 aaatcctcga tcacaaccat cttctgacct tcaaatatac gtccttcgat ttggttacct
      481 ttcccatgat ctttcgcctt acttcgaata tagaccattg gcaaatccag tatttccgct
      541 acccatgcgg catgagggat tcctgctgtt gcagttccag caatcacttg tacttcaggg
      601 taagcttctt tgattttgct tgctaatcct tttgcgatct cttttcggac ttttggatag
      661 ctcatcgtga ttcggttatc acagtaaatt gggcttttga ttccacttgc ccatgtaaaa
      721 ggattacttg gatttaaaaa tactgcttca atttctaata agtcttttgc gatactttgt
      781 tcaacattca ttttaggtta gctccattct gtttgaatcg tttgatatgc tttataagga
      841 ttagttgact gagtgattgg tcgacctata acgataaaag ttgagccgat cttttttgcc
      901 tgagaaggtg tgactactct tgtctgatct cctgcgactg ttccttctgg tcttataccc
      961 ggagttaagc aaacaaattc gtctcctgcc atttctttga tcgcttctac ttcccaagca
     1021 gaagaaacca caccatctaa tcctgctttt ttggcacagc tagcataatg gatcacactt
     1081 ttttctaaag aaacttcgat tagttgatcg gcatgcattt cttcttcact tgtagaagta
     1141 agctgagtga tagctagaag tagcggacgt tttccacctt ctggagtccc ctcttctaat
     1201 cctcgcattg ccgcctccat catacgaata ccgccagcgg catgaacaca ggtgagatca
     1261 acaccaagct ttgccaatcc ccgcattgct ttttcgaccg tattcggaat atcgtgaagt
     1321 ttcagatcta aaaatacatt atgaccagcc tcttttagat aacgtacgat ttcaggtcct
     1381 tcttggtaga acaattccat tccgattttc acaaacaatt tttcttcttt cgggaaaaat
     1441 cgaagaaagt cttctacctc ttgcctagtt gaaaaatcta atgcaataat tggtcgttgg
     1501 tctagttgat tcatcgttca cgttcctctc tcacttcttt gattaatctt tccagcgatt
     1561 cgatgcctag ttcgctcatt cgcacaggga gctctttaat tagtttagga cagatatacg
     1621 gatcggtgaa attcgcggtc cctatagcta cagcacttgc tccagccata aacatctcaa
     1681 gaacatcatc cactgtctgc acgccgccca tcccaatgat tggaaggctg gatatacttg
     1741 ctacttggtg aatcaaacga atagcgacag gcttgattgc tggtccggag agtccacctg
     1801 tttgattggc taaaatcggt tttcttgtct taaggtcaat ccgcatacca agaagagtat
     1861 tgatcatcgt aaacccatct gcccctccag cttcgattgc ctgagcgatt ggaacgatgt
     1921 ctgttacatt tggggacaat ttgacataga caggaacaga agaaactttc tttactgctt
     1981 gtgttaattg aaaagcaaca tcagaatctg ttccaaaagc aattccgcca tgtttgacat
     2041 ttgggcaaga aatgttcagc tcgattgctt tcacatttgg tgcttcgcca attttgctac
     2101 aaacttctac atagtcttct tcacaagcac cagcaacatt tgcgatgatg ggtaactcat
     2161 tgtactcctc taattctgga agaagtttat gcataacaac gtctagacca ggattttgca
     2221 aaccaattgc attcaacatg ccagatggtg tttccgccac tcttggcgtg ggattgccaa
     2281 agcgggcatt tggagtcgtt gccttgacca tgatcgatcc aagctgattc aaatcatagt
     2341 attttccgta ttctttgcca aaaccgaaac agccgcttgc cggcatgata ggatttttta
     2401 attctaggcc aggaagtttg accgctaaag gattctcatt catataatta cctctcctat
     2461 tttaaaaatc ggtccttcgt cacaaacttt cacacttttt gttcctgttt catcttccgg
     2521 cgtatgacaa acgcaagcat agcaagcccc tatcccacat gccatgcggg attctaatga
     2581 taactggaca ttttgatgcg aagaaaacag ctgttccact gtcttcaata acccgtttga
     2641 accacatgca aaaacagctg taggttccgt cttcgttcct aacaataaat taccgacatt
     2701 tccgtgcata ccgtatgttc catcgtcagt agcgattctt gtttctccta gacgattaaa
     2761 ttctttttca taataaacga cttcatgtgt ggcaaaacct aaaaaatgcg ttggcttaat
     2821 tcctttttcg actaactgtt ttgataactc atatagagga gggacaccga ttcctccacc
     2881 gattataaat acttcgtctc ctgtatgaag attggacaaa tcaaaaccat tgcctaaagg
     2941 acccatgaca tctaaatggt ctccggcaga aagagtagag aagactctag tcccctcacc
     3001 ttccacccga taaatgatac ggcaagtatt tgtttcgtgg tcgatatgat tcaagctaat
     3061 cggtcggcgt aatagcaagt ctgcacgcgg cacgagaata tgcaagaact gtcctggttg
     3121 ctgcatttct tttactagat cacctttcaa tgtcatttca aaaatctttg gtgccagctg
     3181 tttttgctga acgatttcca tcatttcttg tttcatctgt cccctcctgc aacggttttt
     3241 cgtctttttg tcttaacatg tgctcgccgt taaatagggt tagttgaaaa agcacgggat
     3301 tccatcactc gaacgatcgc atctgctgtg tctaaagaag taaacaatgg aataccatgt
     3361 tcaactgctt ctcttcggat gatgaaaccg tctttagaag catttccccg atctttatca
     3421 atcgtattga caaccacttg tgttttacca tctcttatta agtcgactac atttttttct
     3481 gctgcttcct cagaaatttt cgctactgtt tcaacgacca gaccattttt ttcgaaataa
     3541 gcagctgtat gtttcgttcc tatcagacta tagccaattt ctgtaaaacg tttagctaat
     3601 tctagtgctt cttcttttgt ttcgtccgca atcgtaaata atacggctcc atattcaggc
     3661 aagtgtaatc cagaagcttc gaatgccttg tacaaagctt tgtctaattg tctgtcagat
     3721 cccattactt ctccagtcga tttcatttca ggccctaagt aagtatcaac tttctgcaat
     3781 ttcgtaaaag aaaagacagg tgctttaaca tgaacttctt ttgtttctgg ataaagtccg
     3841 ttttcataac caagtgtctt tagtttctct cccaaaatag ctttcgtcgc aatttgtgcc
     3901 attggaattc ctgtcacctt acttaagaat ggaacagtcc gacttgctcg aggattgact
     3961 tcgatcacat aaacctcatt ttcatggatg acaaactgga tattcatcat cccgacacaa
     4021 ttcaaaccta atgctaagcg gatcgtgtaa tcttcgatcg ttttcttgat cgattcagat
     4081 aatgtttgag gtggatacac agccattgaa tcccctgaat ggacaccagc acgctcaata
     4141 tgctccatga tccctgggat gagtactgtt tcaccgtcac aaatcgcatc cacttcgcat
     4201 tcaccaccaa gcaagtaacg gtccactagt actggatgtt ctggagatgc tttcacagca
     4261 tgttccatgt aatcttctaa atctcgctgg ttttcaacga tttccatcgc acgtccgcct
     4321 aatacatagc ttggacgaac aagaactgga taaccaattc tcgaagcgat aaccacggct
     4381 tcttctttac ttgtagcagt atcccctaaa ggttgaggaa tatccaactc tttcagcgct
     4441 tgctcgaata aatcacgatt ttccgcacga tccaagtctt cgatcgttgt ccctaagatt
     4501 tttacaccag cagcagtcaa aggttcggct aagttgattg ctgtctgacc accaaattga
     4561 acgattactc ctatcggctg ttctagctcg atcacattca tcacatcttc gaaggtcaat
     4621 ggctcaaaat ataatttatc cgaaatagaa aagtcagtcg aaactgtttc aggattgctg
     4681 ttcatgatga ttgcttcgta tcctgcctgc tggattgctt tgacagaatg cacagtcgca
     4741 tagtcaaatt caaccccttg tccgatacgg ataggaccag atcccagtac tagaattgag
     4801 ggcttttctg atcgtgaact ttcattttca tattcatagg aactataaaa ataaggtgtt
     4861 tttgattcga attccgctgc acaagtgtca accatcttat atacaggaat caaatcattt
     4921 tcttttctca tctggcgaac ggcttcttcg tttatttccc acagctccgc cacttttttg
     4981 tctgtaaaac cgttttgttt cgctgttttc aatatcttgt catccaacgg ctgctgtttc
     5041 aactgctgtt cgatttctac gatatgcaac agtttatcta aaaagaataa atcgattttt
     5101 gtcaaatccg ctaattcttc tatggtgtac cctcttctaa tagcttcagc tacataaaac
     5161 aggcgatcat cttgtgctcg aaccactttt tggattaggt catcgtccgt gatgtctgcc
     5221 aactcaggaa gttcgatatg gtgtgtaccg atttctaaag aacgcacagc tttcaataaa
     5281 gactcttcga tattccgacc gatagccatc acttcaccag ttgctttcat ctgcgtgcct
     5341 aaaagccgtt ctccgttttc aaacttatca aacggccagc gaggaatttt agctacaaca
     5401 taatctaatg caggttcaaa ttctgcaaaa gtcgttcctg taacaggatt tttcatttcg
     5461 tctaatgtta atccaagcgc tattttagca gctagcttag cgatcggata gccagtcgcc
     5521 ttacttgcta acgcagatga acgagaaaca cgcggattga cttcaatcac ataataatta
     5581 aaactatgtg gatcaagagc gagttggaca ttacagcctc cctcgatctt aagtgctcga
     5641 ataatcgaaa gagaagcatc acgaagcatt tgatattcat ggtcagacaa tgtttgacta
     5701 ggggcaaata cgatagaatc acctgtatgg atacccaccg ggtcaaaatt ttccatattg
     5761 cagacaacga ttgcattatc agccgaatct ctcatcactt catattcaat ttctttaaaa
     5821 cctgcaatac ttcgttcaat caaacattgg gtagcggggg aaagacttaa tccattttct
     5881 gatacgatac gtaattcttc ttcattttca cacatacctc caccagttcc gcctaaagta
     5941 aaggctggac gaacgatgac aggatagccg attttatttg caaaatctac agcttcttct
     6001 actgtattga cgatttcaga ctcggggatt ggctgattca attctttcat caactgtttg
     6061 aatagatcac gatcttctgc ttgatcgatc gcagaaagct ttgttcctag aagttctatg
     6121 ttcaattctt ctaaaatacc tgatgctgcc agctccattg ccatattgag gcctgtctgt
     6181 ccgcctaaag ttggaaggag agcatctgga cgctcttttc gaagaatcct tgatacaaat
     6241 tcaagtgtga tcggttcgat atatacttga tctgccactt ctttatccgt catgatcgtc
     6301 gcaggattag aatttactaa aaccacttca tatccttctt cttttaaggc aaggcaggct
     6361 tgtgttcctg cgtagtcaaa ttctgcggct tgaccaatga tgatcggacc agatccaatc
     6421 accataattt tcttgatatc tgtacgtttc ggcatctgtt tactgctcct ttcctgcgtc
     6481 catcatttcc ataaattcat caaataaatg tagtgcatca tgcggaccag gggcagcatc
     6541 tggatgaaac tgcacactga aagcgggata atctctgtgt ttgactcctt ctactgttcc
     6601 gtcatttact tctacatgag ttacgatgag tttttctgga tcaatcgtgg cttcatcgac
     6661 tgcatagcca tgattttgag aagtaaaatc aattcttccg gtcgctactt ctcgtaccgg
     6721 gtgatttaat cctcgatgtc cgaatttcat tttataggta tctgcaccgt tggctaaagc
     6781 aaataactgg tgaccgagac aaataccaaa aatcggtact tttccttgga tttttttgat
     6841 catttcgatt gcttctggca catcttttgg atcaccaggt ccattcgtca gcatgactcc
     6901 gtctggtgat agcgctaaaa tcgtctcggc atctgtgtta tagggaagta ctgtgaggtt
     6961 acagtttctt ttactcaatt ctcgtaaaat actatgtttc aagccgaagt caatgacaat
     7021 gactttccga cctgttcctg gacttggata aggtttgtta gttgagactt gctgcacttg
     7081 attggttgct agaacggttg ctttcagctg atcaaatgca tgttcgaagg aatctccagc
     7141 atcaactata ctagctttca tcgttccgac ttcacggatt ttgcgagtaa gggcgcgcgt
     7201 atcgatacct gatattccag gaattccttt ttgtttcaaa aatgaatcta gcgtcatttg
     7261 gtttcgccag tttgaagcta cacgtgcatg ttcttttact actactcctt tgcatgttgg
     7321 agaaatcgat tcataatcat ctctatttac cccataatta cccaccatag gataggtaaa
     7381 tgtaatgatt tgaccgttaa aactttgatc agtgatagtt tcttgataac cagtcatact
     7441 tgttgtaaaa acgatttctc cgaagacact agcatctgca ccaaaagcct ttccttcgaa
     7501 aactgttcca tcttctaata taagcaaacg ttccatactt ttattctccc ttctgccaca
     7561 caagttttcc gttgacaaaa gtcatttgtg tttcgccttt tactttccag ccgataaaag
     7621 gtgtattctc cccttttgat aaaaagtctt ctttatcgat cgtgcaagtt tgtgtaatat
     7681 caaaaactgc tacatcagcg ggggcaccaa ccgttagcgt tcctgcattc aaaccaaaaa
     7741 tctcagccgg tttaactgcc aaccaattga tcacttgttc caacgtgaat cgaccagttt
     7801 ctacaaaatg agtatagatc agttgaaacg ctgtttcgct tcctacgata ccaaatggtg
     7861 atttcatgaa actttggctt ttttcttcta atccatgggg ggcatggtct gtagcgatgc
     7921 aatcgatcgt accgtcaagc aagccttcga tcaaagcttc tctgtcctcc cgtcctctta
     7981 gcggcgggtt cattttccaa aagccgaaat cttcgggaat atcttcgtcg atcaaaataa
     8041 gatgatgggg tgaaacttct gctgttacgt ggattcctgc tttttttgcg tctctgatta
     8101 cacggacact ttctttagta gaaacatggc aaacatggta atgaacacca gttgcttcag
     8161 ctagcaacag atctctagca atttgagaag actcggttac gcttaagatc ccgggaagac
     8221 caagttcttc tgccttcttg ccagcgtgca taacgccgcc aaataataaa gattcatctt
     8281 ctgtatgggc tactagggct ttattgtttt ttgcagcttc tttcatagcc agatacatcg
     8341 tcccagctgt ttggacacct acaccgtcat ttgtgaaagc aaaagcacct tcttcaatca
     8401 gtgcttcttg atcaactaat ttctcacttc gcagattttc tgtgattggc gcatactgca
     8461 ataccttcac aactgcatct tttctaatga tgtcatatac ttgtctcagc ttttctgccg
     8521 tatcaggtac aggatttaaa ttcggcatcg cgcatacagt agtgaaaccg ccacgagcag
     8581 ctgagcgagt cccagcttcg atcgtctcct tgtatgtaaa tcctggctct ctcaaatgaa
     8641 catggacgtc cactaaacca ggtgtgatca attgtccctt tgcatcaaac acttcatcaa
     8701 attcggcttc tgagaatcct gtaccgattg ctttgattcg cccatcttct atccaaattt
     8761 ctgctggtgt cgtcatattt tttcgtgtat tgatctgacc gtttttaatg agtgttttca
     8821 ttttttcatc ctcctataat tacgcttttc catttagcac tgcttctaag attgccattc
     8881 tgacgaatac tccattgctc atttgctgaa cgatccgcga ctgtaatcct tccactaatg
     8941 aatcagctaa ttctacatca cggttaactg gtgctgggtg cataatgatt gcatgttttt
     9001 gcaacttctt cgcacgctca actgttaacc catgttcctg atggtagcct tcttttgaga
     9061 aactttcttt tccatcatgg cgttcgtgtt gcacccgaag catcatcatg acatcaactt
     9121 gatctagtag ttcatccaat ggcatataat gtccgtacac ttcgaattca tcgtcgtacc
     9181 attctctagg acctgagaag aatacttgtg cgcctagacg ttttaacatc tgcatattgg
     9241 atttagctac gcgagagtga gtgatatcac ctacgatggc aactttcaga ccttcaaaat
     9301 gaccaaattc ttcataaatg gtcatcaaat caagcaagca ttgggttgga tgctgtccgc
     9361 ttccgtctcc tccattgata atcgaacact ggattgtttt actttgtatc agttcgtcat
     9421 aatagttctc atcaccatga cggataaccg ctacgtctac gcctaatgct gacatcgtca
     9481 gtactgtatc gtatagtgtt tccccttttt ggacagagct tgtgcttgct tcaaattcaa
     9541 tcacttctag ccctagtttt ttctccgcta catcgaagct tttatgtgtg cgagtactat
     9601 tttcgaaaaa gaggtttgta gcaaagtact ggcgcttttg cggagtccaa ttagcacctt
     9661 gcttgaattc ctgccctctg cggatcaagc ccataacttc ttgatcagta agtgcctcaa
     9721 cggtcaataa atgctttaag ctgatacgtt ctgatttgat gatcataatg tttcctcctt
     9781 agttttcttc gcttcgtgaa gtttctggta agataagatt taaaatgatg ccaagtacag
     9841 ttgctaatgc cattgccgac aatgtaaatg tgcctacttc aaagacgagt ccgccaattc
     9901 ctactactaa gataactgaa gcaatcaaca gatttttctt tttatcaaaa tcgattttgt
     9961 tctcgattaa aatcttcatt ccacttgcgg caattactcc aaataagata aaactgattc
    10021 ctgagatgac tggtccaggg atacttaaaa tcaaagcgct taattttcct acgaatccta
    10081 aaatgactgc aaatactgca gctccaccga tcacaaacac gctatggaca cgtgtgatag
    10141 ccagaacacc aatgttttca ccgtaacttg ttacaggagg acctccgaca aaggctgcca
    10201 cgatttgtgc taatccatca cctgtcaacg tcttatgaag acccggttct tcaaagaaat
    10261 tacgtttagt tagtttattc aataccataa gatggccgat atgctctgtc atcgtcacaa
    10321 aagcgatggg tgccattgtt gtgattgctc caagatatag ctttggttca tattgtacaa
    10381 agggaatctc aaagttagga acagcaaacc aggcagcgtc tacgattggc ttagtatcca
    10441 cgattccaaa cagcaaggct acgatgtatc cgctaataat acctagtaaa atcggaatca
    10501 atccgagaaa acctcttagc cacatgttga agaaaattgt taatcctaat gtgatcagcg
    10561 ctacagcaat atacttgaaa tcatacgtgc cctgattata catagcattt gttgctgcat
    10621 ttgccgcaag tcccaacccg ataaccatga tgactggacc gacgacgatt ggaggcaaga
    10681 ttttatccag ccatgctgaa ccgatctttt tgatgatcag cgcaacaatc aaatagacta
    10741 atccagttgt gactgctcct tgtgcaatcg ctggataacc atctgttttc atcaaaagct
    10801 gcatcgctgc aatgaaagcg aagctgcttc ctaagtacgc aggtatcttt cctttagtag
    10861 taaataagta caccaatgta cctagtcctg aactgactag cgcaatacct ggatcgatac
    10921 ctactaagat ggggactaac acggttgctc cgaacatcgt gaacaagtgt tgtaaactta
    10981 atcctaccca atgtcctggg gttgggcgat cgtggatatc taacaccacg tcatcattat
    11041 gaaatccttt tttctccgac atgtttctct ctcctctaat cttcctcttt caaaatcatg
    11101 atgcgatctt gtccatcaag ctcttgcatc tctactaaaa tctcttccgc ccgagatgtt
    11161 ggaatgttct tcccaacata atctgcacgg attggcaatt ctcgatgccc acgatcgacc
    11221 aggactgcta atgaaatttt tcttggtctg ccaaaatcca ttactgcgtc cattgctgct
    11281 cgaatcgttc gtcctgtata caaaacatca tcaatcaaaa taacttcttt tccttctaag
    11341 gaaactggga tgtcagaaga atgaagttcc ggttcttctg gattttcttt tttgtcatct
    11401 cggtataaag tgatatccag ttctcctaca ggaatatcga tatcttctag ctgcttcagg
    11461 cgttcggcaa ttcttgatgc aatgtagatt cctcgtgttt tgatacctac caatacgata
    11521 tcttggatac tatggtttct ctcgataatc tcataagtaa ttcgagtcaa tgctcgcttc
    11581 atggttacct gatctactac ttcttttgct tgcattttca ttccccctac ttttttgcat
    11641 aaaaaaactc ctgccctaag aggacaggag gaatgtttag actgagctta tactgataca
    11701 ctgatagcta tactatgtca atgagcaatc tacttatggc gcaccatacg tatccttgct
    11761 cccacccttc ttagcctcac gggactaaac ttaaaggaat tctattaagt tttatgcttt
    11821 cgttgttaat tactataccg ttatctaaat taaaatgcaa tgttttttct taatttttct
    11881 aaagtttttt caaaaacttc tggaagaggt gcttcaaaga tcatcttttc gtttgtagtc
    11941 gggtgcgtaa aacctaacat ttgcgcatgg agaaactgac cattgccttt caaagttttc
    12001 ttcggtccgt atactggatc tccagctaac ggataaccga tatacttcat atgcacacgg
    12061 atctggtgtg ttcttccggt ttctagtttt aattcaacca gagtaaatcc ttcgaaacgt
    12121 tccaatacag taaaatgtgt gacagccgac ttcccatctt ctcttatcgc ctgcattttc
    12181 cggttttcct tcgaacggcc gattggcgca ttgatggttc ctttttcgtg aggaatctct
    12241 ccatggacta aagcaacata tttgcgcaaa gaggtcttat cttttaattg ctgagctaat
    12301 gattcgtgtg cctggtcatt tttggcaacc attagcagtc cggaagtatc tttgtcgatt
    12361 cggtgaacaa tacctggacg aataacgccg ttgatcgtag aaagatcctt gatatggtac
    12421 atcagcgcat tgaccattgt gccatgcgta tgacctgctg aaggatgaac gaccattcct
    12481 tgaggtttgt ttatcaccgc tacatcttca tcttcgtaaa cgatcgtcaa cgggatattt
    12541 tctgggacca catctaatgt ttctggttcg gggatcaaga cagtaatctc gtctccttgt
    12601 tttactttgt aatttgcttt gactggctgt ccattgacag aaaccgctcc atttttcagc
    12661 cacagctgaa tttgcgaacg actgtaatcg gtgaataaat tagtcaatac tttatcgatc
    12721 ctaccagatt catggtgaat cgtcgtttta agttctgtca tttttttctt tgatcctttt
    12781 catctaaaat aatataaatt aacatacaaa tcactccaca tacaagtgtc acatctgcta
    12841 cgttaaaaat cggaaagctc ataaaatctg tctggaacat atctactaca tagccaagac
    12901 gaatacggtc gataaaattg ccaatcgcac cagctagaat caaactcaat ccaatcatga
    12961 accagcgatt acctttttca cgatttttaa tcatcaatgt gagaatcaca acgactgcaa
    13021 tgagtgtcac tatagtaaag aaccacatct tcccttctag catactccat gcggctccag
    13081 tgtttcgaat atgattcagt gacaaaatac ctggtataag atttttcgtc tctccaagcg
    13141 ctagattatc agtgatccac catttgacca actgatccac tcctactagc aaagcgctga
    13201 atatccaata aataattaac aaaaaatcaa tccttttcta tttgtttata aatatcacga
    13261 ggtcgtacga tcccgagcag attaccatcg ggattcccat ctttagtgat aagtacggct
    13321 tccaaagtcc cttgctcacg aaacatcgct tcaacctcgt aaatatgtgt tttagctgga
    13381 acaaaatgat aatttgtgta ctttccgttt tggatgatca aatccaacgc tcgtttattt
    13441 gttaaatcaa ccggaccttt ttggctttct tttgctagcc aaaacccgat attacgtaaa
    13501 gtaatcaatg ctacaaattt tcctttattg tataacggaa attgtgaata ccttttttct
    13561 gctactatct ttaacagttc aagtaacgga agatcttgtt caaaacctgt tacacgtttt
    13621 gcaaatcgag gcagcacggt ttcaggctga atcagttctt gttcgatcag ctggattcgc
    13681 ttgactgtcc aatcatttgg ctctgcaatg acaaaatcga ctgcgatccg atcatggacg
    13741 atcgcattcc ttaactgagc aagctgtaaa agatcatctt cgtattttct gatcattaat
    13801 tgttttcttt gagctaggcg tctgacaagt tcggtaaatc ccatatttcg tgcatttccc
    13861 atctcgtctc tcatccattt ttcgattcga ttgaaactga ttaaaaaaag atcagagttt
    13921 gccataattt tcctccttga tcaaatatgc ccggtttttt atatagaaaa agaacgcaat
    13981 caacacaata actgcaaaaa aactgaaaaa taaattttgt aggaaaacgg cccccaaccc
    14041 agttgctaga aataaaaacg gatagatttg aagaatcaag taattcattg tgatcacata
    14101 agcaggcttt gcttctttca ctgtataaac ggggcttttt agccaataaa aggtgacctg
    14161 catcgtaaag ggcacttcca gatcaagcgt tatcttttgt tcatgactga gaacactgcg
    14221 tttttcattg ttgattctta gctgaatcga gctacccata ccgtaaaaac cggtttttct
    14281 tataaatgtc gttttcatca taccctccta ttatgcttta gtatagctca aaatacaata
    14341 aagaaaaaga atgatcctag tgtaaacaaa attaattccc tatcgtctaa aaaaagaagc
    14401 tgtagtcaaa atacagctcc ttcgatggta gattataatc aagcttaaaa caatcccaac
    14461 acttctccgt catttgtcac gtccatattc aatgcagcag gttttttagg caaaccaggc
    14521 atcgtcataa catctccagt caaggctact aaaaatcctg cccctaattt tgggatgatt
    14581 tcgcggatcg tgatggtgaa atcttccggt gcacctagca gtgaaggatc atcagaaaat
    14641 gaatactgtg tttttgccat acaaatcggc agtttgtccc atccattctt cgtaaactct
    14701 tctatttgtt tttgcgcttt tttgctgaaa acaacaccat ttcctccgta aattttcttg
    14761 acgattgttt ctgtcttttc ttgtatcgtt tcttctaggc ggtataatgg cttgtaatta
    14821 gcttcttttc tatcaatcaa tcgtactaca gcttttgcta aatcaacgcc gccttctgct
    14881 ccatctgccc atacactcgc tctcttggca agaatcccct gattttcaca caagtgctcc
    14941 aacagtgtga gttcattgtc tgtatctgtt acgaattcat tgattgcgac gatcacaggt
    15001 agctgatatt gttccatatt acgtatatgg cgctttaaat ttgcaaaacc tactttcaaa
    15061 gcatcaagat tctcattttt aagttcatct tttttcattc cgccatgcat tttcaatgca
    15121 cggacagtgg cgacaatcac cacagcatcc ggcgcttttt tcaagttagg tactttgata
    15181 tctaaaaatt tctctccgcc aagatctgca ccaaatcctg cctctgtgac aacgtaatct
    15241 gctaatctca atgctgtctt ggtagctaaa atactattgc agccatgagc gatattggca
    15301 aacggtcccc cgtggacaaa agctggtgtt ccgtaaattg tttgtacaag attcggcttg
    15361 attgcatctt tcagcagtaa tgccagagcg ccttctgctt tcaagtctcc agcagtcaca
    15421 ggctgacggt caaatgtata gccgacaacg atccttgcaa gacgagcctt taaatcttca
    15481 aggtctgccg caagacacaa gatagccatg atttcgctgg caactgtgat atcaaaccca
    15541 tcttctcttg gaacaccttg gattggccca cccaagccaa caattacttt acgcagttca
    15601 cgatcattga gatcgactac ccgtttccaa atcactcggc gggcatcgat gttcaattca
    15661 tttccttgat gaatatgatt atctaaaaga gcagataatg cattattagc tgttgtgata
    15721 gcatgcatgt caccagtaaa atgcaagttg atatcttcca taggcaatac ctgtgcatat
    15781 cctccgcctg cagcaccacc tttgattccc atcacaggac caagtgaggg ttctcgcaat
    15841 gcaataacag attttttatt gattttattg agcgcgtctc ctaaccctac agtgatcgtt
    15901 gactttcctt cgccagctgg tgttggattg atggaagtaa ctaagatcaa atgcccctca
    15961 gcgttatcct ttgatcgatt gattccagaa aaattgattt tcgccttata tttcccatac
    16021 agttccaaat cgtcttcctt aagatctaat ttactagcca cttcaacaat cggttctagc
    16081 tctgtttctt gtgaaatttg cagatcattc ttcatttgat tacccctttt cacgaacttt
    16141 attcaacaaa atatcttctt tgttaagata tgccaattat accttttttt attcagaaaa
    16201 aaaacataaa attacaggtt tttaatttat ttgacgtact tataaatact ctttataatg
    16261 aaggctatga tcagacttta gaaaggtcgt gagtgctttg accacaggaa ttgtaaaatg
    16321 gtttgacaac aaaaaaggat acggctttat cagttatgat gacacagaag aaatttttgt
    16381 acattttact gcgattgaag aagagggatt caaaactttg gaagaaaatc aagtggtcga
    16441 atttgagatc atcgaaggga atcgaggaac acaagctgcc catgtcaaga aaggcaacgt
    16501 tcagactacc gatactagta tgcatgaata gataaacaaa tagagtgaga cgctaaacgg
    16561 gatgaaccgt acgtctcact cttttctttt cctgtaaaaa ttcgtttttt taggttctga
    16621 tttttaaaat tagctttttc tttttttcgt ataaggataa ggaattgatt ctgatttggg
    16681 tccgtttttt tcaaaatgat caaaaaggac tacttgatct ttttttcgca tcatgaaaac
    16741 agatggggct ttctcatctt gcaacatcat ttgcacccca gtattagttg gcgtcaaaga
    16801 tcgaatctgt ccgacagata tttctcggtc tttccaaaaa gcagcataac gtataatcac
    16861 agagtttgat tttaaaataa aagaacgccg caaacctaga aatagaaaaa caagaaaaat
    16921 acctaaaaca aaattgcttt tccaataagg acctgtgttt tctaaagaaa gaatcagact
    16981 aaaaaacaga atgatcaagg tcaatgacca atagatgatc gtatgtgcca gttcaggctg
    17041 ccatcgaata ttttttttca ttctctaacc tctcaccgat tcagtaatga tataatctta
    17101 ccataacatt agtttgaaca aaaaggagaa tctcttttta tgttaaaagt atacattgat
    17161 gcctctacaa aaggcaaccc tggtcctagt ggaggtggaa tcttgattgt ctatcaaggt
    17221 aaacaagaac agctatctat cccgctttct atagggacaa accatcaagc agagttcgaa
    17281 gtctttttaa aaactttaga atttctaaag aaaaacgact tacagaatga aacgattttc
    17341 tgtttttcag acagcaaaac actagtttct acggtcgaca aaaacgcaac gaagaacgag
    17401 ctcttccttc cctaccttca aagaatccaa gaacttctat ccgaattttc tttattgatc
    17461 ctgcaatggt tgcccgaaaa caaaaataaa ggagcagata atcttgcacg tcaggctctc
    17521 cataaacaat taaaagaaaa aaagactctt taaattaaaa actttaagga gtctttttat
    17581 agtttctcat ttaatataaa agataattgt gatcaaacgt aatttccaat tggtaatctc
    17641 cgattttttc ttctatttga ctacgtaaga ttctttctag ttctgtatca acaaactgga
    17701 atttctcagg gaccactaca tcaaattgca gcgtttttcc ttgatgaatt actctaaaat
    17761 catgaatatt aagccctgta cctgtttcaa gaagaatttg ctttaactgc cgcaagatat
    17821 tatagtattt ttcgttatca acaggcattg gatccaagtg acagacaagt tccacatcca
    17881 aagacttttt aaagtctttt tctatcagat caataatctt atgagccaca ttcaaattca
    17941 ttgattcatc tacttcgatg tggactgatg caaagcgttt attgggtcca tagttatgca
    18001 ccaataaatc atgatagcca agtatatcat cgcaagcatt taaatgctct tccatttctt
    18061 caatctctgc tggcgttggg cgacttccta atagttcgta tacaaaattt cgaagcatcg
    18121 tcaatccact ataaaggata tataatgcta aagcaaaacc tatatatcca tcaatacgcc
    18181 atccagttac ccattcgatt gctgctgaaa gcaatacagc cagtgtagta aagacatcat
    18241 tataactatc tgttgctgta gctctcaatg tttcagaatc aatggattgc gcaataacct
    18301 gatacatttt gccttgtgca aatttcaaaa agatggaaat gattaggaca aaaaagacga
    18361 tgggtgttaa agagacattt tctggatgga tgattttttc agcagaagac tctaaaaatt
    18421 gaaatcctac ataagtaatg atgatcgaaa caaataaccc actgatatat tcgaagcgtt
    18481 cgtgtccata aggatgttcc tgatctgctg gctttgcagc aattttaaat ccgatcaaag
    18541 tcaatatgga tgaagcagta tctgatagac tgttcactgc atcagccata atggagacac
    18601 ttcctgaaaa aagcccgatc attagcttag agacaaacaa gatcagattc gtaaccaatc
    18661 ctaagatacc cgctagtact cctttttttg tccgctgttg cggtatttgt ttcttttttt
    18721 cttcttgttt cttagccaag ttttttaccc cttctagttt gcttgttgct caggtaaaaa
    18781 gatggagagg acgattgttt tcctctcctc tcttcctttt tctatctatc gtgcgtccta
    18841 ttactattaa aagatggtca ttattctggt ttcaacggtg ttgctgcaaa taacgctttt
    18901 gctagtaatg gtgttatgat tcccgcacct attgcttctg gtatcccatt gattgctgca
    18961 acccctgcta aggttttaaa taaaagagca gagtctacac cataggcatc tgccgttgca
    19021 cctgtataaa ataatcccat gaatcctaac actaagactg tattgaccaa tgaacccagt
    19081 acccctgcaa aggctgagct gatcgttatt gatttatttt tttgataaat ccagtggaaa
    19141 accaagcctg ctactaagcc aacaagtact ctgggaacta ccgagataat cggattcgtg
    19201 aacactaatg tatcaaatgg cgttgttggc atggcatagg cacgaacaat tgtagcgatt
    19261 ccccaaaaca agccaatcac catgccatct tttgggccta atactactgc agcaataatt
    19321 actgtgatat ggataattgt taaactaatg aatcctaatg gaataaaccc cagccaagga
    19381 accatcgctt gtacgatgat gattgctaac aagatcgcac gtaagactag tcgaaaggtc
    19441 ttatttctac tattcatttt ttcacccctt tttatttata ctaatcgtaa aaatttctgt
    19501 cactttcgtc aactaaagac catccgaatc cgaccatttg attagatgcg ccatccaatt
    19561 gaaaaaagta tgagggcaag acttgtttgt cttcgccccc ataatcatgt ttcgttcaat
    19621 gtttgaactt ttgtttttct gtcaaacgta aacgccctat tgttgcgcaa cccattcttt
    19681 cgcagcttta agtgcatctg gaataccagc aggatttttc ccgccagctt gtgccatgtc
    19741 tggacgtcca ccaccgccac cgcctacttt cggtgcgatt gctttaatca agtcgccagc
    19801 ttttagtcca gcttcatttg attctttcgt cattgccgct agcagactga cttttccttc
    19861 ttgttcgttt gctaaaacaa gaatatctga aatatttttt tgtttccatt ggtcagctaa
    19921 ttgacgcagc tgattcatat ctttcacttt tacttgtgct gcaatgattg tttttccatt
    19981 gatcgtttct ggatctttga atacatcacc agcttgttga ttcgctaact tgctagctaa
    20041 ttgttcattt tccttttgta gctctcttac ttgctgttgc aattgttccg cttttgaaac
    20101 aacttctttc aattgaggtg atttaacaat tgttgctact tgttttagtt gtttttcttc
    20161 ttcttcaagt agttcgtacg cttctttact tgttacagcc tcgattcgtc gcacgcctgc
    20221 accaattcct gattcagaaa cgattttgaa gattccgatg ttttctgtat ttgctacatg
    20281 tgtgcctccg cacagttcga ttgaccagcc gccaatattt actactcgaa cgtcatgtcc
    20341 gtatttttca ccaaacaaag ccattgcacc catgtttttg gctgtatcga tatctgtttc
    20401 aattgtttca actgggatag cttcccagat tttttcgttg acgattcttt ccatttcagc
    20461 taattcatct gaagtgattt gaccaaagtg ggtaaagtcg aatcgtaaat gtccaggagc
    20521 gactaaagaa ccagcttgat ttgcatgctc gccaagtact tctttcaatg ccttgtgcag
    20581 tagatgggtt gctgtatggt ttttgatgat ccgattgcgc atcgattgat cgatcaatag
    20641 atgataggtt tgtccttcac tcattggtgc ttttacttct acggtgtgca taaattgacc
    20701 attaggcgct ttttgaacat ctgtaacaac tgctacgatt tcgcctactt cattcatgat
    20761 ccatccttga tccgctactt gcccacccat ttctgcatag aaaggtgttt gatcaaagat
    20821 catttgagct tcaccttctg cgacttcatt caaaagagac tcacctttaa cgatgaccaa
    20881 taattgacta tctgattctt gttctgtgta tccaatgaat ttactttcta ctttgatatc
    20941 tgtcaataaa gcagattgta cccccattga tttttcagta ctgcgggcag agcgagcacg
    21001 ttcgcgttgt gcatccattt ctttttcaaa accagcatga tcaactttca gtccttcatc
    21061 ttctgccacc tcttccgtta attctaccgg gaagccatat gtgtcgtata gtttgaagat
    21121 gtcttttcct gctagagtcg tcccgttttc gttttttact tcttggatca cttgatttaa
    21181 gatttccaaa ccttcattga ttgtttcatt gaaacgttct tcttctgtac ggataacttt
    21241 ttcaataaat tctttttgtt gaagtacttc aggataataa cttaccatga tttccccaac
    21301 aacagggact aaacgatata agaatgcttg ttcaatacct aattttttcc catgcatgac
    21361 agcacgacga agcaaacggc gtaagacata ccctctccct tcattcgatg gcaatgcacc
    21421 gtcgccaata gcaaatgaaa gggcgcgaat atgatctgcg attactttgt aagaaatatc
    21481 tgttactggg ttttcaccat attttacttg tttgcttagc ttttctacgg cgtggataat
    21541 aggcataaac aagtctgtct caaagttcgt tgacgcatct tgaacgattg agaccatgcg
    21601 ttccaatccc atccccgtat caatgttttt atgaggaagt ggttcaaatg tgttatcagg
    21661 cttatggttg aattctgaaa atactaagtt ccagatttct aaataacgtt cattttctcc
    21721 tcctggatag ttttcaggat cgtcttctgc taaatcgtta taagcttctc cacggtcata
    21781 gaaaatttcc gtatccggac cacatgggcc tgcaccgata tcccagaagt tgtcttctac
    21841 atcaataatg tgatctagtg gcaggccaac ttcttcatgc cagatgcgtt ttgcttctgt
    21901 atcttttgga taaactgtta cataaagttt ttctggatcg aatgccatcc attttggatc
    21961 agtcaaaaat tcccatgccc agtggatggc ttcatttttg aaatagtcac caatcgaaaa
    22021 attacccagc atctcaaaca tcgtatggtg ccgtgctgtt ttaccgacat tttcgatatc
    22081 atttgttcga atagattttt gggcatttgt gattcttgga ttttctggca caactgaacc
    22141 atcaaaatat tttttcaatg ttgcaacacc agagttgatc cacaataagg ttggatcgtt
    22201 cacggggatc aaagacgcac taggttcaat cgtatgtcct ttagattgga agaaatcaag
    22261 aaacatttgt cgaacttgtg cactacttaa ttgtttcatg tcattacctg ctttctataa
    22321 attaaataca aaaaaacacc atcacagcca aggacgaaaa ctcgcggtac caccttgatt
    22381 gcaatgatgt gtaatcatta cctcttaaac tcgataacgc tgagctgcgt cggtatttct
    22441 accaaaatag aaaaagggag caatttgatt agtgactatt cttttttcag ccaagaaaga
    22501 attttctgtg agtcttaaaa aacaaatcat atccttttcg tacttaatta gtatacgcat
    22561 tctattgatc actgtcaata tctcaatttt tattttcatt tccttttggt catactccac
    22621 gatttttggt acgataagaa taaaggaatc aatacatctc tgctattcat gacaaggagg
    22681 aatatgatga aaaaaatagt tggagcggat gaatggatac gcgcagctag ctgctttttg
    22741 agatatcaag tttttgtatt agaacaaggg attcctgcag aactagaatt cgacggaaaa
    22801 gatatcccgc aacaaaccta ttatctattt cttgatcaaa aaagaccagt agctactttg
    22861 cgttatgaaa agaaaacacc tacttgcttc aagccggaac gattttgcgt agcaaaagaa
    22921 taccgaggaa aagggttagg cggtcagttg cttgaagctg ctgaaaaacg ggcggtttca
    22981 gaaggttgtc agactagtta tgtagtagct gaaatgacgg ctgtagattt ttatgaaaaa
    23041 caagggtatc atacttgcac agaacctttt attgaagatg aagtactttg tatcggaatg
    23101 aaaaaaagtc tagcttaaaa acaagagatt gtgacacgcc ttttgtcaca acctcttgtt
    23161 ttttatttat gtgaattttt cttattttta cgagcagtac ggctttgctg tctgcgttcg
    23221 attttgcgtt cttttttatt tttttctgca attgcccaat cgattttttt cttataacca
    23281 ggcttgatct ttttcttttt cttcttcaca agaccaatca gcgtgggatc taatacatct
    23341 tttgattttt ctcgtttggc acgtctatta cgatcgtatg ttttgacgat ttccccgttt
    23401 ttgatttctt ttggttcaaa tgagacgccc aatttttcta tcgcatcaat ggcttgctca
    23461 tcatttggcg aatataacgt aatggccgtt ccttttaagc cattacgtcc agtacgtccg
    23521 acacgatgga taaagaagtc tagatcttct ggaatttctg cattaataac atgagagaca
    23581 ccttcgatat cgatccctct agcagctaaa tcagtcgcta caacatattg atactccaga
    23641 ttctgtactt gtcgcatcac tcgttttctc tctcttgggg tgatgtctcc atgaatcttt
    23701 gctactttca agccttggtt tttcaagtaa tcggcaattt catccacatg ctgttttgta
    23761 ttggcaaata cgattgctaa ataaggatga ccaattgtca gcagttgata aatcatttga
    23821 ttctcatctt taccttttgt tgaaatcagc caattttcaa tgtcttcaga aataactgct
    23881 tttggtttga tttcttcaat gattggattt tccatgtatt ttcgtaagaa tggacgaagt
    23941 ttttctggaa tagtggcaga aaaaactaaa aactgcagct tttctggtaa tcgacttgca
    24001 atttggtcta cttcatttaa aaaacccata tccaatgtca tatccgcttc atcgacaaca
    24061 aatgcaaatg cggtgtgaat cttcagcgct tgttcattca tcatgtctaa tattctacca
    24121 ggcgtgccta ctacaacctg tggctgttgg tgatgcagac gatttaattg acgctgtttg
    24181 tctgtaccgc cgacaaaatt cgagacacgg atctccggtg tagaaaattt agcgatttgt
    24241 gtagctgctt catagatctg tgcagctagt tccctacttg gagctgtaat aacaacttgt
    24301 acttcctctt tttctgcatt gattttttca aacaaaggca acaaaaacgt atgtgttttt
    24361 cctgtaccag tttgagattg cccaatgata ttttttcctt ttgcaattaa aggaatcaaa
    24421 cgttcctgga cctctgtggg ctgtttgaag ccctttgcgg ttaacgcttc attgataaac
    24481 ggtttgaagc ggaatatatc aaaattattc attcgtgcac ctcttctctt taaattatca
    24541 cgactttggc attataacac agacaaagcg tttttgctca gctaatttcg agaatcatca
    24601 tcctttgttt ggccaatcgt gctaacagta tatgtgtgac tagctttttc ataaaacaga
    24661 atccgttttt ttctcaaaaa tgatagcaag atatttaaaa taaaaatacc ttgtagtaac
    24721 aagacaccga gagaaatgaa tactgcactc atctgcaaca ccgtattcgt tgtcgaattc
    24781 atgatttgct gaagcaactg ccccaaccag cttaaaccat aaaaaagaaa gtacagatac
    24841 ccgtagcgga tcatcaatgc ttttaaagaa attcgttcgc ggtcttcttc caccaaacgg
    24901 atacgcacaa tttttttgcc aagtgtccat ccattggtta taaacggtac aatcataaag
    24961 tataccccta cttctaataa aagccaccag atatgagaag taactgcccc tgaatcgaac
    25021 gcaattttaa agaaaatcgc cgctaccaaa ttaataagag ataaaaatgt ccagtcgatc
    25081 aaataagcga ccaagcgtcg agtcaatgag acagtctttc ctttttcata agaaagttca
    25141 tccattcgtc ttcttgtagg aagcataaat gtaaataacg gcgtaacgac aaaaccaatg
    25201 attccaccca atgtatttgt cactaaatca ttcacatcaa ataagcggta tggtcttggg
    25261 tagataaaat aaagtccact tagctgcgtc aattcaaaaa atagagaaag gcagaaacta
    25321 gcaatcatcg ttttcataaa agaaagccgg aaataatatc ttaaataaat gccaaacggt
    25381 atgagtaata aaacattaaa agctggttcc aaaaatacac tctgcttcat tgccggcagg
    25441 taggtactag gttcagacaa gaccaatacc gtgttgtctc tgaaatgttg ccatgaagca
    25501 aataaatgca gctccatctt tggccctgtc atttgggcaa ccgtctcctt tgccggaagt
    25561 ggtaagatca ccaaaaaata cgcacacaat aaataaaaaa cgaaagaata caaaataact
    25621 gcccttgtta ctagaaatgt tccatacttt cgatattcat aaataaaaaa aacagatgag
    25681 atcgctaagg ctaaaaatgg aaaaacaatc agcgcaaaac gaatcggttc gatataactt
    25741 gccatatgac tcctccttga ttctttgtat ctctcttatc ttagcagtcc tttgctgttt
    25801 caagcaagta acgaggctat tatttctcat ttctttcaaa gtaaagtaag ataaacacaa
    25861 agggggtgtc gagatgctga atatgtactt tgtttttggc gtacctattt ttttactgtt
    25921 cctttatgct acgatcgctt atgtaagaaa aagaactaca attcattatt taggatttat
    25981 tctcctgatt atttcgggtt tcatgctagt cttcaatctt cagacttggc agcaagcact
    26041 tcttgaaatg gataaaatga ctccacatgc tttgtctaaa gtgcttggct accctgtcta
    26101 tctgatttgg cttcccattt tcatttctgg ttgcttggtt ttattaaata tttatcgcgg
    26161 cgtacgccga atagttcagt tacgcaaatc aaagtaaaag acagaaatcg gttgagcccg
    26221 atttctgtct tttactttgc tatttcatcg tagacaaaac gacatttacg tgatcaaaat
    26281 taacgagtac ttttgacatt tcttccacgt actcctcatt taaataaaat attttgtttg
    26341 gcagagaagt gcagttctaa taaaattcga atgttttagc tatctacgtt ttaaatagag
    26401 ataaatcggg ataccagcaa gtgtcaatac tatccccatc gaagctaaaa aggtctgagt
    26461 gataagcgtg cttgatacga taaaagtccc tccaatgatt gctactagtg gaataaacgg
    26521 atacattgga actttgtatg gtcgaaacaa atcaggttct tttttacgaa gaagaatgac
    26581 accaacaaag accatcgtat aaaaaatcca aatgacaaag atcagcatat ccgttagtgt
    26641 atcaaaacca ccgatcatca tcatgccaat agcgatgact agctctaaga tacctgcggc
    26701 tacaggaact ttagttttat catgaagttt agcaaagagc ttgctaaaag gcaggttgtt
    26761 ttcttttgcc ataacataag ggagacgcat tccagtaagg gtatagccat tgatggttcc
    26821 ataaacagaa atcaagatac caactgtgac cagtcttcca ccgaagccgc caaaaatcat
    26881 ttttgcaacg tcacttgccg cattgctgtt tcctgcgact ccgtcgatag atgcggttct
    26941 taagaacacc gcatttacta ataaataaac gatcatgatt ccgatgattc ctaaagaaat
    27001 tgcttttggc aaatctttcg ccggcttttt caattctccc gaaatatttc ctacgtgtat
    27061 ccaaccgtcg tatgcgaaca tcgttgctag caagcccgct cccaaggctg agaaaaatga
    27121 aagattttct cctgcttgga tcggaaacag ttggaagtct actccttctt gtcggaacaa
    27181 accgaaaatg acgatcacaa acaaaggaat aagcttgcag acaagcgtaa tcgactgaaa
    27241 agcccctcca gctttagaac ctaaaaaatt gatcaacagg atcgatacag cagctgtaac
    27301 agctacggga acaatcattg actgggaaag accaaataga ttgacgaact gtgtaccgaa
    27361 aatgatcgac aaggctgcta catttgctgg aaaataaatc acgacttgcg cccagcccaa
    27421 taaaaaagct gctgtatttc cgtaaatccg ttcgatatat ttaatcatcc ctcctgtttc
    27481 tggaatagca gctgccagtt cagctcctgt tagtcctgca cagacagaaa tcataccacc
    27541 gagaaaccaa gaaaacatat gcaaactggc ggatcctgta acttctgcta cgcttgcggc
    27601 tttaaaaaat acccctgcac cgatcactgt gcccataaca gtcgataaag cagtaaaaaa
    27661 tcccatcgta cgttttaatt cattttctat ttttacttct tttactggcg tggcttgata
    27721 ttcttccatc ccttgtcccc tttcatacca tagccaatcc caaaagtata aaaaactttt
    27781 tggatgtggt tcttgtttta taccgtctct accaattttt ctttcagttt ttccaacata
    27841 tcctttgtca tcacatctaa gtcgtatttc ggcgaaaagc cccattcctc ttgcgcacaa
    27901 gaaacatcta gtgaatttgg ccaagattct gctattgact gacgtacagg atccacatca
    27961 taatccattt caaaatctgg tatgattttt tgaatagatg ctttgatctc ttccgggtca
    28021 aaagacatcg ccgatatatt gaaggcattt cgatgaacca atcgatcagg atctgcattc
    28081 atgagtttta taatggcatc gatcgcatct ggcatataca tcatatccat tgatgtacct
    28141 tgatcaatga aactagtgta ctttccttct ttgattgctg aataataaat atcgactgcg
    28201 tagtctgtcg taccgcctcc tggcaaggtt gcataagaaa tcaatccagg gaaacggaca
    28261 ccacgagtat ctacgccata tttcacataa taataatcac atagtagttc tccagctacc
    28321 ttcgttaccc catacatggt tgtaggacgc tgaagagtat cttgtggggt atgatctttt
    28381 ggagtatttg gaccaaacgc gccgattgaa cttggcgtaa aaaatttcag ctggaattct
    28441 ctagcgactt ccaatgcatt gaccagccca gtcacgttca aattccaggc taattgtggt
    28501 tttgcttctg caacagctga caaaagagca gccaaatgaa tcaatgtgtc cacttgatat
    28561 tgttctgcta tgctgcgcat tttttcgtaa tccattacat ctaggatctc aaagatatcg
    28621 tttttctcaa tgacttcagg catgcgtata tctgtcgcaa tgacattttc actgcctagt
    28681 tcttgacgta gccgtgtgat caactctgaa ccaatttgtc ctaaacatcc tgtcaccaat
    28741 acttttttca tgatgatcct ccctgatctt ttttactact atcttcagat gattaacaat
    28801 atatgcgtga acatgttctc taattttaaa tagacgaaaa aactaaattt agttaaataa
    28861 tttccatttc ttttcctatt ttttgataaa ctgctactgc ttcatcaagc atttccctcg
    28921 tatgttccgc agtcggcata tttctgatcc tgcctgtccc aagaggcaca gttggataaa
    28981 cgattggttt agcataaatc ccatgttcga tcagcttctt agagaaagtc tgagtcactt
    29041 tttcgtctcc caaaatgact ggagtaatag gtgtttcaga cataccgatg ttatagccta
    29101 cttttttcaa ttcttcttta aaataattag cattttccca aactttttcg acaagttccg
    29161 gatgttcttg catcaaagta atcgaagcca atgcagctgc agctgcacct ggagttagcg
    29221 aagtagaaaa taggaatggc cgacttctag ctttcaacca gtcgattagt gttttcgatc
    29281 cagctacata acccccaaca acacctatag ctttcgataa cgtgcccatt tgcatatcga
    29341 ttttgtcgct taatccaaaa tgcttgacgg ttcctgcacc ttttccagtt acgcctgagc
    29401 cgtgcgcatc atcgacgtag gtgatcagtc catatttttc aacaatcgga ataatttccg
    29461 gcaatcgtgc aatgtctcca tccatggaga aaacaccatc tgtgatatac atgatttttt
    29521 tatatttctt actttctatc gcttcctttg cttttttctc aaggtctttc atatcttgat
    29581 gtttaattcg gatgattttt gctccggata agcggcaacc gtcaataata gatgcatggt
    29641 ttaattcatc cgataagata gcatcttctt ttgtcattac tgcagagata gcgcccatat
    29701 tgcagttgaa tccagattga aaggcgattg ctgcttctgt ccctttgaat tctgcgattt
    29761 tttcttctaa ttgctgatgg attttcagcg aaccattgat cgttcggact gctcctgctc
    29821 ctacaccata agtttcggtt gcttcaatac atgctttttt caattcttcg cgattggcaa
    29881 atccaagata attgttggat gctagattga tcatcttttt tccgtctacg atgatacaag
    29941 caccattttc actttctagt atgtcgatcg tattgtataa ccccttttct ttcagttgat
    30001 ccaattcttt ctctaaatac attgttagtt ctttggaaga cattgtctcc acctccatat
    30061 tcatctgtct tgcagaacat accaaacata tatcgtgtca ttcatctaca aagcaagtat
    30121 gctatatttt tgataacgct tacactgatt atgagaatta tttaaccaca ttttttattt
    30181 acagta
//
LOCUS       DASJTM010000030        29965 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJTM010000030 DASJTM010000000
VERSION     DASJTM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.030"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            281..1981
                     /locus_tag="U4B29_001230"
     CDS             281..1981
                     /locus_tag="U4B29_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106190.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2083..2916)
                     /locus_tag="U4B29_001231"
     CDS             complement(2083..2916)
                     /locus_tag="U4B29_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN2106191.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3220..5385)
                     /locus_tag="U4B29_001232"
     CDS             complement(3220..5385)
                     /locus_tag="U4B29_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2106192.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5645..7939
                     /locus_tag="U4B29_001233"
     CDS             5645..7939
                     /locus_tag="U4B29_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2106193.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            7932..8597
                     /gene="pgmB"
                     /locus_tag="U4B29_001234"
     CDS             7932..8597
                     /gene="pgmB"
                     /locus_tag="U4B29_001234"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2106194.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            8590..9597
                     /locus_tag="U4B29_001235"
     CDS             8590..9597
                     /locus_tag="U4B29_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2106195.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9619..10638
                     /locus_tag="U4B29_001236"
     CDS             9619..10638
                     /locus_tag="U4B29_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2106196.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(10919..12286)
                     /gene="nhaC"
                     /locus_tag="U4B29_001237"
     CDS             complement(10919..12286)
                     /gene="nhaC"
                     /locus_tag="U4B29_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2106197.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(12395..13816)
                     /locus_tag="U4B29_001238"
     CDS             complement(12395..13816)
                     /locus_tag="U4B29_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106198.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(14059..15936)
                     /gene="tdc"
                     /locus_tag="U4B29_001239"
     CDS             complement(14059..15936)
                     /gene="tdc"
                     /locus_tag="U4B29_001239"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2106199.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(16227..17483)
                     /locus_tag="U4B29_001240"
     CDS             complement(16227..17483)
                     /locus_tag="U4B29_001240"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN2106200.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(17595..17800)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18063..19364
                     /locus_tag="U4B29_001241"
     CDS             18063..19364
                     /locus_tag="U4B29_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2106201.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(19433..20032)
                     /locus_tag="U4B29_001242"
     CDS             complement(19433..20032)
                     /locus_tag="U4B29_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2106202.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            20286..21167
                     /locus_tag="U4B29_001243"
     CDS             20286..21167
                     /locus_tag="U4B29_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106203.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            21180..22790
                     /locus_tag="U4B29_001244"
     CDS             21180..22790
                     /locus_tag="U4B29_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN2106204.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(22867..23526)
                     /locus_tag="U4B29_001245"
     CDS             complement(22867..23526)
                     /locus_tag="U4B29_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2106205.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(23809..24474)
                     /locus_tag="U4B29_001246"
     CDS             complement(23809..24474)
                     /locus_tag="U4B29_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN2106206.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(24621..25226)
                     /gene="dhaL"
                     /locus_tag="U4B29_001247"
     CDS             complement(24621..25226)
                     /gene="dhaL"
                     /locus_tag="U4B29_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN2106207.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(25254..26264)
                     /gene="dhaK"
                     /locus_tag="U4B29_001248"
     CDS             complement(25254..26264)
                     /gene="dhaK"
                     /locus_tag="U4B29_001248"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN2106208.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(26239..26685)
                     /gene="dhaM"
                     /locus_tag="U4B29_001249"
     CDS             complement(26239..26685)
                     /gene="dhaM"
                     /locus_tag="U4B29_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN2106209.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            26788..27108
                     /locus_tag="U4B29_001250"
     CDS             26788..27108
                     /locus_tag="U4B29_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN2106210.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(27220..28089)
                     /locus_tag="U4B29_001251"
     CDS             complement(27220..28089)
                     /locus_tag="U4B29_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2106211.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(28185..29489)
                     /locus_tag="U4B29_001252"
     CDS             complement(28185..29489)
                     /locus_tag="U4B29_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2106212.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(29486..>29965)
                     /locus_tag="U4B29_001253"
     CDS             complement(29486..>29965)
                     /locus_tag="U4B29_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106213.1"
                     /translation="SILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLPFIWVFFPLTR
                     PERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNLYKKVNKSFEY
                     EDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADLYKENGVELK"
BASE COUNT         8777 a         6265 c         5446 g         9477 t
ORIGIN      
        1 tttgagtaat aagagcgcta gtcagaaaat agcttctcat attttctagg ctagtgagct
       61 tattaccgag aagcagctga ttgaacaccg tttaccgaga ggtcatgaaa aagctacttt
      121 gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag atgcaacaaa acttatgtcg
      181 catcctctct ccttagaact ttcacttatg tttttcttgt aaaattcata aatattttca
      241 tgaattttct aatttcttgc ctacctctcg cataggatat atgatgaatt tatctaaagt
      301 ttgcaaagga gttcgacaat tgaaaaaata tggcccattt ttagcaattt ttttcattgc
      361 tttggcattt ctcatgcctc atttattcac acataaacta attttgggtg ctgatgcccc
      421 attccattac aatcggttct atgatgccgc gcaacaaatt aaatatgggg atttccatta
      481 ttttccttcc ctttatggat tccagcaaag tggcaggatc gtgaatgccg tttacggccc
      541 atttttcgct tatttacaag gattgatcgt gttgcttgca ggtagctggt ttcattatca
      601 gattctttca aatttcattc tttatcttat ttcaggattt tctatgctga ttttactgag
      661 atatgtcaat gttcgaaatt gggtaagtgt accagtagcc gttttattca tgagtacgta
      721 ttctattcaa tactggtcta tcaaccaagg attcactagc tgggggactt gtttttatcc
      781 tttatgtatg atccctattg ctgatttcgt ccttcataaa aagttccctg ttatcaaagt
      841 cgctgtctca gtgggactta tgacacagat ccatttactg tcttcgatca tgttagcttt
      901 gatgtatatc cctttttatc tttattactt ttttaaccag actgaaaaag gaaaagcact
      961 gcttcaactg attaagtcgg tcttgcttta ttttgttctg acagcaaata tttgggcagg
     1021 attagctatt gtttacagtg ggaataagat tcttcctccc tttgttaaca gagaaatgag
     1081 tcaaaaggcg atagatctgg atggcagtta ttggctccat taccctaagg cctttcctat
     1141 attgatatta tcaggttttt tgctttttat tttctttcaa caaaagaaca ccttattcca
     1201 aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt tctactagct tgattccatg
     1261 gacgacattg atcgaaaaaa atgtgccttt tattagtttg atccagtttc catttcgatt
     1321 tttcgtgcca tttacgctat tgcttctact ttaccttgcc ctttcactga accagtggtc
     1381 tgaacaaaag tggttccgtc ttttaagtat atttggcatt cttatttttt cagcacagac
     1441 gatccagaac ttataccagc atctggaaaa gtgggaaaat gaaacatttg tcagtcgaca
     1501 tacttatcta ttcgatacgc cggaagaagc aaggaaaaca ttcttcagca aagatatgtc
     1561 taccagcttg ttcgttttcc aaaaaaccac acctgactat ctgccaatct ataaggagac
     1621 aaaagacaat aaatacgacc ggtataacga agaaatacta gaaaacgaaa caacttttat
     1681 aaaaacacat ccaggcggtc ttttgacaat caagtggaaa aacactgggg acaaaactgt
     1741 tcatatccct gttatcgtct acgatcggac tgtgctacag caaaatggaa aaacattgac
     1801 tgattatgaa gtaacagata tcgggacccc aatcgtgaag cagcaaaaag gaatcaacga
     1861 gctcacgctt cattatcaaa cgccaatcta tttctatttc attctttctc ttactttgat
     1921 tggctggttt accttgctat gtttatttat ttatcataga tacaagcttt tacgtgctta
     1981 ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa acggtataat cgtttggaga
     2041 gttttatttt aatcagtttt tttatgttat atgttatatt ttttattcgt tcacatctaa
     2101 ttcgatttcc accccgtagt gatcactgat gattggacta tcgatcccat caaaaatccg
     2161 ttgatatttt ttaacctgca ttccttcagg gacgaaaata aaatctatac gcaatttttc
     2221 tgtattttgt tcccaaccat ctattttctt ttccacagta gctgcttttc ctttttcatt
     2281 cgcgacaaca tacgaatctt gaagaagaag gcaactttct cttacagtat aataaccttg
     2341 agagtctact ggattattga aatcacctag gaaaaaaagt ggtacgcgag tttccaaaaa
     2401 gtagttttcc aagtttttcc actcaaaata aaagccactt tttttatctg tccaccaaga
     2461 aaagtggcag ctcgagacaa aaatgtcctg atcatctatc tttgttttcc caatcaagat
     2521 cttgcgtgtc tgataatctt ctttcttttg aaattgggag acggtaagga cctgactttc
     2581 caatgttttt ttcgtaagca gtgcatttcc ttcttcatat ttatcataac cgatgtggct
     2641 catttcccaa ctccaaaaat actcttttcc gtgttccttt aaaagctgaa ctaaacggta
     2701 ggcaaaatta tctttacgga taggtacttg attctttacc ggacagaact tcattagctt
     2761 cgcaggttct acttcttcag attccagtaa ttggttgatt tcttgtaaag caatcacttc
     2821 ataatcttct aacaggatct tatctgctaa ttcttgcaat tttttctctg gttctggctc
     2881 aagccagcta tgcgtgttga gtgtcatgaa tttcattggt tttctccttg tcttcatttc
     2941 gaaacaaaag ggttgtgaca aaatttttgt cacaacctct ccagcaaata gtgttctcct
     3001 gctaactcct cagtaataag ctcaatggac caggagctat tttttgtttg gctgttttta
     3061 tcacttggtg cagggcagct ttttcacgac ctctccacta aacagtattc aaactgctgc
     3121 ttctcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
     3181 ttatgacttg atgcaggaca gctttttcac aacctctcct tacattttaa tggagccgat
     3241 cactttacca gctgtcactg tacctaattg cttgatcact acttcttcta ctttatcgtt
     3301 tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt tggatcacat caagatccat
     3361 ctcagctaat ttcgttcctg ttcctacttc ttggccttct ttgacagcaa tctcaaaagc
     3421 tggctgattc atttggactg tatcgattcc catatggatc aaaacttcga taccatcttt
     3481 cgtttccagc ccaattgcat gtttgctagg gaaaatactt gtcacttttc cagcgatcgg
     3541 cgcaacaact tctcttgatg ttggaataac agcaaagccg tcacccatca ttttttgtga
     3601 gaataccgga tcttttactt cagataaagg aatcacttct ccagaagctg cggaaactag
     3661 ttcttgttct atacctaagt attgcttact acctgcttgt ccatttcctg tagaaaccat
     3721 ttctggctct ggaatctctg ctcctgattg cagcagatct tcgatatctg atttcaagac
     3781 atctgctttt ggtccataaa ctgcttggac tccattgtct ttgacgatca atcccatcgc
     3841 ccctgctcgt ttccaggctt cttcagtccc tactttttct cgatcattta cacttacgcg
     3901 taaccgagtc atacaagcat cgacatcttt gatattttct tttcctccca gcaaatgaat
     3961 gatctggacc acttgctgat ctgctgttcc tgttccgctg ttagatacaa cggtttcttc
     4021 actgtcattg tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa
     4081 gaaatttgct aagaaatacg taacaacacc aaaagcgatc acacacaaca cgaagttgat
     4141 aagatctcca cctagacccg ctttgatagc taacggtgtc cgagtcagca attcgatatt
     4201 cccaaatgaa tggacccgca atggcaagat atctgccatc gcaaatgcag ccccttggat
     4261 cactgaataa atgacatata atggcacagc agcaaacata aacatgaact ccaatggttc
     4321 tgttacacct gttagaaaga cagccaacgc tgctgaaaaa tacattgatt tatatcttgc
     4381 tttcttatca ggatcgacat tacgatacat cgcaaaagcc attcccatca agatacctga
     4441 agaaccaatc atttgtccta ctttgaaacg agccggcgtc cagttatgca acacaaactc
     4501 atattgcgac atatcccctg cacctttaag attcactaaa tcagtggccc aagccaacca
     4561 taacggatct tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga
     4621 tccgccaagc tgtgtatagt tgataggaat cgtcagcata tgatgcaatc caaatggcaa
     4681 tagtaaacgt tctaatgttc caaataaaaa cggtgctaga atcggtgcgc tttcttgtga
     4741 ttctgcaatc cataaaccaa aattattgat tccagcctgt acgtttggcc acaccacagc
     4801 aagaatcaat gccacgattg ttgaccataa gataacaaca aacggaacaa agcgctttcc
     4861 attgaaaaat gacaaagcat ccggcaattt tctataatta tagtacttat tataagccat
     4921 tgccccgaca aatccagcga tgatccctac gaatactccc atattcaaag ctggtgcttc
     4981 caatacactt gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa
     5041 tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag atagccccag tgatccgatt
     5101 gattaaaata aatgagatcc cagctgcaaa tgctccgcct gctctttctt ttgcccaact
     5161 tcctcctatt gctaaagcaa ataaaatatg aagattacca ataattgccc aaccaatatt
     5221 ttcaattact ccgcccgttg tgacaagcgg cgtccaatca ggattgatca agggaattga
     5281 tttccctata ctgatcatca acccagctgc cggcataact gctaccacaa ccattaaagc
     5341 ctttccaaat ttttgccaaa actcaaaact aaatatcttt ttcattccgt ttagctcctt
     5401 tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt tcttttttgt aaatgcttta
     5461 ttttttattt ttctgtctat accattctat aaaacctata atacgtgtgt tcccttctat
     5521 aattttctcc taaaaattta tttttttaag cttttttcta tgaaatattt tcttttgtct
     5581 attgacttgt gcaatcggtt gcgtttatta tgaacgtata aaacagctca ttaggaggag
     5641 aaaaatgaaa cagatcaaac gactatttgc tattgatcca tggaaaatca gcacgacaca
     5701 attagataaa aaaaatcttc gactgcaaga atctttaaca agtattggaa acggttatat
     5761 ggggatgcgc gggaactttg aagaaactta ctcaggggac caccatcaag gtacttattt
     5821 ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg tggaaaaatg gttatccaga
     5881 atactttgga aaagtgatca acgccttgaa ctttatcgcg atagatattt cgatcaacga
     5941 tcagctgatc gacttggctt ctttggaacc agaagacttt tactgggaat tagatatgaa
     6001 aaatggtgta ttatctcgta cattcacaat cactacttct actaataaag tacgcatttc
     6061 cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct tatatctatt taactataga
     6121 aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa
     6181 cgaagacagc aactatgatg aacatttttg gaatgagatt gatcgtggaa ctatagaaga
     6241 aatcggctat ttgacgacta aaactattcc aaacaacttt gggattgaac aatttactgt
     6301 tactgcagca atgcgccatt tcgttaatgg aaaatcagtc atccctgact atcacgaaga
     6361 tactttagca ttacatggtt gcttgtctgc agaattatct caaggtgaga catggtcagt
     6421 ggaaaaagaa attatcgttc ttactagtag agatattcca gaagcgcagc aagcagaaaa
     6481 agctgtctca gaattaacga accagacaaa caattatatc caagcaaaag aagagcaaac
     6541 agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc
     6601 acaacaaggt attcgattca atttgttcca actattctct acttactacg gcgaagatga
     6661 acgattgaat atcggcccca aaggattcac aggagaaaaa tacggcggag ccacttactg
     6721 ggatacagaa gcgtatgctg tccctcttta tcttgcttta gccaaacctg aagtaactaa
     6781 gaatctgctg aaatatcggc ataatcaact gccgcaagca atccacaacg ctcagcaaca
     6841 aggattaaaa ggcgcgcttt atcctatggt caccttcact ggtgtcgaat gccataacga
     6901 gtgggaaatc acattcgagg agattcaccg aaatggcgcg attgcctatg caatctacaa
     6961 ttatacgaat tacaccggcg atacggaata tttgaaaaat gaaggactgg aagttttagc
     7021 agaaatcgca cgcttctggg cagaccgtgt ccatttttca aaacgagcaa ataaatacat
     7081 gatccacggt gtgaccggcc caaacgaata tgagaacaat atcaataaca attggtatac
     7141 aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa aattatttaa cttatcaaaa
     7201 agaagctgct atcactatca gcgaagaaga actagccaaa tggaaagata tcgcagagaa
     7261 catgtacttc ccagttgacg aagaattagg aatctttgtc cagcatgaca cattcttaga
     7321 taaagatctc atgcctgtat ccgatattcc cacaagcgat cttcctctga atcagcattg
     7381 gtcttgggac aaaatcctta gatcttgctt catcaagcag gcagatgtac tacaggggat
     7441 ttatttcttc aatgatactt ttacaaaaga agaaaagcaa aaaaactttg atttctatga
     7501 accaatgact gtccatgagt cttccctttc gccaagtatc cattcgattt tagcagcaga
     7561 attaggaatg gaaagcaaag ctgtagaaat gtaccagcga acagctcgct tggatttgga
     7621 taattacaat aacgacacag aagacggctt gcacatcact tccatgactg gtagctggtt
     7681 gtcgattgtc caaggatttg ctcaaatgaa aactgcccat gatcagttga gattcgcacc
     7741 atttctgcca aaaacttgga aagcttattc tttccatatc aattatcgcg gacgtttatt
     7801 gttcatctca gtcacagatg aacaagttac tttcgagtta ttagaaggtt ctcctctaga
     7861 aatttacgtc tatgaacagt cttattcttt agcggatcgt ctagaaataa cgctaagaaa
     7921 ggaggaagca aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt accgatactg
     7981 ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt acgatcgacc
     8041 acacattcaa cgaacagcta aaaggcgtca gccgagaaga ctcactctct ttgcttttag
     8101 cttatggcgg aaaagaacac tctttttcaa aggaagaatt tgcagaatta gcgaaaagaa
     8161 aaaacgatta ttatttagag atgatccaga cgatcgaacc aaaagatgtc ttccccgggg
     8221 tcgtgccttt actggattct ttaaaagaag aaaaaatcaa gattgctctt gcttctgcaa
     8281 gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac accttacttt gatgctattg
     8341 ccgacccggc agaagcaaca aacggaaaac ctgcgccaga tatcttcctc cttgcagcaa
     8401 aagctgtcgg actaaaagca gaagaatgta tcgggatcga agacgcgcaa gccggtatcc
     8461 aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa gaccttggag
     8521 aggagatccc gcttgtccct gatacgactt ttcttacgat cgattacttg aaagaaaagt
     8581 ggcatgacca tggataatcc agagatctcc gtcgtaccag ttccagagac tgaaataaag
     8641 aaaatcacat tgaagaacga ctacttagaa gtcgttcttc tcaattacgg tgcccgcctg
     8701 caccaaatct tcgcaccaga caaagaagga aaatcagaaa atgttttatt gtcttacgat
     8761 cgtttcgaag atgttctaac agacaaaagc ttttttggag caactgtcgg acctgtagcc
     8821 ggaaggatcc gggatgcttc ttggggtgag catagattgg agaaaaactg cggatcacac
     8881 catatccacg gtggcacgaa aggctggtct ttccaatttt gggaagtaga agttttcaaa
     8941 actccatcat caataggtgt ggtcttttac ttaaaagatg aattttctac ctatccagga
     9001 ccaatcactg ctacgatcac ttaccgttta acaaaaaacg aattagagat gacaacctct
     9061 gctagtagcc ttgttgaaac catctgcaat ccgaccaacc atgcctactt caacctttca
     9121 ggaaatggaa caagagacat ctatgaacat cagttaacgg tctttttaga tggtgtgctt
     9181 gaattagatc aagaaaaact gcctactgga aattggacaa aaaaagaaaa actgccaatc
     9241 gactttagaa aatccccaac actgcaagag atccttgcat gttatccaga tggactagat
     9301 gatgtatttc ttttgcatca tcctaggctt tccaaaactt ccttacaatt attcgagaag
     9361 cattctggtc gccaaatgac tatagcaact tctaataaaa gtatggtatt attttctacg
     9421 actggattcg aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa ctacggatta
     9481 gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg atcgatcaac
     9541 ctccatcctg gtcaagaacg gatcagtcag acgatttatc ggttctctgc acaataacaa
     9601 gagaaaggtg tttttatcat ggctattacc gtaaaagatg tggcgaaaaa agccggtgtt
     9661 gccacctcga ccgtctctcg tgtcatcaac gatcatccta gtatttccga gacaacaaag
     9721 aagaaagtcc gaaaaattat ggatgaacta ggttatgttc ctaacatgac tgcccgcaat
     9781 ctaggaaaac gcatttccag tgccatcggc gtaatcttac cccctcttga ttcaaaagag
     9841 cggatcggta acccatttta tcttgagatc atggaagcaa tcaacgaaga agcgcggaat
     9901 tatgatatga ctacagcaat cgctactgct aaaaattttg aattgctgtt agagaacgtc
     9961 cagcgtatgc atttacaaaa acaagtagat ggttttattc tagtgtattc agatagtcaa
    10021 gatactgtca ttgattattt ataccaaaac cacatccctt tcactttgat cggacagcct
    10081 tatcatcatg agaacgaaat cgtttatgta gataatgata accagcttct aggtaaacaa
    10141 gcaacagaat ttttgatcca gaatggacat aagaatatcc tgtttgtgac aaacacgact
    10201 catgaaagtt tgtatttcga acgatatttc ggttatcaaa aagcaatgat gatggctggt
    10261 cttcctgcgt tatccagtgt agtactagag catccggagg attatctgtc ctttgagaaa
    10321 acgttgaaag aaaacgaagc cacagcattg gtggtcattg acgatatctt cgcccttcgc
    10381 acgatgcagt tggcgcaaat gtatggctat gtcattccag agacactctc cgtcattagc
    10441 ttcaacaact cgatcttctc aacattaaca cacccttacc ttacaagtat cgatatcgat
    10501 atctccgaat taggacgtgt cgctatgcaa aagctgcgcg aattgatcca tgaagaattg
    10561 agcagtggcg tacgtttggt catccctcat cgcttgatca aaagagagac agtcgttcct
    10621 ttagaaaacc catactaaat agctgactca ctaaatacgc tgaccattta tcctatggtc
    10681 agctttttat gtaaaagaag atactacttc taacacattg tttatctgta aaaaagagcg
    10741 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
    10801 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg
    10861 agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tccttccttc
    10921 aattttttac acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa
    10981 gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct aactttcatc gttcccataa
    11041 tgaatgtgcc gctgactccc cacggtacga gagaattgac ggtcgcacct gaatccgcta
    11101 gtgttcgtgt caaataggtt tttgataagc caacgtcatc aaaagaacgc ttgaatgttt
    11161 ctcctggaag aatgatggac agatactgtt ctccaacgat cagattgatt ccgatacagc
    11221 tcaaggcagt aaacccgact aaacgagaag gtcggtccat cttttctttt aattcttgaa
    11281 cgatggtatc aacgatcaga tatttgatca gtaacccacc taatgccaat gcaagtaaaa
    11341 tcaaactagc agaacctaac atactcatga tcccgcctcg agaaagcagt tcatctagct
    11401 ttggatcagg tgtatttgcg acataaccat tcatcaagat gtctgctgct tttccgaccg
    11461 tcaaactttt ctcatggata aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg
    11521 tagggatagc cggaattttt ttgattgccc ccccaaataa aataactagt ggaatcaatg
    11581 tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg atactagcta
    11641 gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg
    11701 ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact cctccaatac
    11761 ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa aaggcaccgg
    11821 aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg atccccaaaa
    11881 aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact ccgacaattg
    11941 cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta aatccataaa
    12001 ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca ccaatcaaaa
    12061 gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg tcatggatct
    12121 catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa gctgcaagga
    12181 tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt gccaacaaca
    12241 cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc gatctcctat
    12301 ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct cctactaaat
    12361 cagcgacaaa acgtcggctg aaagacttat ttttttattt ttcttctaaa cttttttcta
    12421 cctcaatccc ccaagaatct ttcttgaagt gattgatgat caatggaata acgaacatga
    12481 ccactgtgat agctgctact aataatgcat cactcaatgt cgctgtacct aatgtagcaa
    12541 atacgacaac gatgatagag aagatcagca tgcagctgac tacccaagct aatccgtttc
    12601 cgtttttacc aattcggtaa ggacgttcga attctggatg tttcttacgc attttaagca
    12661 atgaaacagc aatcaaaatg tacacgatac aataaataac agttgtagca tttgtaagtg
    12721 tcaagaatac agcacttacg tcatccatta caccataaag caaagcaaat aatgaaataa
    12781 cgattgattg tgttagaacg acatttctag atacgccgta tttgttttct ttatggaatc
    12841 cgaattttgg tggaaggaat ccttcacgag ctacttggat gattgttttt gatggtccag
    12901 taacccaagc tgataattgt aaaagcacac caataaatac catgaagctg aagatatttc
    12961 cgatcacttc tggtaagcct aggatttgac agtcaatcaa gataggctgc gtgatatttg
    13021 ctaattccat ttttccatct ggtacgacat ttgctacaag catcgcattg atgacattca
    13081 ataaaactaa tccgatcaaa gcgataaata cgccttttgt ataatttttt gttgcgtctt
    13141 tcaaacgagg catgtaaaca gaactcattt cgatcccaac aaaaataaat gtgataccag
    13201 ctaagtattt caatgaatca atatgttcca agtcagggaa agcttttgac catgaaaagt
    13261 cacctagata gccgtttgag actagtccga ctttgaaggc tgccaaaaca cctaaaacga
    13321 acatgatgac tactgggata tataccccta gccatacacc gatgttcccg ccgacttttg
    13381 ccatatcgaa tttcaagttc aagatcgtga tgatccagta gataactaaa atacaaccta
    13441 aaacgaacca gtgattattg ccaagttcta cattaccaaa tgtgtttcct aacaatggtc
    13501 ctaaagtaga agcgaccatt accatacctg ggaacatttg cacccataat agccatgcaa
    13561 cgacaaatcc ccatttgctg cccagtgctg tttttaccca taactgagga ccgccttctt
    13621 gaggaagcat cgtggaaagc tcaccggaaa ccattgatat cggaccggca aaaaacaaaa
    13681 ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta ggaatactcc
    13741 gaaccgttgc acacagtgcc attgtcagac cgataaaggt cccaagaccc attttactta
    13801 cttcagattt actcaccgtt gacactctcc ttgatttttt ggataaaata acggtgtctt
    13861 tgaagtagtt tcagcaataa acgaaacaat tcagcgaata tttgagggga ttctttgagt
    13921 ttattgcttg aagggtttct tcaaagacaa cctcaaaaca aagggatcga agagcttagg
    13981 tctccccttg ctcttcagat ccaaacatgc gtcagaaaca gttagtattc aagctgtttt
    14041 ctaacatgat taccttgctt attttgcttc gcttgccaat aattggtctg cgtagatttt
    14101 ttccaatttt tcttgaagag ctgctttgat tttttcagca tattcttcaa agtgttcttc
    14161 tttgttcatg tatggtgtca ttacgctggc acgcaataca gttacttttc cggcacggtt
    14221 ccattcttca tctgagaatc ctagttggtt cacaaattgt aatgggctgt tgccatagtc
    14281 tgggatagca aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac
    14341 gtatgaagaa taatcatata cgtcatggtt caatttattc attgcaacta agtcatcgtt
    14401 gcctttttct ttgaatacat agtctaccat attgaaatct ggataagtta atggatgaac
    14461 ttcgatttct ttgtctccta ctttaaatga caagtcattc aagaagttgt agaaacgatg
    14521 tgcaccttcg attgatgcac ccattaattt accatatcct gtgacattca aaggtaagac
    14581 atggtgtgct gcccaaacgc tggctgctgt tgcccctgct tttgaacctt ccaagatata
    14641 agccccaagt aatgctggga tatctgcgcc tttttcaaat acatatgtcg caaagtaaga
    14701 gataacgtct ctcatgcgga tatctttgat gacgatacca ccggcagaat atggaacata
    14761 gcccatttta tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt tatatgcact
    14821 gtgtacttct tctaagatat aatctttgtt ttctgtaaag acattgtatt tgtaatgaac
    14881 atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc
    14941 gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac
    15001 gcgacgcaat gcaacgattt tatcgatacc gtcgattgct ccttcttctg ttgatcctac
    15061 aacaccgact acacctagga ttggtgtttt ttcagctgct agaccacgaa cgattttttc
    15121 aagttcgttg atgtccatgc gatagttgtg gtcaactggc actggaatca cttggtctaa
    15181 accgataccg atgatgtcgg cagcttttag ccatgaatag tgtttagttt gtggaaccaa
    15241 ccattttcct aatttttcta aatgttttcc gctacgtgct gagtgagctt tgatttcgtc
    15301 gattttttct gggacgctgt ctaacagatt catgatttct tctgttgaca agttcattaa
    15361 ttcccaatcg ctttttccag caactagttc aggtgtcact tctttcattg caagcggcaa
    15421 tgatttgatg ttgcgggcat accaaagtcc ttctaagttt gctaaagaac catcagcaac
    15481 gatgtggccc cagccatctt tatagctcat taattttgca aattccattc ctacttcttc
    15541 ttccatttgg cttgttgcag gagaagattc ataggcaacg ttattaccat tccatagcat
    15601 agcaaaattg tacgctagta aagatggcat caatgtttca gagttcatat gaccccagta
    15661 acgtccggca ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag
    15721 aacatccttt gttttattca cagtgtgttc aaaactagca ctgctttttt cttctggcgt
    15781 aataataggc atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt catccaccaa
    15841 ttcattcaac aaagctttat agatttggcc gttctcagct ttgtccccaa tgaatagggc
    15901 atttaggttt agatctttcg acaatgattc actcatagtg aaaccctcct aaaaaaatat
    15961 ttaaattttg tcgagcccta cattacacct gttcttctat tctgtaaact aagttttggc
    16021 ctgattttta atcatatttt aatatgtatc aattatccga attgttcaca cttttgaaat
    16081 aattaaaaaa aacgagggaa tttacaaatg taaactccct cgcaaagatt gtattaacga
    16141 caaccagcgt tctattttag gatgggtttc aaaattggaa tgttgtaata gtatttccct
    16201 ttttcaaggt aaaaacttac taaattctaa tcaattactt tggccaaaaa gtaatttttc
    16261 ttaccttttc gaataaccac aaacttacca tcgaattcat cggatggatt tatgacaaaa
    16321 tttaaatcgg taacgcggtc accgttaata cttatagctc cgtttgaaac atcttcacgt
    16381 gcttgacgtt tagatggttc aatttttgtg gaaacaagca gttccacgat attttcaggt
    16441 gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc ttccgcattt
    16501 agttctttaa tatttccaga gaacaatgct tgcgttattt tttgcgcttc tttcaagtct
    16561 tcttcactat gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc
    16621 tcaggttctg tctctacttt tttcgctaag tcttcgattt cttcttgaga caagaaagta
    16681 aagaatttca agtatttcac tacatcgcga tcatcttgat tcaaccagaa ttggtagaac
    16741 tcaaatggtg aagttttctt cggatcaagc caaacggcac cgcctgcagt ttttccgaat
    16801 ttcgttccgt cagccttcag catcaatggg atcgttaaac cgaacgcttc tgcttctggt
    16861 ccttctaatt ttcggattag gtctaatcca gctgtgatat ttccccattg gtctgctcca
    16921 ccgatttgca attgcacatc gtatgtttta tgcagatgca aaaagtcgat agattgcagg
    16981 atttggtaag taaactctgt aaatgagatt cctacttcca aacggctggc aacgatatct
    17041 tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa
    17101 gagatttttg acaaccagtc gtagttgttc acaaatgtga tatttgcatc tttaccaaac
    17161 aattttctca tttgattgga aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc
    17221 aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc
    17281 agaatatatg gatgatggcc tgccaattgg aatcttttca tcatcataaa tggaatcaaa
    17341 tgtccgatat gcatcgaatc accagttgga tcaacgccgc aataaagtga aatgctcttt
    17401 tcttcaacga gctcgcgaag tctttcttcg tttgtttgct gattaatggc gtcgcgccaa
    17461 gttaattcat caataatatt cattttactg acctccgtgt agttttttta tgaaagcagg
    17521 aacctgctag catatgtaat taattatttc ttcaggatag gataggaaat aaaaaaagtc
    17581 ccttggcata taagccaagg gacgaaatct cgcggtacca cccaatttgt atgtctagat
    17641 atccaacata cctctcgaac atttaacgct gtcgcgtggc aatcactgcc aaagactcct
    17701 gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg accaccagct ttctgaaaca
    17761 ttggctttaa caactacttc gtttcgatct tcgtcttgtt attattaata tgtgtctatc
    17821 atagagcgca actttttctt tgtcaacaga aaaaggatat tttttttaaa ttcctcattt
    17881 tttctttttt gaatccgctc taattccgaa cattttattt tcaattattg ttaaaagaaa
    17941 taactttttc tgtatttttt ctgcaattta tcgagaagtc actttacttt gcctcttatt
    18001 ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt
    18061 atatggaaaa aaagcaaaaa attattttag gagccacttc tgctttaact atcgttattt
    18121 taattctaag tttgtttttt gtcatccgat catctacaaa aaaagaacca atcattcctg
    18181 aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga taagcaacaa ctagttactc
    18241 aagcaaaaaa tctggctcaa agctacgatt atgaccaagc gattgcgtta ttggaaaaag
    18301 atagtaagaa cggatctact gaaacacaac agcttcttca aacactaaaa aaggagaaag
    18361 aacaactgat cgactggtct gatcctactc agatctctca tgtctttttc cacagcttga
    18421 tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca aggatacaaa gattatatgg
    18481 tgactgttga agaattcaac cgttcgatcg aacaacttta tcaaaatgat tttgttttag
    18541 ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa tctttccttt aacgggcttg
    18601 cgctacctga aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc tattatgaat
    18661 atatggataa ttccggtttt cctagccgat tggtcgtatc aaataacaat gaaatcaaaa
    18721 atgtctataa agagaaagat aaagaagaaa tcggtgactt tgacatggtt cctctgatcg
    18781 atgcctttgt caagaaacat cctgattttt cttatcgagg cgcaaaaggt accttggccc
    18841 tgacaggtta caacggagtt ttaggttatc gaacatcaaa atctcaatat ggagacaatg
    18901 aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc ggaacagttg aagaaagacg
    18961 gctggagctt tgcttcccat acatggggtc acttgaatat gactcaagct tcactagaag
    19021 atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc acctatccta ggaaaaacaa
    19081 atattctgat ttatccattt ggtgcagaca tcagcgactg gcagccttat tcagcagaaa
    19141 atcaaaaatt tgcttacttg aagagtcaag gtttcgatat tttctgtaat gtcgatgctt
    19201 ctacaccagc ttggggacaa ttaggtacgg atttctatcg aaatgctcgg atcaatatcg
    19261 atggcattcg ttttcaatct gatttagacg gaaaaaatcc tattcttgat ccattcatca
    19321 atgtccatga tgtctatgac aaagaagcac gcgaatctgc ttgattttac gtcatttatg
    19381 aaccaaaaaa gccgaactga cattttatcg tcagtttcgg cttttttatg ctttatttct
    19441 tcttcggtgc gattcccttg atcaggactt ctgtccattc tgctgtatat ggaagcagtt
    19501 ttgcgtatat ctccggctta gaaattcctt ctaccattgc ttgaaaatag aggatcaaga
    19561 actcatccga atatttcaaa tccacttttc cttctttccg ccctcgatgg aaaagatcaa
    19621 gcatgatact gaaactttct tgcgtatacg catccatcgc gtcagaaaat tctttttttt
    19681 ctttttttgt atagtatgtc atcaaatcga tataaaattg tttgttcact cgattgagat
    19741 aacggatttt gttttcactc atggcaatca gtgtttcttc aaatggaaga ttttccgcaa
    19801 tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc aaaaaatact tcataaatca
    19861 gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga gcgttctcag
    19921 ctaccttatc cattgtcact ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg
    19981 cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc aaaaccgttc atttgtagag
    20041 cctcccgtaa ttttaaactt ttataatata tatagttcaa atttattctg aggtttatta
    20101 taactatcta ttgagaaagt atcaagctag caattccttg tttatgaatt cattgacaga
    20161 aatttataaa aagaatatta tgtactaaaa taataaaaaa agttttataa aaaggtataa
    20221 aaccttttta ttaaactatt taatcttgtt tagtatatat ttttagaaaa aggagtgggg
    20281 tttttatggc agaaatcgtc aaagtcacac atttgaaaaa gagctttggt cgtttcgaag
    20341 ctttaaaaga tgtctctttc accgtacatt caggtgaagt cgttggattt atcggaccaa
    20401 atggttctgg aaaatccacg acgattcgga cacttttagg gattataaaa aaagacggcg
    20461 gaacggcaac gatttttggg caagatgtat gggaagatag tttatctatc catgaacgtc
    20521 tttcttatgt tccaggcgat atctctttgt ggggaaattt gactggagga gcgatcatcg
    20581 atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac
    20641 gtttctctct tgatcctaag aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag
    20701 tgggcttgat tgctgctttg gctgttgatt cggatctcta tttatttgat gagcctactt
    20761 ctggtttaga tcctttgatg gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac
    20821 agggcaaatc catcctactt tcttctcata ttctcagtga agtcgagcga ctagcggata
    20881 aagtcgtgat catacgcgaa ggaacactcg atgaagaagg aacactcgat gaattgcgtc
    20941 atttgactcg ttcgaccatc actatagaaa cagaagcaga gaatagtcac ttagcacaat
    21001 tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg
    21061 aagctttcga tgtgatttta gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta
    21121 ttccaccgac tcttgaggat ttattcatga gacattatga aggatgagga ggaagcgaaa
    21181 tgactgataa attttcacgc tggtccaccc ttttttctca aaaccttagc cgtgactgga
    21241 aaaagatcct gatatggcta gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt
    21301 ttgaagaatt gacttccgga caaggattag ctggattata cgaaaccttg caaaacccgg
    21361 caatgattgc aatggtcgga ccaactccag cttcgaatgc tgctgagtat actttaggag
    21421 cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt tgctgctgtg atctctgttt
    21481 tacacgtggt gagccaaacg agaaaaaaag aagaacttgg tctgattgaa cttattcgtt
    21541 cctttcgagt cggacgtcaa gcaaactctt tagctgtcat cgctgaaagt attctgatca
    21601 atttgctttt agcaatatcg atcagctgta cgatgatttt tttccaagcg gattcgatca
    21661 ctgcagaagg tgcttggctt tttgggctat ctgtaggtag tatggggatt ttggggagtg
    21721 ccgttgcact gtttgctgca caactgatgc caagttcagc cagttcgaat ggtctgtctt
    21781 tagcgttttt aggtggactt taccttctac gagcaataac agatgtagct gattcctccc
    21841 tttcgatgtg gaatccttta ggctggtctt atttgaccta tccatttaca actaacaact
    21901 ggattccttt gctttatttg tttttgttca gtctaatctt gataatcagt tcatttgttt
    21961 tagaggaaca tcgagatttg aacaccggct atatccccga acgtgaagga aaaccgtatg
    22021 ctaaacgttc tcttttatcc ttgcgtggtt tattattcta tctaaataaa gggatgacaa
    22081 tcagttggct tatcactttt ggtttgatgg gggcggcata cggctctatc tacgggaata
    22141 tgcaacagtt tttagaaagt aacgacttaa tgaaacagat gtttatccaa tctggcgtct
    22201 caattgaaaa aaactttaca agcacgatca tggtagtctt gatttgtcta gctgctattt
    22261 taccaattgc tagcatcaat cgtttattta cagaagaaac ccacggacgt atgaatcagc
    22321 tgttcgcaac aaaaatttct aggaaccaac tttattggac caatatgtta ctagctattc
    22381 tatttgctat attggcaatc ctgattagtt ctggtgcact agggctttct gctttggctt
    22441 cgatgaagga tgcgaaagac atggatctat tgatgtttct taaagccggg ttcaatttcc
    22501 tgcctgccgt tttagtgttt atcggaatta gtggctttct attcggatgg cttcctcgaa
    22561 tcggtaaact tgcttatatc tacttagctt actcctttgc actcaattac tttggcggaa
    22621 ttttggattt accagaatgg ttttccaaaa cagctgtccc tagctgggtt ccacggatgc
    22681 caaacgattc ttttgatctt tccgtgttcc ttacgttatc atgtatcagc cttgcactgt
    22741 tcgtccttgg ttctattggt tataggcgaa gagacttact agaaggataa taaaaaagca
    22801 tgtacaattt catgctagcc tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt
    22861 ttttatttac aagtcttcta actgctcttt atcaatgatc cagacttctc gattctttct
    22921 ttttacataa ccattctcct ctaataattt aaattttcta gataaagttt cgggggtcgt
    22981 accgataaat gcagcaagtt ctttcatttt cattggcagg aaaaagtgat ccttccctgt
    23041 cgctttagct aagtcaagca gatagacagc gactcgttct tctacttttt ccatcatcaa
    23101 aaaagttgtc tgctgctgta cttttgttaa tttctcaacg ttgattgcta aaagtttcag
    23161 actaagggcg ggataatgca gtaagagttc cgagaaatct ttttgtctca atatgcaaat
    23221 ttccgttttt tctagtgctt cgccaaataa agactcattt ggaacgccta gcaactggct
    23281 ctctccctca tatccgcccg gctctattac tcgtaaaagc tgttcctttc cgctagcaga
    23341 taattgatac actttcatac tgccggaggc cacgatcact aattgaggtt ctccattcgg
    23401 cgcaagaatc tgttctcctt tttccacata gtgatgtttg acaagtgcgt gtactttttc
    23461 ttgatcttgc tgtactaagt gattgaacaa aggaacaaat gaaacacata aatgatcatg
    23521 actcataatc tctcctcctt ctaaacagct atttctaata aagaaacata acttccctta
    23581 ttccaactga taattttctt ttatcatagc ataaacgtca agcgaaccag ataccagcaa
    23641 atagagtgga tttctttttg tcccttgctt tattttcctt cttttgagac cttaagtaat
    23701 tgattcctat caatttttta aatatttact ttttgaataa tcaaaaacaa accttcactc
    23761 gcaagccgag agtaaggttt gtttttcgca ttgcttattt cgtgttgtct attgaaattt
    23821 ttccgcacct agcttttgat atagtggtgc tagttcttgt aaggtttcag cggttatttg
    23881 ctcaacgtgt tcgatttctt gcggatagaa gaatagttta cccatttgaa aggttcggtc
    23941 tctcatcgct tttttcaact cttcttgttt ctttgtatcg gacaaatagt cagacaaagc
    24001 taagtggtcg tcaagttcat gttctggttg cggccatatg taataatcct tgatatcgac
    24061 tgttttgctc cactctttca gtatcgcaag caactggtta tacgctgcaa tgctaccata
    24121 tcgttcttcg cttttataat gttggtacat caaatagatt tggtattgtt tttgattcaa
    24181 caaaactgct agacaggcat tctcgtcttg acgagattcg cttcggtacg cgctccaaaa
    24241 atgactgcgt aaattccagc cattcgtcca gctttcgatt ttaggtttgc tcatgccgta
    24301 catatcaggc aaaagtgcag ctgtctggag ctgaatctct ttccattttt gccagtgttc
    24361 tttatattct ttcttgattt gttcaatctt atctgttgga tggctctctt tgagctgttt
    24421 gaatgcgtag attggtcgat caaacaactg ataatctgat tttccaagtt ccattgtttc
    24481 gcctctttcc gttatctttt gtattctacc tgaattttgc gatatggaca aatagaggtt
    24541 gtgaaaaagc tgttctgcat caagtcataa gaacagccaa acaaaaaact gcgatgagca
    24601 tcaccgcagt tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa
    24661 atggcactag aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa
    24721 gctcgtcctt ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct
    24781 tttgttaaat tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt
    24841 ttatcgtcag cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact
    24901 tcgcctaatt tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc
    24961 atgaatgcag atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata
    25021 gagaatactt tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca
    25081 taagctgaaa cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct
    25141 agatcactga gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct
    25201 tggatttctt gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat
    25261 gaaatcgtct gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc
    25321 agtgttagag aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa
    25381 actacattga cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt
    25441 tccatcaaag gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc
    25501 gagtaatcat ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc
    25561 ttttctcgac gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct
    25621 cctagttcga atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg
    25681 atcgtcttag ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca
    25741 ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt
    25801 tttccagcag tatacgtact attttcaacg gcgatatcat catctacgat cacactttct
    25861 acttcaatat cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct
    25921 gtataattct taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc
    25981 tgaatctgat ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc
    26041 ccttccccta caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa
    26101 ccaacttgtt ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca
    26161 agttctggat agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca
    26221 ttcatgattt ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa
    26281 gatttgctga gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac
    26341 taaaggcgca tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc
    26401 cagttccgag ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc
    26461 gcttgattca ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat
    26521 tccgccgaga gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt
    26581 aataccatct gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat
    26641 cttcctttct tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa
    26701 gtatataata aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata
    26761 ctttaattaa aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga
    26821 aaagccgtag tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca
    26881 tttaaagact tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa
    26941 aacgaactaa tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca
    27001 ttaggagctg atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat
    27061 ggcatgctga tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc
    27121 tgttttaata actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag
    27181 tctaacttcg aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat
    27241 tccgtaagct gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt
    27301 tttttcattt gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg
    27361 aactgtttct gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc
    27421 ttttattcgt tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac
    27481 tgtttgtgct tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg
    27541 agtttgactt agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc
    27601 ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc
    27661 ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc
    27721 taaatcttga tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc
    27781 ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga
    27841 ggtagaacta tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt
    27901 cggtacattg gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat
    27961 ggttgttgaa ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa
    28021 taagaaaata ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac
    28081 ttttcccatc cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga
    28141 accataaaca caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt
    28201 tccgggcttt tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa
    28261 gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa
    28321 agaaggtatt tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa
    28381 aatcctgcga ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc
    28441 tgttcgtagt ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa
    28501 atacaaaaga atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt
    28561 ccaatatgtt taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt
    28621 ccaccttttg cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc
    28681 atgaaaaaga taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa
    28741 gcaatactga tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct
    28801 tcttttcgat acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac
    28861 tgtgttcgtt taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc
    28921 aatttggtac gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca
    28981 gggcgaatga aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca
    29041 tcattgctta aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct
    29101 tttcctttat ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta
    29161 gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg
    29221 tgataattca atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta
    29281 tgggctggca ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct
    29341 ttttgcttat gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg
    29401 cctaaaatcg gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat
    29461 aaattacctg tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat
    29521 ctgcaatgaa tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt
    29581 ctctctcttc ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt
    29641 ttttcaattt ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat
    29701 tgttccacct agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa
    29761 agccgattaa gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct
    29821 tggctctttc tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca
    29881 tccatcctaa aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa
    29941 aatacttatc tttcaccaaa attga
//
LOCUS       DASJTM010000031        28296 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJTM010000031 DASJTM010000000
VERSION     DASJTM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.031"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(448..618)
                     /locus_tag="U4B29_001254"
     CDS             complement(448..618)
                     /locus_tag="U4B29_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2106214.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(812..991)
                     /locus_tag="U4B29_001255"
     CDS             complement(812..991)
                     /locus_tag="U4B29_001255"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106215.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1378..2490
                     /locus_tag="U4B29_001256"
     CDS             1378..2490
                     /locus_tag="U4B29_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2106216.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2568..2861)
                     /locus_tag="U4B29_001257"
     CDS             complement(2568..2861)
                     /locus_tag="U4B29_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN2106217.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3029..3343
                     /locus_tag="U4B29_001258"
     CDS             3029..3343
                     /locus_tag="U4B29_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN2106218.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3364..5343
                     /locus_tag="U4B29_001259"
     CDS             3364..5343
                     /locus_tag="U4B29_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2106219.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5356..5682
                     /locus_tag="U4B29_001260"
     CDS             5356..5682
                     /locus_tag="U4B29_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN2106220.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5698..6216
                     /locus_tag="U4B29_001261"
     CDS             5698..6216
                     /locus_tag="U4B29_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106221.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6216..7568
                     /gene="celB"
                     /locus_tag="U4B29_001262"
     CDS             6216..7568
                     /gene="celB"
                     /locus_tag="U4B29_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2106222.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7652..9157)
                     /locus_tag="U4B29_001263"
     CDS             complement(7652..9157)
                     /locus_tag="U4B29_001263"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN2106223.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9249..9839)
                     /locus_tag="U4B29_001264"
     CDS             complement(9249..9839)
                     /locus_tag="U4B29_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2106224.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10018..11664
                     /locus_tag="U4B29_001265"
     CDS             10018..11664
                     /locus_tag="U4B29_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2106225.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11713..12432
                     /locus_tag="U4B29_001266"
     CDS             11713..12432
                     /locus_tag="U4B29_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2106226.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            12911..13858
                     /locus_tag="U4B29_001267"
     CDS             12911..13858
                     /locus_tag="U4B29_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2106227.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            13870..14790
                     /locus_tag="U4B29_001268"
     CDS             13870..14790
                     /locus_tag="U4B29_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2106228.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            14831..16288
                     /locus_tag="U4B29_001269"
     CDS             14831..16288
                     /locus_tag="U4B29_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2106229.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16633..17247
                     /locus_tag="U4B29_001270"
     CDS             16633..17247
                     /locus_tag="U4B29_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2106230.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17240..18964
                     /locus_tag="U4B29_001271"
     CDS             17240..18964
                     /locus_tag="U4B29_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2106231.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            18961..20412
                     /locus_tag="U4B29_001272"
     CDS             18961..20412
                     /locus_tag="U4B29_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2106232.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(20885..23038)
                     /locus_tag="U4B29_001273"
     CDS             complement(20885..23038)
                     /locus_tag="U4B29_001273"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN2106233.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23040..24086)
                     /locus_tag="U4B29_001274"
     CDS             complement(23040..24086)
                     /locus_tag="U4B29_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2106234.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24236..25525
                     /locus_tag="U4B29_001275"
     CDS             24236..25525
                     /locus_tag="U4B29_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN2106235.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            25538..28240
                     /locus_tag="U4B29_001276"
     CDS             25538..28240
                     /locus_tag="U4B29_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2106236.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9191 a         4880 c         5687 g         8538 t
ORIGIN      
        1 tttagaaaga aacgaaatat atgagctaaa ataaaaaatt gaataagcat agtttttaaa
       61 atcaactagt acccatcaat tgaatggaaa ctaccgattt tagaaggggt atacaaacaa
      121 atattagtat cggagtagag aaggttgaac ccaaccagta aataagtaaa gacaattttg
      181 tgctgcgttg agaagtgaaa aataaacctt tgtagtaata ctagttaata aaaaatatac
      241 aaaaacactg cgaaaatcat tcttgagttt cgcagtgttt tttgcctaat gactcaagtt
      301 tattgggtaa tactttgatt ttctttctac aaataatcag cgatcagttg taattttttc
      361 tctagtttta atggctcttt gtcaataagg agaaaaacaa gtcgggtctt ttgtctcgga
      421 aagcttcaac caactcgtga ataaaggtta cgcctccttg aaaggcgtgg aaaaaccaag
      481 taatcgatca atcaccaatt tcgcatttgg tagaacatgt ttggtgagct gaaagtaggt
      541 ggcgttcatg tctgtaacta agaattctat ttctttagga ttagtgcagc taaggaaata
      601 gtttgttaat cgaggaattt tttgtgtagg taaattttct cgattaattt ttctgtctcg
      661 ccatccgcgg aaataaaact cagtttatcc tcaacggaaa cgtgagaacg aaattcacaa
      721 ccctcaacac tcaagacaga attcttttgg aagactttgg taaatagctt ctcaactctt
      781 tcaatgtgcg aataacggtt gtcaaagata cctaacaact tttcgaaata aaaaataacg
      841 acactttttc agtcagtaaa gaagtgattt tatgtctgac atgatttgaa atcgagtgcc
      901 ttggacggat aaaataactt tgagcgttcc aatggcttcg acagttttta caggtatagc
      961 gctgcttttt taggcgtata accattggca tatgattata ttattcaaag tggacaatgg
     1021 tttccttctt cttgtttttc accaccatta atttcctatt tccatctacc acagcagaac
     1081 cacaatttct acagacacat ggagcaggga agagaactgc agcaacgatc aatgtttttt
     1141 tcttctgtaa ggtttcgtaa gagacctctg taatcatcaa atttttgtct attgatctca
     1201 gaattttttt gatagaacca ttcataaagc atatcgttct ctcagttgtt tattttgtgg
     1261 tgatttaatc atacaagaaa acgatatgtt tttttataaa aacatgaata ggaaaaatag
     1321 ttgacaagaa aatgacaacg ccatatgatg ttccatagta tttacaacct gtcatagatg
     1381 actgtaaaaa ataaaagtaa aaagaaagga cgacaacagg tggattttta tcagtctcta
     1441 caactagatc cgtttatttt aaaacaaaaa atacgagaag cagcttctaa aaaagaaaag
     1501 tgcatgtata tttgttcatt gtttttacga tcattattca tcgtattgtt tgccatttgt
     1561 tttatcatta taattacaac attatttgaa tcgaagcata aaccatacgc tgtggtttta
     1621 ttctgtatgt tgatgagtat tcgttttgtt gactttggtt ataagatttc ccattcgatc
     1681 ataggtttag caatcgtcat gttctcacta ttagtagcac cgtatgtcca gctgatcaaa
     1741 tggtcagtta tgggcatgtt gattcatttt attttactaa gcagcatatt aatggcaacc
     1801 gctagtgatc ccaaaatggg gaatgcttct ttgtacggct tttcatatct ctttattgtt
     1861 tattcgttac ctaaagattc gttgaacaag aatttcttta cacaaacaag tagcttatta
     1921 tttttgtttt tttgttggtt ttcagttctc ctttacagaa aacatcgaga aaagaacaaa
     1981 gacaaaagct tgtttaaaga gatttttttg aaaggcatgt attcgcagga aaaaatttgg
     2041 atgctcatct atgcatttgg cattagtctt ttgatcgttg caggagagta tgtccctttt
     2101 cagcgattga tgtgggcagg gtttgcattt tcttctattg tatctagcta tggattgatg
     2161 tcgattggtt tcaaagaacg agcagtagac cgaatcattg gctcattgat tggatgtgtt
     2221 ttatttattg gtatcagcca atttattcct tttaactggg tcggtatatt aggtggtcta
     2281 gctttaggtg tttgttctac ctatagatat aaaacagttt ttaattcttt tggtgctttg
     2341 gcaatcactg ccagtttatt cggggttcca ggagcagtaa cgatccgcat ttttgagaat
     2401 atccttgggg tatgtttagg aatcatgtat attggggtga ctgaaatact tatccgaaaa
     2461 ataagagaaa aacacggttt gaatcattag atgagatcga ataaaaaata aggaaaaagt
     2521 gaagatacac atggatgata aattgctatc attccatgtg tatcttttta cggtaaatca
     2581 gtgaaacgtt cgactttcat ttttaattta tactttttac ggagttcgtt tatttgagcc
     2641 atcatttcgg atttgtgatg cttttcgatt gcttcttcat ctttccaacg gtcgatcagt
     2701 aaaagagatt ctggatcttc tagtggaaag taatagtcat atttctcgtt tccttcttct
     2761 gcacgaatcg ctttgacaat gtttctttcg accatttctt ctgcaaattt tctggcttgt
     2821 ccatttgtac ttgtatacaa aatattcact gtgatactca ctaaatcccc tcctgaaaaa
     2881 attctatcac tagtatatca cggtgccaga aattattatt caagatatga tacacatttt
     2941 ttccgcaatg ctacggaaat atatattttg aatagaaaac gcttacaaga tatgcttggt
     3001 ttataaaaat aaaaggagtg attgatttat gaaaaaagca ttgattattt gtgcggcagg
     3061 aatgtcatcc tcgatgatgg cctcaaagac aacggatttc tttaagcaaa aaagagaaga
     3121 ggtactcgta gatgcagttt cagctacaga aggcgataat atgattaaga atagtgattt
     3181 tgatttattt ctgatcagcc cgcaaacgac gatgtttttg gataaattca aaaaattagg
     3241 agaatctgtt ggaaagcctg ttgttagtat ccctttccaa gcgtatgtcc caatccctac
     3301 aggcatccag aagttagcag aggtgatcga agagaacata tgatggaata ggagtggatg
     3361 ataatgaaac agaaggaaaa attgctgttg agatatttga ttgatcacaa gaaggagtat
     3421 gtcaccagtc aacgtttagc ttctgaattg ttattatctg atcggacaat ccgtaattac
     3481 ttgcagcgga tcaaagagtt agtagaaaaa aacggaggta agatcattgc taaaccaggt
     3541 tatggctacc aactgcatat tttacaaaga ctgacatttg acttgttttt gagtcggcaa
     3601 gaaatagtac ctggatatgc tagagaaccg caaggatttt atgagtccga agatcgacaa
     3661 aagtatatat tgaaccaatt gcttttggaa gatgccattc tttttatgga tgatttggca
     3721 gaagagcttt atatcagtcg ttccagtttg acaaaggaca tgcaagaaat caaggagcga
     3781 ttgattccat attcactaaa aatcgtttct aagcatggtc aagggacatg gatcgaaggg
     3841 gaagaaagaa atcgacggca ttttatcatg gatacttttt ttggaaacca ttatggaaat
     3901 tcattgaaag aatatttagg gcaaagccgt tttttcccag acatcagttt tgaagagcta
     3961 gtcattatca ttctcgatga aacaagagaa gctaagttaa aagtttcgga ttttatcata
     4021 cagaatctga ctttacactt ggctttgggg atcaaacggc tccgagaagg gtttgaaatc
     4081 aaggaattag gcatcgaaca agaagtttct aagcgagtag agtatcaagt ggcgcagcgg
     4141 atcgttcaac gtattgaggt aatcgcaggt gtatcgtttc ccgaagaaga aattgcttat
     4201 ttgacattac atctcatggc taagtcgaat cacaaagaaa atcaaaatga tcaggaacta
     4261 tcaaaggaat tagcgaaagt tctgaaaaga ttgtctcagg aatttcattt ttcattagta
     4321 gaggatctcc agcttttcaa tggactactc gatcatttgt caccgatgat gattcgattg
     4381 agccgaggta ttcgcattga gaacccattg actgaggaga taaagaaaga aaatcccaaa
     4441 gtctttgacg cagtaaaaag gcatttcagc aacatgccgg ctttgaaaaa ctataccatc
     4501 aatgaagatg aatgggccta tttggctttg catctcatgg cggcattgga aaaagaaaga
     4561 gctgcgcata aactacatgc attgattatc tgtgcaacgg gttacggcag tgcccaacta
     4621 ttaaagaatc gcgtagtcag tgaattcggt aaaaatatta cagtggtaag tgtaaaaggc
     4681 tattatgaga tcaatgaaac aactttaaaa ggaatcgacc tgattatttc ttctattgat
     4741 ctttcgacaa tgtttttcaa aattccagtt ttacatgtca gtattttttt gaatgaagat
     4801 gatgttcgta aaatacgaaa ggtagttgga gaatctatac cctatcattc ctcagataga
     4861 ccgataccag cattttctct acagcaaaaa aagcagattt acaccgaaca gttgtctgaa
     4921 aaatttttca agacttatac gtacgaaccg aaaaaggaag aaattcttca tgatttgctg
     4981 caaaagctga gcataaatga agaagaagga tatataagag aaatgcgaaa gcagatccag
     5041 caacgtgaaa atatgggaca aatcatattc agtgatacag tggcagtccc acatcccgct
     5101 gttccagttg gaatcgaaac aaaggtagcc gttgcattga ttccttctgg gatgcagtgg
     5161 gatcaatatc aagaaattca ttttgttttt ttagtttcgc cttcttatgt cgaaaatgaa
     5221 ggaattacgg tcgtgactaa agcaatcgtt aggttagttg accagctaaa agtacagcag
     5281 gaaatcttag ctgaacctat ttttgagaat ttcagcagta aatttatcaa actgatcgaa
     5341 taaggaggaa aacagatggc tgaaaaaata aacagtgaaa cactacaagt gacttctttt
     5401 gatatcattt tccatagcgg aaatgcacgg acactgatac atgaagcatt tcgctcgatg
     5461 cgtaaagaga tgtttgaaga agtagaagag aagttgcgac aagcaaatga agagattctt
     5521 catgcgcatc agtcacaaac tcaacttctg aaagaatatg ccagcgggac aaaaatagaa
     5581 atggaaatta tcatggtcca tgcacaagat catctgatga cgacgatgac attattggaa
     5641 gttgcacagg aaatgaatta tttgtatcaa aaaaataaat agaaaggaag gatcattgtg
     5701 caggatacag taaaaaggaa aatcgaatta gaaaaaaagc aacttagttt gaaaaacatg
     5761 tatttcaatc gttatctaat cgtacgttac ctgactgcct ttttcttttt catgaacatg
     5821 caatggatga ttctgcttct atcagctaaa agtttaggaa gtagtttacc gatgattctg
     5881 attttagcta ttttgcctgc agtaggagaa caagtaaagc tttatcgaaa acaccaaacg
     5941 aacgttccat ggacaaagtg gtattttttg ttccagagtg tctttaattt attagtgatt
     6001 ccagtgttgt ttacctcagg atttacattc ttgtatcctt ttatggcaaa taacaatgga
     6061 ggacagttat ttgttttcat tcttgttgta agcggtattt ttgccagtgc atttatacag
     6121 tatcgtttaa agaaaatttc actcaaccaa gaccaacagt atatccgtat taaacaatat
     6181 gaaaaagcgt tatatttagg aaaggagaat aactaatgtt tgattttttg cagaaatacc
     6241 tgatgggacc tatggggaaa attgcgcaac ttaaaatcgt tcgggctgtc atggcagcgg
     6301 ggatggcttc tattcctttc acgattgtag gctcgatgtt tttggtttta aatattttgc
     6361 cattaccgtt cccattttta gaaggatttt ttaatgcaac ttttttcaaa gttagtgact
     6421 tgtacatgat cgtcaataca atgacgatgg ggattttatc tatttacttt gccattgtgt
     6481 ttgcttatga attaacgtca attgaacgag atgaacaaaa attaaatgtt aatccgctca
     6541 cgggggcatt actatctgtt tttgcattct ttatgtgtat ccctgaattg atcatgactg
     6601 atggtaaaat cagcttgatt gcttctatca atgagacaga aactgtagta aatggtatta
     6661 ggatgggctc ttttgtcgaa cgtttaggaa cttcaggaat ctttacagct attattatgt
     6721 cgattattgc ggtcgaattg tattgtatgt gtgtcaagag aaattgggtc ataaaaatgc
     6781 cagatgttgt tccgccagga gtttctcgtt ctttcactgc tctgatccca acatttgtca
     6841 ttgcgtttgt ggtgatgatc atcaatggat tattagttgc actaggaacg gatatcttca
     6901 aaatgattgc tatcccattt gggtttgtta cgcagttgac gaatacatgg cttggtgtca
     6961 tggtgattta tttcttgatc catgcattgt ggatcgtagg gatccatgga gcgaatatca
     7021 tcacttcttt cttgaccccg atcgtattag ctaatatggc agctaacgca caaggcgcaa
     7081 attatccgtt ggcaggagaa ttcaacaatt cttatgtaac cgttggtgga tcaggtgcga
     7141 ctttaggatt gatcatcttt attgcattcc tcgcgaaatc tgatcagctg aaagtgttag
     7201 gaaaagcctc acttgtacca ggattattca atatcaatga accaatcatt ttcgggatgc
     7261 caatcgtcta caatccctat ttagcggttc cgtttttctt agctccgatg gcttctgcta
     7321 cactcgctta ttttgccatt cggctggaaa tcgtcaaacc gatgcttgca cagatgccgt
     7381 ggccttcacc agtcgggatc ggagcatttg ttggtagtgg tggtgattgg aaagctgctg
     7441 tgttagcagt catttgtgca atcatcgcat tcattatctg gttcccattt atcaaattct
     7501 atgataataa attattaaat gaagaaagag aaaaggcagc agaattagct agtgaaaagt
     7561 taaaatgatt tctaagaaaa aaggaactgt gacaaaaaat cacagtccct ttttttctta
     7621 gaaatgggaa accactagta tataaatacc attattcttc aatcaaccgc ttcacgattg
     7681 cataatcccc ttcataaggt tcatctacac cattgatttt ttgataggga tccacgcctt
     7741 ttcccgcaat aacgactgaa tcttgcggag cgctttctgc taaagcgcgt cgaatcgctt
     7801 caggacgatc catttcataa tgaacgatca aatctggatt agtaattgct tcattgattt
     7861 cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc tgccgtcaaa aaggcgatgt
     7921 cagctgtttc tgatagcact tttccaaaat catgtcgacg ggagattgct ttgtttcctg
     7981 ggctgcctaa tacaacaatt acacggccat ctggatgttc atttttggca aatttcagca
     8041 atgttttcaa gcttagataa ttatgtgcat agtccacata gacatgcgct ccattggatt
     8101 ggatcaattg atccattcgg cctggtacac gagcttcttt taatccgtct tgtgcatctt
     8161 gactggaggc acctgttagt gctgcggcaa tgatcgcact ggctgcattt tcttggttga
     8221 atcctccagc cagcaaaata tcataagcag tatcgcagat agctaagcga tcttcatgag
     8281 aagataaagt aaaaccgtga gtacctttat ctgaacggat cacttgataa ttgctttctt
     8341 ccgaacgacc ataagtgatc gttggaatct ggaatagatc ggctgtttcc ctaagtaaat
     8401 caaaatagtc actttcatga tttaaaacaa ctgtttttga attcaagatc aattgtcgct
     8461 tgcaatagaa atagtcatca aatgtcggat gttcgatcgg actgatatgg tcaggcgaga
     8521 tattcaagaa gattccgaca tctaaagtca agccgtatac gcgttgtgtt ttgtaagctt
     8581 gtgaagaaac ttccatcact agatgagtca taccattttc aactgcttct gacatcatac
     8641 gatataaatc taatgactca ggcgttgtca aatgggattt gaaatatgtt ttgccatcta
     8701 acgttgtatt catagtagaa agcaatgctg tttttttatt tgttgtgtga tccaagattg
     8761 cttttgtaaa gtacgcagca gttgttttac cttttgttcc agtgaatccg atcactttca
     8821 atttattttg cggatagtcg taaaaagcca tagcaagaag cgccattgct ttacggatat
     8881 ccgttacgat gattcctact gctgaatttt cgtatggttg ttctgataca taatattgaa
     8941 taccctgttc gattgctgaa tccaaatatt cttctttgaa attcattcct ttacagaaaa
     9001 acaaagtaga agcatcagct tttcttgaat cataacttaa ctgaagaaat tcagcttttt
     9061 ctggtacatc aagatgccag cctcttgttg aaacaaattc ttttaataga ttttctttta
     9121 atagtaaatg acggatgtct tctaaagaga agctcattga ttgatccctc gctttattca
     9181 atttcttggt ttattatagc acactctttt taatatatcg tttgtttccg ctatgacaaa
     9241 atccagcttt attcaaattt tcttgacaca ggggacaagc ggatgagtat tttcataaac
     9301 aatgaactga ctgtaataga taggagaacg aatagtgtct ccaacaatcc aaaaacataa
     9361 taattcaaaa agacgataag actattggta agtaaaagtc cttgcggcat tggaagccga
     9421 aaaaatttgt ggaaaataat cggcggtatc gttataccgc catttgaggc cccgactgtg
     9481 tacaatgtgt aaaccccaag actgaacaaa agacttcctg aaatcagtga aaagaaatgt
     9541 gaggagatca tttccacttt tggaacaatc tctaaaacaa caggaaaaag cagacttccc
     9601 gctacagtct taaaaaatac tgattttcct aataggacag cggctagaag cagcatcata
     9661 agattgatgc tccaaacagt aactgagcgt tggatagaaa aggccgtttc aactaaatgt
     9721 ccgatggcgc ccactccggc ggaagctata tcgtgaggga gcaagaacca attcaatcca
     9781 actgtgataa agaaaagacc taaaaatata acgggtagtt tttttatttc ttgtgtcatt
     9841 gatatctcct ataaatgtta gccataaaag gctgtatatt tctttacttt accaaaaaaa
     9901 cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt atataaaatc gattgccagt
     9961 ttctgtttta ccaattaaaa tgatagtaat gttttgtaga aagttggaat ctgaccgatg
    10021 gataatcaaa gaaaaccagc attagatcaa ttaacaccgc aagaaaaaat ggcacgaggc
    10081 tctgcttgga tgaccgccag taatatcatt tcaagattgc taggcgctgt ctatatcatt
    10141 ccctggtatg cttggatggg cgaaaatgct aaggctgcca atggattatt caatatggga
    10201 tacaatatat atgcattgtt tctactgatc tctacagcag gaatacctgc tgcgattgca
    10261 aaacaaactg cacgctataa ttcattgaat gaatatggca cttcacgacg attatttatc
    10321 cgtgcattac agatgatggg cgggttaggc ctgttgtttg ctttgttcat gtacatcgcc
    10381 tctccttggc tagctcatgc ttctggtggc ggagaagaac tgattccaac gatgcgttct
    10441 ttgagtatcg ctgtattggt ttttccttgt atgagtgtga tacgagggta tttgcaaggc
    10501 aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag aacaggtggc acgtattttt
    10561 tatatgctat tggctacatt catcatcatg aaagtcatgg atggtgacta tgtgacggcg
    10621 gtcacgcaat caactttcgc ggcatttatc ggtatgctag caagtattct tgtccttttg
    10681 tattttttga agaaacatca agcgtataca tcggcgttta ttcactacag cgaaaacaaa
    10741 ataaatattg caacgaagga attgttgtta gataccgtta aagaagcgat tccgtttatt
    10801 attgtgggtt caggggtaac gatcttcaaa ttagtcgatc agttcacttt tatgaagatc
    10861 atgtctgcat ccaccgagta ttccaatgca cagttactgg atcttttttc gatttttagt
    10921 gccaatccag ataagttgac gatggtagtt attgcattgg caacatcgat cgcgtctact
    10981 gggttaccgt tgattacaga agctgttacg ttgaaagatc gagtaggtct ggcaaaattg
    11041 acaagcggaa atttacagtt gttttcattt tttatgtttc cggcgacatt tggtgttatg
    11101 ttacttgcct atccgctaaa tacattattt tatacaccgg attctctagg aagtaatgta
    11161 ctgatccaag caagttttgt aggactcttc ctaggactat atatgctagt ttccaacatg
    11221 ctgcaaggaa tgtttgaaaa caaggcggca attaattatt tggttattgg gtttctagtg
    11281 aaactccttc ttcaataccc agccattcgt ttatttgaag tatacggccc tttattggcg
    11341 acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa agagaatcca tgaagtagcc
    11401 cgctttaaac caagttttgt ctggcgcagg acattgctga tctttattct taccttgatt
    11461 atgcttttgg cttcgtggat cacaaagatg atttttggtg cttttttaaa cgaagatagc
    11521 aaattccagt ctctattgtt gattcttgta gtagcaggta taggggcttt agtgtatggt
    11581 tatttagctt tgaaaatccg cttggcagaa agactactag gaccaggaat agcgcgttta
    11641 cgacataaat taaaaataaa ataagtgaat cagaagagct agattgatgc tcttttgaaa
    11701 tgggggaaac gcatgcgttt agataagttt ttagcagata tgggacttgg cagccgcaaa
    11761 gaagtcaaag gattaatcaa aaaaggaacg atcgaagtca atggacaagt ggtgaaaagc
    11821 gataaagcgc aagtaaatga attcgaagat gcggttactt atttaggaga aatcattacg
    11881 tatcaaaaag atttttacta cattttgcat aaacctgcag gagtcatttc tgctactcag
    11941 gataatcatg atcaaaccgt aatcgattta ttggaagacc aggattatcg ggaagatctt
    12001 tttccagtcg gccgattaga taaagacaca gagggcctct tgattttaac gaatgacggg
    12061 gtgtttgccc atcagttact atcacctaaa aaacatgtgg agaaagaata tctagctgag
    12121 gttagcggta taatgacaga agaagatatc cgtttatttg cagaaggact gattattgat
    12181 ggcgatgagc agacattgcc ggcaaaactc caaattgatc aagtagatca agagacagaa
    12241 acttcaatta tccgtctgat tttgcatgaa ggaaagtttc accaagttaa gcgaatggtc
    12301 aaagcagtag gaaaagaagt cttatattta aaaagaatcc gtatgggaaa attttggctg
    12361 cctaaagatt tgaataaagg gcaatatcga cccatgacca aagaagagct agaacaagtg
    12421 agagaaaaat aattgaatgg atagctaaaa gaataagcag agtagttgta gcgccatctg
    12481 aaaagcagac ttgtccgtct gactttcgga tggctttttt gtgaaatttc ggcttatttc
    12541 cgtagaagct acttctggaa caccgtttgt tcggaataag ggactgtgac aagacttttg
    12601 ttacagtccc cattgttcct tgtgactcag agttagatag taaggacacg atctccctaa
    12661 tactcaggcc aaaacgctga attcacaacc tctgataaac ggtgttcaat cagctgaccc
    12721 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttcccgttt ttttctctaa
    12781 tactcaggcc aaaacgctga tttcacaacc tctcctatac tttgtttcat tttccctata
    12841 ctttgtttat ttattctcat tattgaaagc gctataatta cgttgcgaat gaatcgaaag
    12901 gggtttttag atgaaaaaac gaaaggggtt caatcatttt tttatgaatc tatggcgtta
    12961 taaagcactt gtcctgatgg ctgtaccggg tatgctttgg atgatttttt tcttttacat
    13021 acctgtaatg gcgaatgtcg tagcattcaa aaattttcat atttcagcag atgggttctt
    13081 ggctagcttg agggaaagtc cttgggtggg ttgggaaaat ttcaaatttt tattttcttc
    13141 ggatcaagca tttctcatca ctaaaaatac aattttatat aatgtagcat ttatcctttt
    13201 gaatctgctg atctcagtcg tatttgcaat cattatgagc gagttgagaa ataaacgact
    13261 cgttaaagtt tatcaaacga tgtccttact accttatttt ctatcttggg tcattatcag
    13321 ttactttgtt tacgcttttt taagtcctga caaaggaata ttcaatcagt ggatcactgg
    13381 acatggagga gaagctgtca attggtataa tgaaccgaaa tattggccgt ttatcttggt
    13441 gttcattggt acatggaaag ggattggtta taacagcatt atttactttg catctgttat
    13501 gggaatcgat ccgacttatt atgaagctgc tatggttgat ggagctagca aatggcagca
    13561 gatcaaacat gtgacgatac cacaacttat tccactaatg acgattttaa cgattttagc
    13621 cgtaggaaac attttccgag ctgattttgg cctgttttat aacattccta ggaattccgg
    13681 tgcattatat gatgtgactc aagttttgga tacgtatatc tacaatggcc tgacatccac
    13741 aggagatttt ggtatgacgg ctgctgcagg actttaccaa tcagttgtcg gctttgtctt
    13801 gttgatgatc acaaatacga tcgcccgacg ttttgatagc gattctgcat tattctaagg
    13861 aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag tccgctcttt caataagacg
    13921 gctaattttt tctctaatat aatgattgca ctctttgcta tttcctgtat tttgccattt
    13981 atatttgtta tcgccatctc ttttaccgat gagtccatat tgtcgactca cgggtacagt
    14041 ttttggccag aaaagttcag tatatttggt tatacgtact tgttcgacca gatgcaggat
    14101 aaaattttcc aagcgctttt cgtcactgta cttgttactg ttttaggtac actgatcaat
    14161 agcacggcta cttctttata tgcttatgcg atttcacggt caaactttcc attccgtcgt
    14221 ttttttaccg ttttttgttt gattacgatg ttgttctcac cagggatggt ggccaattat
    14281 cttgttatga caaatcttct tcagttaaaa gatacgattt gggcattgat cttgccaatg
    14341 gctgtcagtc ccttcaatat tattgtgatg cgcacattct ttaaacgttc ggtctctgat
    14401 tcgattattg aatcggcacg gatcgatgga gctagcgaac tgcgaatatt tgttcagatc
    14461 gttttaccgt tagctgttcc aggtattgcg acaatcagtt tatttgccgc attgggttac
    14521 tggaatgact ggttcaatgc tttactatac atccaagatg ataagctagt tccactacag
    14581 tatcttttga tgaaaatcca aagtaatatt caatatctga cacaaaattc aggagcagga
    14641 agccaattga caggcggatt agcctcagtt ccaggagaat ctgccagaat ggcgatcgta
    14701 gtcatctcga cgttgcctat tgcgatcagc tatccatttt tccagaagca ttttgtcaaa
    14761 ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa atataaatca taaaattagg
    14821 aggaagaaaa atgaagttat ggaagaaact agcctttaca ggtgtttcag cgttgttgtt
    14881 gggaacatta gcagcatgcg gaggttctgg ttctaaagac caggcagaag ctagcaattc
    14941 ggacactttg cagatgtatc aaatcggaga taaaccagat aatttcgatc aattgatgga
    15001 agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa gtcaatatca attacattgg
    15061 ctggggcgat tacgaaaaga aaatgaacgt catcatttct tccggggaaa attatgatat
    15121 cgcctttgcg aacaactatg tttccaatgc ccaaaaagga gcctttgctg atttgactga
    15181 attagcgcct aagtacgctg aaaaagctta taatgattta gatgaagcat atattaaagg
    15241 aaatctggtc aatggaaaat tatatgcttt cccagtcaat ggaaatgtct ttgctcaaca
    15301 agtacttact tttaacaaag ctttactaga taaatacgat ctttcaattg atggaatcca
    15361 atcttacgct gatgcagaaa aagtcttgaa ggaattccat gaaaaagaac caaacacggc
    15421 agcttttgcc attggacaag gcttcaaagt ccaagggaac tttgattatc ctttagggaa
    15481 tacgttacca tttgctattg atctggatgg agatgattcg aaaatcatca accaatacga
    15541 taatgaaacg tatatcggac tgttacgtac aatgcatagc tggtacaaac aaggactcat
    15601 tccaagtgac gctgcaacga gcaataagga ttatccacta gaaggaaata cttggctaat
    15661 gcgtggagaa acacaaggac cgtatgatta tggcgataca attttgacaa atgcagcaaa
    15721 acaagaactt gtctcaaaag caattactgt tccactaaaa tctacagctc aagcacaaat
    15781 ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa aaatcagtag aacttctagg
    15841 gctgcttaac agtgatccag aattattgaa tgggcttgtt tgggggatcg agggagaagc
    15901 atgggaaaaa gtcgatggtt cagatgataa aatcaaattg ctagatggtt atcagccaaa
    15961 tacacatatg tctgcttgga atacaggaaa caacaagatt ctatatacac aagagtctat
    16021 tacggatgac atgattgcca aacgtgatca atcgattgct gatgcagaaa cttcaccgat
    16081 tttaggattc agcttcaaca ctgacagtgt caaaacagaa ttaagtaata tttctaatgt
    16141 catgaatcaa tatttagatg gattgaatac agggactgtt gatccagatg aaacattacc
    16201 gaaattgaaa gacgctttgg acaaggctgg ctatgataag gtattgaaag aaatgcaaaa
    16261 acaatatgat gagttccgac aagaataata atgaataaag acaaaggggt tgtgacaaaa
    16321 gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa aagccagttt ctcggtatta
    16381 agctaaatat atgtaaaaat gtggaaagcc atattcacaa aatttttctt actactcgaa
    16441 actaagcggc tatttcacaa actcttggta aacggtgttc aatcagctaa ccctcggtat
    16501 tcgctcacaa aacgacaaaa tatgaggaac tatttttccg tttgtttctc taataatcag
    16561 gtcaaggtgc tgattgccca accttttttt gtataatgag aaagtataaa cttaatgttt
    16621 ggaggagaag atatgttagg gaaggttttg gaagagttat ttatccggat ctgggtcatt
    16681 ataaaactaa ccttgtattt ctggatatat acatttgccg gcgggatcat ctttggatta
    16741 ggtgcggcat ggaaaacagt caatgaacta ttttatttgt acggattcga gtataaagaa
    16801 atcacaataa aaagaggctg gaatatatat aaaagaaatt ttttaagagg caatctgcta
    16861 tttagtttat ttttatctgg tactgcttta ttgagttaca atttatattt atctgttcaa
    16921 atcaaagggc tcattttttt agctattgat tttattttac tttttgcctt gctctgccta
    16981 tttgctactt atcaatacag tatgatttta gatagtgaat atacgatctc tgttcctaat
    17041 ctgctaaagc taagttttat ttcggttttt tccagctttt cgtcttttct aaaaataatt
    17101 attggtagcg gtataatttt aggcgtgact tggcaattca aaggattgat cctgtttggt
    17161 gtcattggtc ttttgacggt ttggaatggt acgatgacaa cacattggcg tgaagaatta
    17221 gataaacagt tggaaagtta tgaataagtt tcggattcta ttttcaaaag gaaccttact
    17281 aaatcgtctg ttgcggaaat acgcggttat catgatcggt gtaacgatga ctgctaccgt
    17341 tatttttagt atccatactt gggaacaaaa tcaaaaacag gcagaaaaca tgacaagtga
    17401 tgccgttcaa agcacaagca gaatgctgaa tgataaaaca accttgagta ggatcatcaa
    17461 aaaccagctt gtagggaatt ccgaaaagat cgaaaatgtg acgacctact taacaaaacc
    17521 catagatcag tatctgatgt atgtatacga acagcaaaac agtaccgatg aattagtttc
    17581 tttcccaaat caaatcaaag atctctacgc aaattatgaa gaactaagtg cgatatatat
    17641 cgtgttgaat caactaccgg agtattatga atcttctaga gaaaacaaag gtggggaaat
    17701 aaaaaagggg acaccaaaat tactagacgc cttttatatc aagttgccta tcacacaagg
    17761 aggagctgag tcaggaagta tttttattgg ttttcaaaag aaagaactag atatgatttt
    17821 agaaaatcta acgtcattca atggactctc tttatacatg atttctggaa cgacaaatcg
    17881 actatacaca ttccatgata ataaaatagc taaagaaacg tttgaaagac aagaaatgat
    17941 catcacagag agtctgaaga aaaattcaca attgccgatt gacgatctgg aaaaaaacaa
    18001 ttttgttcaa caccaagaac tgtctgatga ttatcgtata ttagctgtac tagataaaaa
    18061 aagtgtaaaa atcgaaacgg gaaaaaatct tgccccgctg atcattggta tcattatttt
    18121 agacaatata ctgcttatct ttctttataa aacattcaaa cgatattcac agcaagtatc
    18181 tatcgtcatg caggccatgg atcaaacagc agcagggaat ttagagacgc ggatcgacat
    18241 aacaaacacg gaatatgaac tgaaggaact ttcaatcggt atcaacgaaa tgttggatag
    18301 catcaatcag tttgtagaag acatctacaa attagaaatc aaacagcaag acgcacatat
    18361 gcgggcgctt caagcacaga tcaatccaca ctttttatat aatacattag aatatatccg
    18421 aatgtatgca atcagcgaag gaagtgaaga attagcagat gttgtgtatg cgttttccgc
    18481 tttgcttcga aacaatacga atcaagagaa aacgatcact ttaaaagaag aactggactt
    18541 ttgcgaaaaa tatgtctatt tgtatcaaat gcgttatcct aatcgagtag cctatcattt
    18601 tatgatcgat cctgatttgg aaaagatcga agtacctaaa tttgtgatcc agccattagt
    18661 tgaaaattat tttaaacacg gtattgattt tactcgattc gataatgcgc taagtgtcaa
    18721 agtcttgcag gaaggcaaac gggtaaggat catcatcaaa gataatggaa aagggatgac
    18781 tgaaaaacgg ctaaaacaga ttgaagaaaa actgtctcat cctaaagtgg aacttcacgg
    18841 ttctatcggt ctgcaaaacg tgaatgaacg tttacgtgcc agcttcggca gcagttatta
    18901 tatgtcgctt gagaataatg agacaggtgg actgaccgtt tctattacat tcaaggaggg
    18961 atgagcgtgt atcaagtgtt attggtagat gatgaataca tgattttgaa tgggttaaaa
    19021 aagataattg attggcaaag tttaggcttt caaatagtag caacagcaga gaatgccaaa
    19081 gaaggactag ctgtacttga acagcgacag attgatttag tagtaacgga tgtgacaatg
    19141 ccagaaataa atggactgga atttattgaa gcggctcaaa aagaacgtca taattttgaa
    19201 tttatgattt tatcaggata ccaagaattc gattatttga aaggaggtat gcaattaggc
    19261 gccgtgaatt atttgatgaa acccgtgaat aagttcgaat tggttgaaag cctcaagaaa
    19321 atcaaaacac ggttagatca gcaaaatgaa caaaaaaatc aacaagaaat ctatcaagaa
    19381 ctgttattca gtcaatggtt gaatgaagaa ttggatgaaa taagtgaaga agaaataata
    19441 ggacaaataa gtgagaagca gcataggata gtattgattc aactggcaag aactcatgat
    19501 actcttatca atagatggtt gcaagaacgg caacaaatct tttactatca acgaaattac
    19561 ggagaccgtg tattgatcac gttgttgttg gaggcagatg aagttgatgg cttctgttgt
    19621 tttgttcaag aaaactttcc tgatcaggaa tggctgatca gtatcgggga agagacagat
    19681 tctttggaca aaatccctga aacattccaa caagcgaaag acaatctaca gttgcatcaa
    19741 ttttatggag ataaagacga gcatgttttt tacgcagtac aagcagaaac aaaagatcaa
    19801 accatcgatt tttcaagttt cagacgcgtg ctgcaaaata aacaattaga agtcgctcaa
    19861 aaaatgatca ctgatttttt tgaacagttt cagttagcag ccatgatgcc ggaagatata
    19921 cgatattcca gttttttact gtttatggag attcaacggg aattgattga tctggaagat
    19981 gaggaatatt tacaaggaat cgaaaaaatc catcaggcaa aaacggtgca agaacttcag
    20041 cagctacttc tgtcattcat caaaaagcat cagagacaga aaaagtacag tgacaatgtg
    20101 gaaaaagtaa tcgtaatcct gcaccagcat tatcaagaac cacttacact caaagaagtg
    20161 tcagaaaacc tgcatttaaa tgtcatgtat ctaggacagc tgttcaaaaa agaaacgaaa
    20221 aaaagttttt cagcttattt gaaccattta cgtatggaaa aagcgaaaca actgcttttg
    20281 cacagtaatc aaaacatcaa tgagatagca agtgagattg gctacaacaa caccacgtat
    20341 ttctctaaat tattcaagaa aatcgtcggc agatccccta aagagtaccg agaaaacagt
    20401 ggagaatcat aaaagaaaaa gccaatgtaa tgaaataact tataaaaaat tgtctagaaa
    20461 tctaattttg ggctgttctt attacttgag gcaaggcagc tttttcatga cctctcgata
    20521 aacggagttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtggaaag
    20581 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac gacctctcca
    20641 ctaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaatggca aaatatgagg
    20701 aactattttg ccatttgtct ctctaatact caggtcagaa cgctgatttc acaacctctc
    20761 aataaacggt gttcaaaagc ttgcatctgc ggtaataaga ccaaccaaac gaaaaatatg
    20821 aggatctatt tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
    20881 cttgttaatt atagtttgat aaggaaaaag gcaaatcttt tgcttgatat ttcttagggt
    20941 gagggatgat ccctaattgg taagagatcg tgccgccttt cagcaaatct tgatgggtaa
    21001 aatataattt cgtatattct ttgtccatcc gtttgatttt atggataaat tgttgctgcg
    21061 gccgattagg cgttgcttca atcgtcaatt cctttccatt tgacaaaagc aaaacagcct
    21121 tgtcaattaa tggcataccg ataacatatt caccggttcc aggcgtgaca ggataaaatc
    21181 ccagactatt gaagatatac caagcggcca tacttccatt gtcctcatct ccagggtaac
    21241 ccattggaga atcgtcgaag gtctgagtca tcaactgttt aagcagtggc tgtgccattt
    21301 caggttgtcc tatataactg aacaagtaag gatagtgaaa acttggctgg tttgaaatag
    21361 ctaattgtcc aaattctatc gcggccatct cactcatttc gtggatctca aaaccatatc
    21421 ctcctgggtc aaacgttggg ggctgattgc acagctcaat gagtttttct ttatatctct
    21481 cactgccacc atattttccg atcagtcctg aaaaatcgtg ataaacagca aagctgcttt
    21541 gccaagcact tccttctgca taatcttgtc cccaacgatt agctgaaaat ggttctctga
    21601 acttgccatc tttatctttt gaacgcataa atcctgactc atagtcaaag atattttgat
    21661 agttcaatga ttgctgctga tatttcttcg tcatttcctc ttcttctagt accttggcaa
    21721 cttggctaat acaaaaatcg ctataagcat agtctaatgt gtgattgact gattcgtggt
    21781 agtgattagg aacatagcca tatgtcaagt aatcttctgt tccttggcgt ccataatttg
    21841 gatgtttgct ttgaaccgta gcaccttttt ccattgccgt tagaaattca ggcatcaagt
    21901 ctcgtcggat atcttttaca gcagcatctg caatgaccgc atcgatcaat gtcccaggca
    21961 taagtccacg ttcatcagga gacagccatt ttggcaaata gccactttct ctatagctgt
    22021 tcaaaaaacc ttccagcatt tcttcgtatt tttctacagc aatcaaagaa taaagaggat
    22081 aaaccgtttt atacgtatcc caaaacccat tatttgtata gagaacacca ggttttatag
    22141 atcgactagt cgtatcatag tggatttttt gcccactagg atcaatttca taaaatgttt
    22201 gtggaaataa aaacacccga tataaattat gataaaacgt ccgcaattgt tgttgatcat
    22261 ggtgtgtgag ctgtatacga tcaaaatagg actgccattg ttcttgactg tgagcaaggt
    22321 aagtctcttt gctttggttt tgttctagtt ccaggttata agctgcttgt tccgagctga
    22381 taaaagatgt cccaaatcgg atagtttgtt tttttgtctg ttcaaaatac aacgggatcg
    22441 agtcgctatt tttgttggaa acagctaact catcaattgt taaaggatat tcaaatctca
    22501 atataaaata aaaagaaaaa tcaggatctt ctgaaccagc aaaatgtgtg atttttcctt
    22561 ccactgtgta aggatctata acaaagagct gatggcgtcc cggaaaggat aaaagtaaac
    22621 cgctgtcatt tttgctgtaa tcaatagata agattccgcc atacatgcta ggtatcaatg
    22681 tagaacagat gccataacgt aattgacaga tcgacagatg agtcgggcaa aatatacttt
    22741 cttctgggcg gtaagaactt tgagcatgga aaagggtgtt ctcctgtagt tttccattga
    22801 tcggcgtaaa cagcatatga ctgaaatcac ccatccaagg acttggttga tgggtaagac
    22861 ggtagccttg aaaaacgcgg tcatcaggat gaaaccacca gctgccgttt tgatccgtag
    22921 tttgcggtgc aaaataattc atcccaaaag gaacgccagt ataaggctgg cagttcccat
    22981 tagagaaatt tggctgattc gctgttgcat gtctagtatc tatttgaccg atattcattt
    23041 tatttctcct ttgttgattg tcttttgatg aatgaaactg gaaatttact atcggatggt
    23101 cgatcttttg tctctaacca agtgaccaaa gcatctcccg caagcttacc taactgttca
    23161 aaatcttgtg cgatggttgt cagtggcggg tcaacaagag ctgctgcttg gatatcatca
    23221 aatccgataa ttgaaaaatc ttcagggata tgataaccat gctgtctcaa gagattcatg
    23281 gtctggatag cgacaacatc gttttcacaa acaaaagcag ttacttcata cttagtgata
    23341 taagaaagca attcatctaa tgtcgcgctt ttgtcatcca gtgaagtgtg gaaagtcaaa
    23401 ccagctttgt ccaatgcttc aaggtaacca aggtaacgct gtcgaacgga ttgtggatga
    23461 gtctgcgtac caaacaaata agcgattttt gaatgtccct gttcgatcaa ataagaggca
    23521 gcaagcgcac cgccttggaa attatcggaa agaacggtag gaaaaggtaa ctcatagatt
    23581 tttttatcta atatgacgac agggaattct ttcaatgaaa gctcaaaaag taagtcaaga
    23641 tgttgttctg tatcaagagc ataataaatc aatccgtcaa attcattgat tagctctttt
    23701 gctgttttat tttgtagaaa gtcaagcgtc gtcatcatga catcaatctg tttatcttgc
    23761 atcacagggt tcaatccctt gctaaaatca ccaacagaca aatcagcgac atatggaaga
    23821 agaaacagaa tccgtcgtgt ttttgtagga gaagcaagaa tctgttccgg agaagcggat
    23881 ttaacgaaac tgcctttccc gcgaacgcga taaataagtc ctaattgctc taattcagtc
    23941 aatgcacgtt tggaagtgat tctgcttact ttataggtac gtgaaagttc tttttcagtt
    24001 ggtagttggc tgtctacagg taattcacca gttaaaatag ccgtttgcaa atcattaaat
    24061 attgtctggt acaatggttg attcatataa acacctctta atgatatatc atattcaaat
    24121 aataccataa tggatgaaaa agtaaataaa aagatatttt ttgaaagcgt agacataatt
    24181 ggctgtatga agtataatct ttaaagatat atcaaataga aagaaggttt tattagtggc
    24241 ttataaagat ataccaaaat cggtgcagaa atttatggaa gatatcacac aaaaatgtgg
    24301 ggatatccat gcggactggg caaagaattt tacagcagca tttgccaata ctctgttgac
    24361 aacagtcaaa aagcaaaatg acggaaccac ctttttattg acgggagaca ttccagcgat
    24421 gtggttaaga gactctactg cacaagttcg tccttattta gtgattgcaa aagaagatga
    24481 ggaattagct gacatgatca gcgggttagt caaaaagcaa tttttttata tcaatatcga
    24541 tccatatgcg aatgccttca acgaagaagc taacggtgct ggccaccaaa cagatcatac
    24601 aaaaatgaac gattggatct gggaaagaaa atatgaaatt gattctttat gctacccaat
    24661 acagttggcc tatcttcttt acaaaaatac aggaaaaact gatcaattca atcaatcttt
    24721 tattgaaggt atcaaaaaaa tcttggacgt gttcaaaaca gagcaagatc atgaacactc
    24781 tccttataca tttgtacgag acacgacaag aaaagaagat acgctgatca atgacgggcg
    24841 aggtgcaaaa acagctttta caggaatgac atggtctggt ttccgtccta gcgatgatac
    24901 ttgccgttat ggatatctgg taccatccaa tatgtttgcg gtggtcgtgt taggttatgt
    24961 ggaagaaatt tttcaaacga tactcgatga taaagaaatt gtacagcaaa cacagaaatt
    25021 gaagaaagag atccaagagg ggattcagaa tcacggacga acggttaatc aaaaagggca
    25081 aacgatttat gcatatgaag tagatggaat gggaaacgcc acagtcatgg atgacagtaa
    25141 tgtgcccaat ctgatcgccg caccttactt gggatattgt tctgaacaag aggaagatta
    25201 tctgcaaaca agaagaacat tgttgagtaa agaaaatcca tatttctatg aaggaaagta
    25261 cgcaaaagga attggatcct cccatacgcc agaaaaatac gtttggccga ttgcattagc
    25321 tatggaagga atgacaacgt cagacaaatc tgaaaaagag cggatattga atttacttgt
    25381 agcaactgac gcaggaacac accttatgca cgaaggattc gatgtgaaca atcctgaaaa
    25441 ttatacgaga gaatggtttt cttgggcaaa tatgatgttt tgcgaattag tcatggatta
    25501 ctttgatatt agagtagaaa agtaggaggt atcgtaaatg aaaaagaaag tatatatcat
    25561 ttctcatagc cactgggatc gtgaatggta catggcttac gaacagcatc acatgcgact
    25621 gattgaattg atagacgatc tactagagct atttgaagtt gatccgtcct tcaacagttt
    25681 tcatttggat gggcaaacga ttattttaga tgactatctt caagtaaggc cagaaaaaag
    25741 acaggcaatc caacaagcaa tcaatgaagg aaaattgaga attggaccat tttatatctt
    25801 gcaagatgat tttcttatca gctcggaatc gaacgtgaga aatatgctga ttggcatgga
    25861 agaaagccgt aaatggggaa cccctgtcat gttaggctat tttcccgata catttggaaa
    25921 tatgggacag acgcctcaat taatgaaaca agctggtatt tcagcagcag cgtttggccg
    25981 tggtgtaaaa ccaattggct ttgataatca agttttggaa gcagaaaact attcttccca
    26041 atactcagag atgtggtgga aaggacctga tcaaacagca atctttggtc tgctatttgc
    26101 caattggtat agcaacggga atgagattcc agtagaaaaa gaagcagctc ttgccttttg
    26161 gaagcaaaaa ttagcggatg cagaacaata tgcctctacg aaccacttat tgatgatgaa
    26221 tggtgttgat catcaaccgg tacaaaaaga tatatcaaaa gcaatccatc tcgcaaatga
    26281 actgtttcca gattacgagt ttattcattc taattttact gactatctgg aagcagttca
    26341 aaaagatgtg ccagaagact taggctcagt agaaggagaa ttgacaagtc aagaaacgga
    26401 tggctggtat actttagcca atacagcatc tgctcgggtt tacttaaaac aatggaatac
    26461 caaggtccag cgacagctag aaaatattac agaaccactt gcgacgatgg cttatgaagt
    26521 tagtggaaac tatccccatg atcaattaga ttatgcttgg aagacattaa tgcaaaatca
    26581 tcctcacgac agtatttgcg gttgttcagt cgattctgtc catcgggaaa tgatacctcg
    26641 ttttgaaaaa gcagatgaag ttggaaagta tcttgcacaa gattccttag aacagctgac
    26701 cgcagcaatc gatacgaccg gattcccaaa agacagcttt ccatttgtca ttgtaaatac
    26761 tgcaggaatg gataaaactg gtgaagcaga aataaccatc gaattagaaa gaaaaagatt
    26821 cgctgaagga atacctgaac aactatatca agagttagaa aatttgccta aacgtaagta
    26881 tcatgtagaa acaaaaagtg gggccactat accagctatt ttatctgaag aaactgtcca
    26941 atttggttat gatttgccga aagaccgttt ccgtgtgcca tatatggcaa gaatgatcaa
    27001 agtgacttta ccattagaaa acatgccagc attttcttgg gaaacattcg cgcttgtaga
    27061 aggggaagcg aaagcagaag aaaaggaaac aatgattcat cagagtggac gaatcatcga
    27121 aaacggccct ctacatttga cgattgaaaa aaatggtacc attacaatgg aagatcgcaa
    27181 aaacaaacga aagttgaata atctgcatat ttttgaagat atcggcgata taggaaatga
    27241 gtatatcttt aaacaacctt tctgtgatca gccaatccta tccagtaaca aagaaaactc
    27301 tgaagtaaaa gtattagtag atacaccgga aatagcaaaa ataagtattc tacaggaaat
    27361 ggaaatccct gtttctgcag acgagcgttt agaaaaagaa cagcagatgg ttgtggaatt
    27421 tcgttatcga aaagcagaac gatccaaaga aaaacgaatc cttcaaataa aaacaatcat
    27481 gacaatcaga aaagatagta aaaaaatcga ctttgaaacg actctcgaca atcagatgaa
    27541 agatcatcgc ttacgtgtct tgtttcctac aaagttacat gtggaacatc acgaagcaga
    27601 tagtattttt gaagtggtga agcgcccaaa ccatgtgtct aaaagttggg aaaaccccac
    27661 gaatccgcaa catcagcaag catttgtcaa tattcatgat gaagagtacg gtgtaacagt
    27721 cggaaacttt ggtttgaatg aatatgaagt aacagaagac ggacaaatcg cagtgacgct
    27781 acttcgcagt gtgggagaac taggcgattg gggatatttt ccaacaccag aagcacagtg
    27841 tctaggtgag catcggttca attacagcat tgaactacat ggaccagaag aaaaattctc
    27901 tacttatttg catgcatacg cagcacagat tcctttttcc actcaacaaa tcaaacatca
    27961 tgaaggaaca ttgatttcaa aacaacaata tttaacgata aaaagtgaaa cgtttgcaat
    28021 tacagctttg aaacgcagca agtttagtga taaagtagta gtaagaggtt tcaatatgtc
    28081 tagtcattta gaaaaattgg aaataactaa agacaatgga aaaactgtca tattgaatct
    28141 gcttgaagaa ccaacgaagc aagcggttgt cccaataatc caaccttatg agattcggac
    28201 aatcggattc gaggaggaaa acgaatgtat ggcggaatag aagcaggcgg aacaaaattt
    28261 gtgtgtgcgg tttcagatgg tggagaaatt ttagaa
//
LOCUS       DASJTM010000032        27500 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJTM010000032 DASJTM010000000
VERSION     DASJTM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.032"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1047
                     /locus_tag="U4B29_001277"
     CDS             <1..1047
                     /locus_tag="U4B29_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2106237.1"
                     /translation="FSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKA
                     LDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEIL
                     SKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRD
                     VSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQ
                     DFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVK
                     LRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPY
                     NFILKAVTGLNIET"
     gene            1159..2610
                     /locus_tag="U4B29_001278"
     CDS             1159..2610
                     /locus_tag="U4B29_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN2106238.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2959..3591
                     /locus_tag="U4B29_001279"
     CDS             2959..3591
                     /locus_tag="U4B29_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN2106239.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4179..5618
                     /locus_tag="U4B29_001280"
     CDS             4179..5618
                     /locus_tag="U4B29_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2106240.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5913..6131
                     /locus_tag="U4B29_001281"
                     /pseudo
     CDS             5913..6131
                     /locus_tag="U4B29_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6380..6595
                     /locus_tag="U4B29_001282"
     CDS             6380..6595
                     /locus_tag="U4B29_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106241.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6812..7068)
                     /locus_tag="U4B29_001283"
                     /pseudo
     CDS             complement(6812..7068)
                     /locus_tag="U4B29_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7255..8166)
                     /locus_tag="U4B29_001284"
     CDS             complement(7255..8166)
                     /locus_tag="U4B29_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2106242.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8185..9189)
                     /locus_tag="U4B29_001285"
     CDS             complement(8185..9189)
                     /locus_tag="U4B29_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN2106243.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9186..11309)
                     /locus_tag="U4B29_001286"
     CDS             complement(9186..11309)
                     /locus_tag="U4B29_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2106244.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11314..13761)
                     /locus_tag="U4B29_001287"
     CDS             complement(11314..13761)
                     /locus_tag="U4B29_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2106245.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13748..14137)
                     /locus_tag="U4B29_001288"
     CDS             complement(13748..14137)
                     /locus_tag="U4B29_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2106246.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14197..14700)
                     /locus_tag="U4B29_001289"
     CDS             complement(14197..14700)
                     /locus_tag="U4B29_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2106247.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14713..14937)
                     /locus_tag="U4B29_001290"
     CDS             complement(14713..14937)
                     /locus_tag="U4B29_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106248.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(15041..16951)
                     /gene="ltrA"
                     /locus_tag="U4B29_001291"
     CDS             complement(15041..16951)
                     /gene="ltrA"
                     /locus_tag="U4B29_001291"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2106249.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(17697..17834)
                     /locus_tag="U4B29_001292"
     CDS             complement(17697..17834)
                     /locus_tag="U4B29_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2106250.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17831..19015)
                     /locus_tag="U4B29_001293"
     CDS             complement(17831..19015)
                     /locus_tag="U4B29_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2106251.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(19274..19564)
                     /locus_tag="U4B29_001294"
     CDS             complement(19274..19564)
                     /locus_tag="U4B29_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106252.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(19554..20696)
                     /gene="dcm"
                     /locus_tag="U4B29_001295"
                     /pseudo
     CDS             complement(19554..20696)
                     /gene="dcm"
                     /locus_tag="U4B29_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(20772..20936)
                     /locus_tag="U4B29_001296"
     CDS             complement(20772..20936)
                     /locus_tag="U4B29_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106253.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20929..21603)
                     /locus_tag="U4B29_001297"
     CDS             complement(20929..21603)
                     /locus_tag="U4B29_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2106254.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(21681..23021)
                     /locus_tag="U4B29_001298"
     CDS             complement(21681..23021)
                     /locus_tag="U4B29_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2106255.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(23104..23439)
                     /locus_tag="U4B29_001299"
     CDS             complement(23104..23439)
                     /locus_tag="U4B29_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN2106256.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(23449..23823)
                     /locus_tag="U4B29_001300"
     CDS             complement(23449..23823)
                     /locus_tag="U4B29_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2106257.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23836..24150)
                     /locus_tag="U4B29_001301"
     CDS             complement(23836..24150)
                     /locus_tag="U4B29_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2106258.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(24263..27490)
                     /gene="fss3"
                     /locus_tag="U4B29_001302"
     CDS             complement(24263..27490)
                     /gene="fss3"
                     /locus_tag="U4B29_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2106259.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         8054 a         5652 c         4794 g         9000 t
ORIGIN      
        1 ttctctcaag aatatgacaa agttatatta gctacaggtt caaagcaaat tatgctagat
       61 ataccgggga ataatttagc taatattttt tcgataaaga attttccaaa tgcaaaagag
      121 atttttaaag ctttggatga tagtactatt aaaaaagtag gaattattgg agcaggatat
      181 attggagttg aaattgctga agccataaga aaaagaggga aagaagtata tcttttcgat
      241 gttgcagata gagtgttgtc gacttattac gaccgtagtt tttcagataa agttgaagaa
      301 atactatcaa aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa atatttagga
      361 agaaaaaaga ttgaaaaaat agtcactaca agtggtaccc aatactctat ggatatggtc
      421 gtacaagcta taggatttct atctaatagt ccaattgggg aaaaggaact cttgagagat
      481 gttagtggag catatctaac taatgaatac cagcaaacaa atataaaaga tatatatgct
      541 attggggatt gtgctacaac tttttttacg tccattaata gaagtagtgt tgatttttct
      601 atttctaatg ctctaagaac agcttatatt gcaactcaac atattaacaa tcaagatttt
      661 acaccttctg gttcccaatt tacaaatggc ttttctatat ttaattacaa tttttacgca
      721 gcaggtttga atttaaaaac agcacgatta ttaagtgagt ctatagatta tatagaaata
      781 gaagatttca ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc
      841 tttgaaaaaa atacaaagag aatagtaggt gtacaacttt gtagtcaaga agatttatct
      901 ggactattat caatgttttc tcttgctata gaagaacaaa ttacattaga caaattgaaa
      961 atgttagatt atttatttca tcctagtttt agccaaccct ataattttat tttaaaggca
     1021 gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa atatactgta
     1081 ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta ttaaagagaa
     1141 aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt tatattaatg
     1201 tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc aacatacaaa
     1261 attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac agcagagagt
     1321 agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc cgagtcttca
     1381 acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcatca
     1441 agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt
     1501 acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat aacttcttcg
     1561 aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa aacggaagga
     1621 caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt ccaatcacaa
     1681 ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt agaaaatttt
     1741 attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt atatgcttct
     1801 gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt agctcaggca
     1861 ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt cattatggta
     1921 acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt tagagtgtat
     1981 gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg aattagtgga
     2041 aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg agaagcaact
     2101 aaattcctta caggacggta tgcaacagat acacaatact atttgaagct aaatgaatta
     2161 attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca aatgagtaag
     2221 actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg aaatcgtgga
     2281 aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat tcatgcagtt
     2341 aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta cgtggtgata
     2401 caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct agtaaaaact
     2461 ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg gaatagtact
     2521 ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag catgttagtt
     2581 gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca aagtaacaag
     2641 aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg atatctcgat
     2701 tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta ttattgccgt
     2761 ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct atcttgagga
     2821 tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca gactaagaaa
     2881 aattggaaat atttttttct gatttattaa aagttaattg aaattatatg gtacttcttt
     2941 cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt tgaggagatt
     3001 actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga aacgattgta
     3061 tatcgatcaa acttactatc aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat
     3121 aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc aatcaagcca
     3181 actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa
     3241 aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa
     3301 tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa gtatcctttg
     3361 gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt
     3421 gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg tttaaaaagg
     3481 caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc aaaattaccg
     3541 aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata gattgcgtat
     3601 ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa gagtgcagct
     3661 caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa atatcgaggt
     3721 aatcattaat tattgtggat aataattaac agtgatatat aaacatctat aatatattat
     3781 tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa tgtatttgaa
     3841 aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta
     3901 gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga agaacaatga
     3961 agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt tactatagag
     4021 taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa atcgaaatta
     4081 ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat aggttatatt
     4141 gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat caagaagatg
     4201 caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt accttcatgt
     4261 ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat gatatgggca
     4321 taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt
     4381 tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt cggattggta
     4441 cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta ggtattttat
     4501 tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca aatgccggaa
     4561 tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca ataggtgcgt
     4621 ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg tcccaatttt
     4681 cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg ttgattgctg
     4741 aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga gggggctttg
     4801 ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg caagaaagtg
     4861 gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc tttgtcagct
     4921 ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt tttaagaaca
     4981 agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac ttctttgtca
     5041 atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc
     5101 tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc ggctacctga
     5161 ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg tcggtaaccc
     5221 aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt gtcgctactg
     5281 ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg gttatgagct
     5341 cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg
     5401 ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg tctgctaaga
     5461 tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta tatggaatga
     5521 agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg actatctggc
     5581 gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt tgaagaattt
     5641 ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg aagtgaactt
     5701 ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg agatgcttga
     5761 tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat cccaccgatg
     5821 gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat ctgcataaga
     5881 atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc attaagatag
     5941 ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat ggtaagaagt
     6001 cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat cgaaacgtca
     6061 acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt agaaaacatc
     6121 aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg tttaggcttg
     6181 caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt aatactatga
     6241 aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag atgaccacta
     6301 cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag tagtctatga
     6361 gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa taaaaaaaac
     6421 ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt taagcacaat
     6481 aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag atgctataaa
     6541 aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga gataattatc
     6601 gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt gaagggggag
     6661 attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga tatctcttat
     6721 taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat aatcgtattc
     6781 aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt aggcattgct
     6841 gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt attctttatc
     6901 acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa aggcagtttt
     6961 tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta ctaaaataag
     7021 ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg tatatcaacc
     7081 atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat gtcttaattg
     7141 tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg gcgacttctg
     7201 cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc attattattt
     7261 aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact gaaagtagct
     7321 agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa aattttttcc
     7381 gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat tgattggtcg
     7441 catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg gataaagttt
     7501 aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc
     7561 gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca ttgttggcaa
     7621 actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat atgcagaaga
     7681 aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg aaacgacaaa
     7741 ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg aactcgtggg
     7801 aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa ggtactgttc
     7861 gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa aatattcttt
     7921 cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct cttttataac
     7981 cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc
     8041 aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct tacccacgga
     8101 tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt caatttttat
     8161 cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa gatgctgccg
     8221 ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa acatgcgatt
     8281 ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg ttgaatgaaa
     8341 gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg catcatattg
     8401 atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt gggtcaagcc
     8461 actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg ttccttcata
     8521 cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag ttgtagttag
     8581 gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc acccaccatt
     8641 ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt tttcctttga
     8701 aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat gatctagata
     8761 cccgccacca tagttatagg cctgaataat actatcttga tcacacccta gttctttgga
     8821 acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct cggtacttaa
     8881 tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca ttgtgccacc
     8941 agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat ccgattcttt
     9001 ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac tagttccgcc
     9061 aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca aacccacaca
     9121 catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac ccactttctt
     9181 caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct tttttgtact
     9241 ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa agaatttttc
     9301 ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag ttttcgtttg
     9361 atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt cagcatcata
     9421 tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt tccctgacga
     9481 tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt
     9541 tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg cttgaattct
     9601 tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa attcgttggc
     9661 gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc ttgggtatcc
     9721 aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt attatttgaa
     9781 actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc
     9841 tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt tgccaactta
     9901 tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg attgagttgc
     9961 cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc cattgactgg
    10021 ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa atagataccg
    10081 gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc cgaaatattg
    10141 aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc
    10201 ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata agtcggcaac
    10261 atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag aatttgcgag
    10321 aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc
    10381 aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt cgcattgtca
    10441 ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt ttcatctgga
    10501 ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt ggaatcatca
    10561 aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa
    10621 tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt tccaagggta
    10681 agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata ggtaggcgca
    10741 taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac ggctcgaact
    10801 gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc cagagctaaa
    10861 atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt ttcagtaata
    10921 ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat gaaatctaat
    10981 ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact caccgtccaa
    11041 atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc atcaccccaa
    11101 ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata gttgttcagt
    11161 gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc tgtcgcctcg
    11221 gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat aaaaacgatt
    11281 cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt gaacaggtgg
    11341 acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa ccacgcctac
    11401 ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta ggcgtttttg
    11461 attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga tttcatgaat
    11521 atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt catccagtaa
    11581 atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc cagcacgaac
    11641 gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg cctcatctaa
    11701 gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa aagtggaaat
    11761 gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa aagtggtttc
    11821 tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat ctaaactaat
    11881 cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat cggtaaagga
    11941 ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa gctcattaat
    12001 tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct tacgcaacac
    12061 tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa tatcaattgc
    12121 tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta atccttgatt
    12181 ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg gcaaggtttc
    12241 tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac caaaaaggac
    12301 ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg aaccgaggaa
    12361 tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca atgccggttt
    12421 caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt ccaactcccc
    12481 taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa catactggat
    12541 ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc ccatcatatc
    12601 gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca cttcatcaca
    12661 ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc tcagtttgta
    12721 catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat ccaatgcatc
    12781 taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca agtcttttaa
    12841 ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa tctccacatt
    12901 catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa gttctgagga
    12961 gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat atgattcatg
    13021 attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac gactgggacg
    13081 taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg gtagctgaaa
    13141 ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca aatacatcag
    13201 atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt ccattagttt
    13261 ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat cgcccatcaa
    13321 atgatgattc actccataga caaattcttg aaagccggag aagaaagatt tcttgagatt
    13381 ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc
    13441 aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt
    13501 cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg tggcacgaat
    13561 actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa
    13621 ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg agtaattgta
    13681 aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa gattattttc
    13741 aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt tcgtcccatc
    13801 gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc
    13861 taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt tgcgacatga
    13921 accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct atcatagata
    13981 gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac caagccatct
    14041 ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg accttttcaa
    14101 ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt taaaaagaag
    14161 gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact caaaaacacc
    14221 tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg catcaaaatc
    14281 aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta caatcaagaa
    14341 atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat ccacatgctc
    14401 taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac
    14461 tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct caattggcgt
    14521 atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct cgtattcgtc
    14581 atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa accacgcccc
    14641 caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca tctgttccat
    14701 cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga catccttcac
    14761 cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa agccaatcag
    14821 tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg ccagtaagac
    14881 taaggattgg gcattggtta aaaaccattg atatatggat aggtaagttc ctgatattat
    14941 aaatatctca aacttttccc cccattcaca cggagcgtgc gactttcacc gcactccgcg
    15001 ttccatcgtg tttatattga aaagtattta tgtagtagac ctatatcaag tttaattctt
    15061 gattgccagc taatactcta tatttattta gtcttatgag ctggacttta tttagcttca
    15121 atagagctat atatttttgt attgtttctt ctgttttagc gtgtattaga cggtgtaccg
    15181 cctcttttac caaaataaga tttttataat cgtcatttcc accagctcca gttggtacaa
    15241 tatgatgaca atgtatttca ttagcttgtt gaaattcttc cagagttata gcacattttc
    15301 catgttgagc agaaaatagc gaaattcgat tgtctgcgta ttctattgtg cgattttcca
    15361 aagataattt cattaactgg tacatcagta atttatcaac acttagattg gtgtggataa
    15421 attttcttcc ctctagagtg tagcaattcg ctaaatacac ctttgccatt ggttttctgt
    15481 gttggatata tccaacaggg aaaataggtt cgccctcgcc agcaacatac cgaagcattt
    15541 tagatttacc atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt tttctcaatc
    15601 tgcccctttt gtttgtacct aatctgttag tgaagatagc cattacagct ctattcagtt
    15661 tcatacaatc cagatttaca ttcgttgcaa tctgataata attttgcata cctaatacca
    15721 ttgaattata aagtcttgtt tcttctaatt ccgttttccc ttttgaggga gaagcaattt
    15781 tctttgcctg tgataccagc ttgtcttttt ggtttttgaa ttgtttatca cttatatgtg
    15841 atttaacaac taacttcttt cttctcggta tcattttaat cttgaaacca agaaagtcgg
    15901 agtatctttt tctaacattt acaattcttg ttttttcttg tgatacttcc aattttaatc
    15961 gttgttcaat ccattgagtg attgctattt tggctttttc agcactttcc ttgtagcggc
    16021 agaaaactct aaaatcatct gcataacgaa ccatatacat ttcctttaac tttgttttct
    16081 tcatttcttt gtaagcgttg cttttacatg ggctaccgct tttattgata tgaaccacat
    16141 agttttttgt aacaggattt tcctgccatt ggctttctac ccaatggtct aactcattaa
    16201 gaacaatgtt tgctagtagt ggggatatta ttcccccttg cggagttcct ttctctggat
    16261 atgttattgt accgttagtc attttgattg gagcttttaa tatccttttt aaaatgaata
    16321 tcaagtgctt gtcccgtatt cccatagccc atatctgttt tatcagcttg gaatggttca
    16381 cgttgtcaaa gaaaccctta atatcaaatt caatcacata atgtagctgg ctgatttgta
    16441 ggcggttata ggttgctttt attgcatttt caactgacct attaggtcta aaaccatagc
    16501 tgttttcact aaatttagct tcgcagattg gttctaatac ttgttttata cattgttgta
    16561 taagcctgtc ccacatacaa ggaataccta gaggtctggt tttaccattt ggttttggta
    16621 tttcttttcg cctcactggc ttaggtcgat acccatgtgg actgcctgca acaatgtatc
    16681 ttactttttt gacaactgtt tctgctggca atttgccaat atctttaatt attgtaccgt
    16741 ctgttcctgc tgttttactg cctgcatttg ctttgatatt tcgatatgcc agtaatatat
    16801 tttctctcga taggataata tccataagat gagtgaaaat atctccattc ttacttttgg
    16861 catataaatc atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt aaatcttcta
    16921 cacacaatgt ttcattttta tttttaggca taaggcttca ctcctttact tgaaattgag
    16981 tgagcttttt tatcttactc gtaatcctta ttgaaacaac ataatacttt tctaatatat
    17041 acacgacttg agggcattcc tccattctca ttacaagaat ttcttaggtt cgccctctgc
    17101 ttttcagcat tagttcaact gcttattgtt cttcgtcagt ttattctctt gatgagaacc
    17161 caataccttt cctcgttccg ataatcttat ctatacatat atatccttag gtcactagct
    17221 ataagcctgc tggcttgata ccgcctgtaa cgttatatgg catttcataa cctatattct
    17281 tactttgcca caccagctac gatttctcgt acctttgcat ttctacaaag ttcaaattta
    17341 gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat agatatttta
    17401 tagacccccg acctgttgga tacgctaccc acctctggat agttttcgat agaagatata
    17461 tgttcatcaa ctattacggt atctttcagc cgtctttacc gagcttcaaa cattctgagt
    17521 ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt acttcatcat
    17581 tccacctgtc agattatcag ttctcctaat aaccaaactt tttcgttctt ttattagtgc
    17641 cttagctttt cactaaggaa cgagtcgcac ataaattttg tccgaaattc atcttgtcac
    17701 tttcctttct tttccattgc tcgatccgct gctgcacttg cattcatcaa gcacatcaca
    17761 gtaacaccaa gcatactgcc accaaatacc aaaaaaatat cttttacaat tcccatgacg
    17821 attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc aaaatctttt
    17881 tatgtcggtc tgataagcgt gcattctgta ccatatcctg gatataggtt gtccgattca
    17941 atcgatccaa ctccttagcc attttccacg taggggcgac ttgtcggcct agccaattca
    18001 aggttcgttc aatggtatac ggttttggct cggaagttaa ttgaatctct ttccgattcc
    18061 gtccaataaa acgcccccat tgttcattca agggccagtt ggttcgcctt ttaccttcta
    18121 ctttatccgc aaatcgcaag taacgattga taatggaaaa cgtggtgctc tcaatgctac
    18181 gaccttttaa caaatcttga atcgcatgaa aagcacggtc atttttcagt cgaatctcaa
    18241 aacgattttt cgtctcagtt tggtctaatg gaatccctaa cttgatatat tgttcatagt
    18301 ctttttcata caaacagaaa taaacctcac tcttgagact gccgatatat aaggtattgc
    18361 ccatgccgtc cttttcatca gcctttaaaa gttccccgga acgataactt ttaaaggtac
    18421 gaaacaagga gatacattct tccttctgac attttttcgt tagctcaggt atatccaata
    18481 gccccactcg atcattgatt gccaagtcca agcgtttcat cacgccaccg gcctttagac
    18541 aatcctcgaa aaagtcgtac cagctacgct tttgagcgag taaaaaagtt tcaaattgtc
    18601 ggcaacctcg cccctttaat tctaaaagca cacctttatc ttcttcatga gacaacatca
    18661 ccacaatatc acccatgaca tactgttctt gataggagta aaaggcataa tcttcatgga
    18721 gcatatactc aaatttcaaa tggagaattt cttgaataat ttttcgtgca tccgttgtgg
    18781 gaaagcgaat ccttacatag tcaaacagta acgtcaaagg caaatcggga ttaaagcttt
    18841 ccagttggtc aaaaattttc ctcatggttt ttatagatgc ggttacgcca ccattttcta
    18901 tctgatttaa gtacggccga ctaagtccac aagccgtagc caatttattt tgtgaaacgc
    18961 cgtattcgtt gcgtcggtct tttaatagcc gataatctaa attccgttgt gccaagttct
    19021 cacctccaaa ttcaaggtgt taactttttc aaatgagtta acacctatac aaattactta
    19081 ctatctttga tacaacaagc attcttaatg gttgcttact tattttttgt gtttttgtac
    19141 ccccctgtta gatacggggg tgagacatca tggtcggcta acgccgtcca tgaacaggca
    19201 cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg tcagcgccta acagtcagtt
    19261 gtgttggttt aggttactca tctgtaatac catttacttc caactctgct ttcatttcta
    19321 acaagtattg acgaatatct ggttcttttg cttggtccgc aatccgctca aattctgcaa
    19381 ttacttgatt tatttcaatt cgagcatccg tctcctcatt cattaaatat ggatcgccag
    19441 cgactttgac accattcttg cgaatatcaa attttaaccg tcgcaattgt tcttctaatt
    19501 ctgggtctga ctgaatcttt ccaatcaccc aataaatttc tccaatcaac tcatcataaa
    19561 ccatgtacat ccaactcttt taatcttctg gcaatatcaa aaatgaccgg aactgttaca
    19621 gagtttccgg cttgcttata caactgacta tctgaattga cttgagaatc tcgatcaaaa
    19681 gcccaatcgg gaaagccctg aagtcgccaa cactctctag gtgttagctt ccgaatttga
    19741 tactgatccg tgacgatcgc ttgttggtct cctgttagta aggtatgaac actctgtttt
    19801 cccagacgac ctcggcgact tgtggaatta ggaaaagcca gattcacact gtcttctggc
    19861 ttcacctctt gatattctgt ttcatttttt cgaatcatga tttttgtacc accttgtttc
    19921 gttgacaagg tgggggcggt tccagtaact tcataaactc gattaccttg ttcaaacttt
    19981 cccggtaata gtcctgctac agagattcca tcccttgctg gcagtgcgat tttgattcct
    20041 tcatcacctg ttaaggttgg cgctacgcca tctgaaccat agacttgtcc acccattcct
    20101 cgattggaag gattgatatt taccaaattt ctgatgtttt gatcagatgt tgaagacggt
    20161 ccaatgatag gaaatacttg ttcggtacat gcgtctcgag aatgtccgat aaggaatatc
    20221 cgctcccggc tttggggaat gtaggccttt gagttaagca cctaccactc cacgtcatac
    20281 cccaatccat ccagcgttcg gaggatcgtc tcgaacgtag cccctccttc gtgattgagc
    20341 aatcctcgga cgttctcaag gaataaaagc ttaggtcgga gaagagtggt gaaccgagcg
    20401 atttcaaaga ataaagtacc tcgagtatcg gaaaatcttt gccgctttcc cgcaattgaa
    20461 aaagcttggc acggaaatcc gccgcaaagg atgtccactg gtccgaggga ttgaataaac
    20521 tcatctgata cacttgtgat gtcatgcatt tccacctcct tacttgtatc atgaattgct
    20581 ttataactac gtctagcaaa tttatcaatt tcacaaaaac cgatacaatg atggctcgcc
    20641 tgttccatac ctaaacgaaa gccaccaata ccggcgaata aatctaaaaa tctcatacat
    20701 tacctcattc attgcaaaga aaaaagccaa gaacattttg ctcttggctc atacattatt
    20761 aatttgtcct actatttttt atttaatcca actaatttcc gttgtttttc attggcctga
    20821 tcggctacat atttaatcac cgttttgaac tcttttgcag tagcattttc cgataaatcc
    20881 gcaatgctct tattgtattt ctttacaatt ttcgttactt gttctttctc agtcatgtaa
    20941 ttgaacctcc aaatctttcc tactataccc tagtggtaaa aataattcta gattatccgt
    21001 agcataatga acttcggaaa tattattcgt ataatctttt attatgatat gaagcaatac
    21061 atctggatgt ttactcttta cttttgcaat gttctctctt gatttgaaaa aagcgttaat
    21121 aaatgccttt ttcggcacat gacgtccctc tgcaatttca cgttcttgtg taaatttcca
    21181 agcgatttta gggtcttgat aaacatagaa aatcgtcact cgaaaatgtt ttttcaacgc
    21241 ccgcgagata tttttttcag cacttaatat tgcaaaagtt gcatccagaa taaaagaata
    21301 gccatgatct aagacatatc taaatgcctg ctctaccaac caagaagatg ctttttgaaa
    21361 gagactagag ttttttccat tataatcagg aaactgggtg cgaaagtaat ccgcatcaat
    21421 aatcacatgg ttatcataat tctcagctaa tccaatggca acctctgatt ttccagcccc
    21481 aggactccct gccataaaaa ctgcgtctcg tccaacttcg ggggatttcc ctttgataat
    21541 ataagaaata aacttatcct tatttgcttt tgcatactct aaataagaat cattactttc
    21601 catattaatt atcccctttc ttcctactat ataaaagtat tatacgttac ttgaagtata
    21661 aagtcagtat tgaagatcac tcaatcattc atcttttcta atttactaat tgaatctaag
    21721 aaatcatacc cttttggtac aagtggcgta taaaactcac taatcacact gccacctgta
    21781 tccacataac ctcgaccttt gatgtgcttc ataaagaagt ttttttcgac ctccccaaac
    21841 atcatggaat atccgagttc actcatacga cccaaagcca cgcgaaagtt gaattgatcg
    21901 cggattccgt ctcctaaata tttcgcatcc ggcctttggc aagccaaaat cagaaagaaa
    21961 ccagattgac gacccaacat cacgatttgt ttgagcttat tcaaaatcac cgcactttct
    22021 ttcgtcgtta acatctccat ataagccacg tattcatcaa agattaaaaa gttgggtgga
    22081 agtccaagat aggcataatt ctctcctgtt ttgtagttag acatttcttt cattgccttg
    22141 ctacgagcca tcatacgttc atagaaatct tccacacaag cagaaatttc ttccttttgt
    22201 gaataaacat gaggcatcac cgtacctaaa tcagctaaat ctgcattttt gggatcgagg
    22261 ataaacaatt ctgcatctga cttcaatagt gcttcgataa tggtgaggag gaaataggtc
    22321 ttccctccac ccgtgccacc agcgattaac atatggggta aagaatcata agcccatact
    22381 tgatttttca tcaatcgcag agtcccattt tctgccacta cttcgtctat cccaattcga
    22441 ttggcgatca tatcatacag taaagtatac tccacatagg agtctttgag ttccttttct
    22501 actaactcac aatacagtcc gctttctaac ttcttttcca attttaaaag ctgatcttgg
    22561 tatttcccaa gtgaaatttg aacgcgaata gacagtaatc catctttcat tcgataacag
    22621 attttgggga agtaactgat agtctctctg gttcggctac tattcaaatc tttgaaaaag
    22681 ccttcacttt gagtttgttt cacttcatac caatggttct ctagaatcat ccgagctagc
    22741 ttttgtcgat gccacaagct tcgccaatga tcaagaaaaa agtgaatata aagcaatatc
    22801 atagctcctg aaaaagcgag acttccaagc agacatacta aatcttttat agaccaaacg
    22861 atttcaatag tagaaagtga tagcttagaa atatcaaatt gtctaaactc ttgaaaataa
    22921 aagacgctca tccaaccgat gaacagagcg agtagccagc caagaacaaa gtgataaacc
    22981 aagtgctgat ctcctgcgcg tatgcgatgt cctttgtaca ttttgatggt ttccacctcc
    23041 aatcgaaaaa gcccaatagg atttcattag gcaatttcaa aaagttatgc tagtcattta
    23101 ctgctacgag gaggggagtt gtttcgtttc aaccaattga tttaaaaagc cttcaatagc
    23161 atccggcatc tccacaccaa tttcttccaa aatggattct gcctgccgac ataagttttc
    23221 atttacttcg acaactataa agaaagactc ttcttctcct aaccagtcta gtacatcaga
    23281 gtcttcttca ctgtcgctat tttctccaag taaaccttga tgtataatca gaaacatttt
    23341 ttcatcagag gttaatgctt taacgagttg ttcctcagtc agctcgcctt ttccatattg
    23401 atcgagacaa tctaaaatat gttcaacatt aatttccata atcttcacct atttctttct
    23461 ttcattttgg ttcgtaggat ttccttgatt cggctgccgc ttcaagacta aatcatccgc
    23521 cttaatgaac caattaacgg tagttccttc tcgaaaaaca taattggcaa cggtatccac
    23581 gattggattg accaactcta cctctgcatc ataagcaaac tcgcgtaagg gtacattagc
    23641 tgggatacta acttgaatca ttcgcccttg tgcagaggat tttaaattat aggttcgttc
    23701 ttttacctca ttaccaattg ttccatcctc attttgcttc cgtacttctc ggcgtaatgc
    23761 ggaaaaacgc aacttcccaa atgttaaacc tgaatctacg acaatacctt ctgttaatct
    23821 catcttcttt tcctcctatg ctttcactaa atcatcaaca tacaacacgt aatcagtaaa
    23881 cccacgattc tcaatctcat agcctttggc aaccaaatgg ggattcacgg cttttagcgg
    23941 ttggtcttga tcaaaatcct tttcaccggc ttccgcagct actaccacct caatatcatc
    24001 ggcccgttgc ttactggaat acaaatgata actgcgacca atcaccgtag tattccgtcc
    24061 atatccttct gttataattt ctccttcacc ggcataactt aactttccaa aggtttcagc
    24121 catatctggc acgacaaatt tcaattccat cttcttttcc tcttttcatt gttagttttg
    24181 gatacaaaaa tagcgcgact acttttttag gaggcggtcg cgcacgttgg ttcttaggat
    24241 ttacttgtat catctcatca ccttagttcc ggcgattcca gaaataatag cccgccgtcg
    24301 caaaaatcaa agtaaaacca atgaacagta aaacattgga atttttctcc ccggtttgag
    24361 ggaaggtctt cactgtggga ctagattctt gagagccact tggaacagtt ggtgtttccg
    24421 gttgtttcgg cgtacttagt atggttggaa cttcttttgg tgttaaggtt tgagattttt
    24481 ctttcaaatc ttcattgtgt tttccattga tattcccatc tttatcgtaa ttgatttccg
    24541 caaaagtaaa cttggttcct tctggatatt ttccggtatc tacttttttc gcaagcacag
    24601 ttttggtgaa ttctttatca tttacttcat aatcaatctt gccagatttc caaatttctt
    24661 tgtttgtacc atccggtaat aaagcataca gaattgtttc gaaagcttct tttgagccat
    24721 ccagtacatc atgagtaatc gacacatcat caaacatatc caccacatca ccatgagtaa
    24781 aggtctgaga accgttttct aggtgggcct ttgtttggat agaaacatgg tgttccactg
    24841 tacaactgac agtttgggct tggttattca agctaacatc cttcgctact ggcttatcgc
    24901 ctgcttcaaa agcttcctta ttttcataca catagttgaa taagacaata gttttattta
    24961 aaacttgttc ggcggttaat ttatgctgaa ttttccaagt tcctgttttc tctttgttcg
    25021 ccaacgttgc gcttgtttct gcaatcacca ctggtttggc tttttcatct ttttcttggg
    25081 cagcttttgt cgcatccaca tcaatggctt gcgctaccac ataccagtct tctttgattt
    25141 catgtagatt gtaactcaat ttatcaacta gctcggtaga atccagagaa gttaattctt
    25201 tgtttccgtc tttccaagtc gcaagagaag ttaaactatc ttcttcctca ggcaaatcat
    25261 agagcattaa acggttcaaa ctaacagaaa atgctttgtt cgtcagcttc tcgtaaggaa
    25321 cggtcaccgt tttaattggt tgtttttggt cttgttcggt aatggtaaag acatactcac
    25381 tcttcacaaa gtcttctttg ttttccttaa aagtagaacg gatttctaat ggcgtaatct
    25441 tttgaaaacc ttctggggct tctacttctt caagtaaata atccccataa ggaagatttt
    25501 caaacttgcc ataaccatca aaccctaatt gctcgttata agctgtggtc gcttcatctt
    25561 cggcacctgt gatttcattg gtcccttcca atggcgacac tttaaaggtc agatcattga
    25621 agccagtttc ggtagtgccc gcagctgatc cagcaaattt aaagaaatca aagccaaagc
    25681 gaatcacttg ttcttttgcc gtaacatttc gggtaatcac ggcatttttt tcgctatcat
    25741 ccaccttttt gatggataca ggatactttg tttcatcaag gctatatcct tcgggcgctt
    25801 ttgtttcttg ccaataatac tcgttgatag ctaaatgttt aacggcaact tggttctttt
    25861 cgtctaaagt caaagtgacc gtttcatcag aacctttcgt tcccttcgct aactctggtt
    25921 taaaagcctc actccattta acagcttttc catccttagc agtaaacaag gtgtattcag
    25981 caccctcaaa gactgcctta ccttgtgctt tatccccagt atctttgtct tctttcgtca
    26041 aagtggtttc gccagtgact tcttgatttt gcccttttac gttactggta acaggggcta
    26101 cggtttgatt ggcatacttc aattcgactt ttacaggttt gaacgtattc acgaacccat
    26161 gactagcttt agtttcggtc acataatagg ttcccaattc caacgcatta gcaatctctt
    26221 ttggtgtttc cgcatgaccg ttttcatccg tggtcatttc ttgaacgatt tcaccggttg
    26281 gactgtcttt gcgaatggca aacgtattac cagctaagga ataattgtca ttccaaagat
    26341 cactccctgt ttcaactccg cttttatcca gaatgatttg ccctttttcc cgtgcgtttt
    26401 ttgaagtcac gtaaatggtt tcgcctgctt taatggtggt agtcatgggg gtggtatcaa
    26461 tcgtataagg cgccggtact gatttttcgg taatggttac ttttgtacca tgaggaatct
    26521 catccaatgt agcaatgcct tctttgtcag tagtcacgtc ttttgcaggt aaagtttttc
    26581 caaagtctaa atggaaaacc gttcctggta caacatcacc cgattcttta tcgacttttt
    26641 tgatctttaa agaaccctcc gtttccacat caattttgat tgtgtaggta ttaggtttat
    26701 caatcgcccc ggccattaaa gtctgttggc cagccttttt ataggcgaca ggtgttccgg
    26761 taccagcaga tttcttaagc gtcagcacac cactttcttt tgaattggca tttggggtga
    26821 ttatcaactg attaccattc acacgataat caatgtttgc ggcattttcc acaacctcat
    26881 cgaactctga caaattcaga ttattcgtat ccgtcacagt agtcgattgc cctaatactg
    26941 ttttagccgt actattatgg aaggttggtt tcttctgata atcgtcaatg gcttgattta
    27001 tttttgcctc aatggctgcg atatttacgg cactaccatc tgatcgtttt attgagtgaa
    27061 gggtataccc attaacttct tgccagatca tcttctgtgc cacaatatga gtatctgcat
    27121 ctgtacctac ttttttccat aaaacggaaa ctaatttggc tttatcggac atatctggta
    27181 acgggttttt ctcatagcca ggagtgaact cattgtaaat cgggactcct ggttcaatac
    27241 agaaaatatc ttgtttaaag ccgttgtaca ctgcataatg cggaacagtg acttctgaca
    27301 ttggacgacc atcactaaaa gttccttcaa cagcatattg atgtgctaaa tctagttcta
    27361 cggttaccgt ttgtggatgg gtaatttcat cagcaaaagt aatcgtagga gcaattgctc
    27421 caacaattgc ttgaacagcc aaactcaaaa tagttaatac acataatttc tttttttgtt
    27481 ttttatacat gaaatgttct
//
LOCUS       DASJTM010000033        26804 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJTM010000033 DASJTM010000000
VERSION     DASJTM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.033"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..519
                     /locus_tag="U4B29_001303"
     CDS             <1..519
                     /locus_tag="U4B29_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106260.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            527..1042
                     /locus_tag="U4B29_001304"
     CDS             527..1042
                     /locus_tag="U4B29_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2106261.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="U4B29_001305"
     CDS             1147..1977
                     /locus_tag="U4B29_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN2106262.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="U4B29_001306"
     CDS             1994..2845
                     /locus_tag="U4B29_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2106263.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="U4B29_001307"
     CDS             2842..3201
                     /locus_tag="U4B29_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN2106264.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="U4B29_001308"
     CDS             3216..3377
                     /locus_tag="U4B29_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2106265.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="U4B29_001309"
     CDS             3374..3679
                     /locus_tag="U4B29_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106266.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="U4B29_001310"
     CDS             3679..3996
                     /locus_tag="U4B29_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN2106267.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="U4B29_001311"
     CDS             3993..4388
                     /locus_tag="U4B29_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106268.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="U4B29_001312"
     CDS             4385..4723
                     /locus_tag="U4B29_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106269.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="U4B29_001313"
     CDS             4720..5088
                     /locus_tag="U4B29_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106270.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="U4B29_001314"
     CDS             5085..5390
                     /locus_tag="U4B29_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106271.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="U4B29_001315"
     CDS             5414..5617
                     /locus_tag="U4B29_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2106272.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="U4B29_001316"
     CDS             5614..5910
                     /locus_tag="U4B29_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106273.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="U4B29_001317"
     CDS             5986..6402
                     /locus_tag="U4B29_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2106274.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7117..7284
                     /locus_tag="U4B29_001318"
     CDS             7117..7284
                     /locus_tag="U4B29_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106275.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKY"
     gene            7310..7654
                     /locus_tag="U4B29_001319"
     CDS             7310..7654
                     /locus_tag="U4B29_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2106276.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7659..7940
                     /locus_tag="U4B29_001320"
     CDS             7659..7940
                     /locus_tag="U4B29_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106277.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8043..8357
                     /locus_tag="U4B29_001321"
     CDS             8043..8357
                     /locus_tag="U4B29_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2106278.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8335..10029
                     /locus_tag="U4B29_001322"
     CDS             8335..10029
                     /locus_tag="U4B29_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2106279.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10049..11227
                     /locus_tag="U4B29_001323"
     CDS             10049..11227
                     /locus_tag="U4B29_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2106280.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11190..11876
                     /locus_tag="U4B29_001324"
     CDS             11190..11876
                     /locus_tag="U4B29_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2106281.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11876..13036
                     /locus_tag="U4B29_001325"
     CDS             11876..13036
                     /locus_tag="U4B29_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2106282.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMGKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13046..13921
                     /locus_tag="U4B29_001326"
     CDS             13046..13921
                     /locus_tag="U4B29_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106283.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13918..14229
                     /locus_tag="U4B29_001327"
     CDS             13918..14229
                     /locus_tag="U4B29_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106284.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14219..14572
                     /locus_tag="U4B29_001328"
     CDS             14219..14572
                     /locus_tag="U4B29_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2106285.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14562..14963
                     /locus_tag="U4B29_001329"
     CDS             14562..14963
                     /locus_tag="U4B29_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106286.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14956..15360
                     /locus_tag="U4B29_001330"
     CDS             14956..15360
                     /locus_tag="U4B29_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106287.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15372..15980
                     /locus_tag="U4B29_001331"
     CDS             15372..15980
                     /locus_tag="U4B29_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106288.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16000..16362
                     /locus_tag="U4B29_001332"
     CDS             16000..16362
                     /locus_tag="U4B29_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106289.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16365..16547
                     /locus_tag="U4B29_001333"
     CDS             16365..16547
                     /locus_tag="U4B29_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106290.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16565..19897
                     /locus_tag="U4B29_001334"
     CDS             16565..19897
                     /locus_tag="U4B29_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106291.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            19948..20685
                     /locus_tag="U4B29_001335"
     CDS             19948..20685
                     /locus_tag="U4B29_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106292.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20695..22986
                     /locus_tag="U4B29_001336"
     CDS             20695..22986
                     /locus_tag="U4B29_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106293.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            23010..25091
                     /locus_tag="U4B29_001337"
     CDS             23010..25091
                     /locus_tag="U4B29_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106294.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SAYSFVITYSI"
     gene            25105..25452
                     /locus_tag="U4B29_001338"
     CDS             25105..25452
                     /locus_tag="U4B29_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106295.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            25445..25585
                     /locus_tag="U4B29_001339"
     CDS             25445..25585
                     /locus_tag="U4B29_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2106296.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            25655..26074
                     /locus_tag="U4B29_001340"
     CDS             25655..26074
                     /locus_tag="U4B29_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2106297.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            26071..>26804
                     /locus_tag="U4B29_001341"
     CDS             26071..>26804
                     /locus_tag="U4B29_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2106298.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
BASE COUNT         9622 a         4013 c         5763 g         7406 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgcg taaaggaggc acgacgatgt atagaccaca atacttagaa
     7141 cagaagcatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     7201 agaccaataa agagcgatac atataaacgg aaggaaagca atgaagttat tccattgtat
     7261 ggcaaaagaa tagctaagta ttaaataaga ttgcgaaagg agatgggaca tgaccgagga
     7321 attctataga tggttattac agttgataag agaagatcgt ttggttaagt tctatcagtc
     7381 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
     7441 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
     7501 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
     7561 taatgaagtt catggcagat atcttacagc gttagataaa caagagaaga agatagaaag
     7621 cttcgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gacaatggca
     7681 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
     7741 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
     7801 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
     7861 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
     7921 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
     7981 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
     8041 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
     8101 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
     8161 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
     8221 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
     8281 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
     8341 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
     8401 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
     8461 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
     8521 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
     8581 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
     8641 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
     8701 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
     8761 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
     8821 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
     8881 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
     8941 gattattcaa tgataaatgt ggctacttct ggtggtggta aaaatcgaga ttatagagaa
     9001 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
     9061 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
     9121 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
     9181 gaaaaagctt gtccaactat taattacaat gcaaatttaa aacggaaaat gtttcaagaa
     9241 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
     9301 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
     9361 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
     9421 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
     9481 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
     9541 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
     9601 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
     9661 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
     9721 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
     9781 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
     9841 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
     9901 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
     9961 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    10021 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    10081 cacaaaaaaa gcaacggtta ctttagaaga atacttttgt aaattgcaag ttgattttgt
    10141 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    10201 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    10261 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    10321 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    10381 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    10441 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    10501 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    10561 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    10621 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    10681 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    10741 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    10801 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    10861 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    10921 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    10981 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    11041 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg
    11101 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    11161 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    11221 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    11281 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    11341 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    11401 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    11461 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    11521 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    11581 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    11641 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    11701 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    11761 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    11821 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    11881 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    11941 tggtaacgaa gagtctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    12001 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    12061 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    12121 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    12181 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    12241 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    12301 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    12361 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    12421 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    12481 agatatgcca gtcggaatga tgcgcgacat gggcaaacaa actagcggag aatatgctga
    12541 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    12601 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    12661 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    12721 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    12781 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    12841 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    12901 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    12961 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    13021 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    13081 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    13141 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    13201 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    13261 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    13321 ctatatcgat tcgaatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    13381 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    13441 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    13501 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    13561 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    13621 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    13681 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    13741 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    13801 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    13861 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    13921 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    13981 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    14041 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    14101 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    14161 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    14221 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    14281 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    14341 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    14401 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    14461 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    14521 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    14581 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    14641 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    14701 acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc
    14761 aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag
    14821 atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt
    14881 taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat
    14941 tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa
    15001 cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg
    15061 aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa
    15121 gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac
    15181 attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat
    15241 ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt
    15301 cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag
    15361 ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac ataagtatca
    15421 aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgtaacgaat tgtaatggac
    15481 aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa
    15541 aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag
    15601 tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt
    15661 acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg
    15721 aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct
    15781 ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag
    15841 atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag
    15901 atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta
    15961 cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa
    16021 attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta
    16081 ttgatgatca atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc
    16141 tctatggata ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg
    16201 aacatggtcg attacatttc attcgatgaa tttatagata aatgggattt tgatatggag
    16261 gttgcagtag ctgtatacag tactatgatg ttcaaacaag cacgtgatgc ctatcaaaaa
    16321 gaattcgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag
    16381 ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt
    16441 tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagcgctt
    16501 atgaaggatg gaagaactat gcaataaatc aatcccagaa aaactaaagg aaaggaggta
    16561 aaaaatggct aaaaagagaa cggaagcaga agtaactttc atagctaacg atgacggatt
    16621 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    16681 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    16741 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gtcaacgttt
    16801 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga
    16861 actgattaat caacaaacag ctcagcaaag gttatctaat gatatagata aaacgagtaa
    16921 ggcattggct caatctaaag gtgaatttaa aacgtatgag tcaactatga aagacttaga
    16981 taatgagcaa caacatctaa agtcaagcgc atcattagta gaatccgaat ataaaaaatg
    17041 gcaagcaact gctggccagt ctgctacaga atctgaaaaa ttagcaaaag cacaagaata
    17101 tgtcggcaaa cagagtgata ttgctgaaca aaaaatcgaa gtgctaaaaa aacaattaga
    17161 tgccacacaa aaagaatttg gagaaacatc taccgaagct ttagaaatga aaactaagct
    17221 taatgatgcg gaaagagaat tcgaagaatt aagtaatgct gctaaaaacg ttgatacctc
    17281 cacagtagat gatatcggca aaaaacttga tatgggtaat ttaatggaag ctactgatca
    17341 cttgtcagtg atcggagata agcttattga tgtaggtagt aagtctattg aagcagctgg
    17401 aaaagcacaa gctatgcagg cccaatttaa acaagtcttt ggctctttag aaggggaagc
    17461 acaggacgcc gttgagggaa tggctgaaga atttggaatg ttaccaaata cgatcaagcc
    17521 tgtttttata caatatacgt caatgtttaa aggacttgga tatgatacca aagaagctat
    17581 ggagttagct ggtgatagta ctcagttagc agcagatgca gcagcttttt acgataagtc
    17641 tatggatgat gctagtgaat ctcttaattc atttataaaa gggaactacg aaggtgggga
    17701 gcaaataggt ttatttgcta atgatactca aatggcagct tatgctgtta agcataattt
    17761 gataccagcg actgaaggag caaaaaaagc cagtgaagag tcattgttag ctgttgaaaa
    17821 agcacaatct aagtatgctg atgctattaa gaaacatggt gaaggatctt tagaagcaag
    17881 agaggctgct ttaaaactta aagatgcgca agataaaata aatgaagaat taggcccaca
    17941 aacgcaaaaa tggtctgatt tagatgaggc taccaagcaa gcagttcgag ttcaatatgc
    18001 tgaagatatg caaaaattag ccggtgctac aggtcaggct agtagggagt ctgatggttt
    18061 agaaaaccaa atgacaagag caaagcaagc gttagaagat ttttatgcct cattgggtga
    18121 agatatactg cctgtgttta tcaaagggtt gcaagcagga gcgaaagctt tgcaaggatt
    18181 agccgaatgg tggagtaaac ttgatgggcc aatgaaaaat ttcattttag ctcttggagg
    18241 aattctagca ttattaagca cattagctcc tgttataacc gcagttgtta cgatagttgg
    18301 cacatttggt tctacagttt tgctgccaat aataggaatt atagcgggcg ttgcagctgt
    18361 aatagcgatt gtaattacag cgtttcaaaa ctggggcgca atcactgact ggtttagtga
    18421 tttatggaaa aaatttaccg attggttagg tgatacctgg gaaagtataa aagaaagtgc
    18481 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    18541 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    18601 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    18661 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    18721 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    18781 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    18841 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    18901 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    18961 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    19021 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    19081 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    19141 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    19201 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    19261 gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca ctattagctc
    19321 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    19381 ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag gcatcaaaaa
    19441 gactgttagc gctccaattg attggattag agacaaaatc agtggcattt ttgataattt
    19501 gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca gctttaaccc
    19561 gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag gtatctttac
    19621 taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg aagcagtttt
    19681 acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta gcacatcagt
    19741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    19801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    19861 aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    19921 ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    19981 tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa
    20041 agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca agattactcg
    20101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    20161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    20221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    20281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    20341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    20401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    20461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    20521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    20581 aacaaaatga agaccatgca atatccaacg ttgcaagtgg atattaatca ttttaattgt
    20641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    20701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    20761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    20821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    20881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    20941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    21001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    21061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    21121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    21181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    21241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    21301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    21361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    21421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    21481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    21541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    21601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    21661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    21721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    21781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaatggt
    21841 ggcgaaatca gtgatttaat caatgatatt gtagatcatc aaactgatat gattactggt
    21901 caaaatggtg gttacgtttt attagatcct aaagaagccc ctagtcgtat tttgattatg
    21961 gacacaccag ataagaatac tgcacggaac gttttgcaaa tcaacaacgc tggtattggt
    22021 ttctctaaaa ctggcattaa tggaacgtat gacaccgcat ggacgttaga tggcggattc
    22081 aatgcctcgt ttattacagc tggtgagata gtagggatta ctattagagg tactacatta
    22141 attagtgatg gcactgatta tagaacaagt atagctaatg gcaaaatgac ttggtactca
    22201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    22261 ggcattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    22321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    22381 ccaaatttca gatacagtaa tgctagttat ataaatgatg gtgacggtaa ttctttaggt
    22441 attgatggta gtgcaggtaa ttcatgggaa tttaaagtag ctggtaggac tatgaaattt
    22501 actagtgacg gtatgctaac gttgccaggt tgtttttttg gttcatggga agatgggaaa
    22561 cttgctagat ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    22621 aaaaactcaa ccgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    22681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgatgatgg taaagttcgt
    22741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    22801 acacctacag aactagtttt gtgtgctgtt actcatgaag atgttgacca tttcatcatt
    22861 gaaactagta agccaaacgt attggttaga tggaatttag tggcacatcg actagggtat
    22921 gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    22981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    23041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    23101 tcacggtgac tgttaaaccg tttttaaatg gcttgcagat ggatacgagc ggcggaacat
    23161 ttactttaaa aggaactacg ccatctaacc gttacgtaga tagtgtcgct actagtgtaa
    23221 ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca gggtattata
    23281 aacactgcta tgtagaatat agaaaagacg atcaaatttt aacgacgcaa gatatcattt
    23341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat
    23401 tagaagaatt gattcgaaag tataacgaaa cttttgatgc ttttctggct gaaattaaag
    23461 gtagaatgga tagcttaaat aaacagatta ctgatttaac tggtcaagct aaaacactac
    23521 aagacaagtt agatgctctg aaagaagaaa tttctaaatt aggtaactta catgtgatgt
    23581 actccaacag catcgacttc gggggctatg attatagtgg gaatccgaat ttaatgtcca
    23641 aactaaaatc gagcgatttt aacgttggtt accacgggtc actaactaat gttaacggaa
    23701 tgttacattt tatgtctgat ggtacagggg acattgatat gtttacgcgt attaatacac
    23761 ctcagcttgc tagtggcaaa acatatactc tgagtgcaaa agttcgattt gatgaaggaa
    23821 ctacaggagc tattaataaa ttacgtttgg tatatcgtac atcaccagga gaaaatatat
    23881 tattggaagc aaataataca actattacaa tagatgatgt agggaaagaa ataacaatca
    23941 aaggcacagc taacgttaat tatcaaatca caaatttaga tcgtttttat attagtgtta
    24001 gtttttctaa cagggataaa ataaatggtg gatttaaatt gtacgacatc aaaatcgaag
    24061 aaggctcaac agccactccg tatcagccaa acttactaga tgacccttac tgggtaggta
    24121 aaacatcgtt gggtaagaat attgcaaata aaggtacgac gttcccaatt aaatctagcg
    24181 cgtacgaaat atataaaggt aacacggaag aagagcttat aataggtcaa acgtatacta
    24241 tcacgcttaa aggaacaaaa cccgcaagtc aaacctttgt agcatataat tattggaacg
    24301 ttaattttgg agatctaaaa ccagttgagg gattgacaga cgtatggtct ctaacattca
    24361 caccaacgaa acttgaaccg ggtttgccta aagaccttcg catttttcag tcgcctaaag
    24421 aaacggccgg cgcatgccga attgactggc tcaagatcga aaaaggcgac acacgaaccc
    24481 cgaatattga gcaatataaa taccgaggaa tcggcatgcg agactcaaac aatccaaaag
    24541 attatgtatg ggatctagaa cctagctatg ttgagaataa cctagctaca gatatcaagg
    24601 ttgctgaaat cacaggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    24661 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    24721 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    24781 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    24841 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa
    24901 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    24961 tggttggctc gatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg
    25021 gagccattaa atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt
    25081 attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga
    25141 ttcgagcaaa tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa
    25201 ccaccagttc ctaactggag accaagattt gattttgata aaaaacagtg ggttgagtta
    25261 gctactgaag aagagaagag tggcacagcg gttgacgata ttgaagatgt cgatcagttg
    25321 aagcaattaa atgctctact gacaaaacaa ttggcgatat cggtacagga acaagaaaaa
    25381 atgcaacaaa tgttagctca attgacgatg gaagtcgcaa gtattaagaa tggagggaaa
    25441 agtaatgaat agttttccgg gctttgaaaa tataaaacaa ttttatgatt ggggatgtta
    25501 tacggaccaa gatttacttg actatgtaaa catgaactgt ttaacaaaag accaatataa
    25561 gcagattact ggaaatgaaa tttaattaag cacaaagtta aataaaaagc gtactcaaac
    25621 gagtgcgctt ttttgcataa aggagaaata gatattggaa aaatatttaa atacattctc
    25681 agtaatgaca ggggtgatag gcgggacaat tgttggttta ttgggaggga tggacaatat
    25741 actacatgtt ttaatttttc ttgtgggtgt ggacttctta actggacttg ctaaggcatg
    25801 gaaattgaaa gaaataagta gtgagatagg tttcgaagga ttgttgaaga aagtcttaat
    25861 cttcgttgtg attgccgttg cggttgaagt acagaaaatc gttgggaatt ctatacctct
    25921 aagagaaata gttattatgt tctatgttgc aaatgaaggg attagttttt tagaaaacat
    25981 ttcggtattc cttcctttgc cagataagtt aaaagaggta ttccaacaaa tacgaaatga
    26041 tactgaaaat aaagaccgag gaggaaccaa atgaaaaaga aaatctttgt aggagctatt
    26101 atagctcttt ttttattgcc aataaacgcc tttgcttaca cgatcaacaa tgaattcaat
    26161 ttgggcgcaa acgaaggtag ttctcaagta gcaaataacc aatacatcct attgcatgaa
    26221 acagcaaacg aaactgcgac aggacgaaat gaagcgcagt acatgaaacg ttcttggtac
    26281 aatgcctata cagcgtatat tgttggcgat ggtgggatcg tttaccaagt tggacaacct
    26341 ggttatgtac aatatggcgc tggttcatat gctaatgcaa atagtcctgt gcaaatcgaa
    26401 ctgcaacata cgcacgataa agtaacgttt gaaaagaact acaaggcata cgttgaattg
    26461 gctagagatt cagcaatgaa atatggtatt ccattaacat tagaaacgcc ttataaccaa
    26521 ccaggaatca aatcgcattt atgggtaaca caaaatatct ggggcgatca tacagatcct
    26581 tacggttatc tttctgaaat gggcgtaagt aaagaaaaat tagcatatga tttagctcat
    26641 ggatttaccg atgaaaatcc gacaacttca gatgataaac cagtcattga tccaactaga
    26701 gcaggtgcag caaatcctac actgacagat ggaacaaatt acgcccacat tgatcagttt
    26761 ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc acat
//
LOCUS       DASJTM010000034        26754 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJTM010000034 DASJTM010000000
VERSION     DASJTM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.034"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..1942)
                     /locus_tag="U4B29_001342"
     CDS             complement(11..1942)
                     /locus_tag="U4B29_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2106299.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="U4B29_001343"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="U4B29_001343"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN2106300.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="U4B29_001344"
     CDS             complement(2871..3623)
                     /locus_tag="U4B29_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2106301.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="U4B29_001345"
     CDS             complement(3782..3991)
                     /locus_tag="U4B29_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106302.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="U4B29_001346"
     CDS             complement(4002..5330)
                     /locus_tag="U4B29_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106303.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="U4B29_001347"
     CDS             5466..5621
                     /locus_tag="U4B29_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106304.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /locus_tag="U4B29_001348"
     CDS             complement(5695..6282)
                     /locus_tag="U4B29_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN2106305.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="U4B29_001349"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="U4B29_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN2106306.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /locus_tag="U4B29_001350"
     CDS             complement(7862..9151)
                     /locus_tag="U4B29_001350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN2106307.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="U4B29_001351"
     CDS             complement(9305..10237)
                     /locus_tag="U4B29_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106308.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="U4B29_001352"
     CDS             complement(10394..11053)
                     /locus_tag="U4B29_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2106309.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="U4B29_001353"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="U4B29_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2106310.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="U4B29_001354"
     CDS             complement(12094..12384)
                     /locus_tag="U4B29_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106311.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="U4B29_001355"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="U4B29_001355"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN2106312.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /locus_tag="U4B29_001356"
     CDS             complement(14670..16025)
                     /locus_tag="U4B29_001356"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN2106313.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="U4B29_001357"
     CDS             complement(16049..17218)
                     /locus_tag="U4B29_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106314.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /locus_tag="U4B29_001358"
     CDS             complement(17215..18102)
                     /locus_tag="U4B29_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN2106315.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="U4B29_001359"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="U4B29_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN2106316.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="U4B29_001360"
     CDS             22182..22703
                     /locus_tag="U4B29_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2106317.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23402..23959)
                     /locus_tag="U4B29_001361"
     CDS             complement(23402..23959)
                     /locus_tag="U4B29_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106318.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24138..24749)
                     /locus_tag="U4B29_001362"
     CDS             complement(24138..24749)
                     /locus_tag="U4B29_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106319.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24862..25119)
                     /locus_tag="U4B29_001363"
     CDS             complement(24862..25119)
                     /locus_tag="U4B29_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106320.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25716..25994)
                     /locus_tag="U4B29_001364"
     CDS             complement(25716..25994)
                     /locus_tag="U4B29_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106321.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26028..26279)
                     /locus_tag="U4B29_001365"
     CDS             complement(26028..26279)
                     /locus_tag="U4B29_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106322.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7554 a         5760 c         4581 g         8859 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtgg gacaaaagta aagaacactt
    22741 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcttatttct
    22801 tggagttaaa cgcttctgtc ccagcctcct tattccgaat aaatggtgtc caaaagcttg
    22861 catctgccag attatctcat ttcttatgac aagctaagca aattatctcc accaaataat
    22921 ttattcatat ttttgacttg cgcatactaa ctaattctta aattttcata caaaatttct
    22981 caatttccct taacgagtaa tatttatgta cggtgattct cactagtatc ccaatcactt
    23041 tttaagttat cccgatttca attctacttc aaaaatagtt gcttttatgt atgctgttag
    23101 ttgattagat ggtcatttgt tgagctaggt cgtttatttt aaacaagttg tttctttagc
    23161 ttatacctat ctcgtctacc aaacgcaaga tggcaaacac aaatgcatac gcatctgtat
    23221 ttgccacttt gattcgtata aagctaacaa ctattaactc ctctcagcta atagttgctc
    23281 tcttttttat tcagattcat ttttgctgat cacagcctag tatttcaatg gttttgaaca
    23341 ctaaaccaaa ttgttccatt acaacattca ctggctgatg aaatggatta gtagatttcg
    23401 attattcaaa gcgattttct attggtggat cgtgttccca actaataaga cggattccaa
    23461 acctatcgta cagatcttct aaaccactta taaattttgc acctatttca ggtgttttat
    23521 tgcgtggata ttctattgtt acgccattaa atggatacca attaggatta ggctctcttt
    23581 ttttctgtac atctcccata gaatagttag cactttctgg gacataatat attcttcgac
    23641 catcctctaa ttcatctaat aagatatttc gtgatcgtct atcacttgct aaatgtagac
    23701 gccacgtgcg ataccatgcc atgctacctg catctgcaga aaatccatca aataatgcaa
    23761 caattgtatc ttctatcaca atcctttccc catctacttc atattctaat ctaaaatcaa
    23821 actcccctcg ggtaatttct ggtcttggtg gcttaggacc caatattatt ctacttaaaa
    23881 ccgcattcgt tcccagatga aacacagcta taacaacaat aatgattaat attttgtttc
    23941 tatttgataa atttctcatt attcgcacat cacctctaca ttctcacata ttagttcgta
    24001 atactccttt aatatcaaaa tatctactgg tcgtaataat attcgctatg aattcctact
    24061 actcgtataa gaaaggccaa atcatcagct tttacttgct gatgatttgg cttgtttcta
    24121 tttatcgtaa ttgttttcta atcaccaaaa ttgttaacaa tcggttcaga tatttcccag
    24181 ttgattaagg taattccgta ttgtgtcaaa acttcttgcg cttctccgaa attaagtaca
    24241 gttcttatgt atccactttc tcctaatata aagtctacct ctcttttaaa accttcctcg
    24301 agatttctaa gggctatatg tggttttaat gacattgatt gtacattttc ccccatataa
    24361 tattgcgcac ttcctcgact aaaatagatt cctagatcgt ccccaaaagg tagatcttct
    24421 ctaccactag ctaatctact tctccatctt cgttctcttt tcatagaacc tgcgctgaac
    24481 tcacacccgt taaactcagc tatgacagta tcttcaatta tgttaatctg tccatctagt
    24541 tcgtattcaa tcctaaatgg aaattctcca taggtgattt ccggttcggg tattttaggt
    24601 gagattaatg caaagaaagg aaatattaag cttggcaaca tcaaaaaaac tcctcctatg
    24661 atcacaattc ccgatgtaat ccaaaatata atttgtacaa ttctcattga tccattgtat
    24721 tccattttat tccccctatt actattcata tccgcctatc tatatcgatc ttccaatgca
    24781 gagataattg cttccatacc ttgatttttt actgagttac ctatgcgaga tatcattctc
    24841 cactaaggtg catcatattg tctatctaac atgattaatt ctccaatagg tgtatactcc
    24901 tccaaaaaca acatttaatg catctccctc gacaacataa gctaccatgt ttgctgtata
    24961 tgtgtttgag tctaattcat aagcagataa atttactgtc gctggattaa atataatcgc
    25021 attcgtattt atagctactg aatttgctgc ttcagcacca ccttttgaat gtccgatcat
    25081 tgtgatttca atagtaggat ttacgtggac agccctcacc gtttaccctg accattatag
    25141 aaatcgctta cttttttgaa ctacgcaaca aaggatacaa tgctcctaac ttcactgtct
    25201 ccatttttgt ccactaagga atataaaaaa taggcattaa ttcatttaat tcggtcacaa
    25261 cacgttacct tttaaatcat tatactgtat ctcatacatt attatcaata aactattata
    25321 aaaatgcaaa aaatgcttta tcaatctatt ttgtaattgg tcataatatc attgaaatca
    25381 taccctgtat cgatttttcc tatgaatctc gtctatttgt gattgacgaa aaagaggcaa
    25441 gacagacact ggaatggtaa ttttacccac tattttgctc caactatttg taatcgtacc
    25501 cacttttgaa ataccgagat gatcatttta attgatatgc tgattcccaa gcaaaacccc
    25561 aagaaattaa atgctctatc gcatctagct tcttgggttt tgcttatata aattccaaca
    25621 tttaaatttt ttatgctgtt cggtctcaga aatgccgttt ttgcttatat cacaatatca
    25681 agatgtcaaa ttattcgtgt tgccgatgac tttgattaat ttttttcaaa ttgtttatta
    25741 agactaatgc tgtctttttg cagataccaa tgttcgaact ttttaacatt atcatatgat
    25801 cctgcaagtt ttaacttctt accattagat gttatcaaag ttataattaa ccttgccttt
    25861 ttcccattag gatcttttat tttatgaatt ttctgcaggt aatctctcct aatggcagct
    25921 atttttacct catcagtgct gataaaccat tgatcatcca catacatact gcctgttatg
    25981 ttgtgttttt ccatttcttc cgagaacgaa aagtttaagg ctcgttctta cttccaaagt
    26041 tgaatgagtc catggattgg acttagtgtg agaattgaga acaacaataa gaatccatat
    26101 cctcccgcaa acatccctaa ggctatactg tctctccatc cataagtagg attagaagag
    26161 aaaagagtca ccgtttgtaa taatatgaaa aggaataatc cagcacaaac accaaagata
    26221 acaactccaa acattgaatt tttatacaat gaccatttat ttataggctt ttctttcatt
    26281 ttacttcccc ctactgttct gttcacacgg aatgaattca aagttcataa gccctcctct
    26341 ttgaacatat gaatacgact tgctaactat actttaaaaa agcttgtttt aagcggttat
    26401 aggcgagttg taatctctat tcatctcatt agcaaataac ttgccgaaaa gtctactgaa
    26461 attaaagcag atagtggaag aattatttag ccaggataga cactatttgt gaaacacatg
    26521 tctttacttt tgaaattcta tgatgaaaag ccttggaata cagtagctct taattggtaa
    26581 tactttttcc attataaata acctcttttc attttatatt tatcccctct aaaaataata
    26641 tctaatctaa tattttaaac ttatatttcc ctcgctgtgc taatagagca ttactaaata
    26701 aatagaaact agaaaaaagc attacgcctg ccagaataaa ccaattattt gtat
//
LOCUS       DASJTM010000035        26509 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJTM010000035 DASJTM010000000
VERSION     DASJTM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.035"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..401
                     /locus_tag="U4B29_001366"
     CDS             24..401
                     /locus_tag="U4B29_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106323.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            970..2358
                     /gene="ltrA"
                     /locus_tag="U4B29_001367"
     CDS             970..2358
                     /gene="ltrA"
                     /locus_tag="U4B29_001367"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2106324.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2480..3775)
                     /gene="purB"
                     /locus_tag="U4B29_001368"
     CDS             complement(2480..3775)
                     /gene="purB"
                     /locus_tag="U4B29_001368"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN2106325.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3788..4912)
                     /gene="purK"
                     /locus_tag="U4B29_001369"
     CDS             complement(3788..4912)
                     /gene="purK"
                     /locus_tag="U4B29_001369"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN2106326.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4905..5393)
                     /gene="purE"
                     /locus_tag="U4B29_001370"
     CDS             complement(4905..5393)
                     /gene="purE"
                     /locus_tag="U4B29_001370"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN2106327.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5809..7116)
                     /locus_tag="U4B29_001371"
     CDS             complement(5809..7116)
                     /locus_tag="U4B29_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN2106328.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(7119..7700)
                     /locus_tag="U4B29_001372"
     CDS             complement(7119..7700)
                     /locus_tag="U4B29_001372"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN2106329.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7816..7913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(8272..9564)
                     /locus_tag="U4B29_001373"
     CDS             complement(8272..9564)
                     /locus_tag="U4B29_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2106330.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(9561..10184)
                     /locus_tag="U4B29_001374"
     CDS             complement(9561..10184)
                     /locus_tag="U4B29_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106331.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(10187..11023)
                     /locus_tag="U4B29_001375"
     CDS             complement(10187..11023)
                     /locus_tag="U4B29_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2106332.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(11040..11927)
                     /locus_tag="U4B29_001376"
     CDS             complement(11040..11927)
                     /locus_tag="U4B29_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2106333.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(11943..13226)
                     /locus_tag="U4B29_001377"
     CDS             complement(11943..13226)
                     /locus_tag="U4B29_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2106334.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(13909..14592)
                     /locus_tag="U4B29_001378"
     CDS             complement(13909..14592)
                     /locus_tag="U4B29_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2106335.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(14610..15227)
                     /locus_tag="U4B29_001379"
     CDS             complement(14610..15227)
                     /locus_tag="U4B29_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2106336.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(15237..16061)
                     /locus_tag="U4B29_001380"
     CDS             complement(15237..16061)
                     /locus_tag="U4B29_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN2106337.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(16054..16614)
                     /locus_tag="U4B29_001381"
     CDS             complement(16054..16614)
                     /locus_tag="U4B29_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106338.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16788..17744
                     /locus_tag="U4B29_001382"
     CDS             16788..17744
                     /locus_tag="U4B29_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2106339.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(18049..19005)
                     /locus_tag="U4B29_001383"
     CDS             complement(18049..19005)
                     /locus_tag="U4B29_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN2106340.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            19109..19711
                     /locus_tag="U4B29_001384"
     CDS             19109..19711
                     /locus_tag="U4B29_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2106341.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(19799..21298)
                     /locus_tag="U4B29_001385"
     CDS             complement(19799..21298)
                     /locus_tag="U4B29_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN2106342.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(21314..21838)
                     /locus_tag="U4B29_001386"
     CDS             complement(21314..21838)
                     /locus_tag="U4B29_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN2106343.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            22136..22885
                     /locus_tag="U4B29_001387"
     CDS             22136..22885
                     /locus_tag="U4B29_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2106344.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            22876..23661
                     /locus_tag="U4B29_001388"
     CDS             22876..23661
                     /locus_tag="U4B29_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN2106345.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            23630..24346
                     /locus_tag="U4B29_001389"
     CDS             23630..24346
                     /locus_tag="U4B29_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2106346.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(24448..25482)
                     /locus_tag="U4B29_001390"
     CDS             complement(24448..25482)
                     /locus_tag="U4B29_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN2106347.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(25486..>26509)
                     /locus_tag="U4B29_001391"
     CDS             complement(25486..>26509)
                     /locus_tag="U4B29_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN2106348.1"
                     /translation="DMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVAL
                     PALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTI
                     FGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFV
                     NPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLS
                     SPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNF
                     EALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIH
                     GIGLFS"
BASE COUNT         7959 a         5653 c         4758 g         8139 t
ORIGIN      
        1 tgggttcgag gaggcacttg ccgatggaag atgcaataaa acaaggtcag ctttttttat
       61 cagaattaac agctgaagaa cgttcctgtc tatataatga tttgctgtcg atcaaagacg
      121 tttatttatg caataaagat actagttttt atacattccg aaaaatccgc cgagcaacca
      181 agcgctgtgc tgaatgccgt aaaaggactt ttctttatca gacagaacac aaaggcgaac
      241 tttcaatcag tcagattcat gaacagaagc gtttcaaaaa tcattatcgt atccagatct
      301 tattcaatgg acaagagatg gaatacgctt ttctcgaaaa cttgctgcaa catctccata
      361 accagcattt tagttataat agccaagcca tttacgctta attcctctat tttttgtatg
      421 taaagaggca cggtaaagag gtagccgctt ttaaaatcag gttcctatta ttgataagaa
      481 aatataataa actaaaagaa gcagaaccgc taacggatct gcttcttttt tggtgtgcgc
      541 cctgcatggg tgaaaacttg gtggtgaaag tccactacag gcttggcagt aggaactgtt
      601 agcgaaagac aaggcggcaa tcgtgaggtt gtggctgaag gaagtcggac gcaaactcct
      661 gaactgacga acagaaacta gatacaaggc tgaattaggt cggataagtc ttcatcacaa
      721 gacaaagtcc aatactgccc gaaacctata cagtaaatct agcagttaca tgggaggaaa
      781 gttttcactc ttaccggggg agatctcatg agggtgttgt gggcgttgaa tgaaaacaaa
      841 ccagcacaac gatcatggcg acatgatggt gaatcatgag aagtcagccg aggtcatagt
      901 agtaacgaca ctgaccgtta cgaaggactg aacaataaga atcttgaata atttaggagg
      961 tgtgagaaga tgcgacgacc gcagaaaaca agaaaacttg gctatcatca aagagctagt
     1021 gtggaacacg aagggtatga tgaagtgcgc agcacttctt gcggtgaaca gaggaaacaa
     1081 gatggtgtag aaaatctgtt tgggagaatt ctagatcgga ataatctcaa tcaagcgtat
     1141 cttcaagtag tcagaaataa aggggcggct ggtgcagatg ggatgaccgt cgatcaactg
     1201 cttccttact tgaaagagca ccgcgaggaa ttactatcca atttacgcca agggaactat
     1261 aaacctcagc ctgttttgcg agtagaaata ccgaaaccaa acggcggtgt aagaaaacta
     1321 ggaattccca cggttattga tcgtatgctt caacaggcaa tcaatcaagt gttacaaccg
     1381 ctatttgaac cagaattctc agataatagt tatgggtttc gcccaaatcg cagcgcacat
     1441 gacgcagtga agaaagcaaa atcttattat gaacaaggtt accgatatgt ggtggacatt
     1501 gatatgaaag cctactttga caccgtaaat cacgataagc tgatgtattt cattgagcgg
     1561 aaagtaaagg acaaacaagt cctaaaactg atccgtcaat acttaatgag tggtgtaatg
     1621 gaaaatggac tcatcacaac aactgaagaa ggaacgcccc aaggaggcaa tctctctccc
     1681 ctgttaagta acatctacct gaacgaattt gatcaagtgc tagaaaatct aggacataaa
     1741 tttgttcgat acgccgatga ctgcaatatc tatgttagaa gcaaacgagc aggacagcgt
     1801 gtgatgaaga aatccattca atttctcgaa gaacaactaa agttgacggt aaatcgagaa
     1861 aagagtgcgg taggaagtcc attgaaacga aagtttcttg gattctgcat tctgccaaca
     1921 aagaaaggta ttaaaattcg ccctcacgca aaagcaaaac agcgtgtgaa aagcaagctc
     1981 aaacaactca ctaaaagaaa ccgtggaaga agtatccaac ttattttaaa agaaatcaag
     2041 caactaatga ctggttggat caactactat ggaattggtg agatgaaaga attcatgagg
     2101 gagttaaacg gttggttgaa gcgaagaatc agacaatata tttggaaaca atggaagaat
     2161 ccaagaacca aaagaaaaaa tctgattcgt ttagggatcg ataaagcgaa agcgtatgaa
     2221 tggtcaaata caagaaaagg tatttggtca gtctccaaaa gtcatattct tcatcgatca
     2281 ttaacagaca aagaactggc ccgccaagga tatgaggaca tctccttgaa gtaccagttc
     2341 gtacactcaa cttattgaac cgccgtatac gggtccgtac gtacggtggt gtgagagggg
     2401 cggaaggtca gttggctttc ccctctactc gattattttt gctattctat tttaattgaa
     2461 ttttgcaact cactcttctt tattccaaac ctactcgttt gaatatcacg tcaacgtttt
     2521 tcaaatgatg atgataatca aaggcatcgt ctatttcttc tttagataat accgatgtga
     2581 tggttggatc ttcttctaac agcgggcgga actgcacttg gttgtcccaa gcgtacgcag
     2641 tttttggctg aacaagatca taagcttctt ctcttgtcat tcccttatcg atcaatttca
     2701 gcataacgcg ctggctataa atcaaaccaa atgttgcaga catattccgc ttcatgtttt
     2761 caggaaagac cgttagattt ttaacgatat tgccaaaacg atttaacatg taattcaata
     2821 aaatcgtggt atcaggcaag atgattcgtt ctgctgaaga atgagaaata tctctttcat
     2881 gccataatgt gacattttca taggcagtga ccatgtgtcc gcgcaccact ctggctagac
     2941 cagccatatt ttctgaaccg atcggattcc ttttgtgcgg catagcagaa gaaccttttt
     3001 gacctttcgc aaaaaattct tctacttcac gtgtttcgga tttttgcaat ccgcgtatct
     3061 ctgttgcaaa acgttcaata cttgtagcaa taagcgccat cgctgagaaa tattcagcat
     3121 gtagatcccg agggagaact tgagtagaga tttcttgtgg acggatcccc aattgtttac
     3181 atacatactc ttcaataaat ggcgggatat ttgcgaacgt tcccactgct cctgagattt
     3241 ttcctgcttc tactcctttt gccgcatgtt caaatcgttc gatgttccgt ttcatttctg
     3301 aataccatgt ggcaagtttc aatccaaaag tagttggttc agcatggaca ccgtgtgtcc
     3361 gacccatcat cactgtatat ttgtgttctt ttgctttttc gccaatgatt tccgtaaaac
     3421 gtttcaagtc ttcccgtaaa atatcatttg cttgcttgat gagatagcca taagcagtat
     3481 ctacgacatc cgtacttgtt agaccataat ggacccattt tctttcttct cccaatgttt
     3541 cagaaaccgc tcgtgtaaac gcaacgacat catggcgtgt taatttttcg atttccaaga
     3601 tccgatcgat atcaaatgat gcatgctcac gaattttttt cacatcttct tgcgggattt
     3661 caccaagttc tgcccaagct tcatctgcta aaatctcgac ttcgagccac gcacggtatt
     3721 tgttctcttc cgtccagata ttccccattt cagggcgtgt ataacgttct aacatgtttt
     3781 ttctcctcta atcccaaata ttcgtttgtt tgatttcttc cagcgtttgc tggatgtctt
     3841 ctgtcaagat agtgatatgt cccatcttgc gacctttttt tgcttgtttt tttccataga
     3901 aatggaattg ccagtctggc ttattctcaa tcaagcgata gctttccagc agttgttctc
     3961 ccaaaacatt gaccattact gcattgctta tcaaacgtac tttttctcct aatggccaac
     4021 cacaaatacc tcgaatatgg gcatcgaact gactcataga acaagcttca atagaataat
     4081 gtccagaatt gtgaggacga ggagctaatt cattcacata tagcccgcct gtttttgtta
     4141 aaaacatttc tacccctaaa acacctgaaa ggctcactgc ttctgcaatc acacgggcaa
     4201 tacgctcagc ctcctcgatc acatcctgat caatcgcggc tggagcaatc gtttcatgaa
     4261 gaatattgtt gtggtggatg ttttccacga ccggaaaagt cgtgaaatct ccttgaccat
     4321 ttcctgccac catgatcgaa atctcttttt caaacgggat ccatgcttct aaaacacagg
     4381 ttccttctct taaaaggtcc atggcaggag ctagatccgc ccgacttttc aaaacatact
     4441 gccccttgcc atcatagccg cctcgagtgg ttttcaaaac acaaggatag ccgatcccgt
     4501 caatcgcatc ttggatatcc gtcggactga cgatcgttgc ataaggagca atcacgatgt
     4561 tattcgtttc caaaaaagat ttttctaaca ggcggtcttg cgtaatcgca agcaagtctg
     4621 tcccttgagg aataaatgac atgggtaaaa tcgcatttaa cgcctctaca ctgacgtttt
     4681 cgaattcata ggtaatgaca tctgttcttc tggcaagctc tttcagcgca aaggtgtcat
     4741 catagacact ttcaatgtgc caatctgcaa cttgtgcggc tggacaatcg ctcacaggat
     4801 ccagcacacc tactttaaag cccatttctt ttgcactaat cgccatcata cgacctaatt
     4861 gaccgccacc gacgatcccg atcgttgaac caggcaacaa aggtttaacc aagttgatca
     4921 ctactttcta taacagtttt agccaacatt gaacgtcttt cagccaattt agcctccaat
     4981 tctaaatcat acatggaaag catctgtatc gcaagtagtc cagcattaat tgctcccgct
     5041 tttccgattg ctgtagtagc tacaggtact cctccaggca tttggacaat cgaaagaagc
     5101 gaatccaaac catttaatgt tcgtgattgg accggtacac cgataaccgg caaagttgtt
     5161 tttgatgcga ccatacccgg cagatgcgcg gctccccctg ctcctgcaat aatgattttg
     5221 attccttttt ttcgagcgtt ttgtgcatat tcaaacatca aatctggggt tcgatgagca
     5281 gaaacgacct ttttttcata agacacaccg aattcatcca atatttgaca tgcttctttc
     5341 attgtttccc aatctgaagt actccccata atcacagaga tttttgctgt cattgttttt
     5401 cctcccagtt tttctacatt catattttaa caatcacttt ttttgatgtc aaagaaattt
     5461 cgaatgttaa caaagaaaaa cattttattg ttcgtgttta ttaaagataa aaaaaaggaa
     5521 tgtgacaaaa gttaggtcac attccttttt atggataaac ggtgttctcc tgccaactcc
     5581 tcggtcacaa ggaacaatat tcaacaaaca tgagaagctt ttttttgaaa ttgttccttg
     5641 tgactcggag atgaacggca ggctcacgac ctcttccttc aacggtgttc aatcagctga
     5701 ccctcggtat tagctcaaac aaacggcaaa atacgaggaa ctatttttcc gtttgtttct
     5761 ctaacaatca ggtcaaaacg ctgatttcac aacctttctt ttgctctatt acttcttgaa
     5821 ggcttcttcc aatatttctt catctttttt caatccatta aagatcaaat tcaatatgat
     5881 agccgtaatg ctactcatta cgatgccatt tccagtgaac atttgcaatg tttgcggcat
     5941 ttgactgaat aatgtcggcg taacattgaa acccagccca aatccaacag aaacagcaac
     6001 aatcaataga tttttatcat tgctgaaatc aacttccaac agcatacgga ttccttggac
     6061 tgccaccatc ccgaacatga ctaacattcc tccaccaagt accggctcag ggatgatttg
     6121 tgctaacgca ccgattttag ggaataatcc taaaacaatc aagaagaatg cagagaaata
     6181 gatgggtttt cttgttttga ttcccgaaag ttgaaccaat ccgacatttt gagaaaagcc
     6241 agtataaggg aatgtattga aaataccgcc taagataacg gcaagtcctt ctgcacgata
     6301 tccctttttc aattcttctt ctccaacatt tttcccagta atgtcaccaa gtgcaaaata
     6361 tacacctgtt gattctacca tgctgaccat ggaaatgatg atcattagta aaatcgacca
     6421 tacgtcgaaa gttggtttac caaaataaaa gggttgtgga acatggaaca acggcgcatc
     6481 tccaatcgcg ctcagatcaa tcattcccat gaaagcagct aagatgcttc ctccgactaa
     6541 accaatcaat accgcgattg aacgaatgaa cccttttgcc agcatttgca cgcctacaat
     6601 taggagaatc gtcacaaatg caagtagcag atttgttttg tcaccaaaat cagctgctgt
     6661 tgcattacct ccacccattt tctcgattgc tacaggaatc aaagtcaaac caataacggt
     6721 gataaccgtt cctgttacta taggagggaa tagtttttta attttagaaa agacaccaga
     6781 aactaacaca acaaagatcc cagaagcgat aatagatccg tagatagctc ctactccttc
     6841 attggttccg atcaagatca gtggagctac tgcttggatc gcacatccta aaacgacagg
     6901 caagccaatt ccgaaaaatc ggttaactgt caattgcaat aatgtcgcta atccacacat
     6961 aaagatatca attgaaatca aataagtcat ctgttcttga ttaaagccta gtcctgtacc
     7021 gatcaaaaga ggcaccgcta ctgctcctgc atacatcgct agcagatgct gcagccctaa
     7081 aaccgcagct tttccatttt gtgtttcttt ttccacgatt atgcgtcctc ctcaagaaac
     7141 tcaacttgtc cattcgtcaa ggaagcaata cgcgcaagag aaacgactct caatcccatt
     7201 tcttcaagta actgacgtcc atcttggaaa gacttttcga tgacgatccc gattccttct
     7261 acttgcgctc cagcttgctg gcataattcg atcaatcctt ttgccgcttg tccgtttgcc
     7321 aagaaatcgt cgataatcaa tactttgtct tctgaagata aaaatttacg agaaatagaa
     7381 atcgtacttg tcacttgttt cgtaaaagaa taaacagaag ctgtcaataa ttcttcatcc
     7441 atcgtcaaac tttttgcttt tctagcgaag atcattggaa catctaattc tcttgcagca
     7501 aataaagctg gtgcgatccc agaagcttca atggtcacaa ctttcgtgat tccttgttct
     7561 ttaaatactt ctgcaaaccg ttttcccatt tgttccatca atacagggtc tacttggtga
     7621 gtgacgaagc tgtcgacttt taatacgccc tcgcctaata tacgtccgtc tttttgaata
     7681 cgttcaacta attctttcac tttatttgct cctttatcat ttattaatac cgaaataacc
     7741 gcttttgtgc aaaaacctac tatgtttctg ttaaagaaaa acaccctcca ctttatcata
     7801 agaaaagaag ggtgaaatag ccaatctttt acttatagtc cagcatttac ggtactggct
     7861 agaaactgtc atccatatta tgacgatata taagtaggta tgtaaaacta ataatagtgt
     7921 aaattcattt tcttgcaact gttttgcacg tgaaggcagt ttgccatatt tgaataaaaa
     7981 atgttgtttc aaatattgaa aacagtctaa cactactttc tgaaactgac aacgtggttt
     8041 acttattttt ttgtaaaatc taatgatttt taatgcatta ttcctttttg ttcgggttta
     8101 aatagttatt tttattttct cctatctaaa aaagcagccc acataaaaag atggcatgac
     8161 aaaaagcatg tcatagcccc tttttccgaa taaatagtgt tcaaaagaca gcctatcggc
     8221 aataaggcaa gattttataa actacgggaa gtaatttccg taaatctatt cttattcttt
     8281 taagctgaca agctcgtcaa tcactactgc ttttcctgaa gcaatagaat gatttgctgc
     8341 gattcctgtc aaaatcgaca aaataccatc tacgtgagtt gctgcccgat tccacggatc
     8401 ttcttctttt tcttcaccaa atagatcatc taataaacgc ggatctcctc cgccatgtcc
     8461 gcccgcttct tgttcgacga ttacctcata tggctcaccg aacatcggat agacatggat
     8521 ggactgaaga acggctgctc cttcatcctt tttatctcca cctgcattga tgtaagactt
     8581 ttcgatcact ttcatttcga tccgtccttt gctgccattg aaagaaacat tgaatccttc
     8641 ccacggcata tacgcgttta gcgaatacgt caaaatcgca ccagtttcat atttgaccgt
     8701 tacgcctagt gtatcttcaa tagagatatc atcggaaaat acactgcggt cacgaatata
     8761 tccactgtct ttttctgctt ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc
     8821 tatagcaaat ggatcagctt tagctagttc gcttccatag catcgactgt aaaatatttc
     8881 tactccgcgt ttctccgcat tatgcatccc ataaaagttt aatccaccga atgcaaatac
     8941 cttttgggga cggctgttca accaaaaatt cactagatca aaatgatggg tagatttatg
     9001 aacaagaagc ccgccgctat tgtgcttatt tcgatgccat cttctgaaat aatctgcccc
     9061 gtgttgcgta tttaacagcc attcgaagtg gatagaaaac acatcaccaa tcacatcact
     9121 ttctatcagt tcctttatct tcgtgtgata cggtgcataa cgataattaa aagtgactcg
     9181 caggcttctt cccgtctgtt tttggaccgt tattatttct tgagcttttt cctcatcgat
     9241 agtcagaggt ttttcgcaaa taacatcata tccttttttc attgcacgaa tgatataatc
     9301 atgatgcgtt cgatctactg acgtaataat gacttcagcc ggccgtacgt catccagtag
     9361 ttgctcaaat tgtgatggca tacatgtctt tacttttttc tgtccatact tgttcacaag
     9421 tatccgattt gcataatcca tccttgtttg agatacatca caaaaaccga ctagttcaca
     9481 agtttgatga tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc
     9541 gacttgtaca tattttttct tcatttaaag tcattctcct tttcttttaa gcaaaatact
     9601 gtgacaaatg gcagccctgg atacagtaat gcagtgagta aaaaatcatg ctgtatacac
     9661 cagaacagtc catacaaaag gcataaatac cacagaaacc ccttgacgtt ttttgacaac
     9721 agccagatat tccttttgat caaataaaca agactatctg ctttatcttc tttgcagata
     9781 tcaattagcg tactaaaaaa actatgggat acaaaaagca acagacacaa tagaaaagga
     9841 atcagatacc aagctccaat ctttcccact aataaatacc aatcaactgc aaaaataatg
     9901 acaaacagct gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc tttcctccaa
     9961 catacctttt tttctgccaa gtaatggaca gatagataat aagaaggata catcatcaaa
    10021 aaataaatca gatagacaac acgagcagac agcactaatc ctaaagtgaa caaacatcct
    10081 agaactaaag cgaaaagagc attactgata agactggtta aaaaaatccg acagacatag
    10141 tagttgagct tgtaaagaaa catctcttct attggctttt tcatattcag cccttcagac
    10201 cgcttgtact gatcccctgg acaagatatt tattgaagaa taagaaaatc agaaaaaccg
    10261 gtagcagcga caacgtagac atcgcaaaca tactcgcgta agtcgtacta gatccagcgt
    10321 ctgcaaataa tttgatggca atcgataccg tgtaagatct cggcttgttt aaataaatca
    10381 atggtcccat gaagtcgtcc catttccaat aaaactggat gatgatcgtc gtgatgatcg
    10441 aaggtttcag caaaggaaaa ataattttgg taaagacact atacttgctg catccgtcaa
    10501 taatcgctga ttcatctaac tctttgggga tgctgaccat gaattgcatg atctggtaaa
    10561 tgaaaaatgc ttgtccaaaa aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat
    10621 tcagccgatt gtaaataata aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg
    10681 ttactaacat gacgacgaac cagaatcttt ttcctttgaa atcgattctc gataatgcgt
    10741 aagcaataca agtcgagctc agaactgtcc ctatcgtcga aactgcagag atgacaaacg
    10801 aattcctaaa gaaagtaaaa aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat
    10861 tttcccattg aaaagtctct ggaatcaaat tcagcgaacc actcataatc tcttgatccg
    10921 ttttaaaaga acctgcgatc atccataaga ccggataaat catgactaga cctaatagca
    10981 aaataaaacc gtattgaaac catttcgtta ctgtattatt catttcttcc tcctctcatt
    11041 cacacaacga cccatttttt tgatgatttg aaaatcagaa aggtgacagc tcccataatc
    11101 aacagcatga tccacgaaat agcactagca taccccatag aattaaaaga aaaagcttgg
    11161 ttatacacat aaagagcata aaagttcgtg gcatcattcg gaccaccttg ggtaatgata
    11221 aatgcctgcg taaaagtcat aaacgccgcg attgtctgca taaccaagtt atacaaaatg
    11281 atcggcgaaa gaagaggtaa cgtgatcttg taaaacgtct gccatttgtt cgctccatcg
    11341 atctctgccg cttcataata cgagcctggg atctctttga tacctgcagc aaagatgatc
    11401 attgccgaac cgaattgcca agcagacatc aaaacaagag ggatcatgac cagtttttga
    11461 tcgctaaacc aggaaagcgc aggtgcgcct aatcgttcca ggaaaatatt cactaacccg
    11521 tttttagaaa acaactcccg ccaaactaga gcgatagaaa tactgccacc gatcaaacta
    11581 ggcacataat acaaagaacg aaagaaagtc gttcctttga tattcctcgt taaaagataa
    11641 gcaatcaata gaccaaagat cagtttcaca ggtacagatg caaatacata gagaaaagtg
    11701 actcggatgg agttcaaaaa acgggcatca ctcagcatct ttatataatt atccaagccg
    11761 atccatgaga ctggatttcc caaacgataa tcggtgaacg aataataaag tgacaagatc
    11821 atcggtatca ctgttatcaa tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg
    11881 accacattgt cttgatccaa gaaccgtttt agtttttctt ttttcatgcc ttcctcctgc
    11941 tgctatccta gaatgtttga cgcaaactca taaagactct ctgctgcttc ttctgctgtt
    12001 acttcttcat aaatgacttt ttcaactaaa agattgtatt ggtcttgaat ttccggattc
    12061 aaagcactat caaagacatt cacttcgccg ttgtccatat cggaaatcat ttctaaataa
    12121 tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga gctgttctcg aatattcgac
    12181 atgattggta caccacgttc tccttttaaa atttcattgg cttcttcact gttttgaaag
    12241 aaatcaataa acttagcagc ttcttcttta tgggctgagc tttttgccat actcagcatt
    12301 tgcgaagaac gtaaagggat accagattct ccatctgctt ttctgcgagg tggattaatc
    12361 aactgaatct ccatatcttc aggagcccct gacaatattt ctgtcaactg gttacttgat
    12421 agaaacacca ttgccgcttc tctatcccta accgcctgaa aagacagatc cgtcgtactt
    12481 gcagctccac cacctggttc tggatgggcc cctttgtcta ccagattttt acgcagctgg
    12541 aaaaaatctg ttagatagtc tggattttca aatcccaagc tgttagatag atcttcccga
    12601 ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa taatacagcc agctataaaa
    12661 tcttcgaatc ttgaaatagc atagattcct aattcttcag tgatttgata gctgatttgt
    12721 tcaaattcat cccatgtcca atttgaagct ggttcaggga tatttaattg ttcaaatata
    12781 tttttattgt aagcaatcgc aaacgaattc accccgttag acagtccaac taattgcccg
    12841 tcgtaagtcg tcgttgctaa ataggattca tctgcattag aaatatcaat tatcccttgc
    12901 tccacatatt catctaatgg ttcaatctga ttgatgtatt gaggaaattg attgttcagt
    12961 tggaaaatgt cccagatatc atctgatgct gctagtgtac ctaattttgt gtaatatcct
    13021 tcataatcat aaaattcata atcgatcgtc acatggggat ttttttcttc ataaagctcg
    13081 attactttta ctgttgcatc gtgtcggact tgcgaacccc accaagccat gttcagcgtg
    13141 atttttttgt catcttcttg cccacaagcc gtcaagaaga ataacataac acttaaagct
    13201 aagatgattc tcattctttt cttcattctc tgcacctcta cttccattgt tatttttcaa
    13261 tatactgtat ttttatacag aacatattta ttaaacgatc tttttcaagg ggaataaaaa
    13321 ccaagacttg ccttatcgtt ctgcctagga tgaacaactc aatactttca agaaaaatta
    13381 gatagtgctg attcttcttt catcgctaaa aacacttctt gcttcaatga tgggaggata
    13441 attacgcaaa gaaaaaagga tatcagacaa ctgcccaata tccttgacat agctaaataa
    13501 gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt aattctgtat
    13561 gaaattaggt acaatataca cgtcgatgca caagcattga ctatggcaac ttgattggtc
    13621 agttcccgct gacttttcaa gccccgacaa tttagctgct actaaattgt cgggtgcctt
    13681 ttttagtttt tattcgtttt tcattctata gaaagcgctt caataaatca agacattttt
    13741 ttgttagaat gctaataata acaaaaggtt ttaactcttc ttatgtacat tacaggtaaa
    13801 aaaacaaaaa aatttctctt ttttttgaat tctgaacaaa tagtactgga gtacaaaaga
    13861 cgaggaagag ccactatctg ctcttcctcg tcttttgatt ctcacctgtt attcatgatt
    13921 ctttttctca gtttcccgat atttttccat tttacgacta aaaagctctc cgctacttgg
    13981 tggcgtatat gtgatggcat ttgctcctgc ttgaatggtc tcagcaatac tttcttccgt
    14041 tggaccacct gtcgcaatga taggaagttc aggatatttt ttacgaaaat ggcgtactgc
    14101 ttctgcagtt tctttacctg cactgatatt gataatcgaa ataccagctg ctaacttttc
    14161 atctatttct gtatatttcg acgttaccgt actgataact ggaatatcaa ctactcgaca
    14221 aacttctttt actgtctcta aaggcgtagg accgttcaaa acgaccccta acgaaccttg
    14281 cgcttctgcg aacatgctca tgtaggaaga acgagcacct tgtgtcaaac caccacctac
    14341 accggagaaa actggaatgt cagctgcctc aataatgctt ttagtaatcg ctggatgagg
    14401 tgtaaaagga tacacagcaa ttactgcatt cgcatttgta ttacgtatga tcgcaatatc
    14461 cgtagtaaag atgatcgaac ggatcaattt tccaaatatc ttgatcccgc ttgcttctaa
    14521 aatgatttcc ggtactctta cgatatcttt tctcaattcc gtcatgattt ctggcgtaaa
    14581 ttgttctgcc atcagccact tctcctttgc tatctccggt catactgatt caattgatac
    14641 gtctcgatga cctggatgat tttttgcgca taaccggggt ctgtcgcata acctgcatct
    14701 tgcaatgctt gtgcggcttc ctgatagttg gtggccgtaa tcactttttc atacagcgca
    14761 ggattccaat ctactccttg gacgaacagt tgtgtatggt catccatgga ctgttcccat
    14821 gaatcataca ctttgaagtc tccttggata gttatccatt cttcgttaac aaattccttt
    14881 gtttccaggc ttactttctt ctgatcccca taagccttga tcccgaaaag attattgtat
    14941 ttgctagcca aggtactttt tccccaattt gattctaata tagcttgtcc taaaatgata
    15001 ctaggaagca ctccataacc atcttgtagt tcttgggcat gcggaacaag ccgttcaata
    15061 aattgctgat gggattgcgc ttcattttct tgctgattat cagtaaaagg agaagacaga
    15121 ttcttcaagg aaaaaacaaa ggctaagagt atcactgcta caccggcaac cactgctggc
    15181 caacggatat tcttgcgctt ttgctttttc cacttttttt tcgccatgat caattcctac
    15241 ttttcattta attcattcag atattcttct agcgtcaatt ggtgtttggt gatatactca
    15301 gcgttttctt taccgacata acgtaagtgc caaggttcat atgtgatctt agttatatct
    15361 tcgtgtcctt ttggataacg aacgataaat ccatactgcg cagcattatc tgcgatccat
    15421 ttagctccag gctgatctcc gtaacttgca tccaatacag tagaaggata actgttgtac
    15481 cagttttgat cgacgacatc gactgccaga cctgtgtggt gttcgctgta tcctggctcc
    15541 gtaatcgttt ctttagtaat ctttgtcgcc tcctcttctg atacaccttg acgactcatc
    15601 acatcttgga cattttgttc gaaaacttgt tgttgggatg atacagaccg gtaagctgag
    15661 accatcacta atggaaatcc agctgcttca gcagccttgg caaatgcttc atattctgga
    15721 gcaatgcgtt tgtcgatcaa gtatccgtta cttaatgatg ccaattgagt agactcatca
    15781 atttcttttt ctaatttgtg atctggacca accaaaacta gcttccaatc gtctgtttta
    15841 acatctggca attgtttgga cgtacgatct tttgtttctt tgtcctttac cttgctgctg
    15901 gttgtttgac tgttcttttc tttactagca gttgttttgg tgcttgttcg ttcgactgct
    15961 tgattgccgg cttgaacatg cgaatcatca tcgctaaaaa taacatagcc cattgctgcg
    16021 atcataatca caactgctcc aaatcctaaa aatttattca cttgaaaaat cctttcgttg
    16081 ttcttcacgt tccccattcg cctcatgaac gtaagtttct tccagatttt cattcaccca
    16141 atcgagaagt tcgatttttt ctccttcgat ctgtttttga acatctgctg gcagacgaaa
    16201 attcaaaata tcatcatagc cccattcatc atattgaata cttactttca agtcaaggta
    16261 tctaccagcc tgctgattat ttttcaaagg gatcagctcg acttttgctg ctttgctgct
    16321 aagccatttt tgcgcgatgc gtgttttgat ttgcttgtac tctgcaacgg caacgtttcg
    16381 tttttcggga aaaataatcg tttgacgcaa aatcttttgc gttagcatcc attcataatc
    16441 agtgaaaaga ttttttactt tctgcatcgc ttcagatggc agatttttct cgccatcttt
    16501 ccatttttgc cattgttctg gactgatcag caagtattca tctaaaaaat ttttttcagt
    16561 ttcaaactgt tctaataata cactcataat aagatcaata tatacttgat gcacgtttat
    16621 cccctccttt taactattct acttaagttt tgatcaaaaa gcacctctta aatcgctgct
    16681 ttcatttttt ttaatgtttc aagtcctctc ctctttattc ttcgtgattt ttgttataat
    16741 aatagacaga caagaaactc aatccaacta tgaaggagcg ttttcagatg aaaaaatatc
    16801 aatggggaat cgttggtctt ggtaccatcg cacatgagtt tgccgaaagc ttcaatcaag
    16861 agacgagcga actaacagca gttgcttcca gaacttcaga aaaagcggaa aacttcgctc
    16921 accgttacaa tattccaaaa gcttacggaa gctatcaaga aatgttagac gatgctgaaa
    16981 tcgacatcgt gtacattgct gtaccaaata gacagcatat cgaccatatc ttggcagcat
    17041 tggaagcagg caaacacgtt ctatgtgaaa aagccatcac gatgaataaa aaagaactgg
    17101 ctgacgctat gcgcttagca gaagaaaaga atttgatctt agcagaagcg atgacaatct
    17161 tcaacatgcc actttatcag cagttacgct caataatgga tacaggaaaa ctgggggctt
    17221 taaaaatgat ccaagcacct ttcggcagtt acaaagaacc agaccctaaa aaccggttct
    17281 tcaatccaga attagctgga ggagctctgt tggatatcgg tacgtatgct gtatctttcg
    17341 ctcgtttctt tttgagctct caacctgaag tagttgcctc tacaatggtt ccttttgaaa
    17401 caggcgtgga cgaacaatcc gtcactatct tgcgtaacaa agaaaatgaa cttgctgctg
    17461 tcagcttgac ttttcaggca aaaatgccaa aagtcggtat cgttgccttt gagaacggtt
    17521 atgtgacgat tgctgattac ccgcgtgctg atcgtgcgga gatcttattt acagatggta
    17581 caaaggaatt catcgaaagc gggaatacat ctgaagcaat gaattacgaa ataaagaata
    17641 tggtacacat gattgaaggt gacttgccca atcgctctct cttcttgaca aaagatgtca
    17701 tcgaaatctt ggatcaaatg caagttctat ggaaaaatat gtaattttaa aaggttgtga
    17761 aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagctcc tcctattttt
    17821 cgtttggctg gacttattac cgcagaggaa agcttttgaa caccgtttaa gaaagaggtt
    17881 gtgagcctac cgttcagctc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt
    17941 ttgttgaata ttgtgacttg tgaccgagga gttgacagga gaacaccgtt taaagaagag
    18001 gttgtgaaat cagctttttg acctgaatat tagctgaatt cacaacctct atttttggat
    18061 tttttgttgg gagaattctg ccttgatttc aataaaactt ttaaaagact gcaaaaactg
    18121 gaacaattct gctcgatcct cgaattcagg gcgactagtc ggcagaggca tttgccgata
    18181 caatacataa acttcttcaa tacgtgttaa aatttgtctg ccatcatttg actctgaaaa
    18241 tgtttctgca gtataaatca ataaaccata gatatgtttt ccataataag ctggttgctg
    18301 gattctctct agattttcca gcatgtcttt aatcacattg gcctgtgctc ttctcataga
    18361 gaagtaagtt tcgtagtata gcggctgtct gagccagtag ttttcttgat gttctctggc
    18421 atcttcttgg ctttcgcgaa tgaatgtcag taaatgttcg catttgatct gcacatcttc
    18481 tttattttct gataaaagaa attctgccat ttgtctcaaa atatttcgaa attctttttc
    18541 gatctgtatt tggttttcct tcagctgctt cgtacgatct ggcatgtaaa catttgcaag
    18601 caaagcaaat actaatccaa tcgccattaa tcccgcttca ttgccgatca gctgccaaga
    18661 catgttttcc tccactaaat aatgagtgac caaaaccgaa tttactacta tcccttcggt
    18721 taatttgaag cgagaggcaa gcgggatgaa caggaataaa aaaagactga agccaaaaat
    18781 agtataccca acagcaaaaa aactgaacag tgctgttgcc gtagctaata taaaggctgc
    18841 gattcgattc atgcccgtac gcaatgtaga gcgcttggta tttcctacac ttaataatgc
    18901 aatgatccct gcagctggcc aatacaacag cgataagcta cccgctgtga ccatcgaaaa
    18961 gaagacactg atcactgttt tgactgttcg cattcctata cgcatttact tccccctttt
    19021 ttattttaat tatacttgaa taatgaaaaa ctagctatca gacaaaagtt taggtatgat
    19081 agtaaaaaga ccaaaaagga gaatctctat gcaatttggc cgttttcgac taggtatgcg
    19141 tacgatcaaa agtgccttag ctgttttttt atgtatcttg ttttttcatt tgactcaacg
    19201 aggaatcccg atgattgctg cattatctgc tgttttttct ttgcgtcagg acttgactac
    19261 aaccgtttct tttggaaagt ctaggatcat cggcaataca ttaggtggta cattagctat
    19321 catttatttt tttgtcaaaa gctattttca caatgatttt ttggtagaat tgatcttgct
    19381 tcctattcta gttattattg tgatcgtcgt gtcagatggt attaacaata attccggtat
    19441 catttccgca atagctacgt tgttgctgat tgcgttgagc atccctcaag gcgaatccgc
    19501 tttgtatgct atccaacgtg tactagatac ttttataggg acattcattg caatcggtat
    19561 caatttcttt cttcgtcctc ctgaaacaga aaagaaggaa gaactatcag aagatttggt
    19621 cgaactccaa aagaaagaac gattgctaag aacaaattta caagagatag aagctaagat
    19681 ccaaaaacaa aagaaaaata gcgaggacta attttctttt gcaataaaat aaaaaaatgg
    19741 agagaagaac tttaaaaagt cttcctccat ttttgattga acggtgaagt gtactacttt
    19801 attcttcctt catcgagttt tgttctgcct cttttggcgc ttcatacata tttacttggc
    19861 tagtagtccc acgtgtcgac aaattctcta cgatttcctt caagtcaagt cctgttgtcg
    19921 cttttagtgt ttcttgagta gaagaaagca aatttgtggc ataatttgtc actcggttcg
    19981 ctccactggt ttctcctgaa ctgcttcctg tatcgacaac agagattttt tcgatatttc
    20041 ctagcggctg tgctgcttcg cgcatcaatt gcggtagcat atcgataacc atactcaaga
    20101 cagctgcttc gccatattcc ttgaaggcat tggcgatctt ttgttttgct tcggcttctg
    20161 cttttccttt tgctagtgct gcttcggctt ctgcttgtcc agctaaacgt gtcttattgg
    20221 cttccgcttc tgccagtgct tccacacgga atcgttctgc ttcggcttca gtcacttctt
    20281 tgactttttg agcttgtgct tcttgttctt tagcgtaccg atcggcatct gcttttttct
    20341 tcacttccga atcatattgc ttttcacgac gtgtgatttc tttttcttcc agttcgattt
    20401 gtttttgacg ttctacgatt ttaatctgca tttcctgttc gattacttgt tgttgtgctc
    20461 gggcactttc taaattgtag gcttggtctg cttttgcttt agcaacatct tgttcttgtt
    20521 tgtaggttgc taatttcagt tctttttctt ttaatgattc tgcgatctct gtttgtcgct
    20581 gtaattcagc tgcttgtgat tctttttctg cttgagcttt tttgatacgc gtttctttta
    20641 gagcttccgc ttctgctata tctgcatctc ttttgacttg ggcgattctt ggtttcccta
    20701 aagagtcaag ataaccgttt ttgtctctga cctctttgat agtgaaagaa acgatgatca
    20761 accccatttt tgcaaggtct acagaagcga cttcttgtac gctttgactg aacttatcgc
    20821 gattttgata gatttcttct acagtcatag aaccaagaat cgagcgcaag tgtccttcta
    20881 atacttctct tgcttcattt tccagttctt ctcttgtctt gcctaaaaac tgttctgctg
    20941 ctgtagcaat ctcttctaca gatgaaccga ttttgatgat cgaagtacca tcacacatca
    21001 ccggtacgcc ttgttccgta tacacttcag gggtggagac gtccaattta ctcgataata
    21061 gactgatccg attcgaacgt tgaaaaactg gcaagacgaa tgcgccacct ccacgcacaa
    21121 ttttgatttt gttattgctt tcatcaatgt gcacattttt gcttcctaaa taacttccac
    21181 tgatgatcaa cgcttcatcc ggttttgctg tctggtactt cgctacaaag acgatcagca
    21241 acatcagtaa aatcaaagct gcgattgcga tcgcgataaa aacaactggt atttccattt
    21301 aacacacgct cctttaagct tttatcatgc tttcataagg catgacataa cagattcttt
    21361 ccttgatctc aatgatcaat actttcgttc cccgctcagc tgtctgttct tgggggctgt
    21421 aaaaacaagc tggacgacta atggaaccgg taacactttt caactgaatt tctcccatac
    21481 ctcgtacagg gatcggtgtg atcacaacag ctacttgtcc ttccagagtt ttttcagaag
    21541 acgacatcgt tgattcggca tgtttcatcg gcatcaatat atagaaatta agcaaaaata
    21601 ccaacactat cgatatgcct ccactagcaa taaataaaaa taaagatgat tgatctgtta
    21661 aacgctcacc tagaaaacca aataagcttg tgaaagttaa ccaagggata ataagcatcg
    21721 gatcaagcgg gccatcaaaa tcgaatacat ctccaaaaat aatcaatagg actgccaatc
    21781 ctgcacaaat cagcaatgta taccaataaa tcgtctcaag tgcgattccc cctatcatat
    21841 acacgcctcc ttccatttct acgtctatca taccaatgtt tgaaaattct gtatagcttc
    21901 ttttaccgac tcagtggtgt tttttatagg aaggttgtgt ttatcgttga caatttgtta
    21961 aatttttgtt ttcatgttaa aattttcaca ataaattctt tcgaggtagc ctatgtttcc
    22021 tttttttaag aaaaaaccat taaagctcca gaacacgatc atccttgtcg tttgtttctc
    22081 cacattcgtt tctttgattg tttctgccat attgatcagg aattttattc tttcgatgaa
    22141 ccgatctagt gtgtcagata aaatctctag cattgctaaa atcgttgcca atgataaact
    22201 catccaaaaa aagctgcaga ctggtgaccc gacacatgcg attcaaggaa taacaaatga
    22261 aatcatgatt gcatcgaatt tagattttat cgttgtcatg gaccctgaag gcattcgata
    22321 ttctcacgca gacccaagtt acatcggtct tcctttctca agtcatgaag atgtgaaaaa
    22381 agtgattgga ggacaagaat ttttctctga aaaagaagga atgttagggt tcggcttccg
    22441 ctatttcgtc cctatttatt atgatcaaga gattattggt gcagtttgtg ttggcttaac
    22501 aaacaacacg attgacaatc aagtcaaaga tggacaaaaa atgattgttt tttcattgat
    22561 tattggactt agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt
    22621 actgcttgga cttgaaccac aagaaatagc agaacagtta agtgaaaaaa cgattatcga
    22681 aaatgaagta gatgaaggaa tcttagccat ctcttctgat aaaactatcc tcttgatcaa
    22741 tacacctgca aaaaacattt tctaccaaac agagcagcta ttggatttcg aagtaggaaa
    22801 aaaatcagtg ataatctata tgacgttttg tttaaagaga cgattgagca tcaaacgacc
    22861 gttcggactc aatccatgta tttaaatggg atagaaacta tttctagcgt tacacctctt
    22921 tttagcaatg accgtttttt aggagcagtc gcaactttac gaaatcgatc agaagtaatg
    22981 aaattaatgg aggagcttgg ggggacgaaa caatatataa actccatgag agctcaaacc
    23041 catgaattca tgaataaact tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa
    23101 gaagtcaccc attttatcca gcagctaaat caaaattata aaaaagagat tggccaagtc
    23161 tcaagtctga tcaaagtacc tgcaattgcc ggttttttat taggcaaaat aaacgaagcc
    23221 aaagaacaat ctgttgaaat gctgttagaa agcgattctt ttatccctaa aatggagatg
    23281 gatgactcca tccattcact tttgcaagtg ttagggaact tacttgataa tgcaaaagaa
    23341 gccgtgctac aaaaacatga aagaaacgga caggtccgga ttcatttaaa ctatgacaca
    23401 gaagctgaag tattcatcgt gaccgtctgc gatgacggtg tcgggatatc agaagaaatc
    23461 tcagaaaaaa tatttacgct aggtttttcg acgaagggaa tcaatcgtgg gtttggattg
    23521 aatctcatcc attcaatcgt caaacgccac ggtggatttt tagaattttc caaacaaaca
    23581 aatggcggta caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa
    23641 tgtcctgata atcgaagatg atccaatggt tgcagaattg aatcgtactt ttgtggaaag
    23701 catccctaat atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt
    23761 cagacaaaaa aacgcagatc tgctgctgct tgatgtatac atgcctggcg aaagtggtat
    23821 ccatttttta tcttggctta gacagcaagg aaatgatatc ccagtgattt tgatcactgc
    23881 tgcagatgat atcgaaacca taaagaatgt gatccgctac ggtgccttag attatttgat
    23941 caaaccgttc actttgcaac gttttcagct ggcgatcgaa aagtatcttg ctttctctca
    24001 gacaatccaa acgactgaaa aagccgatca gaaattgatc gatcaagtat tcaaccacac
    24061 cactcccatt cacgaaatac catcattcat cgatttgcct aaaggtctgt caaaactgac
    24121 tttaaaaaaa ataagtgcat ttattctaaa agaagatact attttttcaa caaaagaact
    24181 ggctcaaaaa agctcactgt cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga
    24241 acaagatata ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc taacctgtta
    24301 tgaattgaca aagaatgggc ggaaaaagct cacagattac tgttgagtca tcgaattatc
    24361 ccatacaaaa tagtccaagt atacagaagt aacaactgtt tacttggact atttttaaac
    24421 attttattga cgaatatcca tgttttctta tcttaattga cgggctttag gaagagatac
    24481 ctcatttttc gtttttccag tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat
    24541 catatttgat aatgcctcat ctgtatttga acctacatgc ggggttacaa gaactctagg
    24601 gtacatttct actaattgct ggatagcagg gtctggaatc gtctcccatg gcttgaagga
    24661 tcggaaaaaa acctctgttt catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt
    24721 gcttttcaaa gctcttaaaa tagcttcatt atcttgaagc tctccacggg ctgtattaat
    24781 caagatgcta ccttttttca tttgagctaa aaatccttca ttgatcattt gatcgttttc
    24841 acctgggata tgaggtacat gaatactgac aatatcgctt ttggctaata actcttcaag
    24901 cgagtggaac gtcaatactt ttttagcagc ttctgtctga tgaatatcgt agccgatcac
    24961 tttggctcct agtcccttaa acaaaacagc ttccgtcaac cctatcctgc ctgttcctat
    25021 gatccctact gtgcaatttc tgatttcacg actaaacatt tgctcatcca ctcgaaaatc
    25081 tttgaaagat gttcggatcg tcgtatacgc agtatttctc aaaagcatca tggcaaaagt
    25141 caaagataac tctgctatcg catttggcga atacgatggt acacgggcta ctgtcatacc
    25201 aaaatcagct gccgcttgta gatcgatatg attgactccc acagtccgtg taaaaacata
    25261 acgaatccca tattcctgca tttttgcaag attggtctca tctgctatac aatttcccct
    25321 caacaataca gcatcatggt cctttgctga cacgatattg tcatgtgtca acagttcttc
    25381 ataaagactg agatcataat ggtaaatatt caatttttca aaaaaatcta tctcgttcgg
    25441 acgaacaccg taacatgcga ttctcatact ttcttcctcc atttcttatg aaaataaacc
    25501 tatgccatga atcatttgta atacaacgat ccatacaggt gtcagtacca cagcaaaaat
    25561 agttgacagt aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac tgatagcata
    25621 ggctgctgcc actgttgcag ctggtgtagc catcatgata acgattgttg ccagtgcttc
    25681 aaaattgact ggtagaatat tcgttgccgt caaaactgct agaatcacga tattcaatgc
    25741 aggtacaaat accactttga ctagactgta ataccatgaa gttttttcag atgcagcttt
    25801 tttgaagctt acttcaccta atgttgcgcc gattgctagc cacgccaatg gtgaacttaa
    25861 accagctaaa taatccatcg actgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa
    25921 cgctacttgg cttatctctc cttctgcacc tgtaactgac acttgtggca gatacccttg
    25981 gaacagccaa atgaaaaggc cggcaaaagt agcgataatg attggattga cgaacatcgc
    26041 tttgatattt ttagcttcca tcttcaagcc actcattttt atgtatccat aagaatacaa
    26101 aaagattcgg tagccaatat tgaaaattga ggaatacatc acgccgacag gaccataaat
    26161 cgcactgacg ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa
    26221 cgttgtttgc ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta gaataagaat
    26281 aatataaata actaatcccc aaatcaagac attgattcct tgcatcagta attctgtatt
    26341 gatatcttgc atgaaagctc gaaatgccag tgctggcaaa gccactgtta agacgacttt
    26401 cgatagaatt tttccaacag attcagaaaa aatccccttt tttctacaaa caaaccctaa
    26461 taatataata aaaactgttg aagtgattgc actgacaatc gacatatct
//
LOCUS       DASJTM010000036        26281 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJTM010000036 DASJTM010000000
VERSION     DASJTM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.036"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1416
                     /locus_tag="U4B29_001392"
                     /pseudo
     CDS             1..1416
                     /locus_tag="U4B29_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1524..2606)
                     /gene="uxuA"
                     /locus_tag="U4B29_001393"
     CDS             complement(1524..2606)
                     /gene="uxuA"
                     /locus_tag="U4B29_001393"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2106349.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2597..4243)
                     /locus_tag="U4B29_001394"
     CDS             complement(2597..4243)
                     /locus_tag="U4B29_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2106350.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4427..5089
                     /locus_tag="U4B29_001395"
     CDS             4427..5089
                     /locus_tag="U4B29_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2106351.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5233..5796
                     /gene="ahpC"
                     /locus_tag="U4B29_001396"
     CDS             5233..5796
                     /gene="ahpC"
                     /locus_tag="U4B29_001396"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN2106352.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            5806..7335
                     /gene="ahpF"
                     /locus_tag="U4B29_001397"
     CDS             5806..7335
                     /gene="ahpF"
                     /locus_tag="U4B29_001397"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN2106353.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            7492..7842
                     /locus_tag="U4B29_001398"
     CDS             7492..7842
                     /locus_tag="U4B29_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN2106354.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            7941..8471
                     /locus_tag="U4B29_001399"
     CDS             7941..8471
                     /locus_tag="U4B29_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2106355.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            8625..9776
                     /locus_tag="U4B29_001400"
     CDS             8625..9776
                     /locus_tag="U4B29_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2106356.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9885..11924)
                     /locus_tag="U4B29_001401"
     CDS             complement(9885..11924)
                     /locus_tag="U4B29_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN2106357.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(11982..12539)
                     /locus_tag="U4B29_001402"
                     /pseudo
     CDS             complement(11982..12539)
                     /locus_tag="U4B29_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(13078..13284)
                     /locus_tag="U4B29_001403"
     CDS             complement(13078..13284)
                     /locus_tag="U4B29_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106358.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(13300..13914)
                     /locus_tag="U4B29_001404"
     CDS             complement(13300..13914)
                     /locus_tag="U4B29_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN2106359.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(14061..14744)
                     /locus_tag="U4B29_001405"
     CDS             complement(14061..14744)
                     /locus_tag="U4B29_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2106360.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(14853..15311)
                     /locus_tag="U4B29_001406"
     CDS             complement(14853..15311)
                     /locus_tag="U4B29_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN2106361.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(15395..17158)
                     /locus_tag="U4B29_001407"
     CDS             complement(15395..17158)
                     /locus_tag="U4B29_001407"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN2106362.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(17379..18839)
                     /locus_tag="U4B29_001408"
     CDS             complement(17379..18839)
                     /locus_tag="U4B29_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106363.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(19047..19973)
                     /gene="glsA"
                     /locus_tag="U4B29_001409"
     CDS             complement(19047..19973)
                     /gene="glsA"
                     /locus_tag="U4B29_001409"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN2106364.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(19996..21423)
                     /locus_tag="U4B29_001410"
     CDS             complement(19996..21423)
                     /locus_tag="U4B29_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106365.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(21454..22668)
                     /locus_tag="U4B29_001411"
     CDS             complement(21454..22668)
                     /locus_tag="U4B29_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN2106366.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            23018..24097
                     /locus_tag="U4B29_001412"
     CDS             23018..24097
                     /locus_tag="U4B29_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2106367.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(24398..24640)
                     /locus_tag="U4B29_001413"
     CDS             complement(24398..24640)
                     /locus_tag="U4B29_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2106368.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(24672..25229)
                     /locus_tag="U4B29_001414"
     CDS             complement(24672..25229)
                     /locus_tag="U4B29_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2106369.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(25242..25430)
                     /locus_tag="U4B29_001415"
     CDS             complement(25242..25430)
                     /locus_tag="U4B29_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2106370.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(25443..26009)
                     /gene="amaP"
                     /locus_tag="U4B29_001416"
     CDS             complement(25443..26009)
                     /gene="amaP"
                     /locus_tag="U4B29_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2106371.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         7721 a         5232 c         4707 g         8621 t
ORIGIN      
        1 tcaaattttt tactatacat caaagaatta gaacaagact ttgagcgtct caatctaaca
       61 gaactgcata ttgataagca aaaacctttt ttaaagttga attttgaagg aattgatccg
      121 gcttactgct attatcgtct atttggaaga tactgcaacg aatcagtcag ttatcagatc
      181 ttgacttctt tgttcagctg tcagacaaat tcgattatca gtttcagtca gcaaacaaac
      241 tatagtgcct cttatttgta tacgaaaatg aaaaagatta atgcattttt agcatagtac
      301 ggtttgtcta tctccttttc taataatggc agaaagtctt tttctggaaa agaggtacaa
      361 attcaatatg cagcattaga tatttattgg aacatctttt caagtactgc tacagaattt
      421 tatgatgaag atccgcaagt agtggctttt atgatgaata ctttttttaa aaaagaagta
      481 cttgaccgac taaatagcgg tatgatagat aaattatatt ttctattaaa aatatgtaaa
      541 gagaattttc ctgcaacttc tgtgaaagac gtacaaaaag aattcacaga gtatgaatat
      601 attgactttt ttattgatct ctctgtagat attttgcgac ctaatctgcc aatttgtcgc
      661 gaacaacgca tcttagtgaa tattttagct cgactttcta tcacgaaaat cgaatcggaa
      721 gaaacaagct tggagcaata caaactttta aaaaaagcaa acgttcctca tgttctttat
      781 tcaagagaaa tggtggagtc cctttgtact gtatttgatt tgtttatacc tgaaaaagaa
      841 aagattcttt actcattggc attcgcacga aataaacttt ataatggatt tcttaacttc
      901 aatcaaccaa atacgcccct tcctactttc atgctttatc aagaacatag cgtattgaaa
      961 gaagtccaaa ccgccattga acaattttat atggatttca gagaacaaaa tcaacacttg
     1021 ttaccagtta ttttagaaaa ggaaaatgtg caatggatgg ttgaagacct agtacatttg
     1081 tatgatcgct acaaaaaaca gccgcgaatt gtgattggtg tgaactatac gcgtgatttt
     1141 tatatcagta aagatctgat cgttaagata gaacaaattt tttcaaatga atcgatcaaa
     1201 atacaaaatg attttatgga gaactgcaac atcgttatca gcgactgccc tttgaaacaa
     1261 ttgccttctc atatcaaaaa aatctatatt ttaaaaggaa tcatcacacc agaagactgg
     1321 aaaaaagtga ttatgaggat ctcagaatat atctttgaat taaaagaaga agcactcatg
     1381 gatcaatcga aaaaagaaaa tcaagttact tcctagacca aataagatac ataaatccga
     1441 atgcttatta acaacctccg gatttatgta tcaagaattt gaacatcctt cttatttaca
     1501 taataaaatt acagtttttt tcactaatta tctttacttt tgtttccttt ttcgattgct
     1561 tcccataatc catttagata aacagcacct aatgctcgat catataagcc gtatcctggt
     1621 cttcctgtct cattccagat cattcttcca tgatcaggac ggatgtatcc gttaaatccg
     1681 ttgtcataca ttgtttttag tatgcggtac atgtctaatg aaccgtattc ggatttatgt
     1741 ggtgcctcat agaaatctcg attagctaaa aatttaatat ttcgaacatg tgcaaatggg
     1801 attcgatctc tttggcaaaa ttctgctaaa atcccatata catcattttc tggatcttct
     1861 gcaatactcc ctgtacaaag agtgattccg ttataagggc tgtctactat gcgacacaac
     1921 caatctaaat cttctcgatt ctttacgatt cgagctaatc caaaaatcga gtatggcgga
     1981 tcatctggat gtacagccat tttgacccct acctcttcac atgttgggat gattgattgg
     2041 agaaaatagg caaaattttc tcgaagtttt tcttcgtcta ctgatgcata ggcttcaagc
     2101 aaggatttga taggagctaa gcgctcaggt tcccatcccg gcaatccaaa ttcattgctg
     2161 gaatgttcga tgcgcgcgac gatttctctt ggatcatctg gaatatcatc agatataaaa
     2221 gctaacgtgg atgagccgtc ttctaagcgg tagtcaaggt cagatttcac ccagtcaaat
     2281 acaggcataa aattatagca gatgacttct attccatatt ctgccaagtt tcggattgtc
     2341 tgcttatagt tttcgatata ctgctcacgg gaaggtaatc caattttaat ggcatcatgc
     2401 acattgacgc tttcaattac ttttagtgtt aacccacgag actcaatttc ttcttttagt
     2461 tctttgatcc tctccctagg ccatacctct cctactggta tatccatcaa tgtgcctaca
     2521 atccctttga ccgcaggaat ctgtctgatt tcatctaagg taatcttttc ttcctgtgat
     2581 ccgtaccatc taaaagtcat ttccatgata aatttcctcc atgttcttgt aagtaatcct
     2641 gtaatgtttt tcgtacggca tttggtcctg ctaccatctt gatgaacatt tcttctattt
     2701 ttctgccaag ctctgcttgg taaagatcta caccaaaaat ttctttattt gacaaaatag
     2761 gagccatttt ctcatgaata tgtgatgtat cttgcgtacc cagttgaata tcggaaagct
     2821 tatgctgaag gtctgtcaat agtgggtctg gactcgggat gaatggtttt ccttgatcat
     2881 caatgccaat aaggtatcga agccaagcag cgagagtcaa tggtatgaat acaagtgtct
     2941 gcgggtcttt aacttctatt tccatatatt ttttaatcgt ctcaccataa cgaatcgcca
     3001 ccttttgaga ggtatccgtg gcgattcgct gaggcatatt aggaatattt ggattagaaa
     3061 agcgcacagt aattacttct tccagaaaga tctttggatc aatgatacct ggagactcca
     3121 caactgggag gccttcattg tacccgattt cagaaactag cagcgataaa tctggatctt
     3181 tcatctcctc agctatgctc ctatacccca gtaaacaacc cataatagct aaagacgtat
     3241 gcaaaggatt aaggcaagtc gtcaccttca tcgtatctgc tttatctacc gttgcacgat
     3301 cagtcaagaa taccccagct ttttcgaatt tcggacgtcc attgggaaaa tgatcttcca
     3361 tgattaaata atggatgtcc tctgtattga caaatggagc aatattattt ccttttgcgg
     3421 tttggaaaat cgacatattt gaaaagccct cttttgccaa cattttgcta actgacaagg
     3481 aaggattcgg ggtgatacga tcaatcatcg tccatggaaa cgcaacaatt tcttcattcg
     3541 ataagtattg gataaatgta ttttgtacat aaccttcctt ttgccattct tttgcgatcg
     3601 ttagcaaaga agtacgtaat tgactaccgt tttgtgaaaa attatctgta gagacaagtg
     3661 caatcggcca agctccagat aaaaaacgtt tataaagtag cacagcaaga atactcattg
     3721 tatgtgatgg cttctttgga ccatttttta tatcgactgc aatcggtcct atatatttat
     3781 tatttgtatc tttcaatcga taaccttttt cagtaatagc aaaagtaact aattgtaaac
     3841 taggattttc aaagtgtcga atcatcttat caaagtgttc agaacaatct ggatgacaat
     3901 acagactgtc agctgtcgca gctaatatac gtttgacgaa ctctccactg cttggcatga
     3961 ccacttcaag aatcaaatca tcaaaatctt ggtaagccct ttcaattatt tcttcatcga
     4021 atgtttcaca aacaatgaca ccagtggaca attcgcctag atccgctaaa cgttgagcga
     4081 tttctgcatg aaatcctcga taaagatttc cgccaccgaa atggatccag acaggatttt
     4141 tttgtccatt ttctcttaat gatgcacggt caaaatgagg tatttctact tctttttgag
     4201 ataattcagg gataaagttc ctccagttgt ttaaatcaat catttttccc ctcctaaact
     4261 aacatattag tttttgtatt caagtgaatt ttatgcaata aaaatcgcta tgtcaaccaa
     4321 aattttacta tttatacaaa tttactagtt aatgtgacat accagattgt ttttcagacc
     4381 tcttctgtta tactacgttc atagaaatga ttttggagga ataaagatga aaagcagctt
     4441 gcaatcacaa gcttatcaag cgattcgtaa acaaattatc tatgctgact tagaacctgg
     4501 aaggaaaata tctgaaaagg cactggagga acatcttgct atcggccgga ctcccattcg
     4561 cgaagcattg attcaactac gtcagcaaga actcgtgtat acgatacctc aaagtggtac
     4621 gtatgtttcc aaaattaatc ttgtttctgc agaaaatgct cgctttgtcc gagaacagtt
     4681 ggaagagcaa atcatgattg aatgttgttc caaattggac acacaaacac gaatagtgct
     4741 agaaacgatt ttagaagaac aagaaaaagc agtcaaaata aatgacgaac gcgctttttt
     4801 ccgtgcagat aatttatttc atgaagcttg tttcgaaatt gcaggaagaa aagaagtttg
     4861 gaactggtta gatgaccata atacccattt agaacgtttc cgctggttac gagtgaccat
     4921 tcaggggctt ccatgggaaa cgatcatgga acaacattat gatttactca aggctttagt
     4981 tgataaaaaa ccagaagaag ctcgatttca aacgggattg cacttacaca tgatgctgaa
     5041 agaacaggaa actgttactc aacgttatcc cgagtatttc cagcagtagc attttcttgc
     5101 gacctcttct atttttataa ttaaccttcg tttttgagta atctttatta aataacaaaa
     5161 tatccattga ctttgcaact ccaactaaat tacaataatt ataaataaat aattattatt
     5221 ggaggtttta ctatgtcatt gattgggaaa aaaatcgaag aatttaatgc acatgcttat
     5281 catcaaggag aatttttaga agtcagtgaa aaagatctta tggggaactg gagtattctt
     5341 tgcttctatc cagctgattt tacttttgtt tgtcctactg aattagaaga cttacaagaa
     5401 caatacagta cattgaaatc tttaggtgtt gaagtctttt cttgctctac cgatacccac
     5461 tttactcaca aagcatggca tgatacatca gatgctatcg gtaaaatcga atacgtgatg
     5521 atcggtgatc cgtctcatca gatttctcgt atttttgatg ttttggatga agagcaagga
     5581 ttagctcagc gaggaacatt tattattgat ccagacggaa tcgttcaagc tatggaaatc
     5641 aatgcagatg gaatcggtcg tgacgctagt gcactgattg ataagattcg cgctgctcaa
     5701 tatgtacgta cccacccagg tgaagtttgt ccggcaaaat ggaaagaaag tggcgaaaca
     5761 ttgaaaccaa gttttgatct tgttggaaag atttaggtgg tggatatgtt agacaaagaa
     5821 actaaacaaa atctcgaaca atatttagca ctgattgaat ctcctattgt tttttctgtt
     5881 agtttggata atagtgaaaa tagtcaaaaa ctagctgaat ttacgaagga aatagcagaa
     5941 atgtctccta aaatctcttg ggaaaaaaga gattcgatgc gcacacctag tttttctatc
     6001 aatccagttg gcaaagaaag tggaatcgtt tttgctggta tccccctcgg tcatgaattt
     6061 tcctcttttt tactggctat gttacaaatt agtggacggg caccgaaaat cagtgaatct
     6121 ttgagtctta aaatcaaaaa aataacacaa acattgcatt ttgacagtta tgttagcttg
     6181 acttgtcata attgccccga cgttgtacag gcattgaata tattagctgt attaaatcca
     6241 aatattactc atacgatgat tgaaggtggg atgttccaaa aagaaatcga tgataaaaaa
     6301 atcatggcag tgccaactgt gtttttaaat ggagaagaat ttagtagcgg acgcatgacg
     6361 atcgaacaaa ttgttgaaaa aatcactggc cctttgattg aagaagagtt gtttgaaaaa
     6421 gaaccttatg atgtattagt gattggcggt ggaccagcag ctagtagtgc agctatctat
     6481 gcagcacgta aaggaatccg tacaggactt gtcgctgatt ctttcggcgg acaagtcgtt
     6541 gaaacactag ggattgaaaa tatgattggt acctcttaca cagaaggtcc aaaattgatg
     6601 cagcaagtag aaagtcatgt gcgtagttac cctatcgaca ttatgatgaa tcaacaggcc
     6661 gtttccttaa ataaagagca acattttatt aatattggct tagctaacgg tatatcactt
     6721 aaagcaaaaa ccgccatcat cgcgacaggt gcgcactggc gttctatcaa tgtaccaggc
     6781 gaaaaagagt ttaaaaataa aggaattgct tattgcccac actgtgacgg ccctttattt
     6841 aaaggaaaag agattgctgt tatcggtggt ggtaattctg gtgttgaagc agcaatcgat
     6901 ttggctgggt tagcaaaaca tgtatatgtt ctagaattct taccagaatt aaaagcagat
     6961 caaatcttac aggataaact agtctctctt gcgaatgtca ctgttatcaa acatgcagcg
     7021 acgaaagaaa tcagcggtac gaatcaggta gaatctctct cctatatcga tcgccgtacg
     7081 atggagatgc atacactagc tgtttcagga gtattcattc tagtcggctt actccctaat
     7141 acggattggc tagatagaac gatcgaaatg aatccaagaa atgaaatagt gaccgagaaa
     7201 aatggcgcaa cgaatatccc cggcgttttc gcagctggtg attgtacaga tagtccatac
     7261 aaacaaatca ttgtttcaat gggttcagga gctactgcag ctttaggagc attcgattat
     7321 ctgatgcgca cttgagctat gaagattgta aaaagtcaga gagcggaaaa aaggatggtg
     7381 acaagaattt tgtcacaatc ctttttgttt ttttgaataa tcgctttttc ttctcttttt
     7441 cttatatact ataagtagta aataatttga aaaaaagaaa ggtgactgat tatggcgcaa
     7501 aatcgtattt ttacgacaag ttttgcaagc gtttatccta tgtatgtagc aaaagcggag
     7561 agaaaagatc gtataaaaga ggaagtagat gaaatcattt gttggctgac gggttattcc
     7621 caaacagaac tagaatatca actacaaaaa caagtgactt tcgaacaatt ttttgcggaa
     7681 gcccctgtcc ccaatccttc acgttccttg attacaggtg ttgtttgtgg tgtacgagta
     7741 gaagacatcg aggataactt gatgaaagaa attcgttatt tagataaaat gatcgatgag
     7801 ctagcaaaag gcaaaaaaat ggaaaagatc ctcagaagtt gatttttcca gagatattct
     7861 tctccttcgc tcattttgta acatttaatt tggtaaaatg actagttcac gttaaaataa
     7921 gtcaaaggag gttttcttat atgtttgaga cgattaaaca aaatattcaa aaatacgcct
     7981 tattaagagc tgctgtttat attatttctg ggattgcgat tgtgctaaac ccttctgctg
     8041 tttttcattt tatcggttat ctgatcaccg cctatcttgt tttgcttggg ttgttgaatc
     8101 tttttgaggc ttaccgtttt aaaaggaaaa caggttcttg gagtatcggt ctgttcagtg
     8161 gtcttttttt cctgatctta gctctgatcg tctatgcttt tgcacctgca atcgtttcta
     8221 tcctgccgat tcttcttgga ttagctgtta tcatcaacag cttgttccag ttgttcttca
     8281 gcatgaatac aaaagctaaa agttggagtg tttatagtag tattctgttg ttgattggcg
     8341 gattcgtctt attgttcaat ccattcaaaa gtttgatgct tctattccaa atattcggct
     8401 ttattttgat tttcatgggc atccaggaga ttatcaattt catcagaata agaaaaatga
     8461 ctatacgtta atcattttta gttattaaaa tagaccgtat aaaaaccaac aatccaaaaa
     8521 aatcttttgg attgttggtt tttttgtgag tatattttcc tattttttca ataatgtatg
     8581 ttatgctaag ttcgggctat attttataca aaggaatgat gagaatggaa tttattggta
     8641 ttttatgcct gatattgatt tcaacaacga ttggctcaca tatctcgcgg agattaggta
     8701 tcccagcagt gattggacaa ttacttgttg gtgttttgct aggccaagct ggattgaatt
     8761 gggtcaaacc tgatattcta gtccatgact tttcggagat tggtgtgatc ttattaatgt
     8821 ttttagctgg tctggaaagt gatttatctc ttttgaaaaa atatttccga cctggtatgt
     8881 tcgttgcttt gcttgggatc atcttcccgg ttgtttttgg ctggttgaca ggcaaagcat
     8941 tccaagtttc gggaaatgaa gcgattttct ttgggattat tcttgcggca acttctgtca
     9001 gtatttcagt agaagtatta aaagaactaa acgtagtgaa tacaaaagaa ggatcaacaa
     9061 ttttgggtgc ttctgtggta gatgacattt tagtcgtttt agttctaagt ttcagcctat
     9121 cttttctaac gggggaagct tctagcaatg cttctttacc gcttgttttg attgaacagc
     9181 taatttattt tgtttttatt ttcttattag tcaaatggat tgcgcctttc ctactttcat
     9241 ttgcagaaaa agtgtacgca aattcttcta ttatcatcgt atcgctagtt atttgtttag
     9301 gaatgtctta cgtagcggat cttgttgggc ttagctctgt cattggtgct ttttttgctg
     9361 ggatagctgt cagtcagaca aaggttaaag aagaagtata ccacaatgta gaggctttag
     9421 gatatgcagt atttatacca gtattttttg tcagtgtagg attagaagtt gatttttctg
     9481 actttagtga acaactactc tttatttcta tattgacgct tgtagcgatt ttgaccaaat
     9541 tgcttggagg ttattttggt gctcagattg ccggtttttc tcaacatagt tcattgatgg
     9601 tcggtgctgg tatgatttcg cgtggagaaa tggctttgat cattttacaa attggacagc
     9661 aaagtcaatt gatcgaacca cagtactatt cacctttagt tatcgtgatt ttattaagta
     9721 cacttatttc tccacttgtt ctcaaatatt ttacaaaaaa agtttatgaa gtgtgagtaa
     9781 atcaaaaaga agttgtgaaa gagacatcgt gaaaaccgtt tatttgaaaa aaggactgtg
     9841 acgataacgc cacagtcctt ttactgtact taaaaagcaa ttttttattg ataattttgg
     9901 ttgaggtatt gcaatagttc cgatgctcgt ttggttgctg aatcatcatc ttctttattt
     9961 tccaacatgc tgaccatcat ataaccttgg ttatcaagat tgaaagcaaa caagaaactg
    10021 ttctcttgcc cctttacatc ctgtttttct ttgatttccg ctgtaccagt tttcgctgcc
    10081 aacggaatcc ctaaagcaga aagagaatgt gctgtaccat ttacatcttg cacaacttct
    10141 ctcagatctg gcacgatcgt ttgtactgct gtttcgctga tgacattctt cttatctttt
    10201 gtctctttat ctgcaatcaa tttaggatag acaagtgtgc cattgttgat aaaaacagaa
    10261 tacattgctg cttgctggat aggagtaatt agaagttcgc cctgtccata tccagtatca
    10321 gctagcaaga tatctgagtt aaagctatct tcattagaaa tttgtgctgg attcatacta
    10381 attggcaaat caaggtcttc accaaaaatg aatttatcta agcctgtacg aaattttttc
    10441 tcacccattt tcaacgtttc ttgtgccgca tatatattat cggaatagat caaagcagtt
    10501 tttaagtcta cttgtgatac atcactactg acacgcgttg ttttaaaaga tccccaagaa
    10561 ctgtcttttt gccatttaag cccgttgatc gtcaacactt catttggatc gatagtgccg
    10621 ttgtcgagac cgattgctgc tgtaatcatt ttaaacgtag atccaggagc ataacctgtc
    10681 gcaaatcggc tgatgaatgg atgttcagga ttttcttcat aagctttgta atcttcttgt
    10741 gagatcccgt ttgtcatttt gtttggatca tagcttggag agctagcaag cgcaagaaga
    10801 tcaccggttt ttggcgttgt cgcaacagtt gatgcagctt ttcctcctag actgtcaaaa
    10861 gctatttttt gtgccttcgc atcgattgtc aatttgatat ctttgccatt ttgaacttca
    10921 tgttcgatca gaactttttt ctctacaccg tctgcatctg taatgcttaa tttcccacct
    10981 gttgtcccac gaagatcctt atcaaaagcc atctccaaac ctgagcgacc gatttttcca
    11041 ttactgctta attctggatt cttatcaata tcttctgctg tgatatctcc cacgtaacca
    11101 atcagctgag cagctgcttc acccaaagga taatatctgc cgtctacttc ttgaatggta
    11161 gctccagctg gaagttctgg cgttgctcca tcaatgattt tcaatgggac aaagtaatcg
    11221 ggttgtaccc agctttgtga aatcgcctga ttgatagcat cttctgttaa gtcgaaggag
    11281 gaagcaatcg ctttgatatt ggctgttttt tcgcctccat ctccaagttt acttggaacg
    11341 actcctaatt gttttagttt gcctgttgtt gctaatggtt caccatttcg atctataata
    11401 ttccctcttg ctgcttcttg tgtggtcagg cttactttgt catttccttc catttctgga
    11461 aaaaccaagt tgggttgcca gttgatcgtg gtttgaccat catttctgtc taatgttcct
    11521 ttataagaaa gatctttcaa ttcacctaat gaggtattca tctttgcttt atatgaaaaa
    11581 ctgtaagtag aatcgtcttt tttatctact tttagattcg atatctgaag tcctttgaca
    11641 tcagcagcac cgtatatatt ctggtattta tctaagatct ctttttcaga taatgcactt
    11701 ttatttgccg ccttttttga tgtcatttct gcagctttgt tataatctcc cttgttcaaa
    11761 gcttggacaa attgctcaac tgtcttttct ccagcttcta ctgcttgggt ttcttgatag
    11821 tgctggtagt aaaaataacc gccacccgca gctgctatta agatcactgc gccggcaata
    11881 taagcgcctg ttcgattttt gccgtgcttg tcacttcttt tcattttttc ctccttctct
    11941 tgcttgtcat tcctttattt cactatttat acctgtttat tctaacatat gtctttgagg
    12001 aaatccatac cacaatccag aagcacactt tctggaaggt tcgtttggat actaccaagt
    12061 aatttcccaa ccgctttggg tagcttgaat attgaagtta cgtctttcag ctgctgagag
    12121 atagctttga tcacttgctg ctgccttctg atcctcccga agtctccttc tttgtcttcc
    12181 cgaaatcgag catactggag taaagtatta ccatccatca cttgctctcc tttgtttata
    12241 tatacgccat ctaaatctag agatttttct gcatcgatct tgactccacc aggaaacagt
    12301 tcattaacgc aatcgataaa atcttgaaat gtgatacttg cataataggg agcttcaaat
    12361 ttgaaatttt cctttaatgt ttgatttaat aaatcaactc ccccataaga gtaagctgca
    12421 tttatcttat tcattccgta acctggaata tcgacatatg aatctctcat gatagagatc
    12481 agttttgctt gttgtgtttt ctggttgtat tgtgcaacca gaagtgaatc cgagcgacct
    12541 ccaccaatcg atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg
    12601 aggagcgagt ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa
    12661 ccttttcgaa caagaattct gacctagcat catctcatcg tccgagtttt agcactatac
    12721 aacgtaaaga aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta
    12781 cccattggcg actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct
    12841 aacaaggtta ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg
    12901 caatggaatt tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa
    12961 taacaaataa gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga
    13021 taatttttta gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta
    13081 tttgttaaag taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc
    13141 atctaaagta acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg
    13201 cccttgcaga aaatcattca ctgtttcgta ttcttttttc atttcttttg aatctgtcga
    13261 tggtttatag cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt
    13321 gaaaatttct cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt
    13381 tcttggatcg atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg
    13441 tacttctagc aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc
    13501 atataaggat gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac
    13561 tgaatcagtt aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc
    13621 tgctgtttta tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct
    13681 tgtctcaaga atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa
    13741 ggataagatg ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac
    13801 tatatctttt gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat
    13861 cagcaacttg taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc
    13921 gccttctttc tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc
    13981 ttttttcatc ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga
    14041 atctgtgccg tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta
    14101 gtgatcaatt gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca
    14161 tatgctttct ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg
    14221 acttgttcta tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat
    14281 tctgtatagc ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc
    14341 gcttcctcaa aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg
    14401 acaagttcag cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg
    14461 atttctgctt gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact
    14521 tctgacccat cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg
    14581 atttcattga tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca
    14641 gccaaagcca ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga
    14701 caagaaacga cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta
    14761 atgttatata aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat
    14821 aatgagctat ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg
    14881 tatacgacca gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta
    14941 aaaaactcag catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc
    15001 caagcatgct ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg
    15061 atacaaactg gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa
    15121 ggctcattct cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt
    15181 acaactagtt cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct
    15241 aatttttctg attgataact aggatatttt tcttggaact ttttgacata ttcttctact
    15301 tgctgataca taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag
    15361 tataaacaat tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac
    15421 tatcttcaac gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa
    15481 cgctatccgt gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat
    15541 aataacgact gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga
    15601 ctaattcgtg tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc
    15661 ctggattgat caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc
    15721 ctaattcaag aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt
    15781 gataggctgg aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag
    15841 gaattcggat tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca
    15901 tatcctcttt tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa
    15961 tgtcaaatgt tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa
    16021 taatcactgg attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca
    16081 agaaagtact tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga
    16141 aagtgataga gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt
    16201 ggaatgtttt cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg
    16261 tacttgtttg cattttctta tccaaataat ccatgatgat catattaaat gtttctccag
    16321 aagttacttg ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca
    16381 ttttattatc gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa
    16441 tcggatgatc aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc
    16501 cattatccac gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac
    16561 cgttggtttg acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag
    16621 acatctcatt atcgtttact aatacgatta gattggattg taaaacacca ccattgttca
    16681 atccttcaaa agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc
    16741 ctgattcttt cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag
    16801 atgtgtgccc cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca
    16861 cttcacggta atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat
    16921 aagactgatg ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa
    16981 acgcaatcgt taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt
    17041 ccttttgcaa gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata
    17101 attttttgac atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa
    17161 tacattctct cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta
    17221 ttctactact tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct
    17281 tgttatataa gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat
    17341 taaaaagctt tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat
    17401 aggtgaagtt cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga
    17461 tagatctttg tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc
    17521 actctttttt tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata
    17581 ggatttattt cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat
    17641 tgtttgactg aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca
    17701 ctgttgatca aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt
    17761 tggatttgat ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt
    17821 tgttcttttt ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac
    17881 aacgtacgga caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt
    17941 tcagcgatac taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa
    18001 tttttataaa caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt
    18061 ctactgttga acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct
    18121 ttttgctctt ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc
    18181 ttatcttctt ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct
    18241 cgtatgataa aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca
    18301 taatcaatca actcgttaca agcttcttct acatacggat ttttattttc atagccttcc
    18361 caattgacat aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg
    18421 atttctttat ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt
    18481 gtatatactt tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata
    18541 ccattaaaat aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg
    18601 agctcagaaa gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact
    18661 tgttctttta tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt
    18721 ggtgtagtca cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat
    18781 aattgttcac acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt
    18841 cctgatacac gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt
    18901 tttgtttcct ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac
    18961 aaaagtcaaa caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact
    19021 tttaccccag cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact
    19081 acatctttca acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg
    19141 aagacaccta tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt
    19201 tttgcaggta caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt
    19261 gtcataattg atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa
    19321 ggagcaattc ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca
    19381 ttgagggagc attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt
    19441 tgattacctt tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa
    19501 tgatagattt cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac
    19561 aagattcgct caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc
    19621 gctcctgcat taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg
    19681 atagaattga aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt
    19741 ccgttgtctt tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag
    19801 cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata
    19861 cctaattgtt tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt
    19921 tgataatagt ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata
    19981 accagctttc attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt
    20041 aaatggaata actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg
    20101 ctgtccagct gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt
    20161 caaaagacct aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc
    20221 tctttcaaga ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta
    20281 caacatcagg tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc
    20341 cccgccgctt aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat
    20401 caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc
    20461 ttctgctgct acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat
    20521 gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat
    20581 gtgtcttaat aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg
    20641 aggtactgta gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac
    20701 caatacaaac attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact
    20761 ggcagatgtt tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg
    20821 caatttcgaa aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc
    20881 tgaagcgaca taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc
    20941 tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa
    21001 aatagcccag aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc
    21061 accaataccc gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc
    21121 aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt
    21181 gcgactgacc caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc
    21241 tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa
    21301 gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt
    21361 catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg
    21421 catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa
    21481 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
    21541 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
    21601 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
    21661 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
    21721 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
    21781 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
    21841 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
    21901 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
    21961 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
    22021 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
    22081 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
    22141 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
    22201 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
    22261 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
    22321 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
    22381 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
    22441 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
    22501 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
    22561 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
    22621 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
    22681 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
    22741 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
    22801 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
    22861 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aggcggattt
    22921 tataattaac ttagccattt ggaaataaaa aaacgccacg tgaattccat gttatattga
    22981 agttaccaca actctcaata gacaaggatg aattcacatg acgcaaaacc atgataacac
    23041 agtttcacgc aagggaaaac acctaaccta ctcagaacgt tgccaaatcg ccatcctgaa
    23101 aaaggaaaac tattccaacc gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat
    23161 aaacaacgaa atcaaacgcg ggaccgttac tcagcttaac cgccagaatc agaacgggaa
    23221 ggtctacgac tattatcatg acgtctacga cgccgatgcc gggcaggcgg cctacgagaa
    23281 gaaccgcttg aactgcggca gacggccctt atgggccgac acggacgcct tcattgaatg
    23341 ggcggacgac atgatgctgt tggaaaaatg gtccccggac gtggtggtgg gctttgccag
    23401 aaaacacgaa ttgttcgacc cggccattat ccctgggacc accaccctct atcagtggat
    23461 cgaccgtggc atcatgaaaa cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc
    23521 gaaatcgtcc cgtcatggca accgcccgaa taaacgggtg ctagggaaat ccattgaaga
    23581 gcgcccggag gcggttggta cccggaaaac cttcggccat tgggaagtcg acacggtcgt
    23641 aggaaacaag accaagtcgg atgccgtgct gctgacgttg gtggaacggc agacacgctt
    23701 cgaggtcatt ctgaagctgg ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca
    23761 gctgagagaa cgggctggtg cgtacttcgg ccagctgttc aagacggtta cctcggacaa
    23821 cggctcggag ttcgcgggac tgcacgatgc cttgcaggat gtggcaaaca tctacttcag
    23881 ccacccctat gcttcctggg agcgcgggac gagcgaaaac caacacaagc tgattcgccg
    23941 tttcatcccc aagggcagcc caatcggcaa cgtgagcgaa aaccaaatct tgcggattca
    24001 gcgttggatg aacgattacc cgcgaaagat tttgggctat gcaacgccgc atgacacgtt
    24061 cgtccaggca tttaggcagg aacggttgac ggcttgacac gtgggccctt gacaacgagc
    24121 ctccctcggc atggcttgtg tctggaaagc tggccggggc cagccagtcg ggagactacc
    24181 atgcctcagc tcattgtcaa gggcccactc gattagaaaa attagacggt tggtggtaac
    24241 gagcaaccgg gtcaccatgg gtggctaact taaacttgaa atttacgtaa ggaataaaga
    24301 atacttcata taaaatatat aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt
    24361 tgttcttcat ttcaacataa gtttttcttc aatttgctta taatttacgt gcaaagaatg
    24421 aaacgactaa tactaaaatg attgcaccga tcaatgatgg aatgatagcc attcctgcaa
    24481 ttgttggtcc ccaagttcct agtaaagctt gtccaattgc tgaacctact aaaccagcaa
    24541 tgatgtttgc gatgatcccc attgatgatc ctttattcgt gattgctcct gcaattgctc
    24601 caatgatacc gccgacgatt aatgcccaaa taaatcccat tttaaattcc tcctaactga
    24661 cttcttactt cttattgtac acgtggttcg ttattttctt tgactttttg tgcacctttg
    24721 cctacagcag attttgcttt atctgtttgg cgtgaagcaa attgtcctgt ggattttgct
    24781 gcttctgtta ctttgtcttg tactgtttct tgatcttttt ggtattcttc ttgtgttttg
    24841 atgtctgcaa cattcacatt cacttcgata acatctaaat gtgtcatatt gtttacttca
    24901 gtactgatca attctttgat ttggttgtaa atatcttcga tgtctttacc gtattctacg
    24961 atcacatcta gatccacagc tacttgtttc ttacctactt ctgtattgat acctgctgtt
    25021 acgttatctg tgttgactaa tttttcagcc atatttgaga aaaatccgcc atctaatgtc
    25081 aatagaccat ctacatcttt caatgcataa ccgatgattt tttggatgac tttgtcttca
    25141 taagtcaatt caccattcat catgcggttt tctccttttt catttgccaa aactgatcca
    25201 tttgttgtat tcatgttttt gttttccatc tttgtttcct cctatgattt aaaaaattga
    25261 tcaactattc ctgactgttt caaataaaaa ccgattcctg cgccaattgc tgtcatgatt
    25321 atgatcaaca atgtcttgaa aaatccaatc gttaaccata aaacagctag cagtagtcca
    25381 agacccccaa agataatagg gagcttgtat gctgaaaata gttctttcat tatttggccc
    25441 tcctattcca ctcgagagtg cttgtagtct cttgtatctt cttgttcttc ataaccgctg
    25501 tatgtgactg ccactttgac cgtttctttt gttccgagta ctttagttac ttcttcttcg
    25561 atatcttgca tcaatacacc tgttttccct actaatgaag aagttcgttt caagtctccg
    25621 ttgacatgga tatggattcg atttttcgtt gaacgtatcc gtactttagg tgaatgaatg
    25681 aactcttctt cgtgtaaatg ggaacgtacc aacccttcga tcgctttttt gtcgattgtt
    25741 agtttgccat cttcccgttt cataacgaaa gttcctcttg actttggata gaaaagaacg
    25801 actagcaaga tgataatagc aagaacggca aatactacag ctgcccagaa aatatattgt
    25861 tgcatgtagt aattcgttaa agtaaagaag cgaaatcgtt ccaattgttc aggcataata
    25921 tacggcgcat tgatcaacgc aatcgctaga aatactgaaa gtagcagtaa actcatgata
    25981 acaccgatta tttttaaccc cttattcatt ttccacctcc tataaactta tttttaccct
    26041 gtaaataact ctaccgacaa tcacagtata gatagcgaac agttagcata gcatcagtaa
    26101 ataaagcgct accaaacatt gctggatcat aacggcgttt tattgccagt ctgatactga
    26161 aataacaatt gcttacttat tctctattta cttttgattg ctggctatac ttaaaggata
    26221 ttattcaaga ttaaaaattg caaagattat gctcagttat tattacaaga ttctatttat
    26281 t
//
LOCUS       DASJTM010000037        25615 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJTM010000037 DASJTM010000000
VERSION     DASJTM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.037"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..599
                     /locus_tag="U4B29_001417"
     CDS             45..599
                     /locus_tag="U4B29_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2106372.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(772..1245)
                     /locus_tag="U4B29_001418"
     CDS             complement(772..1245)
                     /locus_tag="U4B29_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2106373.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1257..1793
                     /locus_tag="U4B29_001419"
     CDS             1257..1793
                     /locus_tag="U4B29_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2106374.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1833..2204
                     /locus_tag="U4B29_001420"
     CDS             1833..2204
                     /locus_tag="U4B29_001420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2106375.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2488..3759
                     /locus_tag="U4B29_001421"
     CDS             2488..3759
                     /locus_tag="U4B29_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2106376.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4004..5029
                     /locus_tag="U4B29_001422"
     CDS             4004..5029
                     /locus_tag="U4B29_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2106377.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5323..6432
                     /locus_tag="U4B29_001423"
     CDS             5323..6432
                     /locus_tag="U4B29_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2106378.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6515..7411
                     /locus_tag="U4B29_001424"
                     /pseudo
     CDS             6515..7411
                     /locus_tag="U4B29_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7427..8251
                     /locus_tag="U4B29_001425"
     CDS             7427..8251
                     /locus_tag="U4B29_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2106379.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8283..9593
                     /locus_tag="U4B29_001426"
     CDS             8283..9593
                     /locus_tag="U4B29_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN2106380.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9741..11000
                     /locus_tag="U4B29_001427"
     CDS             9741..11000
                     /locus_tag="U4B29_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2106381.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11010..11882
                     /locus_tag="U4B29_001428"
     CDS             11010..11882
                     /locus_tag="U4B29_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2106382.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11894..12724
                     /locus_tag="U4B29_001429"
     CDS             11894..12724
                     /locus_tag="U4B29_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2106383.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12764..14947
                     /locus_tag="U4B29_001430"
     CDS             12764..14947
                     /locus_tag="U4B29_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2106384.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15008..16228
                     /locus_tag="U4B29_001431"
     CDS             15008..16228
                     /locus_tag="U4B29_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN2106385.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16225..17685
                     /gene="gtfA"
                     /locus_tag="U4B29_001432"
     CDS             16225..17685
                     /gene="gtfA"
                     /locus_tag="U4B29_001432"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN2106386.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17762..18868
                     /locus_tag="U4B29_001433"
                     /pseudo
     CDS             17762..18868
                     /locus_tag="U4B29_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19190..19399
                     /locus_tag="U4B29_001434"
     CDS             19190..19399
                     /locus_tag="U4B29_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2106387.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19392..19541
                     /locus_tag="U4B29_001435"
     CDS             19392..19541
                     /locus_tag="U4B29_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN2106388.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19695..20360
                     /locus_tag="U4B29_001436"
     CDS             19695..20360
                     /locus_tag="U4B29_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2106389.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20644..21729)
                     /gene="xerS"
                     /locus_tag="U4B29_001437"
     CDS             complement(20644..21729)
                     /gene="xerS"
                     /locus_tag="U4B29_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN2106390.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22063..22296
                     /locus_tag="U4B29_001438"
     CDS             22063..22296
                     /locus_tag="U4B29_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN2106391.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22293..22700
                     /locus_tag="U4B29_001439"
     CDS             22293..22700
                     /locus_tag="U4B29_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2106392.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23837..24355)
                     /locus_tag="U4B29_001440"
     CDS             complement(23837..24355)
                     /locus_tag="U4B29_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN2106393.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25473..>25615
                     /locus_tag="U4B29_001441"
     CDS             25473..>25615
                     /locus_tag="U4B29_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2106394.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQS"
BASE COUNT         8723 a         3923 c         4787 g         8182 t
ORIGIN      
        1 tacataatca ttaactcaga atatttttaa gaagggaaac aatagtgagc tataatgtac
       61 aaccgttaag gacaaatgaa gagatacaag attttcagtt ttggctaaga agaacaagta
      121 atccggaaag agatagtttt ttatttttgt ttggaatcaa taatgggtta cgtatgtcag
      181 atattatagg attaaaagtt ggagatatac gaggcaaaag taagcctatt attgttgaac
      241 ataaaacagg taagcgaaaa ccgattttta tagataattt gagagaagaa attttattat
      301 atactgaagg taaagaagaa aatgattggt tatttccgag tcggcaacaa ggtcgacata
      361 taacaaggga tcgtgtctat cagatatatg cagatatagc agaaaaatta ggacgagatg
      421 atataggaac acatacatta agaaaaactt ttgggtatca ctactacaag aaaactagag
      481 acattgctac cttaatgttt atttttaatc atagctcgca agcaattaca aaaaggtaca
      541 ttggtataac tgaagatgaa ataggagcat cacttcgtgg atttaaacta ggtgtataat
      601 ttaaaaattt ttataaacag taaagacaaa gattaaaagc tatttttatc tagaacgatt
      661 ttatttcata aagttcgcgg aataaaagct ggggttactg gtacttggta taggttcgtg
      721 ttatataaac taagtaatta aataacattt ttaggttgga aaaggatgaa attaaaaaaa
      781 gagaatcaat acggtctcaa aaacgcaaac gctgcggaat aagaaaaata ctgataactc
      841 aacgtttagc agactatcta atagggatag tctgcttttt tcgtttttga ccatactttt
      901 gaccttctgc aaacttcctt gcttttcctc tgtagaactc atgaaatctg caaaacgaat
      961 tgccgtttcc tctgtctgct ttttcgcgat atgggtgtat atatcatcca tagtggtttt
     1021 aatatctgta tgtccaagtc gaacatacac ttctttgaca gacgcaccag attcaaagag
     1081 cagactacaa tgggtatgac gaaaaccatg aagtgtgata tgttttaagt tattctcttt
     1141 aatgatgatt ttcaaattgt gatttacata gtctaaataa aggggcttat tttcttctga
     1201 tgaaaacaaa agttgttcac catcttttat aggtttgatt ctcattttta gatattatgc
     1261 ccgcacgtat tcatcacatt tctattgtga atcgctttat tcgaccgaca tttgactttt
     1321 atcataatat actcggttta aaattactaa tgaaaacaat taatcaagac gatcatacca
     1381 tgtatcacct gtttttttca gataatcacc accgtgtggg aactgaactg actttttttg
     1441 aagtacaaga agggaacaac cagttttttg gaaccaatac gattgaacga acgatactaa
     1501 aagttccttc cgaagcatca ttgcattttt gggaaatata ttttgaaaca caaggcgttt
     1561 gccattatgg aatcgaatca ttttctggac gacctatctt acgttttgaa gggccagatg
     1621 caacacgatt agctttagtt cccttacgcg aatttgaaga tattgatgat tatttcccgt
     1681 atgtcacaga ccagatccct acagagcatg ccattttagg aattgatgcc attcaactac
     1741 gcgtacaata ttccgatgcg actgaacgag agttattgtc ggtaggctgg tagcacaaag
     1801 aatacgtcag tttcttttcg ggtcatcaac gagtgacaat cttagaaaat caagacactc
     1861 agttctatca agaagttcag attattcaag atcgtgagaa tccagttgcc attgaaggga
     1921 ttggtagcgt ccatcatgtt gcgtttggcg tagaaaataa atctgattta caacgcatcg
     1981 acaaacaatt acaagagcgc aattttataa attcaggaat aaaagatcgt gaattttttg
     2041 tatcacttta ttatcgtgac ccaaatcaat tattgattga aattgctaca ggggaaggaa
     2101 acctagatgc aaaagcgtat gaaaatcaaa gtccacgctt tgaagaaatt cctttatttt
     2161 taccgcagta cttaaatttc attcgtgaac aagtggagca ataattagct aatcaaatcc
     2221 atgacgaaga gtaagaggtt gactcataag tcaaaaaata aagaaaaacg catcaaatcc
     2281 attctaaaga atgttgattt gatgcggttc ctatttttat aaactatcat tttcgactaa
     2341 taaaactact tatgagtcag cctctctttt tttcttgatt tttatacctc tttctaggta
     2401 aactaaacat ctgccaaaaa taccgtgtct cattgaaaca ttctcaacta tttcaaaata
     2461 aaggggagta ccaaatgaac gagtatattg aaattatcca cgcaagagaa acaacttaaa
     2521 aatgtcagtc tcaaaattcc caaaaataaa attagtattt ttaccagcgt atctggttca
     2581 ggaaaatcat cgattgtttt tgatactatt acccaagaag ctggtcgtca actgaatgac
     2641 acctatgata gttttacccg tttattttta cctaaataca gtcgtccaga ggttgatgag
     2701 attgataatc tttctacggc gattgtggtc aatcaaaaga agattggtgg cagtgcgcgc
     2761 tcaacgttag ggacgattac taacatcaat gcgttatttc gtgtgttgtt ttcatgtttt
     2821 gcaacaccta gtttaggata tgcaaatgct tattcattta atgatcctag tggaatgtgt
     2881 tctgtctgtc agggaatcgg taagacgatt gcatttttaa taggattgct ttatttttcg
     2941 gctattgata atgctgattt tgtattttct gatccattta ccgcagaagt tttgtctgga
     3001 attgaggaac aggcaaagaa tcatggttat tttgtattgg tgcataatgt taattcagca
     3061 gaagatgtta aaactattca gagaaattgg cgttttgagg ggttcattgt tgttggtgta
     3121 cgtgtaaaag aatttttgga attaaataac gtttttgatg gtcctgttgt ttatattgat
     3181 acgcatcttt caaaagagac attaagagaa cttgaacaac aaatgaatcg tttatttgta
     3241 aatacaaatg attaccaagc tagtacattg gcagtggaac atttgattca ttatggtcat
     3301 gagaaaatag cgtttctttc ttttgactac attgtaggtg aaccaagtgt tattcaagaa
     3361 cgacatcaag cttatatcga tacattaaag aaatatcatt taacctttaa atcgcattgg
     3421 atatataaca actctgaatt tgagcgaatt tatcaagaat tagctgaatt tactgcaatt
     3481 gttgttactg gagattattt agcggctaaa ttagtaaaat ttttaaaaaa taagaataaa
     3541 cgagtgatgg aacaattatc gattattgga tttgatgata ttacttttgc agaattaatg
     3601 gatcctgcgt tgacaacaat acgactaaat ccaggtaaca agggggcgat tgcttttcac
     3661 caactgattg gactggccaa acaagaagaa ataaaaaatc aagtactact attagaggga
     3721 gaattgattt ctcgagactc tgttcacaaa atcaaataaa aaagctctgt gaaataagta
     3781 tagttaacaa taaaatatcc gaacgataga gagaatgaac tgtgatctag cagttcctcg
     3841 tcgcaaattc actagaatta gcattattta ctctaaattg ttcggatatt ttttcttgtt
     3901 tttaattatg attacagtgc ctatttttaa cgaaatttgc ttattttcaa aatgtatgat
     3961 tatatgttag taaacacttt caaatatgaa aagaggagct attatggtag gaattaggga
     4021 tgttgcaaaa cgggcgggtg tgtccattgc aacagtatcg cgcgtgctaa atgaggatcc
     4081 gacactttca gtaaccgatg aaaccaaacg aaagattgca gaatcggtta atttttatca
     4141 ttatgtcaaa aaaagtcccg ttgcaaaaaa tttaacaaat attgcattaa taacgacagt
     4201 ttctgaagta gatgagttgg aagatcccta ttttcgaacg attcgcagag ggattcaagt
     4261 cgaagcagaa caaagcaaag tcaatttgaa gaaaattatt cgtttgtcag aaacaccgtt
     4321 ggagcaagac gatttgctaa aatgtcaagg tgtgttgatt attgggcagg tgctatcttc
     4381 tgtgattgaa gaagtatcag caattaattc gaatgtcgta gtgattgatg accctagtat
     4441 tgaagctgaa gtagatgcag tttatactga tttaacaaaa gcaacgaatc agcatttaga
     4501 tcgattgtat aaaaaaggac accgaaacat tgcgtttatc ggaggaggac gtgttcgttt
     4561 agatagccaa ggaaataaac acgtgtcaga tgatgattat cgaaaaatag cctatgagac
     4621 gtggatgaaa caaaaaggat tagaaaaata tagtcaaatt tatttagaag gctggaccac
     4681 acttgacgga atgcaggcag cagatttact tttgaaaaaa catgctaaag ataagttgcc
     4741 gactgctatc atggtaggaa atgatcctat tgctgtgggg gtttatcgtt cacttcaaaa
     4801 aaatggtcta catattccag aagatatttc gattgtgagt tttgataata ttgaagttgc
     4861 tgaattttta acacctgcat taaatacggt gaatgtgaat acagaagaaa ttggaaaact
     4921 agcagttcgc atggttaatg aacgaataaa agaagttaga aatgtttcga tcaaagtgat
     4981 tgtttctaat aatattattg ttagagaaag tgaaaaaaat atcgattaaa aaagtaaaca
     5041 tccaaaaaag ggtgtttact tttttgttta ctaatatttt tagtaaaaaa tctcgacaaa
     5101 acattaagta aacagttatg gtgttgttaa caaaaagaaa acgcattcaa aacaggagga
     5161 attaagatga aacatttcaa aaaaatagct ttaacaggga tagctgtttt aactgggaca
     5221 ttactcgcag catgtggtgg gggagatagt aattctaaag acgggcaagt aactttaagc
     5281 tatgcgagtt gagatactga ccaagctcca ggactaagaa aaatgttaga tgaatttgaa
     5341 aaacaaaatc ctgatatcaa agtagaaatg gagacaacgc cttgggatca atattgggtt
     5401 aaattagaag ctgcaacaac gggaggtaat atgccagatg tagtaaccat gcactctagc
     5461 gaatcctata aatatatgtc acagaatgct ttgatgggac ttgatgatgt tatcaaagaa
     5521 aatgatattg acatgggaaa ctttactgaa ggaattgctg atttctatac gcttgaagac
     5581 gaactatacg caatccctaa agatgcttct gtagtggggc tatggtacaa caaagaatta
     5641 tttgataatg caggtgtcga ttatccagat gaaacatgga catgggaaac actcagacaa
     5701 gctgcaattg atttaacaga tgaatcaaaa ggcatctatg gttttgcatc acatattgga
     5761 acagagactg gctactggcc atttatttat caaaatgggg gagaagtatt tgccgatgac
     5821 aatacaaaat caggtttgaa taccccagaa gcacaagaag ctttagcttt ttatcgagat
     5881 attgcacttg ttgatcatgc ttcaccaaca attacagaat tacaagatcc tgataagtta
     5941 agtcgttttc aatcaggtag attagcaatg atgattgaag gaaactggtt tgcaccggga
     6001 tttttgaaaa atgactatct agttgaaaat gttgcttctg ccgtgttacc taagggaaaa
     6061 gttaatgcaa caattacaaa tggacttggt tgggctgcca gtgcgagtac aaaataccca
     6121 gaagaagtga aaaaactaat ggcattttta gctagtgagg acgccaataa aatccaagcg
     6181 gaaacggggg catcaatccc agcactaaaa ggctttgaag acgagtgggc tgcttcttca
     6241 gatacaattg atttgagtgc ttttggaaaa atgttggagt atggagtccc tcgtccatac
     6301 aataaagatg gattacgagc tgaacaagca gaagttcaaa ttattaaccg tattctgtct
     6361 ggtgaagtaa ctgttgaaga tggaaccgct caaattgaaa aagaagtcaa tgcgatattg
     6421 agcggaaatt aaattagtta attatacaat atacaggcga aaggattcta tttcgtctgt
     6481 atattaatgg atgaatacca aaaaggggga tattatgaaa aatcgaattt ttggaaaata
     6541 tcgtaaagat caagtgatat ggggctatat ttttattttt ccaacggcat taggattaat
     6601 tttattaaat atatggccag caattcaaac agcaatttta agttttcaaa aaacggtagg
     6661 gttcggtgat actgaatggg tcggattaag aaattaccaa aaaatgtttg cagacggtga
     6721 agtttttcag tctttattaa acacattaat ttatgcagtg gtttcgatac ctagtattgt
     6781 tgtattatcc ttgttggtgg cagttttaat gaatagaaaa gttaaaggat tgtcgattta
     6841 tcggacaatt tattttctac cagtcgttgc tgcaccatcc gcagtcgcaa tgatttggcg
     6901 ctggatgttc aataatgatt atggactgat taataacatg ttggctaaaa taggtctcga
     6961 tggaccagca tggttaacgg atccatcgat tgcgatttat tctattatca ttgtaggagt
     7021 ttggagtgct attggttata acatggtgtt attactagcc ggcttataag aaattcctaa
     7081 agattattat gaagcagcgg aaattgatgg tgctagtcca atccagcagt ttttcaacat
     7141 tacgttacca ttagttacac caacattatt ttttgtagtt gtaacaacag ttatcaatgc
     7201 tttccaagta tttgatgtta tttttatgat gattactcct aatagtaccg ccatgtttaa
     7261 atcacaatca ttagcctatt tattctataa acattcattt attttaaatg acaagggata
     7321 tggttcggca atcgttatgt tccttttagt gattatatta attattactg caattcaaat
     7381 gaagttgcag aaaaagtggg taaactatta aggaggctat caaattatga aaaataaatt
     7441 tttttcaaat gcgccaatcc atatcatgct tattttaggt ggtattgcaa tggcattacc
     7501 ttttatttgg atgattttaa cttcagggaa aacgttggtt gaatctaccc aaattccacc
     7561 tacaattttc cctgagaaat ttcagctaag taactatcaa caagtacttg atttattacc
     7621 attcatggct ttttatatca atacattttt aatggttgtt ggacgagtga ttggctcggt
     7681 tttattttca gcaatggctg catatgcttg tgcgcgtcta agttttccag ggaaaaatat
     7741 ccttttcagc ttagtactta ttcaaatgat gattcccggt tccattttta ttactccaca
     7801 gtatttatta gcacaaaaat tgggtatttt aaatacaatt ccagcattaa tattaccagg
     7861 ggtagttagt gcgtttggta cgtttttatt aagacaattt ttcttaggtt tacctgatga
     7921 attggaggaa gcggcctatt tagaaggtgc caatatttgg acgatctttt tcaaaattat
     7981 gttgccgctt gctaaatcag gattggttgc acttggaata ttcacagcgt tgtttgcatt
     8041 taaagattta atgtggccat tgattgtcaa tacttcaatt gataagatgc ctttagctcc
     8101 gggattagca aacttacaag gacaatatgc cacgaacttc ccacaattaa tggcaggttc
     8161 gttattagca atttggccaa tagttgtgat attcatcgtt ttccaaagaa aattcattga
     8221 aggaattgct acaacaggta gtaaattata aaactattaa ttagtgattg gagagtaagt
     8281 acatgacaaa aattacattt attggtgcag gaagtactat ttttgcaaaa aacgtattag
     8341 gcgatgcgat gttaactcca agtctacaag atgcacatat tgcattgtac gatattgatg
     8401 aaaaacgact aaaagaatca aatttaatgc ttaaaaatat taataaaaat tcgaatgaaa
     8461 atcgtgctga tattgtttct tattatgatc gcaaagaagc gttaagagag gctgattttg
     8521 ttatcaatgc tattcaagtg ggtggttata aaccttcaac agtgattgat tttgatattc
     8581 ctaaaaaata tgggttgcga caaactattg gtgacaccat ggggattggt gggattttta
     8641 gaaccctacg gacactacca gtaatgttag actttgctaa agaaatggaa gaagtttgtc
     8701 caaatgcttg gttattgaac tatacaaatc cgatggcgat gttaaccatg ggtgttttaa
     8761 aagcgacgag tattaagact gttggtttat gtcatagcgt tcaagtatgc gtaccagaat
     8821 tgtttgagca tttaggaata gccgatcaat atgacttaaa tgactttcaa tggaaaattg
     8881 caggcattaa tcatatggca ttcttgttgg aaattacaaa aaatggggaa gatttctatc
     8941 caacaattcg tcagttagct gcagaaaaac caaatccaca tccagattct gtacgttttg
     9001 aattgttgaa acgttttggt tactatatta cagaatcaag tgaacacaat gcagaatacc
     9061 atccatactt tattaaatca aaatacccag aattgattga cgaattaggg attccgttgg
     9121 atgaatactt acgtcgttgt gaacaacaaa ttaatgattg ggaaagcatg cgagaggaca
     9181 ttgtaaataa tggggagtta acacatacga gaagccgtga atatgcttca tatatcatgg
     9241 attctattac gacaggaact ccgtcagtga ttgcaggaaa tgtcttaaat aaaggcttaa
     9301 tcacaaactt accgacagat gtttgtgtgg aagtaccttg tttagtcgat aaaaatggcg
     9361 tgcaaccaac ctatgtcggt gaattgccaa cacaattagc tgctttaaat cggacgaata
     9421 tcaatgttca agaactgaca gtagaagccg ctttaacact agataaatca aaaatttatc
     9481 aagcagcgta tttagatcca catctatcat ctgaattgtc tttaacagat atcaaaaatt
     9541 tagtggatga tttaatcgaa gcacatgggg actatttacc tgaatataat taaaaagaag
     9601 ctgaaagagt aaggaatttt caattcctta ctcttttctt agttaaattt agtaaaaata
     9661 ttgacaaaac acttagtaaa cagttacatt agtgttaaca aaaagaaaac gcattcaaat
     9721 tgtataagga ggagcataaa atgaagtggt ctaaaaaaat tattttagct tgtatagcac
     9781 taggagcaac gatctctttg actgcttgtg gaaaaaatca ggaagatgca agcggtaagg
     9841 tagaaataga gttttttaat caaaaaaaag aaatgactca aacgattcag gagattgcta
     9901 aagattttga agcaaaaaac cctgatattc atgtaaaagt tgtggatgta ccgaatgctg
     9961 gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc agatgtcatt aatctttatc
    10021 ctcaaagtat agaattgaaa gaatgggcga aagctggtta tttagaggac ttaaccgaag
    10081 aaccgtattt agagaatatc aaaaatggat atgcacaacg ttttgctatt gaggatcgtg
    10141 tatatagcat accattaacc gctaatgtct atggttttta ctataacaaa actgcttttg
    10201 aggaaatggg gattcaagca ccagaaactt gggcagaatt tgaaaaaata gtagctgaga
    10261 ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc agaaggctgg actttaaatg
    10321 gttatcacca actagctata gcgactgctg ctggtggtga agaggaagcg aacaatgttt
    10381 ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa agaaatgcaa aaagatttta
    10441 atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa caactggcaa ggtgccggtt
    10501 ataacgatac agtagttact tttacaaaag gtgatgcgtt gattatgccg aatggttcat
    10561 gggcaatgcc gatgattcat tcacaaaatc cagattttga agtaggaact tttccttttc
    10621 cagcagatga agcaggtcaa agtctaacga ttggcgcagg agacctagcc ttatcagttt
    10681 ctgcaacttc tcaacataaa gaagaagcca aacgttttgt agaatatatg acaacacctg
    10741 aagcgatgca gaaatattat gatgtagatg gtgcaccgtg tgctgttgag ggcgttatcg
    10801 agaatactca ggattcacca ttaagtggtt taactgaact tgcttttact gatcgtcatt
    10861 tagtttggtt agccaaagat tggaatagtg aaaatgattt ttatacctta acaacaaatt
    10921 atttacacaa tggcaatcaa aaagccatga ttaacgcatt aaatgctttc tttaatccaa
    10981 tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa agaaatttta aatcgttact
    11041 gggcaatcat ttttgtagca attccaatta ttttgcagat gatttttttc tactttccaa
    11101 tggttcaagg tgctttttac agtttaacta attggactgg tctgacttat aattttgatt
    11161 ttgtgggttt gaacaattat aagattcttt tttctgatcc gaagtttatc aaatcgattg
    11221 gcttcacttt aattgtgacg ctttgcatga ttgctggaca aattggtttt ggtattttga
    11281 ttgcgcgggc gttgaacgca aatattaaag ggaaaacatt ctttagagca tggttctttt
    11341 ttccagcggt cttatcgggc gtggttgttt ctttagtatt taaacaaatt tttaactatg
    11401 gtttaccaaa aattggtagt atgttaggca ttgaattttt acaaaccagt cttttaggaa
    11461 caacatgggg agcgattttt gcagtaattt ttgttttact ttggcaagga attgccatgc
    11521 cagtcatcat tttcttagcg ggttttcaaa gtattccaca agaaattatt gaagctgcta
    11581 gaatggatgg ggcgaatgat aagcaaattt tttggaatat tgaaatcacc tacttattac
    11641 caagtatttc aatggtattt attatggcat taaaaggtgg cttaacagca tttgatcaaa
    11701 tttttgcttt aactggtggt ggaccaagcg atgccacaac atctttgggg ttacttgttt
    11761 ataattatgc ttttaaaaac aatcagtttg gttatgcgaa tgctattgct ttagtcttat
    11821 ttatcttaat tgggattgtt tcactggcac aattaaaatt atcgaaaaaa tttgaaatct
    11881 aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt tctaaatact tgcttttatt
    11941 attagggggc ttcatgattt tagttccctt agtttcaacg ctattcggtt catttaaaac
    12001 aacacccgat attatgaata atttctttcg ttttcctaat ccagcgactt tggaaaattt
    12061 ccgaagattg ttagcggatg gaattggtca ttacttttgg aattcggcaa tcatcacaat
    12121 cattgcagta gttgctgtga cagcaattat tcctatggct gcttattcac tagcaagaaa
    12181 aatgactcgt gggaaagcat acggattgat gtatttattt ttaattttag gaatttttgt
    12241 tcctttccaa gtaattatga ttccgattac cgttatgatg agtaaaattg gtttagcaaa
    12301 tatgtgggga ttaatcttac tctatctcac ttatgccgta ccacagacac tatttttata
    12361 tgttggtttt attaaactaa atgtgcctat cagtctggat gaggctgctg aaattgatgg
    12421 ggctagtcac tttacaactt atttcaaaat tgttttccca atgttaaaac caatgcatgc
    12481 gacgactatt attcttaatg ctctatggtt ttggaatgat ttcatgctac cattattaat
    12541 tttaaatcgt gattctaata tgtggacact accattgttt caatataatt atacgggaca
    12601 atattttaac gactatggtc caagttttgc ttcctataca gtaggaattg tcacgattac
    12661 actagtttat cttgctttcc aaagaagtat tatagctgga atgagcaatg gagcagtgaa
    12721 ataaccaata aattattctc attataaagg agttcataaa cttatgatta tatttgacaa
    12781 tcacacacaa acatttcatt tacaaaataa aaaaattagc tatctattag gaatcgaaga
    12841 aaataattat ttaacgcatc tttattttgg aaaaaagatt acgcactatt ctggtgggta
    12901 tcgctacccc cgaacagatc gttccttctc acccaacaat gcaaatgcgc aagatcgttt
    12961 attatcttta gacacattac cattagaatt tccaggatat ggacatggtg attttagaga
    13021 accggcgcat aatattaaat taggaaacgg tagtcgtgta aatgattttc gttatgatag
    13081 ctacgaaatt caaaaaggaa agcccagctt agcgggattg ccagccttat atacggaaga
    13141 tgatgatgaa gctgaaacgc tgattattca attgaaagac gcgattagtg gattgacttt
    13201 acagttgttc tacacaattt atcaaaatca cagtgtaatt attcgttcta cgcaattagt
    13261 aaataatagt gatgaaacag tggagattaa ccgcttagct agtcaatcaa ttgattttcc
    13321 aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg gcaagagaac gtcaaatgac
    13381 tagagaaaaa attcatacag ggataaaagt attagatagt aaacgcggat caagcagcca
    13441 tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca gatgaatttc aaggagaggc
    13501 atatggattt aatttagttt atagcggtaa tcacgaaacg gtgattcaaa aagatccatt
    13561 tgaccaaaca cgggtcatta tgggaattaa ttcatttaat tttggttggc aactaaaaac
    13621 tggcgactca tttcaaacgc cagaagtcgt gatggtttat agtaatgaag gtctgaatac
    13681 gttatcacag acgtatcatg aattgtataa tcaccgttta attcgtggaa actatcgttt
    13741 aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg tattttgatt ttaacgaaga
    13801 aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc attgaaatgt ttgttttaga
    13861 tgatggttgg tttggcggtc gtgaaaatga cttcacttca ttaggtgatt gggttgaaac
    13921 agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag caagtgcatg ataacgggat
    13981 gaaatttggg atttggtttg aaccggaaat gatttctgaa gatagtgaac tatttcgagc
    14041 acatccagat tgggcattgg gtattccagg tcgtgaccgc tcaattagtc gaaatcaata
    14101 tgttcttgat ttttcaagac aagatgttcg tgataatatc tatcaacaaa tgacggcgat
    14161 ttttgatcgt gtaccgattg attatgtgaa gtgggatatg aatcgaaata tgacagaagt
    14221 ctattctgtc ttgttggact caacgatgca aggtgaagtg gctcatcgtt atatgttagg
    14281 gttatatgaa tttttagaaa agttaacaac gaattatcca actattttat ttgaaagttg
    14341 ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat tacatgccac aaacttggac
    14401 gagcgacaat accgatgccg tggctcgtct gaaaattcaa tatggaacca gcttagtgta
    14461 tccaatttca agtatgggtg cgcatgtttc agcagtccca aatcatcaaa caggaagaga
    14521 aacgagttta actattcgtg gcaatgctgc gatggctggt gtttttggtt atgagctaga
    14581 tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa gaacaagttg ccttttataa
    14641 aaagcatcgc caacttttgc aatatggaac tttttatcgc ttggaaagtc cgtttgaagg
    14701 caatgatact gcatggatgt ttgtctcaaa agaccaaaca gaagccatgg tcttttattt
    14761 ccgagtatta gcagaagctg cgcatccatt agtcacattg aaattagcag gattagcaga
    14821 agaagcaact tatcaaatgg aagatgtaac atttgctggc gatgaattga tgaatctcgg
    14881 tttttatatt gactcagaac tacatggtga ttatgcgaca caacgctttt atttgaaaca
    14941 gatatagtag cttgaattta caatataagt gcaacaccaa aagagactca ttccaaacga
    15001 agaaaatatg ttttatttac acgcatttac taaaaagcag cctgatttga attgggaaaa
    15061 tgaagaagta cgtgaagaaa tctatgcaat gattaactat tggttagata aaggactagg
    15121 cggatttaga atcgatgcga tattaaacat caaaaaaaaa ctagagtatg gcatctttga
    15181 atctgatgga gaagatgggt tagccttcat tgggccctgg attcttaatc agtcgggcat
    15241 tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa ccacataata gtatgacggt
    15301 agcagaagcc gatgttccta atgaacgttt agatgaatat attggtgaag atggctatta
    15361 ttcgatggtt tttgatttta gttatacgga tattgatgta cctgaaactg gtgaatggtt
    15421 taaagatagc caatggacat ggacggatat gcgtaccaat atttttacga atcaattagt
    15481 tacgcaagat aaaggttggg gcgccttata tttagaaaac cacgatcaac cacgttcaat
    15541 taataaatat attccagaag aatggatcaa tgattatagt aaaaaaatgt tggcgacgct
    15601 ttttatgtta ctaagaggta ccccttttat ttatcaagga caagaactag gaatgaccaa
    15661 catagaaatg gcatcattag aagattttga tgatgtagct acgcattctc agtataaacg
    15721 ggcattagaa tatggattgg caccagagca agcattgaaa gctgtcaata aacgaagtcg
    15781 ggataattct cgcacaccta tgcaatggaa tacgcaaaaa aatgccagat tctcaacttc
    15841 tgataatgta tggttaaaag taaatcctaa ttatccagaa ctcaatgcag aagctcagtt
    15901 aaccaatcaa cagtcggtat tcaattatta tcatcaatta attaaattaa gaaaaaatag
    15961 tgtatacaaa gacgtgctaa tctacggaca atttctacca gtgaaagata aaaatgaaca
    16021 tattttgctt tatgaacgcc aattagaaga taaaatcgta ttagttatct taaacgttac
    16081 agcaacagaa caagaatatt atgtagatga acagtaccaa caagtattaa tcaataatta
    16141 tgaagatgtt ttattagatc aaagtcgaaa attgcgttta cgaccgtttg aaagtattgt
    16201 attagctaat tatggaggac aattatgaaa attaaaaacg aagcaatgct catcacctat
    16261 gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag tcttagacaa acatttacaa
    16321 ggtgttattg gtggtgtaca tctgttgcct ttttttccat cgacaggtga tcgaggcttt
    16381 gcgccgagtg attatacgac agttgatcca gctcttggaa catgggaaga tgtggaagca
    16441 ttaggtgaag attattattt gatgtttgat tttatgatta atcacatttc tcgtgaatca
    16501 aaatttttcc aagatttcaa aaaaaaccat gagcagtcac catacaaaga aatgtttatc
    16561 cgtattcatg aatttttccc agaaaatcga ccaacacaag aagatattga tttaatctac
    16621 aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg cggatggtac gacagaagag
    16681 gtttggaata cctttgggga agaacaaatt gatttagatg tgaccaaaga agttgtgaaa
    16741 gaatttattc gagaaaccat caaagatatg gcgaaccatg gctgttcttt aattcgatta
    16801 gatgcgtttg cttatgcgat taaaaaatta gataccaatg atttcttcgt ggaaccagat
    16861 atttgggaac ttttagacga agtgcgtcaa gaagcagcga aatatgacgt cgaattatta
    16921 ccagaaatcc atgagcatta ttccattcaa atgaaaattg ccaatcatga ttattatatc
    16981 tatgacttcg ccttgccaat ggtcactctg tattcacttt atagtggaaa atcagaacgt
    17041 ttagccaatt ggttgaaaat gagtccgatg aagcagttta caacattgga tacgcatgat
    17101 ggaattggtg ttgtggacgc tcgtgattta ttaacagatg aagaattgga ttacacctct
    17161 gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct attcaagtgc caattacaat
    17221 aatttagata tttatcaaat taacagcacc tattatagtg cattaggcga taatgaccgt
    17281 agctatctgt tagcacgtgc gattcaagta tttgcgccag ggattccaca aatttattac
    17341 gttgggttat tagcaggaaa aaatgatatt gaactgttgg aagcaacgaa agaagggcgt
    17401 aatattaatc gtcattacta tgatttagcc gaaattgaag aagaagtgca acggccagtg
    17461 attcaacaat tatttgcact tttaaaattt agaaatgaat ctgcggcgtt tgatttggat
    17521 ggaacgattg aagtagcaac gccatcagaa tcagaaatag tgattacacg taaaaataaa
    17581 aacggtagtg atagtgcaac attaacggct aacttggata cgaaagaatt tatcattgag
    17641 tctaaagata taaaaatttt cactagtgtt gcattaaaat tttaacctat tccatagaac
    17701 aaaaaatcct ttatactagc acttggatga cttatccaag accaataaaa aggactcata
    17761 catggataag tataaaacaa atacagcatt taataaatgg ttttctgcca taaaattagg
    17821 aaaactttct gtcaactccc aaaaatgtat ttttgattac aattcgtatc gtaaaaaact
    17881 gtcttttgaa gctgtgctca agctctttct ctacgcagtt aatgaagaaa aagaaagttt
    17941 gcgtgatttg agtacagctc ttatcgaccg atcgctccaa ctagaaaccg atgtgtcatc
    18001 gattagtcat acccaattgt ctcgagcatt taatgcactt gaacctaaag tgttagaaga
    18061 aatttttcaa caactgttag aaaaagttca acatcaaatc aaaccaacca agcgaaatca
    18121 tctgtatctg attgattcca gtacgttttc gcttagttta aaacgtcata agtgggcgac
    18181 tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac tattgttata tggacgatac
    18241 tacgatgtat ccaactgatt ttaccttaac aaatgcccat gaacatgatc tcaatcaact
    18301 accagtattg gtgaatcaac cagaggcaac ttacgtgttt gatcgtgggt attaggactt
    18361 tgaaaagatg gatcagatgc attgggatgg ctatttcttc gtcactcgga ttaaaaaaaa
    18421 caccaaagtt cacgtgatag atactctgga aacatcgcca gaaacagaaa ttctacgaga
    18481 cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc aatttccgac tagtcactgt
    18541 tcaagataag aacggacgtg tgtttcaatt tatcaccaat cgaatggacg tctcttcaaa
    18601 agaaatctct gatatgtatc acgctcgttg gcaaatcgaa ttgtttttca agcacatcaa
    18661 acaacacatg accattaaaa ccttcttttc tcaaagtgaa aaaggcgtgc agaatcaatt
    18721 gattttaacg atgatttccg ctctcctcac gtttctcatc aaattggaga cgaaaacgga
    18781 aaaatcagtg ttccaaatca aacgattctt tcgttattta ctgtttcagc cctttgaatg
    18841 ttgtctagaa aaattaattc ccacatgaga aaaaagaaat ttggctcgtt acttttggtt
    18901 ttcagttgaa taacagataa aaaattttct ggaaaaagtc atcctctgcg tcagcacagt
    18961 tttttttgac ttattttcag aggaaatagt tttcagaata ttctaaaatg ttttatgcaa
    19021 cactagtgat attttttaga attagatttc taatgtgcat tttttgagta ttaaggagca
    19081 agtatacaaa gatttataaa gctgattaaa tttgaataaa aatatgcagg aacgctacta
    19141 tttttagtag tgttcttttt tatatttgaa taaaaaatgt ggtggttaaa tggtggttat
    19201 aacaaaaaat gaattaatga aattaggtta tgggaagtat cagtctgaga gaatcatacg
    19261 acaggcaaaa gcaattatgg tttcaaaaag ctatacctat tacgaaaata gaagattagg
    19321 actagtacct gttgaatctg tagaagaagt tttaggtatg aagatagagg tagaggagga
    19381 ttttcagaat catggttaat tttaaaaaag ataaaaatgg gaaatatcat tttactgcta
    19441 gtttgggatt cgacaaacga acagggaaaa gagtacaaaa aaacgtagag gggttaaaac
    19501 agttaaagaa gaaaaaaagc gtataccgaa cttgtttcta attatggaaa agaagcagaa
    19561 acttctcaaa gtactatgtt gtttgaggag tttttttata aattattcct accttattat
    19621 caagggaaag tagaagaaag aacatataaa aatcggttat ctacaatcaa aaaaactttt
    19681 cttatttctt taaaatggag ttaagagaaa tacgtccaat tgacattcaa aagtggcaaa
    19741 ataatttatt gagcaaatat aaaaattctt atgtaagaaa catgtatggg atttttcaga
    19801 tgtcattaga tcgagcagtt gttttgggac tacttgaaaa aaatcctgca aaagttattg
    19861 gaaatgtgaa gaaaaggaaa actacagttg atttttggac aaaagaagaa tttacaaaag
    19921 ttatttcaac tttttatatt gatgatttgt accaacattt tggttttatt tgtatgtggt
    19981 tactttttat gaccgttatg cgtttgggag aagccactgc attaacttgg aaagatattg
    20041 attttgaaaa taaaaacttg cgtgtgaata aaacactcta ttataagaga gccaaaacct
    20101 atgagtttgt agaacccgaa acggcttcta gtattcgtac aatcgaacta gacgacaata
    20161 caattacctt tttaaaagct tggaaggagc gtcaagtttc aataggaggc atgtcgtttg
    20221 tactttctta taatggaaca cctactcaga aatatacaat ccgtcatatt atgagaagac
    20281 atgcaacgat cgttggtgtt catataatcc gcatacacgg attaaggcat agtcatgctt
    20341 ctttgcttat atcacaataa aaagatgcac ttcgaattaa agaaaggtta aaacatgctg
    20401 atgtacaaac gactttagga acttatggtc atttgtatct aaattcattg agagagataa
    20461 caaatgatcc cacagattat atccaaacta atttttctaa aatcaaaccg acacctattt
    20521 acgataataa aaaataaatg ataaacaaaa gtaggatcac accaaaagaa acacactaga
    20581 aaaactataa tacaggcttt tctagcgtgt tgtttcttat ttccgcctat ttatacatta
    20641 tactcataaa gcatttaatg catcgatcgt agccgcattg tctatatggg tatataaagt
    20701 tgttgtattg gtacttgaat gtcccaattg ttgggcagtt aataccaatg aatttgtctg
    20761 ttggtagagt ctagtagcta atgtatgacg cagtttgtga gggctgactt gaattctaaa
    20821 gccttttgaa tattttgcta ccatccgttc gattgcaata ccggatatac gcttggcttc
    20881 tccttggtaa attgctagaa ataaagcttt ttcgttttct gttactttat atcgttcttt
    20941 ccgaatagct agataattag ctaaatattc cattgcgaat gctgctatat tgacagcatc
    21001 tcttttattt ccttttctag ttactaccac attattctta tctaaagata ggtcatctaa
    21061 attaatattt ataacctcgg agagtctcaa tccagatcct aaaatcagtg cattgatcgc
    21121 caggtctctt tctacatctc gttgataata gatcaaagct cgtttactca aagtattttt
    21181 atattcattg gcaatataat ctagataatt ttgtgtatcc ttatctagaa ataatttttt
    21241 ctcaatggca ttggcacgtg cagctaacgt agttttatct gtgatggttg ctacttgatc
    21301 cataggatta ttgtctaagt atggttttcc tagaggatta ttggaacttt tcgaaagata
    21361 tttaaataga acctttaatg ccgtgatatt tctattgata gtagaagtag atagacctaa
    21421 gttattatta tctttgagct tagtttttgt tgtctctttt tttacatttt cttttgtgcg
    21481 acgtttcaaa tgagatttgt acaagtttag gtcataggca gttaactgtc ttatcgaggt
    21541 gatagaagta tcaggaacga tagactcact cagaagccat tcataaaaga gcttataatt
    21601 ttttagatat tctaaagtgg ttcgaggtga ttgatcttta tcctcacgat cttgaacaaa
    21661 atcttttata taatcgggca tatgaagtat gattgtatct attttttgat atatttctgt
    21721 atttttcatg tcattcatcc tttcaataag taggggagaa gagtatctct ataattggac
    21781 tataccaatt atagaatgtt ttgtcaattg gtatgctcta aaatttatta atttgtttca
    21841 aaaaaagcat ataaacaggt atacgtttaa taatttaaaa taaatggtat acataaatag
    21901 ggaaggaaat agaagggagc aatctaaatt ttttgaattt tcttaattaa ttatatagaa
    21961 agaacaatct ttctatagag ttagacatga gagggactat gcttgattta aaatcaacaa
    22021 gcgtatatac tgtatataca aagaaaaagg agggagaaga ctatggcaat taaagaaaga
    22081 aaatttaggg aagtggggaa ctcagtagta ttaactattt caaaggagtt tcttaaagaa
    22141 aatgggttaa atcccggaga tacggttttt ttagatgaag aaaaacttcg agcggcaatt
    22201 acaaaaaaac aagccttatc tgatgaaatt gatttagcaa tatctcaagc attaactgaa
    22261 tatgatacta cttttagaga gcttgtcgat aaatgagtat tcattaccta gaagccaaag
    22321 atattataaa aatgaatgta atccaaataa aaaaatactc tcctaatgaa ccccttggtg
    22381 taaaggatgc gaatgcttta gaaatgtgtg tagggcaact tcgagcctca gcttttggtg
    22441 aagaggttta tccttcgatt tatgaaaaag cagctatcct acttatacaa ttgataaaga
    22501 aacatccttt tcacaatgca aataaacgta ctgctttctt agctactttt gtctttttga
    22561 aaatcaatgg acagctttta gctattgatc aaaaagatgc tgtaaattta gcggtatata
    22621 ttgctacgta tgataaggat ttcgatcaac taaaatatga agtgatcaac actataaaga
    22681 cacatacgca gcccatttaa ggagttactg tatgtgtctt tatagggtta ttagctgcta
    22741 tagcgaaata attgaagtaa attacaaata gctataattc aaatccaact atatagaaaa
    22801 cgtaaatttt ctatatagtt ggattaagat aacgactatt tattttatca tatttttact
    22861 ataccctata cttttacttg gtatatttat gaataatcgc aaatttaccc ctatactact
    22921 agtttgtaag ttagattaat aaaaatacta aagttatttg ttcttttatt aaatacacct
    22981 acaacttata aattgatggt atttagataa cacgaaagaa taaaaataac agctgatagt
    23041 aaagtttgtt gacgattgct actcaatcga aagtgagtta gtgcgtatga acttgcttct
    23101 gaaaacttat gaaatgaaag accttttaca tcttgcagaa atcgttactg ggattaaact
    23161 aagcaataaa aactaatgtt cccctcattc tttgaacttc tactatttga tttatttatt
    23221 ctgataaatg acgatttcac cttcaggatc ttcaaatccc gtatagaatt tatttaagat
    23281 ctcttcagta tcagtaatag gataggcgat aggccacata ccctttgatg gttagaataa
    23341 actgctcaaa atctgtatcg tatgttggct ttagcatatt aacaaaggct atctccaaat
    23401 tataaaccat aagcgttgaa tcgttcttaa caatttcaat tcttcctaac ccaaagtcag
    23461 aagatataat taccggactt gccttaccct tcatgtgggt tgttgagatt ccatatttaa
    23521 agggaattgt cggtactatc ggcggtctga aaaacatccc aaaaataaaa aaatctttcc
    23581 cctgctagtc tcctaagtcc ctttgtagcg gatatggcac cataatctca ttttatcgaa
    23641 gtgagtcatg gtttatctgt tggccaacca aaattgatac aatctaacga tgcaccctct
    23701 acggcattct aggggagtgc acttgcattc acccaataaa aaccggcagt tagcaaagaa
    23761 cttatgtagt cgtatgttct tactgttaac atacaagagg tctcctttcc acaattgctt
    23821 agcagtaaac gggaatttac aaagctaatc tcttccattt tcttatcttt gttctaaaag
    23881 tcccaaatgg agcaactgta tttacatgaa taaatttata tacttcccat acagccgttt
    23941 tggttgcctc gtctgcccat tttcttttgt gtgcagtgaa tagatcttca tcactcattg
    24001 aatcaatcat catgttaata tcatcaatat tacgattgag aatttcctct aattctttca
    24061 atggtagatg tgcataggta tttgtaaacc acttatataa ctctcctaac tgattccatt
    24121 taaattgatc agaaggcgtt ttaacagata atccatttct ttcatcattt tcccatttta
    24181 gaattaaatt cgtccaacca acttgatatg ctaaattttc agcaggtgtt ctatccactt
    24241 cctcaactct tttatccttc aattcttcag gaatagtttc aaattctaaa atatatttag
    24301 tataagtcct atttatttct ttttttaatt cttctttatt tttatatgtt ctcattatat
    24361 tacctccata aattcctaat tgtctccttt aattcatcta taatttcatc cacgatattt
    24421 taatccgttc tacaatgcat atctgatatg cattgactgt ctaactcata tgtgaatgat
    24481 ttataaaata tcccaacgtc tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa
    24541 atacgaatat atttcttttg tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa
    24601 atatcaaaat catgttaact actatcaatt actaataaaa actaccactt taagcgttaa
    24661 gaactgttaa gtttagttaa gtgaggatag ttcacaaaat ccattgttaa acttcacgaa
    24721 agtggtaaaa ctcaaaatgc tttatatagg attacagcat cgctctttct tcgattgcgc
    24781 gttgggtaaa aaatattatg aagtaaaaat tgacgatgac acgttcctta cggctcatca
    24841 gatgaaacag ctacaaaaac gaaatgatca actcgaagaa aatttaattc taaaaaagcg
    24901 attgccatat tcacgcctcg ctcgacgaac tattaaaggt caagatttac ctgaagatca
    24961 tcctttttag gaaattgaag ctttttatgt tgcacaatgc atttataata ctacaatgat
    25021 gttttcacca gatattcttc tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa
    25081 aattcaccgc gaatttacta aaattatgaa taattatgtt aatcttccga aatttttttc
    25141 caaaattaag ggatcaagct ggtgttatag attgtttaag gttggcaaaa aaataacttc
    25201 tttttaaaca attatagaaa accgatactc tgttaagttg gttgacacgg tgtcgatttt
    25261 tatgactttc ttattctgaa acttcactaa cggacagtac ctaaaaagtc tatagtatgg
    25321 acgtttacga gtactatttt atcaaaaatg ctgctcattt caacttatat gttttagtga
    25381 actaccccca cttagcttgg ataggctaca attaactaga cagaaaaatt aaggtgtgta
    25441 gactagaaga aaacatacca ggaggaattt ttatgtctaa gagaacacga agaacttttt
    25501 cacaagaatt caagcaacaa atcgtcaatc tttacttagc tggaaagcca cgtgtagaaa
    25561 tcattcgaga atatgaacta acggcttcag catttgacaa atgggtaaag caatc
//
LOCUS       DASJTM010000038        25174 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJTM010000038 DASJTM010000000
VERSION     DASJTM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.038"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..1877
                     /locus_tag="U4B29_001442"
     CDS             237..1877
                     /locus_tag="U4B29_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN2106395.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="U4B29_001443"
     CDS             2031..3353
                     /locus_tag="U4B29_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN2106396.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="U4B29_001444"
     CDS             complement(3415..3846)
                     /locus_tag="U4B29_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106397.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="U4B29_001445"
     CDS             complement(3910..4746)
                     /locus_tag="U4B29_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN2106398.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="U4B29_001446"
     CDS             complement(4936..5238)
                     /locus_tag="U4B29_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN2106399.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U4B29_001447"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U4B29_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN2106400.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="U4B29_001448"
     CDS             complement(5692..5958)
                     /locus_tag="U4B29_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN2106401.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="U4B29_001449"
     CDS             complement(6212..6937)
                     /locus_tag="U4B29_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2106402.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="U4B29_001450"
     tRNA            complement(7303..7373)
                     /locus_tag="U4B29_001450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="U4B29_001451"
     CDS             7490..8242
                     /locus_tag="U4B29_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2106403.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="U4B29_001452"
     CDS             8258..8737
                     /locus_tag="U4B29_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2106404.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="U4B29_001453"
     CDS             complement(8988..10568)
                     /locus_tag="U4B29_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN2106405.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="U4B29_001454"
     CDS             complement(10561..11304)
                     /locus_tag="U4B29_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106406.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="U4B29_001455"
     CDS             complement(11480..12256)
                     /locus_tag="U4B29_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2106407.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="U4B29_001456"
     CDS             complement(12504..13340)
                     /locus_tag="U4B29_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN2106408.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="U4B29_001457"
     CDS             complement(13351..14280)
                     /locus_tag="U4B29_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN2106409.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="U4B29_001458"
     CDS             14472..14825
                     /locus_tag="U4B29_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2106410.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="U4B29_001459"
     CDS             complement(14899..15555)
                     /locus_tag="U4B29_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2106411.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="U4B29_001460"
     CDS             15781..17715
                     /locus_tag="U4B29_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2106412.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="U4B29_001461"
     CDS             complement(17801..19024)
                     /locus_tag="U4B29_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2106413.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="U4B29_001462"
     CDS             complement(19018..20916)
                     /locus_tag="U4B29_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN2106414.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="U4B29_001463"
     CDS             complement(20910..22028)
                     /locus_tag="U4B29_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2106415.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="U4B29_001464"
     CDS             complement(22111..22944)
                     /locus_tag="U4B29_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2106416.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="U4B29_001465"
     CDS             complement(22937..23854)
                     /locus_tag="U4B29_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2106417.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..>25174)
                     /locus_tag="U4B29_001466"
     CDS             complement(24081..>25174)
                     /locus_tag="U4B29_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2106418.1"
                     /translation="LNITLLFSLSACGKTNEEVTANIADPMIKKATTLEVYVANGKLT
                     NYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPDIISMADTSAAG
                     VLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWASDFTPALCYYQP
                     KKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFINMLAQQNQSLL
                     DEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMFVAGGWYATNMS
                     LNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQQFLTFMLTDEE
                     CLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7763 a         5023 c         4296 g         8092 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt ggctctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agca
//
LOCUS       DASJTM010000039        24338 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJTM010000039 DASJTM010000000
VERSION     DASJTM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.039"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..2478)
                     /locus_tag="U4B29_001467"
     CDS             complement(133..2478)
                     /locus_tag="U4B29_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2106419.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="U4B29_001468"
     CDS             2757..3659
                     /locus_tag="U4B29_001468"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN2106420.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="U4B29_001469"
     CDS             3870..5645
                     /locus_tag="U4B29_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN2106421.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="U4B29_001470"
     CDS             complement(5711..7297)
                     /locus_tag="U4B29_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN2106422.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="U4B29_001471"
     CDS             7548..7772
                     /locus_tag="U4B29_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN2106423.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="U4B29_001472"
     CDS             7914..9665
                     /locus_tag="U4B29_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2106424.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="U4B29_001473"
     CDS             9665..11449
                     /locus_tag="U4B29_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106425.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /locus_tag="U4B29_001474"
                     /pseudo
     CDS             11784..12636
                     /locus_tag="U4B29_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12733..14619
                     /locus_tag="U4B29_001475"
     CDS             12733..14619
                     /locus_tag="U4B29_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2106426.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="U4B29_001476"
     CDS             complement(14660..15562)
                     /locus_tag="U4B29_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2106427.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="U4B29_001477"
     CDS             15792..17195
                     /locus_tag="U4B29_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2106428.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17192..18628
                     /locus_tag="U4B29_001478"
     CDS             17192..18628
                     /locus_tag="U4B29_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2106429.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="U4B29_001479"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="U4B29_001479"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN2106430.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="U4B29_001480"
     CDS             19912..21639
                     /locus_tag="U4B29_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN2106431.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="U4B29_001481"
     CDS             21655..22005
                     /locus_tag="U4B29_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2106432.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="U4B29_001482"
     CDS             complement(22099..22959)
                     /locus_tag="U4B29_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN2106433.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="U4B29_001483"
     CDS             23051..23479
                     /locus_tag="U4B29_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2106434.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /locus_tag="U4B29_001484"
     CDS             23641..23817
                     /locus_tag="U4B29_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN2106435.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="U4B29_001485"
     CDS             23844..24290
                     /locus_tag="U4B29_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN2106436.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5187 g         7090 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaattc
//
LOCUS       DASJTM010000040        24076 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJTM010000040 DASJTM010000000
VERSION     DASJTM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.040"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            150..563
                     /locus_tag="U4B29_001486"
     CDS             150..563
                     /locus_tag="U4B29_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2106437.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1183..1347
                     /locus_tag="U4B29_001487"
     CDS             1183..1347
                     /locus_tag="U4B29_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106438.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            1344..1574
                     /locus_tag="U4B29_001488"
     CDS             1344..1574
                     /locus_tag="U4B29_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106439.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            1759..2304
                     /locus_tag="U4B29_001489"
     CDS             1759..2304
                     /locus_tag="U4B29_001489"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2106440.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2305..2622
                     /locus_tag="U4B29_001490"
     CDS             2305..2622
                     /locus_tag="U4B29_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2106441.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2779..2943)
                     /locus_tag="U4B29_001491"
     CDS             complement(2779..2943)
                     /locus_tag="U4B29_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEN2106442.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3045..3566
                     /locus_tag="U4B29_001492"
     CDS             3045..3566
                     /locus_tag="U4B29_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN2106443.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3553..4026
                     /locus_tag="U4B29_001493"
     CDS             3553..4026
                     /locus_tag="U4B29_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2106444.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(4033..4191)
                     /locus_tag="U4B29_001494"
     CDS             complement(4033..4191)
                     /locus_tag="U4B29_001494"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEN2106445.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4284..5045
                     /locus_tag="U4B29_001495"
     CDS             4284..5045
                     /locus_tag="U4B29_001495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2106446.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5265..6584
                     /locus_tag="U4B29_001496"
     CDS             5265..6584
                     /locus_tag="U4B29_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2106447.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            6600..7820
                     /locus_tag="U4B29_001497"
     CDS             6600..7820
                     /locus_tag="U4B29_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2106448.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7855..8532
                     /locus_tag="U4B29_001498"
     CDS             7855..8532
                     /locus_tag="U4B29_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2106449.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8529..9722
                     /locus_tag="U4B29_001499"
     CDS             8529..9722
                     /locus_tag="U4B29_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2106450.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9737..10063
                     /locus_tag="U4B29_001500"
     CDS             9737..10063
                     /locus_tag="U4B29_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2106451.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10067..10402
                     /locus_tag="U4B29_001501"
     CDS             10067..10402
                     /locus_tag="U4B29_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2106452.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10399..10794
                     /locus_tag="U4B29_001502"
     CDS             10399..10794
                     /locus_tag="U4B29_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106453.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10791..11162
                     /locus_tag="U4B29_001503"
     CDS             10791..11162
                     /locus_tag="U4B29_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106454.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11178..11738
                     /locus_tag="U4B29_001504"
     CDS             11178..11738
                     /locus_tag="U4B29_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106455.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11863..12174
                     /locus_tag="U4B29_001505"
     CDS             11863..12174
                     /locus_tag="U4B29_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106456.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12198..12389
                     /locus_tag="U4B29_001506"
     CDS             12198..12389
                     /locus_tag="U4B29_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106457.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12389..16237
                     /locus_tag="U4B29_001507"
     CDS             12389..16237
                     /locus_tag="U4B29_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN2106458.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            16238..17110
                     /locus_tag="U4B29_001508"
     CDS             16238..17110
                     /locus_tag="U4B29_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN2106459.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            17107..18156
                     /locus_tag="U4B29_001509"
     CDS             17107..18156
                     /locus_tag="U4B29_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106460.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18160..18984
                     /locus_tag="U4B29_001510"
     CDS             18160..18984
                     /locus_tag="U4B29_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106461.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDIQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            19002..21062
                     /locus_tag="U4B29_001511"
     CDS             19002..21062
                     /locus_tag="U4B29_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN2106462.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            21079..21228
                     /locus_tag="U4B29_001512"
     CDS             21079..21228
                     /locus_tag="U4B29_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106463.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            21225..21671
                     /locus_tag="U4B29_001513"
     CDS             21225..21671
                     /locus_tag="U4B29_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106464.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21673..21810
                     /locus_tag="U4B29_001514"
     CDS             21673..21810
                     /locus_tag="U4B29_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2106465.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21845..22087
                     /locus_tag="U4B29_001515"
     CDS             21845..22087
                     /locus_tag="U4B29_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEN2106466.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22103..22300
                     /locus_tag="U4B29_001516"
     CDS             22103..22300
                     /locus_tag="U4B29_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2106467.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22311..23327
                     /locus_tag="U4B29_001517"
     CDS             22311..23327
                     /locus_tag="U4B29_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2106468.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            23442..>24076
                     /locus_tag="U4B29_001518"
     CDS             23442..>24076
                     /locus_tag="U4B29_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106469.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSS"
BASE COUNT         8373 a         4052 c         5322 g         6329 t
ORIGIN      
        1 aaagataaaa aaataagtaa ggcttctcat taaaatataa aaaagacagc cgaccactga
       61 ctgcctatat aagagtattg aaataaaaag ctgctgatat aataaattcc acaagtttat
      121 tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac
      181 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag
      241 gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag gcgccgaagc
      301 acggcaataa ggcagaagac gcaatcattc agatgatgga tatagaagcg gagagagacg
      361 cgattctagc agccttgatg gctcttagtc tgattagtcg tcagatactc tactatagct
      421 tttgtgtgcc agatagcttc tcaaactaca gaattagccg tgaagtgggt tattcagaaa
      481 gaagtataca acggatgaag tcggaagctc taatagagtt tgcagaagca tataaacacg
      541 gaagaataat tgcttataaa taatttggcg gctttttggc ggaatgatgg cggtttttag
      601 ctatttacca gtgatattat ggtagtgtcg aaagattagt gataggtcta agacaaaata
      661 ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata gacagcaacg
      721 gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt attatctatc
      781 atccgttgct gtctattgtt tttttaatta ttcacacgat aactaaaggt gagcgaagag
      841 aaatgattcc attaataatt tcaatttttg cgctttgtct taatgtctat atgattggat
      901 ttaaaaatgg gcaaaataaa agatagtagt aactaagaaa gaaattttta tattgtcact
      961 gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc aactatgcaa
     1021 tgttcgattc attgccagtg actttggcaa ccgaggcatc ggcggtttaa aaatataggg
     1081 gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata ttagatcact
     1141 cgttgagtgg tctttttatt ttacataaag gaggtaacaa cgatgtatag accacaatac
     1201 ttagaacaga agtatgaagt aatcactgtg caaaacggta acggtgagat agtacgaaag
     1261 tatagaagac caataaagag cgatacatat aaacgaaagg aaaacaatga agttatttct
     1321 tttcgcagaa ggagaaaagc caaatgagaa actactggta tgtatcgcta actaatgaat
     1381 atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga
     1441 agtactctat tgtcgaaatg ctaagagaag ctacaccaaa cgaagtggat aaatgcaagc
     1501 tgatatattg cggtcatggc tattggaaag acgagtatat ccaacaaaac attgagaggt
     1561 ggatagatag atgagttacc tcgaacattt gaaacgttgc tacatgcatt ctaagaataa
     1621 acttcctgac agctacactg tagatgatgt tgctatacat gtattgaaga ctgagagcca
     1681 tagcagtcct gatggtagca gtaaggagca gacgctcgcg tggttcaagt tctttaagtg
     1741 gattaaggag gaagagtaat gcacatgcac ataggagaaa agatatataa attgagacgg
     1801 cttaataacg aaacaatgaa cgacttagtt aaacaaactg gagtgtcaaa atcatggtta
     1861 tctgatattg agtctggaaa aaagcaacgc gttgatttta ataagatatg tgcaatagcc
     1921 aaacattata aagttagctt agaatacttt cgggatgatt atttggatag tgatgaacac
     1981 gaggatatta ttcctttgac aggcgaaaca agagttaaat cagtgaaatc gcttgaacaa
     2041 agcctaaagt atctgaataa caaacaagaa cctaaccttc tagagatcaa actaaaagat
     2101 actgactcag taccagaggt ttggtacaag ggtgagaggt tggatgaatt gcctaaagga
     2161 ttagtagatg tctcgtatca ttggaagact gatgatttta ctaatgatga tagaggagcg
     2221 aacgacatca cgattcaata cttttctagc tttaatgata agtatccaga taagaaaaca
     2281 atcggacaca agagagatat gtaaatgaaa gaagttagac ctagagacga aatagacaaa
     2341 ctatacaaga ccaaacgatg gcgagaccta aggcaagtag taatagctag ggacttcggc
     2401 atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg gcacgatcat ccatcacata
     2461 gtcgaggcga gggaagacct gtcactgttc tggtccgtag ataaccttga atgtatctgt
     2521 gtagcttgtc acaacagaga gcatccagag agatcaggcg ggaagaagaa accaaaacct
     2581 aaatcacata tcgttaaaat gtattcaact cctgaaagat aagtttgcag cgaaatgaag
     2641 gtagcccccc tactctaaaa gattaaagag taaggcttga gaagaacggt gctgtccttc
     2701 cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa ggaaaaagcc gaccaattta
     2761 agccggcttt ggacgaagct atttcttagt ccatttgttt cctttttgag aagtaggggg
     2821 taatcggtcg cctggatcaa tttttacttc ttgtccgcct tggacatttc caccacgagg
     2881 acccacttct ttgtaggttc cttttggttt gttgtcttcg ccgggtttat agagttctcc
     2941 cataggaatc cctccttaac aaatttcggc acagcactgc cgataaccta attataagga
     3001 ttgtgataac gatttcaatc tatcttttga aagaaggtga tattatgccg caaccagcga
     3061 agagtgcaaa attacaatta ttaaacggaa acccaaataa gaagaatacc gaagaactcc
     3121 gcaagcgagc ggccgcagaa gacaaattaa aaatggctac tgacaaaatc aaaccgccgt
     3181 tatggctaga ttcgctagga aaggatacct ttgagtttat cgccgatgaa ttgctgtctg
     3241 tggatttaat cagtaatccg gacgtccata caatggctct atactccaat tggtattcgc
     3301 aatacgtttc tttagaaaaa cagcttcgaa aactacaacg agagtacaag ttgaactatg
     3361 cgcttgcgaa aaaggaggca gaggcgagag gtgagccgtt taatgaacct aatgaattaa
     3421 ttggtaatcc gctctctcgg cagatggata cagcgtctcg gaatctccgt tcttttggcg
     3481 ctgatttagg actatcacca gcagccagag ctaagttagc tattaagatg gctgatgatg
     3541 gtggtgatga cgatgacgac ttctaatatt ttggatatgt cttacacaga acgtgtggac
     3601 tattggcaaa gctatcttga ggagcaagct tcttggggtg gctttttaaa atgtccatat
     3661 ccggaattgt taactacttg gtatgcggaa cgattaatcg atggaagcat accagccagc
     3721 aaagaaaata ttcaagctgc taaacggcat atgcgtgatt tgcagcgcca aggaacagat
     3781 gattttcctt ggatctttga cgaagaaaaa ggtcaccggc ctattagata tatcgaaaaa
     3841 aaatgtaaac caactgaagg cgactttggt tcgtttgttt tgcaaccttg gcagcatttc
     3901 ataattggat ccatgtacgg atgggtacat cgtgatacag gagagcgtcg cttccgcgag
     3961 gctcttattt ttgttggacg taaaaacggt aagttttgcc gtttaaaaat cgggcaaaat
     4021 cggtgaaaac ctttattttt ggctttcttt tagtaataat tcgatagctt tatcaagcaa
     4081 tttagacata ggaaccatag tttcttccga catttttttt aatccttcgt ataaatcttt
     4141 atcaattgcg ttagaaattc taattctgtt ttttagtccg tatttgtcca tgtgtgacac
     4201 tcctttttat tttactataa catccgattc caccgcttgc aactaccatc aattgatggt
     4261 agtatataaa agaggtgagt agattgaaaa aatacattat ttacaaacac ttaaataaaa
     4321 taaatggcaa aatttatatc ggtgtcacaa atgatattgg cagaaggtgg aggagcggtg
     4381 ggattgaata taaaccacca aagaacgaaa cccaacacca cagaagtttt tggaatgcaa
     4441 tacaaaaata cgggtgggaa aattttgatc atttgattat tgaagaagac ttaacgatga
     4501 aagaagcttt tgagaaagaa aagttttata ttgaacttta tgactcaact aataagaaaa
     4561 aaggttataa catcgcaaaa ggcggaaatg gcggaataat atataaagtt caccccaaag
     4621 gaatgctagg gaaaaaacaa tctaaagaat tttcttcaaa tcacagtaaa tgggcaaaga
     4681 accataagaa taattgtatg acaaacggtg atgttgtatg gggtgttact cacgaacacc
     4741 caaaaggcat gttgggaaag caccacagca aagaatcaat tatgaaaaag aaagcatata
     4801 gtggtgaaaa tgctgtaacc tcaaaaccaa ttgtcgcaat tgaacgggat ggaactaaaa
     4861 gagaatttca cagcgctaag ctttgcatgg cttactattc gattagtaca tgtgtttttt
     4921 accgactttt aaaagatggc gccccttatg tgataaatcc taaagcgaac tacagaaata
     4981 aagaaaaaat attagctatc gaaggaatta tgttcaaaca taaagaagat accgaggtaa
     5041 gttagtagat tgcgaaaggt tactaactac cgtaacgcgt aggagttgaa taaatataat
     5101 gctcccaaga gtgtccgaca gcagataatt tgctgaaaat gtacgctaaa ctgggccaga
     5161 actgactgac cgatgaaaat gagggtgacc tccagagtgc gagataaaaa gctcgcagat
     5221 aataacaatc gaaaacatca ctaatttcag gcctttccac atacatggtc gcttatgatg
     5281 atgaacaagg cgccaacgtt tacgtattgg caaatgctcg tgatcaagca agcttgttgt
     5341 ttgataaggc cgcagaaatg gtcaaacaat cgccggcgct ctttaagaaa tttggtaagc
     5401 ctaaacgatc aagtattaat tatgctcccg ccttttctaa aatggaacca cgcgcctcag
     5461 atagccggaa attggatggg ctaaacactc actttggtat ttttgacgag atccacgagt
     5521 ttacgaatta caatctgatc aacgttatca agaaatcaag aggaaccaga aaacagcctc
     5581 tgatagttta tatcacaact gctggatatg tattagatgg tccgttgatg tcttattttg
     5641 agcaaggtgt ggactgtttg gaacatttgg aagatgacat cgatgaacgg actttctatt
     5701 atctggcaaa acttgacagt gcggaagagg ctgatgaccc aagattatgg atcaaagcca
     5761 atccgaatat ttgtctaatg aattttgttg gcatgctaga tgactatgtt aaggataaaa
     5821 aagatccaaa agaatatgct gactggatta ccaagcaatt taacttgttt tccgatatcg
     5881 atgagctgtc atttgtcgat atgcctacca ttaaacgaaa caataaaacc atcgatattg
     5941 aaacgctcaa aggtaagaag tgtgtcggtg gttttgactt gtccgaaacg gaagacttta
     6001 ccgcagccgt tttagaattt ccgcttgaaa caggcgaggt attcattttg caacacacat
     6061 ggatcccaca agctagattt gatcgagata acaatcaaga gcgtatcaaa gcgtgggaga
     6121 aggtgggaga tctaacgatt attcctggtg attacgtcaa ttatgaatac gtcttaaatt
     6181 ggtttgtaga aaattcgaaa atctatgaca ttgtaaaaat caattatgac aaggccaagg
     6241 cgctacgatt aaataaagaa ctagaaaatg caggatttga aaccaacgag attcggcaag
     6301 ggtttctatc attaggtggg ccaatgcaaa acttcaagga aatgctattg gacggtaagg
     6361 tgattttcaa caattccaag ctttaccgat ggtatctatc caacgtcaag ctggtgatgg
     6421 atcgcaactc aaactggatg ccgtctaagc agtccaagag tagaaaaata gatggttttg
     6481 cagcaagttt gaacagccac gccgaagtgt tgaatatgtt ggttaatcct gtcggaaccg
     6541 ggaaagtaac ctattactcg atttccgatt taatgaatat gtaagaaagg tgtggaggaa
     6601 tgagtatttt agatcgtttg cgttcttttg gccgagcgaa gccgaaagcg agcaaacaag
     6661 agtatttttt gaatgacccg ggattgatac cgtatttagt cggaaaagat gaaatatcag
     6721 aagggatttt ttccgtaatt agccgtgtat cgaacgtttt tgcgtctctc cctctcaaaa
     6781 tgatagatgt ggagtttggc caaccggacg actgtcctgc atacaacttg ttgagcgaag
     6841 gccctcgata ttttacaaag tttgattttt tccgggacgt ggaagttttg agaaactacc
     6901 aagggaatgc gtatgtgcag attttccgaa atatcaatgg agaagtagca gatatggcgt
     6961 tagtaaaacc tggtgcttgc catccagtga ttgatatgga tagcggggag ctttactacc
     7021 aagtaactgc gactgacaaa ggcagttaca agcaagttat ctatgtacat tacatggaaa
     7081 tgcttcactt taaacaaccg aggtttggcg gcttggaagg tacagacccc acaaaagtat
     7141 taacgaatac cctcggatat gatcgagaag tccgaaaaat ctctttaagt cagcttaaag
     7201 gaagtaatga agggctaaaa gttaagtttg ctagcaatat ggatgaagaa gctaaaaaag
     7261 ctacagttaa aaacattgct gatttttatc gacaaaacgg tggactactt gtggaagaaa
     7321 acggtgtaga aatcgaacgt ttacaacgag agctggtaga cagcaagctt ttagatactg
     7381 ataaagtatc tcgctccaga atcgctatgg tctacaatgt gccggagcat ttcatcggga
     7441 ataaccagtc gagttactcc tctcaggaac agctcaatat ggaatttttg acgtacaatc
     7501 tagtaccgac cgttaatcaa tatgaagcgg aactaaataa gaaaacacta tcgagaaccg
     7561 aaaaagcaaa aggttatcga tataagttta atgtcgcaag tttgctaaga gctgacactc
     7621 aggcaagggg acaattctat caaatcatgc gacgaggcgg agcatactca gcgaatgatg
     7681 tccgaagatt tgaggacttg caaccgataa ataaaaccgg tatggatgat taccatattt
     7741 ccggagacct atatccaatc gatatggatc caacattaag aaaaacaacc tcgtctaaaa
     7801 gcgtagccga aaacggttag gtttttttag tttgcaccga agggaggtgg aaaaatgaaa
     7861 aaagtgacgt taagcggcga tgtcgtggat aacgataccg cgtggcttta tgactggttt
     7921 gggatcaatt gtatctcacc agggaaaatt tctgccgctc ttacagaagc agcgggggat
     7981 gaagtagaac ttgatatctc atcgaacggt ggggatgtcc tagcggcaag cgaaatatat
     8041 accgctatcc gtgcctatcc gggaaaggta tccggaaatg ttgtgagtat tgcagcaagc
     8101 gctgcaagtg taatcgcttg cgcttgcgaa ccgcttagaa tctcacctac ggcacacatc
     8161 atgattcata acgcatgggt gaccactagt ggcaacgctg aggaattaaa agccaatgca
     8221 gaaatgttaa gcagtgtgga tgagtctatt gttaatgctt acgagatcaa aacaggactc
     8281 gatcggaaaa aacttgctga tttaatggcg aaagatactt ggttaaatgc tcaaacagca
     8341 gtagcagaag ggtttgcaga tgaaattatg tttgcagaag caccagtaac ggtactcaat
     8401 gcctctcaac cggttattcc aaaaaacgca gtaactaagt tgaaaaattt aatactcaaa
     8461 gcggaagcac cgcaagaaga aacactctta cagaaaaaac taaaagcctt aaatggaggg
     8521 aaaaacgaat gaatttagaa caattaaaaa atgcgtgggt cgaggcggga agtaaagtct
     8581 ctgacttaaa tgcacaactc aatgcagcat tggttgacga tgaaaaaaca gaagaagatg
     8641 tagtaagttt gcaagcacaa gtaaaagcag cacgggctaa acgggacgga ttgaaagagc
     8701 aagtggcaaa tatggaagcc gaacaagtct taaacgtcaa aaaagaacca ttagataaaa
     8761 aagatgaaaa cttgaaaaac aagtttatca aagactttaa agcgatggtc aatggtgatc
     8821 ctgctattat ggctactttg acatctaata cggatgaatc tggtaatgct atcggattga
     8881 ctattcctgt agatgtgcaa acgactattc atactttggt tcgtcggttt gactctttgc
     8941 aagaatacgt aaacgttgaa aaagtgacca ctaccagcgg ttctcgcgtt tatgaaaaat
     9001 ggtctgatat tacaccgttg actgctttgg atactgaaga cggtgaaatc ccagcaaatg
     9061 atgatcctgc actttacttg atcaaatact tgatcaaacg ctatgcaggt atttccacag
     9121 taactaacag cttgctaaaa gatactgccg aaaacatttt ggcatggttg tctaaatgga
     9181 tcgcgaaaaa agtagttgtt actcgcaata caaaaatctt ggcagctatt gatggaatca
     9241 aagcggcaca aaagaaagat gttacagatg ttgatggaat taaagatatc gtaaacgtcc
     9301 aacttgatcc agctatcgaa gctacatcta tgtttattac aaaccaagat ggcttcaatg
     9361 ttttagataa agtgaaacgt gctgatggat cttacttgtt acaaaaagac gtagcttctg
     9421 caactggata taccttctta ggtaaaccaa tcaagaaaat tgcttctcgt ttcttgccaa
     9481 acaaaggtac gcaagctact cctaaatatc cactgtacat tggtgatctg aaagaagccg
     9541 ttacattgta tgatcgcgaa aacatgagct tgctgacaac gaatattggt ggtggagctt
     9601 ttgaaacaga caccactaaa gtacgcgtca tcgatcgctt cgatgtgcaa ctagttgatg
     9661 atgaagcggt tgttttggct acttttacaa ctattgcgaa cgagacaccg gcgaaagttt
     9721 aaggagctga ttccttatga ttcttgatcc taaaatggat ttaggcgaaa tcaaaaacgc
     9781 actaaagatt gataccgatg atgacgatgt ggaagtaagc cgtgcggcac aagctgcaat
     9841 tgcatacatt agaggggcta tcgggaatga taagccctct ttttataagc aagaaaatga
     9901 aacggttgat ctgattaatt tagctatcct gcaattagca gatcattatt acaacgctcg
     9961 atcagcaacc gtgagtggga acttgcgaga gtacgattta ggttttacaa gcctaatctt
    10021 gcaactcaaa gcaagttatt tgctttttgt ggaggaggag tagcgtatgc cccttatcca
    10081 aacaggaaat ttaaatcaac gcatcaagtt tgtccgagat acgacggtta aggatgagga
    10141 cgggcaagtt gtcccgactt ctacaaccat tcttacttgc tgggcaagtg tgcagacaca
    10201 acgcctgaac gatattaaga cgtcgattgg tacggctttg gaaggaacac tgacgttcat
    10261 tatccgctac caacaaaaat cagagctgac caatgatatg aaagtgcgtt ggaatggaaa
    10321 aacgtttgaa atcattacga ttacgaaagg cgagtttgcg aaggacttca cgacaatcat
    10381 tgcaaaagag gtttcaaaat gagtgtagaa gtcgatgcaa ccgaagtgta caaagcgctt
    10441 agggaagtaa aagcaaacgt tcaacgagtg gaaagcccag cacttagaaa agctggggag
    10501 tacgctcaag aagagttacg acaaaacaca ccttactggg atggaacgaa gtcaaacggt
    10561 aaacgtggtt cgtatatgca agaacatgct aagaaccatg tggttacaag ctcgataaaa
    10621 aacggattgg tagaagtcgg ctatgacaaa gatgtttctt ggcggatgca ctttatcgag
    10681 ttcggaacaa tcaaacaacg tccaaaaggt ttcgtacaaa aaacacaaaa gcaaatcgaa
    10741 aaacaagtaa cacaaatcat tgctgacgaa gtaaaaagga ggctaggact ttgaaaacgg
    10801 cagtatcaca agtctattca attctgaata gcaatgaaaa aacaaagaac attgattttt
    10861 acaccaatag tgttccggaa tcagctcaaa cagtacctag ccttccagtt ggcagaatta
    10921 cagagatatc cggcaactat gaagattttg caagcaacaa tcctttgacc attcaattta
    10981 acgtacaggt agatgtatgg gtgtcaacca tgaaagaggt tgatgccttt tattttgccc
    11041 ttgatgaggt tatgaggggg aatggttggc aatgcgcata cacggaacaa acagatgacg
    11101 aggacttgga aggtgcaaag cggattatca aacgatatgt agcaaatatt tcactaaatt
    11161 aaaaggagag aaaatagatg gcaacagtag gattcgagag cgtcattttt ggcgtaaaaa
    11221 caggtgcagg cggcactcta aaagaattag tagcagataa gtcgaaaggc ggagcgatcg
    11281 aagctaaaat tactggatta ggcgcaactt ctaacacaac atacgcttca aacgtaccgt
    11341 tcttcattgc aagtaaaggg gtttcgtcgc caaaagttac gcttgacgtg gcagacttaa
    11401 tggataacgg catttacagc gaaatcattg gtgctaaaac cgtggatggt gcaaatgtaa
    11461 ttggttcaga aactgaagcg ccttacgtgt cggtagtcat ggttacagcg aacaaagaag
    11521 gaaaacgctt gttcatggga ttgacaaaag gaaaattcag tcatccagat atcgacatga
    11581 aaacagctga agacaaaggg gtagaattgc aaaccgattc catcgaaggg gaattcattt
    11641 ctgatgaacg tggctatgta tacttaacag ccgtagaatc agaaggaatg accttacaaa
    11701 aattcaagga cttggtaaat aacaaagagg gggagtagtt aaccctgcat ctacaccaac
    11761 gacagataca gggacaccaa aagaaccaga accaaaaatt gatacacaag gttagccatt
    11821 tttggctagc cttatttttt gtaaaaacaa ggaggaaaac aaatgattga attgcaattg
    11881 aaacttgacg gaaagaaaaa aacattcaaa caacaagata tttccgcacg tgcaatgcgt
    11941 gagtgtatca aattttacga gaaagcggaa aaagcagacc taactgattt agaagcgatt
    12001 gattcaatga ttgcaattac agcagatatt ttccaagatc cagcagttac atttgatgct
    12061 attttagacg gtttgactgc gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa
    12121 ataaatgaac tgggaaacaa tgaaaaaaag cagacggcga gcaaaaagaa ataagttttt
    12181 ctgaagctag gaaagcaatg gatcaaatct acaaagattt aatcgaagca ggttggacga
    12241 tgagagatgt ggacgaagcc gactatcatt atttgttaca cctttttgga gaagtggaga
    12301 gtggcgaaga atatgtagat ggtgctgatt tcatcaaaca atttttatcg gctgaagact
    12361 tagtaaaact tgaggaagga ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat
    12421 caagctaggg attagtttag atagcactag ttttggtaac acgctggacg aaatcaatgc
    12481 gaaagtcaaa caagctgagt cgaatatgcg tgccaatcta aaggcttatg attcagcagg
    12541 acgttcatac gaagcactta gtcaaaagac gaaagacttg tctacggtta tggaagggca
    12601 aaacgccaaa gtaagagaat taacaaagcg ccgtgatgaa gcgattagca agtatggcga
    12661 ggaatcgaaa caagttgcta accttaacac acagataaac aatgctaccg caaaatataa
    12721 tgcttacagt cgccagttga acgacacaaa aaaagaattg gtgtattcca aaacagccgt
    12781 caatgattta tctaatgaaa tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc
    12841 gttgaaagcc gctggtgatg aatctggtgc ttttgaagca aaacaaaaag ggctagccaa
    12901 acaaacggaa ttatccgaga aagctatcga agaacagcgc aaagttgtga aactgatggc
    12961 tgatgagttt ggcgattcag caaatgaaac cgaagatgca aaaagggcat tagaaaagtt
    13021 agaacgacaa agccaaatat ctagcaggca attagaagca ctcaaaagct ccagcgatca
    13081 atcaggaaaa gaaatagaag attttggcga caagtccaca aggtcagcta ggaaactgga
    13141 cggactaaaa gacaaattaa gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg
    13201 attagcgcat aacgctatta gcagtgtagt aagtggcgtg caaggtttgg ttggcgaagc
    13261 agtaaacgca tcggattcat tgatgaagtt ttccaaaacc atggagtttg ctaactttgg
    13321 gaagtcacag atagaaagct cgaaaaaaga aatgaaagac tacgccgata agacggttta
    13381 tggtttagaa gaaattctga acacaaccgc acaattggca tctaatggga ttcctaacta
    13441 tacagaacta accaaggcgg caggtaactt gaatgccgtt gcaggcggtt ctagtgatac
    13501 attcaaatcc gttgccatga tgctaacgca gacggcagga gctgggaaac taacaactga
    13561 aaactggaat caattagcag atgcgatacc gggtgcttca ggattgttac aagacgctat
    13621 gttgaaaaac ggagcttata caggaaactt ccgtgatgca atggcgcaag gtcaaatcac
    13681 ttccgacgag ttcaaccaag caattgtaca gttaggtatg aatgacggag cagttaaggc
    13741 agccacttct acagacacat tgagcggttc ttgggagcag atgaaatcca ctgtaataaa
    13801 tgggctacaa agtattatag aaaaaatagg cgttgaaaat atcactggtt tcatcaacag
    13861 agtaacaaaa gggattgaaa attctattcc taaaattact caatttatag gttggttgag
    13921 agatattgga acgtggatcg ttgaaaatag agagccacta acatggattg tcggaatcat
    13981 aggcggaatt acattagcag taaaagcatt gaacgtagca agtatgttgc tggcaattac
    14041 tggcggaaca ttggcagccc cttttgtggc gattggtgta gcattaggcg cactagcagg
    14101 tgctttggtg atagcttata caaaatctga aacatttaga aatatagtca atgcggcttt
    14161 tacagctgtg aaaaacgtag ttatgagcgt tatcaataat ttggtggaat actacaaaat
    14221 gttgtggagc gtgttgcagt ggctttggga aaaaataaaa gaatgggctt catggattgg
    14281 caataaattc attgaaatga agaacagcgt tgtgaatacg gttcagaacc tatggaatgg
    14341 cgtgaaaaac ttcttcagca atggcgttgg agatacgtgg aataaggtag tcggttgggt
    14401 aaaaaacatt ttcaacaaag caactgaatt gaagaacaac gtttctgatg taatcggtaa
    14461 cctgtggaat ggtatcaaag acacattccg tagaggtatc gatacggtat tcaattggtt
    14521 ttcagaacta ccaacgaaga tgaagaatgc cattattggc ggtaaaaacg ccattgttga
    14581 tgcgttcaaa agtattttca acgcagcact taaagcgata ggtaaaccag ttaacgcaat
    14641 catccatgga gcttcatggg tactagaaaa attgggcgca gaacctctac aagaatggga
    14701 tgtaccacaa tacgctacag gtacaccagc aggtgggcac ccaatcaatg gtccaatgat
    14761 ggttaatgat ggacgtggag cagaaacagt tatcacacca gatggtagag ccttcattcc
    14821 taaaggacgc aatgttgttc taaacgcacc aaaaggaacg catgtcttga cagcagaaga
    14881 gaccgcgcaa cttcaaggtt ctaaagctcc taagtatcgt tacaaaaaag gtactaactt
    14941 ctttggtaac atgtgggata gtgtgaaaaa ggttgctggt aatgtaggtc acacaattaa
    15001 aaacgtagta ggtgacgtgt gggactttat ttcagacccg ggagcgttag ctagaaaagt
    15061 acttgggggg ttagatgtat taggtggatt gacaaagtat ccattagaag taggtaaagg
    15121 catcctatct aaagcaacaa gtgcactgac tgaaaaaatc actgggttgt tctcatctgg
    15181 taacttagat acctccgtag gaacaaatgg cgtctataaa tatttggctg atgttgctaa
    15241 gtctgtgatg aagaaattcc caggatttgt ggcaactagt gggtatagac caggtgaccc
    15301 ttattcacat ggtaaacgta atgccattga tattgcatta ccaggcgtca caggaggctc
    15361 acctagatac acagaagcag caaactacgc ttttgacaaa ttcgcttcca aaattggtta
    15421 cgtaatcact aatgggaaag ttcgtgaccg ttcaggacaa tcaggtcaac cagcaactgg
    15481 tgcatgggag ccatggcccg atggagatca ctatgatcat gtgcatttaa acggtgtgaa
    15541 agacccacaa aacactcaaa tttcaggaga tagcgtggga ggcagtgggg tagaaagatg
    15601 gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa tacagtactg caaacttaaa
    15661 tgcattacta aatcaaatgc gtacagagtc aaatggtaat cctaatgcag ttaacaattg
    15721 ggatattaac gccaaaaatg gaacaccatc aaaagggttg ctccaagtga ttgacccaac
    15781 attcagacag tatgcaatgc caggattcaa cagcaatatt tttgacccac tatctaacat
    15841 cttagcttca atcagatacg cactatcaag atatggctca ctaacaaatg cctatcgtgg
    15901 agttggttac gcaaacggtg gaattgtaaa ccaacatcaa attgcggaaa tcgcagaagg
    15961 aaacaagcca gaaattatta ttccgttaga taaggctaaa cgatcaagag cgatgcaatt
    16021 attgtctatc gcaatggaca aactaggagt ttctcctaaa acttatggaa atgcgacaac
    16081 tgtttccagt gattacgaaa tcctagaagc cattgaaaaa caggcggcaa caacgaatca
    16141 gctgttatcg ttattgctgg catttttcaa aggaaatagc cgaactgata gagatttagc
    16201 tttagatatt cagaagatct tggttaggag gatgtagatg cgaactgtat tactgagaaa
    16261 tagaataaac gaagaaattg atttgtccac agaagactat tttgctactg ggttgagtaa
    16321 tatggggttt gaagtaaaaa aagaacatgt gggacagtgg gggaatttca gagaaagcag
    16381 tgagagcgtt gaaatatctg aatttcagtc atctgtaatc atttctgtgc atgggtttcg
    16441 agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg gaaggtccgt ttgaactgga
    16501 atttgctttt gacggcgaaa caatggtaag aagatgcagt ctaaaatctt taagtaagac
    16561 tgaaatcgat ccgaaaacat cgttactaac agacacttta gaactatatt tcacatcaaa
    16621 ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg aatgtagtga aaacacgtgg
    16681 taaggttttt ccgtataaaa gatcttatat ctacactcaa aacttgtggg agaaaaaagg
    16741 tgtattcaaa ttcaataata attctgtata cctaacgaat agcaaggaac ggatgtcccc
    16801 gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat tgggaagtaa tccaaaactc
    16861 acaaatcatc gcaacggatg gatacttcat agatatgact gaaacacaga ctctagaagt
    16921 atcaagcctt tttgaagata caacggcgat tttaaaagat attgcagggg tagaatcttc
    16981 tgtctaccaa caacaggatt atacaaaaac taattttgtt caggccccaa ctggagaatt
    17041 cagcattgtg tttcatgttg ggggggcaga cgtagagatt gagttatacg aggagcgtga
    17101 tctgttttga ttttagcagt cacgcttttt catcgtgatt taatgcttta taacgaacat
    17161 ttattctcta acagttttga atttggtgtg gacgagatca acgaagaggc tagtagcttt
    17221 acgatggata agtacgttcc tgtaaaaaca ggcgattttc tactagcaaa atatattcct
    17281 agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg aagacgaaaa aatcagttgt
    17341 aaaagcttac ttagtttagc tgatagtgag ataccgactg cacgtgtgtc aggagataac
    17401 tacgaagagc atatccggcg gttgattgaa tactatttgc tgaatgatcc aacgaaacaa
    17461 ctaaaagata ttttagacgt caaagcagaa agtgcgacct ctcattcgta tcaagctacc
    17521 gatacgaata aacacaagtt aagtgcgtat attctcaatg gtttcaaaaa atacaacgta
    17581 aaatggtatt tcaagggaat tcaaaacaga aaaatttata caggcatacg tgctgtaaat
    17641 gaatcaattt acattaaaga caattcttct gaatttagcg attgggatgt gtttgttcag
    17701 gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg ataaagcaat gaaagatata
    17761 gagaaaccga taatactgtc aacatggtat ttggacgaag aaaacaattt gacacaagat
    17821 ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg ttaatatcta cgatcaaacc
    17881 gcagaagata aagcatccta cgaagatgtg gcaaaatcag agttgaaagg caatacgtat
    17941 tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa atttgaatgt cgaaacgatt
    18001 gaaacaggga tgtttgccac gatttcttat aaaggaaaga tatacaagtc ggttttgaca
    18061 gcttggcgaa tatcaagtga taaggagttt gtggaattga ctttcggaaa cataagaagt
    18121 cgttttatgg attattttga agataatggg ggataaaaaa tggttagcaa tgtggatgga
    18181 tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg ctagacttta tcacgtttta
    18241 tataatcgga aaaatcaggt tattgatggt tacgatcagt ctatgaattt atcttcaagc
    18301 ggattaacag taaaagttgc tgcaggagca gcgattattc aaggtcgtat ggtcgttgtt
    18361 cgacaagaag aaagtataac agttccagca aactcaagtg gttatatagc attaacagtg
    18421 gatttgacac aagaagttat acctggatct attcttccag aatcggaaga atatgaatgg
    18481 actaataatc aagtcaagct agaatttata acaaaagtta taaaaggtaa tttaaacaat
    18541 ggtgataagg tctataattt accactatgc tcagttaatt ctactggatc aactgtttca
    18601 atctcaaaga tatcggatag ttacgagctg actctctcta aaggagaaat tttgtggagg
    18661 gggactgcgt taatgcatga tattcaaact gtccaacctt caaaaaaaat ttggcagaca
    18721 gtttcaggtt ttttgttagt atggctccca tacgaaaacg ggcaagcaat tgaggataga
    18781 tatgtaacta cgccttttta taaggagcgt gtaactttta ctaatgtttt aggagaaatt
    18841 gtttcaggat ttgatgacta tcacaaaaag tggtttagta aacgaataaa ctataattcg
    18901 aatactaata tatttacagg tgcagcaagc aatgcaagtg gagataacgc aaatatggtg
    18961 cttaggtata tagtttcttt ttagttagga ggtatggaag agtggcaaat ttagaaatta
    19021 aattatctgc aaataagaga cagccttatc tacgtcaccg tgttgttggt agagttggtg
    19081 atggggggct cacaacaatc aatgtacaac ttcttgagga agatgaaatt acaccttttg
    19141 taattaatct aaacggtact ttgaaatttg tgggcgaagt ttcaaacggt aactataccg
    19201 agggagaacc agaaataatc gattcgacta atgggttaat tagttacacg ttcactaagt
    19261 caaatttcag cacgagtcat caattcaaac aagcatattt tgaatatgta gatcctaacg
    19321 gcaaaaaagt aacttttcag aacttcatca tagacgtgtt agaacgagtg gatattaatt
    19381 cggagcaagc gaaatactat atttcttcat tggaaaaatt acagagtgaa atgcaaacca
    19441 ctttcaatca gttcattagt gataaacagg tccaatacga tcaaatctac tcgaaatata
    19501 acgaattagt aagattgata aatgaatcag ataagcaagt aaatgatcgt attgatcaaa
    19561 ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac agcatcgact
    19621 ttgggggcta tgattattcg gggaatccga atttgttaag taagctatca tacgacttaa
    19681 ttgaaaatca aaatacttca gctggaacac tttctaaggg tgaaaactcg tttaaatata
    19741 ataagatatc agctgaagtg gaaggtggag tagagttata ttataaacga agaggtatag
    19801 ctaactggtt accctctaat aaaacgcttg taatgactgt taagcttaga gctggagcgg
    19861 actatagtcc agttgacgga aaacttatac tgattagata taggtatgtt gacagtggaa
    19921 ctggcaagat tgttttagac ttacctatta acagtaattc gataactcag gaatggaaag
    19981 agtttagtat tactggaact actccaacat ttagcccaca agcataccat ccttggatac
    20041 aatttagggc tcaagatggg gtacttgggg aaatagaaat gagctatgac atcaaaatcg
    20101 aagaaggctc tacagctaca ccatatcagc ctaacttact tgatgactct tactggctag
    20161 gtaaaacacc gttgggtgag aatttgataa cgaatgccaa gttcccaatt ataacacgaa
    20221 gcaatcctat ttctggtttt gatatttcag aagaattaat aataggtgaa acttatacgg
    20281 tatctttaaa agggactaag ccagctacta aagaattcca cttatactac ggagaagcaa
    20341 attaccagca gttgcagtat caggcaactt tacttcctgt ggaaggatta acagatgtgt
    20401 ggagtgccac ttttacagcc agaaatacta gtgaaacgac aaatttactt agagtagcgt
    20461 tatggcaaaa acctaattct gcaacatatg atactgttca aattgactgg ctcaagattg
    20521 aaaaaggcga cacacgcaca ccaaatatca gtcagtttaa atactttggt gaaggcttga
    20581 aagacagtaa caatccgaac gactacagct gggatgtcac accagagtat actgaaaaaa
    20641 gcttgaataa tacggttagt ctgactgaac cgcaatccat cgaaggattg aaaaatttcg
    20701 aagatggtat tcaatcaaaa ggaaaatctg tattgacatc agacgacaac aaatatgaag
    20761 tcgtaacctt aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa
    20821 aaaccgtcac tatttatttt tttgcgttga atgggaaaag cagtggtgga aatgattcaa
    20881 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
    20941 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
    21001 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
    21061 agaaaggcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
    21121 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
    21181 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
    21241 aatatggccg tactggcgga gagtatgcag gaaaagtatt ggacgacatg cttgtatccg
    21301 ttccttacac agatcagcca ccgctcgaag gggttcgttc agatggcgaa ccgctaacga
    21361 ttgctgatca gatgtttgat cctaaattga accaatggat tattttagcg aacgcgttag
    21421 atcacaacga tttaaacaat ctcaaagcga tgtacgaggc tcttgaacat gaaaacggca
    21481 acctaaaaca gctaaatgcc aaactcatgc taagcgatgt agcgattaaa caggaaaata
    21541 ctgcattgaa agaaaaagct gacagtttag cacaaatcaa ttcaaaaatg atgcttgctt
    21601 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
    21661 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt
    21721 actgacgatc aagttcgtct attcgttcca ctatgtatca cagatgaaga agcagataga
    21781 attattagca aagaagagag cgcatcttaa gtgatgcgtt tttttgttgg aaagttggtg
    21841 gaacatgaaa gaagaagcgc tccaagacgt tgtggagaga ttagtaagaa ttgaaacaaa
    21901 attagacaac tacgaatcac ttagagaaaa agctgatagt gcaaaagatt tggcagacaa
    21961 agcctactca gtagcgctaa acaacgcaga agatatcaaa gaaatgaaag ccaataataa
    22021 atggtcgtgg ggttacatga tcggtttagg cattacgatc attggctatt tcttgactaa
    22081 attgtaaagg agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg
    22141 gacggtttta acagtattac cagctgcatc tgttttagta gccacgttag gaaaagcgta
    22201 tggatggaat ggaacagata tgacagtact cactatcaat gcagtagcaa catttttagg
    22261 cgttatcact ggtgtgtctg catataactt aaaaaaatag gaggaaaaaa atgaagaaga
    22321 aaatcatttt atcattgagc ctaataatgg ctcttttttt attgccaatt aacgggttcg
    22381 cctatacgat caacaatgaa tttaatttag gtgtaaatga aggtagctca caagtagcaa
    22441 ataaccaata catcctattg catgaaacag caaacgaaac tgcgacagga cgaaatgaag
    22501 cgcagtacat gaaacgttct tggtacaatg cctatacagc gtatattgtt ggcgatggtg
    22561 ggatcgttta ccaagttgga caacctggtt atgtacaata tggcgctggt tcatatgcta
    22621 atgcaaatag tcctgtgcaa atcgaactgc aacatacgca cgataaagta acgtttgaaa
    22681 agaactacaa ggcatatgtt gaattagcga gagattccgc taataaatac ggtattccac
    22741 ttacattaga cacaccgtat aatcaaccag gaatcaaatc acatttatgg gtaactcaaa
    22801 acatttgggg cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg
    22861 aaaaactggc ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga
    22921 acaagcctgt cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa
    22981 cgaaccattc tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg
    23041 gatggcacat cgctaactat aaatacgagt atattttcat tatggactac aatactggga
    23101 aagaattagc tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata
    23161 atacttctgg aaacgttggc tatcatgtat cttttaacat gcgcaatttc cctaataaga
    23221 aagtatacgt catgatgcgg gcaacgaatg atccagaagg aaacactaaa ggcggtgcgc
    23281 aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg
    23341 ttgaggagca gtacatagaa ttgaaaatta acgttaaatc aaaaaaatat ttacaagatg
    23401 tttatttaag tagataataa aagaaaaaag caggtgaaat tgtgacagca gaaattggta
    23461 ttatgaataa aagtggaatc gtgttagctt cagattcagc atctacgatt ggagatagca
    23521 aagtatataa tactgcaaaa aaattattta ctttggattc catgcattct gtaggtatca
    23581 tgatatatgg taatgctgaa tttaatggta ttccttggga aataatcatt actcaatata
    23641 agaaaagcat tggtagttct gtttttaata ctttggaaga atacgcggat aattttatag
    23701 aatttgtcaa aacagcatct tttattagaa gtgaacagac cgagcaagaa caaatgattg
    23761 gggtttttca aaaaataatt tcaggtttat ttgagagtat agaagtagat attaactttt
    23821 taataagcca aggtacacaa atagacaaag atgtcctagt gaagttatta caaacaaaaa
    23881 tgaataccaa tttgtctcaa cagtcccaga cattcatttt agatattgaa aaggcgttat
    23941 ttcttagtaa ctatggagaa atattaaaag atattcttaa tagtatttct acaatggaag
    24001 gtgtttcaga agctatatca gaggaaattc aaagttacgt ttatgaaatt ataattagag
    24061 acgatgttta ttcttc
//
LOCUS       DASJTM010000041        23845 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJTM010000041 DASJTM010000000
VERSION     DASJTM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.041"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..790)
                     /locus_tag="U4B29_001519"
     CDS             complement(56..790)
                     /locus_tag="U4B29_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2106470.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="U4B29_001520"
     CDS             1117..1527
                     /locus_tag="U4B29_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN2106471.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="U4B29_001521"
     CDS             1520..2221
                     /locus_tag="U4B29_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN2106472.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="U4B29_001522"
     CDS             2221..3807
                     /locus_tag="U4B29_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106473.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="U4B29_001523"
     CDS             3826..4047
                     /locus_tag="U4B29_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN2106474.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="U4B29_001524"
     CDS             4044..4805
                     /locus_tag="U4B29_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN2106475.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="U4B29_001525"
     CDS             5185..5694
                     /locus_tag="U4B29_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2106476.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="U4B29_001526"
     CDS             complement(5769..6308)
                     /locus_tag="U4B29_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN2106477.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="U4B29_001527"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="U4B29_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN2106478.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="U4B29_001528"
     CDS             7321..8172
                     /locus_tag="U4B29_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN2106479.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="U4B29_001529"
     CDS             8200..8835
                     /locus_tag="U4B29_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN2106480.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="U4B29_001530"
     CDS             8855..9628
                     /locus_tag="U4B29_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2106481.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="U4B29_001531"
     CDS             10036..10833
                     /locus_tag="U4B29_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN2106482.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="U4B29_001532"
     CDS             10811..11224
                     /locus_tag="U4B29_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2106483.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="U4B29_001533"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="U4B29_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2106484.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="U4B29_001534"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="U4B29_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN2106485.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="U4B29_001535"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="U4B29_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN2106486.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="U4B29_001536"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="U4B29_001536"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN2106487.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="U4B29_001537"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="U4B29_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN2106488.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="U4B29_001538"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="U4B29_001538"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN2106489.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="U4B29_001539"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="U4B29_001539"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN2106490.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="U4B29_001540"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="U4B29_001540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN2106491.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="U4B29_001541"
     CDS             21908..22486
                     /locus_tag="U4B29_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2106492.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="U4B29_001542"
     CDS             22538..23020
                     /locus_tag="U4B29_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN2106493.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="U4B29_001543"
     CDS             23137..23511
                     /locus_tag="U4B29_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106494.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7787 a         4288 c         5121 g         6649 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttatttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttgggagcg tcaat
//
LOCUS       DASJTM010000042        23766 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJTM010000042 DASJTM010000000
VERSION     DASJTM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.042"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..687)
                     /locus_tag="U4B29_001544"
     CDS             complement(76..687)
                     /locus_tag="U4B29_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2106495.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(810..935)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1017..1733)
                     /locus_tag="U4B29_001545"
     CDS             complement(1017..1733)
                     /locus_tag="U4B29_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2106496.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1739..2308)
                     /gene="scpB"
                     /locus_tag="U4B29_001546"
     CDS             complement(1739..2308)
                     /gene="scpB"
                     /locus_tag="U4B29_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN2106497.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2292..3089)
                     /locus_tag="U4B29_001547"
     CDS             complement(2292..3089)
                     /locus_tag="U4B29_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN2106498.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3059..3430)
                     /locus_tag="U4B29_001548"
     CDS             complement(3059..3430)
                     /locus_tag="U4B29_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2106499.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3449..4336)
                     /gene="xerD"
                     /locus_tag="U4B29_001549"
     CDS             complement(3449..4336)
                     /gene="xerD"
                     /locus_tag="U4B29_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN2106500.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4432..4905)
                     /locus_tag="U4B29_001550"
     CDS             complement(4432..4905)
                     /locus_tag="U4B29_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2106501.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5010..5873)
                     /locus_tag="U4B29_001551"
     CDS             complement(5010..5873)
                     /locus_tag="U4B29_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2106502.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6069..8168)
                     /locus_tag="U4B29_001552"
     CDS             complement(6069..8168)
                     /locus_tag="U4B29_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2106503.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8388..9497)
                     /gene="rpoD"
                     /locus_tag="U4B29_001553"
     CDS             complement(8388..9497)
                     /gene="rpoD"
                     /locus_tag="U4B29_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN2106504.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9521..11362)
                     /locus_tag="U4B29_001554"
     CDS             complement(9521..11362)
                     /locus_tag="U4B29_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2106505.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11544..13859)
                     /locus_tag="U4B29_001555"
     CDS             complement(11544..13859)
                     /locus_tag="U4B29_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2106506.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14044..14904)
                     /locus_tag="U4B29_001556"
     CDS             complement(14044..14904)
                     /locus_tag="U4B29_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN2106507.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14963..15484)
                     /locus_tag="U4B29_001557"
     CDS             complement(14963..15484)
                     /locus_tag="U4B29_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN2106508.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            15762..16523
                     /locus_tag="U4B29_001558"
     CDS             15762..16523
                     /locus_tag="U4B29_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2106509.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16549..19140
                     /gene="mprF"
                     /locus_tag="U4B29_001559"
     CDS             16549..19140
                     /gene="mprF"
                     /locus_tag="U4B29_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2106510.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19328..20515)
                     /gene="tuf"
                     /locus_tag="U4B29_001560"
     CDS             complement(19328..20515)
                     /gene="tuf"
                     /locus_tag="U4B29_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2106511.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20883..21239
                     /locus_tag="U4B29_001561"
     CDS             20883..21239
                     /locus_tag="U4B29_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2106512.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21480..22484)
                     /locus_tag="U4B29_001562"
     CDS             complement(21480..22484)
                     /locus_tag="U4B29_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN2106513.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22650..23501
                     /locus_tag="U4B29_001563"
     CDS             22650..23501
                     /locus_tag="U4B29_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2106514.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23518..23763
                     /locus_tag="U4B29_001564"
     CDS             23518..23763
                     /locus_tag="U4B29_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106515.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         6593 a         4964 c         4155 g         8054 t
ORIGIN      
        1 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
       61 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
      121 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
      181 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
      241 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
      301 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
      361 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
      421 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
      481 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
      541 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
      601 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
      661 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
      721 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
      781 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
      841 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
      901 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
      961 cattatacat atctattcaa aaaacgcaac atctaacact tataaaaagt aagtgttcat
     1021 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
     1081 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
     1141 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
     1201 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
     1261 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
     1321 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
     1381 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
     1441 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
     1501 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
     1561 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
     1621 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
     1681 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
     1741 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
     1801 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
     1861 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
     1921 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
     1981 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
     2041 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
     2101 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
     2161 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
     2221 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
     2281 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
     2341 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
     2401 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
     2461 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
     2521 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
     2581 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
     2641 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
     2701 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
     2761 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gacgaattct
     2821 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
     2881 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
     2941 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
     3001 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
     3061 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
     3121 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
     3181 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
     3241 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
     3301 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
     3361 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
     3421 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
     3481 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
     3541 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
     3601 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
     3661 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
     3721 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
     3781 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
     3841 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
     3901 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
     3961 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
     4021 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
     4081 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
     4141 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
     4201 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
     4261 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
     4321 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
     4381 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
     4441 ctggtttctt ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg
     4501 atcttttact aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc
     4561 ctcttctact tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt
     4621 ggcgccttct tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac
     4681 aattttcaat tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg
     4741 actctttttt ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc
     4801 caaaagcaca agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag
     4861 ctgctgtttt gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac
     4921 aatttataat aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa
     4981 gctagttaag aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca
     5041 ccgttttctt gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct
     5101 cctttgctga taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg
     5161 atttgctgat ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata
     5221 gcttcatatg ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc
     5281 actcgacctt ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata
     5341 aacccaagat agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct
     5401 gttcctatca catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat
     5461 attttttcat ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc
     5521 atcaagcgat cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat
     5581 acgacaaagt ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta
     5641 gcagtaacga ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc
     5701 cgattttcat gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt
     5761 tcaccttctg atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt
     5821 tcattttcat caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc
     5881 tccctctata gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc
     5941 tacttgaata gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg
     6001 tcatactccc ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt
     6061 gcctaaaatt aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg
     6121 atgtaaaatg gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg
     6181 gcatatagat tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct
     6241 ccttcaacga tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt
     6301 gcccacgtcc atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt
     6361 ggctgattcg ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct
     6421 tgtgcgaccg cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt
     6481 gcttgttctt cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt
     6541 ttctctttat cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat
     6601 agatctgtat ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt
     6661 gctgaatcca ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc
     6721 aattcagcat attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct
     6781 gtgttatccg tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct
     6841 agatgatcat cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg
     6901 atcttagatt ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta
     6961 agactggata aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata
     7021 aattgatggt gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt
     7081 tgctgtcttt ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa
     7141 gcccagcttc ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt
     7201 gattttttct tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt
     7261 ttttcagtat ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg
     7321 ttctcttctt tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt
     7381 tcagcatcgg attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg
     7441 agtatttctt ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt
     7501 tcagtaaata cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt
     7561 tctttacctg tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc
     7621 tctactttgt tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc
     7681 tcttcttttg tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc
     7741 tcattatcga cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt
     7801 tcttcttgat tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca
     7861 tttttctcta tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct
     7921 ttttcgaata attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt
     7981 ggatcgttta attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct
     8041 gcatctgtat ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga
     8101 tgaccgcagt ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat
     8161 tttttcatca cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat
     8221 attctatacg tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt
     8281 tccttcctat agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa
     8341 taaaccaaac agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa
     8401 tcttttaatt gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc
     8461 tgacgaatac gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc
     8521 cggccatcat ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta
     8581 tctagtacat cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt
     8641 gttgcttcct ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc
     8701 gtttcaaggg aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga
     8761 agatccattt ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat
     8821 tgacgttgga tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc
     8881 gtacgtgctt ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag
     8941 gttgaaaact taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg
     9001 tttccttctt ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata
     9061 cttactacta aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct
     9121 tcgattttca atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt
     9181 tctttcagat acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct
     9241 tctgtttttg cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca
     9301 ccattttcat ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt
     9361 tcatctgcat tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact
     9421 tgtcgtttgg gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta
     9481 tctgttccat ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact
     9541 gtttcgttaa attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc
     9601 gtctggcttc ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc
     9661 tgatcacatc tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat
     9721 attcgatttc caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg
     9781 caaggttgaa ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat
     9841 atgcatcatg aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt
     9901 taaatgcacg atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt
     9961 tccgtgcagt ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat
    10021 tttgctgatt tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct
    10081 gaaactctgt tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc
    10141 taagttcaac tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt
    10201 tcccttgttt taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta
    10261 attcttggaa agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg
    10321 gaacgactac gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat
    10381 tggttgcttc aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct
    10441 tttccatttg ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt
    10501 tgataccagc ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc
    10561 cgtttttccg gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga
    10621 acagttctgt ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag
    10681 gtgcaagcca gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca
    10741 tcactctttg ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag
    10801 agcgttctaa caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac
    10861 gctcattagg tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac
    10921 tttgcaaata ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga
    10981 tttccgctgc tttttcatga gcagcaatca gtttccccgt agcagattca ggatttcgga
    11041 cagctatagt acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt
    11101 ttaccactgc ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc
    11161 cgcctttacc acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg
    11221 gtgtacgttc ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact
    11281 ggacatattg tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct
    11341 cttgaggaat ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta
    11401 acagaagata tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat
    11461 tttaaaggac acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata
    11521 acacatttct atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata
    11581 tcagccttcc agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat
    11641 cctacgctct ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct
    11701 acatatatcc ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa
    11761 caagctttta caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta
    11821 gtttcagcat ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact
    11881 actacaagca aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg
    11941 atttttcttt tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa
    12001 tcaatcagcg tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata
    12061 atcgataaaa taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca
    12121 ttgttgacca ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc
    12181 aacaatacca gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg
    12241 caatctgctt ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt
    12301 ctgccgcttt ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca
    12361 aaaaccgtat agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga
    12421 tcgacatacg catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa
    12481 ggattgtctc cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt
    12541 tcttgagcgt tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat
    12601 gctggttttt cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag
    12661 ccattcgctt gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca
    12721 ggcgctccta cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca
    12781 gaagaaaatg cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc
    12841 atagcccgca tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct
    12901 gggatggctt tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct
    12961 tcagtaatgg tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt
    13021 gaatacatgt cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat
    13081 gcaatactta ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat
    13141 gcaataacag aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg
    13201 atccccaaag gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca
    13261 agttcagaac gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta
    13321 tctgcaccta ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa
    13381 agcaattcag ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca
    13441 tttgcttctg cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg
    13501 atttttacca aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc
    13561 cggatcacta gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca
    13621 tgtatttctt gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg
    13681 ttggaatacg cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat
    13741 aacgtcaata tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag
    13801 tcattttgct ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt
    13861 ttctttttct ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt
    13921 tccaagatta aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta
    13981 caaaaaagat gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta
    14041 tctttactta tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat
    14101 aaaaagaaca ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat
    14161 gatcataatc gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc
    14221 ctttgagaaa atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat
    14281 tccgatcggc acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata
    14341 caatcccata tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc
    14401 aatcaatccg ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa
    14461 ttgagcgccg tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac
    14521 atcagaagta tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc
    14581 gatacatccg acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac
    14641 taaaaaagaa agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa
    14701 aacattttct ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat
    14761 gccaaagaca gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat
    14821 aaacattccc ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt
    14881 ttccattttt tgcctttttt ccactatttg cgtactcctt ctttcattta attttattca
    14941 tgattgaaca tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt
    15001 cattcatttt gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc
    15061 cattgtaaag caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta
    15121 aaagtgggct cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc
    15181 catgattttt tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc
    15241 ataaatcgca tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt
    15301 gatcaatact gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc
    15361 tctgcatgag atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat
    15421 tctcgtagtt ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt
    15481 ccactcgttt taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg
    15541 tcagaatgat catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat
    15601 cacctagtta ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc
    15661 ttccttgatt tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct
    15721 tacgaaaatg atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta
    15781 tactttacac agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg
    15841 ggagcgaaat ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca
    15901 atgacggcag cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg
    15961 tgtatctagt agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt
    16021 ttcagttaat ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag
    16081 attttcttgg atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta
    16141 aaaaagttca tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt
    16201 ctgccagagt catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc
    16261 caagtcttcg caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag
    16321 aaaatgattt aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa
    16381 tcaagctaaa acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg
    16441 tgaaagaaca aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc
    16501 ttcaatttct ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat
    16561 accatacaat ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc
    16621 attgtcatta ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta
    16681 ggagaaacgt ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt
    16741 atctctgtgt tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag
    16801 cagaatcctc gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc
    16861 ggtttcggcg ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag
    16921 agcagaaacg tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct
    16981 atttacagtc tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag
    17041 caatattgga tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact
    17101 actttcaaaa agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg
    17161 ctagtttcat tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta
    17221 atggaaatcc ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata
    17281 ggaatcgtgt ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg
    17341 ttgtccgcta ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg
    17401 ttctactaca tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc
    17461 acttttgatc agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa
    17521 atcgtggttg tcttattata cttttctgga atcatgctag tactttctgc cactatcccg
    17581 caagctttta cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt
    17641 atccaatttc ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca
    17701 gcacgtgtga aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat
    17761 gttttattaa gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc
    17821 attcttgctt ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt
    17881 acgatcgatg gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac
    17941 aatctgccgg atttccctca tcggcgtcac cattttattt cctttttctt atttccttct
    18001 gaaaagattt ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta
    18061 tttgttcatt tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag
    18121 gccctgaaga tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa
    18181 gataagcgga tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct
    18241 tgcttcaata ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca
    18301 gaagcaatcg aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac
    18361 gaaacaagcg aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg
    18421 ggggaagaag cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc
    18481 actcgtgctg tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca
    18541 ccattttcta ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca
    18601 cgaaaggaaa aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct
    18661 attgcagttg tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact
    18721 tacacgaaca ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct
    18781 tctggcagta tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc
    18841 caatggttca atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt
    18901 ttacaggaaa ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat
    18961 ggattaagag aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat
    19021 tctagagata gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca
    19081 gttgagcaga cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa
    19141 aaactgcata ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga
    19201 aagttcatgc ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag
    19261 aaatcctgtt tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc
    19321 gcgtttctta tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac
    19381 gaattgagaa tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg
    19441 tcacgttgtc accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa
    19501 catcagttgt gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc
    19561 caccttcttc ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg
    19621 aacctggttt agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca
    19681 acacgcctac gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac
    19741 ctgttacaac tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga
    19801 cacgaacagc accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga
    19861 agacgtcttc cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat
    19921 attcatctac tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca
    19981 atgcttttaa tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc
    20041 tcaataactc gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat
    20101 caactttgtt caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt
    20161 gttcacgtgt ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca
    20221 tttgtgctgc ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat
    20281 gcgcgtagtg gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac
    20341 gttcacgttc ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt
    20401 ttttgcctaa tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc
    20461 caatcgtacc aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt
    20521 acaaacctcc taaataatat agatcactga aaaatttcag ctctcacctt tttattatag
    20581 tctttttttg actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta
    20641 agcttattat acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc
    20701 gaaaaaagta ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc
    20761 cttaactttc ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg
    20821 ccaacaactt tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt
    20881 acatgaaaaa attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg
    20941 cagcttatca ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg
    21001 aaatgtcagc tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag
    21061 cggcttataa taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc
    21121 cattaaagat gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct
    21181 gggaagaaat aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt
    21241 aaatggtctt aaaattttat tagataaaaa gaggctggga cagaagcgtt taactccaag
    21301 aaataagaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa
    21361 gtgttcttta cttttgtctc acgctctttt tttgttatat tcgtcttttc cgatccttct
    21421 cttgacacaa ttcatttaac tgatcactgg tacttgaaca cctaatattc tagtcatttt
    21481 tataaacgat tcaatacttc atccacattt ttgatcatca cggatatcgt accatccgga
    21541 ttattcacaa attcaatcaa atctgggttg cgatatacat ctactggtac gatcagttcg
    21601 atcccgtttg atagcttgaa tttttgtttt ccaaattttt tctcagaaat ttcttgtact
    21661 tcttttacag gaggtgcttc tgggacaaat ccttgctctt gtacttcttc tttaaaagcc
    21721 atttttgccg tgatgttttc cttgaagact ttttccgcaa tcatctcatg atccaaccga
    21781 cctttttctt ctatcgtatc atagacagct tctttcaaat cagccatgat atcaaattct
    21841 tctgtctcaa atttttttcc aagatgcttg gctacttttt ggatcacttt tacattttct
    21901 tctaaagaag gtgcaggaac actttcgatc actcttccag aaaaataaag ctcttttttg
    21961 ccagaaaact catatttttt ttcaattaat tcgtaggtcc aatcagtcaa attcacagcg
    22021 aatgcttcat ctgctttttg tgttttcgcc cctagaattg ctcgattcaa aatcaagcgg
    22081 ttgtcgatcc ctgcttcgtc tgcttcaaca aagtgagtgt aagcctcacg ataattgact
    22141 ttcaataaag ataagtacat cactgtatct aattcataca agacaacaaa tacatctgca
    22201 ctaggtgctt cttcactttc ttggtagata tcgaaccacc gctgaacgat tgcttcactt
    22261 gcttccacaa aattttcttg agcgatttta agtgattcaa cgatcatgct ttcttctgct
    22321 agttgtccag tttttgtttg agcagaagag attttttgga tcttcttttg aagaaaatca
    22381 cgaatatatt cttttgtcag atccagctct ttttgtgaaa agactggaga accagtttct
    22441 ctgtcaataa tatgcagaat tgcttttttt aaatagatat ccattttctc attcccttct
    22501 gtcttagttt acggaaaaaa aatcaaaaat accagaatca aaatcatttt cttttaaaca
    22561 aaaatttcaa gagtagaaac aaaatggatt ctttttttcg aaaaatgcgc tacacttata
    22621 gttgaacgaa taaaaaagga gctgaaagta tgaattcatt aacaagcact tatcgtctat
    22681 caaatggtta cgaaatcccg gtagtaggat tcggcacatg gcaaacacct gatggggatg
    22741 tcgctgtttc ctctgttaaa gaagcgctgg ctgcaggtta tcgccatatc gatactgccc
    22801 aaggatacaa gaatgaagaa agcgtcggcc aagcgattaa ggaaagtgga attcctagag
    22861 aagagatatt cttgacaaca aaattatgga atgcaaacca cagctatgaa cttgtgatgt
    22921 cttcttttga agaatcattg aagaagctcc aaacagatta tttagatctg tttttgatcc
    22981 actggccaaa ccctgttgct tttcgtgata attgggaaca agcaaatgca gatacatggc
    23041 gagcatttga agaactgtat gaagcaggta aaatcaaagc aatcggtgtg agcaatttct
    23101 taccccacca tctagataca ctagcaaaaa cagcaaaaat tatgccgatg gtcaatcaag
    23161 ttttccttgc accaggcgaa ttacagacaa ctgtagttga gtatgcaaaa aaacatgata
    23221 tgatcctcga agcatatagt ccattaggca ctggtaaaat cttcgatgtg cctgaaatga
    23281 aacaaatcgc tgaagcacat gacaagacga ttgcacaagt tgctttgcgt tggtcgctgc
    23341 aacacgaatt ccttcctctt cctaagtcag tgacaccaag cagaatcaaa gaaaatactg
    23401 aattatttga ttttgaatta acagaagaag aaatgaaaca aatcgatcag ttagatggtg
    23461 tagtcggcaa agcaaaagac ccagatacta cccaatttta agtcgaaagg tgattttatg
    23521 gatccaatcg aacgcttgaa ttctctctcg gaagaagtta cccagacttt tcattccgat
    23581 tttgtctttt tgatcgatgc agagaaaatc caacattttc ctgcaagaaa ctggacacat
    23641 gatcaaatca tcgaagaact gaaaaaacgg ttcgatcatt cgctaatggt caaaccctgg
    23701 cacgaacatg aagtcattta ttcacctgaa ctacctgttt ttgccttgat accgaaaaaa
    23761 tagaaa
//
LOCUS       DASJTM010000043        23214 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJTM010000043 DASJTM010000000
VERSION     DASJTM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.043"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="U4B29_001565"
     CDS             complement(104..1336)
                     /locus_tag="U4B29_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN2106516.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U4B29_001566"
     CDS             1591..2160
                     /locus_tag="U4B29_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2106517.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U4B29_001567"
     CDS             complement(2338..2778)
                     /locus_tag="U4B29_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2106518.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U4B29_001568"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U4B29_001568"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2106519.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U4B29_001569"
     CDS             3732..4655
                     /locus_tag="U4B29_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2106520.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U4B29_001570"
     CDS             4731..5870
                     /locus_tag="U4B29_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN2106521.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U4B29_001571"
     CDS             5863..6663
                     /locus_tag="U4B29_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2106522.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U4B29_001572"
     CDS             6663..7490
                     /locus_tag="U4B29_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2106523.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U4B29_001573"
     CDS             7468..8202
                     /locus_tag="U4B29_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2106524.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U4B29_001574"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U4B29_001574"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN2106525.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U4B29_001575"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U4B29_001575"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN2106526.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U4B29_001576"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U4B29_001576"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN2106527.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U4B29_001577"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U4B29_001577"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN2106528.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U4B29_001578"
     CDS             11787..13820
                     /locus_tag="U4B29_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106529.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="U4B29_001579"
     CDS             13864..15144
                     /locus_tag="U4B29_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN2106530.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="U4B29_001580"
     CDS             15354..16160
                     /locus_tag="U4B29_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106531.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="U4B29_001581"
     CDS             16172..17392
                     /locus_tag="U4B29_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106532.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="U4B29_001582"
     CDS             17382..18968
                     /locus_tag="U4B29_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2106533.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="U4B29_001583"
     CDS             19007..21145
                     /locus_tag="U4B29_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2106534.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="U4B29_001584"
     CDS             complement(21513..22496)
                     /locus_tag="U4B29_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN2106535.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..>23214
                     /locus_tag="U4B29_001585"
     CDS             22874..>23214
                     /locus_tag="U4B29_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2106536.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLS"
BASE COUNT         7272 a         3951 c         4788 g         7203 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catt
//
LOCUS       DASJTM010000044        23104 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJTM010000044 DASJTM010000000
VERSION     DASJTM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.044"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..269)
                     /locus_tag="U4B29_001586"
     CDS             complement(<1..269)
                     /locus_tag="U4B29_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106537.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVAT"
     gene            complement(273..890)
                     /locus_tag="U4B29_001587"
     CDS             complement(273..890)
                     /locus_tag="U4B29_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106538.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(890..1798)
                     /locus_tag="U4B29_001588"
     CDS             complement(890..1798)
                     /locus_tag="U4B29_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2106539.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(1811..4570)
                     /locus_tag="U4B29_001589"
     CDS             complement(1811..4570)
                     /locus_tag="U4B29_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106540.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(4567..5271)
                     /locus_tag="U4B29_001590"
     CDS             complement(4567..5271)
                     /locus_tag="U4B29_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106541.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(5283..7592)
                     /locus_tag="U4B29_001591"
     CDS             complement(5283..7592)
                     /locus_tag="U4B29_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2106542.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(7787..8251)
                     /locus_tag="U4B29_001592"
     CDS             complement(7787..8251)
                     /locus_tag="U4B29_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106543.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(8251..8877)
                     /locus_tag="U4B29_001593"
     CDS             complement(8251..8877)
                     /locus_tag="U4B29_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106544.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(8884..9249)
                     /locus_tag="U4B29_001594"
     CDS             complement(8884..9249)
                     /locus_tag="U4B29_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2106545.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(9239..9577)
                     /locus_tag="U4B29_001595"
     CDS             complement(9239..9577)
                     /locus_tag="U4B29_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106546.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(9567..9893)
                     /locus_tag="U4B29_001596"
     CDS             complement(9567..9893)
                     /locus_tag="U4B29_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2106547.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(9871..10152)
                     /locus_tag="U4B29_001597"
     CDS             complement(9871..10152)
                     /locus_tag="U4B29_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2106548.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(10154..11518)
                     /locus_tag="U4B29_001598"
     CDS             complement(10154..11518)
                     /locus_tag="U4B29_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2106549.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(11531..12100)
                     /locus_tag="U4B29_001599"
     CDS             complement(11531..12100)
                     /locus_tag="U4B29_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN2106550.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(12072..13259)
                     /locus_tag="U4B29_001600"
     CDS             complement(12072..13259)
                     /locus_tag="U4B29_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2106551.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(13323..15050)
                     /locus_tag="U4B29_001601"
     CDS             complement(13323..15050)
                     /locus_tag="U4B29_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2106552.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(15047..15499)
                     /locus_tag="U4B29_001602"
     CDS             complement(15047..15499)
                     /locus_tag="U4B29_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN2106553.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(15611..15991)
                     /locus_tag="U4B29_001603"
     CDS             complement(15611..15991)
                     /locus_tag="U4B29_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2106554.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(15988..16374)
                     /locus_tag="U4B29_001604"
     CDS             complement(15988..16374)
                     /locus_tag="U4B29_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106555.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(16376..16654)
                     /locus_tag="U4B29_001605"
     CDS             complement(16376..16654)
                     /locus_tag="U4B29_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106556.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            16702..17430
                     /locus_tag="U4B29_001606"
     CDS             16702..17430
                     /locus_tag="U4B29_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN2106557.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(17576..18052)
                     /locus_tag="U4B29_001607"
     CDS             complement(17576..18052)
                     /locus_tag="U4B29_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2106558.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(18012..18359)
                     /locus_tag="U4B29_001608"
     CDS             complement(18012..18359)
                     /locus_tag="U4B29_001608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106559.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(18346..18531)
                     /locus_tag="U4B29_001609"
     CDS             complement(18346..18531)
                     /locus_tag="U4B29_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106560.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(18532..18804)
                     /locus_tag="U4B29_001610"
     CDS             complement(18532..18804)
                     /locus_tag="U4B29_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN2106561.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(18801..19004)
                     /locus_tag="U4B29_001611"
     CDS             complement(18801..19004)
                     /locus_tag="U4B29_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106562.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            19129..19341
                     /locus_tag="U4B29_001612"
     CDS             19129..19341
                     /locus_tag="U4B29_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106563.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(19374..19568)
                     /locus_tag="U4B29_001613"
     CDS             complement(19374..19568)
                     /locus_tag="U4B29_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106564.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(19581..19763)
                     /locus_tag="U4B29_001614"
     CDS             complement(19581..19763)
                     /locus_tag="U4B29_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106565.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(19760..19900)
                     /locus_tag="U4B29_001615"
     CDS             complement(19760..19900)
                     /locus_tag="U4B29_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106566.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(20390..20755)
                     /locus_tag="U4B29_001616"
     CDS             complement(20390..20755)
                     /locus_tag="U4B29_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106567.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(20795..21097)
                     /locus_tag="U4B29_001617"
     CDS             complement(20795..21097)
                     /locus_tag="U4B29_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2106568.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(21097..21396)
                     /locus_tag="U4B29_001618"
     CDS             complement(21097..21396)
                     /locus_tag="U4B29_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106569.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(21389..21553)
                     /locus_tag="U4B29_001619"
     CDS             complement(21389..21553)
                     /locus_tag="U4B29_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106570.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(21559..21828)
                     /locus_tag="U4B29_001620"
     CDS             complement(21559..21828)
                     /locus_tag="U4B29_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106571.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(21840..22589)
                     /locus_tag="U4B29_001621"
     CDS             complement(21840..22589)
                     /locus_tag="U4B29_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106572.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(22586..>23104)
                     /locus_tag="U4B29_001622"
     CDS             complement(22586..>23104)
                     /locus_tag="U4B29_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106573.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
BASE COUNT         6426 a         4922 c         3571 g         8185 t
ORIGIN      
        1 gtagctacag ctgtagttgt ggcatttatt ccaggatcac ctttatctcc ctttggtaaa
       61 acaaaattaa atctagctgc agatgatgtt cctacattcg taacagaagc ggttgaacca
      121 ctagaaacgg ttcctatggt aattgttgct gcttggccag gatcgccttt atctcccttc
      181 accgttgttg gtttgccttc tatagcattc caatgagttt gtggataaac ctgtacaccg
      241 ctttgtttta tttttacgat atctgtcatt ttctatacct ccccgatttt ttcaaaagta
      301 aaatttggaa ttctttcgtt tgtgtaattt tctgcttgat ttacagcttc ttggaatttt
      361 tgatctacat atgactgatt aacaccacca gtcccactac cacctgtaga actaattgtt
      421 ccatcttctg caattgatat atttgcacct gcttttaata tttttagaga ttctaatttt
      481 ccctttaatt cttcagagaa attgaagtct gtttgcttag ttgcagataa aacaccttct
      541 tcagtaactt ctaagagttc cccaactttt atacctccta attgttcagg agtagcaatc
      601 ggcaaaatat atgttccacc ttctccattt acgatctttt gaaacatttc agcagtaaga
      661 ataccatcgc ttgtctcgct tgcataaggt agttcagtaa ttgcattctc taatcctaaa
      721 tcagctttcg ttaaaatgac tgcaccatat ttaccattaa ccgaaagaac ttttgattgt
      781 cctgatatca ttttttctaa gcctaaaact gcagaaacat gtgtaattgg cataaattga
      841 cgtttaacac cagattcatc agtctccatc attctcttta ctttaaccat caaatcaccc
      901 caactttctc aagagtgaaa acattctgtt ttggatcatc aacagttgca ataatcaaag
      961 ctccttcttc aattggaaat tctactgttt caattttttc gacttcgtga ttatctgaaa
     1021 agagatcgtc ttgaagaata tcagtcactt caatttcacc gtattcaatc gtgaaaagtg
     1081 ctgctttcat tttttgatac aaataattta gatcggatag gaaacgttca gaaatcgatt
     1141 catggcgtac accttgaaga tctacacgtg catccattaa ttcggctaac attactccac
     1201 ctggatcgat agacttcaaa atatccttga ttgattcaaa ccatgacaaa taatctgatt
     1261 cttgtccttc tctccaatct tggaaactat tctcctgttc ttctctccag cgatcaaatt
     1321 cttctttcct ttcattcatc cagtccgtaa aatcgccttt attttcatta ataaaatctg
     1381 tcatatcagc aattaaatct tcaattgatt gccaataaga acccatttct ccttctgttt
     1441 ttgaaacagc attgatgaca aaataggaga agtcttgagt tgttccaatc aagtcttctc
     1501 ctttaaatat aatgaaattg gctgtttgtc gatgcaaaca ctgcatggaa tatttatcaa
     1561 aaatatattt gattttccct tttttagcat ccacaattct tgtttctaat tggactggat
     1621 attttccgcc aacaactgat tcaaaatata ccttacattc tgataaatca tatggaagac
     1681 cattttcgac aattgtagct tccataactt cagtgttttt attgccttgt cgaacttgaa
     1741 tcatccccac gtaattatag ggttctgttg tacttaatat gacattccac tttgccatta
     1801 aatcacctcc ctattttggt ggtatgacaa tggaagaaat tgcacttgca ctataatact
     1861 ggcgatctaa ctttccacaa atcataccta actctgtgtt ttgttcataa gtttgcatac
     1921 gaccattagc taagcctcgg ataacgccag tatgtccata agttccgtct gcaaaccatg
     1981 aacccacttg tccacctctt gcccaattaa taattgcacc aactactaat tgatcatatc
     2041 tagggttttg aatcactttc caaccaacag cagaccaatc ataggcaata ccaatatcag
     2101 atgctgcaga agtatttcct atcacatgag ttaaaccata ttttgttcca gcacccatgc
     2161 cacaaccacc cagatatcct gaatattctg cagacaagcc ataacactga ccattgccaa
     2221 ttcgttgtcc aatcaaagac tctaaatgtt ttagtcctgc ttctccagta gctcctcctg
     2281 gttttaaatc tttaaatttg ttataccagt taactgcata atcttgacgt tcaggatgtg
     2341 tcgctgccgg acgttcatag tttcgttcaa aagcatatgc tgcttgtctt ggatcagtac
     2401 aggccttaaa tccatcaacc gtcgtaggtt gtactacgcc catccattgt ccatttgtaa
     2461 atgtccaaat aagcaatcga acttgtgcat ctaagctcgt tattggttct ttaatacctg
     2521 cagcattaaa taagttttga acataaactt ttccatccca tgttgctgga ccaacaagag
     2581 gatatgaaga accatcccac tgaactaatc cgtatgctgg tccacctatt tgaacagtat
     2641 caggatcaaa agttcctcct gtttcttgtt gaatgttccc caatattcca catgcagatt
     2701 gtttcgtaaa tccgttatta cacaaaatat cgtatattct ccaagctctc ttttctgcat
     2761 ctgtttttaa ttcatcagga tacccaccag gtgtttctcc tccgccagat ggaccgccat
     2821 tttgtcctgg tatcacttct ttacctctaa cagtaagttt tccctgaata tctaaatcac
     2881 cgaaataata tgcttttccg tttcctaata acacaaatcc tttttctgca gttggagaaa
     2941 ttaaaatgta ttttccatcg ccatttgtac gtattaccaa agaattttcc tcaagaggtg
     3001 taggtgttac agcatttgga aatggatttc ctgctgaatc tgttgttccg atcgttccaa
     3061 tggattcttt tctattccag aattccatac cttttttggt tagctccatg atttttttgt
     3121 tttcattcca aatttgaagc gtgcctttta caagttttaa cacatcacca taagcattaa
     3181 aggaagtttc gaatacttct gcatttattg ttcctacttt gatgaagtca gcaacaattt
     3241 ctccttttga tgtgatagca ataccgaacg ggccatttac tccgttgtct gaataaccta
     3301 agccatttaa attccaacgc cacactcgct ttgcattcgc cacatttgga gtatccatga
     3361 taagaatctc tgatggagct ttttctggac gaaaaacgac atgcccacca gaattaccag
     3421 tgatccatgc cgtcgcattc aacacatttt gaactaacgt ttctgttcga ttgtcgattt
     3481 tctttttcaa ttcttgtgct tgagtattta ctgccgaagt gtacagtgat aaatcattac
     3541 ctaaaacaat atccttatat ttcccaagcg ttgggaaata agtgtattcg accatacgct
     3601 cttttatctc gatatctagc tctttcgccc taacatgagc gacatcacca aagtgtaaag
     3661 acgctagctc ttgatacatg tcaccgtatt cgagtgtgtg ttccaacgcc accatactga
     3721 ctgtatgagt agcttttggc tcatggatac gatcattgtc aaataaggtt ttgccccatt
     3781 tgatcaattc atcaactgtt ttacaatctc cgttttcacg tttggcaata cgtcgattct
     3841 cgttggttac tccatcaatt tctaaataac catattcgat aggttctttg tcttcatcat
     3901 aatcgttatc aggaacaccg ccaaccaaat acaaactatt gacaattgat tcttcatcaa
     3961 tttcttcttc gattgcttct aaattgatgc caaaatcaat ccgaaaacca ttatctgatc
     4021 caatctgttt taccagtttc aaatcatagt tatccatatc aagttccgcg ctagtcacgc
     4081 cagtcaaatt ttgattaccg ttatttgaac caatgattgc ttcaactggc gcgacttgtc
     4141 ttgctgtaaa ttggtgcgtt gtccctacat tcgaaagata gttaaatttt tgatcaaatg
     4201 ccaaactgtt tttaagattg gtcatgatct gagacccatt gccgttatcc gtaaacgact
     4261 tgacaataaa gttcttattg gccataaaac caatatgccg agctgttaca gaaacggagg
     4321 tcaaattctt tttgacatta taaatttcaa aatattgcca tgatccatct ggtaccttcg
     4381 cttttataaa atttcctttt tttaaataag agcgatactg tccgcttctt gaatagttac
     4441 cataaaaacg atattggcca ttcagcacac gattgatttc tggcaaatct tcccaatcaa
     4501 ctaacgacgc tccgttcacg ctcaaatcat ctggcatttt tttataagca taaataaatt
     4561 cttgcgtcac agatacacac tcctattcca aaaccgcact tctttaaatt ttccggatat
     4621 ttttacctga ttccattcag gctgcaatac tggccaatca ccacgcgtaa ataaattcaa
     4681 accttcttgt attgcttttc caagctgagt atcaaccacg attgttgcag ctaacgtatt
     4741 aagtattgtt aggcttttat caccgacagc gatcgttata tctcctccgt tcgattctat
     4801 ttccagatag ggatgtgcaa tttcgtcacc atgatcaaaa atatccataa cactagaatg
     4861 aaaaatttta ggtgcttcac cgatttttct tttgagtggc tgacaacgaa aagtaacatc
     4921 gaatgtataa aagaagcccc actcgttttc gaaagggact tctttatcca tgctgcagat
     4981 agcctctaga tacttgtctg gatcattatg tgtgattaat tggcttttac cagtgagcca
     5041 tcgtttgact tctctcagtt tcgaatgtgg aatagtgatc ccttctattt ccaaatcaaa
     5101 aggttcatag tcatcaaacg tctccgtcaa ttctccgctt cggccctgaa tcgtgtaagt
     5161 ttcgtatcgc ttgttcggca taatatctgg aagctctgcc tcaatgatgc aattcatatc
     5221 aattactgca ttccgatttt tccaaataaa atttggttca tctggattca taaattgtcg
     5281 actcaaatag gaacacctcc caaatcacga atagcctgtt tgtttgcttt agcaaattta
     5341 cgattcatac gatctaactc agatggattg tttgcatcga ctttgccaat gtgtatatgc
     5401 tgttcaatgc tacctccaga aactcttcca ctgatccctt tgcgtttttc ttcgtctgat
     5461 aatggagtaa ctgtggtttt accattcttg gcagtcagta actcaggtcc agcttcacca
     5521 acaatcgctt gtccattgat tagatgaccg ccttgtgcta aataaggcag ttttgaaata
     5581 gaaaatgatt ttccacctac accaggaacc cattttggta tctttatatt attcaatcca
     5641 ccaataaatc cattgatcaa tccaatcatg gcattaatag gagctttccc tacagcaaca
     5701 ataccatcga aaatacctcc aaaaatatca actagacctt gccatgcttt tgaccaattc
     5761 cctgtaaata ctccagtgat aaaatctaga aaaccactga aaattcgttt tccagcatta
     5821 taaaaattat tgaaatttgc tataacacca tcgaaaaatc caccaaaata accacttaaa
     5881 aaattaaata tttcaacagc tacatctgaa acacctttga agaaagaatt cactccatca
     5941 tgaaaccatt taacgttatt atatgcccaa attagtccag taactaatgc gccaatagct
     6001 ataactgcta atgcaaacca gcctcccgac attccaaata aaccagcaag tccttgccaa
     6061 actcctataa aacttttaac ttccccaacg atttttgtaa cagatcccat aagtgaacca
     6121 agaacgatta gtactggtcc aactgctgcc gctataccag caattgttat gatccaattc
     6181 tttgtatctt gatctagaga cccccaccat ttagagaatt cctgtaataa ggaagttgct
     6241 ttttcaaaaa aaggcaaaag gcttacttgg actgcttcac ctacgtcagc cattgctaat
     6301 tttgcattgt tcatagcttg atcggcttga tcaattgggt cgagagtagc atcgaatgtg
     6361 tctcctacag ctcccccact ttctccagca gtttttgcta aatcttccag gttcaaagta
     6421 cctctacgaa ttgcatcggc cattctagga ccgcctttag tcccgaatac ttctgctgct
     6481 gcattgatgg cttctgtttc agaacctgca ttttttacct tgtcttgtag ttcttctagt
     6541 ccttgactta aagattttcc gtctttcgca taagccactg ttgctttaga taaactactt
     6601 aaagctgcgc ttgaatcaac accagattgc tcaaattgtc caagtaaagt gaccccttca
     6661 ccaaagctca accctaattg tttaatttgt ggtgcgccat caattgcttt ttgcatcaag
     6721 tcgtctacag attgaccagt attctgcgat gttttcgttg ttacatcaag gacagaattc
     6781 aaatcatcat attctagtcc ataagcatca atagcttgtc gagcagatat tgctgattgc
     6841 gaaacatctg tatcattgat ttcagcgtac tgtaggagat aattggttga atcttccagt
     6901 tttttatcca tgaatccaaa ttgtgtattt acctcaccaa tagcttcccc aaccgtttgt
     6961 aaaggtaaat gagtatttga accaacgttt ttgaaagact gtgaaagtct atcagcttga
     7021 tcacctgttg ctccggtttt tgtaatgatg gtatcaagtg cctcgtcaac ttcaccaaat
     7081 gctgcaagtc ctgctgctcc tgccgctaca ataggcgcgg ttactccaac agacattttt
     7141 tcaccaacgc cttttacttt ctcacctgtt tcttcgattt tctggagttt ttttgctgta
     7201 tcaacagaaa cgtcaccttg ttctttaagt gcatcgttcg tctgttctaa tgctgtacga
     7261 agtttatttt ctccggtttc agaattgagt aattgtttat aaagtttttc tgattcagca
     7321 gaatattcac cggtttcttt gacagatttt tcatattcct ctctcagcat ttgagatcgt
     7381 tgttcagcca atcctaattg tttttctaat ttttgtttag ccgctcgcaa tttttctgtt
     7441 tgagttgcat ccttgtccat cgcagacact tggtttttgt actcggcagc ggctaagttc
     7501 atttctttgt tgatttcttt gattgttttc gaataactga cttcgccatt cgttttaaaa
     7561 ttaagaacga catcagattc tttaccagcc atttatctta gcgctccttt ctaccaccaa
     7621 ggcgatttat ccatagttac agattgaggt ggttcaaaat cggtgttttg ttgtaaccac
     7681 tgtaaataag atttgagcca caagttaggt gtagacttta aaaagaaact ctcactccaa
     7741 ttcaatagag tgagagcaac gtaaatataa aaactccaag gagttcctat ctcttccgat
     7801 tctttttgtt tttctttctt tttgcttgcg ctttttgaaa gtcttgtggc ttcttggatt
     7861 tttttaggtc ttccacctga aatgtctggc tagcaaaaat ttccatacag gtactataag
     7921 cagacaacac ctctccactc attcccaaaa atttgaaaat agtttctgga tcttcctcta
     7981 aaccaccagt acgcagcatg gcataaatca aggcacgcat gatttttaga tcactaggcg
     8041 ataagttagc agaagaaatt tgtccttctt ttttagatag catggcgttc atatcagatt
     8101 caaattgcga ataatctcca ccatacacat ctgctataaa ttccatcgtt tgcatagtaa
     8161 atgaaatagg gaattctgcg ccttgaataa ttacaacagc agaatttttt aagtcttcaa
     8221 cgtgaattcc ataatcagat aaccgagcca ttaacccgca cctccaactt tagccaatgt
     8281 tttccactgt tcttcatcgt atacaggttg agcaatgaat ttattaaata attctagaga
     8341 agctccgtct ctatttgaat caaaacttgc atacatcaca ttattgtatt tcagtccgat
     8401 ggaaaccaga tttgcagtta catcgtcaat ttttgtttct tcctctgcag tggcatactc
     8461 ttcgtcaatc acattggata gttgtgtatt aggataccaa actgcttttt taccgccatt
     8521 ttcaatattt ccaataaatc caaaggcgaa gtacggaaat tctcgtgcgg tatttttgct
     8581 aaacgttact ccgcttttag caactaatcc tttgatttca tccatcactt ctatcggaat
     8641 acccacgtga tccaacgcaa gttcatgttt tgtttctcgc gacacacgac gaaacatttt
     8701 gctggatgcc catttttcta atgctgttcc attccctttg acaccgattt ttgtggcaat
     8761 tggcaatcgt aaaacttcac tataagtcgg tgcagttcca acagaatctg gcgttgccat
     8821 catcgcaatt aaaatgtcgt ccaatccttc aaaataaaaa gtatcttgtt ttcccaatta
     8881 aaatcaccct tcccataaat caagtatttt tctagtcata atttcttcaa ttttttcttt
     8941 gttttgttca tacgtaccac ttgcaaaatg ttgggcacgt tgatttgttg tgccattttc
     9001 ggcaaagcgc cagtaaaagg ccgtctcttc aaattgaact ttcacttgat cgttttcaac
     9061 tactactttt atttgatcgc gcatatgttt cttttttaag agcgacttag gaatattagg
     9121 aagcaactgc tttagataaa agttagctgc ttcttctaaa gattccaacg ataatttttt
     9181 cggatcaact tttgaaaggt ttcctaaata atccgatatt tcagaaaaac cattcttatt
     9241 acttggcatt ttccacacac ctcacatatg tgtaatagtt ggttacggta tcgtcgttct
     9301 catcaccttg tatacctgta aaatctgaat atggaatgcc agcattttgc agcgcttgtt
     9361 caataacaac taaatcctgt tctgttccaa gtgtaaaaaa agagacttgg taataaggta
     9421 attttttata gactttaccg gatgccattt ttttgctgtt actcacattt gagtacacaa
     9481 tgtatggata catcgtccct aatctggctt tgtctctgaa cactgctaac tttgttgatt
     9541 tcagcgctgt tttcaattca tcaaagctaa tcgacataag ctaaactcaa ctccatttct
     9601 cttgcatcgg gattcgtata aatgcgagta atgttatacg ttacagaatc aattttgagc
     9661 gcacttaatt tctctgtgat ggatttatcc catctgactt taattcgtct gacaacgtct
     9721 gtcttggctt gctgtgataa atatttttct tgagaagtca caccgagttc ttcatagaat
     9781 attagacgct taaattcgta aattgtagtt ggacgatcgt ttccgtctgt tcctgttttg
     9841 atgtctagca attcggcttt ccatctgaga ttattggtct gtctcttcgg cattttgaat
     9901 cgctccttgc acgataaatg gcgtcatggc attcatagct ttgtcgagtt catcctctga
     9961 aactctatat tcataggcaa tgccggcaac catcaaaata agatattctt gttggccacc
    10021 agttgctgtt ttgacataat cttttgccat atttaaataa aaagagagca aagaatcatc
    10081 catgccctct tcaaaatgaa tatgtgattt gaatttttcc tctaaagaca attctttagt
    10141 ttgctcttcc atcttaacca ccaactggtt ttgtaatttc gtagcgatat actgccggtt
    10201 caaatggaga ataaaccaat tgaccatcta gcaagttgta aatttggaat ccaatttgat
    10261 ttttaccaga gaatttttca acaagttttt gaatttccaa ggcaccaata acttcttgaa
    10321 ttttaaatgc agaaaaatcg ccaaaatata aaactggcgt gtctggttca ccctttttat
    10381 ctgctgcatc tgtccaatcc acaggatagc caactaattg gtaaccaatt ccaccttctg
    10441 cttgtgtgaa tggccgcaac aaaggaaatc catcatctgt tttcattttt tcaatggcag
    10501 tcaaagcagc tcgattaata ataaagcgtc cctttttcat cacttctgtc actggtgtat
    10561 ttttaaattc gattaatgca tcatataatt tttgcccagc acctgaagca gttagatcta
    10621 aaggtttttt aaatgctaca gcctttttgg ctaatgcacc aggattttca tttccagcgt
    10681 catcaccatt gaacatataa ttgatttctt tacgcacata agcttttttc aattcttcca
    10741 caacaatatc ttcaactgga acaccagaca tttttaataa ttttttagtt actgttgcca
    10801 aagcatcgaa ttcggcagga ttaagcaaaa tttcatcaaa ctgaatagct gtttcagcaa
    10861 tatcagttga acgctctttc ttgtttacat tcgcatctgc tttcttcaca agaattggat
    10921 atttgacatc tcctgatgtt cgcaccactg ttccgtattt acgaagtaaa ttttcttctt
    10981 gagcataagt aataacttca gatgcaatta cttctgggac agtaactgaa ccgttgccag
    11041 cttcaatccc taaagctcga gcttctgctt cagaaatatt tccagctaca aaattagcaa
    11101 atgcttttcg tagttgttga tctttttgtt tcttggtcat ttttgcacgt gcctccaatc
    11161 cgttcttaat tgatccaagt aatccatctc gttgctgttg agtaatcatt ccagaacgat
    11221 tttctgaact atcttcactg tctgaatctt cttcttgatc gttgtcttca ccagaacggc
    11281 tttcgtctga atccgtacta ttcgattgat catctgtatt gtcgtctgtt tcatcagttc
    11341 cagaatctgc gcctaattcg tctttaattc cgttcaattc atcaataaca ctgtcaattt
    11401 cttctttcac ggcttctaaa tctgcttcgc gtaattctcc tgattcaact ttttcacgta
    11461 attcagtcaa tcgttgctca cgacgtgcct tcattttttt caataattct ttatccatgt
    11521 atttttcctc ctacgcttct agcgcttgat taattttttt gattaatttt tttctttctt
    11581 cgacgttttt ttctaattca tcacgacttc ttaatgcagc ttctgtatct tcgtatgctg
    11641 gcaaaggcac aatagaaacc tcgtataatt ccacttcatg gattgttcga agcattggtt
    11701 cagaattgta gtcccacgtt tcttcagtcg gcacaaaacc aaaactacat tggttgatgt
    11761 ctcctcgttc catagattta actaagtccc ttgctaccgt tgtgtccggt aaatcaactt
    11821 cgaatttcaa accacgttca tcttcttcaa ggcgaagggt tccgctttta gtacgtccta
    11881 gcacattgga ccaatcgtga ttgaacaaac aacgaacatc cgaattacta attgcctttg
    11941 agaatgcacc aggaacaatc acttcgctta agtcatccca taaaagtgtt tgactgttga
    12001 atacagcagc gtagccgcta atggtcctcg tatgagtttc ttcatcggaa cgtgttgaaa
    12061 gattggtgat gtcaatcgtg cgaatttcct tcttcttcat ttccatcacc tcctttcaag
    12121 tcttgatcat tcgttggtaa ggaatcatct gttgcatttt tctggccaat cctagataag
    12181 tcatttgaaa tatagatagc ttgtgtttct ggagtattct gtttaggaaa accaagcatt
    12241 tctgccacgt tatctggact tgtaatcccg gtacgaacga tgttgtagcc aatatttgtt
    12301 tttgttgagt aaggaacaaa atccaaaata ttaattttcc attccactct atagccagaa
    12361 ttaggcataa aaaaaagagc ggtgtaatgt tcgctcttgt ttttcaatat tggtttgatt
    12421 gctttgttgt gcagatacat catcgctttt tcaatatctg tcttcatcag cgattgatac
    12481 gtatcaacat tgattcctag aaattttcct aagtcttttt tgtaaacacc taaataattc
    12541 aaaattgccg cgtcatcaac aggacttttt aatgtctcga tggaatatcc ttttcccaaa
    12601 ggaatcatct taacagaatg atcactctca ttttgcgttc cttccagttg atccaatata
    12661 gctttgacaa tttttgtttg ggcgctatta tttggattga tgtgggcgtc tagtttaagc
    12721 atgaacgcaa gtaaaccgcc tttagtatat ttgtccgtca aaactttttc agcgctcaga
    12781 acgccttcta gagtgtttct tgcaagatca attattccag caccttttaa tgaatcagtt
    12841 ccgatgttct taatgtgtcg aatcatttga ccaggtattt tttgaccatt catttcaaat
    12901 tcttcttgaa gtcgttcatt gattttagtt gttacaccgt acgccaaatg aagctgatcc
    12961 ctatccgtta atgggaatgt ctcaccattg atcaataaag tatttgtttc caatttggta
    13021 aattcgaatc ctgtcaaata atcattggga ctcttcaaaa tatttagtaa gtgatgatct
    13081 ttgacttcgt tgccgtcggg accaataaca attggtgtgg ccaatgccac ctgattagat
    13141 atgtcttgaa ctaattcata aacatcagaa gattccatga tagatgaatc cgtcacatat
    13201 ctttgaccat aacgtgtgta gtgtccaaaa atatcctcga tatatccacg tttttccata
    13261 aaagaataga ctgcattcga taaccgatca cgtaatttca atttttttca ccgcctttct
    13321 atttatctat agatggaact taggtaatca tctaactcat ctgaatcaat atctgtcatt
    13381 tgattcatcg tttccttatg accacacaaa aacgccacga acccatcaat ctttttcttt
    13441 gattgacgtt tacttggcgc tttttgtcca ttgatgttag taattgctac aacgttcaaa
    13501 gtgcaataaa ggaataatgg attatcaaat tgaattcgtt tctcataaaa caaccgttcg
    13561 acatcgtcaa aaggagcatt caatacttta ggatattgag caacttcaac gcattccaat
    13621 cctaagtttt ctaatttctc aacaagtttg tcactcatcg ctggatcata attcacttgc
    13681 tgaatatcat ataaattcat gcagtcctct atgtactgca aaatctgatc ttgatcaatc
    13741 atttttccgt cgcaaaattc aacgaaacct tgttcagcca aatcactgta aggaacatta
    13801 tcttcttttt ctcgaaactc taaatcttca ttcggaataa aataaagttg ctttacttta
    13861 agaaccgctt taccatcttc atcccatgtt gggaagttta aagatacaca ggtcaaatct
    13921 cgtgtacgtg ataaatctaa accgatataa catggttcgc cacttaaatc accaagttct
    13981 tgtgtggtaa ctaaacatgg ctctacttga tcctgttcaa agaaattatc cgcgccgttc
    14041 acaaatacat ccaagtgttt cgttagaaat tcagctttgg aatgagctga ccgttgcgct
    14101 gttttaaatg ccgattctaa agcagataaa tcgactgata ttccccagtt aggattgcac
    14161 atttcccaga cttttctatc cgtccaatca taatttttat ttggctcata gattagaaca
    14221 aagtttgaat cattatcatc acgtttcaac acttcttttg cttcgcgata tacacgcatt
    14281 ccaacagacg acgaaccttt cccagcagtt gagatattaa acatcaatgg ctgtggtaaa
    14341 gatatctgag cagacttaaa attgtcatac tgttccattt tctcctgttt atgcaattca
    14401 tcgttcaata caaaatatgg attggatccc tcgatattat caatattctt ggtttgaaca
    14461 ataaatttat ttgagtaagc catatcttca tgaatatagt cataagtaat actagaaaca
    14521 gttccctttg gccccctaaa tattttagtt ccttcaagta ggattggatt gtttaagatt
    14581 gtaactgcaa aaggtttggc agcatattgc gcttgagcaa aatcagatgc gcaagcatag
    14641 caatcaactg ataacgcccc ttctccgtac attgcatatc ctaatgaacc gacagcaatc
    14701 aatgttttac cattcttttt tggaatctgg acataagcct cacgagtaac gcgtacaatt
    14761 tggccttttt cattttcttt aacccaacca taaatccaag aataaataaa tttttcccag
    14821 ggttctaaaa gaaacggttt tcccaccatt tctccttttg tgtgaacaat aaatgattct
    14881 acccaatcca tcatttcatt cgcgcgatct acatcaaacc aaatatcttt tctttttttc
    14941 cactgatacc agcgatctat tgctaaacga acggtcttag gatacttctt aggtttcttt
    15001 cttacttctt ttgcaaataa atcagcataa tttacaccag gttcaatcat ttttcagcac
    15061 ctgccttctt acgccattta tttctgtgcg ctgctagttc atctactggc tttacttctg
    15121 ggcgtgtaat ttcttcatct tttcttgcgg tcgatcctcc agcaattagt ctaccagttt
    15181 ttgctttgtt tgttaatcct aataaatcta gagctttcat cttcttatct gcccaagttt
    15241 ctacttgttg tgccaatgga tgttttgatt gattagtagc tcctgattta ttcgtgaatt
    15301 tttgcgtctc cggaaatcct ttttccttcc acaataaata tttgtgttgg taaatttcaa
    15361 aaatatccaa gtatgattcg attaatggat caagagtgat ggtgtacaaa tcagacaaat
    15421 tcattatttc taagatacga gctttttcag cacttacttt ttcatcaata atcgctttac
    15481 gttgcgcttt agtcgtcatt tttgtataca cccccctttg ttttttgaaa aatttgacct
    15541 aacgatgcgc gtgactcccc gctaccctat ctccccacgc gaaaaaattt tgaaaatgaa
    15601 taggggggct tcaattgaaa tacgaaggaa aaactttttt gtcttccgtt tcgttttcaa
    15661 caattggatg acattttgaa cataatagca tgagattgtt tggatcaagc ttaagcagtt
    15721 cgttgtcttt gattggtaca atgtgatgga cgtgtgctct ctttccaaat atgaactgac
    15781 cacaccgctg acaatgacca ccttctcttt cataaataaa ttgacgcata tctttccatg
    15841 cttgcgttcg atagaatggt ttgttctcat gatgataaac agactttgct tgctgcttct
    15901 tcttgcgtga tctgcttgat ctcttgtgtt cagtacagta gatacccttt gctattttgt
    15961 tcgtgcatcc gtcaaactga cagtatttca ttctacttca cgaataagat ttatgatatc
    16021 gccttttgca cggatagcac caggaatatc aataccatgt ttcttagcat atgcacgcaa
    16081 ttcttttgca gtcatattgt ccagttcatc cgtatcttca gtggactgat cattagtagc
    16141 ttcgtctcca tcaaaattag cagcagtgtt tccatcgcta tcaagaattt cactgctatg
    16201 agctatgagt tcgccattga cagcaacaaa tgatttgcca tcactcaaaa ttcctttttc
    16261 ttcttttact tcaaaatttg gttcttgatc tttcggaaca actactgtcc ttttcttttc
    16321 tgaatcccaa tactctgttc cactaatgga tgttctaatt ttgatcattg ccattttaat
    16381 tctctccttt gtaatttgta cggattactt ttgcgcccat tcgctcatac cattcaatcg
    16441 tttcctgcag attgggtaga gtgtgtgaca ataaagaaat aactaacgta ttcattccag
    16501 ttctgtaatc atcattctcc acttgcacat gtgcatgatt accattccaa ttttctctgt
    16561 acggactatt ctctgcttca attgctttct tataaacttt agccaaactt gattctactt
    16621 caatactaag taccgcttca tgaaaatcat tcatttgttt actctcctat cagatatttt
    16681 gtggtatgat ttataaaaaa gatgcgaggt gttttaatgc ttcaagaagt taatattaag
    16741 tatcgtgatc gtcaaaatac agagacaatt tatttgccaa atagttgtcc gcattgcgga
    16801 aatataatga ctccgtatgt gtattttggg gtttcatcat atagtgcgtt cgataatgat
    16861 agaatctttg gcgtattagt acagtgtata aatgatgagt gcaacaaatt ttatagttta
    16921 caatttataa atacgtttaa tgaagatgaa ggatattctg aaaatatttt aattaaagat
    16981 tattaccgtc cgccgattaa aatagattta ccagaaaata ttgaaaaagt ttctcctaca
    17041 tttgttgata tttatactca agccacacaa gctaaatctg agcaattgga acaaattgct
    17101 ggagttggtt atagaaaagc tctagaattt ctaattaaag attatgcaat atctaaaaat
    17161 tcagaggatg aagataaaat aaaaaaaatg ttattaggta aaacaattga aaattatttg
    17221 aatgattttc ctaaactcca aagattagcc aaagctgcta cttggattgg taatgatgaa
    17281 acgcactatg ttcgtagaca tactaataaa gatgttcatg atatggatgc atttattaaa
    17341 tcagcagcgc aatttatcgc ggctgattat gatgctgatt tagctgatca atttataaat
    17401 ggaaataact ttatgccaac tactaattaa aagaataaaa gagccacaga taccaattaa
    17461 taaaacggtt gggatatctt ggctctttca tatttttttg acactaatag aatatcatgc
    17521 tgctaacaat gtttacatag ctacaatgac cgaacattta gtgaacagtg taattttaga
    17581 ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg ttttcttttt
    17641 tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc tttctaattt
    17701 ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca atatcttcta
    17761 agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca gaaaagctat
    17821 ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca ttttctatct
    17881 tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg acgtttgcta
    17941 aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc cactttaagt
    18001 aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc acaaacgcca
    18061 ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt tttatttttt
    18121 cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct tcttaatctt
    18181 tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc gatttttaat
    18241 actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt ttgaataaga
    18301 ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat tacgttcacc
    18361 ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac cgttttcttt
    18421 tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt ttccggaacc
    18481 gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca tttaattcac
    18541 ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa tataagtgat
    18601 ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc caggtaattc
    18661 atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt tgaagtcacg
    18721 atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa aatattctgg
    18781 tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc atcatcaaaa
    18841 agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa gtcaaccaaa
    18901 tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt gtaaacatta
    18961 ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc tcctcttttt
    19021 gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt gatataatac
    19081 ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat gaatatcaac
    19141 aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca attaaaaaaa
    19201 gataattctc cagaattatt ttctaatttt accgaagacg acattcctac tcttaaacaa
    19261 ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa tttagaaaaa
    19321 ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta gtttcaactt
    19381 ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt tagcaacaaa
    19441 tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag ccattggttg
    19501 gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc tgcaagttga
    19561 atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag caatccgaca
    19621 tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc ttgagaaaag
    19681 agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc ctcaaggata
    19741 attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc ttatgcttgg
    19801 accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga tcaattaaag
    19861 cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct tcatctaaac
    19921 agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt tctccacgct
    19981 cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc agaatctgtt
    20041 actgccggga taatttaata ttcttttcat aattcacctt ttttcctact gacatcgaat
    20101 atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta cacatgatgt
    20161 ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt tcttgacgca
    20221 aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg gatcacttca
    20281 aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct tacagcaatg
    20341 gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt tagcggacta
    20401 cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt aggtagttgt
    20461 ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat tccaaaaatt
    20521 ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta atagtgcaaa
    20581 gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca tactccaatt
    20641 cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt agaacatatg
    20701 cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca atcatgtcta
    20761 tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg gacttaacga
    20821 aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca aaatccaagc
    20881 actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact actacatcgc
    20941 caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca acttccccga
    21001 tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat cccttatctt
    21061 ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca catacctaaa
    21121 ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa tatttttgct
    21181 cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc gatcaccatg
    21241 ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca ttgatttacc
    21301 gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg aataagtcaa
    21361 ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct ttcagtaatt
    21421 tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc cacttgtctt
    21481 cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt gctgtaaatg
    21541 tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt gcaagccttt
    21601 aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg ttccatcgat
    21661 atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct taattccttc
    21721 aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt cctcgccctt
    21781 tttataattc gctatttcat taattagttc tagataatcg atcttcattt ttccacctct
    21841 tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt gagataggct
    21901 tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt gtatccactg
    21961 cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt tgccaaatag
    22021 tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat tcgctcttgg
    22081 taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa aagaacattt
    22141 ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga aggcggtaac
    22201 tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt attatctttg
    22261 actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt aattcgtagg
    22321 tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt caagtatcca
    22381 aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc atataaatct
    22441 gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc cgttatctct
    22501 ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt ggctggtata
    22561 atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac tttttgattg
    22621 ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta atcacaccta
    22681 ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac tctgcatgat
    22741 caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg tcagctcctg
    22801 ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa tattcttgat
    22861 tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag atgaaatcta
    22921 agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg tctttcaggc
    22981 ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc cagaagtccg
    23041 ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag tttcgttgct
    23101 gtac
//
LOCUS       DASJTM010000045        23032 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJTM010000045 DASJTM010000000
VERSION     DASJTM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.045"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2563)
                     /locus_tag="U4B29_001623"
     CDS             complement(<1..2563)
                     /locus_tag="U4B29_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106574.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
     gene            complement(2785..3669)
                     /locus_tag="U4B29_001624"
     CDS             complement(2785..3669)
                     /locus_tag="U4B29_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2106575.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3764..4201)
                     /locus_tag="U4B29_001625"
     CDS             complement(3764..4201)
                     /locus_tag="U4B29_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106576.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4418..5329)
                     /locus_tag="U4B29_001626"
     CDS             complement(4418..5329)
                     /locus_tag="U4B29_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2106577.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5706..6344)
                     /locus_tag="U4B29_001627"
     CDS             complement(5706..6344)
                     /locus_tag="U4B29_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106578.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6348..7118)
                     /locus_tag="U4B29_001628"
     CDS             complement(6348..7118)
                     /locus_tag="U4B29_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN2106579.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(7536..7952)
                     /locus_tag="U4B29_001629"
     CDS             complement(7536..7952)
                     /locus_tag="U4B29_001629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106580.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(8003..8384)
                     /locus_tag="U4B29_001630"
                     /pseudo
     CDS             complement(8003..8384)
                     /locus_tag="U4B29_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8510..9175
                     /locus_tag="U4B29_001631"
     CDS             8510..9175
                     /locus_tag="U4B29_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106581.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="U4B29_001632"
     CDS             complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="U4B29_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN2106582.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="U4B29_001633"
     CDS             complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="U4B29_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN2106583.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="U4B29_001634"
     CDS             complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="U4B29_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN2106584.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="U4B29_001635"
     CDS             complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="U4B29_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN2106585.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21753..22328)
                     /locus_tag="U4B29_001636"
     CDS             complement(21753..22328)
                     /locus_tag="U4B29_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN2106586.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22328..22927)
                     /locus_tag="U4B29_001637"
     CDS             complement(22328..22927)
                     /locus_tag="U4B29_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN2106587.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4531 c         3342 g         8166 t
ORIGIN      
        1 ggatagaaag aacaaattta cctgtatcat caatagttgt ttcggacaaa acagcttgat
       61 tttttttcaa tgaaaccaaa gctcctgctt cgcctgtacc tgtgattgag cgagaagctg
      121 cattgatcaa tgtggtactt aatttagcag gctcaggagg aatcacatcg actgtcgttt
      181 tggcagagtc cagttctgtc acttcttggc cgcctcttac ttttttagca gcaactattt
      241 gcacttgatc ccccgttgtt agtctttctt cgttgggtac ttgaacctca aacaaaccgc
      301 cttgttcgcc ttctccccac ggattatatc ctgtcccttc attttcatct gtttgtcctt
      361 gtgtgagagc agtaatttct acaggtgatt tcgtcgtatc tgcataagta atacgtaaag
      421 tcacttctac ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa
      481 cgacatgccc atagattttt ttatctgcat tcgttggtac tcttaaggat tcaatcatcg
      541 gaagctggtt attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa
      601 attcatttgt taaacctgca ctagggcttg atacaaaatt acctaagtcg gttccattga
      661 tcgataagtc tgtcatatca gaaaaataat ccggatcttt ttcaaagtta tcgaacgtta
      721 ctcctgcctt tcgccataaa gcaatttctg aatgagcgat cttcaaagaa gaatcgccat
      781 ttccttgaaa tacccagcca cctatgccat tagtctcgct tccgatccgt ttgttttgga
      841 aatcgaaata ggtaggagat tctaagtcta cattcaacac atccccactg atgatgccag
      901 tcttatcttc cccagttttc ccgaccatga ctaaataagt tttttcgcca gccataaagt
      961 tcaaatcaat atctccagtt gtatctacgg tagctccgga atctgcgcgt atatcgatat
     1021 ttgatttttc tccagtaacc gtaaaatcag ctgaccctgg actttctaga aaaccagaag
     1081 cgtcataaaa ttggatcgct tggtttcctt gattgagtct gttcacataa gatttgccat
     1141 cccctgcatt tcttaccttc aatgagcctc cattagttac tgttagtttt tgcgtaccgt
     1201 tctcaaaacg aactgcgcta gaacgaccac tttgcttatc aatatcgatg actgatccgg
     1261 ctccttctac gatcaaagaa agtcctttcc ctacaaaacg catcccattg tttccaggtt
     1321 ggtcgccatc tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac
     1381 tagaatctcc ttgcaggcga aatccgccag tagtcgctgt attgattgtg attttcccat
     1441 cattcgacac agcgactctt gatcctgagc ctctcatttg gataattccg cgattgttgt
     1501 cgttggtaat attgatcttt gtatttaccg aaaattcact tcctgagcca ctgactttaa
     1561 atgtgacatt cccgggtagc gtatcgacag cctctccttc atcgctgacg ccatctgccc
     1621 gatcatagtc aagagagacc tttgaaccac tcaagatatg gagtcctggt gctgtttttc
     1681 cactgcccgt ctcatcacct gaatctagaa aaccatagaa ttttcctaga tatggtccat
     1741 aaagctgact accattgatt acatttagct gatagcttct acaaaaataa gctttacccg
     1801 gagcggcatt catcccttct ttcactggtt ggacatccaa tacaccggaa acaccatcta
     1861 atgtgacagt tgcacgaggg gcgtaaatca ggcctaattt agaaccatca tataagtcaa
     1921 gtgtcccggt aaattttatt tctcccgtaa aatgatatct gtttccccaa aaatcggaca
     1981 tataagcaat gataatggca tttccatcga tatttggtat caacccacct ggatgagctg
     2041 ccgttccttt aaaattctga aactcgatcg actgaggtgt ctcattgata tagatgtgat
     2101 gtgtgccgaa attatgagca agtccgccaa aatcgataac gatatctcgc ttgatctcca
     2161 attccctgtc taaagtcaaa ggctgagtta aacgaatctt agctactgat tcgtctgcga
     2221 gaagctgcgt cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacaactc
     2281 taacctgtac ctcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg
     2341 tcttattttc ttcttgcctt gtattagaag taggcgcctc actttgggat tctgtagttt
     2401 cagaagtaat tgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
     2461 ttgattcgct tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
     2521 caaacaaaat cgacgcaatc agctttttta catattgatt cattctccac catcctctct
     2581 gacgttttga caattttatt ttaacgtttt aatttatttg taattgtact ttcatattta
     2641 aaatatgtat ttttatggca aatttttctg ttaggtgaaa atttcttttt tgtacaaaaa
     2701 aaggctgtga caaaagtctt gtcacagtcc cttattccga acaaccgatg tcctcctgac
     2761 aactcttcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gtaacacttt
     2821 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     2881 atcagctaat gacacaacta attgctcaca agtaagatag gctagatttt tcgtagattc
     2941 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     3001 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     3061 accgcctact gagttagtta gtcctatcag caagatactg ctctcaattt cttccttctt
     3121 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     3181 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattgc
     3241 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     3301 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     3361 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     3421 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     3481 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     3541 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     3601 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     3661 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     3721 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     3781 aaaataccca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     3841 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     3901 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     3961 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacgacctt
     4021 gaataataaa tgcaacaatc cccgctataa ttagtgtgcc taataccaaa acggaaaatt
     4081 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     4141 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     4201 tttatctact tctacgtgca ttttttaata tcgttttctt tatttaagat tatacagaaa
     4261 aatatctagg gtggctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     4321 aagtaataat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
     4381 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
     4441 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
     4501 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
     4561 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
     4621 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
     4681 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
     4741 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
     4801 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
     4861 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
     4921 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
     4981 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
     5041 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgggaatcga
     5101 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
     5161 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
     5221 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
     5281 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
     5341 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
     5401 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     5461 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     5521 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     5581 aatgttatcg attttgtttt tcttcttccg ctgttgggat attcaaatca gacactttta
     5641 tgttcgattt tccagacatt tttttgttgt ttttattaga aaaagtttgt tttttcattt
     5701 gttttctaaa gcatgatctt cttgtttcct attttggtca gatatggatc atgcgtgacc
     5761 acgataacta acgtaccttg ggaagcctgt tgcgataaga tttcaacgac ttgctcctta
     5821 tttcgtttat ctaatgatcc agttggttca tcggctaaaa tgattttgct attcttcaat
     5881 aagactctcg cacaggcgat tctttgcttc tcaccaccag ataatgtgta gataggcgta
     5941 cttagcttct tgtttatatt tacttttttc aatgcctcat actttgctgt tcgcttatct
     6001 gaaacattta aattcgaaaa cttcaatcca atatctaaat taaaatcgat tgtttcatat
     6061 tcaatcaacc caaaatcttg aaaaagaaaa gagagggcat ttctttttat caagcgcttc
     6121 tctcttccag ttaaatgttt tgtttcttga ttaaataaat aaaattgccc tgaactgaat
     6181 tgatcattca atcctaatat attcaataaa gtacttttcc ctgctccgct ctctccaact
     6241 acagtcacaa aatccccagc atccgcagtg aaacgatcca aatgaagaat cgttttgttt
     6301 cgaatgattt ttttcgcacc ttttatcact atcaacggct ccaattgtta acctccattc
     6361 atcagcaata actgactttt cttttctgta gacaaaataa aaacaactga aagaataaaa
     6421 tcaatgaagc taaagaaaaa aatattaaaa agatatttag gctctctttc tataagaaaa
     6481 aggaagatta cacttgtcgt tccccatttg atgaggttca tcatgaagaa atctttatac
     6541 gtttcaaaga gcgaataacc acttgaacga aagatagcga ttttccgaca attgtagaca
     6601 aaatatatgc ataacatgac atagctcaac aagaagaaag aaaagaaagt gagtaaaaat
     6661 atctgtatca agtaatgaaa tgatctgctt aacactttga cagctatctc ttcagtctga
     6721 gtatacggaa atgataactg caagttatca cgtaactgac tttcttcaat cagctcaaat
     6781 aacctttctt tcgacaaaga gcctatcttg atctttaacg gtggatagat ttctccgctg
     6841 agaatatttc tttcccaata cgtactgttt ttcaatgtca gtatttcaac aaccggataa
     6901 tgttcgatcc aggggataga aggaataaat gtataaatgg gttgattgtt ttttataaat
     6961 agaaatttcg gttctttttc actgatttct tgataatatt ctgttatttg cgccaggtca
     7021 ttttttaaat ctgtatgtct atatcgttca gggatcaata taacagggtg cgtttctttt
     7081 tcggttattt ggatcaacct tccagagaca tcaatcaaag gaaatttctt aagataattc
     7141 agatttattt ttatccctct gccaaactgt tcattttctt cattcaagta atctgataca
     7201 ttgactaata tacttccgtt tttattcaaa gtttcatata attcctgatc ggctgctgat
     7261 gcaaaacgat tggaataaat aaaatccacg tggtttttcc cgatggttac tggataaaat
     7321 acatgatagt cctcaccagc gatttctttt ggtgtttcat gggaaacagc taactgtgaa
     7381 gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg tcaaaagaag agaaaacaac
     7441 aatccaaatg taaaaagaat gtattggata tagttgaacg cttctcttaa cgaagcgatt
     7501 ttttcaacta ataaaagaga aatcaaagag ataccctata ttccgctgaa caagaaaacg
     7561 tacatctgga acaaataaag aaattgctcc ttataataca taaaaataaa accaccaatg
     7621 attcctgcaa ttatgaatgt aataatgatt ttctttttcc ccaataaaca ccacaatact
     7681 tgatgtaatg aaaagccatt attcaacaat aaactgattt ctttattcat caataagaaa
     7741 aaggatgata acaaaaggaa taaaagaatc aatccaatag ttaaatacac tcgataatta
     7801 tatacgtctg tacttgaaat aaccgaatac gaatagctat tttcaaagtc ttcaaaaata
     7861 ttagtggcat aatcagtgtg aaatgcttga ttgaatcgtt ccacaaatag taaaatgaac
     7921 cgttcatagg cttctttttc tctcgtcatc aagaaaaatt caccttcaaa atcttttatt
     7981 ttgatcgatt cttcaatgga cgttacgcga aaggaggtat tagaataggg taccttctcg
     8041 tgagaagatt tgacatcccc ttgataatac aatgtcattt caagctttgg ctgtatcttg
     8101 aagttccaag atgtatctac ttcattggat tcatttactg tttttttcat ataagaaagc
     8161 tcagtttctg atgagacatt ttcaaatacc atgattagct gctcatattc ttcttgccca
     8221 ataaaatgtt gtggcaatcg aatcggagta taagtatctg tttgatggaa aaattcattt
     8281 gtccgataga gtacctgttt atttataata accaaggcaa aaccactcat gataccggtc
     8341 actatgattg tgagtaaaac tgtgaacttt tttttgatta tcaccactct cccttactta
     8401 aatgattctt tgataaaatc ataccgtttt tcatcaaaaa aacgtatcta tcaaatgtat
     8461 aggtcatgta gaataaatct atatactgta gaatcgaaag gagaagaaga tgaacatcgg
     8521 agaaaaaata cgaacttacc gcagaaattg taagcttacg caaaaagaat taggcgaaaa
     8581 aatcggtgtt tccgataaaa caatctctag ttgggaaaat tctcgtacaa tgccagacct
     8641 tgaaatgctg tcacttttac atcagacttt aggtctgccg attgattttc cttctattga
     8701 atcagctacc gaatcaatag aaccaacatt gccggatgaa cctcttgtta tcaagaaatc
     8761 ttattcatca aaacaaccct tctcactcaa aaagacacta tctttctttc tgctcgtctt
     8821 ttgtttggtc tatctttttc ttatcaatta tcaatataaa aatgaaacgg ttgatcgatt
     8881 caatcctttt ttgaaaccac aaatcggcta taccacactc cctgtaggat acgtggaaaa
     8941 ttccatcgaa cattgggtat gtaatgatat ctttgttcat ggtacatggc tgtcttttca
     9001 cgctggagag gtagaaaaag gtaagaatca tgctgtaatc aaacacaaag gaaaacacgt
     9061 catgaacgtt cgcctgctca cttgggaagc cattccctca gattaccaag aaatcctcat
     9121 ccaagactat gacgatgcct taaaaagaag ccctcaggag ccagataggc attaagtagg
     9181 agatgaacgt agagatagaa aaaatcaatg cacatgatct tatcaaaaaa aaacaggggt
     9241 acaaattaat tgaaaaagct ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
     9301 ggtaaaaaaa tgactaacta ttctagtatt aggctatttg tattattctt ctgttatttt
     9361 tatatcaaaa aatccagaaa taactaaata taggtatttc tggcttatag tataatattt
     9421 acgccacagc ccccaatgat tctttttgga atcatgatca aggacggaca tacacaaaaa
     9481 tattttgccc attcgatccg aatagattaa cgactaatct agtaagtaat atagattggt
     9541 acgtagtcaa accagcaaat ggaaaagttg tctattccac cccaagggct ttaaagacag
     9601 catcttccgg cgtttgataa aaaatcaaat taaaggtacc cattaattct gggggtacag
     9661 ttccaatatc agcagcgctt atcataggta caagtacttt tttcgctccg gcatcttttg
     9721 ctacttgtaa acagttagct aaattatcta ccttaatcat agaaccacca attgtaatat
     9781 cccctaaaat aactaaactt ggtaatacag gtttatttaa ggaaactgag cataaagcta
     9841 ttatagttga taacgataat gtttttgtca taccaatgcc attcatatct tgaacatgaa
     9901 cgacaaaatc ttttgtcgtc aaactaatag actgactaac acttccataa ttagctctta
     9961 aatatcggta tgccgaatcc atcgcttctc ttgctccagc atctgaattg atcccagtac
    10021 gttcaaactt tccatttccc gaagtcattt gcgtttccaa tttataggta cctaattgcc
    10081 cagttttgcc ataagatacc gtgtacaaat gccctggctt ccctaaccct tctggaatca
    10141 aagttcctcc accttgttca ggaacagaaa catactcttc tcgctcttct tctaaatcaa
    10201 tgtatgaaaa attgacatca taaaattcca ttccaccaat tttctttaat tgttctttta
    10261 cacgacgacg catttcaaga gcaaatctta gtatttcttc gacatcactt ttttcaaatc
    10321 taccatcagg atacaacagt ttcatcatcc cagatacaat ccgacgaact gcaatcgtat
    10381 cccgctgatt taattgagaa ccaagtttga aatatttatc aaaactatcg ccaaaactaa
    10441 cttttcgaag ctcacgcatg acttcagaaa aataatctgt aatgaagcca tagtcatttg
    10501 taaaaaaatc tggtcgatat ttaggaattt cccaacctgg taaataacaa tgcatccgat
    10561 ccaaaaaggc tgaatccgtc cccataacct ccggaaatgg ctcaaataaa ttcgaagttt
    10621 tcaatattac atcaacgctt tgattaatat ttcctacaaa agccattgat gctgaagcat
    10681 tcttttcttc tttgcctcta gcaaaagatc ctgaagccat gtaatctttc atgatttgaa
    10741 cgccatcatt atccttaaat ttaatcccag caacttcgtc aaaagcaaca cagtcccaca
    10801 tacctacaag tcccattgta cgactagcca tattatagaa aaggtttgct actgttgttt
    10861 gtccacctga aacaaggatt gagtttggtg aaatttcttt atatacatgc gatttacctg
    10921 taccacgtgg tcctagttca cagagattaa aattattttc aattaatgga ataagtcttg
    10981 ctaaatgaag ccattttacc cttttattaa attgtgtagg ttccatacca atggaccgta
    11041 gaagtaagtc aatccattca tttgtagtaa actccttgcg agccatcttg taagcctcaa
    11101 tgtctaaacc aggcatttgg attggatcaa ccttttttgc aataaaaggg ctattccaac
    11161 gatcattctc atcataccaa tattccatcg aaacaataca ccagatcccc ccacctaata
    11221 atcgatcaaa ttttttcgga tactcttcac taatttgaac attttttaat cccaggttag
    11281 agaattctgc ttcataacaa tctttctttt catttaaatg tacagttact ttatcaataa
    11341 ctgtataacc acccaattca cgaatttttg cttttacttt ttctgcttca tcaggacgca
    11401 catagtttcg agcaagaata tttttaacat tttccacacc tttttcaacc aatgtttcat
    11461 ccaatgacga agcatacata ccaagtaaat attccaatac ataaacaggt acattcgcac
    11521 cttctttaat tttctttgtt aaatcttttc tgacaatctt tccgggaaag aattcagata
    11581 actttaaaat ttcctgttgt tccactagaa ttcctccatg tgttcagtca ttataatagc
    11641 aattaaagta tatcatactt aaccagtagg cttgataata caaaagaaaa agtatccaca
    11701 aaaaattgtg gatacctcgt ttttatatat caaaatcaaa tgattctgca aaaggattat
    11761 ctataatgaa ttttattcgc tcggtcaaat catttttttg attttttatc actaaataat
    11821 aagtatcttc aactgtatat cgacgattta caaattcaaa aacaaattta gtaaaccgct
    11881 gatttgcatc ttctgttttt gaatccgcaa catagatttc ttcatttgag atacgttcac
    11941 catattcatc ttcaaaataa agccagaaag atgcggggtc ataaacatca ctaattgcat
    12001 ttttttgata gaattctaac gtggttgata atcctgtaat tctgcggttg ctagtcatta
    12061 attcaattgc tactggttcc tttgcattgc tacctttggc taccgtaatt tctagaacag
    12121 ggactagcat ttcttgaata gatgaccctc catgaacgta gctttgtccc ccgccagctt
    12181 tcttaaaaat acttgaagta atcgggacac aaatgtttct agtgtcatca tttcctaaag
    12241 aatctcccaa agagtaattt ttcgtaccaa ttatttcgta ttcttcttca ctaagaataa
    12301 aacgacgttc tacacgatct ttttcatttc tagatggatt ttcaatctta tctacttctt
    12361 caacgtggct tcttttataa ataaatccat gatcagccgt aactataaaa cgatagatac
    12421 tgccatcatt atgcaagcga ttaataagtc gggaaatttc gcttatcgct aaatcacagg
    12481 cctcaaaaac tcgattttca ttcgagtgat cacctgttct atccacttga tcgtgataaa
    12541 tataaatgac tgatttgcca ttaaatactt ctcgtacctc agccttattc atcttaacca
    12601 catcatcata atttaaagct acagcatttg catctcgttg ctggagaatc gcatttcgat
    12661 tggataaacc attcgttttt ttcccatcaa tcagtacttc tgttccttca ttgtaagtta
    12721 gttgattgtt tggtaataag gcggctttac cgaattcagt aactgaaggt aatacagaaa
    12781 gaaagctagc catgtttgtt gaatacttct tttctctaac taacttacgt tctaaattca
    12841 tccctaactc atatcgaaaa gcatctgaaa taataactac agttttaaca gagcgtttca
    12901 ttacagcttg attataaaat tttcgaatgg atggacgatt cttaaattca aaagtttgat
    12961 tccaaatttg tcctgattga tcaagaaact tcgaataatt tgcttcaact aatcgataca
    13021 attggccata tttttgcttt tcttctatat gatcactatt ccaaataaat ttccgataat
    13081 gcgtatcaac ccaatacatt tccttctcat attgagttag caaattatcc atgttcatcg
    13141 gcaagcacaa tctgttttta tccacaagat aataagcatg tttcaataat tgatactcat
    13201 aaatataatc cgatgcgaaa tgcatttttt cgcgctgctt taaactatca aaaattgttt
    13261 gtcctgcaat tttcgttgta gtgtcagaag cctttaatcg ttctgcataa aacattaata
    13321 tttttgcatg aatctcagaa aatacgtctg cttcaattag atcatcaatt tctatactgt
    13381 ctaataatcc atcaccatca atagcctgat atactttttt agatagctgt tcaaagcttt
    13441 ccatgtaacg tgtatcattc ataaactgat ccatgaaagt aataatgttt gctttcaaat
    13501 gtagtatttc aaatcctttg aacttcttcg gcatctcttg atcaatttga taataaaaat
    13561 agttcaaata aagacacatc gtcagctttt ccaatgttgg ctggctatcc gtaaagccaa
    13621 atttcatttg aataaattcc caaaatttag ctgttaactg atacttatca agttccctta
    13681 aaggattctc cctactttct gtaaagcttt ttagaactac acgcagtaaa tcatttaatc
    13741 gaatactttt cgatttcaac aaaacagcca ttatcattaa ttcaatcgtt tctgatttat
    13801 taattgtcat atgcgtacta aattcaccaa aacttctcat gcgatccttt gcattaaaga
    13861 aattacgata ctgtttaact gtctgatgaa atctaagtgg aatagccaat tcattcatga
    13921 taatcgctaa gcggtctgca ctaaattcct ctgaataatt caatacactc aacaaatgat
    13981 tatcttcatc tttatcatcc caacgaggaa atggcgcata gattaaatag ttagtttcgg
    14041 gatcttcata ttccaataaa cgcttagttt taaattgtgc gtccttttct aatcgataga
    14101 tttttgcatt ttgaatgacc aatgagtcaa tttcttcaag aaaatcctga tttgaatcaa
    14161 accaaaaaat gattttccta catccataat taggaaaacg ttgatttaat ttttcttcaa
    14221 tttgttgcgt atttatatct gtcatcaatc tcacctaatc gttctctttc tatacccctt
    14281 atattgacat aatttggata atttcgcacg caatttaaga ataaaaaaga aagttgttag
    14341 aaaacaactt tcttagataa ctattttttt ggttctttga ccccttttgc aaaaatttgg
    14401 aatttctcgc cggttttagg atcacgctgc aacttatcat aattgacaat tacaccatca
    14461 tctaagtcta attcaattcg ttttaatgct aagtgaccca acttctcgtc atactcacga
    14521 acttctttta attgcttctg aatctttaca atttcttttg tcattgcatt tctttctttt
    14581 gggttatcac ttgttgaaat ttgttgctga cgcaattcga ttcgattgtc atacgctttt
    14641 tgtagttcgt gtaggtattc agtacgtact tttcctaatt gatctggtgt atagcgatgc
    14701 aagtacatta acgctttaaa gccattttgt tttcctgagt ccaattgcca ataaattggt
    14761 cgattggagt atgtttttaa atggtctttg taaaaatctt gtacgaagta acgacgaata
    14821 atttctcgac ttgtcccttt gccacctaaa gtgtcagcga taaactggag attttcttca
    14881 agggtttctt taccaaaagt aatctcaacg aaatcaatga aacgattgac aatgtcaatt
    14941 tggctatcct tgaaatactc atcatcggtg atgagtaaaa cattatcagt atcaggttta
    15001 aatttttgat agcgggaagg atcccattcg ccgccagcga agatgagacc atcttcgtct
    15061 agggagtaac gaccgaagat acaacctatt gaataagaaa taaaatcctt aatttccctc
    15121 gccttatcag gtaaccttgc agagatatat tcatcttgaa cctcttttgt taattctttt
    15181 tcaagagaat aatgattaat gaatatgtcg ttcagttcct cttcattcga ttttagagta
    15241 ttaaattgat taaccaactg cctacttaga cagttaaatg cctctgagag atctttttta
    15301 aacatataac ttaacaaaat gtttcttcta aactcgtatg acgtttcaag agcattatag
    15361 tcttttctag ctatatcgat attttcatcc actttttctc ttattttttt taattttctc
    15421 tcagatgtaa ctttaagaaa aggtagtttt cccattggtc cttcatggta atccaaggtg
    15481 ggagaaataa aagttaaaaa gtgattaact aatttagagt ttacatagcc aaatatttct
    15541 tcaaagtcag ttatatcctt tgagaacaga acagatcctt tagtttcaaa aattgcctga
    15601 ttctgcaatc taaaagatac ttgtccagat gaaagtgtcg accaagtaat tcccttttta
    15661 aaatagtaat taagattttg tggtctagat tttaacttcc cattttcatt aacataattt
    15721 tttatttcat ctccatcatt ctcccagtta accacgtatt cattgtttcc ataccatttt
    15781 ctaaaacgac cacctttagt tgtaggaaac catttttttc tagacttttt agcgtcctct
    15841 aatgttttag catcaaactt tattttgtta aattctactt caaaccaata ccgcaaaaat
    15901 ttattgttat ttccggttgc caacccttgt tttgcatctg caacatcgtt agttaatttt
    15961 ccttgagcaa aatcagtgat tacgttatcg cttgcccagt aagcaatcgg cattcctggg
    16021 atttttttga agttgtcttg attagtacta taaaaataat cgcattcacc atgctgatga
    16081 attgcttcta acgttttaat tttttgaact tccattccac ctttaaaatc tgataatttt
    16141 atatagcttc catcttgttc atttgaattt tttatcgtaa acgtacaaat tggaacggtt
    16201 gcttcttcaa aagctgaata ctccatttga atcaaactag aaatgtttct ttcattaatt
    16261 aaaaatgagc gtaacttttc atagctagaa atgaacatcc aaacaaatgg cgtcataaag
    16321 gaagcatatc cattatcaat cagcatttgg ctattcaatt taataaacac agaaaataga
    16381 tcttttttga catcagaata gttatcattt acatattttt ttaatttctt atccatttta
    16441 ttaagataag gcggattagt gactaccgct acatatttat caactagcat ttcagccacg
    16501 tcaagaatgt gcaagtattt ttcttgtatt tccttcatcc cataaagaat atccatattc
    16561 gtaaattcgt tgaattttga tatatactct cttacttttc ctaagtattc tttagttatt
    16621 gcctgattat ttttatcgac taacttaatg attgatccaa gttctttccc attttcaaat
    16681 tgatcaaaaa ttaactcttt gtattcattc cagcgttcac tatacttatc attgttcaat
    16741 tcactaatac gctcttgata ctcgtctgtc actttaggag aatcaataaa ctctcttaaa
    16801 ttcggcgata gacttttttt caaaaaaaga cgatcgtatt gacgcccttt catcataatt
    16861 gcaaaataag acaattgata cgctctttta tcgatttcaa gaccatataa attgtattca
    16921 acgattgcac gtgcagcatc acgctcagaa tatcctaacg taagataaat ttccatcaat
    16981 acatcgaagg cgtaaactaa aatatgccca gaccccattg cgttatcgat aatttttagt
    17041 tcttctggtt ttaaattact tacagatgtt gcttgttcaa aagcatattt atctggaatt
    17101 aagtatttca acttatttct taattcacta ttgggataat gatctaacca atactttcct
    17161 aaactattgt caaccatata acgaacgacc caatctgtgg taaatagttg agttgctgcc
    17221 ggaatatctt gttttttgac aatcccttta taaatattca ccacttgatt atgtggctca
    17281 ctattatagt actgatacaa ccacccaata atctctacct gtccaccagc ttgaacatag
    17341 aaatcatcct ctggaatact taaaagatca gcgattacac catctttttt aatataagaa
    17401 gctgtaaaca aaagttctgt ataatcttct acttcttcaa atagctcagg taagtaatca
    17461 ttcaattgat tacagatttt tataaaaatc aattgataca gcttgtccat tgcttctgct
    17521 gaattatctg ttttccaagt tctgacttgt tgctgttcat tactagataa ttcttcaaag
    17581 atatcggcat cataaatatc tgtaataata tctggctcaa ttttcccact tatttcacta
    17641 gataatacac gatacgtgtc aggtaaataa tcatttacct ccataaaccg aatggcaatc
    17701 aatcggttaa accacgtata agcaacctct tcgattaact cttgaaaagc ttctgaataa
    17761 gaagtgacag aagcacgttg ttttagttct gcaatcaatt tgtttctttg cgctatttct
    17821 tttccttgaa ttcttattcc tgatttttcg atttcttgta aattgctatc aattttcttc
    17881 acatcttcaa tttttgattc atcaattcct aaacgttttg ctttagtcat tacttcatca
    17941 attaattttc tacgggcatt gaccgcgaaa ttctttatcg ctttcttatc catttttctt
    18001 tcccttctat atgtaacctt tgaaaagaca ggcacctttt tatgcctgtc ttttcactag
    18061 aattgaatcg tgatttctgt attttcttga agttgttcct tcagtttttg attaatttca
    18121 gctgttaact catcaatatc tgctgctgac ttaatagttt taacatgaac gttaagtgtt
    18181 ctcattggta ctgttttctg ttgaatagta ggttttacta tcgctatttc ttcagctttt
    18241 gctgcagagt ctatttcatc tttttcagtt tcacgaatca agtttgctgc ttcaatttgt
    18301 ttacgtgctt gttcttcttg acgtctgcgg ttttcttcat tatcgaaacg ttgattaaag
    18361 atcaaatact gttctgctac gtttaatgat aagaaaacaa gatcatctat agtattcgct
    18421 tgttcaattt ttgctttcag ttcttgaaat tcttggtcaa ctattggtac aaattgatcc
    18481 ttaaaatctt ttccttctaa taatttaaat gttgcttgtt tttcttccgt taatttattt
    18541 aatatttcct gactttccaa ctctaacagt tgattatatt gttcaataaa ttcttgaatc
    18601 aactcattta gtttcatcat atctgaataa ggatcagcct ttttcaaaat ggttttcatc
    18661 tcatgaatca attgatcaat ttcttggcta gaagtttgac gccgactttt ttcgattaat
    18721 ctaattttat cttgtgcatt ctcccagatt tttttctgag tactattttg cttgtggaaa
    18781 tttttaattt tatcatatgt ttcagcccag tcgaataatt catcgcgata ttttttgatt
    18841 gtataaaaaa agtcttcaac atctgacgtt tgaacaatta cttcaagata tttttttatt
    18901 tgctcatatt cataagcact agggtaggta taagtgtttt gtaaatcaaa ataagagcgt
    18961 aaattgtcta attgaccttt taaatatttc ttgaaatttt ccattgtatg ttcattgtta
    19021 ttttcatcaa caatattacg actttgaaac agttctctac cgatttcgtc aaccaatttt
    19081 tttgcttttg gcgaaatcac cgcgcttggt tcaattttta tcttttcact ttctcgtcgt
    19141 ttttgaataa tcattacaat actatttgga tcatccatcc gtgaaatttg tacattgtta
    19201 taaagtaaat caattttacc agacatgaaa agtttagcga tgcaccattc cacatcaagt
    19261 tctgtgaatc catacggttg ttttgtttca aaacgatcac gaattgtttt catcgtaatc
    19321 gttaggtgat ttctcttttg atcattgata aaattgaaaa tttcctgaac tgcttgctca
    19381 tttgatggat cttcaataat ttcatttttg ctttctttca ctaaatataa gatatcttct
    19441 ttttctttag gcgcatcaat ttggtttaat ttatggtata cttcttcagc cattttctga
    19501 agtgctgatt ggaattttgc tttaaagtcg gagcctgtca gttgaatttc tgcaccgtta
    19561 tagaaaaatt ttgcagattt caacagttca tttaattgat cgttgatact tgcacttagt
    19621 tcctgtaatt caccagattt ttccgaacga atcgcgtcaa agttctttac tacactcgaa
    19681 taagtcgttg ttaaataaag attcattcta gcatacattg ctatatcatc tttaaattct
    19741 ttatctgatg gcaattctac gaataataag ttacgggaac tcttaagcgc aagttctgcc
    19801 tcttgacctg ctaaatcaga gtatggtgaa ataaaattaa ctgtcatttc agcatttcca
    19861 gcttgcttgt atggacgttg atctaacact tgtgcataag taaacgtata ccggttttta
    19921 aatttatttc cacctgacat gcctaatttt tcataggcaa aactatttcg gtctagaacg
    19981 ccgtcaaaaa ttcgtttaga tagttctacg atcaaatcac tatctgtaat attttgattt
    20041 ttaatctcac gagtaatttc ctgttcttca tcagtttgga aaatataact atctaagttc
    20101 ttccgcacaa agttttcctt aattaaaagg tccaatgctt cttctactcg ttttttagtt
    20161 tccaggcggt cttcgttgat atttcttatc atcaagctcg taatattttc aacatttggt
    20221 ttgatggctt gatcaacata tttaatcatg aataaaattc gtaagacatt tacgttaaag
    20281 ttatctatat cagttggtct ttgaatatga gaattatttg tagcttgtgt aatcactcgg
    20341 ctaattgctg tatcaatcca acgttctaac gagtaataaa actgatctaa agaaaccaaa
    20401 ctggttgtat cattttcctt atttcgttca gcggcttctt taaacattgc tagtaatgaa
    20461 cgttcaccag acgataagtt tgatccttga tagctgtgat tacgaatctc ggttagaatt
    20521 tgccccaata aattgaattg atagggaata aatggataaa cttctgcgta attttcagca
    20581 tcttggaaga gctttaattc aggactatca acaaagagta atttattttt caattctgtt
    20641 tgaacagttt caaattgtaa ggctaaagaa tcattggcaa actctttttt cttcaaaata
    20701 cgttctttaa ttacttcatc ggcatttgct gaagataaac tcacagttgt ggcaaaacga
    20761 tccttgatct tagaaaaatc attatcagaa ttgttatcaa agtttgttac gtgatcaatc
    20821 gcttcctgac ttgtcactaa tacccaagcc ttaccttcac aaattcgacc caaatcttcc
    20881 acaacggttt gtaactgcaa agtcaactca cgattaccgc cgatatattg tccgacctca
    20941 tcgactagaa aggcaatatg atggtctgct tctttggaat caagatagct tttaactaga
    21001 tttgcaaagt catcaatgct tattaaataa tttcctgatg ctttttctaa aaaattatca
    21061 atagcggctt cactcataaa atcgatttta agtgccgctt ctttgatatc atcttggatc
    21121 caatcaaact catggcgctc ttcttgccat tttgccccat taatctcttc aaaagcagct
    21181 ttgaatgcat caaacttttc ttctttaatt aaccgacgtt caaaatctgc tagatgtgga
    21241 atcgtgctta atccttgcat ttcgttaaat acctttaaaa aaacattcac gattccgttt
    21301 ttgtcttttt taccacctgc atcacttttt gaatcaatat taaacagaat aacatctgta
    21361 tttatttgtg agcaccgttc catcgttgcc aaagtcagtg gatctttgat tttcccatct
    21421 tctttgaagt aatcaatggc tttcttgtca tttatttctc gatttgcgag caagtaagaa
    21481 ataattttta agaaatgaga tttacctgaa ccaaagaacc cctgaatcca aacacccatt
    21541 ttatctgttg gtgtattgat actccgttgg taggcatcaa aaattgtttt aaagtgtttt
    21601 tgtagttctt tcgtaaccac gtattcttct aattcttgtt gaacattttc atcctttgct
    21661 tgaccgacct taataactcc ttgaatattt cggtcaattg gttttgcaaa aatatcttta
    21721 attagcattt aatctcctac cttccatccc aactaaattc taaatgctcg ataataatta
    21781 tcatctttat tggtattaag aattgtcaaa cttaattcat catattcccc tggatacatc
    21841 atcacaactg gtacgcgatc aaatacctga tgcattttat ttaaaataga gtgcgctcta
    21901 attaatggat ataattcgcc aattcctgtc aggaacacaa ctgatttttc tggtgtatgg
    21961 gcatccacat attcaaaaaa tacattttct tcctcaggta aatccatttt caataaacga
    22021 ccaatgcttt ccactaattt ttgtaaacca cgtcgttctt ccatcttaat cgctttttca
    22081 gtgaaaccgc gtttttctag atactctaac ataatcacat agagattata aactttcact
    22141 tcataattat caccaacaga tgttccagat atatcattaa tataatctct tacaattaac
    22201 tcgtctttcg gatcgtagtc aaagatataa tagcctactt cattacctaa accacgattc
    22261 aataaaaaat cttctttttg aattacttca ttcaactttt ccaaatgctc ttgaatcgat
    22321 aatgacatta taaaattcct cccagtgcta gtacaatatc tttacgattc ttgtcaacta
    22381 accattgaat caatcgttta tctaataata tcggagtcat tcgtccatat tctttttccc
    22441 ctctgactaa tcctgtttca attagattcg tttgataagc ggcaataagc cgtttaatcg
    22501 tataatcttt ccattttgaa atatcttctg aacttctccg cttctctgta aaaaaagaac
    22561 ggtaatcaac tggtgttaat tgcaaatttc ctttacgtag attttcagca tatacctcag
    22621 tcataaattc attaaaaaga tcatcaatca taaggatgga aatcaaattc actaactttt
    22681 gattatctat atttaactga ttgaaaagtt gcaatccagc ttcatccagt tgactaactc
    22741 tccttttcac gttctgatag atttcttttt gacggttact gctcactgca aaaaaaatat
    22801 tttcatgttc atttttctct ttaatttcta tatctgtata tccttctagt cgtaattgaa
    22861 tatataactt gaactctgaa aaccaaaagc gtttagaaac tagaccagca gaatattttt
    22921 tattcaatag attgcctcct aatcatctat aattcaataa gtaaacaagt gttttttgct
    22981 catcgttagc attcattttt tagtatctac tatacaatac atatttatta ta
//
LOCUS       DASJTM010000046        22005 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJTM010000046 DASJTM010000000
VERSION     DASJTM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.046"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..663)
                     /locus_tag="U4B29_001638"
     CDS             complement(<1..663)
                     /locus_tag="U4B29_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2106588.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMD"
     gene            complement(812..1525)
                     /locus_tag="U4B29_001639"
     CDS             complement(812..1525)
                     /locus_tag="U4B29_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2106589.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            1700..3124
                     /locus_tag="U4B29_001640"
     CDS             1700..3124
                     /locus_tag="U4B29_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2106590.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            3423..4130
                     /locus_tag="U4B29_001641"
     CDS             3423..4130
                     /locus_tag="U4B29_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2106591.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4117..4440
                     /locus_tag="U4B29_001642"
     CDS             4117..4440
                     /locus_tag="U4B29_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2106592.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(4529..5566)
                     /locus_tag="U4B29_001643"
     CDS             complement(4529..5566)
                     /locus_tag="U4B29_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2106593.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5887..6870)
                     /locus_tag="U4B29_001644"
     CDS             complement(5887..6870)
                     /locus_tag="U4B29_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN2106594.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7285..8055)
                     /locus_tag="U4B29_001645"
     CDS             complement(7285..8055)
                     /locus_tag="U4B29_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106595.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8048..8983)
                     /locus_tag="U4B29_001646"
     CDS             complement(8048..8983)
                     /locus_tag="U4B29_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106596.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9299..10948)
                     /locus_tag="U4B29_001647"
     CDS             complement(9299..10948)
                     /locus_tag="U4B29_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2106597.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11065..13668)
                     /locus_tag="U4B29_001648"
     CDS             complement(11065..13668)
                     /locus_tag="U4B29_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2106598.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(13680..14729)
                     /locus_tag="U4B29_001649"
     CDS             complement(13680..14729)
                     /locus_tag="U4B29_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN2106599.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(14755..15069)
                     /locus_tag="U4B29_001650"
     CDS             complement(14755..15069)
                     /locus_tag="U4B29_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN2106600.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15085..15531)
                     /locus_tag="U4B29_001651"
     CDS             complement(15085..15531)
                     /locus_tag="U4B29_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2106601.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(15532..17454)
                     /locus_tag="U4B29_001652"
     CDS             complement(15532..17454)
                     /locus_tag="U4B29_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN2106602.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(17666..18562)
                     /locus_tag="U4B29_001653"
     CDS             complement(17666..18562)
                     /locus_tag="U4B29_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2106603.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(18555..19235)
                     /locus_tag="U4B29_001654"
     CDS             complement(18555..19235)
                     /locus_tag="U4B29_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2106604.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(19236..20018)
                     /locus_tag="U4B29_001655"
     CDS             complement(19236..20018)
                     /locus_tag="U4B29_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106605.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20011..20928)
                     /locus_tag="U4B29_001656"
     CDS             complement(20011..20928)
                     /locus_tag="U4B29_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2106606.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21092..21415)
                     /locus_tag="U4B29_001657"
     CDS             complement(21092..21415)
                     /locus_tag="U4B29_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106607.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6564 a         4515 c         3633 g         7293 t
ORIGIN      
        1 atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat
       61 aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag
      121 ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc
      181 aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt
      241 cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac
      301 aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc
      361 acacatatga taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt
      421 ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc
      481 catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat
      541 cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc
      601 caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc
      661 catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca aataatacat
      721 cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt agtataaaaa
      781 aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca gcttcaaaaa
      841 caaagtcttg atataaatgt ctcgataccg aatactcgaa aggtttacca gacgatagag
      901 caacaacttt gtctatctca atcataaatt cacctggatc tatttttaat atttttgcgt
      961 caaattcatt cgcagtatct cctttgatac gtatgtgcga agtctttatt tccagaccca
     1021 gttcatttgt gatataagca tatacagatt tctttaaatg ttttggttcc aagcctggta
     1081 tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg ttgacaatca ataaaacggc
     1141 ttcgtacaat attatataca tagtctttta cactgatact taatttatca gctacttttt
     1201 tcgatggaag ttcaattgag aaagtatgga tttctgaatc tacgtgttct tcaccaacca
     1261 cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt atcaattggt gttctgacga
     1321 cttcagtccc atacccactt ctacttcgca aaactccttc ggaaacaagt aagtctacgc
     1381 ctttctttac tgttaacttg cttgtatgat aaataactgc caattcatct cctgtaggaa
     1441 gtttctcacc aattttatac tctccattta caattttttt ctttatatct aagtaaattt
     1501 ccatatactt cgcaattttt ttcaatggtg accctccaat caaaaacata tcttcatcta
     1561 tactttaaca taaataaacc gataaaatcc acatcaaaat tatacacaca ataaaaaaga
     1621 tatatatatt taaacaaaac tatttacttt aagttgtaag cggtgttatc attaagtgga
     1681 aacaggaggt aaaaaaatta tgacaactat gaaaaagcta acttttccag atgatttttg
     1741 gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag gaaaagcaga
     1801 gactatctgg gatcgctggt ttaaagaaca accttaccgg ttttatcaac gaatcgattc
     1861 ctctattaca accgatcaca tccatcaata taaagaagat atagaactaa tgaaaaaaac
     1921 tgggcataac tcctttcgcg tttctatttc ttgggcacga atgtttccaa atggcggtac
     1981 cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa ttattttccg aaatgaataa
     2041 taacggaatc aaagtatttg ccaacttata tcattttgac atgcctgcta ctcttcaaga
     2101 taaaggagga tgggaatcac gtgaagtagt tgatgcttat gttcattacg ctgaaacttg
     2161 ttttaaagaa tttggtgact tagtttgtca ttggtttacc ttcaatgaac cacttgggcc
     2221 tattttaggt acttatcttg aagattttca ttatccaaac ctcattgact ttaaacgcgg
     2281 agcgcaagca gcattcttca ctatacttgc acacgcaaaa gcaatacaag tatttaaaga
     2341 actagctctt cctagtaaaa taggcgttat tctaaatttg agtcctactt ttcctcgtag
     2401 tcaaaatcct gctgatgtga aagcagcaga aatcgctgat ttattttata cgcggagctt
     2461 tttagatcct atggttaaag ggaatttttc taaaaagctt atcctgttat tacaagaata
     2521 taatcagctt cctgaagatt atacgaaaga agatcttgta tgtatctacg aaaataccgc
     2581 tcaaattctt ggactaaact attacgagcc acgtcgcgtc aaatctcgtt cttctatggt
     2641 ggatacatct agtccatttt taccagagtg gtttttcgaa ccacatatca tgcctggacg
     2701 acgaatgaac gaataccggg gatgggaaat ttacgaaaaa ggaatttatg atttgtgcat
     2761 ggatatcaag aaccattatg gaaacatcga agcatttatc tcggagaacg gaatgggcgt
     2821 tgcggatgaa gaacgtttca tggatgatac agggcaaata attgatgatt atcggattga
     2881 gtatatcaaa gatcatcttg cttacttatg gaaggcaata aatgaaggat gtaacattaa
     2941 ggggtatcat ctttggacat ttattgattg ctggtcatgg atcaacgcct ataaaaaccg
     3001 atatgggctg gtttcactcg accttactac tcaaaaaaga acaattaaaa aaagtggcga
     3061 attttacaag caattaagtg atacaaatgg atttagatac gatgtagact tgttagttga
     3121 ataatattct aaaattccac ttgaagtgaa cacattagaa aacaagaata atgagaaata
     3181 ccaataacaa atactttttc ttcatacttc atttatactt gtcagtataa gagactgtga
     3241 caaatgtagt gatcctcaga agttaggtct ggaaatctaa cttgagaaga tttttttaaa
     3301 ctacgtccag tcttttttat ttgtttaaaa ttgaagttga caagtcttca ttaaaaattt
     3361 agaatattca caagtttgac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt
     3421 tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct accgtttttg
     3481 gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccggg tttcatccgc
     3541 tggtcgtcac gttgatgtcc ctgctgatct atgctggttc tgcccaattt atcacggtca
     3601 gcatgcttgc tagccatagt ccactgctct ccatcgttct ctcgaccttt ctagtcaatt
     3661 cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc
     3721 aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat
     3781 tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt
     3841 atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc
     3901 cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc
     3961 aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt
     4021 tgattctggt atatgtcgga ctgattttta tcccaagtaa cttagtgatt ttagttgtaa
     4081 cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg
     4141 ctgactattt taggttgtac tttagcaact tggctttcta ggatcctacc atttgtctta
     4201 ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc
     4261 attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag
     4321 atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa
     4381 agcttattgg tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg cttattataa
     4441 ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca
     4501 aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt
     4561 ttcctaatat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca
     4621 tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag
     4681 caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga
     4741 acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct
     4801 ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt ttgatttgat
     4861 caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt
     4921 cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt
     4981 tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg
     5041 ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt gatcactaag atttttttcc
     5101 cgcggttggc attttcttct ggagtcagac caaatttttc aaataacaat tcataagcgg
     5161 tcaatgaggt caacggcaag gctgcggctt cttcatctga aagatttttt ggtgcaagag
     5221 cgacaatacg ttcgtcaacc agttggtatt cttgattgct tcctgctctt gtcgttgtac
     5281 ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcatcacct acagcaacaa
     5341 ctttcccgac tccatcaaac cctaagatac gtaaagcatt tcttatcccg ttatcttggc
     5401 gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg
     5461 aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca tcgatcgaca
     5521 acccttcata aaaaccgact accttcatta attccttttc tgacatacga attcctcctt
     5581 agcttcatca agttggagca tccatacagt tttcatttca atatatatag tatgctattt
     5641 gttccacttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
     5701 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttttaaacg gtgttcaatc
     5761 aactgaccct cggtattcgc tcaaaaaacg acaaaatatg aggagctatt tttgtttggc
     5821 tgttcttatt acttgatgca ggacagcttt ttcacgacct ctaattcacg gtgttcaagc
     5881 aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtgt ctaagatctc
     5941 tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat ttgctcctgg
     6001 aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac gcgggatgat
     6061 ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca gttctttttc
     6121 tgtatgttcc ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc cccggccaat
     6181 catgatgcca tctacgccat atttttcggc caattcttct cctgtctggc gatctgggat
     6241 atctccgttg atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag caatgatttt
     6301 agggatgact tcccaatgtg catctacttt actcatctct ttacgcgtac gcaaatggac
     6361 agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca tctcagacat
     6421 ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt tagccgcttc
     6481 aatcaattgt gccgctactt ctgggcgaag gatcaagcca ctgccttttc cacgttctgc
     6541 tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca tttctgccat
     6601 agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg caaccatcgg
     6661 ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg tcttttccat ctggatgaca
     6721 gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg catgctttac
     6781 gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa aaaaaggctt
     6841 tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc tatcaagcag
     6901 tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa aaagaagcaa
     6961 agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca aaaatcttat
     7021 cacaattcct tttccggaat aaatggtgtt caaaagcttg catctgcggt aataagtcca
     7081 accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa
     7141 cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
     7201 caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata ctcaggtcaa
     7261 aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat tgatcaaata
     7321 ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg atgttgctac
     7381 tggcacatcg attgttccaa ggaatccgta acggaaaccg gagatcatat aaacgatcgg
     7441 attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat agaaaacgcc
     7501 acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat cgtcataaga
     7561 ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg tcatcaataa
     7621 tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca aggaaatgat
     7681 cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa agccccaaat
     7741 gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct ggaatttttg
     7801 agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca tcataatcaa
     7861 accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc gaccaccgat
     7921 catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg
     7981 gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta atgcagtaaa
     8041 ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt ttcaaaaaca
     8101 gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct tgtcttgtca
     8161 gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg attgtctgtt
     8221 catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat ggcgctaaat
     8281 caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta gtattctcga
     8341 tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct tcttctaaat
     8401 aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga aactcccaca
     8461 tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc agcaaacgtg
     8521 gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg gaaagcatac
     8581 gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt tcgctgcgtt
     8641 tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg atttgctgga
     8701 ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt tgctttgctc
     8761 ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat gttttattga
     8821 ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt cctaatagcg
     8881 catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg accccacttc
     8941 cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc ctatctcctt
     9001 atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa cttggttcca
     9061 gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc aatcggacga
     9121 agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat tcatatgtgc
     9181 aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     9241 aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat cacttcgcct
     9301 atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt tttgcggttt
     9361 ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt cgggtccttg
     9421 aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt
     9481 caaagtggac attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg
     9541 cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa
     9601 tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag cagcgttctt
     9661 tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca aaataactgt
     9721 cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg
     9781 taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac
     9841 ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc
     9901 agttgaccgg attcactacc gcttctttta acaatggatg atagatatct ttcgctgtca
     9961 caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg taattcaata
    10021 ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct tcagttgaat
    10081 gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt
    10141 tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat gaaaaaccta
    10201 atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga agtggtgtcg
    10261 gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc atgctattca
    10321 gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat actaacccac
    10381 cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa cccaccagcg
    10441 gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg
    10501 ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga
    10561 ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct aactgctgat
    10621 cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct acactagaat
    10681 aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa gtaagatcat
    10741 cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc ttcaagcttt
    10801 cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga actgtttcct
    10861 ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct gcaataataa
    10921 ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt gttagcttta
    10981 gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa aataaaaggt
    11041 gtttttttct tgtatattac atatttatct ctcatttatc aaaattgaca aataatcata
    11101 gggaggaatt gttgttaatt cttgtttatt ctccagtgca ttgacaactg tcccaaggga
    11161 aaggatagcc tcaggtacta ttttttcttg ttctgttgga ttagctaagc gaagaacaaa
    11221 tccttgttca tgaaaataag aaggataaat cgatgataca attaagtctt caggtaattc
    11281 aatcaatgaa aatttacgag gaacatcaag ttcattttgt aatggccaaa tcttgttatc
    11341 taacctgtga ataaatacat tcagtttctg cttttgataa gaaatagatt gttctaaccg
    11401 tttttctgcc tgttttgcca ctttgaactc attaaatctt ccttcttcta cattaaatcc
    11461 aaaagtgact ttatactcac ctatctcttg agctagcggt gtttccatca tgacatgccc
    11521 ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa gcaagattag gttttccaag
    11581 ttcccctgtt gttgaaaata aggttatgat taattgattt tctatacgtt catactcttt
    11641 taaaccatct gcataaaacg taaaataatg atcattttct gccacagaga cactcttatc
    11701 gaatatttct aaattaacag gtttctctac catagtttct gaccaattcg gaggaatgac
    11761 atcaggggta ttttcaataa atccgttttg aatttgtgca attgattttt cagatttatc
    11821 taaaacatta attgctaagc gcatacgatg cgagtaaaca gtattatgaa ttttgagtgt
    11881 gccttcaacg atttgacttt ctcccagact gaacgatatt tcataagata tttcttccat
    11941 ttcgggtttt tcagataaac gatcttttaa atctttagga agttgagctt ttccgtaaac
    12001 aactaatgtt tcttgtaaag aatctttata tactttagct gtttctaatt ttagagatag
    12061 ttctgtatct ccttctaatg gtgaataatc atacgtatcg ccatcatttg ctgaatcgat
    12121 caaatgaacg aaatcaagat attgtctatc tccaactatc aaattcaatt gcccatcttt
    12181 aaaagaaagt ttatatttat tgttacttat ctcagttttg tctatctcga ttgctgtttc
    12241 taatttttca ttatttttct caaattcaac aacagtgtat cctagtgctg gcacatttac
    12301 atttattttt atgtcaagtt cataataagc tggttcatca aaatattcga aacctgtagc
    12361 aatcatccga taagcatttt tccgttctgg aaaataaact tctttttcaa tgatattata
    12421 gggactacct ttgaagtgta ttttctttga atcagctact acatgcacga ttttcgatcc
    12481 actataattc tcagtcgttg tgttgaaaac aaggacttca ttcgatttta gctttaatgc
    12541 atcagccatc cgttttacaa taaggttttc gattccgtca gctatctcat tagcttcttt
    12601 gattcgatgg aaaatatctt ccgcaacatt atctgaaaca cagccaccaa tactatcatg
    12661 agcctggttt tgcaaaatct ttttccataa ccgacgaagt aggcctttac tgatatggat
    12721 accatttctt tctgcaatca gcatgagcgg ctcaactcgt tttagaattt tttgttccaa
    12781 tttaaaattt tctattttca tttttgttct tactgagcca atagaacgat gaacacgaga
    12841 gtaaaccggc tgacgaagtt cacctttata ttcaggtaat tcttttttgt ttctgataac
    12901 ttctacataa gaaggataat ctgaaataat attgtggaat ttactgtatt tattcaactt
    12961 ctctacgact tgatcaaaat ttaaaatcat tgacttttga tctacacccg aaggtactaa
    13021 aatatctttg ttttttcctt gcatatgaag catttcaact gcttcatcca acttttcttt
    13081 tgcaaattca ggaatatctt taattgcgtc taaggtcatc attccagtca tataaccaaa
    13141 tggaaaattc atggcataaa caaatttatc tcccaatcct ttccagataa aataaggaga
    13201 tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg aaattatcga tttctacttg
    13261 atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg tcaggcaaat accctaccat
    13321 cattggatct ccatatttaa ggatcgtatc actgattcca atcattaagt ttcttaaaat
    13381 tgattcagca tccactaata aggcatctgt ttgtgcatac cacggtccca cgaatagttg
    13441 tcctgttttc atcagtcgtt ggataacctt aagcttttgc ggattgatct ccaaatactc
    13501 atcgacgatt gaagtttgtc catctaaaca aaagtttact ttagggttcc tttccaattc
    13561 atctagaact tcagtaaaga cctgatcaga caatacgatg gcatcttcat ttgtaaaata
    13621 ccattctctg tcccagtgcg tgtgttgaat tatatgtgca tttaccatat cagattctcc
    13681 tactttcctt cttctactga atttacttgt tgttcttttt tatacttgat ggattttagt
    13741 gcattgacca ttaacgctgt aaccactccc ccaataacaa ttgatactaa atataaaggt
    13801 atattcgtaa caaaaggaat tgccataaag gttgaaagca ttgtttcttg agtaacacca
    13861 aaaaatccat ttagaccaca tgctactgca gtgcccacca cgatgcttgg tattactcgt
    13921 aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa tccccacaag gcccataatc
    13981 atagctgtct ttgcattatc tcgttccgat tcatcataaa gttttttgtt taaaattgtt
    14041 gctaaaaaca ttccaaatgg cggaatagca attcctaata acatggctgt actaggtcca
    14101 aagcttccgc ttgaaaaaga agcaacccca aaagcatatg cgattttatt gataggtcct
    14161 cccatatcaa acgccatcat tcctcctaaa atagccgaca tcaaagcagc atttgctcct
    14221 gacatgcctt ctagccaatt agtcagtcct gttgtcattg cttgaatagg cgtagctagt
    14281 acataccata atatgattcc aacaactaat gtagtaacaa aaggaatcag cataattgat
    14341 tttagactaa taacttgcat cggtactttt acttttttca atgcattagc taaatagcca
    14401 caagctaatc cagcaaccat tcctccaata aatcccatac caagattttg acacatcatc
    14461 ccagtgataa aagctggtgc tagaccggga cggtcagcaa ttgaatatgc gatataggct
    14521 gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa tcattcgcag gactccccag
    14581 aatgtatccg tctggtccca aacacctgtg ccaccaaaaa tgactgcaat agaagttagt
    14641 aaccctgctg ctacgacaag cgggatcata taggaaaccc cactttggaa atgatttaga
    14701 atttctcttc cttgtttctt gaattccatt agtaactccc ccacgatata ttttttattt
    14761 gttaattaat gcaattgctt catcaatcgt ctcttcacca tcttttacta cctctgaggt
    14821 ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt tctgaaacat ttgtatctac
    14881 tgcaaatatg acaacatctg cctcggaaac atccgaaatt gaaagtttgt tttctatccc
    14941 catagccccc tgtgtttcta ctttaatatc taaccctttc ttcttcgcaa acttttctaa
    15001 attagctttt gccatataag tatgggcaat ccctgcgata catgctgtta ctgctactat
    15061 ttttgtcata tgaaattcct ccttttaaaa tgatagagct tttaccaatt cttttttatt
    15121 tctcgaatct tttattcttt taacaaactc gtcatccatc aatcttccag ataattctgc
    15181 taaaatacgt aaatgattag tacctttatc agtgtctttt attgctaaca aaaagacgag
    15241 atcaactggt tgatcatcta atgcgcccca ttcaacagac cgctttaact tggctacagc
    15301 cactgttgat tcaattactg ctaggctttt gccatgagga atggcgatat ttttcccaat
    15361 tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt tgaaattgtt tgtggtcaga
    15421 cacaaaccca ttagtctcta acaatttcga taatttttcg actacacttt ttttatcaga
    15481 tgcatttaaa tctatcacta tattatttat attgattttt tcactgatca tttataagcc
    15541 ttctttcaat atataaacta atttttcaaa cgacgaactc gtttttaaat ttgctaataa
    15601 ctcatgattc gaagcgattt cattgaatgt ttcataaatt ttattcaatt caggttttac
    15661 tttttcatta agtgcaaaaa agaaaacaga atgaacaagt gtcccatccc aatcaattcc
    15721 ttctggatta acgtagacac agattactgg cttttttata aaatctactt cgatatgcgg
    15781 catagcaaaa tcattcatcg ctgttgaaga tagtttttct cttttgattg ctccctctag
    15841 tatcccttct ttcgcgtaat tttcttttac caattttttg gtaattatat ttaaaacgtt
    15901 cacttgatta tcctttattt ggtgggtaaa aaagcaaaat cgtttatcta ataaatttag
    15961 aaaagcatct tctgtttgtt catttaactg tagatactgt tttacttttc tgacatctgc
    16021 ccctaacatc aaaggactaa caacaataac aggaaaattt gtattttcaa tcggaatcgt
    16081 gcttataaca agttcatcag tgacttttct atcttgtagt tcattgatgc ttagtacatc
    16141 ttctatctca accatatctg caaaaccgtt atttattctt tgctctaata attttgatgt
    16201 tccatatccg ctactacaaa ctaatatcac tttcgttttg tcaggtttgt aacgatccaa
    16261 aagtgattga atatggagtg ttacgtaagc gatttcatct tcattaaaat gtatacagaa
    16321 tctctcttcg atttcctcta ataaatctac acttatcata aatgaccttt taaaagaata
    16381 tttgattttg tccgtataag ggttatagat atttactccc aatttgagtc ttttaactgc
    16441 tgcattgaga tgtaatacta gattattgat caattcttca tcaggataaa attcatttaa
    16501 tttaattctt actaactctt gtagctcaac acccatatct aaataggaaa gcttcgtttg
    16561 atctaagtcg tgattcatag catttttttc aatcgaagta atgtgaatgt caacataagc
    16621 ttgttcatct attggcacaa taattgaaaa tgctttttca atcattttga ttaaaataag
    16681 tgtattcttt gagatttctt cttcagaatt attactacgt gataagaaat tgcttgtttc
    16741 aatcctttct atcgcaatag caaggtggat agctaaagtt tgaaattcat aatctgtaaa
    16801 tttcaattgt gtcttattca tgtatcgatt aagcaattcg gctacttttt tcaatatgtc
    16861 cttatttaag aaattattcg cttcaccaat gaaattgata ttcaagtcta agagatctgt
    16921 tttactgtta gatgctgtaa agccacccca ataatagttc acaacttctg acatcaacct
    16981 tcttttactt ttttcatcag ctttgatgcg cacgccattt cgatctgaaa ctaaggacat
    17041 cccatgcttg tttagtcttt ttctaacatc tttcattgtg ctttctaccg tcgacctgct
    17101 cacgtataat tcgtcagcaa gattttgaat ctttagagaa tcattagtat tcaataattt
    17161 agcaaacaca taagttagac ggtcctccgg agagtctgcg atattgattt tatgactgga
    17221 taataaattt cgaagtgttt ctttttttcc aataagcgaa acgccttttt taggttgaac
    17281 caacatttgt acatctggca ttttttggat tatttctttc aaaattaaaa tatctttgct
    17341 aattgttttc gtagatacat ataattgttc tgataattct ttgtaagttg tgtgctcatt
    17401 ttcaatcaaa aagtatagta ttttcatttg ccgttcgcta aattcgatgc ccattatctt
    17461 cacctcctga atacagctta agtgtacaat tattatctct attttcatac agaagaaagt
    17521 tcttgattaa tgtagaagtt tctaatattc ttgattattt caaatcaata aaaaagcctg
    17581 caatatttat atcacaggct tttttctcta ttaaatatat ataagataat acatttttct
    17641 actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag atggagaaga
    17701 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
    17761 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
    17821 gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg gccactgtta
    17881 atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
    17941 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
    18001 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
    18061 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
    18121 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
    18181 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
    18241 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
    18301 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
    18361 cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
    18421 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
    18481 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
    18541 gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
    18601 gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac tgttttctca
    18661 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
    18721 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
    18781 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
    18841 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
    18901 aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
    18961 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
    19021 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
    19081 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
    19141 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac agcttcatcg
    19201 tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga tttttggaaa
    19261 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
    19321 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
    19381 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aatgaaggcc
    19441 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
    19501 gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac aggaaatact
    19561 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
    19621 gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt gatcaaaagc
    19681 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
    19741 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
    19801 agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc gttgatgact
    19861 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
    19921 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
    19981 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
    20041 taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag gaacttttgc
    20101 ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
    20161 acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
    20221 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
    20281 cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg cgatctgtga
    20341 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
    20401 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
    20461 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
    20521 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
    20581 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
    20641 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
    20701 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
    20761 acccataata gttctcataa aggtagactt acccgctcca ttttccccaa tcaatccata
    20821 gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag caccgtattt
    20881 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
    20941 ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
    21001 agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata cgtgtctttc
    21061 ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc ttttcgacca
    21121 aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga tcaagcttga
    21181 gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc agaaaaatta
    21241 gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca aacagtgcta
    21301 atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataagt cgtccttgta
    21361 tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa aacattaatc
    21421 cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat tagctaccaa
    21481 ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac ggtgttctcc
    21541 tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa
    21601 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg gataaataat
    21661 attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga tattatagac
    21721 caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc aaattgtgga
    21781 aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat
    21841 tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac tgtgaagctt
    21901 tacgggcttt cttaagacct ggttttttct tcgtatcatt gaataagttt gaaaaacctt
    21961 gatttataaa ggttttcccg taaatatatt ttattatatt tgatt
//
LOCUS       DASJTM010000047        21907 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJTM010000047 DASJTM010000000
VERSION     DASJTM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.047"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..1151
                     /locus_tag="U4B29_001658"
     CDS             147..1151
                     /locus_tag="U4B29_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106608.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1152..2096
                     /locus_tag="U4B29_001659"
     CDS             1152..2096
                     /locus_tag="U4B29_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106609.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2096..3058
                     /locus_tag="U4B29_001660"
     CDS             2096..3058
                     /locus_tag="U4B29_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106610.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3085..3999
                     /locus_tag="U4B29_001661"
     CDS             3085..3999
                     /locus_tag="U4B29_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106611.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4025..5806
                     /locus_tag="U4B29_001662"
     CDS             4025..5806
                     /locus_tag="U4B29_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2106612.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6154..6840
                     /locus_tag="U4B29_001663"
     CDS             6154..6840
                     /locus_tag="U4B29_001663"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN2106613.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6860..10441
                     /gene="smc"
                     /locus_tag="U4B29_001664"
     CDS             6860..10441
                     /gene="smc"
                     /locus_tag="U4B29_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN2106614.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10450..11259
                     /locus_tag="U4B29_001665"
     CDS             10450..11259
                     /locus_tag="U4B29_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2106615.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11270..12268
                     /gene="ftsY"
                     /locus_tag="U4B29_001666"
     CDS             11270..12268
                     /gene="ftsY"
                     /locus_tag="U4B29_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN2106616.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12455..14722
                     /gene="gshAB"
                     /locus_tag="U4B29_001667"
     CDS             12455..14722
                     /gene="gshAB"
                     /locus_tag="U4B29_001667"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN2106617.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14811..15263)
                     /locus_tag="U4B29_001668"
     CDS             complement(14811..15263)
                     /locus_tag="U4B29_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN2106618.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15413..16363
                     /locus_tag="U4B29_001669"
     CDS             15413..16363
                     /locus_tag="U4B29_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2106619.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16356..17315
                     /locus_tag="U4B29_001670"
     CDS             16356..17315
                     /locus_tag="U4B29_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2106620.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17312..18067
                     /locus_tag="U4B29_001671"
     CDS             17312..18067
                     /locus_tag="U4B29_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2106621.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18090..19043
                     /locus_tag="U4B29_001672"
     CDS             18090..19043
                     /locus_tag="U4B29_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2106622.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19687..19983)
                     /locus_tag="U4B29_001673"
     CDS             complement(19687..19983)
                     /locus_tag="U4B29_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN2106623.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20338..20694
                     /locus_tag="U4B29_001674"
     CDS             20338..20694
                     /locus_tag="U4B29_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2106624.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20704..21603
                     /locus_tag="U4B29_001675"
     CDS             20704..21603
                     /locus_tag="U4B29_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN2106625.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7247 a         3863 c         4663 g         6134 t
ORIGIN      
        1 tgcaaagtaa agcggttttc tatataatta ttaataattc attacacaaa gataagaata
       61 acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca
      121 tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc
      181 tacatacagg attccgactc aaagatgact attacgatgc agtagatgac gtttcattta
      241 ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct
      301 tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca ggtgagattt
      361 tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa
      421 atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg
      481 aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag
      541 ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc
      601 aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg
      661 cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc
      721 aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct
      781 tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg
      841 gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt
      901 atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg
      961 tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac
     1021 cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg
     1081 caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag
     1141 gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc
     1201 gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta
     1261 ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta
     1321 cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa
     1381 aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga
     1441 tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg
     1501 aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac
     1561 tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag
     1621 gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg
     1681 tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca
     1741 tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg
     1801 tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg
     1861 gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag
     1921 ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag
     1981 aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt
     2041 tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg
     2101 gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt taagtgttct
     2161 gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga tcaatccaaa
     2221 tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg acccatggta
     2281 tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc aaagtttctt
     2341 atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt tatatctatc
     2401 gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc ttgctggccg
     2461 gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta gttttgctgt
     2521 tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt taggctggtt
     2581 cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc aatggataga
     2641 ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga ctgccgtcac
     2701 aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc
     2761 ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc gaaatgcagc
     2821 gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg gttctgtagt
     2881 tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt ccatcggcca
     2941 acgggactat accgtgatca catccttagt attgatttta ggaatcgcga cgctagtggg
     3001 gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga ttcaataaaa
     3061 ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt
     3121 attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa aattgctatt
     3181 ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc gatgctgacc
     3241 gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc aggaacagtt
     3301 tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga tgtattagga
     3361 caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac gatccttact
     3421 tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat cattgacaac
     3481 atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat cattatcgtc
     3541 ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat gagtgcattt
     3601 tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc aagaagagat
     3661 tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc
     3721 atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc ggcaaacatc
     3781 ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt acctagttta
     3841 ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca atggatctgg
     3901 ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt tggacaagca
     3961 ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga agggggaaat
     4021 aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc
     4081 atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg gcaataacaa
     4141 agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg aagacggcga
     4201 attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt gggagtttta
     4261 ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat ttgcaaacga
     4321 tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg aagatacgaa
     4381 aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag atgtaacggc
     4441 ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg gtatccgtta
     4501 tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa gtgccgacac
     4561 aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata aagaagtaaa
     4621 cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa aacatacgtt
     4681 agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa atccagtgac
     4741 ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa
     4801 cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat
     4861 ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc aaatgccaac
     4921 agatacttac gatacctata aagaaattgc tggctataca aacttaggcc gtgaacaaac
     4981 ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa agagtgtgga
     5041 atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg gctatgcatt
     5101 agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg cgacttcatt
     5161 gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca cacaagatct
     5221 agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg acgatggttt
     5281 aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt ccggtgggga
     5341 aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg gtttggatgt
     5401 ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat tagaaaacga
     5461 tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc caaatccaag
     5521 tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac
     5581 aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc aaaaagaagc
     5641 atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa cgttgttccg
     5701 taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt atgatgtacc
     5761 aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac taaaaatgac
     5821 cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt tctctaatat
     5881 tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag tccagtttct
     5941 cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc
     6001 atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat tattaaagct
     6061 ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata ataaggaagt
     6121 atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac aaaagaacta
     6181 aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc ttttacccat
     6241 tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg acttgaattt
     6301 ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt atatcctcat
     6361 ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg
     6421 aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg agaagagaat
     6481 tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt tttaggcgcg
     6541 ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt gatttttcct
     6601 aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact acaagaagtc
     6661 ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga aggtccagct
     6721 catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg cactggacaa
     6781 gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc agcactgtaa
     6841 aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt ttaaatcatt
     6901 tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg
     6961 cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac aatctgctaa
     7021 aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata cccgtaagca
     7081 gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc tgccattgga
     7141 gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg agtttttcat
     7201 caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt ctggattagg
     7261 aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca gtagtaaacc
     7321 ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca agcagcggaa
     7381 aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg tccaagacat
     7441 catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg cagcaaagga
     7501 gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg ttgctgaaat
     7561 caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat tcaatctaga
     7621 attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga agcagcgtaa
     7681 agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat tagatctttc
     7741 ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga caaagcatac
     7801 acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa aggttaaaca
     7861 ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa cagagatcca
     7921 aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac
     7981 gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag aacaagcagc
     8041 tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag caaaaagcaa
     8101 gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga tcttacaaaa
     8161 agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa caaaagagaa
     8221 gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa aagaacagcc
     8281 taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga aaagccttca
     8341 agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct tgcagcataa
     8401 acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc ctgcagattt
     8461 cacgttagct atcgaagcag ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga
     8521 aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa gagcaacatt
     8581 ccttccactg acgacaatca aaccaagaca attaccggct catatattga cacaagcagc
     8641 agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg atcaaatcca
     8701 aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa ctagtgccaa
     8761 tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag gtgatgtgat
     8821 gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag gcagtttgtt
     8881 tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag ataaacaact
     8941 ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt tagccgaaga
     9001 acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag aagcgacaaa
     9061 tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc aaatctcaac
     9121 ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa cgaacgaatt
     9181 gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc
     9241 gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa gtcaaaaagc
     9301 acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc agatccagct
     9361 ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga tagaaaagca
     9421 gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa gtttggaaaa
     9481 acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg caaaagccaa
     9541 agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct tggcagagcg
     9601 taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg
     9661 agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc agctgacatt
     9721 tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta gaacaaaggt
     9781 ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt caatcgaaca
     9841 atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg atctgttagc
     9901 agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc ggacaagatt
     9961 caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa atatgtttgg
    10021 cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa caggtattga
    10081 gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga
    10141 acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt
    10201 ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg
    10261 ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc gcaaaggaac
    10321 gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg tatcaaaaat
    10381 cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta
    10441 ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt aaatgaacaa
    10501 aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca aggggtcaag
    10561 atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca agaattagga
    10621 ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca aaaaaatgat
    10681 actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg cttatacaaa
    10741 ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt tttacagctg
    10801 cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct gcaattccaa
    10861 ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt aatcgcttat
    10921 gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca tgaaatgtat
    10981 gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt tacaaaagct
    11041 tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat catggcgatc
    11101 ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt agcgatggag
    11161 aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa tgaagaaaat
    11221 ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa tgggattttt
    11281 tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg aagtacagga
    11341 gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt
    11401 gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag aaacacttat
    11461 cggtgcagat gtaggttttg aaacagcgat acgaatcacc gaagcattac gtcaagaagt
    11521 gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg agaaacttgt
    11581 ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg
    11641 actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa
    11701 acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag cagatacctt
    11761 ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt
    11821 tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg aaagagcaaa
    11881 gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa ataaagtgaa
    11941 cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac cagaagcacc
    12001 ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa
    12061 acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag atggtacagc
    12121 aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga aattggtcgg
    12181 tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg tcgttggatt
    12241 gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc tgtgacaaaa
    12301 gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga tgccttcatt
    12361 tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag actttttgat
    12421 tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc aattattatt
    12481 gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac gggaaggaca
    12541 gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct ttggcgatcg
    12601 atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga tgatcacccc
    12661 tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg atgtgactgc
    12721 tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg ccttaccgga
    12781 aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg
    12841 ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc atttcaattt
    12901 tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat ttcaagattt
    12961 ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc gctatcgctg
    13021 gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact ttttagatga
    13081 atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct atacgaatca
    13141 tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata tcgagcgaat
    13201 gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc gtttccgagg
    13261 cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat tgcggaatat
    13321 cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt tcttacaatt
    13381 attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat gggtagcaga
    13441 aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa cagaatttca
    13501 tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat tggattggtt
    13561 ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg aacaaacgtt
    13621 agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg ctgtggcatt
    13681 gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg gttttcgcga
    13741 aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa
    13801 agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg tagaatatgt
    13861 gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca tggaaaacaa
    13921 aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag gcgcagaatt
    13981 ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg catttgtgat
    14041 caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag gagctagtct
    14101 ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg ttttagtaga
    14161 agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag tccaagccat
    14221 catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga aagaacttgt
    14281 tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat
    14341 ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga tcgaatcagt
    14401 tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta caggtggtga
    14461 ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg tttctgcggt
    14521 agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata aagaaatcga
    14581 tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc cagccatgca
    14641 tatgcatgtt tatccttttg caggaaaagg cagaagattg acgatgaatg tattgaaatt
    14701 gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta tttgatgtca
    14761 atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa
    14821 cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga agagttttct
    14881 tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt tgctgttaag
    14941 agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt gaaacggcta
    15001 ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag agccccattc
    15061 aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc tttgtttttt
    15121 ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa tgtccctaaa
    15181 tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt atccaaatgg
    15241 ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt ctttcttacg
    15301 attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct tttgttcaac
    15361 gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta
    15421 cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca tcggagtatc
    15481 gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa tcttgctagt
    15541 gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta gtatttgtgg
    15601 gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg cgggcacgat
    15661 ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg catccttgat
    15721 tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt ttttatccct
    15781 tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag tcatgttagg
    15841 gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg tacaaaatat
    15901 gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg agttattgta
    15961 tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta cgatcattgg
    16021 tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc agtttttagg
    16081 cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa gcattccttt
    16141 tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag tgcacaaaac
    16201 aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc ttgtcgctcg
    16261 cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca tcggtggggc
    16321 tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag ccatctattt
    16381 ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg atccatacat
    16441 atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca tttatccttg
    16501 tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat aaccagtttt
    16561 tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg gtccttttct
    16621 tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc ttattgaacg
    16681 ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa aaaatgagag
    16741 cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt ggtagtgtca
    16801 gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa ggcagattat
    16861 ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg atcattatcg
    16921 gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat ctcggaaatg
    16981 aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt ttatgttgtg
    17041 tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt ttagggttta
    17101 ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag ctatttatcg
    17161 gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta gagcagattt
    17221 tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca
    17281 caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaagg tcaataaact
    17341 gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa agaagttgac
    17401 tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt
    17461 gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg cttggaaatc
    17521 caatgaatta gccaaaaaat tatcgatcct acgacaatcg aatgaaataa atgtccaaat
    17581 caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa aattgacgac
    17641 aaaagacgag gaaatcgttc aggaatcact ggaacacatt gggttagaag aaatagcgga
    17701 tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg caatggtgct
    17761 agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag atatgaattt
    17821 tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt
    17881 tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg ttgcaatgaa
    17941 agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag atgtattgga
    18001 tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat tctgtcttta
    18061 tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt atcttgattc
    18121 tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag gcagacacaa
    18181 aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat ggagaccaaa
    18241 tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct ttagatacga
    18301 tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc cttcctgagt
    18361 atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca gacttagaaa
    18421 aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga gattttcaaa
    18481 aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa aatccatggg
    18541 aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa gaagaagcta
    18601 aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta
    18661 ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc tacggatctg
    18721 gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat gatcaaatcg
    18781 aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa aatccggata
    18841 tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa gatgatattt
    18901 ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc atctcccttg
    18961 aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg atgatcgaag
    19021 atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca tactatgtaa
    19081 caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa
    19141 ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg agaaatcttt
    19201 tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg gagtggcaaa
    19261 aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga atcaaaggta
    19321 caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc gatctcttct
    19381 ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag aaaatatgag
    19441 aagctatttt ctgtccagcg ttcttattac tcaaaacaaa tcgcagttgt cacaatctct
    19501 tctaaacgat gttccatctg ctgcttctcg gcaataagcc cactagacta gaaaatatga
    19561 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    19621 ttctaaacaa tgttcaatca gctgaccttc ggtattagct caaagcactg atttcacaac
    19681 ctctatttag gcatgtgtct cagcaagcgg ttgatagttg atgatctgca tttttcgata
    19741 aaaatcggcg ggaagcttac tccaccgttt tagtaattga tagaaataac tctcttctgc
    19801 tttgatttta tactcgctta atatcaatcc aaaactattc gttttttttg aagctaattt
    19861 tttataaata tccaatgctt tggttttatc ttcattgaca aagagtgttt ttacttcaat
    19921 ataagaaccc tcgatatttt gtgtttcttc gataaaatag atgtttttta tatattcctt
    19981 catagtgatt cctccaaaga agtcttttat ttgataagtt tactctaccg aagagttgtt
    20041 aataatccgt gaacaaaaag taattctttt tccaatagtt gttaaaggtt ttttattttt
    20101 tatagtattt tttgttattt aagaaaggaa taaggttaaa caaataacag gttgcttttt
    20161 gggtcttttt ccgttcaaga aactgaacga agaaaaggga aagttgtctc ttttgtgata
    20221 agagaaatca taataaacga atgacttact ttccaataaa ttgagaacag tcagaaccag
    20281 acaaattagt tgcatctgat gaataaagtg attattctat gacatgaggt gatacagatg
    20341 aaattagaag tgacaaaaga agcacaagaa gtattgaaga ataaattaga agcagacgat
    20401 caacttttat tagatatcga aaacggtgac ggtccatttg cagaaagcca agtcagttgc
    20461 caattggata cggcatttcg attgatcatt gtcaaaaaag atacacaaac agatctaagt
    20521 ctttattctg tagtggctga tacaccagtt ggaccaatca aaatcaaaga cagtgcaata
    20581 ctttacatgg atgatccatc cacacttgct ttagaaccaa cttataatag tttacaatta
    20641 aaaggaccaa gcggcttgag aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat
    20701 aaaatgggaa aattggcgat catcagcgac ctacatgtgg acatcaataa attcggcgaa
    20761 tcagagctga tgcagctttg gcaagttttg caaaagcaca agatcactcg gcttcatcta
    20821 gccggagata cggctaataa agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat
    20881 aaaggattat ctacgacatt tcatttcggc aatcacgagt tggcagatgt gcccaatgaa
    20941 cagatgatgg accattttcc agacccacat tttttaaatg aacggtatat tgctttaaat
    21001 gagcagaccg ttcttttagg gatgaatggc tggtacgact atcagttttc cgaattgaaa
    21061 gattacgagg caatcgtacg aatgaagcgt ttgtattggt atgatcggtt catccagcgt
    21121 gggaaaacag atctcgaggt caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac
    21181 cagctggaag caaaaaaact tgaggtgatt ttatccactc atttcgttcc taaacgggca
    21241 tttatcatgt atcaatctgc tccttatgag cgttggaaca aattaaatgc atttttagga
    21301 tcagaatcat tcggagaatt acttgaccgt tattcaaata tcaaacaagt agtttttggc
    21361 catacgcatc gtcgctttga agataaatcc atcggtcaca cgatttacag ctgcagacct
    21421 tttggttatt attatgaatg gcagctgacc cgagattttg tcttgaaaga acagttgcta
    21481 gacagctatg atcctatgaa attacgtgtc ctcttgcgtg cccatcaccc agcttttcag
    21541 gcctatcaaa cacaacattt gcaagaggaa tttgaacaag cgatgacaat cgttccgtat
    21601 tgagacagca ttagtaatgt gtaaaagaaa ataccttgta aaacataagg ataagtaaga
    21661 gcagtagatc catttatcgt ggatatgctg ctttttttgc ggaaaataat cagcgaatgt
    21721 ttatttgatc ggatgatttg taactcgcag agtactagat gcaagcgtgt attctttttt
    21781 tgaacgataa tcacaaaaaa aaatataata tacatttttt atttaattat aattaatatt
    21841 gattgaagtc taggcagtca agtcgagagt gtaaaatatt ttgtgtaaat gaaaaaatcc
    21901 atacaaa
//
LOCUS       DASJTM010000048        20360 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJTM010000048 DASJTM010000000
VERSION     DASJTM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.048"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            291..1430
                     /locus_tag="U4B29_001676"
     CDS             291..1430
                     /locus_tag="U4B29_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN2106626.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="U4B29_001677"
     CDS             complement(1497..2072)
                     /locus_tag="U4B29_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2106627.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="U4B29_001678"
     CDS             complement(2077..3051)
                     /locus_tag="U4B29_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN2106628.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="U4B29_001679"
     CDS             3334..4302
                     /locus_tag="U4B29_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2106629.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="U4B29_001680"
     CDS             4415..4693
                     /locus_tag="U4B29_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2106630.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="U4B29_001681"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="U4B29_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN2106631.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="U4B29_001682"
     CDS             5150..5905
                     /locus_tag="U4B29_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2106632.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="U4B29_001683"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="U4B29_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN2106633.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="U4B29_001684"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="U4B29_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN2106634.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="U4B29_001685"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="U4B29_001685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN2106635.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="U4B29_001686"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="U4B29_001686"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN2106636.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="U4B29_001687"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="U4B29_001687"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN2106637.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="U4B29_001688"
     CDS             10642..11184
                     /locus_tag="U4B29_001688"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN2106638.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="U4B29_001689"
     CDS             11207..12763
                     /locus_tag="U4B29_001689"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN2106639.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="U4B29_001690"
     CDS             12777..13679
                     /locus_tag="U4B29_001690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN2106640.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="U4B29_001691"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="U4B29_001691"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN2106641.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="U4B29_001692"
     CDS             15122..15544
                     /locus_tag="U4B29_001692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN2106642.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="U4B29_001693"
     CDS             16013..16243
                     /locus_tag="U4B29_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN2106643.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="U4B29_001694"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="U4B29_001694"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2106644.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="U4B29_001695"
     CDS             17654..17830
                     /locus_tag="U4B29_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2106645.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="U4B29_001696"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="U4B29_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN2106646.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="U4B29_001697"
     CDS             18325..18708
                     /locus_tag="U4B29_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106647.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="U4B29_001698"
     CDS             18877..19761
                     /locus_tag="U4B29_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2106648.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="U4B29_001699"
     CDS             19875..20111
                     /locus_tag="U4B29_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN2106649.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="U4B29_001700"
     tRNA            20199..20279
                     /locus_tag="U4B29_001700"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="U4B29_001701"
     tRNA            20286..20357
                     /locus_tag="U4B29_001701"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DASJTM010000049        19691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJTM010000049 DASJTM010000000
VERSION     DASJTM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.049"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..207)
                     /locus_tag="U4B29_001702"
     CDS             complement(<1..207)
                     /locus_tag="U4B29_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106650.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFD"
     gene            complement(213..443)
                     /locus_tag="U4B29_001703"
     CDS             complement(213..443)
                     /locus_tag="U4B29_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106651.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1060..1722
                     /locus_tag="U4B29_001704"
     CDS             1060..1722
                     /locus_tag="U4B29_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106652.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1801..5028
                     /gene="fss3"
                     /locus_tag="U4B29_001705"
     CDS             1801..5028
                     /gene="fss3"
                     /locus_tag="U4B29_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2106653.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5142..5456
                     /locus_tag="U4B29_001706"
     CDS             5142..5456
                     /locus_tag="U4B29_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2106654.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5469..5843
                     /locus_tag="U4B29_001707"
     CDS             5469..5843
                     /locus_tag="U4B29_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2106655.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5853..6197
                     /locus_tag="U4B29_001708"
     CDS             5853..6197
                     /locus_tag="U4B29_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2106656.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6268..7620
                     /locus_tag="U4B29_001709"
     CDS             6268..7620
                     /locus_tag="U4B29_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2106657.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7680..8822
                     /locus_tag="U4B29_001710"
     CDS             7680..8822
                     /locus_tag="U4B29_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN2106658.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8847..9101
                     /locus_tag="U4B29_001711"
     CDS             8847..9101
                     /locus_tag="U4B29_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106659.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9357..10541
                     /locus_tag="U4B29_001712"
     CDS             9357..10541
                     /locus_tag="U4B29_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2106660.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10538..10675
                     /locus_tag="U4B29_001713"
     CDS             10538..10675
                     /locus_tag="U4B29_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2106661.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10680..10901
                     /locus_tag="U4B29_001714"
     CDS             10680..10901
                     /locus_tag="U4B29_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106662.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10914..11414
                     /locus_tag="U4B29_001715"
     CDS             10914..11414
                     /locus_tag="U4B29_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2106663.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11511..13358
                     /locus_tag="U4B29_001716"
     CDS             11511..13358
                     /locus_tag="U4B29_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN2106664.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13361..14257
                     /locus_tag="U4B29_001717"
     CDS             13361..14257
                     /locus_tag="U4B29_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2106665.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14307..14696
                     /locus_tag="U4B29_001718"
     CDS             14307..14696
                     /locus_tag="U4B29_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2106666.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14683..16185
                     /locus_tag="U4B29_001719"
                     /pseudo
     CDS             14683..16185
                     /locus_tag="U4B29_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16930..18756
                     /gene="ltrA"
                     /locus_tag="U4B29_001720"
     CDS             16930..18756
                     /gene="ltrA"
                     /locus_tag="U4B29_001720"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2106667.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18890..>19691
                     /locus_tag="U4B29_001721"
     CDS             18890..>19691
                     /locus_tag="U4B29_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2106668.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6654 a         3219 c         4307 g         5511 t
ORIGIN      
        1 atcaaacaaa aagtatacat ctttcttcat tctagttttt gcagcaggga ttttaaagta
       61 gttcttatta gaatagtaga gattgattaa taagctatct tcgattgcta gaaagaaaac
      121 ttctgaatcc cacaccttat aaaaatcttt gataaatcta ttcgaaggat caaatttaaa
      181 ccataattgt gtctcattaa aaagcatagc cattactccg atctattttt aaacttagtt
      241 tctacctcta atatatatcg agtttttatt ttgccttcag ataatactgt ttcttttttc
      301 gcaattacag gttggttatt ttcggaaaaa gctaatatag ctaagattga aacatccatc
      361 tggaatttat cttttttgct gctttgctca taaaagtctg catattcatc actgatattt
      421 tttctaataa agtcttccat cataaaaatc acctcaaaac gattatacga acttacgttc
      481 tcgttgtaaa gcgatatttg aggggcaaaa agggggcaaa aatacgttta cctttgatat
      541 atctcagatt gaacatagtg tttattatat gtcggtattg ttgatttata gctgttttga
      601 gggtattaga tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct
      661 gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg
      721 tgaaaagtcc ttgtatatca agggcttttt cttttttctc tatcacagtt ttaagccatt
      781 tttggctgtt ttggaccatt tttcaatcat attcccttaa aaattgtcct agattgtccg
      841 acattgacta tcagtgtcgt caaaataggg caaaatttag tagtcagctt gacgacaaat
      901 ataatacgaa aaataccaaa acatttaccg aatttagcaa cttttgaacc caagtttctc
      961 aaagggtcag ctattagcaa aaaagacaaa acatgtactt ttctactaaa aagttgatgt
     1021 gataggcttc tatctaaata actattagga gtttttatcg tgaagcaaaa tgatggacgt
     1081 atcatttgga aaaatcaaag tgagttaaaa ctgattttaa caattaatga attcatcgaa
     1141 aaacacggaa ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa tccaaattct
     1201 gcaccaagta tgtggttcat taacaaaaaa tatggatcat gggagaattt attgattagt
     1261 ataggacgag aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca agaattattg
     1321 gaaatagtag aagcctttat taagtgcgag aagataacct ctcaacgaat gtacgaacaa
     1381 aaatcagtag ggaagaatat tccttcactt agtaccatta aaaagatgtt aggagatata
     1441 cgtccacttt ttaaagaaaa aaatgatgga tctcgattta ctgatttcga actgctgtta
     1501 gaactaaaaa atgaaataat ccgattaaaa ctacaggatg atttatcaat gacaaagttt
     1561 cgaaaactag ttcaatcccc aaaactacca tcagttgata caattatgaa aagaacgaac
     1621 aaaaattggg aggaattgat ggcagagatt ggatttgatt atcgaagaat caaaatttat
     1681 aagcagagaa acaacctatc caagacaaag aaaactaaat agtatcactc taaatcagac
     1741 gttcaaaatt aaaatttgga cgtctttttt attacccaaa aacgaaagga gatttatttt
     1801 atgaaaaagc caagatttaa gaattggcgt ttgctcgcaa cactgagtct attatgccaa
     1861 acaattggtg gatcgcttgg cccaacgatt gcctttgctg atgaaattac tcatccacaa
     1921 acggtaactg tggaattgga tttggctcac cagtatgctg tcgaaggtac atttagcgat
     1981 ggtcgtccca tgtcagaagt taccgttccg cactatgcag tttacaatgg tgtgaagcaa
     2041 gatattttct gtattgagcc aggggtgccg atttacaatg agttcactcc tggttatgag
     2101 aaaaatccat tgcctgatat gtcggagaaa gcgaaattag tttccgtttt atggaaggaa
     2161 gctggtacgg atgtggatac acatattgtg gcccagaaga tgatttggca agaagttaat
     2221 ggctacacgc ttcactcaat taaacgttct gatggtagtg cagtaaatat cgcagccatt
     2281 gagtcaaaaa tcaatcaagc cattgcggat tatcaaaaga aaccaagctt ccataactcc
     2341 acagtgaaaa ccgtgttagg tcaatcgact actgtgacag atacgaacgg cttgaattta
     2401 tcggagtttg atgaagttgt cgagaatatc gcaaatattg attatcgtgt gaacggcaat
     2461 caactagtga ttactccaaa tgccaattca aaagagagtg gtgaattaac cctaaagaag
     2521 tcagctaata caggaacacc ggtagcttat aaaatggctg gtcaacaaac tttgatggcg
     2581 ggggcaattg ataagcccaa tacctacaca atcaaaattg atgtggaaac tgagggttct
     2641 ttgaagatta aaaaagtcga taaagaaaca ggaaatgttg taccgggaac cgttttccat
     2701 ttagactttg gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga aggcattgcg
     2761 acattggatg agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc agtgccagct
     2821 ccttatacga ttgacactac accaatcacc gcaaccatta aatctggtga gactatttct
     2881 gtgacttcaa aaaatactcg cgaaaaaggt caaatcattt tgaataaaat gggggtggaa
     2941 acaggaactg atctttggaa cgacaaatac tcattagccg gaaatacgtt tgccattcgc
     3001 aaagacagtc caaccggtga aattgtccaa gaaatgacca cggatgaaaa aggtcatgcg
     3061 gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctattatgt gaccgaaacc
     3121 aaagctagta atggctttgt gaataccttt aaaccagtaa aagtcgaatt gaactatgtc
     3181 aatcaaaccg tggctcttgt taccagtaac gtaaaagggc aaaaccaaga aatcactggt
     3241 gaaaccactt tgacaaaaga agacaaagat accggtaatg aaactcaagg taaagctgag
     3301 tttaaaggag ctgaatatac tctctttact gcaaaagatg gtcaagcggt caaatggagt
     3361 gaggctttta aaccagagtt agtgaaggga acgaaagctt ctgatgaaac ggtgactttg
     3421 actttagatg aaaagaatca agttgctgtt aaacacttag cgattaatga gtattactgg
     3481 caagaaacca aagcacctga aggatatacc ttggatgaaa caaagtaccc tgtttccatc
     3541 aaaaaggtgg atgataacga aaaaaatgcc gtgattaccc gagatgttac ggcaaaagaa
     3601 caagtcattc gctttggctt tgatttcttt aaatttgctg gatcagctgc tgggactgcc
     3661 gaaactggct tcaatgatct gacctttaaa gtatcgccat tggaaggaac cagtgagatt
     3721 acagatgccg aagatgaagc aattacagct tataacgagc aactaggttt tgatggctat
     3781 ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga ggctccagaa
     3841 ggatttcaaa aaattacgcc attagaaatc cgttctactt ttaaagaaaa taaagcggat
     3901 tacactaaga gtgaatacat ttttacgatt accgaaaaag gacaaaaaca accaattaaa
     3961 acggtgaccg ttccttacga gaaactgacg aataaagcat tttctgttag cttgaatcgt
     4021 ttgatgctct atgatttacc tgaagaagaa gatagcttaa cttctcttgg gacttggaaa
     4081 gacggaggca aagaattgac agaccttgat tctaccgagc tagttgataa attgagctat
     4141 aacttgcatg aaatcaaaga agactggtat gtggtagcgc aagccattga tgtggaagcg
     4201 acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc cgaaacaacc
     4261 gcaacgttgg cgaataaaga gaaaacagga acttggaaaa ttttgcataa attaaccgca
     4321 gaacaagttt tggataaaac catcgtctta ttcaactatg tgtatgaaaa caagaaaccc
     4381 tatgaagcag gtgatgatcc agtagcgaaa gatgctagct tgaacaatca agcccaaact
     4441 gtcaattgta cggtggaacg ccatgtttcc atccaaacaa aagcccacct agaagatggt
     4501 tcgcaaacct ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc gattacccat
     4561 gatgtactgg atggctcaaa agaagctttt gaaacaattc tgtatgcatt acttccggat
     4621 aatacgacca aagaaatttg gaaatctggc aaaattgatt atgaagtgaa tgacaaagaa
     4681 ttcactaaaa ccgtacttgc ggaaaaagtg gacacaagta aatatccaga aggtacaagt
     4741 tttaccttca aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa acacaatgag
     4801 gatctaaaag aaaaaactca aactttaaca cctaaaaaag ttccgacaac accaagtgca
     4861 ccagaacaac cggaaatacc aactgttcca agtgactctc aagaatctag tcctacagta
     4921 aagacattcc cacaaacagg ggagaaaaat tctaatgttc tgctgtttat tgggtttacc
     4981 ttgatctttg cgactgctgg gtattatttc tggaatcgcc gaaactaagg tgatgtgatg
     5041 aaacaactaa ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag tggtcgcgct
     5101 atttttgtat ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg aaatttgttg
     5161 tgccagatat ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc gaagttttaa
     5221 ctgaaggata tgggcggaat actacagtaa ttggtcgtag ttatcatttg tattccagta
     5281 agcaacgagc cgatgatatt gaagtggtgg tagctgcgga agccggcgaa aaggattttg
     5341 atcaagatca accactaaaa gccgtgaatc cccatttggt tgccaaaggc tatgagattg
     5401 agaatcgtgg gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa gcataggagg
     5461 aaaagaagat gagattaaca gaaggtattg ttgtagattc aggactaacg tttgggaaat
     5521 tacgattttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga acggttagta
     5581 acgaggtaaa agaacggact tataatttga aatcctctgc gcaaggtcga atgatccaag
     5641 ttagtatccc agcaaatgta cccttacgcg agtttgccta tgacgcagag gtagagttgg
     5701 tcaatccaat catggatacg gtcgccaatt atgttttccg agaaggaact actgtcaatt
     5761 ggttcattaa ggccgatgat ttagtcttga aacgacaacc agatcaggga aattctacaa
     5821 accaaaatga aggaaagaaa taggtgaagc gtatggaaat taatgttgaa catattttag
     5881 attgtctcga tcaatatgga aaaggtgagc taacggagga acaactcact aaagcattaa
     5941 cctatgatga aaaaatgttt ctgattatgc atcaagggtt actggaagta ggtaacgatg
     6001 ccgaagaaga ttttgatgta ctgaattggt taggggaaga agagtccttc tttatggtta
     6061 tcgaagtaaa tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa attggtgtgg
     6121 aaatgccgga tgccattgaa gtttttttaa agcaactagt ggaaacgaaa caactcccta
     6181 tagcagtaaa tgactagcat aactgcttga aattggatca atgaaatcct atgaagcttt
     6241 tttcgattgg aggtggaaac cataaaaatg tacaaagggc atcgtatacg ggcaggagat
     6301 cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg
     6361 agcgtctttt attttcaaga gcttagacaa tttgatattt ctaagctatc actttctact
     6421 atagaaatcg ttcgttctat aaaagattta atctatttgc taggaagtct cgttctttca
     6481 ggaagcacga tgttgcttta tatacacttt tttcaggatc attggcgcag tttgtggcat
     6541 cgacaaaagc tagcccggat gattctagaa aatcattggt acgaagtaaa acaaactcag
     6601 agtgaaggat ttttcaaaga cctcaatagt agtcgaacca aagagacaat cagttacttc
     6661 cccaaaatct attaccgtat gaaggatggt ttactttcta ttcgcgttca aatttcactg
     6721 ggaaaatatc aagatcagct tttaaagctg gaaaagaagt tagaaagtgg tctatattgt
     6781 gaactagtag aaaaggaact caaagactcc tatgtggagt acactttact gtatgatata
     6841 attgccaatc gaattgggat agacgaagta gtggcagaaa gtggggcttt gcgattgatg
     6901 aaaaatcaag tatgggctta tgattcttta cctcatatgt taatcgctgg cggcacaggt
     6961 ggtgggaaga cttacttttt actcactatc atcgaggctt tattaaaatc tgatgccgag
     7021 ctttttgttc ttgatcccaa aaatgcggac ttagccgatt taggtacggt aatgcctcat
     7081 gtctattccc aaaaggagga aatttctgct tgtgtggaag atttctatga gcgcatgatg
     7141 actcgtagca aggcaatgaa agaaatgccc aactacaaaa caggagagaa ttatgcgttt
     7201 cttggacttc caccaaactt tttaatcttt gatgaatacg tggtttatat ggaaatgtta
     7261 acgacgaagg aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttaggtcgt
     7321 caatctggtt tctttctgat tctagcttgt caaagaccgg atgcgaaata tttaggagac
     7381 gggattcgcg atcaatttaa tttctgggtg gctttgggtc gtatgagtga actgggatat
     7441 tccatgatgt ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa aggtcgaggc
     7501 tatgtagata cgggagggag tgtgattagt gagttttata cgccacttgt accaaaagga
     7561 tatgatttct taagggaaat tagcgacatt ggtggattaa acgttaatac ggaaggataa
     7621 aataatagta tgtagcctag gagtgtcaag aatcctgggc tttttttacg aggtgattta
     7681 tgacattttt agatttattc gctgggattg gtggcttttg cttaggtatg gaacaagcgg
     7741 gtcaccagtg tatcggcttt tgtgaaattg atgattttgc tcgccagagt tataaggcaa
     7801 tccatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca gacgagttta
     7861 ttcaatctct cggaccagtg gatatccttt gcggtggatt tccgtgccaa gctttttcaa
     7921 ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt gaaatcgctc
     7981 ggttcgccgc tattctccaa cctaagtttt tattccttga gaacgtccgg ggattactca
     8041 atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg
     8101 atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt gagcggatct
     8161 tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc attggatcgt
     8221 ctccaacatc tgatcaaaac atcagaaact tattaaatat caacccttcc aatcgaggaa
     8281 tggggggaca agtctatggg tcagatgacg tagcgcctac cttgaccggt gatgaaggaa
     8341 tcaaaatcgc attgccggta gatgatggaa tttctgtagc gggtatgtta ctgggaaatt
     8401 ttgaacaagg caatcgggtt tatgagataa ctggaacagc gcctactttg tcaacgaaac
     8461 aaggcggaac gaagatcatg cttcggaaaa acgaaacaga atatcaagag gtgaagccag
     8521 ggaacagtgt gaacctggct tttcctaatt ccacaagtcg tcgaggccgt ctgggaaaac
     8581 aaagtgtgca taccttatta acaggagacc aacaagcagt cgtcacggat cagtatcaaa
     8641 ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccggac tgggcttttg
     8701 atcgagcttc tcaagtcaat tcagatagtc agttgtataa gcaggctgga aattctgtaa
     8761 cagttccggt catttttgat attgctagaa gattaaagga ggtaaaaaca gatgatttat
     8821 gatgagttga ttggggaatc tactgggtga ttggcaagat tcagtcagat ccggaattgg
     8881 aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa gtccctgatg
     8941 atccgtatgt aattgatgag gaaacggatg ctcgaatgga gctgaatcaa gtcatcagtg
     9001 aattggaacg aattgcggac tttgctaaag aacctgatat ccgccaatac ttgtttgaga
     9061 taaaagcaga gttggaaatt gagggtatta ccgcggagta acggccaaat aaaccactga
     9121 taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg tgcctgttta
     9181 tggacggcgt tagccggcca tgatgtctca ccccccgtat ctaacagggg ggtacaaaaa
     9241 cacaaaaaat aagcaagcaa cgataaggaa tggctgtcct atcaaggata ataagcaatt
     9301 tgtgtagatg ttaactgatt tgaaaaagtt aacaccttaa atttggaggt gagaacttgg
     9361 cacaaaggaa tttagattat cggctattaa aagaccggcg taatgaatat ggcgtttcac
     9421 aaaataaatt agctacgact tgtggactta gtcgcccgta cttaaatcag atagaaaatg
     9481 gtggcgtaac cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg gaaagtttca
     9541 atcccgagtt acctttaacg ttactgtttg actatgtaag gattcgcttt cccacaacgg
     9601 atgcgaggaa aatcattcaa gagattctcc atttgaaatt tgattatatg ctccacgaag
     9661 attacgcctt ttactcctat caagaacaat atgttatggg agatattgtg gtgatgttgt
     9721 cacatgaaga agataaaggc gtccttttag aattaaaggg tcgtggttgt cggcagtttg
     9781 aaactttttt actcgcccaa aagcgtagct ggtatgactt tttcgaagat tgtctaaaag
     9841 ccggtggcgt gatgaaacgc ttagacctgg caatcaatga tcgagttggc ctgttagata
     9901 ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttca
     9961 aaagctatcg ttccggggaa ctgttgaaac ctgatgataa agatggtatg ggcaatactt
    10021 tgtatattgg cagtcttaag agtgaggtct atttctgttt gtacgaaaaa gactatgagc
    10081 aatatatcaa gttagggatt cctttagacc agaccgaaac gaaaaatcgt tttgaaatcc
    10141 gactgaaaaa tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt cgcagtattg
    10201 aaagtaccac attttccatt attaaccggt acttgcgatt tgcggataag gtggaaggca
    10261 aaaggcgaac taactggccc ttgaatgaac aatgggggcg cttcattgga cggaatcaga
    10321 aggaaattca attaacttct gaaccgaagc cttataccat tgaacgaacg ttgaactggc
    10381 ttggacgaca agtcgcccct acgtggaaga tggccaagga gttggatcgg ttgaatcaga
    10441 caacgtacat tcaggatatg gtaaataata ctcgactatc agaccggcac aaaaagattt
    10501 tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa tcgtgatgga
    10561 aattgtgaaa gatattttat tggtgtttgg tggcagtatg cttggtgtta ctgtgatgtg
    10621 cttgatgcat gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa agtgatgaaa
    10681 tgaatttcgg tcaaaatttg tatcaatggt ttttaaccaa tgctcaatct ttagtcttac
    10741 tagcgattgt ggtcattggt ttgtttttag gatttaaacg cgagttttcc aaactgattg
    10801 gcttcttggt gattgctttg attgcggtag gtctggtctt taatgcttct ggtgtgaagg
    10861 atgtcttact caatttgttt aaccgaattg tcggtgcctg agggggaatc acaatggaac
    10921 agatacgtgt ttatattgcg aaccttggaa aatacaatga aggtgagttg gtgggggcat
    10981 ggtttacccc acccgtcgat tttgatgaag tgaaagaacg gattggatta aatgacgaat
    11041 atgaagagta cgcaattcat gattatgaac tgccttttga gattgatgag tatacaccaa
    11101 ttgaggaaat taatcggttg tgtggtttag cggaagagct taaaggaacg ccaattggtg
    11161 aggtcgcttc agagattcaa catgccttct tcaattcttt cgaggaaatg gtagagcatg
    11221 tggatgatat catctattat cccgattgta atgacatgag tgatgtagcg tattacttga
    11281 ttgtagaagc tggtgattta ggcgaagtac cagaacactt gaagacgtat attgattttg
    11341 atgcgtgtgg tcgagacttg gagattggcg ggaattattt ggttacgagt cgaggggttt
    11401 ttgaactaat gtaaatcatt tgttagaatt gaccagaaat ttctatcgta cttttacttt
    11461 tctatttgat atactgaaat agaaaatatt tgttgatagg ggtgttacta atggttggca
    11521 ataagtggta caaatgtgat cttcatattc attcaactgc gagtcagtgc tttcaggata
    11581 aaaaggttac tgctaaggaa tgggtagaag aatgtttaaa caaaggactc gattgtgttg
    11641 cattaactga tcacaataca ggggaaaata ttgatgaata taagaaagtg gctatcgaaa
    11701 atggcttaga tttttttcct ggagtggaat taacgtgtgg agaaaatgga actcatttac
    11761 tagtcctatt tgataaaagt gcatgtacaa atgatatctc tgatttctta accaagatgg
    11821 gtataaaaag agaggattgg gccagcgctg cagcttgctc aatgaaatca gttttagaag
    11881 ttattgattt tgctgagcac gagggcttgc tcgttattcc tgcacatata gatgagttta
    11941 atggaatttc ttcagcaaaa tttggaaatc taggaaagat atttgaatca gaaaatatat
    12001 atgcagtaca gacggttcaa aaagaatttt ttgaaaatcg ctcacaaatg attccctcat
    12061 ctaagcgtga cactatctat gatagtgtga atgccagata tgatggtaga gttggaaaag
    12121 atactttgga atcttggtat aaatcagttg ttgaggcaga aaaaaataat atgacatttc
    12181 ttagcttttc tgataaccca cactcgaaag gaaattcaaa acatggactt tggggaatag
    12241 gcacaagata ttcctatata aaaatgaagg acgaacctga cttgggatca cttcgcgatg
    12301 ctttaatgct aggcgaaact cgagttcatt ctgatttttc taatttctcc attaatgaaa
    12361 atgaagtttt attggaaaaa ttaagctttt ctgggaccac tcttagcaca aaagaagttg
    12421 tagtcgaatt cagcgataat ttaacttcaa ttattggagg aagaggaaca ggcaaatctt
    12481 gtattacaag attcttggtc tatgtccttg gaaaggaagc agaacttgat caattttctg
    12541 aaatacaaag tgattatcag aactttgcac aaattgaaca taatggctct ggaatatttt
    12601 tgaaggatac aattgtgaaa ctgaatattt tctataaggg gacaaaatat caaataataa
    12661 gaacacagga tagacattct atttatgaat ttacacaaga gaatggtctg gttgaaaaag
    12721 aaacagaacg gttgagaatg atttccgata aggtgagtat atactctcaa aaacaaattt
    12781 atgaaatttc aaaaaatcaa actagtatac tagaattact agatggatat aactctgatt
    12841 tgatttcaga gtataaaaat gagattgaaa catgtgtaaa cgaaataaga aaattaaatt
    12901 gggatattgt ttctgtgaaa aaagaaatac aagataaggc gaaggtagag cttgaaattg
    12961 aagatttaaa aaaacaagta gaaaagctat ctcataagag ttataaagat gtttatgatg
    13021 agtattcaaa agagacagat atttataggg aactgaaacg tgatgtggag gcacttaagg
    13081 aaattcccaa aaatctaact gatagtgttg acaaaattga ctttggcaat ggtataaaag
    13141 ttacagacga aattgatgaa caccgcggag aattgattaa gaatttagtg accaatcggg
    13201 ctaagattca aacaataatt aatgaaatgt cagatgaaat tgatagttat agagcagagc
    13261 tgaataaatc agattggaaa gttaactata atgaggtttc aaagaggtat caaagaggta
    13321 tcaaaaactt agtgaagagt tatctcaaga tgaattaaaa atgttacaaa accttgattc
    13381 tttatatcaa tctttatcaa aaactgaaga gcgattggaa gaaattattc aacaagaaat
    13441 aaagcttaaa gattaccaag ttgaggttga ctcagcaatg tcaagaatga ataaatcata
    13501 tcaaaatttc tattctgaga gaagtagttc ctcgcgcgaa attttttctg atattacagg
    13561 aatagaggct actattcaac caaataggga tttcaataat tatgttaaag agctcagagc
    13621 ttgtattaat aaggaggaca cttttgacag tgagtttgga tctcttattg ataagctgac
    13681 aaaaaattcc attactaatc aagatatata taatgacata cttgatataa gagttagtgg
    13741 ggaaagtagt attttttcag ataaaaaatt aatcaattct ataagaaaat tgaatgatgc
    13801 acaagttctt gaattacaaa cccttaagcc aaatgattta gtagttatcc gacttgaact
    13861 aaatggaaaa aaagttagtt tatcgaatgc tagtgctggt caaaagacat ctgcaattct
    13921 ttctatgatt ttggcgtatg gaaattcacc actcatattg gatcagcctg aagatgattt
    13981 ggatagtcaa ttaattaata acttaatcgt aagaagtatc attacaaaaa aggaaaacag
    14041 acaaatttta attgtaactc ataatgcaaa tatcccagtt aatggtgatt cagaatggtt
    14101 gattagcatg ggagattcaa aagaaatatc ggctgatcag tgcgggtcgg tagatgaacc
    14161 caatatcaag ccaagaatat gtactgtgat ggaaggtggc gaggatgctt ttacaaatag
    14221 agcgaaacgc tatggtttta aacaaattgt agaataacac tagagggtga gacatattat
    14281 tatgtccgcc ctttttagga ggaaaaatga aaaaaataaa aagctatacc agtatctggg
    14341 cagttgaaaa agtcatttac gcaatcaatg attttcagtt gccttttcct gtgactttta
    14401 accaaatggc ttggttcgtt ttatctttac tgtttgtcat cgtatttgcc catgtgcctc
    14461 cactatctat gatagaggag gtctttttaa aatacttagg cataccggtg gctgtcactt
    14521 ggtttatgtc gcaaaagacg tttgatggca agaaaccact gggattttta cgatccttta
    14581 tcagctatca cttgcgattt aaggtgactt ttgcaggaaa gaaagtcaaa gaacagaaga
    14641 aacgatggga tgaacccagt actcttgtaa ggagtgtgaa ctatgtatcc gattaagtat
    14701 attgaaaata atctcgtctt taaccaagaa ggcgagtgtt ttgcctacta tgaattagtg
    14761 ccttacaatt actccttttt gtcacccgaa cagaaatttc aagtgcatga taactttcgc
    14821 caactaattg cgcaaaatcg tgaaggcaag atccatgcgt tacaaattgc cacagaaagt
    14881 agtattcgcg ccacacaaga acgatcaaaa aaagaaatta ccggtagact caaagaagta
    14941 gcgaaacaac gaattgatat acagacagat gcattagtat ccatgattgg cgatagtcag
    15001 attgattatc gcttttttat tggctttaaa ctaattgcga cagatgaaga agtcaatcta
    15061 aagagtctca agaaatcttt cttttctggg cttcaagaat ttgtctatgg agtgaatcat
    15121 catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgctgcta ttctaagctt
    15181 gaaaaattaa tggaaagcaa attggctcga agatttaaag tcaggcgagt cacaccgagt
    15241 gacctgacgt atttgattga acatatttat ggcgaaaaag ggataccgtt tgaagaatac
    15301 gaatttcagc taccaaagaa aaagctaaaa tcagaaacac tggtgaaacg ttatgactta
    15361 ttacgtccaa atcgttgttt aattgaagaa aaaccaaggt gcttacggat ggaacatgag
    15421 aatcatgagt cgtacgtagc gtatttaaca attaatacaa ttgtggggga gatggaattt
    15481 ccttcctcag aacttttcta ttaccagcaa caacaattta cattcccaat cgacacaagt
    15541 atgaatgtag agattgtgac gaataaaaag gctttagcta ccgttcgtaa taagaaaaaa
    15601 gaactgaaag atttagacaa tcatgcttac caatctgaca atgaaacgaa ttccaatgtc
    15661 ttagatgctt tggattccgt ggatgaattg gaaaccacct tggatcagtc aaaagaatct
    15721 atgtacaaac tgagttatgt ggttcgagtg agtgccgaat cagtggatga actaaagcga
    15781 cgctgtgatg aagtgctcga tttttatgat gataccaatg taaaactagt ccggcccttt
    15841 ggcgatatga tggggctaca tgaagaattc ttacccttaa gtaaacgata tatgaacgac
    15901 tatattcagt atgttacgtc tgattttttg gcggggcttg gttttggggc tactcagatg
    15961 ctaggagaat tagaagggat ttactttggc tacaatgtgg atacaggtcg taatgtttac
    16021 ctgaaaccgg cattggcttc acaaggagtc aagggttcag taaccaatgc gctggccgcc
    16081 gctttcctgg gttctcttgg tggaggtaaa tcctttagta acaatctctt agtctactat
    16141 gctgttttgt tcggtggtca agctgtgatt gtcgatccaa aagggggtgc gaaacgttcc
    16201 tacttgaaaa gagtaggcac agcacttcat taaatcaata tttaatgtgt gttggagaac
    16261 taaacacacg aatagtcgaa tgacggggta acgccctgaa aggctacttt aatactctga
    16321 ctggcagaaa cttgagtaaa caaggctctg tcagaagctc ggtgaagtcg gcagacagac
    16381 accgtaacag ataatgctga cgaaagattc ccagaggtgg gcgttgtgtc ctatatgccg
    16441 gaagtctata aaatatctat ggttagaatg tagatgagag gtctactgac gaatccttga
    16501 atgtacaggt ctatatcggg gctggataga aatgtccagt gacacaaaca gtgtcgctac
    16561 tgtgggtgag taagactccg catttatgaa aatccatata acgttaaagg cgttatcaag
    16621 gtagcaggct tatagaggaa acctaaggat atatgtacag atagtgtgtt tggaacgttg
    16681 aaagctgaca acatggagat tttactgtct gtgaagtgtt cacatcagaa agttattgtg
    16741 agattagcaa tgatataagg tgtgttaatg tcagtgagag tggtggcaca gtacctatga
    16801 aaccgtgata acaagcggtg gagggatagc cactagtcat atgaagaata gaattactaa
    16861 gaaaaatggt atgggttcga gtaagactaa gagatgtact tgctcctgca aacaaggagg
    16921 gtacagacca tgctaaagaa aaacaagctc cgctataacg agtattatga tatgcaacat
    16981 atttatgacg agttatacgc acagagcaag aacggtaata acttttataa gttacttgaa
    17041 attattggtt cggaacaaaa tatttgttta gcttaccgta atctgaaatc aaacagtggc
    17101 agtaaaacgg ctggaacgga tggaatgacg atagacgata taaaacaact cagcaatgct
    17161 gagattgttg ctaccgttcg ggaaagcctt agcaactacc gccccaaatc agtcagacgt
    17221 gttttcattc caaaagctgg gagcgataaa atgcgtccat tggggattcc gtgtatctgg
    17281 gacaggctgg tacaacaatg tatcctgcaa gtcctagagc cgatatgcga accaaagttc
    17341 cataatcatt cctatggatt ccgtgcgaat cgaagtgccc accatgcagt cagcagagta
    17401 acgacactga taaatttatc aaaatatcat tactgtgttg acgtagacat taaaggattt
    17461 ttcgataatg tcaatcatgg aaaactatta aaacaaatct ggacacttgg tattcgggat
    17521 aaaagattga tttgcattat cagcaaaatg ctgaaagccg aaattgacgg cgagggtgtc
    17581 ccagaaaaag gaacgccaca aggcggtctg ttaagtccac tgctctccct catagtcctt
    17641 aatgaattag actggtgggt aagcagtcag tgggaaacat tccagccgaa gaaccgcagt
    17701 aaaaatgggt ggcttcaata cgcaaagaaa tataccaaac tgaaaagcgg ttttattgta
    17761 cgttacgctg atgattttaa aatcatgtgt tcaacctatg gagaagcaca aagattttac
    17821 cacagtaccg tagattttct taataaaagg ctgaaattag aaatcagtcc agagaaatca
    17881 aaggtagtaa atctgaagaa aaattcatca gattttctgg ggttcaaaat caaagtcatt
    17941 ccaaaaggaa gaacgaaaca cggttatgtt gccaaaacag atatgaacca aaaagcatta
    18001 aagaaagcga aaacaaatct aaagttaaag gtaaaagata tagccagaca tacgactggc
    18061 ttcaatatca gcagatataa tctgacagtt atcggtatgc agaattacta ctgcatagca
    18121 actaacgtat ataacaacct gacagaagta agttacgctc tcttaccgac catacgaatc
    18181 cgtttaagga acattgcaaa atcagttcca tttgaaagta caagttcaga atttcagagc
    18241 aggacaaaag gaatcagacc aaaaacaaaa atagtgatga ttgcggataa tccgctattg
    18301 ccgattcaag gagtacaaca caaaaatcct atgaatttct cgcaagatat ttgtaatttt
    18361 acaaagcaag ggagaaacaa ggtgcatgag gacgttgtag ttgtaacaaa agaagaaatc
    18421 cgagcgttgt tggaaaatga aaaccctgcc gatagcgtag agtttaacga caatcgtatt
    18481 tcggcatata tcgcacaaca aggcaactgc tatgtaatga atcgtcgagg aactccgtcc
    18541 actctgatat gtattcacaa atcagacaaa ggagataacc ttgacagata ttctaatctg
    18601 gcatttgtgg aaattcccat tgcaaaggca atcctaacag aatcagcaga cgaagcaaaa
    18661 tcactgttaa aaggttatgt tcttaatagt cagcagaaga aaaaactgaa cagaatacgg
    18721 acgaattacg gataccagac aataactggg aaataactta taaatctatt tggatatgat
    18781 ggaacgccgt atgaagcgaa agtttcatgt acggtgtgaa gcgggggaaa acttggagat
    18841 aacatcaaaa agttacctat cgctatagcg aggagggtgg aaagaaacct tgccagaaat
    18901 tgcggatgaa atcagtatcc tcaacttgac gagtaagtca gaaaatcaag gtctgcttga
    18961 tccttatgtg attatgaaga aaaagaaaga ctcggaaagt ttagcaattg atattctgac
    19021 ttttttaaca ggaatttcaa gtcgagacgg ggagaagttt cctgtgttac gtaaagccat
    19081 tcgaacggtg actcaaagtg atcaacgggg gttattacaa gttattaacg aattgcgaaa
    19141 agacgggtca ccaatcgcgg aaaatatcgc cgatcatatc gagtccttta ccgattatga
    19201 ttttgctcat ttgcttttct cagacggctc gattacccaa tcgattagtt tagataaaca
    19261 gctaaatatc attcaagtgg cggatttggt tttaccggat gcagaaacga cttttgaaga
    19321 atataccacc atggaattgc ttagtgtttc gatgctaatc gtcatttcca cttttgcctt
    19381 ggactttatc catagtaacc gagaaatctt caaaattgtc gatttggatg aggcttggag
    19441 ttttcttcaa gtggctcaag gaaaaacgtt atcgaataag cttgttcgtg ctggtcgggc
    19501 catgaatgct ggggtctatt tcgtcactca aaatagcgac gatttattag atgaacgttt
    19561 aaaaaataat atcgggttga agtttgcttt tcgcagtacc gatattcatg aaatcaagaa
    19621 aaccttggaa ttctttggtt tggatcagga ggatcaaagc aatcaaaaac gcctgcgaga
    19681 tttggaaaac g
//
LOCUS       DASJTM010000050        19455 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJTM010000050 DASJTM010000000
VERSION     DASJTM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.050"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            162..890
                     /locus_tag="U4B29_001722"
     CDS             162..890
                     /locus_tag="U4B29_001722"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN2106669.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            890..1144
                     /gene="purS"
                     /locus_tag="U4B29_001723"
     CDS             890..1144
                     /gene="purS"
                     /locus_tag="U4B29_001723"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN2106670.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1146..1817
                     /gene="purQ"
                     /locus_tag="U4B29_001724"
     CDS             1146..1817
                     /gene="purQ"
                     /locus_tag="U4B29_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN2106671.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1818..4040
                     /gene="purL"
                     /locus_tag="U4B29_001725"
     CDS             1818..4040
                     /gene="purL"
                     /locus_tag="U4B29_001725"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN2106672.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4025..5464
                     /locus_tag="U4B29_001726"
     CDS             4025..5464
                     /locus_tag="U4B29_001726"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN2106673.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5487..6539
                     /locus_tag="U4B29_001727"
     CDS             5487..6539
                     /locus_tag="U4B29_001727"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN2106674.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6536..7114
                     /gene="purN"
                     /locus_tag="U4B29_001728"
     CDS             6536..7114
                     /gene="purN"
                     /locus_tag="U4B29_001728"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN2106675.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7111..8646
                     /gene="purH"
                     /locus_tag="U4B29_001729"
     CDS             7111..8646
                     /gene="purH"
                     /locus_tag="U4B29_001729"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN2106676.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8662..9936
                     /gene="purD"
                     /locus_tag="U4B29_001730"
     CDS             8662..9936
                     /gene="purD"
                     /locus_tag="U4B29_001730"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN2106677.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10058..10972
                     /locus_tag="U4B29_001731"
     CDS             10058..10972
                     /locus_tag="U4B29_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2106678.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11105..12670)
                     /locus_tag="U4B29_001732"
     CDS             complement(11105..12670)
                     /locus_tag="U4B29_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2106679.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12663..14114)
                     /locus_tag="U4B29_001733"
     CDS             complement(12663..14114)
                     /locus_tag="U4B29_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2106680.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14128..15144)
                     /locus_tag="U4B29_001734"
     CDS             complement(14128..15144)
                     /locus_tag="U4B29_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2106681.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15364..16452
                     /locus_tag="U4B29_001735"
     CDS             15364..16452
                     /locus_tag="U4B29_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN2106682.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16506..18032
                     /locus_tag="U4B29_001736"
     CDS             16506..18032
                     /locus_tag="U4B29_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN2106683.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18034..19239
                     /locus_tag="U4B29_001737"
     CDS             18034..19239
                     /locus_tag="U4B29_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2106684.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         6224 a         3302 c         4451 g         5478 t
ORIGIN      
        1 gattttcgtt tctgataaaa cacgaacatt aaaagaatct aatgccttaa atgtacatat
       61 ttttgttgat ttataaaaag atgattgcta aaatttaaag tgaaaaaacg aacaaaaaac
      121 gatgtatttg cttttaatgt aaataaaaag gagagacgta catggagaag aaagaacttt
      181 tgtatgaagg aaaagccaaa aaaatatatc ttacttctga tgagaacttg atttggatag
      241 aatatttaga tcaagcaact gcgttgaatg gcgcgaggaa agaccagata agtggaaaag
      301 gagaattgaa taatcaaatc acaagtgcaa ttttccgtta tctgactcaa agaggaataa
      361 aaaatcattt tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa gtacaaatta
      421 tccctctgga agtggttatc cgaaatattg cagcaggaag tttctcaaaa cgtctaggtg
      481 ttcccgaagg taaagaattc atcaaaccaa tcattgaatt ctactataag gatgaccagt
      541 tggatgatcc atttatcaat gaagagcata tctatttctt acaacttgca aagccggaag
      601 aaatcagtga gctgaaagcc caagcgttgg ctattaatga aattttgacc gaattatttg
      661 ctaaggtcgg tttggtttta gtagatttca agctagaatt tggccggtcg aatggacaaa
      721 ttttgcttgc ggatgaagta acaccggata cttgccgatt atgggatgaa cgtacgaatg
      781 aacatatgga caaagacgtt taccgcagag atctaggtga tttgatccca gtctatcaag
      841 aagtatataa ccgtctatcg gaactaactg aagaaaagga gcgagtatga tgtatttcgt
      901 aaaagtatat gtaacctata aagattcagt gttagatcct caaggggagg cagtaaaaaa
      961 agcagtccat cgtatgggat atgaaacaat cgaagatatc cgaataggaa aatactttga
     1021 gatacaactt gctgatgcag aagaaccaat cgatacagtg attgaaacga tttgtgacaa
     1081 gctgcttgca aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa tggaggaagt
     1141 ataacatgaa atttgcggtc atcgtttttc caggttcaaa ttgtgacgca gatatgttat
     1201 gggcagttcg cgagatcatg ggcgctgatg ccgaatttgt acggcatgat gagtcctctt
     1261 tagaaggatt tgatggggtt ttactgccgg gaggattctc ctatggtgac tatttgagat
     1321 gcggtgcgat tgctcggttt tctacaatca tggaagaagt gattcgtttt gctgaagaag
     1381 ggaaaccagt attcggtaca tgcaatggat tccagatatt gacagaagca ggacttttgc
     1441 caggtgtact gagacgaaat gagtctttgc attttatttg caaaactgta ccattgaaag
     1501 tcaaccccaa gacggccttt acttcagagt atgaagccga cgaaataatc tatcttccag
     1561 ttgctcatgg ggaaggaaat tatttctgtg acgaagaaac acttcaagaa ctaaaaaaaa
     1621 ataatcagat tgcctttact tataatgaca acatcaacgg cagcgtggaa aacattgcag
     1681 ggattacgaa caaagcaggg aatgtgttag gaatgatgcc tcatccagaa cgtgcagtag
     1741 aagacttgct aggatcagaa gatgggaaac ggttctttgc ttccatttta aaaaacttcg
     1801 gaaaggtgac cgtatagatg aaaacattga aagaaccgac agctgaagag atcaagaaaa
     1861 aaagaatcta tgccgaatgg ggattgactg atgaagaata tcgaatgatc gaagaagata
     1921 tcttaggaag aatgcctaat tatacggaaa ctggactgtt ttctgttatg tggagcgaac
     1981 attgttccta taaaaattct aaaccggttc tacgcaaatt tcctactagt ggacctcatg
     2041 tgttgcaagg ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga ctagcagttg
     2101 tcttcaaagc agaaagtcac aatcatcctt cagccgtgga gccttatgaa ggagctgcga
     2161 caggagttgg cggaatcatc agagatattt tcagcatggg ggctcgtcca attgcgttgc
     2221 tagattcatt gaggttcggc gaattagata atccaagaac gaaatattta ctagaagaag
     2281 tagttgcagg tatcggcgga tacggcaatt gtatcggcat accgactgta ggcggcgaaa
     2341 ttgcatttga tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc gtaggtttga
     2401 ttgaacacaa acatatccag aaagggcaag ccgctggcgt cggtaattcg atcatgtatg
     2461 taggtgcaaa aacaggtcgc gacggtatcc acggagcaac ttttgcctca gaagaattta
     2521 atcaagaaga agaacagcaa cgttctgctg tacaagtagg cgatcctttt atggaaaagc
     2581 tgctgcttga agcgtgtttg gaattaatct tgcatcattc ggattctcta ataggtatac
     2641 aggatatggg agcagcagga cttgtttcat ctagtgcgga aatggcttca aaagctggct
     2701 caggacttat attgacatta gatgaagtcc cacaaagaga aactgggatg acaccttatg
     2761 aaatgatgtt atcggaatca caagaacgga tgttgatttg tgttaaatca ggagaggaag
     2821 aaaaaatcca agaactattt cagaagtatg atctagatgc agtcactatc ggtaaagtaa
     2881 cagacgacgg gcaatatcgg ctatatcatc aaggagaaga agtagcaaat ctgccagtcg
     2941 atgcattagc tgaagatgca ccagtttatc ataaagaaat gaaagaacca gctcgaatcg
     3001 tagaatttca gcagttagct ccttatcagc cagttatcga agaacctgga caagtattgc
     3061 ttgatctgct gcagcaaccg acgattgctt caaaacgttc aatctacgaa acttatgatt
     3121 cacaagtaca aaccaataca gttgtccgtc caggaagcga tgctgctgtg atgagggtta
     3181 gagggacgaa taaagcgtta gctatgacca cagactgcaa cgcgcgctat ctgtatttgg
     3241 atccaaaaat cggaggacaa atagcagtgg cagaagctgc tagaaatatt attgctagcg
     3301 gtggtaagcc cttggcgatc accgactgcc tgaactatgg ttctcctgat aaaccagaag
     3361 ttttttggga attatcgact tcagctgacg gaattgctgc tgcgtgtgaa acgttaggaa
     3421 ctcctgttat ttcaggaaat gtctctttat ataatgaaac ggatggtcaa gcaatctatc
     3481 caacaccaat gattggtatg gtcggattga ttgaggatca taaacatatc actactcagg
     3541 aattcaaaaa atcaggggat ctaatctata tattaggtaa gacgtttgca gattttgatg
     3601 gcagtgaatt acaaaagatg cagcttggaa gaatcgaagg tgtgatccga aactttgatc
     3661 tatccattga aaagagaaac caagagttag tattgacagc catccaaaac ggattgattg
     3721 aaagtgctca tgactgttca gaaggtggct tggcaattgc actggcagaa tcagctttca
     3781 aacatcagct agggatatct gtacagtttg aattgtcctc tgctcaatta tttgcagaaa
     3841 ctcaatcaag atttgtgttg acagtcgcac cggaaaataa gacacgattc gaagaaatga
     3901 tgggagatgc tgctgtttta gcaggtaagg tgacggatga agcgataatc gaaatttcag
     3961 caactgacgg tcagatcaaa atagaaactg cagtagcaag aaagtgctgg gaggatgcaa
     4021 tcgtatgtct tatgaagtga agagtttaaa tgaagaatgc gggatcttcg gtgtttgggg
     4081 tcatccagac gccgctagtg tggcatactt cgggctccat agtcttcagc atcgaggaca
     4141 agaaggtgca ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc gagatttggg
     4201 gctgctttct gaggtattca aagatcaaag aaaattgcag cagttaacag gagaagctgc
     4261 aatcggacat gtacgttatg caacagcagg caatggaagt gtggacaata ttcagccttt
     4321 tctattcaaa tttcatgatc agtcgatcgg cttagcgcat aatggtaact tgatcaatgc
     4381 tcgttccctt agaaaagagt tagaggaaaa tggggcgatc ttccattcta actccgatac
     4441 ggagatattg atgcatttga tccgacgaag tgaagaaaca gattttttaa ataaattaaa
     4501 aagtgctttg aatgaagtca aaggtggctt tgcctattta ttaatgacag aaacggcaat
     4561 gattgccgct ttggacccaa atggttttcg tccattagca atcggacaaa tgaaaaacgg
     4621 agcatacgtg attgcctcgg aaacttgtgc attagaagtt attggggcac gttttataag
     4681 agatgtccag cctggtgaga tagtgatcat caatgacgaa ggaatccaga ttgattgctt
     4741 tacgaaggac acgcagcttt ctatttgttc gatggaatat atctattttg ccagaccaga
     4801 ttcaaacatc gcagggatca atgtgcatac tgcacggaaa aatatgggac ggaatttagc
     4861 gaaagaatcg ccagtaaaag cagatatggt cgttggcgta cctaattctt ctttgtcagc
     4921 cgcaagcggt tatgcggaag ctagtggaat cccttatgaa atcggattag taaaaaatca
     4981 atacattgcc cgaacattta tccagccaac gcctgagcta agagagcaag gtgttcgaat
     5041 gaaattatct gctgtacgag gagtagtgga aggtaaaaag gtgattatgg tcgatgattc
     5101 aattgttcgg ggtacgacaa gcagacggat cgtccatctt ttgaaagaag caggggcaaa
     5161 agaagtccat gtgaggattg cttctcctcc tttgaaatat ccatgttttt acgggattga
     5221 tattcaaacg cgtaaagaac tcattgcggc aaatcacacg attgatgaaa tcaaagaatg
     5281 tatcggtgca gattctttag gttttttaag tgaagagggt ctgatcgaat cgatcggtct
     5341 ggaaaaagat gctccgtatt caggattgtg tatggcttat ttcaacggag attatccgac
     5401 gccgttatat gactatgaag aaaaatatca ggaatcacta aaagaaaaag tatcgttctt
     5461 ttaaaagcag accaagaagg gagtatatga attcaatggc aaatgcatac gcaaaagctg
     5521 gcgtagatgt cgaagctgga tatgaagtag tagaacggat caagaagcat gtcaaaaaaa
     5581 cggaacgttt aggtatgatg ggcgctatag gaggattcgg cggttgtttt gatttatcgg
     5641 tctttcaatt aaaagagcca gtccttgtct cggggaccga tggcgtgggg acgaaattag
     5701 cagtggcaat cgaagcagac aaacacgata caatcgggat cgactgcgta gcgatgtgtg
     5761 tcaatgatat cttagcacaa ggcgcagagc cgttatattt cttagactac ttagcaacgg
     5821 ggaaaaatga tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat ggatgtgttc
     5881 aaggaaatat ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg tactcaggta
     5941 atgattatga tctagccgga tttgctgtag ggatagctga aaaatctcaa ttattgacag
     6001 gagaaacgat caacccagga gatgtgttgc ttggaatagc atctagcggg attcattcaa
     6061 atgggtactc tcttgttcgc aaaattttct ttgagcagca gcagttttct ttatcatcaa
     6121 aactgccaga attgaaaaaa acacttggcg aagaattgct tgagccaacg aaaatctatg
     6181 tttcttcctt gcttccgctg atcaaagaaa agaaaataaa aggagctgca catatcactg
     6241 gtggagggtt tgtagaaaat atcccgcgaa tgttaccaga atctatggct gcacaaatca
     6301 aacttggctc atggccgatt cttccaatat tcggctgttt acaaaaatat ggtgaaattc
     6361 ctgagatgga gatgtatgaa atcttcaata tgggcatcgg tatggtcctg gcagtccaag
     6421 aatcgaaagt aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa gtttatgaaa
     6481 tcgggaaagt gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa gaacgatgag
     6541 aatcgctgtt tttgcttctg gaaacggaag caactttcaa gcgctagccg attatctatc
     6601 gaaaaaagga ttggaatctt cgattgactg gttgttttgt gatcagcctg aagcatatgt
     6661 tttgaaacgt gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta aagaatttga
     6721 ttcaaaaaaa gagtatgaag aagcaatttt gcataaattg aaagaaaaaa agatcgattt
     6781 gatcgtatta gctggatata tgcgaatcat tggaccggta ttgctgaaaa attatgataa
     6841 aagaatcatc aatatccatc cgtctctttt acctgccttc cctggtttgc atggcatacg
     6901 tgatgccttt gaagctggag taaaagaaac gggtgtcaca attcattata tcgaccaagg
     6961 tgtagatact ggaccaatca tccggcagga aaaagtgaga attgaacagg aggacacgtt
     7021 cgattcttta gaagaaaaaa tccatagagt agagcatcga atctatccag aagtgattag
     7081 tgaaattatt gaaaatggag gctatctgaa atgacaagag cattgatcag tgtatcagat
     7141 aaaactggga ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga aatcatttca
     7201 acaggaggaa caaagcatgc gttggataaa gctgggatca aaacgctttc gatcgaagaa
     7261 gtgacagggt ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc taaaatccat
     7321 ggtggtcttt taggaagacg cgatctccct agccatatgg aggcaatgga aacggaaaat
     7381 atccaaccaa tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac gattatgaaa
     7441 ccagatgtcg aaatagcaga agctattgaa aacattgata tcggtggtcc tagtatgctc
     7501 cgtagtgcag caaaaaacca tgaatttgtc acctctgttg ttgatccatc tgactatgaa
     7561 gaagtacttt cagaattacg tcagtatggg catacttcac tttctttcag acaaaaacta
     7621 gcggcaaaag tctttcgtca tactgcggca tatgatgcac tgatcgctca atacttaacg
     7681 gattgggtgg aagaaaaaga accggaaaaa ttgacactta cgtatgaacg aaaacaaacg
     7741 cttcgttacg gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc tcttctggaa
     7801 aaatattcga tcgcagcagc aaaacaactc catgggaaag aactttctta taataatatc
     7861 cgagatgcag atgctgcgct acggatcatg aaggaatatg acgagccaac ggtagtcgcc
     7921 ttgaaacaca tgaatccttg tggtattggg acagcatcta cgattttaga agcgtgggaa
     7981 gcagcgtatg aagcagatcc tgtttcaatc tttggtggga tcatcgtttt gaatcgagaa
     8041 gtagatctgc caacagcaga acagatgcat aagctgtttc tagaaatcat cattgcccct
     8101 tcttatacac aagaagcttt ggaattactg caaaaaaaga aaaacatccg attactgaca
     8161 gtagactttg cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt cttaggtgga
     8221 cttcttgtac aagatcaaga tctggcaata gaaaatgaag aagagtggaa agtcgtgaca
     8281 aaacgtaagc cgactccagc agaagcaaaa gcattagcgt ttgcttggag agctgtcaaa
     8341 catgtgaaat ctaatgcgat cgtactggca aacgaacgtc agacagtagg aatcggagcc
     8401 gggcagatga atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca agagaaaatg
     8461 gaaggtgcag ttttagctag cgatgcttat tttccaatgg atgattcggt tgaatatgca
     8521 gccaaacatg gtataaaagc aatcattcag ccaggaggaa gcatcaaaga ccaagattct
     8581 attgatatgg cgaataaata cgatctagca atggtcttta ctgatgttcg gcattttaga
     8641 cattagagag gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg tcgtgaacat
     8701 gcaataagta aaaaactttt agccagtccg aaggtggata cggtgtattg tgcgccagga
     8761 aatccgggga tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg agatcattca
     8821 gcgttaatcc attttgttaa gaaagaggct attgattgga gtatcgttgg tcctgaagtt
     8881 cctttgttaa atgggatcgt cgatgatttt atggcggaag gattgaaaat cttcggtccg
     8941 acaaaagcag cagctctgat cgaaggttcg aaaagttttg ctaaacaatt aatggatact
     9001 tatcaaatcc cgacagctaa atccagaagt tttgaatgtt acgaaaaagc agcagcgtat
     9061 atcaaagaaa atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc tgggaaaggt
     9121 gtagtcgtgg ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat gttgaaccag
     9181 catcgatttg gggatagtag caaaaaaatc gtcgtagaag agtttttggc aggagaagaa
     9241 ttctcccttt tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat ttcgcaagat
     9301 cataagcggg catttgataa cgataaaggg ccaaatacag gcggaatggg tgcttattca
     9361 ccagtaccgc aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt tttaaaacca
     9421 gcggctttgg gaatgaaaga actaggacgc tctttcacgg gtgttttata tgcgggtttg
     9481 attgctacga aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg tgatcctgaa
     9541 acgcaagtcg ttctttctcg gttgaagagt gactttgcgc aagtaatcga tgatttatta
     9601 gagaatcgta cagtagagtt aaagtggcag caagaaggat acaatgtagg cgttgtggtt
     9661 gcagctgccg gttaccccga agaatatgag acaggaatgg ttctaccaga ttttctggaa
     9721 gaagagcttt ctgtttacta tgctggagtt tcctcaaata acggcaaact agttggagca
     9781 ggaggacgtc tttatttagt agaagcatac ggtgagacga tcaaagaagc acagaaaaaa
     9841 atttacaccg cgttaatgga taaaacgaca gatggaacat tttatcgtac agatattgga
     9901 gaaaaagctg ccaatagcga actgatcgtg aagtaaatca gctgcttcca tttataaaaa
     9961 atacaacaaa cataatcgga agcgccgatt ttagtgaagc cttttcataa agagtgtggt
    10021 acacttttgt tgtgtatata aataaggagg agttcagatg aaaaaaattc aattaggtac
    10081 ttctgatcag aaagcagctg cagtgatctt aggctgtatg cgtatcaaca atgcggaaga
    10141 tccagtaaaa gtgattgaga cagcttatga aaacggcatt gatttttttg accatgcaga
    10201 tatttatgga aacggtgaat gtgaagaaat ctttgcagat gctctagcaa aaacatcaat
    10261 caaacgagaa gatcttttta ttcaaacaaa atgtggaatc gttccaggtg tgatgtttga
    10321 tttttctaaa aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc tgaaaatgga
    10381 ttatgtggat gctttacttc ttcatcgtcc agatacattg gtagaaccag aggaagtagc
    10441 tgaagcgttt gatgaattgg aacggtcagg aaaagttcgt tattttggcg tcagcaatca
    10501 aaaaccaatg caaatcgaat tgctgaaaaa atatgttcgt caaccattag tagctaatca
    10561 gctgcaattt ggcttgaaac atactggaat gatcgatcaa gggattcatg tcaatatgac
    10621 agatgaagca agtatcgatc atgacggtag tgtacttgat tattcccgat tgcatgatat
    10681 gacgatccaa gcatggtcac cataccagta tggatttttt gaaggtgtat ttatcgggaa
    10741 cgaaaaattc cctgaattaa accaaacatt agaagaacta gcagctaaat atcaaacgac
    10801 accaactggc ttggcttctg cttggatttt acgccatcca gccaatatgc aagtgatcgc
    10861 cggtacgatg aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa ttaatatgtc
    10921 tcgggaagat tggtacgaat tgtaccgtgc tgctggaaac attttacctt aaagattgtc
    10981 ataagcaggt caaaagggct ataaattaaa aagacagaat aacttgaaag ctgttttcag
    11041 cttcgagata ttctgtcttt ttttattgca aatggaaaga gtatcattgt acggatttcg
    11101 tttattagtt atttttgttt ttaaggaaaa gctgtgtccg atattctttt ggagaaatcc
    11161 cttgtttttt cttaaataaa taactgaaat agttagggtc attataacca atttcaaaag
    11221 caatatctga aatacgatag tctgattgct gcaataattt ttttgctaat tcgattcttt
    11281 gttctgtcag ataatcgatg aatgtttggt taaacgattg agaaaaaatc gtactgaaat
    11341 gagagggact taatgcaact tgttgagcga cccaagttaa ggacatatct ggatcggtaa
    11401 aattttcttt gataaaatcc aatgcatggt tcaaaattgg ctgatatttg ttccttgaag
    11461 gctgtgaggg cagagcagtt aagaaagaaa tgaacatata aactgtcgaa cggtaaagcg
    11521 agtcgtcggc gctgattgct aagcgttgac tggtggatgt gcaatgaacc atttcttgcg
    11581 ggattttctt ttctttcagg cagacatgct ggagtagctt agtcaagaca aataaacgat
    11641 ataatcgtgt tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg tacttagata
    11701 cttcgttttt cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag acagaatcaa
    11761 ctggaagttt cgcttcttga atcaaatcct catagctgat aatttgttct ttaagggaac
    11821 tggcggtttc aagtatcatc tgcgtgatct caaaagatgc aggtaattcg ctgattcttt
    11881 ctgcgacagg accaatggcg acagaaaaat tatttttttg tcccttttct aattcatgga
    11941 ccaacgtttg ggctacctga taactggtct cgatcacatt ttcttcaaga gaatcaacta
    12001 ttaaaaattt gatgaatcga gaggaaacag cagagaagat cacttgttga ttctccctaa
    12061 ataaaaactt caaataatca ctgagatgag cgtaatcttc aaagtctgta ctgacttggt
    12121 ttgctgctaa taatacggtc atttttttcc ctgctaaaga gcgattgaaa gattcagcaa
    12181 gtttcaatgt ttcactgatc gtaagtgagc ctttgaacaa accgttcaag aaatgatttt
    12241 ttttcaattc gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc aaatctaaag
    12301 cagttactgc tttttctatt gcttgtttca attcgctact tttaatcggt ttaagcagat
    12361 aatcatctgc ctgtacctgg attgctcctt tcatataatc gaaatcgtcg aatccactaa
    12421 taaatatgag acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa gtcaacccgt
    12481 ccatgaaagg catttggata tcagtcagaa tgatgtcggg ctgtaagtcg ataattgaag
    12541 acagtgccat ctcaccatct ccagcttcgc cgacataagt gattggataa taagaaccaa
    12601 ggacagttat ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg atgataaaaa
    12661 ctttatacat gtttgtttcc tccaattgtt ttgggagata gagggatcat cagtgtgaca
    12721 gttgttcctt ctcggtattt gctgtcaatc gtgataggtt tttgatcatt tccgtaataa
    12781 agaaggagac gcttgtttac gttgtaaagt ccgtagccga tactgaaatc tgaatcgatc
    12841 cctttggcta actctttacg tatttctgcc agtttttctg cagggatgcc aatcccgttg
    12901 tctataactt tgaattggat catctcatca attttgcatg tggtaatacg aataaatcct
    12961 ttgtgacgga cgtacttcgt tccatggtat acagcatttt caatcaatgg ctgcaagatc
    13021 atttttaaaa tagaaacagg aaaaagtgtt tctggtatat cgatcttata gtcaagcata
    13081 tggccgtatc ttatttttaa gatgactaga taatcattga tatggctaat ttctttttca
    13141 agaggaatcc agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga aagtgcatgg
    13201 gtcatagtca gagcttcttc aaagcagtct tgttcgatca aagaaataat ggcatccaaa
    13261 ctgttataaa taaaatgggg agtaatctga gcttgcaata cacgaacttc actttgtgct
    13321 aaatggtatt gtttcaaagc gttctcccgt aaagactggt ttagatcttc cgccatttta
    13381 ttcatgctag cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa acgaaagcca
    13441 aggttccctg aagaaagttg ttctgacatc tcgatcagct gattgatcgg ttcttggacc
    13501 tttctctcta aaaagcgacg attctttttc gcatagaaaa aaatggtact aagaactcca
    13561 agttccacta ttgttaatag aatgagcgag cggacaagtg tcgtattttt tttgcttgct
    13621 aagtcgatct ccacacgaac aaaatcttgt aagatatctg acagcagttg agtgacagaa
    13681 tctacctgat tgatgatctc ttcattggta tccataaaat cttttgccaa gatattattg
    13741 atcagctcgt tttgataatc ttccagtgta tcgatcgtac gtagcgatac atctaacgta
    13801 ctaatttctt taagtgtatt tgtattattt ttgatctctc gaatatcatt cctaagttct
    13861 tctaggatat tttcctcatt gaactcatct acggttactt ggccagaaat caagtcccac
    13921 atactttcca aaactttttg atcaaccttc aaagcaattg catctgcttt ttggatattt
    13981 gcgatcgtct gttgataaat caaaagctgt tgcgaatata acagtatgct cagtagcaaa
    14041 gggataacag caaatagaag catggctttg tttgcttggg caaaaatctg ttttatgctt
    14101 aaattttttg tcatgatact tctccagtca ataatttctg gttgggatgt tagctatatt
    14161 ctgctgggaa aagacagtct cggaaataaa aatatcttta ggtaatgttt ttcctgtttg
    14221 aatatttcca aaataacgag taacggtatt ggctacatac cagccgacat caggtttaca
    14281 ctcgactaca caattgacga ttcctgagcg tagcttctcg atcatatctt cttgtccatc
    14341 aatagttaca atgatcaaat cttctccagg tttgattgat gtttctttta tcgcagctaa
    14401 agcaccgtga gtcatttcat cactatggct gtataataca tcgatttcgg ataaatcatt
    14461 tgtttctaaa tatcgtttca tctggtcttt tcctttcatc cgaataaaat caccgacaat
    14521 tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt ccatcttttt ttagggttgg
    14581 tttacgattg attgattcca aaaatccttc ggagcgtagc ttggtcggtg tggagttctc
    14641 tgaaccagca agttctagga ttctgatttc ctgtttcttt gaatttgtga agtgattgct
    14701 gacaaataaa cccgcccgat ttccttctgc tttgaaactg gggccaatgt gggtgacgaa
    14761 aagctcggga tcattcgttt gaatatgtct gtcgacaagt atgacaggga tccctgctcg
    14821 ctttgcttct tttaatacag agtcccaccc ggattcttgc aaaggagtaa aaatgatcat
    14881 atccacttta taggcaatga acgtccgtat atcttggatt tgccgttcct gattcatgta
    14941 gccatttcgg tagagaactt ggtaatcttc tttttcaagc gcagtgataa ttgattcagt
    15001 atgctttttc cgccagctac tctccgttcc tgattgcgaa aaaccaacga ctagtgggtc
    15061 aggtgcttcc tttgtattag gtgtacaccc catcatttgg taagcgatta caacaagggc
    15121 taaaaaaaat aaaacacgtt tcactttaaa tcactccatc tggatttgtt agtactatct
    15181 tatcagataa aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat ccaagaaact
    15241 taaaaaaatc caagacaaaa aaagtacacc taaaaaattt aaagtaaatc ccataaaaaa
    15301 atcaatgtta acgctttctt tattagctat actcggtgta tcaattgaaa tgggggataa
    15361 aaaatgaaaa acaaatttag aaagattgtt gggactatcg ctattttgac cgcaagcatt
    15421 ctattaagtg cgtgtggcaa ttcaggatct gcagatgata gtacggggta tgtaggaatc
    15481 gctatgccga ccaaatcagc agaaagatgg atcgctgatg ggaacaacat ggtctcggaa
    15541 ttagaaaaac tgggttataa aacagatctt caatatggag aagataaagt agaaaatcaa
    15601 gtagcacaaa tagaaaatat gattacaaaa ggggtagata cacttgtgat tgcttctatc
    15661 gatggttcgg ctttaactga tgtgttagct aaagcaaaag aagcagatat caaggtgatc
    15721 gcctatgatc gtttactgat gaattcagaa aatgtcgatt attacgcgac ttttgataat
    15781 tttggagtag gtgtatcgca agcagcttat attgaagagc acttaggatt gaaagaagga
    15841 aaaggaccat tcacgattga attatttggt ggctcgcctg acgacaacaa cgcactaatc
    15901 aattacaacg gagtgatgtc tgttttacaa ccatatatgg acaacggtca gttggtcgtg
    15961 ccatctggac agacgagttt cagtcaaatc gctacgcttc gctgggatgg ttctacggcg
    16021 caagcaagga tggataatct gttaagcgca aattatactg accaaacatt ggatgctgtt
    16081 ttgtcgccat atgatccaat aagtttagga atcatttcgt cattaaaagg cgtggggtat
    16141 gggagtgaaa gtaaaccttt acctgtgatt actggacaag atgcgacagt tgctggcgtt
    16201 aaatcgatca ttgccgggga acaaacacaa acgatcttta aagacacacg tatcttagca
    16261 aaaaatacga ttgaaatgat caaagcaatc agtgatggag aagaggttcc ggtcaatgat
    16321 actgaaactt atgataatgg ggtgaaaact gtccctacgt atttagcaaa tacagtatct
    16381 gtcgataaag ataattatca agcagagctg atcgacaccg attattataa agaatccgat
    16441 ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga gagaggataa
    16501 gaattatggc ggattgtatt ttaaaaatgg cagatatcac caaaaaattt tctggaatcc
    16561 gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga aatccatgca ttgtgcggag
    16621 aaaacggagc gggaaaatca acattgatga atgtcttgag cggcttgtat ccatatggct
    16681 cctatgacgg ggaaatcttt tatgatggaa agctgtgtca atttaaagat ttgaaagata
    16741 gtgaagaaaa aggcatcgtc atcattcatc aagaattagc actaagtcct tatctttcaa
    16801 taaaagagaa tatttttctc ggaaatgaac aagcagaaag aggcgtgatt gattgggaac
    16861 tgacagaaaa gaaaacatat gaactgctga ataaagtcgg tttgtctatc gacccgaatc
    16921 ttctagtatc ccagattggc gtagggcaac agcaactagt tgaaatagct aaagcattat
    16981 caaaatccgt ccgtctgttg attttggatg agcctaccgc tgcattgaat gaagaagaaa
    17041 gcgcgaattt gctgagattg atccgagagt tgaaagagca aggtgtaacc tccatcatta
    17101 tttcacataa gctgaatgaa atcgtggata tcgcagatcg aatcaccatt ttacgggatg
    17161 gacagacgat tgagacacta gaaaatgaaa tgatcagcga agagcatatt attcgcggaa
    17221 tggtgggcag agatttagtt gatcgttatc ctgaaagaaa accggatatt ggagaagttt
    17281 attttaaagt agaagactgg acggtctatc accctcttga tcacgagcga atcataaacg
    17341 accacatcaa cttgcatttg aaaaaaggag agattgtagg aatagctgga ctgatgggag
    17401 cgggcagaac tgaattcgct atgagtctat ttggacattc ttatggaagc aagatatccg
    17461 gacgtatatt aaaggaaggg gcagagatat cagttaagac cgttccttca gccattgcaa
    17521 acggactcgc ttatgtctcg gaggaccgaa aatctttagg actgaatctg ctgatggata
    17581 ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag tcaaaaagga gtattagata
    17641 aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa gatgcgtata aaaacaaact
    17701 ccatccatca aattgtcagt agtcttagtg gtgggaatca acaaaaagtc gttttatcca
    17761 agtggctgat gacacaaccc gatattcttt ttttggatga gccaacccgt gggatagatg
    17821 ttggggcaaa atatgagatc tacacgatta tcgaagaaat ggcagcaaat ggcaaagctg
    17881 tctgtcttat ctcttctgaa cttcctgaaa tattagggat gtgtgatcga atctatacga
    17941 tgaacgaagg acgtattaca ggagaagtct cgcgagaaga tgcagatcag gaagtcttga
    18001 tgaaattaat gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa tcttacggat
    18061 aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg atctttttgg tcgctacagc
    18121 atggtgatca ttttagcggg actacttatt gcgtttcaat tgatgacgga tggtattttc
    18181 tggcgtccat tgaatataac taaccttgtt ctgcaaaata gccatatatt agtactagct
    18241 gcagggatgt tattagttgt gctcttaggt catgtggatt tgtcggtagg gtcagtgatg
    18301 gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa cccgtggatg
    18361 acagttatcg catgtcttgc agtaggcggg ctgatcggcg catggcaagg tttttgggta
    18421 gcttatgcag ggataccggc gtttatcgtg actttagcag gattgctgat gtttcgtgga
    18481 ctgacacaag ttgttctagg aggccaatct ctagcacctt ttccagctgt tttccaaaaa
    18541 atgtctacgg gttatttacc agacttaagc gaaggaagca gccttcactt cttgacattg
    18601 attttaggaa tgatcatcac gatcgtgtta gttgttggtc aatggcgaac aagaaatcgt
    18661 cggaaaaatc atttatttga agtggaatct ccaacagctt tcttggtaaa ggcggtattt
    18721 acagcagtcg ttgtactagg aataacttat atctttgcct cttatcaagg gtatccgatt
    18781 attttgatca ttcttggttt gatcgttgca gcatatgggt ttttgacaaa taaaacagta
    18841 gcaggtcgac agatttatgc gacgggcgga aatcgaaaag cggctgaact atcagggatc
    18901 aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc agctttagct
    18961 ggattagttt tagctgctcg attgaatgcg gcaacacctc aagcaggtac atcgatggaa
    19021 ctggatgcaa tggcggcagt atattttggt ggtgcttcga cttccggcgg cattggaaca
    19081 atcatgggtg cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg catgtcgatt
    19141 ttaggcgtcg gggtagactg gcagcaggca atcaaaggat tgatcttgct tttagccgtt
    19201 gtgctagact tgtataataa gaaaaaacga acaacgtagg agaacgccgg ttattcagaa
    19261 aaaagactgt gacaagacat ttgtcacagt ctttttttcg ctaaaagcga acattcattg
    19321 tttcgtttgt atagactaaa aataatatat gtttttttac cattcgtata aattctcttt
    19381 ttgctcttga tttttttgct taattgagtt aaattacata gggcgctaaa aaaagccttg
    19441 attaaattaa taatg
//
LOCUS       DASJTM010000051        19426 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJTM010000051 DASJTM010000000
VERSION     DASJTM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.051"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..237
                     /locus_tag="U4B29_001738"
     CDS             <1..237
                     /locus_tag="U4B29_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106685.1"
                     /translation="KSLVYGETTEILLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQA
                     KNEEENQPSNHTFVINNKEDLADVMTRILKGEFQ"
     gene            470..1411
                     /locus_tag="U4B29_001739"
     CDS             470..1411
                     /locus_tag="U4B29_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN2106686.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            1413..2303
                     /locus_tag="U4B29_001740"
     CDS             1413..2303
                     /locus_tag="U4B29_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN2106687.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            2346..3146
                     /locus_tag="U4B29_001741"
     CDS             2346..3146
                     /locus_tag="U4B29_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2106688.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            3161..4012
                     /locus_tag="U4B29_001742"
     CDS             3161..4012
                     /locus_tag="U4B29_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2106689.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            4213..4674
                     /locus_tag="U4B29_001743"
     CDS             4213..4674
                     /locus_tag="U4B29_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2106690.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            4700..5152
                     /locus_tag="U4B29_001744"
     CDS             4700..5152
                     /locus_tag="U4B29_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106691.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            5258..5452
                     /locus_tag="U4B29_001745"
     CDS             5258..5452
                     /locus_tag="U4B29_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106692.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            5483..5776
                     /locus_tag="U4B29_001746"
     CDS             5483..5776
                     /locus_tag="U4B29_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106693.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            5806..6246
                     /locus_tag="U4B29_001747"
     CDS             5806..6246
                     /locus_tag="U4B29_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2106694.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            6321..6788
                     /locus_tag="U4B29_001748"
     CDS             6321..6788
                     /locus_tag="U4B29_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN2106695.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            7535..7765
                     /locus_tag="U4B29_001749"
     CDS             7535..7765
                     /locus_tag="U4B29_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106696.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            7924..8757
                     /locus_tag="U4B29_001750"
     CDS             7924..8757
                     /locus_tag="U4B29_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN2106697.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            8750..10165
                     /locus_tag="U4B29_001751"
     CDS             8750..10165
                     /locus_tag="U4B29_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2106698.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            10177..11706
                     /locus_tag="U4B29_001752"
     CDS             10177..11706
                     /locus_tag="U4B29_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2106699.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            11624..12676
                     /locus_tag="U4B29_001753"
     CDS             11624..12676
                     /locus_tag="U4B29_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN2106700.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            12678..12995
                     /locus_tag="U4B29_001754"
     CDS             12678..12995
                     /locus_tag="U4B29_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106701.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            13122..13745
                     /locus_tag="U4B29_001755"
     CDS             13122..13745
                     /locus_tag="U4B29_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN2106702.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            13760..14650
                     /locus_tag="U4B29_001756"
     CDS             13760..14650
                     /locus_tag="U4B29_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN2106703.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            14673..14888
                     /locus_tag="U4B29_001757"
     CDS             14673..14888
                     /locus_tag="U4B29_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106704.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            14900..15241
                     /locus_tag="U4B29_001758"
     CDS             14900..15241
                     /locus_tag="U4B29_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106705.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            15241..15612
                     /locus_tag="U4B29_001759"
     CDS             15241..15612
                     /locus_tag="U4B29_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106706.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            15605..16000
                     /locus_tag="U4B29_001760"
     CDS             15605..16000
                     /locus_tag="U4B29_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2106707.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            16002..16379
                     /locus_tag="U4B29_001761"
     CDS             16002..16379
                     /locus_tag="U4B29_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106708.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            16380..16988
                     /locus_tag="U4B29_001762"
     CDS             16380..16988
                     /locus_tag="U4B29_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2106709.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            16988..17329
                     /locus_tag="U4B29_001763"
     CDS             16988..17329
                     /locus_tag="U4B29_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106710.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            17571..>19426
                     /locus_tag="U4B29_001764"
     CDS             17571..>19426
                     /locus_tag="U4B29_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN2106711.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIY"
BASE COUNT         7023 a         2831 c         4081 g         5491 t
ORIGIN      
        1 aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat gcaagaagaa
       61 catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc tgtggatgct
      121 tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt tgttattaat
      181 aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt tcaatgatga
      241 atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt gtagcgaaaa
      301 gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc gtgttagcaa
      361 gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt aactgatcct
      421 gattatgcta aaccaccata tgaagcacca attgatgagg aggatgaaga tgagtaaatc
      481 taccttagaa atgagccatc aagaatggct tgaagaccgt aagaaaggca tcggaggttc
      541 ggatgttgga acaattttag gattgaataa atggaaatct ccttatcaac tatggctaga
      601 aaaaacagga caagtagtac ttgaagaatc aggaagtgag cccgcttatt ggggtaatgt
      661 tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag tacgcagaag
      721 aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg atcgggaagt
      781 agtgggagaa aatgccattc tggaatgcaa aacagccaat caatttctcg gtaaagaatg
      841 ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt caacattaca tgaacgttct
      901 aaacaaagac tattgttaca tcgctgtctt aatcggtggt caaaaattta tctggaagcg
      961 gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag agttttggga
     1021 aacaaacgtt cttggaggta tcgagccaat tattgatggt agtcaagcga cagctgattt
     1081 cttgaaagag aagtatgcag atgttgaaga cgttcaaacg acgttaccaa ttcatttcga
     1141 tgaactagtt gaacagaaaa acgaactcaa acggactaag aaagaaattg aatcagctat
     1201 ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca gtatcggtat
     1261 cactcaaaaa aacatcatca gttggaaatt tgtccgtacg agacgtatga actcgaagaa
     1321 actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg ttactgaatc
     1381 aagaaggcta accgaaaggg agatcaaata atatggcaac aaatgaatcg ttaaaaaatc
     1441 aattggcagc aaagccacag aaacaagttg caccaggtca attaggactt aaagctttga
     1501 tgaatacacc aacaatgaga aagaagttcg aagaggtgct tcatgacaat gctaatgctt
     1561 ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat agtgaaccga
     1621 tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta gataagaatt
     1681 taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga aaatgggaaa
     1741 agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa cgatcaggta
     1801 aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc tggaaccgac
     1861 tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc attggatatg
     1921 ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc aaacaagaaa
     1981 tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta agtggtgtgt
     2041 gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt ctttctaaat
     2101 ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga gtccaaaggg
     2161 ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt cctgaaagaa
     2221 aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta tttgatgcat
     2281 ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct agaggagaaa
     2341 tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca ttcgtttgga
     2401 ccaatcctta tatgtataaa ttatggaact tgtgtttgat gaaagcgagt catgagaatc
     2461 gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc gtcacagggc
     2521 gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa gtgaacagcg
     2581 gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac atcaaatcaa
     2641 ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc agtgaacaac
     2701 aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag aatgaaaaga
     2761 attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa aagcgtgttt
     2821 acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag atttgtcaga
     2881 atcaggaaat caaaaagccg aatctgcaaa gttgggcaga tgacattcgg aaaatgatcg
     2941 agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt cagcacaatg
     3001 tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac gacacaatgg
     3061 cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa tacgagaaat
     3121 ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg ttggggaaat
     3181 catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg aatgcggaca
     3241 gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta agccaacctg
     3301 tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag aacgaattta
     3361 caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg ttgtgcctaa
     3421 tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag aaacaagaca
     3481 agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc caagtcacct
     3541 tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag cggcttggga
     3601 agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac aggaaatgct
     3661 ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg agggatcgtt
     3721 gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt cagaactagg
     3781 aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt tcttggatgc
     3841 acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac tgaaaaaagc
     3901 ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta cgatggtatt
     3961 ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat gaatttgact
     4021 aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga tcgtgacatg
     4081 gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct aattatttat
     4141 cgggatacag aaagatttct cactgtttta ggtaacgtct acgagaatcc agagttattg
     4201 gaggtagagt aattgccaaa aatattagat gcatgttgcg gtagtagatt gttctggttt
     4261 gataagaaca attctgacgt aacatttatg gattgtcgtc agcagtatga agaattatca
     4321 acaggtcatg tgatcaatgt tgatccagat gtggtcgcag actttcgcga tatgcctttt
     4381 gaagatgatg tgttcgatat ggttgtattt gacccgccac acttaagaca tgtcggcgaa
     4441 aactcatggc ttgccaaaaa atatggaaaa ttagacgaac tttggccaga agacatcagg
     4501 caaggttttg cagagtgtat gcgagtttta cgaccttcag gaacattaat ttttaagtgg
     4561 aacgaggaac agataccact ttctgatgtg ttagaggcta ttggagaaca gccattgttt
     4621 ggtaataaac gaagcaaaac acattggctt gtgtttatga aaaaattcag ctaacggagg
     4681 aaacaaagga ggaaaagtaa tgaagattgt attaaataaa tgttttggtg gctttgggtt
     4741 gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat tcccatacat
     4801 caataacttt agctatgact cagatgatga atacacgaga tacacaggcc aaaagctagg
     4861 ttcgatggat tttatctact atttcaaaaa agacccgaaa atcgacaaag ttacaggaat
     4921 ttatagcgaa attgaccgac tgtacggaat tgctgatgat tccagttttt caagtgattc
     4981 aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg aggccagtgg
     5041 accatatgca agtcttaaag tcgttgatat acccgatggg gctgagtggg aaataagtga
     5101 ttatgacggc gttgaaactg cacattatgg ttttcaaaca ggtagtattt agtccactaa
     5161 tgacaactaa agaggaggca gaaggatgag tgaagatgaa tactttgaac agtagtctat
     5221 taacgacaga gatttttaac tggaaagggg tgtagtcatg gacgcatcgg aagtagtaaa
     5281 taaaattaat agtctcaaag aggttttcgg agacgtcgaa gtagtgataa acgcacaagg
     5341 ctatgacaac gaatttttta agcagattac cgacattagc attcagcaag gaatggaaga
     5401 cgaagacgga aactttatcg atgaagtagc cattttagtg acttgcgagt aagtaattga
     5461 cagctaaaga ggaggaagcg gaatgaaact aaaagacgga ttttactcca gcggccacgg
     5521 catcggcggt ttaatgctag atatgccgac aaagaatcct aaaacacgta agaaaccaaa
     5581 attcaaagtc ggtgacatgg ttcgctgcga agcagaagga ttcatctatc catttcgtgg
     5641 atatgtagaa cacgtctata atcactcagc aatcattcgc attgaaaaca cgatggaatg
     5701 tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg tggacatgga
     5761 agttataaaa tcttaatttt gcttttttac aactatccta caacaatgag cgttattcca
     5821 caactggaag gagaagaggc aattgtgaac attttagaga atatagatat taagcaaaca
     5881 aggaaaaatg ctagacgatt actaaaaaga tacagaaatt tagaacgttt ggtcggacca
     5941 gtgaaaatag acttttccgt gatgactgtt accaaaaatt tgaaattcac aattgacagt
     6001 caaaacgaag aaatcattga agcgataagt actcgagatt cggttatcga agcattaaca
     6061 cggctaagta gaatccattt ccaggtactt tattatagtt attgttttcc taataagatg
     6121 tcaatgtatc aaataggaga aaaattagga tattccgata gaactatcga gagaatgaag
     6181 gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat ttcacgtaca
     6241 aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa gtattatatc
     6301 ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag acaccaagcg
     6361 cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt tcagcatatc
     6421 tatcagctag atgtagagta gggcaacatc gagaaccaaa agtgacagca tcttattcct
     6481 tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg atcgataaag
     6541 aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgttcgaggc tattcagcga
     6601 tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc tatgatcgtt
     6661 atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa taatactagc gaagaatcgg
     6721 ttaaagtaga atcaaacaga attatcatcc aatttgcttc atcgttagaa ctggttgctt
     6781 tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt ttccatttta
     6841 tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt gtcgaaaaat
     6901 taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     6961 agcttcgata ggcagcaacg gaaatattaa gaataaggat gtgaatttca actccttcta
     7021 aattgttctt attatctatc atccgttgct gtctattgtt tttttaatta ttcacacgat
     7081 aactaaaggt aagtgaagag aaatgattcc attaataatt tcaatttttg cgctttgtct
     7141 taatgtatat atgattggat ttaaaaatgg gcaaaataaa aaatagtagc agccaagaat
     7201 aattttatag tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc taggtcaatg
     7261 cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaaccg aggcatcggc
     7321 ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg taggttgcta
     7381 ttacataact ggttaggtta gattgagatt tgggattcgg tacaaatgaa tcgtcaaatg
     7441 tctcaagcac aggatcggaa acgtccctgc ctgtgcatta catattagat cactctttga
     7501 gtggtctttt tctttacata aaggaggcta cataatgaga aactactggt atgtatcgct
     7561 aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc gttctgtaca
     7621 aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat gtgagattga
     7681 tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca ttcaaaataa
     7741 tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct ccaatcagga
     7801 caaatcaagt tagacaaagt ttccgattta aagatattga ttgaagctga tttaatgcta
     7861 aaagatattg aaaattagaa aacaaaactc aacctaaaca gaatgtgagg tggtgtgtat
     7921 tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg gttagaatcc
     7981 aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga ttctcaggtt
     8041 agaaaatgga aatcgcaaga caaatggagc gctgaattga ttagtaacgt taccaatggc
     8101 aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc taaaggtaac
     8161 aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa aacaggtgag
     8221 tatgaaacaa tattttttga cacgttaacc gataatgaga aggacatcta ttctagtttg
     8281 aatgatgatc cttcttttgc tttgtccgaa gaaatacgac ttcttaaaat aagacaactt
     8341 cgaatgatga aaagaatcca acaagctgaa gctggactaa atgatgaaga agtcgaacga
     8401 ttgcagcagc taagaaagat taaaactccg attgaaaaaa atggtaaaaa gctagaaatc
     8461 aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa aattgatgat
     8521 attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa agccatcaag
     8581 caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc tcagactgat
     8641 aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga aatagaagaa
     8701 tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa tgaataatga
     8761 atttattcct tttgctgata ttggttctgc cattgattat tactatgata aaccagtagc
     8821 tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg ttttaaatga
     8881 tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa aaacagcatt
     8941 agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag taatagcaac
     9001 agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta aatggttaaa
     9061 tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata tggttggtga
     9121 ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaagta tgcaaggttt
     9181 tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg atccaattat
     9241 ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt gtggaaaccc
     9301 caataatatt gaaggtgtct tttatgattc ccataattca gaccgtgata aatacagagt
     9361 tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag aaatgattct
     9421 taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag aatttccgaa
     9481 aggtgcgttg gattcattta tcagtcttga aacagttgaa ttggctacag aaaaacaaat
     9541 tagtgattct ttagtcaata aaacaaaggt tgctcatatt ggtgtcgacg tagctcgata
     9601 tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg agtatgagaa
     9661 gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg ccaagaatct
     9721 aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca ctggtgtcgg
     9781 aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc cttttgaggt
     9841 gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt taggtactca
     9901 actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc ttaacggaga
     9961 acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa gtactcgcaa
    10021 attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata tgaaaaagcg
    10081 aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg agccaccaag
    10141 tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt attatcttta
    10201 gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg aatgtttgat
    10261 ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag aaaatatcta
    10321 gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac tgatattgta
    10381 aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca agcagtcaat
    10441 cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa aagaacactc
    10501 attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac agttccagaa
    10561 gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct tgattctgaa
    10621 actgcaactt ttcaaggagc ttgtggagta gctttccgat tgctagagcc tgtggaagaa
    10681 gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga aagatatgga
    10741 aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt ttttcaagaa
    10801 atgaaagtta tgacaagaaa taaaattctg acatatgctt gctatggtga tttgaatttt
    10861 gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg gacgattacc
    10921 ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc tgaggaaatt
    10981 ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca atttaagctt
    11041 gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga aatgatgaat
    11101 cagctaggga ttatcaactt aaaagcccct actgcaaaag ctgaatatgt cacaaaaaat
    11161 ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt ttacaccatt
    11221 tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg agaagctcgc
    11281 aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata ttttagagaa
    11341 ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga caaagtagaa
    11401 attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga tttaagttat
    11461 ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat tcagagccaa
    11521 attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga aagtggaagt
    11581 gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga tagaaacgaa
    11641 gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag aaaatcagac
    11701 acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag caaaactatt
    11761 atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt acagtacaag
    11821 taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa aaatgtatga
    11881 tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg caaaagtcaa
    11941 tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg cgatggatgc
    12001 agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa catattcttc
    12061 taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt taccagaaaa
    12121 tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa gaagactttg
    12181 gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa gtgttgctag
    12241 tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa tttgtgacat
    12301 gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc gaacggagta
    12361 tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta ttgaggaata
    12421 caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa aggcaagtaa
    12481 gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt tccatagtcg
    12541 ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg atgaagaata
    12601 tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg gaattaattt
    12661 gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt aatgtaaatg
    12721 ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct aatggcttga
    12781 gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact gttaattatc
    12841 aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta ttatctcatt
    12901 cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt attgttgtta
    12961 gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt ccgaaatgac
    13021 gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt aaaactcaaa
    13081 gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg aaacacaaat
    13141 cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag ttttcttttg
    13201 atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt cagtcacaat
    13261 ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta gataaaatta
    13321 aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct gagcaaattc
    13381 aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg acaagtgata
    13441 ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag ttaaaagagt
    13501 ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc actaagaatg
    13561 ctgttgaagg ttttacaagt gttttgaatg caattaatga gaaacatgca gatgcattaa
    13621 aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac caggttcagc
    13681 agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt caacaattta
    13741 actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt atttggatat
    13801 ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct atttacttgg
    13861 tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat atgatatcaa
    13921 caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg aatcaggtcg
    13981 aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat ctggtacatt
    14041 agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag cactacgtgg
    14101 tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag ctgatgaaaa
    14161 tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag caaataaggc
    14221 agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat ttaaattgat
    14281 gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata tgcaggagtt
    14341 aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg aaagagtaac
    14401 ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg ctctactaga
    14461 accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga aactacgtcc
    14521 attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac gtatcggtct
    14581 tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt taaatttaaa
    14641 gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca gtggtaaaaa
    14701 aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact aaaaattttg
    14761 ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat aacgaattta
    14821 tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt gatgttaatg
    14881 aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg agtctacaaa
    14941 agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa taattttgct
    15001 agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt taaaaagcgt
    15061 gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag tatcttcagt
    15121 tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta gtgagttact
    15181 tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg agttttttta
    15241 atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat tgaaagactg
    15301 acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa agtatatgat
    15361 aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt catagaagat
    15421 ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc taagattaaa
    15481 ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat tttttctcta
    15541 tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat aggaagtgag
    15601 aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga attttaaaaa
    15661 tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa tgaataaagt
    15721 cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc gaggatatcg
    15781 tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagaagtta taaacaatga
    15841 acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct ttgtcaaagg
    15901 gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc ctcgaagatt
    15961 taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat gaaatcattg
    16021 ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat cttgattcag
    16081 ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct ggaactgaaa
    16141 atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg attgataata
    16201 accctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt tttaatacga
    16261 ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa gcagatggtg
    16321 ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt gaaagataaa
    16381 tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact tggtttcctt
    16441 tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg ggaacagcag
    16501 taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat tcagtggttc
    16561 aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat ggatatactc
    16621 ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc ttgcgttctg
    16681 gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg aatgggaaat
    16741 accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa acatcaacga
    16801 ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt gtagatgtac
    16861 caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat aaagcaatga
    16921 tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt tcaggaggta
    16981 cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagacg aagaaggtaa
    17041 atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg tagaagatgc
    17101 tgcaggagtt atggaagaaa tgcaaacagc gactgataag caatcagtta aaaaagcttt
    17161 aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta ctggtgaaga
    17221 ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat tattgaagtc
    17281 tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag tgaggcgctc
    17341 aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa gacgcaatta
    17401 ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga gttaatgcgt
    17461 ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta tgataatttc
    17521 actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag atgatactag
    17581 atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac tcttttgatg
    17641 aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt tggaaaatat
    17701 ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc tttgatctaa
    17761 tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac gctctacgaa
    17821 atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga aagaatgggc
    17881 ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata agtcatgttc
    17941 aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa
    18001 atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct gttattcaat
    18061 tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca atgatcaacg
    18121 ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca atgggagatc
    18181 tgaaagaagg tttatctcaa ggtaagattt ctgttgaaga attccaaaat caattaatag
    18241 aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct aaagattcta
    18301 ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt ggtgttggtg
    18361 aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga tttaagggaa
    18421 ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct gcaaatattc
    18481 ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt ggttctgctt
    18541 tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg ttcatgtttc
    18601 aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat gccataattg
    18661 gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaatcca tttgttttaa
    18721 ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca accaatgaag
    18781 agcttagaaa caaggttata tctatttgga atgatgtgaa agattccgta attggtgtat
    18841 taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa atggcatcaa
    18901 atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt tctaacacct
    18961 ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag acttcaagaa
    19021 atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt tctgatactt
    19081 ggcaaagtat aaaagattca gccagagaaa aatggaatgg aatcaaaggt tctattatgt
    19141 cagttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt actttttatc
    19201 tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag attttaaaaa
    19261 atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga tgggaagaaa
    19321 caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata aatatttggg
    19381 aatctataaa gaatatattt gtaagttatt ttacaaatat ttattt
//
LOCUS       DASJTM010000052        19116 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJTM010000052 DASJTM010000000
VERSION     DASJTM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.052"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..705
                     /locus_tag="U4B29_001765"
     CDS             10..705
                     /locus_tag="U4B29_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2106712.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1147..1944)
                     /locus_tag="U4B29_001766"
     CDS             complement(1147..1944)
                     /locus_tag="U4B29_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN2106713.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1941..3125)
                     /locus_tag="U4B29_001767"
     CDS             complement(1941..3125)
                     /locus_tag="U4B29_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2106714.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3150..4397)
                     /locus_tag="U4B29_001768"
     CDS             complement(3150..4397)
                     /locus_tag="U4B29_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2106715.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4612..6357
                     /gene="dnaX"
                     /locus_tag="U4B29_001769"
     CDS             4612..6357
                     /gene="dnaX"
                     /locus_tag="U4B29_001769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN2106716.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6377..6691
                     /locus_tag="U4B29_001770"
     CDS             6377..6691
                     /locus_tag="U4B29_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN2106717.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6716..7312
                     /gene="recR"
                     /locus_tag="U4B29_001771"
     CDS             6716..7312
                     /gene="recR"
                     /locus_tag="U4B29_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN2106718.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7376..8020
                     /locus_tag="U4B29_001772"
     CDS             7376..8020
                     /locus_tag="U4B29_001772"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN2106719.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8035..8364
                     /locus_tag="U4B29_001773"
     CDS             8035..8364
                     /locus_tag="U4B29_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN2106720.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8378..9316
                     /gene="holB"
                     /locus_tag="U4B29_001774"
     CDS             8378..9316
                     /gene="holB"
                     /locus_tag="U4B29_001774"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN2106721.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9352..10176
                     /locus_tag="U4B29_001775"
     CDS             9352..10176
                     /locus_tag="U4B29_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN2106722.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10169..10516
                     /locus_tag="U4B29_001776"
     CDS             10169..10516
                     /locus_tag="U4B29_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN2106723.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10585..11457
                     /gene="rsmI"
                     /locus_tag="U4B29_001777"
     CDS             10585..11457
                     /gene="rsmI"
                     /locus_tag="U4B29_001777"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN2106724.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11566..12687
                     /gene="dinB"
                     /locus_tag="U4B29_001778"
     CDS             11566..12687
                     /gene="dinB"
                     /locus_tag="U4B29_001778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN2106725.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12741..13343)
                     /gene="nrdG"
                     /locus_tag="U4B29_001779"
     CDS             complement(12741..13343)
                     /gene="nrdG"
                     /locus_tag="U4B29_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN2106726.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13658..15847)
                     /gene="nrdD"
                     /locus_tag="U4B29_001780"
     CDS             complement(13658..15847)
                     /gene="nrdD"
                     /locus_tag="U4B29_001780"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN2106727.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16146..16940
                     /locus_tag="U4B29_001781"
     CDS             16146..16940
                     /locus_tag="U4B29_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106728.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16930..18972
                     /locus_tag="U4B29_001782"
     CDS             16930..18972
                     /locus_tag="U4B29_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106729.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5989 a         3477 c         3972 g         5678 t
ORIGIN      
        1 aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt ggatagtaga
       61 gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg atatcataaa
      121 tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga gggaacggta
      181 aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat caaagatctt
      241 gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc gtataatatt
      301 cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat
      361 atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta tcgtctgctt
      421 tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg
      481 caactttacg aattatatga tttattcggt gtgcggacag aagaagggat gctgatcgat
      541 ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc tagcagtgta
      601 aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga ccatcatatc
      661 gttattttga atatggactt gttagaagac aatattatct tttgatgaac aaagaggttg
      721 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
      781 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta ccaagaggtt
      841 gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
      901 ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt taccaagagg
      961 ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac agtctcttat
     1021 gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc gtttatttgc
     1081 aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac attcatttag
     1141 caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag aatgggtagt
     1201 acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg tcgtgacaaa
     1261 agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt ctcggaatgg
     1321 atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat aaaagacaag
     1381 actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca aaaccttata
     1441 gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa ctccgtcttc
     1501 cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg cagcatttgt
     1561 catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca aataatcgat
     1621 catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata gcaatactga
     1681 gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt ctcttggttc
     1741 ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt cattcagctt
     1801 ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag cctcatcatt
     1861 taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta gcaaaagaaa
     1921 gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag gatcgctagc
     1981 ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg caggacaccg
     2041 ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact tgagtcattg
     2101 cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc gtttgtgatt
     2161 gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc ttttaacagc
     2221 ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc atattcgata
     2281 tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat tgctttatct
     2341 aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga taaggcattt
     2401 tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg cttcgtttct
     2461 gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc ctgcatggca
     2521 ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa cttatcgatt
     2581 tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca agcgagaaat
     2641 ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg caagaattct
     2701 ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata agtcgcagca
     2761 gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag atctatcgct
     2821 gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc gtcaaccgct
     2881 ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt aaaatcttcg
     2941 gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt ttgcttttgc
     3001 tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca atgaaatttt
     3061 tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg tgtaagaaca
     3121 tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt aaacggttta
     3181 ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc attgcattca
     3241 ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc tccgcagttg
     3301 gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt atccgcattt
     3361 ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc ttggttattt
     3421 tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac acccataaag
     3481 ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc aatggaacct
     3541 tgcacgtagc cttcacgtac gctaggatca cctagcatcg ccccttcgtt acgcatattg
     3601 atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga aacaatctcc
     3661 atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt gcttaattcc
     3721 aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg attgattgct
     3781 gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt gatttccact
     3841 tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac gacatgaata
     3901 gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc aaagtagttc
     3961 aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat ataaaaaact
     4021 tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc agcaattgct
     4081 tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt gtaataacct
     4141 tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat catgtttgga
     4201 tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg attggagcca
     4261 cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc actcatattg
     4321 tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat acttgttgct
     4381 gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa acaattattg
     4441 tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg aattgaaatg
     4501 gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt ttttttacct
     4561 aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct tatggcatat
     4621 caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg acaaaaagct
     4681 gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc ctacttgttt
     4741 acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa agcaatcaac
     4801 tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg atcaattact
     4861 gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg agtcgaagaa
     4921 atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta caaagtgtac
     4981 attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt gaaaacattg
     5041 gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca caaaatacca
     5101 gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac ggcagatatt
     5161 gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc agctgcattg
     5221 aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat tcttgatcag
     5281 gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt gacaggtagc
     5341 ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga agtaccagaa
     5401 gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg ctttctagag
     5461 gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa cctagttgag
     5521 gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt ttcagcggat
     5581 cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg ttttaccaat
     5641 agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc gtctgcttct
     5701 gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct acaacaagag
     5761 attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat tgagaataca
     5821 aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta tcgtgtaccg
     5881 acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa ccaagtaaaa
     5941 gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat gcttaaagca
     6001 agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta tgagattgtt
     6061 tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa cttaagccga
     6121 atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg gccagaactt
     6181 cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt tgcttctgaa
     6241 gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga agaaacactt
     6301 gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga tgattaataa
     6361 aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga tgaaacaagt
     6421 acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa cagagtttac
     6481 aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc gcatgaaaga
     6541 tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc aggatcttgt
     6601 cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga aaaaattagg
     6661 gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa tgaatatgca
     6721 atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag gaatcggtca
     6781 gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag tagtaaatga
     6841 atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta tctgcggaaa
     6901 catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc gaagcgttat
     6961 cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag aatatcacgg
     7021 actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc cagaagacat
     7081 caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag tgattatcgc
     7141 aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc tgatcaaacc
     7201 cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg atatcgagta
     7261 tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat aaatttctaa
     7321 ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca acaatgtgaa
     7381 cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca tcttgaatga
     7441 actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa gagaaccagg
     7501 aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa atgatcggat
     7561 ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc tagtcgaaaa
     7621 ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg tcgacagttc
     7681 tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc ggttgaatga
     7741 gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag attcagacac
     7801 cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag attcggaagg
     7861 tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag agaatccgga
     7921 aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa caagttatca
     7981 tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac gtttatgaaa
     8041 attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga attgattgat
     8101 gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc tggaaacagt
     8161 acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat catcaaaaaa
     8221 acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat ttcgatggat
     8281 gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt cgtcttacct
     8341 gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa taaacttggc
     8401 tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg ggcgtatcgc
     8461 tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag gtatatggct
     8521 tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa aatgcaacaa
     8581 ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac cggaaggaca
     8641 aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca gcggttacga
     8701 atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca gtgcggcaaa
     8761 cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt tagaaacaga
     8821 tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc attttcaaga
     8881 actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat tggaaaaagc
     8941 aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct ctcaagatga
     9001 atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc aaaagcaaga
     9061 tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag aaaaaacaca
     9121 acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc gtcaagcagt
     9181 agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac ggatattgat
     9241 cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac agttcgtttt
     9301 gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga aatggtagaa
     9361 gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc aggagactct
     9421 gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca tattgctact
     9481 gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat ccgtccgatc
     9541 ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa agatgcttat
     9601 gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat gaaaatggtc
     9661 aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc ggacgggcga
     9721 attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg tattgagctt
     9781 cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc atgtgggcgt
     9841 ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa aatggcaaaa
     9901 gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg cttgatgtgc
     9961 tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc tgactttgga
    10021 aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct gcttagccgt
    10081 atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg ggatgaattg
    10141 aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg acggattgaa
    10201 tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga agcatgcttt
    10261 gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc tgcgggaaca
    10321 cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc aggaactttc
    10381 taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct gctacgattt
    10441 atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag tgatctatcg
    10501 cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac tgctccagtc
    10561 tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg aaatccggta
    10621 tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat gagctacaga
    10681 agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg taatacacaa
    10741 aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga acataattat
    10801 aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat tgctcaagtc
    10861 agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt agcctgcata
    10921 aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac ggctttgatt
    10981 gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg taagaaaaaa
    11041 gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat tttttatgag
    11101 tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg aaatcgccaa
    11161 gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg aagtacggaa
    11221 gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct gcttgtagaa
    11281 gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac gttaaaggaa
    11341 caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa agcagtagcg
    11401 ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt agactgagat
    11461 agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact tttcaaatct
    11521 cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat ggagctgcaa
    11581 tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga tgcttttttc
    11641 gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt aattgccaga
    11701 caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga agcaagaaaa
    11761 ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc gcaagccatc
    11821 tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg ggaagtattc
    11881 tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct tgatgtgacc
    11941 gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca agcagatatt
    12001 tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt tttggccaaa
    12061 ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca agacgctgtg
    12121 ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa aactgtacct
    12181 agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac agaaatgatg
    12241 ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag aggaatccat
    12301 gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca tacttatggg
    12361 cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc tgaaaaagta
    12421 gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa ggtcaggtac
    12481 acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta taaaaaagaa
    12541 gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga aaaaggaatc
    12601 cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa catcgtcctt
    12661 ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc ggaaatccgt
    12721 tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc ataggtttct
    12781 gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga tttttggacg
    12841 tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg tttgcttaat
    12901 tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt atcttctgaa
    12961 tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt tgtcgtaccg
    13021 aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa aaaaggttcg
    13081 ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat gatttgttct
    13141 tctaattcag gtgtgaatgg tttgccatat cgaaaatttt gtgcagcttt gttgaagcag
    13201 ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc ttcaccatcg
    13261 acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt tgctaaccat
    13321 tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca aagatatagc
    13381 tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta aatccattgt
    13441 tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc catcgaaaga
    13501 gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt tcaaaagctt
    13561 gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt tgtttagcta
    13621 ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt ttcacacgtg
    13681 aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt cctaaatagc
    13741 cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat tctggacatt
    13801 cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag caatgatcga
    13861 ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat acagcttcta
    13921 aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat ccacctgaac
    13981 aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg tttttacgca
    14041 catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg atgatgccga
    14101 atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga gttgaataga
    14161 cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt aattctttga
    14221 cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca ccgaagaaag
    14281 ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga cggtttttga
    14341 ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca tacatatata
    14401 aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag agaagcgcat
    14461 ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga tctccttttg
    14521 attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt cgtccgacat
    14581 ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag cccataggta
    14641 ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca ggatacattc
    14701 ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct tcagggttta
    14761 agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc cgcttttcac
    14821 tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt tcaaaccagt
    14881 tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa gtaaataagg
    14941 tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg gtttttgctt
    15001 ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct tttagatgtt
    15061 ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg gcagaacagc
    15121 caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct gtggcggtct
    15181 ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg ttcagcatgc
    15241 ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa tccaaatcat
    15301 ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac attttcagtc
    15361 cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa acattactgt
    15421 ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt tcaatcgaaa
    15481 agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa ttgatgtact
    15541 cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg ttttgaatct
    15601 catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc aagtctgcaa
    15661 tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc ttttctgctt
    15721 tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca tcgcgtttga
    15781 tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct tcataattta
    15841 ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca aaatcgggga
    15901 aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt ggatttctat
    15961 actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct ggtggatttg
    16021 ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt attcagtcca
    16081 agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta tgcaaataaa
    16141 tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa gtgaaacatc
    16201 taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat atctcattca
    16261 cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga aagacaacgc
    16321 tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg atccaaggaa
    16381 aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa aaactaggat
    16441 tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat atcctattgc
    16501 ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga catgtagctc
    16561 aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct gtcggacaga
    16621 gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt tttgcagatg
    16681 aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac ttgaagacca
    16741 tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat acagcaagct
    16801 actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt gtcagaagag
    16861 ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt ggcggaggag
    16921 gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc acgaggctat
    16981 cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt cagcgcaatg
    17041 acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga tggtggattg
    17101 agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag tgccaaccga
    17161 tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg tatgaataaa
    17221 ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt cgcctgtatt
    17281 tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt ggttcgaatg
    17341 atggatatgg atcttactag tccatttttt atctccattc catcagtagt agatacttta
    17401 tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa aatatggcgc
    17461 tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac aatgcgcttc
    17521 aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag cggatatttt
    17581 ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta tcttgataca
    17641 ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac ttatttgttt
    17701 ttctgttata catttcggtt atttttgatc atattgagta aatggaagat ttattactat
    17761 ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag ttggcgtaca
    17821 aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg cacggctagt
    17881 atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta tcagtttgat
    17941 gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa gataactaaa
    18001 gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct cgaagaacct
    18061 atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag aaaagttaat
    18121 ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct tgatcaaatg
    18181 caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc agataatcaa
    18241 gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat ggtatatcta
    18301 agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt ggattatcaa
    18361 gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg actgagcaat
    18421 gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt aaatggaagt
    18481 ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga agcacaattt
    18541 agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg tgcagttaat
    18601 cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt gattattgct
    18661 actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa agaaaattat
    18721 cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat cttcgagcaa
    18781 aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt atttgcaatc
    18841 aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct tgtgagtggc
    18901 ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata ttgtcggatt
    18961 atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt ttgaatacca
    19021 tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat tttttatatt
    19081 ttttaaaagt acgagaaatt ataaagaaaa aagcag
//
LOCUS       DASJTM010000053        18685 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJTM010000053 DASJTM010000000
VERSION     DASJTM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.053"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U4B29_001783"
     CDS             133..3660
                     /locus_tag="U4B29_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN2106730.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U4B29_001784"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U4B29_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN2106731.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U4B29_001785"
     CDS             7448..7789
                     /locus_tag="U4B29_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2106732.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U4B29_001786"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U4B29_001786"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN2106733.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U4B29_001787"
     CDS             9146..11566
                     /locus_tag="U4B29_001787"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN2106734.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4B29_001788"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4B29_001788"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN2106735.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U4B29_001789"
     CDS             13000..13737
                     /locus_tag="U4B29_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2106736.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U4B29_001790"
     CDS             13751..14566
                     /locus_tag="U4B29_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2106737.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U4B29_001791"
     CDS             14579..15226
                     /locus_tag="U4B29_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2106738.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U4B29_001792"
     CDS             15242..15898
                     /locus_tag="U4B29_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2106739.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U4B29_001793"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U4B29_001793"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN2106740.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U4B29_001794"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U4B29_001794"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN2106741.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..>18685
                     /locus_tag="U4B29_001795"
     CDS             18240..>18685
                     /locus_tag="U4B29_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2106742.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQ"
BASE COUNT         6269 a         3225 c         4017 g         5174 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaat
//
LOCUS       DASJTM010000054        18417 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJTM010000054 DASJTM010000000
VERSION     DASJTM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.054"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..516
                     /locus_tag="U4B29_001796"
     CDS             172..516
                     /locus_tag="U4B29_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN2106743.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="U4B29_001797"
                     /pseudo
     CDS             517..936
                     /locus_tag="U4B29_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="U4B29_001798"
     CDS             complement(1028..1378)
                     /locus_tag="U4B29_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106744.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="U4B29_001799"
     CDS             complement(1344..2675)
                     /locus_tag="U4B29_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN2106745.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="U4B29_001800"
     CDS             2931..3497
                     /locus_tag="U4B29_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2106746.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="U4B29_001801"
     tRNA            3642..3729
                     /locus_tag="U4B29_001801"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="U4B29_001802"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="U4B29_001802"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2106747.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="U4B29_001803"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="U4B29_001803"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN2106748.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="U4B29_001804"
     CDS             complement(7452..7898)
                     /locus_tag="U4B29_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2106749.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="U4B29_001805"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="U4B29_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2106750.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="U4B29_001806"
     CDS             8363..8881
                     /locus_tag="U4B29_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2106751.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="U4B29_001807"
     CDS             complement(8958..9914)
                     /locus_tag="U4B29_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2106752.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="U4B29_001808"
     CDS             10080..10886
                     /locus_tag="U4B29_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106753.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="U4B29_001809"
     CDS             10883..11872
                     /locus_tag="U4B29_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2106754.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="U4B29_001810"
     CDS             11869..12873
                     /locus_tag="U4B29_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2106755.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="U4B29_001811"
     CDS             13005..13547
                     /locus_tag="U4B29_001811"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN2106756.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="U4B29_001812"
     CDS             13567..14265
                     /locus_tag="U4B29_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2106757.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="U4B29_001813"
     CDS             14286..14498
                     /locus_tag="U4B29_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN2106758.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="U4B29_001814"
     CDS             14498..15460
                     /locus_tag="U4B29_001814"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN2106759.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="U4B29_001815"
     CDS             15478..15873
                     /locus_tag="U4B29_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2106760.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="U4B29_001816"
     CDS             16036..16401
                     /locus_tag="U4B29_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN2106761.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="U4B29_001817"
     CDS             16398..18209
                     /locus_tag="U4B29_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN2106762.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18417)
                     /locus_tag="U4B29_001818"
     CDS             complement(18271..18417)
                     /locus_tag="U4B29_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN2106763.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
BASE COUNT         5918 a         3116 c         3809 g         5574 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatat
//
LOCUS       DASJTM010000055        18082 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJTM010000055 DASJTM010000000
VERSION     DASJTM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.055"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            135..458
                     /locus_tag="U4B29_001819"
     CDS             135..458
                     /locus_tag="U4B29_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2106764.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            640..2010
                     /locus_tag="U4B29_001820"
     CDS             640..2010
                     /locus_tag="U4B29_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN2106765.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(2140..3441)
                     /locus_tag="U4B29_001821"
     CDS             complement(2140..3441)
                     /locus_tag="U4B29_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2106766.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(3795..5753)
                     /locus_tag="U4B29_001822"
     CDS             complement(3795..5753)
                     /locus_tag="U4B29_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN2106767.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(5930..6196)
                     /locus_tag="U4B29_001823"
     CDS             complement(5930..6196)
                     /locus_tag="U4B29_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2106768.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(6706..8832)
                     /gene="pnp"
                     /locus_tag="U4B29_001824"
     CDS             complement(6706..8832)
                     /gene="pnp"
                     /locus_tag="U4B29_001824"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN2106769.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(9020..9289)
                     /gene="rpsO"
                     /locus_tag="U4B29_001825"
     CDS             complement(9020..9289)
                     /gene="rpsO"
                     /locus_tag="U4B29_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN2106770.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            9540..10103
                     /locus_tag="U4B29_001826"
     CDS             9540..10103
                     /locus_tag="U4B29_001826"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2106771.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(10356..10877)
                     /locus_tag="U4B29_001827"
     CDS             complement(10356..10877)
                     /locus_tag="U4B29_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN2106772.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(10880..11722)
                     /locus_tag="U4B29_001828"
     CDS             complement(10880..11722)
                     /locus_tag="U4B29_001828"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN2106773.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(11715..12440)
                     /locus_tag="U4B29_001829"
     CDS             complement(11715..12440)
                     /locus_tag="U4B29_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN2106774.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            12616..12912
                     /locus_tag="U4B29_001830"
     CDS             12616..12912
                     /locus_tag="U4B29_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106775.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            13480..15072
                     /locus_tag="U4B29_001831"
     CDS             13480..15072
                     /locus_tag="U4B29_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2106776.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            15085..15825
                     /locus_tag="U4B29_001832"
     CDS             15085..15825
                     /locus_tag="U4B29_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2106777.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            15993..17342
                     /locus_tag="U4B29_001833"
     CDS             15993..17342
                     /locus_tag="U4B29_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106778.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(17470..18060)
                     /locus_tag="U4B29_001834"
     CDS             complement(17470..18060)
                     /locus_tag="U4B29_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2106779.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         5200 a         3680 c         3481 g         5721 t
ORIGIN      
        1 aaaagtattc aattattgtt gcatttttcc tgattcttta ttagaatgaa ccctcgttga
       61 agtaagtatt tctagtttat cctaggatta attataaaaa taaaacattc gttgattcga
      121 aggaggttaa acagatgttt gaaagttttg atagcagccg caaccgttac gcttcagttg
      181 gtgtcgtatc aagtttgcca gatgaattga ttgacagtat atggtttatt attgacctag
      241 atcttaaggg agtcattcca ttagaaaata tattaacctt tgatttgatc gaaaatcgtg
      301 gaaaagtgac gatgcatttt tctcaagcag gaagcaatgt ggaaatggat attgatttgc
      361 cgtttgccta ttcgaaccaa tttcctgctc aagtatacgc ctatgatgac ggtacgagag
      421 aaacgatcct tttaccaagt gagattagac aatattaagg tgatagataa acgtggatgt
      481 cccttatttt acagaccagg ccaagttgtt gattctttga ctcaattctt ggcttctttt
      541 tatggagttt cataaattat cattgatttc taattggtat tctcaggata aacgctatat
      601 actactaact aaattttaaa gatataagga gtttaaaaga tgcgcgattt aacaaaagga
      661 agccccgcta aactgatttt gatgttcact gttcctttac tgatcggaaa tgtttttcag
      721 cagttctaca atatgatcga tatgatcatc gtgggtcaga cgctaggtaa aaatgcgctg
      781 gcagcggttg gtgcaactgg aagcctgaca tttttgatca ttggctttgc ccaaggttta
      841 acagctggtt tagccattat taccgcacaa cgttacggtg caaaagatta tcggggtctg
      901 aagaaaagtt ttgctgctag tgttgtcatt agtcttgtcg tcactgtgat tttaactgtc
      961 ctaagtctcc tgtttattcg tccgatgctt caattgatgc aaactcctcc tgaaatactt
     1021 gatcaagcac aaacatttat ttcaattatt ttattaggga tctttgcatc agtcagtttc
     1081 aatttgcttt ctaatgtcat tcgcgcactt ggtgacagcc gcacaccact attctttctg
     1141 atcattgcgg tcatcatcaa cgtcgtactt gatttgatct ttattatcta ttttggcatg
     1201 ggtgtggaag gtgcagctat tgctacagta atcgcacaag tttcttccag cgtactgtgt
     1261 ctagtgtata tcaagaaaaa aatgcctttg ttacagttac gcaaaaaaga tttcaaattt
     1321 gaaaaagaag agtttgctgt acacctaaat gctgctttgc caatggcgtt tcaatcttcg
     1381 atcatcgctg ttggtgctat cgtgttacaa gcagcactaa atagtctagg gacagatgtc
     1441 gtagctgctc aagccgctgc tggtcgtatc gaccaatttg caaatcagcc gatgatgtca
     1501 tttggtattg caatggcaac tttcactgca caaaactatg gtgcgaaaga atatggccga
     1561 atcttgcaag gggtacgtca aactttactg atgagtgtcg gatttagtct cttagcaggt
     1621 gctatcgtca ttttcttcgg tcatttcttt gttcaattgt ttgttagccc atcagaaaca
     1681 cgtgtatttg aattagcaca aacctacttc aatatcaatg ggtctcttta ctggatctta
     1741 gcagtcctgt tcattttacg ctacacactt caagggcttg gacaaagcaa gatccctacg
     1801 atcgccggta tgatggaatt gcttatgcgt tcttttgcag ctatcgttct tacaggaatg
     1861 tggggatacc caggagcagc agcagcttct cctttagctt ggatcggttc tgttgccgtg
     1921 ttgctttact cttatatgcg ttcgatgaaa caattgaaac acatgggtga agaggaacat
     1981 ttcacattag aacatgcaga agcaagatag ccttgattaa aagaaatcgt tgcggctaaa
     2041 aatgaaaact tttattgaaa atatcttctt cgtttaccga aaacaggttt tttagataac
     2101 aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt tattctgttt ttctttccgt
     2161 ggactctact ttgaaataat cgatatgtgc atgctggtat tctaataccg tgaaacggaa
     2221 tgatgcaatc tcaatgattg ttcctgcttt tatttcttta tatcggctag aagcaaagcc
     2281 cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc tctacatgaa aataacgctg
     2341 gaaatcatct aatgttagtt tgccggagat gatataagag ccatctgact gttccgctat
     2401 caatgcttcg tccaaatctg tctcatctcg gatttccccg acgatttctt ctaaaatatc
     2461 ttcaatcgtc actaagccgc ttgtccctcc gtattcatct tttaagatag caatatgctt
     2521 accctttttt cgcatgatcg tcaataattt ttttataggg atcgtttcag ttgttacgat
     2581 cggctcttca atcaactgtt ctactgtcag ctggtcatct ttataagatt gcttgatcaa
     2641 atctggcaag gtgacataac ctaaaacatt gtcttttgat tctaagataa ccggatagcg
     2701 agtatgtcct tgttcaatcg aagcttttat cgcttctttg acggtttctg aagcttcgaa
     2761 tacttgcatg gacgtacggt ctacttgtat atcttttgcc aaggtatcat caaattcaaa
     2821 cacgttttcc aaataataat attcttcttt ggtcaactct cctttttcat aagaattcaa
     2881 tgcgactaat gtcagctctt cttgagacaa tgtctcttct ccttcagata cgagacgcac
     2941 acctaatagc ttccctagcc ctgcagctga atgattcaat acccaaataa aaggaaacat
     3001 tactttataa aaataatgaa gtggtttaac tacgaacaag acgaccttct ccgtattgac
     3061 gatactatag ctcttaggaa gcagttcgcc aataaccaca tcgatatacg taactaaaat
     3121 aaatcccagc gccacagaga ttgtactgct aatagccgaa ctaaaaggta gtttaccgat
     3181 cactggttct agtaaagccg cgacagatcc ttcaccgacc cacccgatga tcaaaccggt
     3241 tagcgtaatt cctaattgac tagctgataa atagttatct aaatggtgtg taacatgaag
     3301 agctagcttg gcgttcttta cattttcctt ttctagttgt tccaatcttg atcgtctgat
     3361 tttcactaaa gcaaattcac ttgctacaaa aaatgaagta attgcaatca tcaaaaataa
     3421 aataatgaaa ttgataacca tattctcccg cctttctctt tttctattgt accctttttc
     3481 tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa taaaaaagga gatgcggcat
     3541 ttttcagcca catctcctta tcacttcaaa atcattgcgt tacttgttgc taaacagctt
     3601 tttcatgacc tctgataaaa acggtattca aaagcttgca tctacggcat taagcccaaa
     3661 cagatgaaaa acatgagaag ctgtttttat ctgctcgttc ttactgcttg ttgcagtaca
     3721 gctttttcat gacctctgat aaacggtgtt caagtagctg acattcgaca ttagttcagg
     3781 tcaaagcgct actttcacaa ccgctctttc aaccatgttt tccgagtatt tccaatgata
     3841 agcggaacag tttcatgttt tacttcgctg tactctagcc caaaccatgt ttctggcgag
     3901 gtagcgattt ttttgattgc cagttttgcc tgcatgattt gacgttgcca ttttcctaga
     3961 gttgtcacat tggaaactac ttcatcaatc aggacaaatt ggaaatcgcc aacttctcgc
     4021 cctggtgtca gagagtatct ttgtggctgt tttggcaaac ggccttgttt catcaaatca
     4081 tggatgactt ggcgaacata tacgttgatt tcctgttgga ccctgaaacc taagaacaga
     4141 ttcacttgga caataaaatc agtgtccatc atatctactt gatattcttt cgtatatggc
     4201 tcatccgtca cttccacatg aacaaaccaa tagactcgtg ctcttttagg ccgtttatct
     4261 aaaatcgaat agatgaactg tcggccaact ttatcttcag ccatatcagg aaccaagaag
     4321 accacatttg tctgatagat tggtaaagaa gtatcatcac gtaattctct aagctgcggg
     4381 atataatctc tcaaagacac atcctcggtt gacgattcgc gaataatgtt cccttgttcc
     4441 caaataaaca tcacgactaa gatgatcaaa ccaatcagaa cggctacaaa accgcctcgg
     4501 aagaatttga caacacttga gataaagaaa acgacttcta tcgcaccgaa gaaaaaggta
     4561 actaaatatg caagccaacg agaatgccca ttttggataa gataaaagta aagcagtgct
     4621 gttgtcatca acatcgtcac agtgatcgat agaccgtaag cagcttccat atgatgagac
     4681 gttcggaatg tgacaaccac tagagaacag gcgatccata aaatcagatt gactgctggg
     4741 atatacattt gcccgatact gttcccagga tacatgatct ttaaccgagg cagcagtttc
     4801 aacttgattg cttctgaaac tagcgtaaac gaacctgtaa tcaatgcttg cgaagcaatg
     4861 actgcagcga ctgttgcaaa agcaacgccg atcacgatcc aactttgagg catcatttgg
     4921 aagaatggat tcaggttctt gatttgctgt gcttcagggt tttgataaac attcaacaac
     4981 cacgcagcct gcccaaaata attgataacc aaacaaattt tgacgtaagg ccagctcaga
     5041 cgaatatttc gtttgccgac atgtccaaga tcggaataaa gcgcctctgc acctgtcgta
     5101 gccaggaaaa tatttccaag aatgaacaag cctaatttgt tatctgcact aaaaagcagc
     5161 tggatagcat aataaggatt cagtgcacgg ataactgtcc agtcttgact gaagttcatt
     5221 aatcccatta cgcctaaaaa agtaaaccaa gcgaacatga ttggtccgaa tgcttttccg
     5281 acaatatctg taccaaagcg ttggaccatg aataaaacaa aaataataac taaagtgata
     5341 atgacgatca cgttctggtt attaccgaac cggtcaaaaa actgcggaat tcctcgcaag
     5401 ccttcaatcg cagtcgttac cgtcacagca ggggtcaata ccccatctgc tagtaaggca
     5461 gctcctccga tcattgcagg aatgatcaaa tatttcccac cttttcggac aagtgtgtag
     5521 agtgagaaaa ttccaccttc accatggtta tctgcattca atgcaatcac gacatatttg
     5581 attgttgtca gtagagtcaa tgtccaaaaa atcaatgaga ctgatccgat aacaaaatca
     5641 ggattgattc ctgacaatcc gccattatct tcaacaatcg atttcataac gtaaagcgga
     5701 cttgtcccga tgtctccgta gacgactccc atcgctacaa gcaggccagt cattgatatc
     5761 tttttggtat tgtgcgttgt tgccacgttt ttccctcatt tcttgttata tggatatcct
     5821 ttaggtaagc ttgaatgata gatgatattc ttatccatga cacccgcttt cctaaattgc
     5881 ttctaattat ccaagtaaca cactgaagtg tcaacttatt tgaataaaat tattctttct
     5941 ttggacgttc tctgatacgt cttgccacaa atgaataaag agcaaggcaa aaaattgcac
     6001 ccaataatgc tggtatcaaa taaaaaccgc cgagttccgg tcccagtggt cctagtaaaa
     6061 attcgccgag ccaactacct aaaaaaccga taatgatatt tccagatacg cctcccggga
     6121 cttcctttcc taaaattgcg ccagctatca aacctaatat accgccgata aataacgacc
     6181 ataaaaaatt cagcatctcc tttccctcct ttatccatat ttctcacttt tattttacgc
     6241 tttattcaga aggactccaa aaatatgcgc caagaaactc cagaaaaaaa gaagttgtaa
     6301 caaaagtgtt cagcttattt ccgtagaagc tgcttctgga acaccgtgaa tcagaggttg
     6361 tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
     6421 ttgccgtttt ttgagctaat atcgagggac agctgattga acatcgttta ttggagaggt
     6481 tgtaaaaaag ctgttctgca tcaagtaata agaacagcca aataaaaaca gctcctaatg
     6541 tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
     6601 aaaaaaggac tgtgacacgg cttttgtcac agtccccttt tttcgaataa ttattgtccc
     6661 cctgtcacct tcacgatcac aagtacatta ttcctatgaa ttaaattatt tttcttcatt
     6721 tttcgttgaa ccatcttcgt tcaacattgc ttttcttgac agatttacgc gtccttgttt
     6781 atcgatttca gttactttca caaggatctc atcacctagt ttaacaacat cttcgacttt
     6841 attgacacga tcgtttgcta attgggaaat atggacaagt ccatcttttc ctttgatcaa
     6901 gttgacgaat gcaccgaatt tttcgatacg gacaactttc cctagataaa cttcaccaac
     6961 tttgacttct ttggtcaaat cttcgatgat cttgatagct tttttgatca tttctgcatc
     7021 agctgaagca atacttactt ttccatcttg atcgatatcg attttcacac cagtttcatc
     7081 gatgattccg ttgatcgtat cgccgccttt accgataaca tctttgattt tagcaggttc
     7141 gatctggatc atttcgattt ttggtgcata ttggcttaat tcttcacgtg gagcagcaat
     7201 cgttgaagtc aattcttcta agatttccat acgtgcttgt tttgcttgag ttaatgcttc
     7261 agttaggatt tgttctgtga ttccttgaat cttgatatcc atctgtaaag cagtgatacc
     7321 atcttttgta ccggcaactt taaagtccat atcgccaaga tgatcttcca gcccttggat
     7381 atcagttagg atcgtatagt tttcaccatc agaaacaagt cccatcgcaa taccggcgac
     7441 tggcgctttg attggtacac ctgcatccat caatgctaat gtgccagcac agatacttgc
     7501 ttgggaagaa gaaccatttg attccaatac ttctgctact aaacggatcg tataaggaaa
     7561 ttcttcttct gaagggatta cttgtgctaa cgcacgttca cctaatgcgc catgtccgat
     7621 ttcccgacgc cctggagagc ctgcacgtcc tgtagaacca acagaaaatt gagggaaatt
     7681 gtagtggtgg atgaatcgtt tgctgacttc cacccctaaa ccatcaatga tttggtgctc
     7741 tcctagaggt gcgagtgtac aagctgataa agcttgtgtt tgtcctcgtg tgaacaaacc
     7801 tgatccatgt acacgtggca ataagctgac ctctgatgac aatggacgga tttcatccaa
     7861 tttacgcccg tcaggacgga tcttatcgat ggtgatcaat tcacgtacga catctttttc
     7921 aagatcttct gtgatttgtt taacttcttt caacaagtga tcttcatctt catgacctgc
     7981 aaatttctca gcataagctt cgcggatatc gatcttcacc tgctcgatat tatcttcgcg
     8041 agcaagtttt tcttcagtca ttactgctgc tttcattgta gcgtaagaag catcaaagat
     8101 ttcttttttc aatacttcat caacttgcag taaagtcact tccatttttt ctttccctac
     8161 tgcttgaacg atttcttctt ggaaagcaac taactctttg atagcgtcaa aaccaaacag
     8221 caatgcacca agcatgtctt cttctgaaac ttctttcgcg ccgctttcaa ccatattgat
     8281 ggcttgtttc gttccggcaa cagtcaattc gatatctgtt tgttctgctt gttctactgt
     8341 tggattcaag acgtactcgc catttacacg accaacttct acgcctgcga ttgggccgtc
     8401 aaatgggata tctgagattg atagtgctaa agaagaacct agcattgctg ccatagctgg
     8461 cgagcagtct gtttctacac tcatcacgat gttcgttact tggacttcat tacggaagcc
     8521 ttctgcaaac atcgggcgaa ttggacggtc gatcaaacgg gcagtcaatg ttgcttccgt
     8581 acttggacgg ccttctcgtt tgatgaaacc tccagggatt ttccctgctg catacatttt
     8641 ttcttcataa ttgatcgtta atgggaaaaa gtctgtatct tttgcttctt tagaggccac
     8701 agctgcactt aagactactg tatctccata acggactaaa acggctccgt ttgcttgttt
     8761 tgctagctga ccgacttcga tttgtaaggg acggccgccc catgtcgttt tgaatacttg
     8821 tttttctgac ataacttctc cttttccttc tcgtgaaagc aagacgctac taaacaaatg
     8881 aaaactgccg aaagccgtct tttgagcagc tttcatttgg ttagtaggag tccatctagc
     8941 ttgatcacgg aattttactt aattgatata cgagtaaaaa aagtgagatt catgtgaatc
     9001 ccactctttt tgactaaaat taacgacgca agcctaaacg tttgatcaat tcgcggtaac
     9061 gttgaacgtc agttttacgt aagtaagcta acaagttacg gcggtgacca acttttttca
     9121 ttaggccgcg gtatgaatga tggtcttttt tgtgcacgcg tgcatgttca ttcaagtggt
     9181 tgatttcttc agttaataca gcgatttgta cttctggaga accagtatcc ccttcatggc
     9241 gagcgtattc gttgatgatt tcattcttgc gttcttttga aattgccatt tctttcacct
     9301 ctttcgatta ttcccctgcg actgagtaaa acgttggtga ttcgcttaac caagtggcag
     9361 gtcatgtgcc tagagcacac agctatactt tacctgaaac cgggttgaaa atcaagtagt
     9421 taatcattaa gaaaaggtag ttttcaaaca atcttactac tacttttcca tttttgtggt
     9481 aaactataat gcgaaaatag acttgaaaca cagtctttta agaaaggggt acatattcca
     9541 tgattacaat ggatgatatc attcgtgaag gaaatccgac cttacgcgaa gttgcaaaag
     9601 aagtttctct tccattaagc gaagaagata tctctttagg aaaagagatg cttgagttct
     9661 taaaaaacag ccaagatcca atcaaagctg aagaactaca cttacgaggc ggtgttggct
     9721 tagctgctcc tcaattagat atttctaaaa gaatcattgc tgttcacgta ccaagtcctg
     9781 acccagaggc agatggacct tctctaagta ctgttatgta taatcctaaa attttgagcc
     9841 attctgttca agatgcttgt ttaggggaag gtgaaggctg tttatctgtt gatcgagaag
     9901 taccaggata tgttgttcgc catgccaaaa tcactgtgtc ttattatgat atgaatggtg
     9961 aaaaacacaa gattcgtttg aagaattatg aatcgatcgt cgttcaacat gaaatcgacc
    10021 atatcaacgg tgtgatgttc tatgaccaca tcaacgatca aaatccattt gcactaaaag
    10081 aaggcgtatt ggtgatcgag tagagaattg gcaggttcct tcgatactat gctacaagaa
    10141 agctttataa aaaaggggag tgctcaaaag ctgcacttcc cttttttatg tttctccact
    10201 aatcgaaatt tagatagaca tctagaaatc gcaattccaa aaaattctgt gtattggata
    10261 cgcatctttt cttatttttt cttatttctt ccatatgggt gtttatctca gagatgatag
    10321 aatagtttct aggataaaaa gtagcagcta cgatgttatc cttcgtagag gattcctgct
    10381 tcagctagtt tttgtttgat gtttttcagc atatgctttt ctgggtagcg gatcgtatgc
    10441 agatggatgc cgcctgttaa ttcggataac aaagtagcat tacttttttg ataacgtctc
    10501 ataaaggaat cgatatcttg tggcgttttg atgtgcaaag ttcctgctaa ctcaccgtaa
    10561 aggggatgtt ccacaattac atcaatgatt tcaccaccat tttcaacgat tagtctcagt
    10621 tcttcttctg tttgatcttt tccatgctgg acagcgattt tactgatgtg cccagtattg
    10681 gtttcttctg tttcgaggat ataacctctt gctgttgcca cgatactttt acctgcagct
    10741 cgtagtaaag caatatcgcc tacaatgatc tgacggctga ctccaaaagt tttcgcaaac
    10801 ttgctagcac tcactggttt ttctgtattt gccaaggttt gcatgatttg ttttcgccgt
    10861 gtctctcctt cgatcacact tattcctcct tcattttgtt ttggtattcg cgcgagtgtt
    10921 tcatcaaatc aacatacgta tctgttattg tttccttata tcccttattt ttcagcagac
    10981 taacaacttt atccaatact tgctgctctc tttttgcatc ataaactgct tggttttctt
    11041 cttttttcac ttgtcctatt ttagttaccg tatccattct tttttctaat aaagcaatca
    11101 actctcgatc gatcatatcg atcttatgac gtaatgtttc aagagactct tctgttagtt
    11161 cttcctctac tgtcttgatg atacgagcag gatttcctgc aatcactaca ttgtcgggaa
    11221 aagatttggt cacaaccgaa cctgcgccga ctactacgtt gtcccctaga gtgacacccg
    11281 gcgtgacgat aactcctccg cctaaccaaa cattattacc gattttaatg ggttttgcgt
    11341 actccagacc gctgttacgt ttcactggat gtagtggatg cgtcgccgtg taaagctgga
    11401 cattcggagc gaacatgcag ttatcaccga tttcaatcgt gctgacatct aaaaatgtac
    11461 aattgaaatt tgcataaaaa ttctccccaa caaatatatt atatccgtaa tcaaaattga
    11521 taactggctc catatagatt tttttccctg tacggccaaa agtctctttc aacaattggg
    11581 atcgcagttc cgctgattct gcttggttga tgatttggct ttgacttctt gcaaacatac
    11641 ggtcggcaac cagctcagga tcattggcaa aataaagctc gcctgcaatc attttttctt
    11701 tttcggtttt gaattcagtc ataatctgct cctaattctt gtattttttt gaaaacaacc
    11761 gctccgtctt cttgaataaa cgtataaggc tccgctaaat aaacaggcgt ttgattgatt
    11821 actaagattt tggctgacgg ctggcgaaac tgtaccagat cacaaaacgg atgaacttga
    11881 aaactcgttc caacaatgac gatcagctct gcttggctga cggcataagc cgcctgctcc
    11941 atgactccat cttgaaaccc ttcaccatat aacacgatgt ctgggcgtat ttgtcctccg
    12001 catgcttgat gtttgtcgct gtgaagatat tctttccagt ctaccgtttg ttgacatttt
    12061 cgacaatgac attgatacaa acttccatga aaattgacga gttgcgggct tttagccttt
    12121 gcgtgcaaac cgtcgatgtt ttgggaaaca acccaaacat ttttttcttt cgctagatgt
    12181 gccatttcca aatgaatgat attgggtttt gcttcaggat gataaatctt tttgacaaat
    12241 tgatagaatt tatccggttc attcaccatt gcttgatgac tcagcaagta ttccggctgc
    12301 tccatcccgt ggtagattcc tgacaaagat cgatagtctg gcactccaga aggtgtggat
    12361 acccctgcac cggtcaaaaa tgtaatttgc tgataagcta atatctcttt tgctgcttcg
    12421 tctactgtta attgtttcat gttttgtcac ctcactttcc attatagccc atactcgaac
    12481 aaatttattg ttgaaaagtc agaaaaatgg gaataaaaaa gtgttatggg catttgaaca
    12541 atttatcaca atgttcactg acaaaagaat gaaaacgtgt tacaatactg ctgtataaaa
    12601 gtgaggtgaa tcgatatgtt accattcttt atttattggg gcgttattct ggcttgtatc
    12661 gcctggttag cgttaagcgt ttatttctct gttttttatc ttgtacgcaa agaaaatgga
    12721 aatctttggg cttttgcttt attcaacgtg attgccgcaa ttgttttagc cattacatta
    12781 gccgtatatc gaacatgggg ctggggaatc acccaatact caagtttgat ttatctaata
    12841 ttagccattt atggcgtcgt tgttcttttg caagcgatcc taggacgtga gccaaagaaa
    12901 gcggcagctt aaatgatcga gatggaactg gacacaggaa attttctgtt ctagttcttt
    12961 ttttattgta ttacatacat ttttgtgtat aattatacta tcgtttgcaa tggatagatg
    13021 attcacattt tgattgtttt ttcggtaaaa gtaaagattt ctatatttat cagtattttt
    13081 tcaaaaaaca gcagttatag attgacaaag cctctcgagt atcgtaaact ttttttgtat
    13141 aaataactga atacgagatg cctcaatcaa tgaggtagag gtcgcgagaa tcattaaccc
    13201 tgtggagtga agcaaacatg gagaagcagg aatagggagg atcgccgaaa tgtgcacttt
    13261 tttgcttgga gtgtatgttg ggacgcaagt taagagcttg cggactgtct tagcagtgat
    13321 gctaagttgc gctatgtttt gtgagaggtt tatcacctta tggaatttta gcggcctttt
    13381 tatatctata aatcggtcgc tttttatttt ttcagataaa gctttaccgc atttcatcta
    13441 gaccgctcgt gtatcatata atcagaaagg gagaaaaaaa tgaaaaaaaa tatggtttca
    13501 gttgccgtac tatttgcttt tgtcttctca ctattcagtg tgggatctct ggcaaatgca
    13561 gatgaaaaaa caccgagcga tgagtttcgc gtaggtatgg aggctgggta tgcacccttc
    13621 aactggtctc aacaaacaga tgagaataat gccttgccaa ttcaaggcca aaatagttac
    13681 gccggcggtt atgatgttca aatcgctaaa aaagtagcag atggtttagg gaaaaaatta
    13741 gtcatcgtcc aaacgaaatg ggatggtctg gctcctgccc ttcaatcagg caagatcgac
    13801 gcgatcatcg ccggaatgag ccctactgct gaacgacgaa aagaaattga ttttaccgat
    13861 ccttattacg aatcacaatt agtagtagta gttcaaaaag atagcaaata tgcaaaagca
    13921 aaaagtttag cagatctttc tggagcaaag attactgctc agttaaatac attccactat
    13981 actgtgatcg atcagatacc agatgtgcag aaacaacaag caatggataa tttctcagca
    14041 atgagaacag ctttagcttc tggaatgatc gatggttatg tcagcgaacg tccggaaggt
    14101 gtaacggcaa ctagtgtgaa caaagacttg gaaatgcttg aattcagcgg tgcagatggc
    14161 ttccagacaa acccggaaga tgttcagatt tctgttggta tgcgtaaagg cgacccggat
    14221 atccaaaaag taaaccagat tctttctggc atctctaacg atgaacgaac caaaattatg
    14281 gaccaagcag tcaaagatca gcctgctgca actgatagtg aagaaaaacc tggcatcatt
    14341 tctgatttca ctacgatttg gaaacaatac ggcaatatgt tcttacgagg tgctggactt
    14401 accttgttca tcgcattgat cgggacagtt gtcggtacta cacttggatt actgattggt
    14461 gtcttccgtt cggtacctga atcaggaaat gcactgactc gcttcttcca aaaaatcgga
    14521 aatgcacttc tttctatcta tgtcgaagta ttccgcggaa caccgatgat ggtacaagca
    14581 atggtcatct tctacggtct ggcactcgct tttggcattt cattgaaccg aacgattgcc
    14641 gctttgttta ttgtctctct caatactgga gcttacatga cagagatcgt tcgtgggggg
    14701 atttttgcag tagatgaagg gcagttcgaa gctgcgcaag ccattggtat gacccatggc
    14761 caaacgatga gaaaagttgt tattccacaa gttttacgga atatcttgcc agcaacagga
    14821 aatgagtttg ttatcaatat caaagataca gcagttttaa gtgtaatcgg cgtagctgat
    14881 ttgttcttcc aaggaaactc tgcttccggt gcgaatttcc aattcttcca aacatttacg
    14941 atcgtcggga tcatctactt agtcatgacc tttacgatca ccagaatcct gcgggcggta
    15001 gaaaagaaaa tcgatggtcc ttcagcttat accaagcttg aagaaatcga caattcaaat
    15061 ttgcaggaat aaggaggaag aatcatgaat gaaaaaatta ttgaaatcaa acatctaaga
    15121 aaaagcttcg gtgaaaatga agtattgaaa gatatcagcg taacagtcaa taaaggtgaa
    15181 gttgtgacga tcatcggttc ttccggttct ggtaaatcta ctttcttgcg ctgtatcaat
    15241 ttactggaaa aaccaacagg cggcgagatc atttataatg gagaaaatgt attagctcct
    15301 aaatataatt tacctaaata tcgaacgaat ctaggtatgg ttttccaatc cttcaacctg
    15361 ttcaacaata tgaatgtctt ggaaaactgt atgtctggtc aaacaactgt cctgaaacga
    15421 gataaagaaa ctgccaaaaa agtagcaatc gaaaatctgg aaaaagttgg tatggatcgt
    15481 tatatcgaag ccaaaccagc acaattatct ggtggacaga aacagcgtgt cgcaattgct
    15541 cgggcattat caatgaatcc agatgtattg ctattcgacg aacctacttc ggcgttagac
    15601 cctgaaatgg ttggcgaagt gcttaatacg atcaaagact tagcacatac tggcttgaca
    15661 atgcttatcg tgacgcatga gatggaattt gcaaaagatg tatctgaccg tgtcattttt
    15721 atggataaag gtgtgattgc agaagaaggt acaccagaag atatctttgt ccatccgaaa
    15781 gaagaacgta caaaagaatt cctttcacgt attttaaatg cttaggatca atagctgatt
    15841 gggacaaaga gctgcggtgt ttaccgcagt tcttttttat gaattactca taaaaggtaa
    15901 atttctgaaa attttggaat cttagttgtg tttttcagaa aactctttta atatggaagt
    15961 aattaaactt ttttagttag gatgatttaa aaatgaatga agagcaagaa ctgcagcgtg
    16021 gactgaaaaa tagacatgtc caactgattt ctattggcgg agcgattgga acggggttgt
    16081 ttttaggttc cggaaagtct atccagctag ctggcccttc gatattgctc gcctatctta
    16141 ttactggatg catctgtttt ttcattatgc gtgcgttagg cgaacttctg ttatcaaaca
    16201 ccaataacca ttcttttctg gattttgttg cagaatatct tggcaaaaaa gcggctttca
    16261 tcactggctg gacgtattgg ttctgctggg tatcgattgc aatggccgat ttaacagcca
    16321 tcggaatgta tgtacgctac tgggcgcctg gtgttcctca gtggcttcct gaactgattg
    16381 cattaggtct tcttctttgc ttgaatatgg tggctgtctc attgtttggt gagttagagt
    16441 tttggtttgc attgatcaaa gttgtagcga tcattgcctt tatcctcgta ggagcttata
    16501 tgatcctcac ccactatacg acagatattg gcacagccag tatcacgaat ctatggtctc
    16561 atggcggatt ctttccaact ggtgcaaaag gatttatctt agctttccaa atggtaacct
    16621 ttgcttttgc tgggatagaa ttagttggat tagtcgctgg agaaacagaa aatcctgaaa
    16681 aagtgttgcc ggaagctatc aataatattc ctatcaggat catcttattt tacttaggat
    16741 ctctttttgt cattatggca atctatccat ggaacagctt gaatccagat aacagtccat
    16801 ttgtagaagt attttcggaa attgggatca cgattgctgc ttccttgatc aatttagtcg
    16861 tattaagtgc agcagcttct gcctgcaaca gtgcaatata tagtacagga cgtatgcttc
    16921 gttctttagc gcaagaaggc agtgccccta aaaaattcaa aaagttaaca acacatcatg
    16981 tgccagggaa tgctttgaca ttttctacta tcgtcatttt catttctgtt atcttgaatt
    17041 acgtgatgcc tagtgaagta ttcacactag taagcagtat tgccactact tgtttcttgt
    17101 ttatctggag tatgcttgtc tacacgcaca tgaaatatcg taaaagtatc ctcggcaaaa
    17161 aagctcattc atttaaaatg ccgttgtacc catttagtaa ttatctggta tttctttaca
    17221 tggcctttgt gtgtgtcgtc ttgttcctag gaaaagatac gagaatcgct ttattactaa
    17281 cacctgtgtg gttcttgctt ctattactaa tctatcatat gaaatatgaa cgtaagaagt
    17341 aagaaagtag ttgtgacaga agtggtcagc ttcgagaaat aagaaggaat ttacgaaaat
    17401 tgcttcttaa atttttgtga aatttcggct tatttcttgg agttaaaagc ttctgtccca
    17461 gcctcttttc tatttccccg gaaacaaccg ctgataatag gcatctgctt ttttatagga
    17521 aaaaatccaa ataccagata caaaaaaacg tccgaataac ccgatcaacg gtacaaaaaa
    17581 gacaccggca atcaagcata agttaatcat catggagata agacttcgac ggtcctttga
    17641 caaaactctc acgacatggg aatctggttt atttgctttc atgatttgcc tcatcaagat
    17701 accaaaagaa aaactccaca tcgtgtatac taagacataa gccatctcag gccaaaaagc
    17761 ttcagggtta cgcccgaccc aactagtaac agaaggacat aaagtcagcc aaaagagcaa
    17821 aaagatattc gaccagacca ctcggtaatt aattcgttct gccatctgaa atatacgatg
    17881 atggttcacc cagatcaccg caacaaatat aaagctgatg gtataagcta ataatgtatt
    17941 tcggatgacc cataaagaat gaaagctaga tgtattaggg atcttgatct ctaatacaag
    18001 aactctcaaa acaatggcta ccactccatc agtaaatgct tctaatctcg actttgtcac
    18061 gatgattcct cctcagatga tt
//
LOCUS       DASJTM010000056        17309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJTM010000056 DASJTM010000000
VERSION     DASJTM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.056"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(68..613)
                     /locus_tag="U4B29_001835"
     CDS             complement(68..613)
                     /locus_tag="U4B29_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2106780.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(625..1317)
                     /locus_tag="U4B29_001836"
     CDS             complement(625..1317)
                     /locus_tag="U4B29_001836"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN2106781.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1336..1620)
                     /locus_tag="U4B29_001837"
     CDS             complement(1336..1620)
                     /locus_tag="U4B29_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106782.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1633..2196)
                     /locus_tag="U4B29_001838"
     CDS             complement(1633..2196)
                     /locus_tag="U4B29_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2106783.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2424..3086
                     /locus_tag="U4B29_001839"
     CDS             2424..3086
                     /locus_tag="U4B29_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN2106784.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3210..4406
                     /locus_tag="U4B29_001840"
     CDS             3210..4406
                     /locus_tag="U4B29_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN2106785.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4507..5883)
                     /locus_tag="U4B29_001841"
     CDS             complement(4507..5883)
                     /locus_tag="U4B29_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106786.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5920..6786)
                     /locus_tag="U4B29_001842"
     CDS             complement(5920..6786)
                     /locus_tag="U4B29_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN2106787.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6875..7900)
                     /locus_tag="U4B29_001843"
     CDS             complement(6875..7900)
                     /locus_tag="U4B29_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2106788.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7918..8592)
                     /locus_tag="U4B29_001844"
     CDS             complement(7918..8592)
                     /locus_tag="U4B29_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2106789.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8776..9669)
                     /locus_tag="U4B29_001845"
     CDS             complement(8776..9669)
                     /locus_tag="U4B29_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2106790.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9743..11440)
                     /locus_tag="U4B29_001846"
     CDS             complement(9743..11440)
                     /locus_tag="U4B29_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2106791.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11433..12746)
                     /locus_tag="U4B29_001847"
     CDS             complement(11433..12746)
                     /locus_tag="U4B29_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2106792.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12746..16783)
                     /locus_tag="U4B29_001848"
     CDS             complement(12746..16783)
                     /locus_tag="U4B29_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2106793.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16796..17254)
                     /locus_tag="U4B29_001849"
     CDS             complement(16796..17254)
                     /locus_tag="U4B29_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2106794.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4840 a         3893 c         3015 g         5561 t
ORIGIN      
        1 agtcgtttgt tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt
       61 tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat
      121 gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat
      181 tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa
      241 tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat
      301 aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt
      361 ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc
      421 ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat
      481 ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg
      541 ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga
      601 tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc
      661 tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc
      721 tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg
      781 tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc
      841 tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc
      901 gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc
      961 ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc
     1021 atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat
     1081 tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact
     1141 tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt
     1201 acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag
     1261 gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt
     1321 tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat
     1381 aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct
     1441 gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat
     1501 ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct
     1561 tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat
     1621 aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt
     1681 ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg
     1741 atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc
     1801 ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt
     1861 aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt
     1921 catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac
     1981 tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc
     2041 aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg
     2101 caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact
     2161 gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat
     2221 cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata
     2281 gataaacctt agattttctc taaatataga aaatccttct ttagtccgat actctttcta
     2341 gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg
     2401 ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg
     2461 tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga
     2521 taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag
     2581 aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag
     2641 cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat
     2701 tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag
     2761 tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag
     2821 caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat aagtataatg
     2881 agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc
     2941 agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg
     3001 atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata
     3061 aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc
     3121 agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg
     3181 agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg
     3241 ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag
     3301 atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg
     3361 ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa
     3421 aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat
     3481 taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag
     3541 gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg
     3601 ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag
     3661 tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata
     3721 aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca
     3781 aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac
     3841 ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg
     3901 atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg
     3961 atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa
     4021 acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt
     4081 ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc
     4141 aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa
     4201 tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc
     4261 aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag
     4321 gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac
     4381 aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca
     4441 acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat
     4501 tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag
     4561 tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa
     4621 tacaatacaa gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc
     4681 tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat
     4741 caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc
     4801 ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt
     4861 ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa
     4921 acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac
     4981 agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc
     5041 attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta
     5101 aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt
     5161 tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag
     5221 agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg
     5281 tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac
     5341 catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa
     5401 tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc
     5461 agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga
     5521 ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt
     5581 cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac
     5641 tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc
     5701 taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac
     5761 cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt
     5821 tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc
     5881 cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc
     5941 taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt
     6001 tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat
     6061 ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat
     6121 gtttcctaag ccccataata atccacagat catgtttttc cacacaaact gatcgacttt
     6181 aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat
     6241 aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat
     6301 cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc
     6361 tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa
     6421 agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc
     6481 cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc
     6541 catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa
     6601 aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa
     6661 agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat
     6721 tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat
     6781 ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac
     6841 aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta
     6901 agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat
     6961 ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg
     7021 ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt
     7081 ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt
     7141 ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt
     7201 ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga
     7261 tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa
     7321 tgattccttt tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg
     7381 cataatacaa aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg
     7441 tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa
     7501 gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac
     7561 tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta
     7621 ccagtttttc ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt
     7681 gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa
     7741 aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga
     7801 tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga
     7861 tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca
     7921 gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc
     7981 gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc
     8041 gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat
     8101 caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc
     8161 catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa
     8221 ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat
     8281 ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat
     8341 attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg
     8401 acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc
     8461 ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg
     8521 ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat
     8581 gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc
     8641 cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg
     8701 gaaatatgcg atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc
     8761 ttaataattg gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg
     8821 ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa
     8881 tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg
     8941 aactcgtctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat
     9001 aagtgtgact agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac
     9061 aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg
     9121 gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg
     9181 ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac
     9241 tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt
     9301 gattttcgga atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt
     9361 ttggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac
     9421 tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt
     9481 ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata
     9541 attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat
     9601 cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt
     9661 ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat
     9721 tgctgcagct tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc
     9781 tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac
     9841 cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta
     9901 ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg
     9961 ctgcctcacc tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg
    10021 ttccataatc taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg
    10081 cttgtccgct tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg
    10141 actccccttt ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg
    10201 catctttaag caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg
    10261 tgactttctc tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt
    10321 caataaccgc tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat
    10381 tatacgtgat cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt
    10441 tggctactcc attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta
    10501 ctatataatc ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat
    10561 cataataagt tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc
    10621 ttccgttttc aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag
    10681 ccgtttttcc tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa
    10741 taatcgttac ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga
    10801 tttcgttttt cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat
    10861 cccacacgcc atccaccata tcaggcatca tcactggcat cataacaacc attggagacg
    10921 cgattctgga aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag
    10981 aagaaggtgc ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg
    11041 ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt
    11101 tttgaatcca ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag
    11161 gattatctcg aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc
    11221 ctggaatatt ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg
    11281 gaacagatga gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga
    11341 cttcttcggc tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta
    11401 acagtacact cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt
    11461 tttattaata aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt
    11521 gcttcattcg tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca
    11581 ctcggctctt tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt
    11641 gcggattcat aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg
    11701 ttaactacta catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc
    11761 tcgattctct tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa
    11821 taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa
    11881 atgaatttgc ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga
    11941 tttttcaagt cattagcagg agccatatct aaataatatt tccccccttc tatcatctga
    12001 tatagagcaa aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt
    12061 ccatacttga agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc
    12121 tcttgcagtt cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg
    12181 atagctgtct gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa
    12241 atcaaataaa ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg
    12301 tctcctaatc cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc
    12361 ctttcttctg tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg
    12421 aattctttag gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg
    12481 taaatggcaa atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc
    12541 cctgcttcct cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa
    12601 tcgatatctc gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct
    12661 tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga
    12721 agcatcaaac acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa
    12781 acaaaccata ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata
    12841 atcgtccgga tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct
    12901 gtaatctgat tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg
    12961 tctctgcttc ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc
    13021 ctggaactac ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa
    13081 agtaacctgg aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc
    13141 cttccaacaa gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc
    13201 cctcgatgat ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt
    13261 caagacgtcg gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt
    13321 catcttctaa ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag
    13381 cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa
    13441 actggaacac ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat
    13501 attcaccaat agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc
    13561 ctacggcacg agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta
    13621 acggattacc agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat
    13681 cgaaacctgc accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc
    13741 gataatcaaa cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg
    13801 tatacgtgag atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag
    13861 cattatcgta aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg
    13921 ggttctggct ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat
    13981 aaacaaatga atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat
    14041 atttcccgtc ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt
    14101 tcacaccaga aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt
    14161 cataaagttc aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga
    14221 tattcatgtg gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat
    14281 actctgtgcc ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat
    14341 aataggaatt cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat
    14401 ctacaagctc gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga
    14461 attgatacgt ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa
    14521 atatgtcgct gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg
    14581 ctattccgtc cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat
    14641 tttggtcata taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc
    14701 tgacttggaa attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc
    14761 gtccagtagt gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca
    14821 ctgtgcaaaa atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga
    14881 ccatttcccc ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga
    14941 catacgttgt cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc
    15001 atatttctgg ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa
    15061 tatctgccga tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg
    15121 tggcttgctg gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt
    15181 cattcttgtt gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa
    15241 tatttttcaa tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat
    15301 cgattcctaa accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc
    15361 aatgatgata agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat
    15421 tcgtattgtt attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt
    15481 ttacctctcc ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg
    15541 cataggtaac tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat
    15601 ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc
    15661 aagctccatt agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg
    15721 tcccatctcc cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt
    15781 tatacaatgt ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg
    15841 tacgatttaa tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa
    15901 agatgtccga actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca
    15961 ccataccgat tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta
    16021 ctgcctcaat caacgcagca tttttagttt gactattggt cccagcggtt aattcactca
    16081 tactcgctga cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc
    16141 ccccgatgac atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag
    16201 atgctgtgct gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat
    16261 ccaagtaact gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa
    16321 cgtttctatc ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc
    16381 agttcgctcc cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg
    16441 agcctttgtc tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat
    16501 agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat
    16561 agatatattc ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga
    16621 ataactggcg ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct
    16681 tttccatctc attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat
    16741 tttcttgtaa ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc
    16801 cttcgttcac atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt
    16861 ttgaataaac ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt
    16921 ctccatcttc atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc
    16981 cggcggctgc cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt
    17041 caaaagccag atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg
    17101 catagtcctc atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat
    17161 ccagggtcgt catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta
    17221 ctaactgcaa aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt
    17281 tgtcttcttt cacaacaatc aacaaaaca
//
LOCUS       DASJTM010000057        16848 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJTM010000057 DASJTM010000000
VERSION     DASJTM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.057"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1284
                     /locus_tag="U4B29_001850"
     CDS             <1..1284
                     /locus_tag="U4B29_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106795.1"
                     /translation="LGGAIGAGLFKGSGEAIGIAGPSVLIAFLIGGLILYIVMKGLGK
                     IVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIINIIAEAVAAASFLQLWFPHI
                     PAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVIILLIIFATYLVGQQMLGTG
                     VFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELIAITVSEADDPKRAIPKAIK
                     GVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFEKMNIPFAADIVNFVIVLAL
                     FSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQRAVLFCTSVLYLGVALSYF
                     VGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLWDKGISFFALAVLGLIFLGI
                     LFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1502..1945
                     /locus_tag="U4B29_001851"
     CDS             1502..1945
                     /locus_tag="U4B29_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN2106796.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2449..3867)
                     /gene="nhaC"
                     /locus_tag="U4B29_001852"
     CDS             complement(2449..3867)
                     /gene="nhaC"
                     /locus_tag="U4B29_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2106797.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4270..5493)
                     /locus_tag="U4B29_001853"
     CDS             complement(4270..5493)
                     /locus_tag="U4B29_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN2106798.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5524..6363)
                     /locus_tag="U4B29_001854"
     CDS             complement(5524..6363)
                     /locus_tag="U4B29_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2106799.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6380..7060)
                     /locus_tag="U4B29_001855"
     CDS             complement(6380..7060)
                     /locus_tag="U4B29_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106800.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLPANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7062..8135)
                     /locus_tag="U4B29_001856"
     CDS             complement(7062..8135)
                     /locus_tag="U4B29_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2106801.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8191..8429)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8472..8705)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8782..9453)
                     /locus_tag="U4B29_001857"
     CDS             complement(8782..9453)
                     /locus_tag="U4B29_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2106802.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9540..12218)
                     /gene="mgtA"
                     /locus_tag="U4B29_001858"
     CDS             complement(9540..12218)
                     /gene="mgtA"
                     /locus_tag="U4B29_001858"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN2106803.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12877..13314
                     /gene="ndk"
                     /locus_tag="U4B29_001859"
     CDS             12877..13314
                     /gene="ndk"
                     /locus_tag="U4B29_001859"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN2106804.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13336..14202)
                     /locus_tag="U4B29_001860"
     CDS             complement(13336..14202)
                     /locus_tag="U4B29_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2106805.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14362..16455)
                     /locus_tag="U4B29_001861"
     CDS             complement(14362..16455)
                     /locus_tag="U4B29_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2106806.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4853 a         3503 c         2972 g         5520 t
ORIGIN      
        1 ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc
       61 ggcccttctg tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa
      121 ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata
      181 cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg
      241 attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg
      301 cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt
      361 tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc
      421 attatcttac tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc
      481 gttttcccta cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag
      541 ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct
      601 atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc
      661 atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct
      721 tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat
      781 atcccatttg cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc
      841 aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt
      901 tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt
      961 tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt
     1021 ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg
     1081 gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt
     1141 gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta
     1201 actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat
     1261 gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata
     1321 gatggagcta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa
     1381 taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg
     1441 attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta
     1501 aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg
     1561 tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt
     1621 catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat
     1681 gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg
     1741 ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat
     1801 gattcctcat cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga
     1861 agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa
     1921 aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc
     1981 agaagttatc agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg
     2041 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct
     2101 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag
     2161 aggttgtaaa atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct
     2221 catattttgc cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga
     2281 gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc
     2341 cacatttttg tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc
     2401 gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa
     2461 ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc
     2521 tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa
     2581 gtaagtgagt gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg
     2641 cactaagtag ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc
     2701 aaatcctgct ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa
     2761 ttgttcacca ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt
     2821 tggtgtgttc gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc
     2881 gatcaacaat ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact
     2941 ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa
     3001 ccctgattcg atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat
     3061 cattcctact gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca
     3121 tgcacacagt aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc
     3181 ttctagaaca tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc
     3241 taaaatcatg aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg
     3301 cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga
     3361 taatggagac atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc
     3421 tggatttaag cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac
     3481 agtaaaggca ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac
     3541 aaaccactgg acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc
     3601 ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc
     3661 tggtttgatg ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac
     3721 tgcatacaac atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa
     3781 tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa
     3841 aaaacttggt gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt
     3901 ctaaaaaagt taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata
     3961 aaaataaaaa gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa
     4021 aaacagatct gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt
     4081 cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc
     4141 gctactgcgt gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg
     4201 aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttca cacacaagat
     4261 tattttttac tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc
     4321 ttgcttgatt ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag
     4381 tagttctgct gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa
     4441 ttcttctgtg tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg
     4501 tatgacttgg ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc
     4561 tatttcttca tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga
     4621 aggagtaacg atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc
     4681 cgtacttatc gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt
     4741 ttgccgattt cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc
     4801 agctactcct ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg
     4861 tagagcgttg attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc
     4921 aggtgctgct gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc
     4981 taaagattct gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac
     5041 atcctcaaaa aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc
     5101 ttcccctggg gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac
     5161 tatgctggcg gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat
     5221 acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat
     5281 ataacgtgca tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc
     5341 tttttctaat tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg
     5401 atgttcgtgg atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc
     5461 cttcaattgg tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt
     5521 ttcttatttt ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc
     5581 gattactttg actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt
     5641 gttttcatct tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac
     5701 tggttctaag aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct
     5761 attgatcact gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc
     5821 agaaatgtct agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt
     5881 tgccttatcc actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt
     5941 tgtaacatca ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa
     6001 agtgttagaa taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt
     6061 tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat
     6121 cgatccatct gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa
     6181 attaatgttc tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc
     6241 aataatccct agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct
     6301 acaccctgcc aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt
     6361 cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt
     6421 gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga
     6481 atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag
     6541 ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag
     6601 gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt
     6661 ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa
     6721 aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc
     6781 caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac
     6841 gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct ggtaatccat
     6901 tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg
     6961 ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca
     7021 aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc
     7081 actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg
     7141 atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa
     7201 ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt cgctgttact
     7261 tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt
     7321 aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt
     7381 tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca
     7441 ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc
     7501 gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt
     7561 aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga
     7621 tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt
     7681 gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt
     7741 tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat
     7801 tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga
     7861 ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag
     7921 agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa
     7981 ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca
     8041 tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt
     8101 ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa
     8161 gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc
     8221 tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga
     8281 tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat
     8341 tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc
     8401 tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata
     8461 aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc
     8521 ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg
     8581 aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac
     8641 tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa
     8701 atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa
     8761 ttcaaattat tttttctata tttatttttc tgttttttta tttaacattt caataacagt
     8821 tcgaagatct tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt
     8881 tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt
     8941 tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc
     9001 caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt
     9061 aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt
     9121 aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc
     9181 ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc
     9241 acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc
     9301 aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat
     9361 atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa
     9421 caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat
     9481 acacatagtt tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt
     9541 tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga
     9601 taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct
     9661 aattcagcac caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg
     9721 ataacaaaca tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt
     9781 gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat
     9841 gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca
     9901 ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga
     9961 ccaaagtaaa ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa
    10021 tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc
    10081 aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc
    10141 acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc
    10201 ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa
    10261 aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata
    10321 tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga
    10381 ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat
    10441 acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca
    10501 ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat
    10561 tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct
    10621 tgtttcgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct
    10681 gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca
    10741 atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt
    10801 tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt
    10861 tcttcaatcg caccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca
    10921 ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta
    10981 tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc
    11041 cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct
    11101 tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc
    11161 gtacataaca catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt
    11221 ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc
    11281 atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc
    11341 ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc
    11401 agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat
    11461 agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc
    11521 gcacttccac tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt
    11581 tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat
    11641 gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata
    11701 tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg
    11761 tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc
    11821 gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg
    11881 aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg
    11941 atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc
    12001 aatatttcta caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt
    12061 tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca
    12121 aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt
    12181 ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct
    12241 ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat
    12301 agccctggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat
    12361 ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc
    12421 caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct
    12481 ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc
    12541 atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat
    12601 gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc
    12661 agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta
    12721 atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta
    12781 aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg
    12841 ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc
    12901 aagccagacg gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag
    12961 ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat
    13021 tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt
    13081 cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt
    13141 ggagcaacaa aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg
    13201 gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc
    13261 cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa
    13321 ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga
    13381 cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt
    13441 cgtcttttct tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt
    13501 ttcatagatc actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac
    13561 actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa
    13621 agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat
    13681 tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat
    13741 agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca
    13801 ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag
    13861 attcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat
    13921 ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt
    13981 taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt
    14041 cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc
    14101 aatcccgact agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt
    14161 cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa
    14221 tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa
    14281 taaaaaaatg tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact
    14341 actgaaggtt ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt
    14401 tactggatat aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg
    14461 ttcgcctttt tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt
    14521 tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt
    14581 cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc
    14641 atcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc
    14701 tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc
    14761 atgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa
    14821 tagatgtagt aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc
    14881 tttagtataa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa
    14941 ttcatcccat gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt
    15001 cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt
    15061 tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata
    15121 attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt
    15181 ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg
    15241 ctcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt
    15301 ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata
    15361 tacatgatct ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc
    15421 ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt
    15481 gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact
    15541 tgtctttccc tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg
    15601 gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa
    15661 ctgctggaaa ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt
    15721 ttcgggattt ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt
    15781 actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt
    15841 atacgcatcg attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa
    15901 ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca
    15961 ggtaacagcg atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac
    16021 cttcaaccaa accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg
    16081 gggcttcatc aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa
    16141 tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa
    16201 caaaagcgta tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt
    16261 aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat
    16321 aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt
    16381 cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt
    16441 tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg
    16501 aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat
    16561 tattccgtcc acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag
    16621 agataaaagt cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta
    16681 ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca
    16741 ggacagcttt ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca
    16801 agtcacaata tttataaaac atgagaagct gttttttgaa attgttcc
//
LOCUS       DASJTM010000058        15878 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJTM010000058 DASJTM010000000
VERSION     DASJTM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.058"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4B29_001862"
     CDS             226..894
                     /locus_tag="U4B29_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN2106807.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="U4B29_001863"
     CDS             complement(1094..1324)
                     /locus_tag="U4B29_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106808.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="U4B29_001864"
     CDS             complement(1451..2833)
                     /locus_tag="U4B29_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2106809.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="U4B29_001865"
     CDS             complement(2989..4176)
                     /locus_tag="U4B29_001865"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN2106810.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="U4B29_001866"
     CDS             complement(4183..5214)
                     /locus_tag="U4B29_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2106811.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="U4B29_001867"
     CDS             complement(5230..6684)
                     /locus_tag="U4B29_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2106812.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="U4B29_001868"
     CDS             6859..7764
                     /locus_tag="U4B29_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2106813.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="U4B29_001869"
     CDS             7855..8532
                     /locus_tag="U4B29_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2106814.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="U4B29_001870"
     CDS             8529..8699
                     /locus_tag="U4B29_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106815.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="U4B29_001871"
     CDS             8813..9709
                     /locus_tag="U4B29_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2106816.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="U4B29_001872"
     CDS             complement(9764..11134)
                     /locus_tag="U4B29_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2106817.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="U4B29_001873"
     CDS             11372..12118
                     /locus_tag="U4B29_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2106818.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="U4B29_001874"
     CDS             12115..12954
                     /locus_tag="U4B29_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2106819.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="U4B29_001875"
     CDS             12955..13905
                     /locus_tag="U4B29_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN2106820.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="U4B29_001876"
     CDS             14046..14798
                     /locus_tag="U4B29_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106821.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15878
                     /gene="queG"
                     /locus_tag="U4B29_001877"
     CDS             14817..>15878
                     /gene="queG"
                     /locus_tag="U4B29_001877"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN2106822.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDV"
BASE COUNT         5135 a         2986 c         3202 g         4555 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgta
//
LOCUS       DASJTM010000059        15830 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJTM010000059 DASJTM010000000
VERSION     DASJTM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.059"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..622
                     /locus_tag="U4B29_001878"
     CDS             140..622
                     /locus_tag="U4B29_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106823.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            819..1709
                     /locus_tag="U4B29_001879"
     CDS             819..1709
                     /locus_tag="U4B29_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN2106824.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2034..4505
                     /locus_tag="U4B29_001880"
     CDS             2034..4505
                     /locus_tag="U4B29_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2106825.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4608..6344)
                     /locus_tag="U4B29_001881"
     CDS             complement(4608..6344)
                     /locus_tag="U4B29_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN2106826.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6341..7045)
                     /locus_tag="U4B29_001882"
     CDS             complement(6341..7045)
                     /locus_tag="U4B29_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2106827.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7176..7679
                     /locus_tag="U4B29_001883"
     CDS             7176..7679
                     /locus_tag="U4B29_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN2106828.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7639..7977
                     /locus_tag="U4B29_001884"
     CDS             7639..7977
                     /locus_tag="U4B29_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN2106829.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7998..9416
                     /gene="ffh"
                     /locus_tag="U4B29_001885"
     CDS             7998..9416
                     /gene="ffh"
                     /locus_tag="U4B29_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN2106830.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9528..10106
                     /locus_tag="U4B29_001886"
     CDS             9528..10106
                     /locus_tag="U4B29_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN2106831.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10110..10631
                     /locus_tag="U4B29_001887"
     CDS             10110..10631
                     /locus_tag="U4B29_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2106832.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10710..11264)
                     /locus_tag="U4B29_001888"
     CDS             complement(10710..11264)
                     /locus_tag="U4B29_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2106833.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11517..11792
                     /gene="rpsP"
                     /locus_tag="U4B29_001889"
     CDS             11517..11792
                     /gene="rpsP"
                     /locus_tag="U4B29_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN2106834.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11804..12055
                     /locus_tag="U4B29_001890"
     CDS             11804..12055
                     /locus_tag="U4B29_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN2106835.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12180..12971
                     /locus_tag="U4B29_001891"
     CDS             12180..12971
                     /locus_tag="U4B29_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106836.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13141..13662
                     /gene="rimM"
                     /locus_tag="U4B29_001892"
     CDS             13141..13662
                     /gene="rimM"
                     /locus_tag="U4B29_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN2106837.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13652..14413
                     /gene="trmD"
                     /locus_tag="U4B29_001893"
     CDS             13652..14413
                     /gene="trmD"
                     /locus_tag="U4B29_001893"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN2106838.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14549..14896
                     /gene="rplS"
                     /locus_tag="U4B29_001894"
     CDS             14549..14896
                     /gene="rplS"
                     /locus_tag="U4B29_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN2106839.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15028..15789)
                     /locus_tag="U4B29_001895"
     CDS             complement(15028..15789)
                     /locus_tag="U4B29_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2106840.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDY
                     KRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5207 a         2858 c         3213 g         4552 t
ORIGIN      
        1 aattactttt tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa
       61 aaaaagtttt tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa
      121 gtagtttggg ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt
      181 ttggtttttt cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc
      241 ggagatatta ggcctgatcc aaggaagcgg atttgtggtt tctaggaatt ttccagcttt
      301 gactgttttc tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca
      361 ctacaattgg cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt
      421 gctgcctcgc ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc
      481 ttattcctcc atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt
      541 tattcttgca acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt
      601 atctataaaa aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa
      661 aatgcatgtt tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca
      721 tgatttttac ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac
      781 tgataaactt tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc
      841 taggtatgat gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct
      901 ctgccacaca aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt
      961 cgacagcatt acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa
     1021 attatcaaat ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg
     1081 gagcagttac tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt
     1141 caaaattagt ggatcataaa gtagcagtgg tcgggatggc gccaatcgtc aataaagata
     1201 cagatgtcaa agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta
     1261 actggaagga agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca
     1321 gcggtacccg cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc
     1381 aagaacaaga ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca
     1441 gctatttagc tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag
     1501 aaccaaaaga agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt
     1561 acacgaacgg aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag
     1621 aaatccaaga aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag
     1681 aacgtgatca cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta
     1741 ctattaagga aaaatcaact atgtttccca catatacatt tttcctttaa ttgtgaaaaa
     1801 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttaaacaccg
     1861 tttattcgaa aaaaagagct acgacataga actgtcgtag ctcttttttt gctatatgac
     1921 aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct gttctatact
     1981 ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga
     2041 ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca gataaagtca
     2101 tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag gaacgaatag
     2161 aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag caaatcgtat
     2221 tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt ttgctttgtt
     2281 tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat acaggaatcg
     2341 ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt ttaaatacag
     2401 caaaagtcaa tgtcatacgg gacgggatag aacaaacaat agatatagaa gaccttgtaa
     2461 tagatgacat tgttatttta gagactggac atcaaattcc agcagatgca gaggtcgtgg
     2521 aaggaaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa ataaccaaag
     2581 aaataggcga tccattaatg tccggcagtt tcattgtttc cggaaaagct tacgcccgat
     2641 tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc aagacaatgg
     2701 gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa tggattggga
     2761 tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt aatggaaaca
     2821 cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata cctgaaggac
     2881 tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca aagcaaagag
     2941 ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc ctttgtgtag
     3001 ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaagtgatc gtctctaaaa
     3061 aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac tatgcaaaag
     3121 cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca aaaaccactg
     3181 atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc agtgtagcgt
     3241 tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg
     3301 aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg
     3361 tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca ttaggctata
     3421 ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac ttcaaaaaac
     3481 agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc aatgtagctg
     3541 tccaagcagg gatttcacat gctgatcagt acgttgatgc atctaaatta tcagaagaag
     3601 aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca gaacaaaaga
     3661 aaatcttcgt tcaattgata aagcgtaaca atacagtagc tatgacaggt gatggcgtga
     3721 atgatattct agccatgaaa gaagcagact gcagtatagc gatggcttcc ggaaatgaag
     3781 ccgccatgca ggcctcacaa gtggtcttac ttgattctga cttttcacgc atgccagaag
     3841 ttgttgcaga aggacggcgc gttgtgaaca atattgagcg atcagccagc ttgtttttag
     3901 tgaagaatat tttctccttt ttgctctcac tgttttcagt gatctttgct ctgacctatc
     3961 ctttagaacc atcacaaatc acattgatta gtttgtttac tatcggactt ccatctttct
     4021 tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg
     4081 aaaaagctgt gccaggtgga ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg
     4141 gggtgacttt gaatctaaat aaaacagatg tttctacagc atctaccatg cttttgattg
     4201 cagtagggtt cctcgttctc tataaaatct gttcaccgct gaataaattc agaagtcaga
     4261 tcattctttt ctgtgctagc ggtaactttt tttcggtcat ctttttgcat aagctgtttt
     4321 caatcaccgg tatctcagct gtatcacttt tgctgttgag cattctattt ttctcggcag
     4381 attcgatttt taggcatttg accacattgg tagaatattt gtttgaacgt aaaaatgaaa
     4441 aagagcaaaa gccgtttagt cgcgtagcta aaaaatttct atcgatattt aaacgagaaa
     4501 attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa
     4561 gcaggatgag tcatccaact ttaaaacgca cgagcagttg ctctttttta cattttcgga
     4621 atggtaacgg tgaaagtcga acctgttcca agatgactgt ctacagtaat cgtaccatta
     4681 aggagttgca cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc tgaatgtcgg
     4741 cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg
     4801 atacctatac caaagtcttg gacagaaaac gatagatcat ctgttgcctg atagcggata
     4861 ataatctcgc tgtcgctttt tgaatattgg acagcatttt caatcagatt tttgataatt
     4921 ggataaagga tatctgtttt agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc
     4981 aaccgtttct tttcgatgat tggctgataa ttttgaacga tttgctggaa ataaggttct
     5041 gtataaattg tctggatctc gtaagaattc tcgccatctt ttgacagctg gatgatttcc
     5101 cgaatcagtt tatctaaccg gatcgcatct ttttgcatga tctcaagaaa agaagtcaga
     5161 gtttgcgggt catcttttgc accatccagt aaagtctcgg taaaaccaat cagtgaagtc
     5221 acaggagttt ttaattcgtg agagacgttg ccaacgaaat ccttttgtag cttttctaaa
     5281 cgtcgcactt tcgttaagtc atacgccacc cctagaatct gtccggtacc ttctgaatta
     5341 gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt gatctcctga
     5401 tggatcagcg gatgctcagg agtcacttga tgaatcagct gaatgagttt tggctcttga
     5461 atgacttctg tgtaccgctg ttcaggttga tagcttacca cgtttaaatg tagctgcatc
     5521 tttggattaa gcagtagtaa acgagaatca gcatcatcaa tcaggaatac gccaatcatc
     5581 agttcgttca gtaacgtata taattgctct tctgtggtag tacacgcccg atacatctta
     5641 ctcatttgtt cgctcaacgc attaatcgtc tgatacaatt cttcccattg atcgggtgtt
     5701 tgcatgatta cttccgcctg attaggattt tgcaccattt tttttagtac gggcagcacg
     5761 gttttcaatg gctggttttt ttgatagatc agataataga tcattcctgc aagaacaagg
     5821 aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa
     5881 ccgcttgtcg gttctgcgat acgtaaaatg gcttctaact ggccactttt tttgactggt
     5941 aatgcgacat ataaaagatc ctcattcaaa gtagtacttt tgcgcaaagc gctacctagg
     6001 ttcccaccat tcaatacggc tttgatttcg ggacgggagt ttcgctgttc attcaatgtt
     6061 tcattgtttg tgtcaaataa gatctttcca gtagcatcca ttaaagtaat ccgttcgttc
     6121 gactgatgga cgaattcttc caaagcagtt ttgttttgcg ctgcttggag gttttcgaca
     6181 tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt agctttcttg ctgttccacg
     6241 atctgctgtt gaaaataatg ggaaatcaat tgccagccac caaaaaacaa actaactaaa
     6301 agaataagaa agaagagacg ttcataaatt tttttccatt tcatcgtttt ggttcctgaa
     6361 acttatagcc aaatcctcta atagtcacca agtactttgg atgttttgga tctcgctcga
     6421 ttttttctcg caaatgacta acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa
     6481 aattccaaat gcggtctagc aatgtgtctc ggtcgatcac acggtctttt cgtttcatga
     6541 aataaacaag taattcaaat tctttcggtg ttagttcaat cagatggtcg tctacggtca
     6601 cttgataatt ggtcaaatca gcagtgattt gtccaatcga tagatattca ggctcagctt
     6661 catcttttga ttgcttacga ggttcaatcc gtctgaatat tgctttcata cgggctaata
     6721 cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc acctatttct aggccaatga
     6781 tccgatccac ttggtcatct ttagccgtca gcatcaaaat cggtgtttca tttttttctt
     6841 gacgcagccg ctgagtgatc tccatgccat ccatcgttgg aagcatcaca tctaaaatga
     6901 tgaaatcaaa tgattgttct aatgctaatt ctaatccttc gcctccatct gtggcactgg
     6961 ttaccttgta gccttctttt tccaaattga acgtcaagag cgttacaata gaaggttcat
     7021 catcaacaac tagtattttt ttcattcaaa tctcctttaa ataggtttca ttctgtttat
     7081 attttagcat agaatcagta caaagggcta ttttacaaaa gaaataaatg gtataatagg
     7141 aaggcacttt gctgaataga aaggaagagc agtcaatgat tggaatcagt gcatgtttag
     7201 gcggtctggc ctgtcgctat gatggacaga aaaaagaagt accagaattg atggaactag
     7261 ttgaaactgg agaagctgtc atggtctgtc cagaagtgat cggcggatta ccaattccta
     7321 gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat caagcaaaag
     7381 tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct
     7441 atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa agcccttcat
     7501 gcggaaccca ttcgatttat gacggaagtt tttccggaat aaaaaaaaat ggtgccggag
     7561 tggctactgc ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga
     7621 aaactagagg tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa
     7681 ttttattcga ccttattgac agaaaaacaa atgaactaca tggaattata ttatgcagat
     7741 gatttttcat taggtgagat cgcggaagaa tacgaagtaa gccgacaagc agtatatgac
     7801 aatatcaagc ggacaagcaa gatcttggag gattatgaaa aaaaactcca tcttttttct
     7861 aattatattg ttcgagaaca ggtattggaa aatatgactg catatattac agataagtac
     7921 ccggaagata aaaaactact tgaatatgta cagcaaattc aagcaatcga agaataaata
     7981 aaaggaatga agaaaatatg gcatttgaaa gcttaactga acgtctccag caggcgatgg
     8041 gaaaaataag aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa
     8101 tccgccttgc cttactagaa gccgatgtta acttgcaagt agtcaaagat tttactaagc
     8161 gcgtacgcga acgtgcggta ggggcagaag tgctggaaag tttatcacct gcacaacaaa
     8221 tcgtaaaaat cgttgatgaa gaattgacga tcacacttgg ttctgaaaca gcagagttaa
     8281 ataaatcacc aaaaatacca actgttatca tgatggctgg tcttcaaggg gcaggtaaaa
     8341 cgacctttac tggtaaatta gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc
     8401 taatcgcagg ggacgtatat cgaccagctg cgatcgacca attgaaagta ttaggtcaac
     8461 agctagacgt tcctgtattt gatatgggaa ctgaagtcag cccggtagag atcgtccgtc
     8521 aagggatgga gttagcaaaa gaaaagaaaa acgactatgt attgatcgat acggcaggac
     8581 gtctgcatat cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac
     8641 caaatgagat tttgcttgtt gtcgatgcca tgactggtca agatgccgtc aacgtagctg
     8701 atagcttcaa ccagcaatta ggtattacag gggtcgtcat cactaaactt gatggggata
     8761 ctcgaggcgg ggcagcacta tcgatccgct cagtgacagg cgcaccaatc aaattcatcg
     8821 gttctggtga aaaattgact gacttagaag tattccaccc agatcgtatg gctagccgta
     8881 tccttggtat gggggatatg ctgacattga ttgaaaaagc gcagcaagac tacgacgaga
     8941 aaaaagcgga agaacttgca cagaaaatgc gggaaaattc attcgatttc aatgacttta
     9001 ttgaacagct tgatcaagtg atgggtatgg gcccactgga agatctaatc aaaatgatcc
     9061 caggaatgaa tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac
     9121 gcaaaaaagc gatggtttat tctatgacgc cagcagaaag agcaaatcct gatctgttga
     9181 acccaagccg tcgtcgccga attgctgccg gttctggaaa cagtgttgtt gaagtgaatc
     9241 gaatgatcaa acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata
     9301 tgaacatccc aggtatggac caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa
     9361 tggcgatgaa tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg
     9421 aaaaaaacag cttcggtaca gactgaaaat gtctactgcc ggagcttttc tatcagcaat
     9481 ttttattctt tactaataca attactctaa acgaagaggt ggcacagatg agatgtccgt
     9541 ggagtgaaca aacagaaagt atgcggcaat atcatgatac tgtctggggt gttcctgaat
     9601 atgatgacca aaagctgttc cgaaaattga tgcttgatat taatcaagcc ggattgagct
     9661 ggcagacgat tttaaataaa agtgaagcat tcgatgaagc atatgatcga ttcgatatca
     9721 aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta
     9781 ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc
     9841 aagaagaaat cggcagtttt tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg
     9901 attcttctta tcattcacaa gaagaagtcc caacaacaag tcctctttca gatgaattag
     9961 caaaagatct aaagaaaaga ggatttaaat ttatcggaag cacaacaatc tatgcttttt
    10021 tagaagctgt cgggatcatc aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg
    10081 tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat catattttat ggtgctataa
    10141 gtttagatgg ctatttggca acgaaaacgg atgatcttca atggcttttt gacactccaa
    10201 caggtaagca aactacttat gaagcttttt atgaaacaat cgatacgacc atcatgggca
    10261 gaaaaactta tcaggaagca aaaaaatatt tggagaccga agcaatttat ccagagaaaa
    10321 agaatttcgt tttttccacc cacgcacaat tacaattgcc agatgctgag gtcgtaaacg
    10381 aagatcctgt tgcttttcta aaaaatctac aaatgacaga aggaaaagat atctgggttg
    10441 tgggtggcgg tcgattgatc aaaccgctat tggaacatca aatgattgat gaatggtata
    10501 tccagattac ccctgtatta ttaggggacg ggattccttt attccaaaaa ggaacatatg
    10561 aagcgcgttt tgaaatgcta gacacgacac gattcggtga atttatcgaa ttgcattatg
    10621 tacgaaagta gaagtaaata aaataaaaaa ggcaactata tgcagaaatg tcctgtacat
    10681 agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga aaaatctaga
    10741 tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt accattttca
    10801 tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt tggcacattc
    10861 aaaaagacaa gtgattggcg tagatggtgg tttgcgccaa agccgccgat cccacctggt
    10921 gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata aggtcttgag
    10981 cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc aggagtaacg
    11041 aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc agcaggagcc
    11101 tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt tacaaataca
    11161 ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt gttgtaagaa
    11221 tcttttctta aactaccaat aaaaaatcca attttttttg tcatacgatt tttcctcctt
    11281 caaactatct ataataatct tacaaaagat aagtaactgg agcaaataaa aacacttact
    11341 gacaaaagct acatagaaga tagattactt atttttaaca aaaaagaagt ctaaaatctt
    11401 ctattttcta aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct ttacagggta
    11461 aaaaaaaact gttacactac atcttgtgaa taaaacaatg gaggtggaaa taaataatgg
    11521 cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg
    11581 tagctgattc acgttctcct cgtgatggac gtttcatcga aactgtaggt acttacaatc
    11641 ctttgaaaga ccctgcagaa gtagttttaa aagaagactt agttcttgac tggttatcaa
    11701 aaggtgcaca accttctgac actgtacgta acatcctttc aaaagaaggc gttatgcaaa
    11761 aacaccacga agctaaattc tcaaagaaat aaggtgaccg gatatgaaag atttaagcga
    11821 cttagtctta acaatcgttc gtccgctagt cacttatcct gatcaagtca agttagacgt
    11881 cgtagagtcg aaagactttt acgagtacaa tctaacagtg gctcctgaag atattggccg
    11941 tatcattgga aaacaagggc gtgttgctaa agcaatccgc acaatcgttt acggtgttcg
    12001 gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa
    12061 cgtgctgtac tcgaattttc gagtgcagct tttttgtttt ttttattaat tgaagaaatg
    12121 attatttggc aaaacaagca agaaagcgta tccttatagg aggagggagt gaacagccaa
    12181 tgagaaagaa aatgttattt attccatttg ctctattgtt aagcgcttgt agttcggtta
    12241 ctcaagaatc agacaataca catctatcaa gcagtatatc tgaaactgtt accagtccag
    12301 aaaaaagtag taccaaaacg gcttccacca ctgaacaaac aatggctacc tcaaataatg
    12361 agaaaaagac tgctttagac cagttgaaag aacaacagcc aaatgttccc atgcctctag
    12421 atgtaccagt ctcttcaggc tatctcaata tcgcagcgac acatacaaag caaggttatt
    12481 ctattttata ttacagaaca gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag
    12541 aaacgcctat tgcaacctat ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc
    12601 aagttttgca gccatttgag atcgacacaa atggtcagca agtggactta ggttctcgaa
    12661 tcacagggta tcagcaaggt gcagcaggtt caagttttct tgaatggcaa gaaggaaact
    12721 ggcgtattcg aatccgtggg aataatatag aaggccaaga tcccttacta ttagctaaag
    12781 aaatcgtagc ttatttggaa gaaaacagtc taccagcacc tgaacaattt ggtaaaatta
    12841 ctgttgatat gggtgatacg acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa
    12901 atgcctatac gatcactcat caagatccta tgtctgcgtt gaagatggca gtttctatga
    12961 agaggttgtg aaatcagcgc gctgatctga gcccatcatt taactccaag tcacaggaag
    13021 taatcaaaaa tagcacgccg catttttgca taattttggc ttaatacctt aaaagaaaac
    13081 tagaattgcc tcttcacaca tgctaaaata aaacagacaa aaaagaaatg gggatataag
    13141 ttgactgaat acttgaatgt aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc
    13201 cgtgttatct caacaacgga tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca
    13261 ctattccaag acggaaaagc acctgtggaa ttaacagtca aaagtcatcg caaacataaa
    13321 aattttgact tattgagttt tgaaggacat ccttctatca atgatgtgga aaaatatcgg
    13381 gatggcatat taaaagtgtc aaaagacaac tctgtagatc tagctgaaaa tgaattctat
    13441 taccatgaga tcatcggtct gcatgtatta gaagatggaa ctgagttagg taaagtaaaa
    13501 gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat
    13561 atattgcttc catatatcgc ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc
    13621 cgtgtagaaa ttccagaagg actgattgac gatgaagatt gatgtattga ctttatttcc
    13681 aagaatgttt gaaggtccta tgggggaatc catcatcggt aaggctgtag ataaaggatt
    13741 attagatatc aatatctcta acttccgcga ttattcagat aacaaacatc agacagtaga
    13801 tgattatcca tacggcggtg gagcaggtat gctgctcaaa gtccagccca tctatgataa
    13861 catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc
    13921 agctggtaaa cgattcgatc agaaaatggc agaagaattt tcacaagaag aacacttagt
    13981 attcatttgt ggtcattatg agggatatga tgaacgtatc cgcacattag tgacagatga
    14041 agtttcctta ggagattatg tccttactgg gggagaatta ggtgcgatgg tcatgatcga
    14101 tgcaacagtt cggcttttgc cagatgtgtt agggaatcag acttcagctc aaacggattc
    14161 ttattcaacc ggtttattgg aacatcccca atatactcgc ccggcagaat ataaagggat
    14221 gaaagtacca gaagtattga caaatggaaa tcataaattg attgaagaat ggcaattaaa
    14281 agaatcacta agaaggacct atcaacgtcg gcctgatctt ttggaaacac ttgaatggac
    14341 acctcaaatg acgaaatttt tagaagaaat caagaaagaa gaacaagaaa aaacagcaga
    14401 tgaagccaag tgactttttc taaaatcttc tttacagtag gttgataata tgttagtatt
    14461 ttatggtgag tgcaaaagca cttaactatt acgatattcc gctgtgagaa aagctcataa
    14521 gaatatttgg aataaggaga agaaaaccat gaatccatta atcgaagaat taacaaaaga
    14581 acaattacgt tctgacatcc cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa
    14641 agttgttgaa ggtactcgtg aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg
    14701 cggtgctgga atcagcgaaa cttatacagt acgtaaagtt tctaacggag tgggcgttga
    14761 acgtacattc ccattgcata caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa
    14821 agtacgtcgt gcaaaattgt actacctacg tgcattacac ggaaaagctg ctcgtatcaa
    14881 agaaatccgt cgttaatcat cgttatcaga cagagaaaga acccttggta ttcaagggtt
    14941 ctttttttat tatctgtttt tttatattaa cgatttctat ttggtacatt tttgattaat
    15001 aaaaaggggc aaaaaagggg caacaagtta cagtttttct atatcttctt tcaatttttc
    15061 ttttactcct tttgtcacat gcaaataaat tttttcagtg atatcactat tttcgtgacc
    15121 aacgcgatcc tgaatggcat acagaggagt tcctagttcc gctaattttg aaatatgggt
    15181 atgtctaaaa atatgtgaac taagtttttt atcaatcttc atgtcagctt tatgacttct
    15241 caaataggtg ttaattgctg ttagttggaa aggagttcct ttcgttgttt gaaatagaaa
    15301 ttgaccattt ggatttaatt ctagaagctc attgtaaata gctatcgctt tttttggtaa
    15361 atcaatttct cgcattccgg cagctgtttt tgtagaatca gattttttca tatcagctat
    15421 tgaacgttct ctatacatca tcgtcccatt tataactact gatgcattat tattggtaat
    15481 gtgtacatca tctttactta atgcgattgc ttcaccagac ctcatcccag tcaaatatag
    15541 ccactggaaa aggagagaat atcttttatt gtgtgaagtt gtgaaatcta ctagtctatt
    15601 atattcatca tcttctaaaa atttatcttt aatttttatc gtttttgact ctcttttata
    15661 atcaataact acttcatcga taggattttt ttctacataa ccttttttca tagcatatga
    15721 aaaaagaaga ttcaatttgg atttaattac actgacgtac ttatttgaca agtcatcttt
    15781 atatatcatg tcttcaaaaa tattattcaa atctattgta ttaataccag
//
LOCUS       DASJTM010000060        15761 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJTM010000060 DASJTM010000000
VERSION     DASJTM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.060"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="U4B29_001896"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="U4B29_001896"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN2106841.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="U4B29_001897"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="U4B29_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN2106842.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="U4B29_001898"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="U4B29_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN2106843.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="U4B29_001899"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="U4B29_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN2106844.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="U4B29_001900"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="U4B29_001900"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN2106845.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="U4B29_001901"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="U4B29_001901"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN2106846.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="U4B29_001902"
     CDS             complement(8557..9027)
                     /locus_tag="U4B29_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN2106847.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="U4B29_001903"
     CDS             complement(9073..9498)
                     /locus_tag="U4B29_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN2106848.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="U4B29_001904"
     CDS             complement(9565..9834)
                     /locus_tag="U4B29_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN2106849.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="U4B29_001905"
     CDS             complement(9845..11440)
                     /locus_tag="U4B29_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2106850.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="U4B29_001906"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="U4B29_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN2106851.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="U4B29_001907"
     CDS             complement(14993..15553)
                     /locus_tag="U4B29_001907"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN2106852.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         4091 a         3523 c         2770 g         5377 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa t
//
LOCUS       DASJTM010000061        15742 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJTM010000061 DASJTM010000000
VERSION     DASJTM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.061"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="U4B29_001908"
     CDS             complement(24..722)
                     /locus_tag="U4B29_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN2106853.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="U4B29_001909"
     CDS             complement(715..2067)
                     /locus_tag="U4B29_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN2106854.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="U4B29_001910"
     CDS             complement(2290..3690)
                     /locus_tag="U4B29_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN2106855.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="U4B29_001911"
     CDS             3851..4588
                     /locus_tag="U4B29_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN2106856.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="U4B29_001912"
     CDS             4713..5192
                     /locus_tag="U4B29_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN2106857.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="U4B29_001913"
     CDS             5189..5956
                     /locus_tag="U4B29_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106858.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="U4B29_001914"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="U4B29_001914"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2106859.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="U4B29_001915"
     CDS             complement(6859..7461)
                     /locus_tag="U4B29_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2106860.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="U4B29_001916"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="U4B29_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="U4B29_001917"
     CDS             8142..8726
                     /locus_tag="U4B29_001917"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2106861.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="U4B29_001918"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="U4B29_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN2106862.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="U4B29_001919"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="U4B29_001919"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN2106863.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="U4B29_001920"
     CDS             10627..10806
                     /locus_tag="U4B29_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106864.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="U4B29_001921"
     CDS             10828..11853
                     /locus_tag="U4B29_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN2106865.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="U4B29_001922"
     CDS             12028..13272
                     /locus_tag="U4B29_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN2106866.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="U4B29_001923"
     CDS             13425..14372
                     /locus_tag="U4B29_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2106867.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="U4B29_001924"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="U4B29_001924"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN2106868.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="U4B29_001925"
     CDS             complement(15274..15516)
                     /locus_tag="U4B29_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106869.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="U4B29_001926"
     tRNA            15637..15709
                     /locus_tag="U4B29_001926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DASJTM010000062        15597 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJTM010000062 DASJTM010000000
VERSION     DASJTM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.062"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            143..529
                     /locus_tag="U4B29_001927"
     CDS             143..529
                     /locus_tag="U4B29_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2106870.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            623..814
                     /locus_tag="U4B29_001928"
     CDS             623..814
                     /locus_tag="U4B29_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106871.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1328..1660
                     /locus_tag="U4B29_001929"
     CDS             1328..1660
                     /locus_tag="U4B29_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2106872.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1956..2111
                     /locus_tag="U4B29_001930"
     CDS             1956..2111
                     /locus_tag="U4B29_001930"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN2106873.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2387..3727
                     /locus_tag="U4B29_001931"
                     /pseudo
     CDS             2387..3727
                     /locus_tag="U4B29_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3968..5584)
                     /locus_tag="U4B29_001932"
     CDS             complement(3968..5584)
                     /locus_tag="U4B29_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2106874.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5702..5920
                     /locus_tag="U4B29_001933"
     CDS             5702..5920
                     /locus_tag="U4B29_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106875.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6115..6576
                     /locus_tag="U4B29_001934"
     CDS             6115..6576
                     /locus_tag="U4B29_001934"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2106876.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6769..7008)
                     /locus_tag="U4B29_001935"
     CDS             complement(6769..7008)
                     /locus_tag="U4B29_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2106877.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7032..8555)
                     /locus_tag="U4B29_001936"
     CDS             complement(7032..8555)
                     /locus_tag="U4B29_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2106878.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8573..8695)
                     /locus_tag="U4B29_001937"
     CDS             complement(8573..8695)
                     /locus_tag="U4B29_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2106879.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(8725..9708)
                     /locus_tag="U4B29_001938"
     CDS             complement(8725..9708)
                     /locus_tag="U4B29_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN2106880.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9732..9881)
                     /gene="rpmG"
                     /locus_tag="U4B29_001939"
     CDS             complement(9732..9881)
                     /gene="rpmG"
                     /locus_tag="U4B29_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2106881.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9902..10279)
                     /locus_tag="U4B29_001940"
     CDS             complement(9902..10279)
                     /locus_tag="U4B29_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106882.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10311..10490)
                     /gene="rpmF"
                     /locus_tag="U4B29_001941"
     CDS             complement(10311..10490)
                     /gene="rpmF"
                     /locus_tag="U4B29_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2106883.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10505..10774)
                     /gene="rpsN"
                     /locus_tag="U4B29_001942"
     CDS             complement(10505..10774)
                     /gene="rpsN"
                     /locus_tag="U4B29_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2106884.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11297..13063)
                     /locus_tag="U4B29_001943"
     CDS             complement(11297..13063)
                     /locus_tag="U4B29_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106885.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13023..14777)
                     /locus_tag="U4B29_001944"
     CDS             complement(13023..14777)
                     /locus_tag="U4B29_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106886.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(14887..15456)
                     /locus_tag="U4B29_001945"
     CDS             complement(14887..15456)
                     /locus_tag="U4B29_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN2106887.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         5026 a         2989 c         2373 g         5209 t
ORIGIN      
        1 catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc
       61 gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat
      121 cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat
      181 tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga
      241 aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat
      301 aaaatttaaa caaccgatta atgttttaaa tgtgactctc cttgttgaag atataaaatt
      361 agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg aaaaatttat
      421 ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac agggtgcgac
      481 aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa
      541 taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat agattataag
      601 tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt tagcaaaggc
      661 tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga ttcataagca
      721 atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata
      781 tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa
      841 gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt tataattata
      901 aagtatcgat tttacagcgt tctataatga ttcatagaat ctaaaacgaa tgattttaat
      961 tatcaacgtt ttttaattcg tttttagctt aaaaataact tttgtcacag tctcactcct
     1021 aaaattactt tatttacgta tgaagtgggc aggaggtcga atccagaact gggctatggt
     1081 acttcaacga ggacttctcc tctagaattg aaaagcatgc gatttacctt ccgtaacgac
     1141 ggtagattta ttcaactatg tgtccaatca aaatttgtct gatttcaaaa atagtcaaga
     1201 gaattttggt tgttaaaaac tcttatctat caacttccac agtttagttg acaaaccctg
     1261 atactaagtt gggctatgat acacttttct tgactatcag ttacgttacc ggaaggaatg
     1321 aactctattg agaaaaacat atgatcgtaa atttaaacta aagatttcac aggatatcct
     1381 tgagaaaaaa gtaactacca agaaaattgc tgaagaatac aatatctctc gtccaacaat
     1441 ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat gcttttgctg gacaaggaaa
     1501 aagattacct gataaagcag atttctacat tcttgaacag gaaaataaac gactaaaaga
     1561 ggaagacgag tgttctacga acacaaagat cgatacggat ctccagggat cactcaaaaa
     1621 ctttacgatg aaggaatcga aatcaataaa cgtgttgtag ctgttcttat gcgtgaaatg
     1681 aatttatgca caagaggatt tcatcgacgc aaatcttctc atggtagaga aaaaacaatc
     1741 aaagaaatgg taaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt
     1801 gggtgatgga tattacttac attccatgta atgacggtcg cctctatttg tccacctata
     1861 ttgacttagc aactcgtatt ctacgttact atatggtaga ttcacacatg aaaaaagaga
     1921 ttgtcgtccg tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata
     1981 gaggtagcca gcatcggtct tttatttatc aagagctgtt gaacgaccac cacatcacac
     2041 attcagtgag ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat
     2101 caatcaaatg agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt
     2161 taatccaaga ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga
     2221 tgactccttg acaatttgaa gcgaagaaaa taagggatct ataatatcta gtgttggtga
     2281 cgccttctgt tgccaatact ttttcttttt tctaataaac gcaaacagac accttaaaat
     2341 cctttagaat aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt cctattcaga
     2401 gtttaccaaa gagttactag atattctaga cctaaatctt atctttcatg aagatgcctt
     2461 tcgcaaagag cgaatcaatg acgaaacatg ttttgttttt gatggcacat tgacctatca
     2521 accggaggaa tgctttcatc gccactatca aaatgaacaa acgatcatta aatagggctg
     2581 gaaaaaagta tccatcttat tgaatgatgt cagtaactac aagaccattc tgcggattaa
     2641 caaacagcgc ttcaaatgta aacactgtgg aaagactttt ttagcagaag attcagttag
     2701 tgatcgtcgc tgctccattg ctcgcagagt caaacaagcc attcttgagc tattaagcga
     2761 acctctttct atgtctttga tcgcacgtat gaagcacatt tctccgacca ctgtcattcg
     2821 aatacttcgc agccttcggc ctaaaaccat ttctttgagc cagctgttac cagaagtggt
     2881 gtgtttcgat gaattcaaat cagtgaaaaa tgtctcggga gccatgagct ttgtcatgat
     2941 ggacggcaag acccatcaac tgattgatat tgtggaaaat cggcaactca atcctttacg
     3001 cgactatttc ttgcgctacc ctataaaagt ccgtgaacaa gtgcgtctag tggtttcaga
     3061 tttctataca ccctatcgca cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc
     3121 cgatcgcttt catattagcc aacacattgg tcgggctttt accaaccacc gaattcaagt
     3181 aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat actggaaact
     3241 acttcaaaag aatgcgtggg aattgaaagg tcagcatcgc tactggcgtc cgtcatttcg
     3301 agatcatctg acagaagcag agatagtcga tcggctatta tcttatgatg attccttaaa
     3361 gcgaggctac gaagtctacc aggattttct ttccgttatc agaagacaag atgttcctga
     3421 attcgtcgcg ctcctaaaag aagactacaa ggagttgcca gagcactacc aaccagtgtt
     3481 cacgaccttc aagaaatacc aaacagaaat caaacgagcg ttgcgcgttc cctattccaa
     3541 cggccctata gaatgtttga ataaccatat caaagtacta aaacgaattg cctatggcta
     3601 tagaaacttt caaaactacc gagaacgaat ctttttatat cgaggaaaat attttaagaa
     3661 aactaagaat actacccaac tgactaaagc cagaacaaca acacggctag acaagatagc
     3721 tgtctagccg tgtgagtatc tcgattattc tattgaattt cttcaccaac actcattgac
     3781 gaagagccaa aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa
     3841 gatacttgtg cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa
     3901 taaagcccct gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat
     3961 ttccagatta gtaaatgtag taatctgtgc tttctttttt ctcaatacta ataactaact
     4021 tatggggtaa acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga
     4081 tttgatcagg actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat
     4141 tatattgtcc cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga
     4201 agcgatccat ttccttgcca tcaattcgaa cgatggcata tttgatagca tcttcagttg
     4261 tttcattctc cttttctata gagaatacaa ataagggaat aaaagaggta ataatcaaaa
     4321 tgccaataat tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg
     4381 atatctggct aaacatatta gactttttcc atggaaatgg tgaaattttt cggaaattat
     4441 ctgcattaag agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca
     4501 tcttcatacc aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt
     4561 ccttcaattc gggatttttt tgtataatca tttgacactc aattttaggt aaactagcta
     4621 cccaataatt taatgattca atacttttat agctacccga tgtttcaaag atcgtcgaaa
     4681 aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat
     4741 ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa
     4801 aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact
     4861 ctttgaacca gcccactgaa taggaagtct catctatatt aaaaagattt gtatttagaa
     4921 ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact
     4981 tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta
     5041 aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt
     5101 cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat
     5161 cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca
     5221 aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga attggtttgc
     5281 tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta
     5341 gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa
     5401 gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca acatttactt
     5461 cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat
     5521 ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt
     5581 tcatagggaa cctctttcta taccttgaat ttacacttta agtgcaacac ataaaaatga
     5641 gtcataatcg atatccaact agtataagtt tgtcgagaac actcctagaa ggaaaataat
     5701 tatgagccag tatcgtcatc ttactatact cgaacgagaa agaatctttc tgcttcacga
     5761 agaaggattt tctattagta taattggcaa cgacattcag catagcccgt ttactatttt
     5821 acatgaactg agaagaaaca agaaaaaaac tattcacctt ccaaagcgga aaaaaattat
     5881 gcttatcgaa gaaaaaaatg cggacgttat cacattctaa atgatccgtg agtttggtca
     5941 attgttcgtc atttatttgt cgattttcag tggtcgccag aggaaatctc gaatcgtttg
     6001 aagcatgaac atatggactt ttcaattagt tttatcacga tctaacgtgc aatttgcaat
     6061 gatcttttcg atacagaacc cctctcacac ggcaataaag gcctgatccg ttcattgcac
     6121 catcgaggaa aaacaagaca tacaaaaaat catgtcgagt ctcgtggaaa gatttgcctt
     6181 actcattcga tccatggccg tccaaaatcc gcagatgata gaagtgattt tggtcattgg
     6241 aaagccgata ttgtagctgg caaaactggt ggtacttgtt tagtgacttt gacagacaga
     6301 aaatcatgct tccttttagc aggaaagaag gtccccagaa agaaagccgc ttatgtgagc
     6361 gagaaaatga ttgaactttt aagcagtatt ccaaatgagg cggtcaaaac gatcactcca
     6421 gatagaggaa aagaattctc caatcatttc gcagttacag aagcattgaa taatatgccc
     6481 ttttattttc cagatcctca tgcccttggc aacaatgaac aaattaaaat acgaatagac
     6541 tcattcgaga atacttgcca aaatcgaaag agatagctac ggtagataac tcaacgataa
     6601 atcagatgat tgaaaaactg aataacagac ctagaaagtg tttagattgg agaacacctt
     6661 atgaagtctt ctttgataaa gtgttgcgct tgatttgaca attcaagaca taataaaaag
     6721 cagatcaaat ccaaataagg gaaaaatgat ctactctggt attatcaatt aattggccaa
     6781 gctattatgc cgttttgcta aacgtctact tcttccagta ttgaccttga taaaacaaca
     6841 atcaggatcc cgttttaata ctctatgaac tgtgtcaata ctaacatgta aagttgcagc
     6901 aatatcagct aatgtactcc catttaatct catttgcttt gcaaatcgat aacgccgata
     6961 agcatcaata ttcttggaat ccatagacaa taatactttt cttaccatgt atttcctcca
     7021 aaaattgaat tttaatgagc catcatttct tgagcaattt catgtttaga caatgaagct
     7081 ggatagaaag taggccaatt atgcaattca ttatagagtt tttcttgact ttctccacca
     7141 aagaaaatat ggaaatgctc tgccttactt ggtgcaattc catgatcact aaattgaata
     7201 tatttaaaac gaccagcacc tgcatcatca gtttcaaaat taaaacgaac accacggttt
     7261 cctttttcat aattcaaaat tttgtatcca acatatttgt atgtgtattg atgatgttca
     7321 ccgttgacta caaaatcaat ggtgttgtct ttaatgttga tagtatcaat atctgtctta
     7381 tagccagtag tataataatc tttgtactcg gcagctgtca tgtctttatt gatttttgct
     7441 ttgtaatcga aaacttcatc caaaattcca tcttttaaca acggataaac agattgccat
     7501 tcgcctgtat aatcactcaa ttcgcgatcc ttcacatcgg catctttaaa gtacccatta
     7561 gcaactgtct tttcatcctt cgcttctttt tctggaacta cctcttcacc agcaaccata
     7621 gttgtttttt ctaacgcttt tagattgtct tccattactg aaacataatc ttcaccatta
     7681 tccatttgtt cttttgtaag actttcaaga ggattcagta cttccaattt tatcccagct
     7741 tcgttggcca aagtttttgc aattttatca ttagcattct tttcaaagta aatataattg
     7801 atcttatttt ttttaacata ctcttttaat tcagctaatc gaccagaaga tggctcttct
     7861 tcaggactta gtccagcaat aggaacttgt ttcaatccat aatccaaagc taaatatcca
     7921 aaggctgcgt gttgcgtcac aaaacttttt tgtttcgctt ctttaagact agtggtatag
     7981 tcagcatcca accgtttcag tttagtcaaa tacttttcag catttgtctc aaatactttt
     8041 gcttttttcg gatacaattt aactaactga tccttgatat ttgaaacttc cttaattgcc
     8101 attttgggtg aaacccacgt atgaggatct agttcatgat ggtgatcttc ttctccgtga
     8161 tcatgatctt cttcttcact acctggcaat aaaatcatgt cttttgttcc ttctactaca
     8221 ttaggcccag ctttcttcca actttcttta gcttttggta cccatgattc catgttttcg
     8281 ttatgatata caaaaacatc agcatcatga gcttttgcca tatccttagc tgagggctca
     8341 tagtcatgag gttctgatcc cgctggaacc attaaatcca cgttcgcttc atcgccgacg
     8401 atgtttttgg tgaaatcata cattggatag aatgttgtta caatttctaa ttttccatct
     8461 ttatcattcg atacttttgc atcattagtg ctggagcaag ctcccaataa tcctagagct
     8521 gctatcccaa taagtcctac actgattttc ttcattcttt ttccttcctt aattatgcta
     8581 atttcttcat tttatttcgt ttatgttctt tgataatttt taatgccctt tttctagttt
     8641 taatattcgg gcttttaagt cgtcgatatg cactagatat atccgttttc tccattagaa
     8701 attaccccca atcactactt atcattattt gctttccgcc agtaatttat caaaactctg
     8761 tttgatcctt tcttcatcca acatttttcc gattagaata atagtggttt ggtctttttg
     8821 tcgagtggtg tattgaaaat taacctgttc gtttacctct tgcatggcaa ctataaaatc
     8881 ctgctcttgt aaagcaatca atcctttaaa acgatacagg ctcccttggt tggttaaaat
     8941 cagccagtca atccattgga gtaaaaaatc tttttttatg tcttgatctg aagttaagag
     9001 aattgattta aactcatgta cttgatggtg ttgatgatag tatttgccat tatagtgatt
     9061 ttctgatgaa tcactttcaa aaactaaggg atgattaaat tttttcaaat tataaaaatc
     9121 ctttttttga atcgtttcac tatgtgtgaa cattcgaata tcggataaag ggttaatact
     9181 tttgatttct ctctgtaatt tttttagtgc agataaattc aagttttgtt ttctagacac
     9241 aaaaatgcga tctgccatga caagttgctt aagggcttgt ggttctcttt caataagttc
     9301 aaaactgtca ctatcaataa cagtcaataa actatcgata tatactcttc ctcttagttg
     9361 aggcgctgtt aaaagtgttt gaatgattgg ttggggatct gctatacctg tagtttcaat
     9421 gatgatttgt gaaattggtt gttgatgttc ttctattgtt tccagtaaag aaatcaatat
     9481 tgctaataaa tcactgcgaa gactgcaaca catacatcca ttatttagtt gtaacacccg
     9541 ttcctcgcta tgaactaata atttgtgatc aatcccaact tcaccaaact cattttcaat
     9601 tataataatt tcttccctgg ggtagggtga gacttgcaag gcttgattga ttagcgttgt
     9661 ttttccagcc cctaaaaacc cagaaacgat tgtaactggt atgcccattt tgtcaccttt
     9721 ttaccactta gctatttcac ttctttaaaa attgcccgtc tgcgcaattt tggcgaatac
     9781 ttttttaatt ctaaccgttc agggttattc cgtttatttt tgctggtgag atacagacgt
     9841 tctccagttt cgacacattc caaaataata ttttgacgca taaatcttcc tccttcaata
     9901 cttatcgatc ttcaagatat gcttgaattt gttgtgcatc ttcaaaagtt aactcgccag
     9961 attccaattg tgaaactaaa ttttctttca tatcaacagg tatctcccaa taaccatcga
    10021 tacattgaaa taactcctcg tcatactctt caaaaattgg atagattctt aaaaaatcat
    10081 caaatgataa gccttggtcc tcatagtaat ctttgactaa tccattttca attgcctgtt
    10141 gggtcggtaa acccgtttca gtaataattc ctctctctac catcgaacga taaaaagtgc
    10201 atttatctag cgattgctgg gtcgtttcaa gataatactt taatccttct tcaactaaag
    10261 gtgggtaaat aatttccatt ttgattccct cctaaaagtt atatattaaa ctattttgaa
    10321 gctcctttaa ttactttctt tccattatag taccctttta aagatacatg atggctacgt
    10381 ctataatcac cattcgcttc gtcaaatgaa atttctggtt ttactacagt ttgatgagtt
    10441 cttcttaacc gtttcttcgc cttagatgtt tttcttgctg gtactgccat gatatttcct
    10501 ccttttacca acttgctttt tttactccag gaatcaaccc ttgatgggca agttctctaa
    10561 acttaatacg ggacataccg aactttcgca tataccctct tggacgacca tccgtctgat
    10621 cacgaagcct cagacgattc ggatttgaat ctcttggcaa tttagcgaga gcggtgcgat
    10681 ctcctgcggc ttttaattcc tgacgtttat ctgcatactt ttcaatcatc gccatttgtt
    10741 tctttgcttt agcaattttc gacttttttg ccatatacct ttatactcct tttattttga
    10801 tttagacacc tcaaatcgta atcgttacgt tttgacactg atactttacc aacaagagtt
    10861 gctcttgtca acaataaata acatgaaata aaactcatat gaatagataa ctatttgaaa
    10921 acgaatttat cggtacatag agataatcaa atcatttaaa agaatatgca gtttaggctc
    10981 tgaaaaataa gaattcactt ttattagtcc aatcaaacac aaataccgtt ttaagtagct
    11041 tttcctctac ttaaaacggc aatatgaaag aaactataac tttttattca atcttttatg
    11101 tttaactggt ttatcgccaa ttttttaaaa ctttgattat tccttatatt ataagaatag
    11161 acattaagtg aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt
    11221 ttaataaaat tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa
    11281 tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata
    11341 gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag
    11401 taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag
    11461 acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt
    11521 cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa
    11581 ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa
    11641 gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt
    11701 cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc
    11761 caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag
    11821 cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa
    11881 tacagccttc atcataatca tacagtctag acactaattt caagatacta gttttcccgg
    11941 aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat
    12001 ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag
    12061 atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct
    12121 gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat
    12181 cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg
    12241 ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag
    12301 aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct
    12361 cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct
    12421 cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat
    12481 atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata
    12541 acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa
    12601 taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct
    12661 caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa
    12721 aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt
    12781 ctttataagt actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa
    12841 gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta
    12901 attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa
    12961 aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt
    13021 ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc
    13081 aattgtttat acacttgatg tttagccatt aattcattat gatttccacg ttctactatt
    13141 ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct
    13201 atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag ttcatactca
    13261 ttatctggat caatagatgc actggcttca tccataataa caatctttgc atcttttaaa
    13321 attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta
    13381 ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat
    13441 cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg
    13501 taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt
    13561 gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca
    13621 ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact
    13681 aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct
    13741 ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc
    13801 ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca
    13861 tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata
    13921 attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga
    13981 gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat
    14041 tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa
    14101 tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata
    14161 acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga
    14221 tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac accaattact
    14281 aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat
    14341 aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt
    14401 gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa
    14461 aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag
    14521 cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt
    14581 aaccaagcaa cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt
    14641 agagactgaa gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa
    14701 tcagaaaata aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat
    14761 tttttgtata ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca
    14821 actgttaatg ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac
    14881 tacgttctat tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga
    14941 taaacttgct acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat
    15001 tgcatccatt gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt
    15061 aaagaaccat ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga
    15121 cgcaacttga ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat
    15181 cgccatacta atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa
    15241 taacaataaa acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat
    15301 ataaacaaat atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa
    15361 cgccccacca atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt
    15421 agctaaacca ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc
    15481 gttatgtcat taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct
    15541 ttttttattc caccattttt taaataaata attctattgc atgttttata aagaaat
//
LOCUS       DASJTM010000063        15506 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJTM010000063 DASJTM010000000
VERSION     DASJTM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.063"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..2188
                     /locus_tag="U4B29_001946"
     CDS             128..2188
                     /locus_tag="U4B29_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN2106888.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2271..2840
                     /locus_tag="U4B29_001947"
     CDS             2271..2840
                     /locus_tag="U4B29_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106889.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3015..3821)
                     /locus_tag="U4B29_001948"
     CDS             complement(3015..3821)
                     /locus_tag="U4B29_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106890.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3959..5473
                     /locus_tag="U4B29_001949"
     CDS             3959..5473
                     /locus_tag="U4B29_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN2106891.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5876..6859)
                     /locus_tag="U4B29_001950"
     CDS             complement(5876..6859)
                     /locus_tag="U4B29_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN2106892.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6864..7511)
                     /locus_tag="U4B29_001951"
     CDS             complement(6864..7511)
                     /locus_tag="U4B29_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2106893.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7596..8228)
                     /locus_tag="U4B29_001952"
     CDS             complement(7596..8228)
                     /locus_tag="U4B29_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2106894.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="U4B29_001953"
     CDS             complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="U4B29_001953"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN2106895.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8792..9529)
                     /locus_tag="U4B29_001954"
     CDS             complement(8792..9529)
                     /locus_tag="U4B29_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN2106896.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9526..10515)
                     /locus_tag="U4B29_001955"
     CDS             complement(9526..10515)
                     /locus_tag="U4B29_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2106897.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10773..11315
                     /locus_tag="U4B29_001956"
     CDS             10773..11315
                     /locus_tag="U4B29_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2106898.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11332..12417
                     /locus_tag="U4B29_001957"
     CDS             11332..12417
                     /locus_tag="U4B29_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106899.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12429..13235
                     /locus_tag="U4B29_001958"
     CDS             12429..13235
                     /locus_tag="U4B29_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106900.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13236..14063
                     /locus_tag="U4B29_001959"
     CDS             13236..14063
                     /locus_tag="U4B29_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2106901.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14060..15133
                     /locus_tag="U4B29_001960"
     CDS             14060..15133
                     /locus_tag="U4B29_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2106902.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         5003 a         2793 c         3146 g         4564 t
ORIGIN      
        1 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
       61 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
      121 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
      181 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
      241 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
      301 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
      361 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
      421 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
      481 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
      541 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
      601 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
      661 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
      721 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
      781 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
      841 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
      901 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
      961 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     1021 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     1081 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     1141 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     1201 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     1261 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     1321 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     1381 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     1441 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     1501 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     1561 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     1621 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     1681 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     1741 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     1801 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     1861 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     1921 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     1981 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     2041 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     2101 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     2161 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     2221 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     2281 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     2341 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     2401 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     2461 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     2521 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     2581 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     2641 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     2701 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     2761 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     2821 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     2881 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     2941 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     3001 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     3061 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     3121 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     3181 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     3241 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     3301 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     3361 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     3421 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     3481 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     3541 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     3601 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     3661 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     3721 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     3781 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     3841 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     3901 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     3961 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     4021 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     4081 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     4141 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     4201 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     4261 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     4321 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     4381 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     4441 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     4501 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     4561 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     4621 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     4681 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     4741 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     4801 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     4861 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     4921 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     4981 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     5041 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     5101 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
     5161 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
     5221 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
     5281 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
     5341 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
     5401 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
     5461 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
     5521 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg
     5581 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac
     5641 ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa
     5701 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat
     5761 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
     5821 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt
     5881 cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt
     5941 gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta
     6001 gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc
     6061 taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta
     6121 tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta
     6181 tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc
     6241 cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca
     6301 attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga
     6361 tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg
     6421 ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt
     6481 gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag
     6541 ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga
     6601 ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta
     6661 cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc
     6721 ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt
     6781 gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct
     6841 tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc
     6901 aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact
     6961 agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta
     7021 ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga
     7081 atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa
     7141 taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc
     7201 agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag
     7261 tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct
     7321 ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca
     7381 ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg
     7441 gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca
     7501 aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa
     7561 aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg
     7621 aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca
     7681 ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt
     7741 ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc
     7801 gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt
     7861 ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga
     7921 ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata
     7981 aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc
     8041 agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg
     8101 attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc
     8161 gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta
     8221 tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt
     8281 tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga
     8341 gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga
     8401 gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc
     8461 actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg
     8521 ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg
     8581 atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc
     8641 gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca
     8701 tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa
     8761 atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct
     8821 cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag
     8881 ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa
     8941 aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta
     9001 catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag
     9061 aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct
     9121 ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg
     9181 ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa
     9241 aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt
     9301 ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc
     9361 ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag
     9421 ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta
     9481 cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc
     9541 tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg
     9601 caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat
     9661 ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa
     9721 ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt
     9781 gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc
     9841 gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc
     9901 tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac
     9961 agactcttct tttctggaaa aggtaatgat tgactggaat cctagtttat gtaacagggc
    10021 tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt
    10081 cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag
    10141 gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac
    10201 tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag
    10261 tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc
    10321 aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc
    10381 atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc
    10441 agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga
    10501 tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag
    10561 cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga
    10621 atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca
    10681 aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt
    10741 tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt
    10801 tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag
    10861 ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta
    10921 ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc
    10981 aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat
    11041 tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg
    11101 aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat
    11161 tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt
    11221 cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa
    11281 aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa
    11341 ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa
    11401 gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt
    11461 aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat
    11521 tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc
    11581 caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag
    11641 atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc
    11701 cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt
    11761 gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta
    11821 tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga tctgcaacaa
    11881 cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt
    11941 gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt
    12001 tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac
    12061 ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat
    12121 ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc
    12181 acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc
    12241 cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa
    12301 aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg
    12361 cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg
    12421 ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt
    12481 tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc
    12541 aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga
    12601 tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga
    12661 ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc
    12721 ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg
    12781 gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag
    12841 acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt
    12901 tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga
    12961 atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg
    13021 gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg
    13081 gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact
    13141 gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc
    13201 tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc
    13261 tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt
    13321 ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag
    13381 ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct
    13441 tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga
    13501 aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg
    13561 atgttattat cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt
    13621 ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct
    13681 tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta
    13741 ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg
    13801 gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag
    13861 gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt
    13921 tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat
    13981 actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg
    14041 agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat
    14101 tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc
    14161 aaaggtactt accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt
    14221 tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt
    14281 cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat
    14341 ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt
    14401 aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat
    14461 tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca
    14521 aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga
    14581 cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa
    14641 gaatatggca cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa
    14701 agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt
    14761 gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat
    14821 cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa
    14881 taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa
    14941 tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga
    15001 catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta
    15061 caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat
    15121 gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct
    15181 aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc
    15241 gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa
    15301 aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga
    15361 aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa
    15421 actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag
    15481 ataaaaacta aattggtaga taaatt
//
LOCUS       DASJTM010000064        15356 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJTM010000064 DASJTM010000000
VERSION     DASJTM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.064"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..268
                     /locus_tag="U4B29_001961"
     CDS             2..268
                     /locus_tag="U4B29_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106903.1"
                     /translation="MNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVDENPT
                     NECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            287..1495
                     /locus_tag="U4B29_001962"
     CDS             287..1495
                     /locus_tag="U4B29_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2106904.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1643..2008)
                     /locus_tag="U4B29_001963"
     CDS             complement(1643..2008)
                     /locus_tag="U4B29_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN2106905.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2155..2928)
                     /locus_tag="U4B29_001964"
     CDS             complement(2155..2928)
                     /locus_tag="U4B29_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2106906.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3102..3962)
                     /locus_tag="U4B29_001965"
     CDS             complement(3102..3962)
                     /locus_tag="U4B29_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN2106907.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3962..5380)
                     /locus_tag="U4B29_001966"
     CDS             complement(3962..5380)
                     /locus_tag="U4B29_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2106908.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5437..5886)
                     /locus_tag="U4B29_001967"
     CDS             complement(5437..5886)
                     /locus_tag="U4B29_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2106909.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6331..6594)
                     /locus_tag="U4B29_001968"
     CDS             complement(6331..6594)
                     /locus_tag="U4B29_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN2106910.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6693..7988)
                     /gene="rho"
                     /locus_tag="U4B29_001969"
     CDS             complement(6693..7988)
                     /gene="rho"
                     /locus_tag="U4B29_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN2106911.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7985..9256)
                     /locus_tag="U4B29_001970"
     CDS             complement(7985..9256)
                     /locus_tag="U4B29_001970"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2106912.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9421..10290)
                     /locus_tag="U4B29_001971"
     CDS             complement(9421..10290)
                     /locus_tag="U4B29_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN2106913.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10582..12192)
                     /locus_tag="U4B29_001972"
     CDS             complement(10582..12192)
                     /locus_tag="U4B29_001972"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN2106914.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13385..14725)
                     /gene="glnA"
                     /locus_tag="U4B29_001973"
     CDS             complement(13385..14725)
                     /gene="glnA"
                     /locus_tag="U4B29_001973"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN2106915.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14753..15145)
                     /locus_tag="U4B29_001974"
     CDS             complement(14753..15145)
                     /locus_tag="U4B29_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2106916.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4360 a         3274 c         2636 g         5086 t
ORIGIN      
        1 gatgaacaca atcattaatc gattagaaac agcatatcaa acaagatttg atatgaaagc
       61 ttctttagtc tttttaaatg atgcgtatca aaactcgatc gaattgatca aagcagtgga
      121 tgagaatcca acaaacgaat gcgaagaatt tttaaatctg tttatgtcta caagagattt
      181 atttattcgc caacttgttg atcgctatcc atccaattat cacgatgtag aagttcaaat
      241 acaaaaatta aaagcatatt cagcttaagg ggaatgtata acatacatgg aaaagaaatt
      301 atttaataaa tattttattg gaatcacatt gatcaatttt attgtctacc tgatttatta
      361 tctgttgatg gtcatcatgg cagtcattgc gcaagacacc ttacacgcca gcttgagcca
      421 agcaggtttt gcttctggga tctatatcat cggaacattg ctggcacgct tattcatggg
      481 gaaacagtta gaactatttg gtcggaagtt taccttaaga ggcggcgcga tcttctatct
      541 cctttcaaca ttagcatatc tttatacgcc aactatcggg gcattgtatt tagttcgatt
      601 cttgaatgga ttcggctacg gtacagtttc tacagctaca aatgctatcg ttactgcata
      661 tatccctgca tctaaaaaag gggaaggcat caattattat ggattaagta ctagtttagc
      721 tgccgctgtt gggccatttt taggaatgat tttgctgaat atgactgatt ttcgtttcat
      781 tatctggttt tcaattgtac tggtcttctt tgttacgatt gcctgccttg tctttcctgt
      841 aaaaaatatt ccattatctg aagaacaaaa aatatctctt cgctcatgga atatcaatag
      901 ttttattgaa aagaaagcac ttttcattac aggaattgcc tttttgatgg gtctttctta
      961 ttcaagtgtt ttatcatttt tatcttctta tacaaaagta atccacttag ttgctgctgg
     1021 gtcatttttc ttcgtcgtat atgctttggt catcacattt acacgtccat tgactggaag
     1081 gattttcgat gtcaaaggag aacaatacgt gatgtatcct agctatttat tcttaacagc
     1141 aggattattt ttactgagtg taacgacgaa tagtgtgaca ttgcttatct ctggagcatt
     1201 agtcggccta ggttatggta cgtttatgtc aaatggacag gctgtttgtt taaaaatcgt
     1261 ccgcgaacat cgaatcagta ttgctttatc tacctacttt attggtttag acctaggctt
     1321 aggcgttggt ccttatttga tggggatgct aaaatctctt acttcattta gaggattgta
     1381 tgtgattgcc ggagtcattc cattgatatg tacagcactc tatcttgtta atggccgaaa
     1441 acatagtcat gaagcagagg gcgaattgat tgaatcattg aaaaatgaaa tttgatcttg
     1501 atgaatagat aaaaaaaacc gttcagatct gaaaataatc tgagcggttt tttgagtaaa
     1561 aatagtttat gattattctt aattaaacat ctatttattt ttaaagcttt catttatcga
     1621 taagaatagt ttaactgttg atttattcca ttttccgttc atctggtacg atgccttgac
     1681 ttagcaacca cttgtggaag aaccattctg ccacaccgat caatagagca ggaatcaata
     1741 ctttcatcca aatatcgact gtatcattca taaaatatag atagagccac aagataactg
     1801 cagctgaacc agcatctgcg atcgtcgctg cgatgttccc tactttacgt aagatcaaca
     1861 gatcccctag cacatagatc atgactacaa taattacccc aacaatcgtt gaatccataa
     1921 agctaacacc gtataaacct gtcagcacga cccacagaat cgcccagatc atgattgcct
     1981 tcactaccaa cgccttcaca tgtttcattt tctgcactcc tttctcctgg ataaaaaagt
     2041 aagattatct aacaagataa ccttttcatt attataccat cggctgagag aagcgagcaa
     2101 agataaggtc ctatctaaaa atgaggaagc cacttagaac attcacgtaa taatttatcc
     2161 gtacagacat tcgatatggt gttcctttac tttcgcatac tgttcttttg tcagttcttt
     2221 acttgtcacc agataatcga tttcatgaaa agcaatggct tgatgcatga atttatgtgc
     2281 aattttttgc tgtgtaacta aaacggcaag ccggtctgat tgttttctaa aagctttttt
     2341 gaccattgcc tctccttcat gagtttctgt tactccttgg ctgaaatcta gcccgcgaca
     2401 actcattatc gctaaatccg cataatatga caatgcatcg ttataggctt ttgaaccgtt
     2461 gattgttgct cgtttcggaa caattgcccc tcccaataca gatacttttg ccgttgtata
     2521 ttcactcaac atagcagctg tgacgatgcc attcgtaatg atctgcaaac catcaaatct
     2581 tgctaggttt ctgactaagt gtaaagaggt tgtactggaa tccaagaata agaccatatc
     2641 atctcgaatc aaagaagaag ctgtatctgc aatcttacgt ttttctgttt cttcttctaa
     2701 tttggtgatc ttcatcaggt aaggcacgtc aatttttcga ttgtcaataa gcatggcgcc
     2761 accgcgaact ctacggatca atccactttt ttccagaaaa acaaggtcac gtttaatcgt
     2821 ggcttcgcta taatttacta actcgatcaa atcggtgact cttgcaaatt ggttgttttt
     2881 tagataattc acaatctcct gctgtctatt ctccacttca ttcaacataa aatcacccca
     2941 tttactttat atagtaacag gaaaaaagag gttgtgacag aagtgattgg cttcgagaaa
     3001 taagaagaaa tttttcgaaa aaactacttc tgaacatcgt gtgtggttaa gagggtatga
     3061 caagactatt gtcataccct cttgaccaca catttttatt tttagtataa actagccttg
     3121 cctactgtgt tcaaaatttc cattttatgt ttgaccactt cttgtacctt tttattagct
     3181 gctggataaa ttgcattcgg ctcgtacatc gatggatttt cactcaatat ttctctcaaa
     3241 gcattgaaaa agacgctttt aagatctgag ctcaaattca ctttggcaac tccgtatttt
     3301 actgcttcgc tgacttctgc gtccggatta cctgaaccgc catgtaaaac aaatggaaga
     3361 tcgatttgtt ggttcaattc tttcagtaag tcgatattta attttggagt tctgtttttc
     3421 ggatacaagc catgcgctgt tccaattgct acagcaagtg tatcgattcc tgtttctttc
     3481 acaaaaatct tcgcttgatc aggatctgta taaatgattg tttcagcacc tccttcagca
     3541 gaaccattat tcccaatcgt tccaagctca gcctccactg agacatttac cgcatgagct
     3601 aacgcaacca cttttttcgt aatctgtaca ttgtcgtcga atgatttcaa agacgcatcg
     3661 atcatcacgg aagaatagcc gtttcgaatc gcccgtaaga catcttctat cgtaccgcca
     3721 tgatccaagt ggataacgat cggcagttta cttgtataag cataggctct tactgccgca
     3781 acaaactcat cacttaaata ggcgatttca tcaggatgaa tttctaagat tactggtgct
     3841 tctaatctct ctgcttcttc gatgattgct ttcatcatat catagctgca aatattgaag
     3901 gctggtactg caaattgatt ctctttagcc actttcaata actctttcat tgtatataac
     3961 attacgcttc cacctcattt gttagatttt catttttcgt ccacttgtct tgttttttct
     4021 ctttcactgt ttttttcaat agtccaagca taaaggctgt agtgactgta cctgatgcaa
     4081 ttgccaagac atacataggt aaattgttca taacatttgg aattagcatc acccaaatcc
     4141 ctccatgagg tgccattgaa gtaactccta ctgccatgga aatccctgca gcgactgctg
     4201 aacctgtcat caaactaggt atcacgcgga tcgggtcaga taccgcaaac ggtatagcac
     4261 cttctgtaat atacgaagcg cctaaaatcc agcatgattg acctgaagtc cgttcttctt
     4321 tggtaaattt atgtttgaat aataaagtgg ctaaagcaat acctagggga ggtgtcattc
     4381 ctgcagccat acaagcagca atcggtgcat ttactccagc tgacatcaaa ccgatcgaga
     4441 aagtcgaagt cgatttattg atcggcccac ccatatcagc tgccatcatg atcccgataa
     4501 gtaatccgaa caatagcccg gatgtatttc ccaagctatt cagccaattt gtcaaagcat
     4561 ccaacaccca tgtgatcggt tgttcaatca caaataccat caaaagccca ataattaaga
     4621 tcgataaaaa ggggacgaca atcagctgat aaatagcaga aaattctttt tttacgctaa
     4681 ttttatttgc cagaaaatca cagatatacc ctgctagcac gccaccaatc attccgccaa
     4741 gaaaacctga accacctgta gaggccagta atccggcaac aattcctggg gcaaaacctg
     4801 cttttccagc aatcgatgta gcaatccccg cgcctaacgc aggaaccatc atggcaaacg
     4861 ctgtatctcc tccgatcctt gaaaatgcag ctgctaacac attatagtct gctgagttgg
     4921 gatcagccgc tgtaatccca aaggcaaaac tcagtgcgat gatgattccg cctgcgatca
     4981 caaatggaag catgtaattc acaccattca tgaaatgatt atagattgtc ggctttttct
     5041 catttgcagc tggttctttt tcattagaca caacgaatac gccaatatct ctcaatgctt
     5101 cgtctattac tacttgcggt tctttgattg ctcggcttgt agagacacgc tttacttttt
     5161 tcccggcaaa tcggtccaat tcgatttctt tatctactgc aagaatgatt gcatctgcct
     5221 ctttgatttc ttgttcagat aactgatttt ctgtttttac accttgtgtt tcgaattttg
     5281 cttgatggtt tagatttctt gctgttactt ctaatttttc tgccgacata taagtatgag
     5341 caattccagt cgggcaagca gttacaccaa cgattttcat gattattttc ctcctttttc
     5401 ttcactatcg atactcggac aaagactcta ctattttcat tcttgactaa tctgtctgat
     5461 gatatctgct ggcgtagtgg cttcttttat tccctttcta aaatctgcat tcatcaatcg
     5521 tctggagagt gtgctgagaa tctctagatg catatctgct gatttttcag gaactacgat
     5581 ggcaaaaata tattcaattg gcatttcatc tatcccgcta tagtccgtta atttggctgt
     5641 atgaaaaaaa cacagtgctg gttttgtcac atatttgctt tttgcatggg gaatcccaaa
     5701 tccatcttct attcctgtgg agatctcctc ctctcgtttg aatagtgcct taacgacttt
     5761 ttgaacattt cttaccttat ttgttttttt caacacatcc cctattcgct caaataatga
     5821 tccttttgaa tctatcgatg gatcgaaaac aatcagttct tctgcgatca attgatcaaa
     5881 cctcataaaa aacctctccc ccacaaaaaa tgttaacgtt ttcttggaac tgaggtcagt
     5941 atagcatcgc tagctgtacc gttcaatatt atgaatgaac agaggaaaaa gacccatcat
     6001 ccgcgtgtat aagtcattaa aatgaacgat ctagctcaca aacaatcgca agtctataac
     6061 aaaacaaaaa gccttccttg aaaggaaagc ttagcttcta attttaggta aatggttttt
     6121 tacttcgaaa taacccctct tcatttcgtt aatataaggt gtttttatca agtggataat
     6181 taaaactata tactagtttt tcattgtttt gtgcattatg atcctacgac tttttcagca
     6241 tcaaacgaag ttttaaattg gcaaaaaaca taaaaaaaca gactatccct atcagatagt
     6301 ctgctaaacg ttgagttatc agcgttttta ttattccgca gcgtttgcgt ctttgagacc
     6361 gtattttttg ttgaaacggt ccacacgtcc gtctgcttgt gtgaattttt gacgtccagt
     6421 gtagaatgga tgtgagtcag aagttacttc gacacggatg actgggtatg tattaccgtc
     6481 ttcccattca actgtttctt gtgaagtttt tgttgaacct gacaagaatt tgaaaccagt
     6541 tgttgaatcc atgaatacta ctgggtggta atctggatgg atgttttctt tcatgatttt
     6601 ttcgctcctt tgccctgctc catttgctga accagagttg atttgtcgat ttacaacatt
     6661 aacatcttaa catgtttcag tcaaggttgc aattatcttt tcagatttct ggtcggattt
     6721 tttttaccaa aggagacatc ttgaaattct ttgaaaaatt gctgattgtt ttttgtttta
     6781 cgcaaaaagc ggatcagttg ttccgtatat tctaatgaat ctccagtcat attttttcga
     6841 agtttccaaa tttcttctaa ttcttcactt ggcatcaata attcttcttt tcgggtacct
     6901 gattttttga tatcgatggc tgggaagatc cgacgttcag ataattcacg tgaaagttgt
     6961 aattctagat ttccagttcc tttgaattct tcatagatca cgtcatccat acggcttcct
     7021 gtatcgacta aagcggtcgc taaaatcgtc aaactgccac cttcttcgat gtttcttgct
     7081 gccccgaaaa atttcttagg tttatacaat gcggctgggt caatcccgcc actcaatgta
     7141 cgtccgcttg gtggaacgac taagttatag gcccgagcaa gtctagtaat actatccatc
     7201 aagataacga catctcgttt atcttcgact aaacgcattg cgcgttccaa tactaattca
     7261 gaaactcgag tatggttttg cggctgcaga tcaaatgtag aagataccac gtcgcctttc
     7321 acgcttcgtt ctagatccgt tacttcttcc ggtcgttcat caatcagtag aacgattaat
     7381 tctacttccg gatagttttc ggtgatacca tttgcgattt cttttaatac agaagttttc
     7441 cctgcttttg gcggtgcgac gatcaatcca cgctgaccaa aaccgattgg agaaaagata
     7501 tcgatcattc gggtagatag tctttgcggt gttgtttcca gttgaagctg tctttgcgga
     7561 taaagaggtg ttaaggctgg aaaatgcgga cgttcttttg cttcttccgg atcttttcca
     7621 ttaacgcttt ccacgtgcat caaaccataa taacgttccg attctttagg tggacgggct
     7681 tttcctgcca ctttgtcccc atttctcaaa ctgaatcgtc tgatttgtga ggcagaaata
     7741 tagatatctt ctgcacttgg tccataattg attggccgta aaaaaccgta accatcttga
     7801 ccaacgatat ccaatattcc ttccatataa taaaatcctt gtttctctgc ttgtgcacga
     7861 atcacagcta gagataattc ctttttattc atcttactgt aataaggaat tttaaattct
     7921 ttggcataac tataaacttc ttttaacgtt ttgttctcta gttctgccat tgtcaaataa
     7981 tcgctcattc agctcccact tcctctatca tttcaattga tgcccctaag gctgttagtt
     8041 tctcgataat atgatcataa ccgcgtaaga tataatctac attgtaaatc gtcgttgtcc
     8101 cttctgccaa tagtccagca atcactaaac aagcacctgc ccgaagatca gaagccacga
     8161 cttctgtgcc gtgaagttta tttggaccat tcacgatgat catatttcct tccacggtaa
     8221 tatcagcgcc cattcgtgct agttccggca catggttcac acgtttcgca tagatcgtat
     8281 cgatgatttc tgaagtgcct gtagtcatca atagtaacgc agtaagtggc tgctgcagat
     8341 ctgtggcaaa tcctggataa ggataggtca tgatgtttgc gggttttaat tctttcgagg
     8401 ggtaaacttc gatttcatct tcgctgattt tcatatggac accaatttcc tgcaattttg
     8461 ctataaagct ttccaaatgt tcaaagatca catttttcac tttgatacca tttcctactg
     8521 cagctgctaa tgctagatag gttcctgctt cgatgcggtc aggaatcatg aaatgccgac
     8581 agccatgtaa tgtttcgact ccttcgatac ggatcacatc tgtacccgct cctcgaactt
     8641 ttgcccccat attattcagt aaagttgcca catctatgat ttccggttct cgtgcagcgt
     8701 tttcaattac tgtctgtcct tttgctttta cagcagctaa catcacattg atcgttgctc
     8761 cgactgaaac cacgtccatg aaaatccgat tgccacgcaa tcctttattt tctgtccgta
     8821 aatacatggc accatgttca tttgtcactt cagcacctag agcttcaaat cctttgacgt
     8881 gtaggtcaat cggtctaggg cctaaataac agccacctgg caggcctact accgcttcgc
     8941 caaatttacc aagcagtgtt cccataaaat aataagaagc acgcaaacta ttgatttttc
     9001 cgtgcggcat cggaatcgat acgacttctc gaggatcgat ttctagtatg ttgttggaaa
     9061 aatggacagt tgcgcccatg atctctaaaa tttcaattaa tgaatgaaca tcttgtatgt
     9121 ccggcacacc ttccaagatc actggggaat cagctaagat tgcagctgga atcaatgcga
     9181 ctgcactatt ttttgcaccg ctgatcgtta cttcaccagc taaaggtcgg tttcctttga
     9241 ttacgatttt tttcatctgt ttccaccttg tttattaatc ttttttcatg gatatgcttg
     9301 tattttatca tagataaaaa aaagaaacga gcatggtctg ccgttttaag taaaaaagaa
     9361 tggagagcga cttcaaaagt cgttctccat tcctatatat cgtatctgtt atgtggatgc
     9421 ttacgctttg ttagctgaac cgaaccagtc gatatgagat ttagcatctt caacgattgc
     9481 tgctttacct ggagctaata atttacgagg gtcgaatcct ttaccttctt tatctttacc
     9541 agcttcgata tatttacgag tagctgcagc aaatgataat tggaattcag tgttaacatt
     9601 tacttttgag atacccattg agatagcttt ttcgatttga tcttgaggaa tacctgatcc
     9661 gccatgtaat actaaaggca tgtctggacc tacagcatca gcaattgctt gtaagtggtc
     9721 aaaagctaat cctttccagt tttctggata ttgaccatgg atgttaccga taccgcaagc
     9781 taagtagtcg atacctgtag caaccatttg tttgcattct tcaatatcag ctaattcgcc
     9841 gttaccgatg attccgtctt cttctccgcc gattgaacca acttcacatt caactgagat
     9901 accttttgca tgtgcttttt caacaacgtc tttagctaat ttcaagtttt cttcaaatgg
     9961 tagatgtgaa ccatcgaaca ttactgaagt atagcctaat tcgatacatt ccaaagcagc
    10021 ttcgtaatca ccatggtcta agtggatagc aactggaaca gtgatgttca ttgcttcaac
    10081 taaatcagtg attaaatctt tacagatttt gtaaccaccc atgtatttag ctgcacccat
    10141 tgatgtttgg ataagtactg gtgcttgttt tgcttcagcc gcttcaagga ttgcttgagt
    10201 ccattccaag ttgtttgtgt tgaatccgcc gacagcgtaa ccgcctttac gagcagcttt
    10261 taaaaattca gctcctgata ctactggcat aataaaattc ctcctaagtt ttgtaaaact
    10321 aaatttgtta agacaattct taaccaacga ccctatttta ccaaacattt cggtagtttt
    10381 ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc
    10441 aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac aaataataac
    10501 aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt gctacttttc
    10561 tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg ctccgacaaa
    10621 tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt ggaattggca
    10681 tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt tatctggtga
    10741 gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt tattgaactc
    10801 atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt cagccgtctt
    10861 cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac ccaaattctc
    10921 gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg gattcgtttc
    10981 agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac aagccatctg
    11041 cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac gaatcgcttc
    11101 gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc catctgcgtc
    11161 tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc aatcgatctc
    11221 gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg agatatatgc
    11281 atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct tcaagttcaa
    11341 gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat ctagttttaa
    11401 atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga tggagtaaag
    11461 tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa attgtgccaa
    11521 tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa tattcggttg
    11581 gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt tcatttctcc
    11641 agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat ctgtaccgac
    11701 atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat cccctactgt
    11761 tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc gcatgatttt
    11821 ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca agtattcgcc
    11881 cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt tggagtattt
    11941 gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg tttcagcacc
    12001 atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg gatccacgtt
    12061 gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca ataagcgtcc
    12121 taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg tcacaaaaat
    12181 atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa gtctcagaca
    12241 ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg taaacttctg
    12301 accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa gtcaagtact
    12361 caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag aaattatttc
    12421 ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga tataaacata
    12481 gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa gtgaaataat
    12541 agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa agaactactt
    12601 tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt ttctttcacc
    12661 cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt tggcatatct
    12721 tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa gatgaccttt
    12781 ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa
    12841 taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt tctcctgcca
    12901 actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt gaaattattt
    12961 cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggta ttcaaaagcc
    13021 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
    13081 ttcttaatac tcgaaactaa acgtcttttt caaaacctct tggtaaacgg tgttcaaaag
    13141 cttgcatctg cggtaataag ttcagccaat tcaaaaacaa gaaaagctgt ttttgcttgg
    13201 ctattcttat tacttgttgc agggcagctt tttcacaacc tctccactaa acggtgttca
    13261 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    13321 tagtttctct aatactcagg tcaaaacgct gatttcacag cctctaattc acggtattcc
    13381 tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt tgtctgaatg
    13441 ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt cctaatgcgt
    13501 caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt gaaggtaagt
    13561 cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta cggtcaacag
    13621 ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac aatactgcca
    13681 ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt gtggataaac
    13741 cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat gcgacataaa
    13801 caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga ttgcaaacgg
    13861 ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct gtttcactta
    13921 gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac aaagacatat
    13981 tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa gtcgcgtgaa
    14041 gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata ttatcacaag
    14101 catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct acttcatgat
    14161 gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca cggcggcaat
    14221 tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc aaggtcgttg
    14281 tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt cctaaattga
    14341 aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc ccgcgaggat
    14401 cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga gcaacttttc
    14461 catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac aaatacatat
    14521 cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc attttatttt
    14581 ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta cctaagatgt
    14641 ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt ttgatttctt
    14701 cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt attcagtcaa
    14761 agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac ccaccttgcg
    14821 ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc ttcttgtttt
    14881 ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca gccatattca
    14941 atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc agcgaataca
    15001 tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca taatagcgaa
    15061 tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat acggacatag
    15121 atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag ttggctaaag
    15181 tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac atgagatctc
    15241 tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt tgattttcgt
    15301 accagatacc ttttattttt tattatactg tactatcctt tattagtgtt tttatt
//
LOCUS       DASJTM010000065        15271 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJTM010000065 DASJTM010000000
VERSION     DASJTM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.065"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="U4B29_001975"
     CDS             288..695
                     /locus_tag="U4B29_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106917.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="U4B29_001976"
     CDS             709..1110
                     /locus_tag="U4B29_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106918.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="U4B29_001977"
     CDS             1112..1483
                     /locus_tag="U4B29_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106919.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1824)
                     /locus_tag="U4B29_001978"
     CDS             complement(1519..1824)
                     /locus_tag="U4B29_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106920.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2351..2423)
                     /locus_tag="U4B29_001979"
     tRNA            complement(2351..2423)
                     /locus_tag="U4B29_001979"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Lys,seq:ctt)
     gene            2804..3373
                     /locus_tag="U4B29_001980"
     CDS             2804..3373
                     /locus_tag="U4B29_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN2106921.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3360..4220
                     /locus_tag="U4B29_001981"
     CDS             3360..4220
                     /locus_tag="U4B29_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2106922.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4410..5114
                     /locus_tag="U4B29_001982"
     CDS             4410..5114
                     /locus_tag="U4B29_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2106923.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5119..6954
                     /gene="walK"
                     /locus_tag="U4B29_001983"
     CDS             5119..6954
                     /gene="walK"
                     /locus_tag="U4B29_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN2106924.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6951..8267
                     /locus_tag="U4B29_001984"
     CDS             6951..8267
                     /locus_tag="U4B29_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106925.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8268..9137
                     /locus_tag="U4B29_001985"
     CDS             8268..9137
                     /locus_tag="U4B29_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN2106926.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9192..10001
                     /locus_tag="U4B29_001986"
     CDS             9192..10001
                     /locus_tag="U4B29_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2106927.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10104..10733)
                     /locus_tag="U4B29_001987"
     CDS             complement(10104..10733)
                     /locus_tag="U4B29_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN2106928.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10903..12195
                     /locus_tag="U4B29_001988"
     CDS             10903..12195
                     /locus_tag="U4B29_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2106929.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12271..12933)
                     /locus_tag="U4B29_001989"
     CDS             complement(12271..12933)
                     /locus_tag="U4B29_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2106930.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13158..13442
                     /gene="groES"
                     /locus_tag="U4B29_001990"
     CDS             13158..13442
                     /gene="groES"
                     /locus_tag="U4B29_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN2106931.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13493..15118
                     /gene="groL"
                     /locus_tag="U4B29_001991"
     CDS             13493..15118
                     /gene="groL"
                     /locus_tag="U4B29_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN2106932.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5189 a         2553 c         3191 g         4338 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcaca tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatc
     1741 ccacacctta taaaaatctt tgacaaattt ttctgatgga tcgtatttaa accaaagcag
     1801 atgttcctct agctcgattg tcatgttttt cacctcaacg aaagtatacg aactaatgtt
     1861 tgttttgtaa aggataaaaa tagggtagca accggtaccc aaactagtac ccatttatca
     1921 aaaacgacga aatttatgta aatagaaaaa taagaataaa cattgatgta tcaacattta
     1981 tatatcttta gaacaaatga aaagggtggt tctcataaga aaaacatgtt cgcacttcta
     2041 ggcaaaccag gatttgaaga ccttgctaaa gaattgaacg aacgcctgta aaataaggat
     2101 ttgtcgctag atttaaaaaa tcagtgatag aaacatgttt gctttaaaat gaaactagtg
     2161 atagttttag tgatagtgaa cttaaaaaaa gcactcaatt tgagtgcttt tttattgtta
     2221 ttaaaaatta aaaaactaaa atacataccc gaatttcata cccgaaatat aaaaaatgtc
     2281 atcacgtaca tctttttcga aactaccgaa gtagccaaag atgtactgat gacgtgggtt
     2341 tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc gctgattaag agtcagctgc
     2401 tctaccaact gagctaacgg cccgtacaaa aagtacttaa taatagtagc actaagaatt
     2461 ttaaaatgca agactacatt taaagaaaat cagaaaaatc tttacttcct tataggaaat
     2521 cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa acaaagttct
     2581 tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc cgtctccccg
     2641 gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg ttttttgttt
     2701 tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat gtttatcata
     2761 agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca
     2821 gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact gggaagaact
     2881 ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt atctttcacc
     2941 tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca attacgttaa
     3001 actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg tagcattttt
     3061 acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag aagggatatt
     3121 gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg atgaacaaga
     3181 agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc aaaaattcga
     3241 ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt ttgcaatgaa
     3301 acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa
     3361 tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgttcca gaagaattga
     3421 taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat caggtatata
     3481 cagataaaat tgacattacc cctgaagaga tctatcaacg tttgccagag gaaatccctg
     3541 gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg
     3601 gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca tataatgtgg
     3661 ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat acgaaaaata
     3721 ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa gaaggagtag
     3781 aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa gttttcttta
     3841 atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt gcttctattg
     3901 taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg atttattaca
     3961 cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag
     4021 aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat gcgaaagaag
     4081 aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac caaatctact
     4141 ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg ggtgtcggtg
     4201 tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc
     4261 cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca ttttaatttt
     4321 ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa tgagaaggta
     4381 tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc gatgatgaga
     4441 agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgac gtttatactg
     4501 catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat ttgattttac
     4561 ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta cgcaaaactt
     4621 atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa gtcctaggct
     4681 tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa ttagttgcac
     4741 gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac
     4801 ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg gtgacgaaac
     4861 gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac ttggcgaagc
     4921 accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt tatgattatt
     4981 ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata
     5041 atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc
     5101 cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt caattttaag
     5161 atcgctatta cctttattct gattttattg atctcgatcg aaattattgg tgcctacttt
     5221 atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa tcaaacggtg
     5281 gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc agatgatgaa
     5341 gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga tatcatcgaa
     5401 atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc
     5461 gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa acgttatgtt
     5521 gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct gtccccgacc
     5581 ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa atattcggaa
     5641 atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt tatttcaatg
     5701 atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat gagagaacaa
     5761 gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg acaagatgaa
     5821 cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga agctcaagat
     5881 acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac agacggcgtc
     5941 attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt ttccttgcta
     6001 gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga tatcgaagaa
     6061 gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga ccgttcaacc
     6121 agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat ccgtcgcgaa
     6181 tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca
     6241 gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac accgttgaca
     6301 agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga ggaagtagca
     6361 ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat gatcaatgac
     6421 ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga atatgtcaat
     6481 ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg aaatcaggaa
     6541 aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga agtagataca
     6601 gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta ttcaccagat
     6661 gggggaacga ttacttgtcg cttattagaa acgcataata acgtgatttt aagtatcacc
     6721 gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg gttctatcga
     6781 gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc tatttctaga
     6841 gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg
     6901 actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga atgagaatct
     6961 ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata tacttctctt
     7021 atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc
     7081 aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta
     7141 catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg taccgattgc
     7201 aattgatcat cgataaagca agatttggaa agctttctga agtagtttca ggagacaaat
     7261 cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat aatgcgcctt
     7321 ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg aacttggcaa
     7381 aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat aaaaaaatcc
     7441 gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg aattggacag
     7501 atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca acagaaccat
     7561 cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaagaag tatagttata
     7621 tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa ccagatagcg
     7681 tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa acaatgtcta
     7741 tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa aaagaaaatg
     7801 ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct ttagggaacc
     7861 tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg gaaggcttcc
     7921 cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt gaaacgaatg
     7981 gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg ataccttcag
     8041 acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa gctaagggag
     8101 cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa
     8161 cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat aactggtatt
     8221 cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg gatttcaaaa
     8281 aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt
     8341 atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact gaatcaatcc
     8401 aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct gagaacaagc
     8461 aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg aattatcgca
     8521 aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat gttttgacga
     8581 aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa attagtagtt
     8641 ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat tatgtgtaca
     8701 cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc caagagtatg
     8761 aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa aaaaacgata
     8821 atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag cagctgcgcg
     8881 aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc aataacaaaa
     8941 taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc
     9001 gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc gataaagtgg
     9061 tccaaattga gagcgtcaac gctttcagca atacgatcgt gacaaacaat actgtaccaa
     9121 aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt aagaagggaa
     9181 gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta gcaagtggaa
     9241 gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac
     9301 ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg gaccaattag
     9361 atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt gtcttggctc
     9421 gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg gaaccactga
     9481 ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc agaacattcg
     9541 gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg caattttatc
     9601 gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat tgcagtgatt
     9661 atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat catgatcttg
     9721 agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta ggagatagag
     9781 gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt aatcggacca
     9841 aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt gctcatttaa
     9901 caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt gaaatctatg
     9961 atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa tcaataaaaa
    10021 tataaaaagc agaagataaa atcaatcagg gttggtttta tcttctattt ttattttttt
    10081 agcatattaa aaaaataacc gtattatcga ctgactacac tgatgtgctg cttttcatgt
    10141 ttctcatcca gtatctcatc tactaatgca atcgcgtaat ctgccatgct gatcgtactg
    10201 tcgctagcag cgtttctttg taaataatca tcacttaaca aataagtacc tgtacgtttg
    10261 ccatgtggat caaataaaga agctggactg atataagtcc aatgtagatt tttagattgt
    10321 ttcaatttta acaatccttc gtacatattg aaagctgttg gatagaaggg tgcattttta
    10381 ggtacttggt caatcatacg agtcgtcttt tctgtatcta agaataacga agaggcaccc
    10441 ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct caatcaaatg ggacaaagaa
    10501 gtttgatgca tttcttcttt tcctttggga gcgttgaaag catcaacgac aacatcaaaa
    10561 gatgcaagat cagtagttgt caactcaaat aaatcctttt gtataaacgg aacatctgat
    10621 ggcaattttt ctggatgacg cacgattgcc gtcacgtcta aatctctgga caatgcttcg
    10681 tctaaaataa atgaacctgc atggccagta gctccaatga gtgcaatttt cataaaaaac
    10741 ctcctttttt attcctgtcc attgtaagca gttgctgaat attcgtcaaa gaatccgatt
    10801 tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat cattgggtat agttaaatta
    10861 agaaaactga ttgataaaaa tagaagttga aggagataag aaatgaaaga gtggttagat
    10921 cgtgcctttt taccaatgat aacatctgtt gtgaatctta gacccttaac tgctttgcgt
    10981 aatggaatga cttacacgat cccaataatt attgttggct cgctgttttt cttatttgct
    11041 cattttcctg tggattacat cgctcagtgg atagacaaaa caggattttc tatgtatttt
    11101 acacaagtat accaggctac atttgatgtg atagcctttt ggatcgtttt tgccattgca
    11161 tacgcttatg caaaagaaga agacatcgat cgcttaccag tcggcataac agccttgtct
    11221 gcttatctac tgcttattca gccactaaat actcagatag atactgtctg gacaggtgct
    11281 gaagggatac taggagcagt tacaactggg ctcatcatcg gatggggcta tgcacgttta
    11341 gtaagacttg cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt ttggcagtct
    11401 tttacttcat tgattccaat cattgttata gtcactttta cgcttatcgg ttcagcggtt
    11461 tttcaacatt tgttcagaat gactgttgtt caaggagtat ggacatatgt ccagcttccc
    11521 attcaatcgt tcatagacac gttgtttggt gtgcttttat taggtttttt tgttccgttt
    11581 ttatggttat ttggtcttca tggatctgcg atggtcaatg ggttggttag tcccgtcttg
    11641 caagcgaatt cattagcaaa tgcagaaatc ttagcctcag gacaagagtt gacggtggca
    11701 aatggcggac atatcgttac tcagcaattt ctcgatcagt tcatgacggt tacgggtgct
    11761 ggattgacgt taggggctgt attttttatg acggtttttg cgaaatcgaa gaaatacaga
    11821 gaattaggaa atcttgctct tttaccagcc tttttcaaca tcaatgaatc aattattttt
    11881 tccacaccga tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc accggtcgtt
    11941 tcaggattga ttacctattc cgctttatat tttggattcg ttcctttatt tacagcagtc
    12001 caagtccctt ggacaacacc gccgatcctc tcgggtttct tagtaggcgg agtgcctgca
    12061 gcgattttac aaggtatcgt gttgtcaatt agttttttca tttatttccc atttttgaaa
    12121 aaaatagatt ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg agcagcagaa
    12181 aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt agattgggaa aatcaccaat
    12241 ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga cagctaattg
    12301 aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc agatccgacc
    12361 agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta gcagatgacc
    12421 atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc aaaaatttga
    12481 atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca tgatgggtcc
    12541 agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga tgatattttg
    12601 tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga cagcgatgct
    12661 ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct ttggagcaga
    12721 agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat aaaaaatagc
    12781 taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt cagacgtaga
    12841 ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac catagcaaaa
    12901 aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa gggataacaa
    12961 gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca ataagcagct
    13021 gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg agaatgagta
    13081 ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta aaaaaagagt
    13141 tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc gaagtcgcaa
    13201 aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa gagaaacccc
    13261 aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt gaaaaagttc
    13321 ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt acagaagtaa
    13381 aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg attgttgagt
    13441 aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa tcatggcaaa
    13501 agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag ataaattagc
    13561 agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag aaaaatctta
    13621 cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg aattagaaga
    13681 tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa caaatgatat
    13741 tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc gcgaaggttt
    13801 aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg aattggcaac
    13861 aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa aagaagccat
    13921 tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga ttgctgatgc
    13981 aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag ggatcgaaac
    14041 agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac aatatatggt
    14101 aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga tcacagacaa
    14161 aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc aacaatcacg
    14221 tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc ttgtattgaa
    14281 caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg gcgatcgtcg
    14341 taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta ctgacgatct
    14401 aggtttagaa ttgaaagatg caacaatcga aaacttagga aatgcttcta aagttgttgt
    14461 agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa tcgaagctcg
    14521 tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc gcgaaaaatt
    14581 acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg gtgcagctac
    14641 tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg ctacacgtgc
    14701 cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg ttatcagcaa
    14761 agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg ttgttcgtgc
    14821 tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat cagtgattgt
    14881 tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg gagaatgggt
    14941 aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg ccttgcaaaa
    15001 tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag ataaaccaga
    15061 accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta tgatgtaaga
    15121 aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa agaggctgga
    15181 acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt ttcaaatttt
    15241 tgtgaatttc agcttatttc cgaaggagtt g
//
LOCUS       DASJTM010000066        15255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJTM010000066 DASJTM010000000
VERSION     DASJTM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.066"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="U4B29_001992"
     CDS             248..1021
                     /locus_tag="U4B29_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2106933.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="U4B29_001993"
     CDS             1018..1785
                     /locus_tag="U4B29_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2106934.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="U4B29_001994"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="U4B29_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN2106935.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="U4B29_001995"
     CDS             3750..4070
                     /locus_tag="U4B29_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN2106936.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="U4B29_001996"
     CDS             4070..4420
                     /locus_tag="U4B29_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2106937.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="U4B29_001997"
     CDS             4587..5525
                     /locus_tag="U4B29_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN2106938.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="U4B29_001998"
     CDS             5540..6370
                     /locus_tag="U4B29_001998"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN2106939.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="U4B29_001999"
     CDS             6414..7439
                     /locus_tag="U4B29_001999"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2106940.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="U4B29_002000"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="U4B29_002000"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2106941.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="U4B29_002001"
     CDS             8517..8957
                     /locus_tag="U4B29_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN2106942.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="U4B29_002002"
     CDS             8960..9562
                     /locus_tag="U4B29_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2106943.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="U4B29_002003"
     CDS             complement(9625..10728)
                     /locus_tag="U4B29_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2106944.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="U4B29_002004"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="U4B29_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN2106945.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="U4B29_002005"
     CDS             12294..12689
                     /locus_tag="U4B29_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106946.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="U4B29_002006"
     CDS             12689..12940
                     /locus_tag="U4B29_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106947.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="U4B29_002007"
     CDS             12983..13378
                     /locus_tag="U4B29_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106948.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="U4B29_002008"
     CDS             13375..14631
                     /locus_tag="U4B29_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEN2106949.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="U4B29_002009"
     CDS             14643..14966
                     /locus_tag="U4B29_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106950.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="U4B29_002010"
     CDS             14959..15225
                     /locus_tag="U4B29_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106951.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DASJTM010000067        15158 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJTM010000067 DASJTM010000000
VERSION     DASJTM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.067"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="U4B29_002011"
     CDS             complement(164..388)
                     /locus_tag="U4B29_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN2106952.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="U4B29_002012"
     CDS             complement(586..2166)
                     /locus_tag="U4B29_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN2106953.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="U4B29_002013"
     CDS             complement(2567..3943)
                     /locus_tag="U4B29_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2106954.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="U4B29_002014"
     CDS             complement(4071..5189)
                     /locus_tag="U4B29_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2106955.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="U4B29_002015"
     CDS             5331..5765
                     /locus_tag="U4B29_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2106956.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="U4B29_002016"
     CDS             complement(5843..6376)
                     /locus_tag="U4B29_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN2106957.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U4B29_002017"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U4B29_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN2106958.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U4B29_002018"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U4B29_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN2106959.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="U4B29_002019"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="U4B29_002019"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2106960.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="U4B29_002020"
     CDS             complement(10101..10679)
                     /locus_tag="U4B29_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN2106961.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="U4B29_002021"
     CDS             11001..11471
                     /locus_tag="U4B29_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN2106962.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="U4B29_002022"
     CDS             complement(11579..12805)
                     /locus_tag="U4B29_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2106963.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="U4B29_002023"
     CDS             complement(13559..13759)
                     /locus_tag="U4B29_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2106964.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="U4B29_002024"
     CDS             complement(13893..14501)
                     /locus_tag="U4B29_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN2106965.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="U4B29_002025"
     CDS             complement(14586..15056)
                     /locus_tag="U4B29_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN2106966.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         4494 a         3059 c         2488 g         5117 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgccttt
//
LOCUS       DASJTM010000068        14549 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJTM010000068 DASJTM010000000
VERSION     DASJTM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.068"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..816
                     /locus_tag="U4B29_002026"
     CDS             4..816
                     /locus_tag="U4B29_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN2106967.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="U4B29_002027"
     CDS             813..1613
                     /locus_tag="U4B29_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN2106968.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="U4B29_002028"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="U4B29_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN2106969.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="U4B29_002029"
     CDS             3110..4819
                     /locus_tag="U4B29_002029"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN2106970.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="U4B29_002030"
     CDS             5031..9389
                     /locus_tag="U4B29_002030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN2106971.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="U4B29_002031"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="U4B29_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN2106972.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="U4B29_002032"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="U4B29_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN2106973.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="U4B29_002033"
     CDS             11227..11520
                     /locus_tag="U4B29_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN2106974.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="U4B29_002034"
     CDS             11517..11828
                     /locus_tag="U4B29_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN2106975.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="U4B29_002035"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="U4B29_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN2106976.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="U4B29_002036"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="U4B29_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN2106977.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DASJTM010000069        14528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJTM010000069 DASJTM010000000
VERSION     DASJTM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.069"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(198..2273)
                     /locus_tag="U4B29_002037"
     CDS             complement(198..2273)
                     /locus_tag="U4B29_002037"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN2106978.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2275..3192)
                     /gene="glyQ"
                     /locus_tag="U4B29_002038"
     CDS             complement(2275..3192)
                     /gene="glyQ"
                     /locus_tag="U4B29_002038"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN2106979.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3578..4396)
                     /gene="recO"
                     /locus_tag="U4B29_002039"
     CDS             complement(3578..4396)
                     /gene="recO"
                     /locus_tag="U4B29_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN2106980.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4539..5438)
                     /gene="era"
                     /locus_tag="U4B29_002040"
     CDS             complement(4539..5438)
                     /gene="era"
                     /locus_tag="U4B29_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN2106981.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5453..5848)
                     /locus_tag="U4B29_002041"
     CDS             complement(5453..5848)
                     /locus_tag="U4B29_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN2106982.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5832..6308)
                     /gene="ybeY"
                     /locus_tag="U4B29_002042"
     CDS             complement(5832..6308)
                     /gene="ybeY"
                     /locus_tag="U4B29_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN2106983.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6337..8529)
                     /locus_tag="U4B29_002043"
     CDS             complement(6337..8529)
                     /locus_tag="U4B29_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN2106984.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8553..9524)
                     /locus_tag="U4B29_002044"
     CDS             complement(8553..9524)
                     /locus_tag="U4B29_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN2106985.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10229..11608
                     /gene="brnQ"
                     /locus_tag="U4B29_002045"
     CDS             10229..11608
                     /gene="brnQ"
                     /locus_tag="U4B29_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2106986.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11890..13230
                     /locus_tag="U4B29_002046"
     CDS             11890..13230
                     /locus_tag="U4B29_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2106987.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            13387..>14528
                     /locus_tag="U4B29_002047"
     CDS             13387..>14528
                     /locus_tag="U4B29_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2106988.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQI"
BASE COUNT         4067 a         3078 c         2685 g         4698 t
ORIGIN      
        1 aagtaaataa aacagtaaca agattataat taatcgtttt catttttatt tcctcacttt
       61 tcatgagcgt tttgaaaatt cgaacaaaga ataaaataac aaaaattatt aaaatagaat
      121 taataacagc tatttataac aaaaaaaaca gagacacagc acgtttcgtg catgctctct
      181 gtttttcaaa caaaagacta ttttgtgatg atttgatcca agcttgctaa tgacaaagcc
      241 atggcagcta tttgacttag ttgtgttagt cggttcttac gtactgctgg ttcatcagcc
      301 atgaccatcg tttgatcaaa ataattttcg atcagtggac gaagagcgac aagctggtcg
      361 tagttttcgt ggatcgtttg gtcagcaaaa cttgctttca ttgcttctac tgctttgtgt
      421 aattctttct ctgcgtcgtt ttcgaacagc tcagggtcta cttctgattg tttttcaagc
      481 tgcgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccattgaagg tttgaagtct
      541 tcatctgcaa gatgttgttt gaacaattgt gctgctgcaa atacttttgt cagatctttt
      601 tgttccgcat tcaaaacagc atcgatgaca tcatgacgaa tattcttcgt tgtcaataat
      661 tggcgcagac ggcctttgat aaagtcgatc acttgttgtt gcccttctga taaaccaatg
      721 ccgaaacgat ccgtatcttt actgataaca tcgctaatcg tttcttgcag aacagacaaa
      781 ggaaaggccc attttttcga ttcgatgatc cgaacgacac cgtatgcttg gcgacgtaag
      841 gcataaggat cgtttgaccc gcttggaatc atcccaactg caaagaaaga aaagatgcta
      901 tctaatttat ctgcaatcgc taaaacagaa cctactgtag actgaggtag ctctccttca
      961 ctggatatag gcatgtaatg ctcacgaatc gctgtcgcta cagcaggttt ctcgcctttc
     1021 agaagagcat atttttcgcc catgatccct tgtagttcag gaaattcccc taccatgttg
     1081 gttactagat caaatttata gatttcagca gctcgttgca aatccatcaa ttcttcctca
     1141 gatagtccta cttgttcgcc aatgatacgg gcaatgactt gtacccgttg cattttctca
     1201 tagatactac cgattttttc atggaaagta acagttttta gtttttcaac gtattggtcg
     1261 attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg agccgtcaat
     1321 actttttgat tgcctcgaac cacatcttcc aattttaccc gatcaccatt acgagcagaa
     1381 ataaaatgag gaagcagcat accttgatca ttccgtacct cgaaataacg ttgatgttct
     1441 ttcatagaag tgactagtac ttcttcagga acagaaagat atttttcttc gaagtcgcca
     1501 gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc ttcgtctaga
     1561 tcaataaccc agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt
     1621 cttttttgcg gagacgcaat gacgaattgt tcattcaatt tttcttcata ttcttttgca
     1681 tgcgcaaatg tcacatcttc gcctaagaaa cgatgacctc gagaggctct gcctgttttg
     1741 acatccaaga tttcgaatgg gattacttcg tcatctaaca atgccacgat ccagtgaata
     1801 gggcggatat attcaaaatc gtattctgcc caatgcatcg ttacagggaa agtcaaactt
     1861 ttgatcaccc ctgctaagcc tgtcaatact tcttttgctt ccttaccatg agtgtatttc
     1921 gttacataga catattccac accttttaat tctctaaaag tgatagcgtc tgtcgtcaga
     1981 ccttgtccgc ggacaaatcc ttccgctgct ttcgtccagt ttccatctgc gtccaaagca
     2041 atttttttgg ctggtccttt gacttcttct tcactgtccg cttgtcgttc tggaatattt
     2101 gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat
     2161 tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgaca
     2221 acatgggcag gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc
     2281 agcctccttc acggtttctg gatttgctgt gtttttattc aacaaaggga atcctaattt
     2341 ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga tgcgagaaag
     2401 ataaccggca cgctctgtta cggatactgc accgcgggcg tctaataagt tgaatgtatg
     2461 gctacatttc aagatataat catacgccgg atgaacaaga ttttcatcga tacagcgttt
     2521 agcttctttt tcaaaacgat caaagttgct tagtagcaag tcttggtcgc ttacttcaaa
     2581 agaatatttg gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc
     2641 agcccattct aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa
     2701 tccataagtg atctcagaag ttactggttt acaaggcagt ccaccaactt gctggaagta
     2761 cgtgaattgt gtgatttcca ttccgtcaag ccatacttcc caacccacac cggcacagcc
     2821 catggatggg ttctcccagt tgtcttcaac aaaacggatg tcgtgttcta atggatcgat
     2881 tcctaaaagg cgcaagcttt ctaaataaag ctcttgaata ttctcaggtg aaggtttcat
     2941 caccacttgg aattgatgat gctgatacaa acggtttggg ttttcaccat aacgtccatc
     3001 agctggtcgg cgagatggct ctacgtaagc agcattccat ggttctggtc cgattgcccg
     3061 taaaaaggta tatgggctca tcgtacctgc tcctttttct gtgtcataag cttgcatcag
     3121 catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa
     3181 ttttttactc atctgttttg cccctttcaa ttttaaagcg ttgctgtcaa ataagaatac
     3241 aggaaagaaa acgacaaaaa atccctatgt ctgattacag acatagggac gaataattcg
     3301 cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc
     3361 ctcttcaaaa atcgtttggg tccggctccc actctcccga actcgctaaa ccacgtgttt
     3421 tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttcttgtttc tataatagat
     3481 cctatcactg aacgatctat ttaatctatg gttaataata gacggaactc cctgtgtttg
     3541 tcaaatggtt ttgatttttt gttgtatgtt ttatttacta ttcttctttt tcaggtgtct
     3601 ctttttcttt ttcagacatg tctttctctg acgtctttaa tacgtgtgcc caagacttca
     3661 tttgattgat gaactttttg cttttcaaat ggataccgac atattcatca tacagctggt
     3721 cgatgagttc acggattgct tgtttggttt cttctttcag atggattcca ctaattttat
     3781 cataagaaat agctgaaaac atccgaacga aataaacagc tcgaggatct gcatgataac
     3841 ggtgatcgtc catcgaccaa tgtctttcgc atatcacacc accatatttg gaagaatagt
     3901 caaattttcc ttttgtttcc ccgcatacga cgcagtgtgt ccagattggt gcaattccga
     3961 agcgttgcag tatttggact tcaaaaatat tcgtgatgat ttctgcatcg tctccttggt
     4021 ccatccgttg cagtgcttgt tgcgtaaact ggaacaaatg cgggtcgtac tgatgatctt
     4081 cgattgccgc atctactaga ttcaataaat atgtgccata gccgctcaaa aagatgtctt
     4141 cttggatgtg tcgaaacgca tggacatctt tgacactgct taaaaatgac aaaccttcac
     4201 ttcgaaaatt cccgatataa actgcttcag taaaagggag caaagccgga gctaagggct
     4261 gattttgccg atggatctct ttgatataaa acatcatttt gccagaagat tctgtaaaga
     4321 ttttgactaa tttatctttt tctttgtaat tacggctaga taaaatcaag ccttttgatt
     4381 ctgcaagctg gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg
     4441 atccagatca tttatgttgc gatcttccgc agcttttctc aacacgcgga tttttattca
     4501 gtttatttct atacctaatt tacaagaagc tcagaatatc agtattcgtc ttttcggtag
     4561 ccgaagtctt gcagatagac ttttttgtct cgccagtctt tttgaacttt gacccaaagt
     4621 tctagaaaga ctttgtctcc taaaagattt tcgatatctt ttcttgcttt cgttcctact
     4681 tgcttcagca tttttccgcc tttgccgata atgatccctt tttggctatc tctttcgaca
     4741 ataatcgtag cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg
     4801 actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat
     4861 tcagaaacga tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg
     4921 ccctctggca tttcactgac taatacatcc atcaaacgtt cgacgttgtt tccttctgta
     4981 gcagagattg gaatcacttg tgcaaaaggc atctgcgttg aataatcttc gatgattccc
     5041 aacaactcgt ctggatggat tttgtcgatt ttattgataa tcagataaac aggagaatgc
     5101 acgtttttta gacgttcaat aataaaatcg tctccccgac cgcgtttttg atcagcacta
     5161 atcataaata agaccgcgtc cacttctttc aacgcactgt aagctgcttc taccataaaa
     5221 tctcctaaac gatgctttgg tttatggatt cctggtgtat caataaaaat caactgagct
     5281 tctggtgtag tgtatactcc ttggatttta tttctagttg tctgtgcctt gtcactcatg
     5341 atggcaattt tttgacccac gattcgattc aataatgtgg atttccctac atttgggcgg
     5401 ccgacgattg cgacaaaccc tgatttatgt tctggcatat atgttctcct ttttaattaa
     5461 acaacaaatg atatatttta gggacaaata aaatcaaccc aacgatgacg gtaaaacaag
     5521 cggtcactaa aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat
     5581 gaaaatccgt aaacatgtcc acgacatttt caaaaacagt attgatgatc tctactatcc
     5641 atacgagaaa aacagctaaa agcagccata accattctat ccgatcaagt tggaaaatca
     5701 cgcccataaa caaagcgagg attccgaacg caacatgttt cctcatgttg cgttcttcct
     5761 caaaaacagt tttgactcct tggacggcaa attccacgga catcaggaaa tgtttgtttt
     5821 tctctacgtt tttatctttt aagtccatag gcatctaaga tctctttctg taaaccaaac
     5881 atttctttct catcctctgg tgtcatatgg tcatacccgt tgatatgcaa gaatccatga
     5941 accgctaaaa agccaagttc ccgatcatac gaatggccgt aagcttctgc ttgctctgca
     6001 gcacgttcag tagagatcat gatgtctccc agtacacgag gcaatggatt ctctccatct
     6061 tcaaaaatca ctgggatttc gtcttcgccc tcttcttcca aggcaaaact gatgacgtct
     6121 gtcggtgcat cttttccacg gtaatcacga ttgattactt ggattgctgc attgtccatg
     6181 aaagtaacag acatttctgt ttcttctggc aactctaaat atgatgctgc aaattgcagc
     6241 aagtcgtcga tttctttcat tttctcttct gagacttggt ttgtttcatc catgaaggta
     6301 atatccatta tacatcgctt ctttctattc ttcttgctat tcttcttgtt cttcttttat
     6361 cttctcaact tccgatttca ttttaggata tttgatccga gaatggaagg tactgcttaa
     6421 accgctgatc aatgattttt ctactttacg gatttctttt aacgtcaatc cactgtcgtc
     6481 caattgtcca tcggaaatac gctcttcaat aagattatgg acaaaacttt cgattttttc
     6541 gctcgttgga tggtccattg cccgtactgc cgcttcacaa ctgtcagcta tattgacgat
     6601 tcctgcttca cggctttgag gttttggtcc aggataacgg aattgctctt ccgtaacttc
     6661 cgggttgcgt tcctttgcct taacgtagaa ataacgcatc agcgtggttc catgatgctg
     6721 tctacaaata tcgatcacca tttgcggcat attataatct tccaatattt tggcaccatc
     6781 tgtcacatgt ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcggatt
     6841 ttccgctcct gaaggcaagt tctcaacgaa aaagctggcg tgtttgatct tgccgatatc
     6901 atgataatag caagctactc gagttagtaa agaacgtcca ccgatttctg ctacagcatt
     6961 agcacttaga ttggctacca tcatgctatg gtgatacgta cctggtgctt cttccagcaa
     7021 ctgtttcaat aaaggatgat tgggattgct gagttcattt aatacgatca cactatcatc
     7081 attgaccatc aattcaatat aggggtgcag acctatcgtc agcaagaatg aaaacagtgc
     7141 acctgtgaat ccgcagacta gcgttaacca agtcgttgca tccccaaagc tcattccttg
     7201 gtagataatc aaaatgacat ccatcatcac aggaaaaata atgacccaca tagcagcgga
     7261 taacccttgt tctgagatcc gtttgcgtct gacgatcgtt gccatgagac cagaaaatac
     7321 ataggacatc aaaataactg tcagaaagtt cgtgccaatc gaatcataaa aaatgaaaag
     7381 tgcggatact gcttgaaaaa taccagccat gatccctgct cttcgattca agaaaaagct
     7441 taaaacaagt ggagcaaaag ccgcggggta aaacagcgga atataagcag cttgttctgt
     7501 ttggaagagt tggaaaaatt tcattagtac gacgcttact gacattgccg tcacgtagaa
     7561 aagtacgtat tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac
     7621 accgatttga agcaaaacag ctaaaaccat ggcaatgagt gggaaaatag aagtcgtttg
     7681 attcgtcaat cctagtaatt ccaatttttt catcgctgaa gcatcaattt gatttccttc
     7741 gcgaacaata atttctcctt ggaagatcat cactggttgt acagcttctc gtgcagattg
     7801 ttttaattct tctgtctttt tctcattcaa tacatcatta actacgatgc cttgatccac
     7861 taagtagcga atcatttgtt gctgctctgt tgtgacattc aaatattgaa tctgatcttc
     7921 tgcttcttgt ttaaaagctt ccaattcgct ttcacgaact tgtttactca tctgttcgtc
     7981 aatgagcttc aaactttcat ccctaactgt gtgaatttcc gtttcagtca tttcaaatat
     8041 cgtcgaataa aaattgttcg gcagtttttg gtaaaacgca acgttttctg cattcacgtt
     8101 ttcaaaattt ttcttcagtg tagcaatccg ctcttctgta gtaggtttcg ggatattttc
     8161 atcttccttc gccttatcta ccttgccatt ataagatttg ttgatggtat cattcgtctt
     8221 atcgatcaat tcgaataatt gcttgatccg attgttctgg tcatctgcca gatctttttg
     8281 ataagtatat tctggagtca ctgcttctgc tgccagtttt cttctttgtt cggtttcttc
     8341 cgtgttctca atcgttttat tcgcacggat actctcttct gctacttggc cttctctgaa
     8401 atcaatggcc ttttgtttga cactactaaa tgtaacgccg cagacaatga taaaaaacaa
     8461 gaacagaagg aaaggcaatg ctgcctttcc gagtttttct tgtagtttct gcagtgtttt
     8521 attcaacatg tgcttatcct cctaagcaaa gcttattctt gatgaagatc cgccttttca
     8581 taagctttga tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta
     8641 acaaaatcga tttttttgat ttgctttaat gttcgttctg catgaacaag accacttgtc
     8701 gtcccacgag gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa
     8761 ccgagacggg tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag
     8821 ataacgaatg catcatccaa tgtccgcccc cgcatgtaag ctaaaggtgc gatttcgatc
     8881 acgcctcgtt ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca
     8941 taaacaggcc gcagataagg atccactttt tctttcagat ccccgggtag aaagcctagg
     9001 ttttcccctg cctccacagc gggccgcgtc aaaatgattt tttgcacttc gccttttttt
     9061 agggcagcaa ttgctaatac tacggcaaga aacgttttcc ctgttccggc tggccctaca
     9121 ccaaatacga tgtcatgttt ggctaccgat tcaacgtatt tcttttgacc gctgtttttt
     9181 acccgtatag gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa
     9241 taatctaatg tacctttttg ccccattttc aatgccgtca caacatctgg cgtacttacg
     9301 tggattcctc gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc
     9361 tgggattgct caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt
     9421 gtttcctcga tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca
     9481 gcagctgtca atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt
     9541 tttttcgatt cattctttcc tattttacca tactcagaag aaactatata gcaaataaga
     9601 aaaggttgta aaagagtcgc ttaaccatac gtaccaagaa acgttaaaaa atagccgttt
     9661 ccattcgcta aaggtctcgt tttgataaaa agaagctgag aatgtaagtt atatgaaagt
     9721 ggagaacaaa atatttcgtt tcattcaaca agtattctct aggtattttt cctgtttcaa
     9781 aaaaagaaaa caaaatagtt aaaatattct tgcatttcaa aaaaaacagg tgtattatta
     9841 cgtcaacaaa taaacataca gcaaagcgtc gaggaaaaca gtaggctatt caacgattta
     9901 ttcccagtga gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc
     9961 gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac
    10021 tttatcaaaa agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt
    10081 ctagaatcta gagccctttt cccaagtgga aaagggcttt tttttatttt aaggtggtgg
    10141 ttttcatgga atctgatatt agtggcatta ctaattgctg caacgtttat atcgtcaatc
    10201 aaaatgataa ttattggagg aaaaatttat ggaagaaaca aaaacttatt catggaaaca
    10261 actattgatg gtcagttcac ttatcttcgg gatgttcttc gggtcaggta atctaatctt
    10321 tcctgtccac ttaggtcaaa tggctggcgc aaactgggtc agtgctggcg taggatttgc
    10381 tatctcaggt gctcttttcc cattactagc tatcttagct gtagtagtga caaaaagtga
    10441 tggtctgtat gacttagcta aacctgtcgg aaaatggtat gctgccttgt tcttaatcct
    10501 tgttcatttg acgatcggac catttttcgg tacaccacgg acagctgcta ctgcttttga
    10561 aatggctgct aaaccatttt tgcctgaaaa atataattca ttaggtatgt ttttattctc
    10621 tgctcttttc tttttacttg cttatttcct tactgtccat ccaacacggt tggtcaaata
    10681 cgtcggaaag tatctaaact ccttgtttct tgcattatta gcaatcattt ttgtcgtttc
    10741 tttattccat ccaatgggaa atctaaatca atctgctgca gatacttatc aaacaaacgc
    10801 tttaacaaat ggtgtcctag aaggttataa cacggttgat gcagtagctt tgcttgcctt
    10861 aagtgtcact tttgtccatg cagtccgtga ccttggtttc aaagataaaa aagtcacaga
    10921 attaacggca aaagctggta gcctgagcgt tgcgctagaa gtcatgatct attttggact
    10981 tgttcttctc ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg gcggaactgc
    11041 actttctcag atcactaatt tttatttagg taacttcggc gctagcttct taggagtgat
    11101 ggtcacatta ggtgtcttca ctaccgctat gggactcgtt gtttcttttg cgcaagattt
    11161 ccataaactg ttccctaaag tcagttatat gacatggctg cggttaacga ctttcgtctc
    11221 ttttgtcgtt gcaaatgctg gtttagacaa tatcatccaa tggtctttac cagtattgat
    11281 gcttttgtat ccattgtcgc ttgcgttgat cttgctttcc ttaacagcaa aatttttcca
    11341 aaagacacca tttgtctatc aagtaacgat gttgtttgct gctgttcctg ccgtattgga
    11401 tatgctggca aattcgcctg ctttagtgag tcaacaaaga gttgtagcaa gcatgctcga
    11461 attttatcat catcacgttc cttttgctgc attaggactt ggctggatgg tcccaacgtt
    11521 acttggttac gctggcagcc tactgtttta ttacgcctat cgcctatcag ggtataaaca
    11581 agaagcaaat gaactaccag aagaataacc aaatcaaaca caatttaaca taagaaatat
    11641 taaacaggaa aaaaccagct agatgaaaga atcctattct ttcgtctagc tattttttag
    11701 ttctcttttt tacgcgcaca aaaaaactgc ggacaatgat tgtccgcagt ttaaatgggc
    11761 tctataatat ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata
    11821 aacgcaaaca gacaccttga aatcctttag aataaagtcg acgaaaacca ttcaaaggag
    11881 tgtttcaaga tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat
    11941 cttatctttc atgaagatgc ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt
    12001 tttgatggca cattgaccta tcaaccagag gaatgcttcc attgtcacta tcaaaataag
    12061 caaacgatca ttaaatgggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac
    12121 tacaagacca ttctgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact
    12181 tttttagcag aagattcagt tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa
    12241 gccattcttg agctattaag cgaacctctt tctatgtctt tgatcgcacg tatgaagcac
    12301 atttctccga ccactgtcat tcgaatactt cgcagccttc ggcctaaaac cgtttctttg
    12361 aaccagccgt taccagaagt ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg
    12421 ggagccatga gctttgtcat gatggacggc aagacccatc aactgattga tattgtggaa
    12481 aatcggcaac taaatccttt acgcgactat ttcttgcgct accctagaaa agtccgtgaa
    12541 caagtacgtc tagtggtttc agatttctat acaccctatc gcacattggt gaaagagtgc
    12601 tttccgaatg cccgaatcgt cgccgatcgc tttcatatta gccaacacat cggtcgggcc
    12661 tttaccaacc accgaattca agtaatgaag acctttaaaa aaagggatcg tcggtcgaag
    12721 cacttaaaga aatactggag actacttcaa aagaatgcgt gggaattgaa aggtcagcat
    12781 cgctactggc gtccgtcatt tcgagatcat ctgacagaag cagagatagt cgatcggcta
    12841 ttatcttatg atgactcctt aaagcgaggc tacgaagtct accaggattt tctttccgct
    12901 atcagaagac aagatgttcc tgaattcgtc gcgctcctaa aagaagacta caaggagttg
    12961 ccagagcact accaaccagt gttcacgacc ttcaagaaat atcgaacaga aatcaaacga
    13021 gcgttgcgag ttccctattc caacggccct atagaatgtt tgaacaacca tatcaaagta
    13081 ctaaaacgaa ttgcctatgg ctttagaaac tttcaaaact accaagaacg aatcttttta
    13141 tatcgaggaa aatattttaa gaaaactaag aacactaccc aactgactaa agctagaaca
    13201 acaacacggc tagacaagat agctgtctag ccgtgtgagt atctcgatta ttctattgaa
    13261 tttcggctct atactttttg atgttggtga acttcaccaa catctttttt gtataaaaaa
    13321 atatagaaaa gacaccctga aatcctttag aattaatttg gctaccaatt cataggagga
    13381 ttcaagatgt ctcatactca gcttatcaaa gataccctaa atattctaga cctaaatatc
    13441 tatttcgagg aaaattgttt aactaaagaa aaatacaaag ggcaaatttg cttgatttac
    13501 aaaggaactc ttctgtacaa gccagaagag tgttcccatt gtctttgtgt cgtcccctct
    13561 aggatcattc gttgggggac gacaaccgtt cggttgttac tgaatgatgt gtctgagtat
    13621 cgcacttatc tggagctgaa gaaacaacga tttaaatgta agtcttgcca aagaacattc
    13681 gtagcggata cctctgttgc tgaaaaacac tgtttcatta gtcaaaaagt tcgttggtct
    13741 gtcattgctc gcttaaaaga gaatacttcc atgacggaaa tcgctcgtca gaaaaatatt
    13801 tccacttctt cggtctatcg tgtcatgaag cgattctacc gcccattgaa tcctttcaaa
    13861 cagactttgc caaaggtctt atgctttgat gaattcaaat cggttcgaag tgttgccagt
    13921 gcaatgagtt ttatcatgat ggacggacaa agccatgcat tattggatat tgtcgaaaat
    13981 cgacgcttgc cttatttaga acgctatttt tctcggtttt cattagcgac tcgtgaagcc
    14041 gttcaattga ttgttattga tatgtatgcg ccttatgttt cgttggtgaa aaagcttttc
    14101 ccgaatgctc agttgatcat cgatcgtttc cacatcgtcc aacatattgg cagaactttc
    14161 ttgaatcatc gagtgaaaga aaccacggta cgacttaaag gaaattccca ctctcaacga
    14221 gggttaggga aaaaattgaa gcgctattgg aaactcctac aaaaagaaga agcaaagtta
    14281 aactatgaaa aacgggtttg gcgtgccagt tttaaagatt atttgactga aagtgagatt
    14341 gccgatcgac tgttagctgc ttgtcccaac ttacgccaag gctatcagtt ataccaagac
    14401 attctttatg cggtaaagaa aagagatcag tcactatttg aggattgttt aactagagac
    14461 attagcggtc tgccagaaac ctatacaacc actttacgaa cgtttaaaaa gtttctgcca
    14521 cagatcca
//
LOCUS       DASJTM010000070        14354 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJTM010000070 DASJTM010000000
VERSION     DASJTM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.070"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..700)
                     /locus_tag="U4B29_002048"
     CDS             complement(134..700)
                     /locus_tag="U4B29_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2106989.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(700..1656)
                     /locus_tag="U4B29_002049"
     CDS             complement(700..1656)
                     /locus_tag="U4B29_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN2106990.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1656..2312)
                     /locus_tag="U4B29_002050"
     CDS             complement(1656..2312)
                     /locus_tag="U4B29_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2106991.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2682..4700
                     /locus_tag="U4B29_002051"
     CDS             2682..4700
                     /locus_tag="U4B29_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2106992.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4770..4976
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5127..7541
                     /locus_tag="U4B29_002052"
     CDS             5127..7541
                     /locus_tag="U4B29_002052"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN2106993.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7889..9442
                     /locus_tag="U4B29_002053"
     CDS             7889..9442
                     /locus_tag="U4B29_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2106994.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9823..11607)
                     /locus_tag="U4B29_002054"
     CDS             complement(9823..11607)
                     /locus_tag="U4B29_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106995.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11622..13343)
                     /locus_tag="U4B29_002055"
     CDS             complement(11622..13343)
                     /locus_tag="U4B29_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2106996.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13443..14204
                     /locus_tag="U4B29_002056"
     CDS             13443..14204
                     /locus_tag="U4B29_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2106997.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4645 a         2673 c         2865 g         4171 t
ORIGIN      
        1 gatttggttg gacttattac cgcagatata agcttttgag caccgtgaat aaagaggtta
       61 tgaaatcaac gctctaacct gagtattaga gaaaaaaacg caaaacatat catttatgtt
      121 ttgcgttttt tatttatctt tttttctcaa tacaatataa aataggcggc tggttctttt
      181 ggttgataaa ccgataattc agcacattat atgtttcctg tggcagtttg gaacaaaaag
      241 aatcaaccat atccagttct ttttctccac cttcatgacc atagtagacg acaaggatca
      301 atcgtccgcg aggcacaagt cgtttcaata tttcttccat agctgtgcgt gtagtctctg
      361 gcatagtgat gattgcttta tcactttttg ggagataccc tagattgaaa atccctgctt
      421 tgagattttg ttgttcagct atcacttctc ctaacgtttc gtggccttgt agaaacaatg
      481 agacacgttc ttctaactga cgctgttcta gcctttcacg agtagcatcg attgcttgtt
      541 gctggatatc aaaactatag acatgaccag tttttccgat gtgttctgct agaaaaaccg
      601 tatcgtggcc attgcccatc gttgcatcta cgacgtgatc ccctggttgc aagatttctt
      661 gcagtaatgt atggctgaaa tgcaaagcag tttgcaacat cagacgctca cctttttccg
      721 tacatcatat tttccttgga agctatctct gcgaagaagt tcttcatcaa ttgcattcaa
      781 gacttcccat tttttcaagc tccacattgg tccaatcaag gaatcccacg gcgcatcccc
      841 tgtcaaacga tggatgatga tctcttttgg tatcatttcc agctggtcgc aaatgacatt
      901 gacatagtca ggacggctca ttaattgaag gcgaccttca tgatagtccc gcaacattct
      961 cgttttgcgc atcagatgca tcaaatggag cttgattcct tggatatctg aatctaaaat
     1021 ggtgcgtctg acattttctt tcatcatttc taaactttcg cctggtaatc cgttgatcaa
     1081 atgcgtacaa acacggatat tatgtttgcg taatttagcc acgccatcta aataagtttg
     1141 ataatcatgt gctcggttga tcagttttga agtttcttca aaagttgttt gcaatccaag
     1201 ttctacccat aaatacatcc gctcgttcag ttcagcaagg taatcaacta cttcatctgg
     1261 cagacaatcc ggtcgtgtac cgactgataa tcctacgaca cctggtaaat tgactacttg
     1321 ctcaaatcgt tctttgatta tttcaactgg cgcatgggtg tttgtaaaat tttggaaata
     1381 aacgatgtat tgttggacat gaggccattt ttgatgcatc atgtcgattt ctttctggaa
     1441 ttggattgga agagggtctt caggtgcaac gatcatgtct cccgaaccag atacactgca
     1501 aaaagtacaa ccgccatggg caacagtccc atctcgattc ggacagtcaa atcctccatc
     1561 gatcggcacc ttgaaaattt tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg
     1621 atatctttta tttccttctg aataaggaaa agtcattaaa agtccttctt tctgtaagag
     1681 ttatctattt gttttctttt ttctctatac actggataag tcaggcacag ccaagaaaga
     1741 ctgagtaagt aaccgccgat gatatcagta ggaaaatgaa cgcccagata aatgcggctt
     1801 atcccgacac ttactataat aaacatcatc agcaattgaa gcaatgtgcg taaggttctt
     1861 ttttgaacaa aagctggaag cagaaatagt aaagaaccgt aaagaatcat actgcctgct
     1921 gcatgtccac tagggaaact gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc
     1981 tctcgcatga aaaatagttt gatcaccggg ttgatgattc cagcaattac aataaatcca
     2041 gaaacgaacc aaaatgtttc tgcccttttt ttcttatacc acatccataa agccgcacaa
     2101 gcaaataaaa tgatgacggt aagaggatta ccgaatttag tcataaagag ttggtagctg
     2161 ttcagatcag gataagaagc tctgacaatg cctgtaaacc actgatcgaa accagaaagc
     2221 cacgacagat ggaactttac gatccagccc aaaatgaaaa acaggacaag aaagaagcta
     2281 ccagccacct gaaatgatgt tttatatttc atacaattcc tcctttgaag caatgactta
     2341 ttatagcatt tttggtccag cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc
     2401 tctttgaata accaatattc ttataaaaaa aataactgta atcggaaaga cattttttgg
     2461 taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag
     2521 aacgtagacg taggaagaac ttgaaaatga acagaagttg ttcattacgg taaattacaa
     2581 tatgattaca aaaaacaaca aagctcttaa ataactttta ataaatatta agagaagtta
     2641 taattattaa taatagataa attgaagttg gaggatagaa gatggaaaac attgcaagaa
     2701 aagaacggag acgtttagaa cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa
     2761 tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc attagtacag ccggttcctg
     2821 ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat caatacagcg gcatttatca
     2881 cagaaattgc tacttatgct caaccaatcg ctcaagccaa cgatctatat gcatcagtga
     2941 tgatcgcaca agcagttgta gaaagcggtt ggggcagtag tgcgttatct caagcacctt
     3001 attataatct ttttgggata aaaggcagct atcaaggaca aaccgtctac atggatacgc
     3061 ttgaattttt aaataatcaa tgggtaaata aaaaggagcc tttccgacaa tatccttctt
     3121 ttgctgaatc tttcaatgac aatgcctatg tacttagaaa tacttcattt ggcaacggtt
     3181 attactatgc cggtacttgg aagagcaaca cgaaatccta tacagatgcg acagcttgtt
     3241 tgacagggcg ttatgcgact gatcctggtt acgcaggcaa attaaataat atcatcgcaa
     3301 cttacggatt gacccaatat gatacaccag ctagcggaaa cgcaggcggt ggagcgacta
     3361 caggtggcaa taatactgga agtgcaggta gtactggcgg ttcaagcaat tctggtaata
     3421 caaactcagg ttcagcaact tcgaccactt atactgtcca atctggtgat tctgtttggg
     3481 gaatctcaaa taaattcggt atcacaatgg atcaattgat ccaatggaac aacatccaaa
     3541 acaatttcat ttatccagga caaaaactga caatcaaagg tagccaggaa aatggctctt
     3601 cgacgaataa ttccggtaac aataccaatt cttcaggaaa tgctggtaca tcaaacggag
     3661 gacaaacaac tggtgcgaaa tacactgttc aatctggtga ttctgtttgg aagatctcaa
     3721 atgatcacgg aattacgatg gctcaattga ttgaatggaa caatatcaag aacaatttcg
     3781 tttatccagg acaacagcta atcgtatcaa atggcggttc ttctaatgct ggagcaaata
     3841 ctggctcaaa tactgggaat acaagtggct ctagttcgtc cagctcaagt tcttctaata
     3901 cagcttcagg aacgaaatat acagttaaag ccggagactc tgtttggagc gtctcaaata
     3961 aatacggaat tacgatggct cagctaatcc aatggaacaa tatccaaaat aactttatct
     4021 acccaggaca acaactgact gtttcaaaca gtggatcaac aaacagcacg tcaggtaata
     4081 caggttcgtc aaccaataca aataacagca gttcagttaa tactggcagc agttcgtctc
     4141 aaggcactta taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca
     4201 tcacgatgga tcagttaatc gaatggaata atatcaaaaa caacttcatt tatccaggac
     4261 agcaattgat cgttaaaggt ggaacttcgg caaatacaaa tacaggttca actacttctg
     4321 cagcgaaacc aaatacaccg aatacgtctg ctacgacaag tacatcatcc actggtaata
     4381 cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa catcatatca
     4441 cgatggatca gttaatcgaa tggaataata tcaaaaataa tttcatctat ccaggacagc
     4501 aagtgattgt gaagaaaggt tcatctcaat caacaaacca acaatcagtt actggttcga
     4561 aaacctatac tgtgaaatct ggagaatctg tttggagtgt tgctgacagt catggaatta
     4621 caatggctca actaatcgaa tggaacaata ttaagaacaa ctttatttat ccaggtcaaa
     4681 cgttgattgt agctaaataa aatagctgtt cgaagaaata acgattgaat tccacaaaaa
     4741 tatttgttac tataaggcta cttaaatatg acaacacttg aaaaggaagt agtaaaaaga
     4801 ctgaagtttc agagagcgca tggctggtga gaatgtgcac ggatgctttt gaactcgcct
     4861 ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt
     4921 cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt agattacgtc ctataaaaag
     4981 agcaatcttt ttataggatt tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa
     5041 ggaaataaaa ataaacagat tctgccggtt ttttcgttat atttataata gaaacaagtg
     5101 ttttcaatag aacaggagga cagcaaatga gttacaatca taaagaaatt gagaaaaaat
     5161 ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca tgatgatcct gaaaagccaa
     5221 aattttacgc actggatatg tttccctatc catccggtca agggctacat gtagggcatc
     5281 ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg
     5341 tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc tgaacaatat gcgctagata
     5401 caggaaacga tccagctgag tttacccaaa aaaatatcga aacattccga cgccaaatca
     5461 attctttagg atttagttat gactggaatc gcgaagtgaa tacaactgat ccggagtatt
     5521 ataaatggac acaatggatc tttacgaagc tttatgaaaa aggtctagct tatgaagcag
     5581 aagtagcggt aaactgggta cctgaattag gaacagttat ttcaaatgaa gaagtgatcg
     5641 atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc caatggatgc
     5701 tgaaaatcac tgcttatgct gatcgcttat tggatgatct agaattagtt gattggccag
     5761 aaagcattaa agaaatgcaa agaaattgga tcggtcgttc agtaggagca aatattgaat
     5821 ttaaagtggc tggtacggat aaatcctata ccgtatttac gacacgacca gatactctat
     5881 tcggtgcaac ttactcggtt cttgcacctg aactagatct tgttagagaa attacgaccc
     5941 ctgaacaaaa agctgctgta gaagcttata tcgaagagac agctaaaaaa tcagatctaa
     6001 agagaacgga tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat gcaatcaatc
     6061 cagttaatgg caaggaaatc ccaatctgga tcgccgatta tgtattatct tcatacggca
     6121 caggagccat catggctgtt ccagcacatg atgaacgtga ctttgaattc gctcaaactt
     6181 tcggactaga aattcttcca gttatcgaag gtggagatgt tgaaaaagct gcttacacag
     6241 aagatggtac gcacatcaac tctgaatttc tagacggcat gaacaaacaa gaagcaatcg
     6301 ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct
     6361 tacgtgactg gctattctcc cgtcaacgtt actggggtga accaatccca atcatccatt
     6421 gggaggacgg aacagtcaca gctgtcccag aagaagagct accattacgg ttgcctaaga
     6481 caaaaaacat caagccaagc ggtactggag aatctccatt agcaaatatc gaggagtggg
     6541 tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac
     6601 aatgggcagg aagctcatgg tattacctgc gttatatcga ccctcataac aaaaaagaac
     6661 tagcagatta tgaaaagttg aagcgttggc taccagttga catctacatt ggtggagcag
     6721 aacatgcggt acttcattta ctatatgcac gattctggca taaattctta tacgatatcg
     6781 gtgttgttcc tacaaaagaa ccattccaaa aactatacaa tcaaggaatg atcttaggcg
     6841 aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg
     6901 aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt catggggcca ttagatgctt
     6961 cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa attcttggat cgtgtatggc
     7021 gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat cactacattg aatgatggca
     7081 agcttgataa agtgtatcat caaacagtga aaaaagtgac agaagactat gaaaatcttc
     7141 atttcaatac agcaatctcc caattgatgg tctttatcaa tgaagcctat aaagtggacg
     7201 ctctgcctta tgaatatatc gaaggatttg ttcaactact cgcaccgatt gctcctcaca
     7261 tgggtgaaga actatgggca atccttggaa atgatggtgg tatttcttac gcaccatggc
     7321 caacatacga tgaagcagca cttgttgaag acgaagtaga agttgttttc caagtaaacg
     7381 gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg aaaagatgag ctagaaaaag
     7441 cagcattagc caatgaaata gtgcaagaat atattgaagg caaaacagtc agaaaagtta
     7501 ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta attggctctt tgtcaataag
     7561 gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga acttcattct gtctcagatt
     7621 ttttgttatt tcaaattgat taattgattg atcaaaaagg ttttgatttt catgttctca
     7681 aacacgttca atgtcttttt cttctgaatg gtcttgtaag agatctctgt aatcatcaaa
     7741 tttttgtcta ttaatcttag tattttttga tgtataaact acctgaaatt gagagtacat
     7801 accaaattca taaatttttt attagtggga ctagtgattg tatggatttt gacgcataat
     7861 gaatgaagta tgagaaaagg agagacaaat ggatcaagaa ctatttatta tcgaagtatt
     7921 atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa
     7981 gcttttaagc ttagtatttt tctctggttt gctttttttc ggagctgtat cgttcctata
     8041 tcgccatatg agtgcagtag tccgttcagc tgaagggatg caaaaattaa ataatttgta
     8101 tcacttttcg tggggagttg gcggaataac aattgttgtg cttattgttt ttcttctact
     8161 tatttggctc cgttaccatc aattgtcgcg tcatttcgat caatggctta gtcaaagagc
     8221 acaacagttt ttacataata aaccaacttc tcaagatgat gactatcttt attttgagaa
     8281 gacttcgacg agtcaagctt ttcggataca aaaaagtgat tgccagttat tgctgagatc
     8341 acttgatgat ttacctatct acttaggcga aagcaaatca aaattcggaa tgaacacatt
     8401 ccatattttt ttactagaaa acgaacccag atgggaaaaa agccagtctt cgcctggtgg
     8461 atacctgaca actaagatga gtacattagt cactggtttt ttcctcattc ttttagctgg
     8521 aacaatctat tatgggagat acactcagcc aaaaatgtct tctcatataa atgctgcttc
     8581 tcacatgaca atgaactcaa ctaacgatac gcatatacaa aagacaaatg aagggttgtt
     8641 agctgatcct aagcaaaagg taaatttgac aacacaaaag ataaatgatc tcaaacttga
     8701 tacagagacg cactctctct atatgacaac agatagtggt gctaattggg tatttgtgcc
     8761 gattgattcg caatggttgc gttttggcga atacaccctg acaacggggc tgattcctga
     8821 aggttattgg atggacaaca catatgatat ttccgccgat ttctcatggt ttatctattc
     8881 ttctggggat gatgtacgtc tgctgacttc aagagataat ggtaaaacat ggcaaacgtc
     8941 tattatcacc caatcagttc agccgatacg ttatcgaaaa gcggtatttt atggcaacga
     9001 tggtctattt atctgtacat cagccagtgg gatgtctgca gaaactttgt acatttacac
     9061 tactatggat ggcggaatca cttggataaa aagtggaagt acaatgatca atcagccagt
     9121 acaaaatgtc agcttcgtta caacttcttt aggttttgtt tcgacacgtg aaagtttgtt
     9181 ttatacaaac aacggaggac gctcattcaa agaatcagtt ctgtcgattc cggaaaacta
     9241 tcagacaggg gggatagatc tttttcaatc accaaatgaa gttactcaaa taagcgcgaa
     9301 tacgttagag acaaaatttt atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt
     9361 tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg caattcgttc agcaactatc
     9421 gcaagtagaa caaggagatt aaagaagaga gattgtgagc ctgacgttca gcaccgagtc
     9481 acaagaaaca aattcaaaaa acagcttctc atatttgttg aatagtgtaa ctcgtgattc
     9541 ggagttgaca agagcaacaa gaagcgattt tgaagtatag cttctcgtat tttacaagat
     9601 cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa agggattgtg
     9661 acaagtcttt tgtcactact taccgaacga aactgaagga gctatccaaa aagtatcatt
     9721 agtattattc tttgtgtttc tagtggcacc actagtacat ttaaaaaagc tatcgatcat
     9781 ggactaatca tacatgatcg atagcttttt agataatgtt tgttacgcta tctgaaattg
     9841 actattataa agactggcat aaagcgttgg tgattggagt agttcagaat gtgttccttt
     9901 ttcgacgata tcgccattct tcattacaag gatcatgtct gcattttcaa tcgttgacaa
     9961 acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca gcttccattg cttgctgaat
    10021 caatgcttct gttcgtgtat ctacgctaga agttgcttca tctaaaataa cgacaggagg
    10081 attcgctaga atgactcgag caattgtcaa taattgttgc tgtccttgag ataacgtgcc
    10141 gttttcgcta gaaatgatcg tatcatatcc gtctggcagt gttcgaataa agtgatcaca
    10201 ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg gcatccattt tcccgtaggc
    10261 gatattatca gctaccgttc cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga
    10321 acgacgtagt tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc
    10381 tttattcagt tcataaaaac gcatcagcag attgactaaa gtcgtttttc ctgcaccagt
    10441 tggaccaacg atcgctaccg tttgttttgg ctttactgaa aaatcgactt gattcatcaa
    10501 tattttatct gatgtgtaac caaactgtac atttttgaaa gctatttttc ctttcggttg
    10561 ttctaaaagt cttgcatctg gaaggtctgg aatttcatct gtttcatcta atatctcaaa
    10621 tatcctatcg attgctgcca ttgcggattg gatcgagttg atcacataag aagcggtaga
    10681 aattggttca gaaatctgat tgatgtactg aagataagct tgtaagaagc cgatcgtcac
    10741 accacctgat agaacaagca tagcaccgaa taccgcactt atgatgaagg cgagttgatt
    10801 aatgaaacga atggccggat agatagcaca gttcataaat tgagcacgtt taaaagcgtt
    10861 gtattgcttt ttatttactt cgtgtatagc agcttctgca tgagcttgct gattgaatgt
    10921 tttgaccact agattaccag ctaagatttc ttccattcga ttatttaaaa cacccagttc
    10981 atactgattc tgttcagaaa tctgtttgtt tttgttcgca atcttagttg taacaattga
    11041 actgccaacg atcagcaata agacgagcaa agtaagcttg ctttcgatat aaaacagcat
    11101 cagaccagca gcagctattg ttacgacaga agtaaaaaat tgattgatac ctgtcaataa
    11161 cacttgagaa agttgattga gaccggtagt tgtccgacta atgatgtcac ctacttgatg
    11221 attgtcaaaa aacgacatcg gtaatgtttt gaattttttt gtgatttctt ttcgcaccct
    11281 caatgtgatc ttttcgctta acgctgccat tgtccgttcc tgaataaatg aagtgatgct
    11341 gcttaagact gaacagccga tcagcaataa gacagggaac aacaatgctt gtttgatatc
    11401 actgaaagaa agctgtccaa tacctgtttt tcttatttct gataagagat catcgatccc
    11461 gattcccatg atcaagggca tggctacgac taataggttc ccgaataaac tgcatagaaa
    11521 caatccgtaa aaatatttat gttcaggttt gatcattttc caaaaacgta tcaaagcacg
    11581 cgtgttcaac gtttttttct tttccatcgt atcccttctt tctatattcc ttgtgatttt
    11641 gcaaactcct gataagtttg gcaattagac agtagttctt catgagtacc ttgtccaacg
    11701 atttttcctt catctaaaac aatgatgtta tctgcttgtt ggattgtact gaggcgttga
    11761 gcaacaatca cgagagtttt ctccgacatg tgtttatgaa gggctgctct taatgctgca
    11821 tctgttttat agtctaaagc ggagaaacta tcatcgaata catagatctc tgctggtttg
    11881 atcaacgctc gagcaataca tatccgctgt ttctggcccc ctgaataatt agaaccgcct
    11941 tgagcaacaa agctgtccaa tccgtcagga agggtgtcca ctagttcttc taattgagcg
    12001 atcttgatcg ctttagacaa ctcttctgtt gaggcatatt catttcccat ctgcaagttg
    12061 cttaaaattg tcccgctgaa taagaaagcc ttctgaggga cataactgat gtgggagcgg
    12121 atctgttctt gagtcatttg agtaagagga ataccaccaa gggttacttt tcctatcgta
    12181 gggtcactaa gtctcaagag aagtttcgcc aaactacttt ttccagatcc ggtcccgcca
    12241 acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag aactggatca
    12301 gagatttcag tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag
    12361 gttggttcag ctactgtttt gatcgtctct tcctcaattt cgatctctgc ttgcagtatt
    12421 tcttctatcc gtcgtaatga ggcaaacgat ttagggataa tgaccaatac catagctgcg
    12481 atcatcaagt aagcaagtgt cagcatagag tattcgacta cagcagtcac gacaccgatt
    12541 tgtaatgctc cttctgctac aagatttccg ccaaaccaca aaatagaagc ataaacgatt
    12601 cccatgagca aaaaagcaat tggagtgacc caagcaaata ttcggttcac tttgatcatt
    12661 tgattagcat agtcagcaaa gctcttattc gttcgctttt cttcatatga ttgattatca
    12721 aaggcacgga tcatattaat cccagtaaag aattctctca atgtgatggt gatccgatcc
    12781 atttttggct ggatactcaa agataaaggt gcccctcgtt tcatcagtcg ataaacaatc
    12841 catacataaa aaacaacaga gatcaatgta acccatgtta gtaatagaga ataacgaaac
    12901 atcatgagaa tggtgaaaac gcaaataacc ggtgctggta agatcatttg gcaaaataaa
    12961 acaatcattt gctggacatt atcgacatca ttcgtcattc ttgtcaaaag agatccagaa
    13021 ctaaaacgac tgatatcctt cattgacagc tgttggaatt tacggaaata tttctcccgc
    13081 gtttggaatc caaacctagc ggaagccaat gctgaaaagt aactagaggc aactgcagcg
    13141 attgttccta gaaacgctac aagcagcatt tcaactccaa ttttttttat aactgaggct
    13201 tcgccacttg ctatacctgt gtcgatcagc tttgctacta attttggtac acctaatgta
    13261 ccggcgattt ctaaacagat catcacaaag gtaaggagag cgagtccttt gtttttctgt
    13321 aagaatgttt ttaagataga catattattc ctcctttttt cctgatactt ttcgattcta
    13381 taccttcaag taacttgaag gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa
    13441 aaatgaaaaa aatgtatacg attggccaag tatcggaact atttgatttg ccaaaatcaa
    13501 cgatccgcta ttgggatgaa caaggattaa tccgttcaag tagacaagaa gagaatgatt
    13561 atcgattgtt tgatatcgat gatattttta tgctttatga tatcgttttt tatagaaaat
    13621 tagatatacc gatcaaacag atgaagaatt tatatggaaa aacattgacg gagttatatg
    13681 agacacttga tgaaacagaa agaagaatcc ataaagaact ggtggtgatg aaacaaaaac
    13741 aaaaagagat aagggagcaa aaaaaacagt tgaaattgat gatcgataca aacgaggaag
    13801 aatttcctgt agaagagatc ccttttgact gtatgatctc aacggagttt gaagatattg
    13861 tggaaatcaa aaaatttcta cctaactatt caagctttgg gatgatgagt atgccagctt
    13921 ccagttcaca gacgatgtat ggatttttta tcgaccctag tgaagtacat ttatttactc
    13981 aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg tcgattctta ttgaaatccg
    14041 agatgaacca tgcggaacgt aataatattt tagaaatcag agaaagaatg tatgaaaaag
    14101 gatggaaaac aggagaagtg atcggccagt atctattgac gaatacggat gaaaacaata
    14161 tcagaactga atattatcat gcatggatag aaatgaaaaa ataggaaata tgtgtttaag
    14221 ataactcgaa aatgatggtt gttttagtta taaagaaaaa ttttctggtt cgattacggg
    14281 tttaatgtgg gggattatta gtcgtggata gatagaattt tagtttttta gaggaaatat
    14341 gtctttaaaa atgg
//
LOCUS       DASJTM010000071        13996 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJTM010000071 DASJTM010000000
VERSION     DASJTM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.071"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            188..526
                     /locus_tag="U4B29_002057"
     CDS             188..526
                     /locus_tag="U4B29_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2106998.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(731..970)
                     /locus_tag="U4B29_002058"
     CDS             complement(731..970)
                     /locus_tag="U4B29_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2106999.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(946..1293)
                     /locus_tag="U4B29_002059"
     CDS             complement(946..1293)
                     /locus_tag="U4B29_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2107000.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2025..2606)
                     /locus_tag="U4B29_002060"
     CDS             complement(2025..2606)
                     /locus_tag="U4B29_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2107001.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2789..3415)
                     /locus_tag="U4B29_002061"
     CDS             complement(2789..3415)
                     /locus_tag="U4B29_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2107002.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3437..4768)
                     /locus_tag="U4B29_002062"
     CDS             complement(3437..4768)
                     /locus_tag="U4B29_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN2107003.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4923..5324)
                     /locus_tag="U4B29_002063"
     CDS             complement(4923..5324)
                     /locus_tag="U4B29_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2107004.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5410..5616)
                     /locus_tag="U4B29_002064"
     CDS             complement(5410..5616)
                     /locus_tag="U4B29_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107005.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5711..7213)
                     /locus_tag="U4B29_002065"
     CDS             complement(5711..7213)
                     /locus_tag="U4B29_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN2107006.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7231..7686)
                     /gene="argR"
                     /locus_tag="U4B29_002066"
     CDS             complement(7231..7686)
                     /gene="argR"
                     /locus_tag="U4B29_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2107007.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7784..8401)
                     /locus_tag="U4B29_002067"
     CDS             complement(7784..8401)
                     /locus_tag="U4B29_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN2107008.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8622..9995
                     /locus_tag="U4B29_002068"
     CDS             8622..9995
                     /locus_tag="U4B29_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2107009.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10070..11182
                     /locus_tag="U4B29_002069"
     CDS             10070..11182
                     /locus_tag="U4B29_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN2107010.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11254..12891)
                     /locus_tag="U4B29_002070"
     CDS             complement(11254..12891)
                     /locus_tag="U4B29_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN2107011.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13059..13667)
                     /locus_tag="U4B29_002071"
     CDS             complement(13059..13667)
                     /locus_tag="U4B29_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2107012.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3824 a         2918 c         2479 g         4775 t
ORIGIN      
        1 ttattggata taaatagaaa taaattgtag ccagttcatt ttttctttaa ctaaaaaaat
       61 aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata gctaaccccc
      121 cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga caacaggcca
      181 tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg acaaatttca
      241 attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga tcaaagaatg
      301 cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact ttgctattgt
      361 agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc caactcatat
      421 caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa ctgccaacca
      481 gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat aaacatcgtt
      541 aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct atacattaga
      601 aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt cttctgttct
      661 ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa
      721 atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta atgatttttc
      781 gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag acaagcggca
      841 ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg attgattctg
      901 ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt ttttccttat
      961 ttgaaaacat aaatttgtct ttttcttcat tccactcatt gggaaaacac aacgattttg
     1021 caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg cattaatcat
     1081 caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag tgaggatgtt
     1141 ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc cagctgctaa
     1201 ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga taataattct gaaggagctc
     1261 tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaatc tttttttcta
     1321 tcatacactg caacaagaag ttcgcaccaa caaaacgctt tcaatttttg tagaactaaa
     1381 aaagttgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa ctcctcggtc
     1441 acaagtcaca atattcaaca aacatgagaa gctgcttttt gaaattgttc cttgtgactc
     1501 ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc agtttctcgg
     1561 tattaagcca aatttatgca aaaatgtaga aagctatttt cacaaaattt ttcttaatac
     1621 tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
     1681 gcggtaataa gcccaaccaa acgaaaaata tgagcagcta tttttatttg gctgttctta
     1741 ttacttgatg cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctaa
     1801 ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc
     1861 taatactcag gtcaaagcgc tgatttcaca acctctaata cacggtgttc cagaagtagc
     1921 ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct
     1981 tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag aaccttttgc
     2041 gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga tagatcctgt
     2101 gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt ggcttgacgg
     2161 tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg tttcgtatac
     2221 ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc cactgtactc
     2281 aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt ctcatgatca
     2341 gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc agcatttaag
     2401 atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac cgctagacaa
     2461 acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc agaagctgct
     2521 agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc tttggtcgct
     2581 tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc atagtcctat
     2641 attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt
     2701 atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca ttgattcaag
     2761 aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact tcctctgttg
     2821 tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc
     2881 caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct accaattcac
     2941 gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact gtgtcgattc
     3001 cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag
     3061 agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa tcggctcctg
     3121 aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc tcaagcatca
     3181 tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa taatgaggag
     3241 aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc atattttcga
     3301 tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca
     3361 agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg aacatggtgg
     3421 attctccatt cgttatttat tttaatacat catttagagc ttcactcatt gtcggatgag
     3481 aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca
     3541 agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt
     3601 cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt gctttaggga
     3661 caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt
     3721 ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc acagctttcc
     3781 ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt cgataatcat
     3841 ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt
     3901 ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat
     3961 ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt
     4021 ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt
     4081 cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca
     4141 tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat tcagaatggg
     4201 catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa
     4261 tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg
     4321 tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag
     4381 aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca
     4441 cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct
     4501 catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt tctttccgag
     4561 caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa
     4621 tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa
     4681 ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt ccaaaaccga
     4741 tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg
     4801 gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa
     4861 aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc
     4921 ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag
     4981 aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc
     5041 acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt
     5101 gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca
     5161 ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg
     5221 attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg
     5281 cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg
     5341 attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa
     5401 aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa
     5461 aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac
     5521 ataatctttt atatgattga ttttaagata catatatacc agaaagttga ccccataaac
     5581 caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc
     5641 ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa
     5701 aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg
     5761 ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta
     5821 gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta
     5881 gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca
     5941 acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca
     6001 attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta
     6061 atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg
     6121 caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta
     6181 gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc
     6241 cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag
     6301 aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta
     6361 atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac
     6421 gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct
     6481 tgatactttc catcgcttct ttattatcag gatttgggat atgattagga aatcggccat
     6541 ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag
     6601 caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt
     6661 tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg
     6721 tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg
     6781 ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc
     6841 cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact
     6901 gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat
     6961 gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc
     7021 tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt
     7081 tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta
     7141 cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt
     7201 tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc
     7261 aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat
     7321 cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc
     7381 atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg
     7441 tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt
     7501 atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg
     7561 agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc
     7621 ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt
     7681 tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata
     7741 ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt
     7801 taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa
     7861 tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt
     7921 tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac
     7981 cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac
     8041 gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt
     8101 gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc
     8161 attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc caattttttt
     8221 taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt
     8281 cttcctgatt cgggtcgccg atccctctga tgctaaagaa tgatttttta ggaacctcta
     8341 gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca attttttcca
     8401 tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac
     8461 ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat
     8521 cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa
     8581 atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc
     8641 tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg
     8701 agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac
     8761 aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta
     8821 cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct
     8881 tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat
     8941 tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat
     9001 ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg
     9061 ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca tggaatcata
     9121 tcttcctgtt tcttgtgccc gttttgtttc tttctttgat tatcggctgg tttgctattc
     9181 ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc
     9241 ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg
     9301 ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc
     9361 atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct
     9421 tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga
     9481 tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg
     9541 gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa
     9601 tttttgctgg gctgattctt tctttgattg gttggctagc attattccta ctcataggac
     9661 acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat
     9721 cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg
     9781 ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag
     9841 cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat
     9901 taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat
     9961 tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat
    10021 ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat
    10081 tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa
    10141 attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat
    10201 tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg
    10261 cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca
    10321 atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga
    10381 gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac
    10441 atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg
    10501 tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga
    10561 gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc
    10621 gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt
    10681 attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt
    10741 tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga
    10801 tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg
    10861 ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt
    10921 cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc
    10981 ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc
    11041 tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc
    11101 caacttctta aatactggtc attggcctac ggaagattat caaaaggaca tctccaccta
    11161 tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt
    11221 gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc
    11281 caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg
    11341 taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac
    11401 aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc
    11461 tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt
    11521 atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc
    11581 tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc
    11641 aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt
    11701 tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg
    11761 aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac
    11821 tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc
    11881 tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag
    11941 gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg
    12001 ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat
    12061 cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga
    12121 caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc
    12181 gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt
    12241 ttttaattct ccaaagtaac gtaatcctag cacagccgct gtattatgga gagcatcaaa
    12301 caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag
    12361 atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg
    12421 ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg
    12481 actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg
    12541 cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc
    12601 taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa
    12661 tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc
    12721 tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc tatcaaaaac
    12781 ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag
    12841 tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc
    12901 tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac
    12961 tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata
    13021 aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac
    13081 cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca
    13141 cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg
    13201 ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat
    13261 tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca
    13321 actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct
    13381 gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt
    13441 aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca
    13501 tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat
    13561 tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca
    13621 ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt
    13681 ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag
    13741 gtaaaagaaa catgacattt aatgacaaaa taacgaatta tttaatattt gtaacattta
    13801 aacaaatatt tttttgatac aaaaccataa aagccttgtt gtataagcaa agtaatcgtt
    13861 tttctatgtt tttttatttt tgaatgaaaa acgcagattc tgtaacattc actgcaataa
    13921 tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa
    13981 aaatacaaaa aaacca
//
LOCUS       DASJTM010000072        13839 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJTM010000072 DASJTM010000000
VERSION     DASJTM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.072"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U4B29_002072"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U4B29_002072"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN2107013.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="U4B29_002073"
     CDS             complement(2261..2512)
                     /locus_tag="U4B29_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN2107014.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="U4B29_002074"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="U4B29_002074"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN2107015.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="U4B29_002075"
     CDS             3462..3680
                     /locus_tag="U4B29_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107016.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="U4B29_002076"
     CDS             complement(3969..4481)
                     /locus_tag="U4B29_002076"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN2107017.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="U4B29_002077"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="U4B29_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN2107018.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="U4B29_002078"
     CDS             complement(6934..7377)
                     /locus_tag="U4B29_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN2107019.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="U4B29_002079"
     CDS             complement(7378..8166)
                     /locus_tag="U4B29_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2107020.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="U4B29_002080"
     CDS             complement(8163..9104)
                     /locus_tag="U4B29_002080"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN2107021.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="U4B29_002081"
     CDS             complement(9281..9868)
                     /locus_tag="U4B29_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN2107022.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="U4B29_002082"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="U4B29_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN2107023.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="U4B29_002083"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="U4B29_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2107024.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="U4B29_002084"
     CDS             complement(12785..13720)
                     /locus_tag="U4B29_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2107025.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3823 a         3099 c         2440 g         4477 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataaccc gcgaattgta aaattaagtt agaaaaata
//
LOCUS       DASJTM010000073        13404 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJTM010000073 DASJTM010000000
VERSION     DASJTM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.073"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..606)
                     /locus_tag="U4B29_002085"
     CDS             complement(64..606)
                     /locus_tag="U4B29_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN2107026.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="U4B29_002086"
     CDS             741..1130
                     /locus_tag="U4B29_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107027.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="U4B29_002087"
     CDS             1134..1898
                     /locus_tag="U4B29_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2107028.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="U4B29_002088"
     CDS             2027..2974
                     /locus_tag="U4B29_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2107029.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="U4B29_002089"
     CDS             3104..3844
                     /locus_tag="U4B29_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107030.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4135..4803)
                     /locus_tag="U4B29_002090"
     CDS             complement(4135..4803)
                     /locus_tag="U4B29_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2107031.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4937..5224
                     /locus_tag="U4B29_002091"
     CDS             4937..5224
                     /locus_tag="U4B29_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107032.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5640..7178
                     /locus_tag="U4B29_002092"
     CDS             5640..7178
                     /locus_tag="U4B29_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN2107033.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7175..8140
                     /locus_tag="U4B29_002093"
     CDS             7175..8140
                     /locus_tag="U4B29_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107034.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8127..9449
                     /locus_tag="U4B29_002094"
     CDS             8127..9449
                     /locus_tag="U4B29_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2107035.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9512..9616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9690..10433
                     /locus_tag="U4B29_002095"
     CDS             9690..10433
                     /locus_tag="U4B29_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107036.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10448..11440
                     /locus_tag="U4B29_002096"
     CDS             10448..11440
                     /locus_tag="U4B29_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2107037.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11452..12189
                     /locus_tag="U4B29_002097"
     CDS             11452..12189
                     /locus_tag="U4B29_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107038.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12179..12901
                     /locus_tag="U4B29_002098"
     CDS             12179..12901
                     /locus_tag="U4B29_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2107039.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12985..>13404)
                     /locus_tag="U4B29_002099"
     CDS             complement(12985..>13404)
                     /locus_tag="U4B29_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2107040.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4416 a         2294 c         2786 g         3908 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg
     3961 aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc
     4021 agatcgtttg caaaaatagt ttattttttt ataaagaaga ttttactgaa taatatcatt
     4081 ttagtttaca taatgatatt tctgtaaatt aaataaagta gtgaaaaata agaatcagtg
     4141 ctctaactgt tcaagaggat caagtggttc aaatgtttct gtagtctgtt ttgcttccaa
     4201 ttggggcaaa acagatccaa tttccagata cttcacttta ttcaatgtat aagatagaat
     4261 agaacagcta agtaatgtat acagaaaatg tgtgagatcc aaataaagca aggagacaac
     4321 taaaatacca aaagtaatca agaaaagact tttgccaatc ttaatatgaa aatatttttc
     4381 aatggcttga taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa agacgagacc
     4441 gcaaccaatg ccagaaagaa tacctgtcat caaactattg atcagtagac tatctacagc
     4501 aaaatgtata tgaaggctct cccataaatg taaaaagaaa actagcgcaa atgttccgac
     4561 gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat gagataagca ataaaggtaa
     4621 attaaaaagc agtgaagtga cagaaggagc gaatccaaaa agattatgca aaagcaaagc
     4681 aataccagta accccaccat ctgccagctt agatggaata ttgattcctg tcacagctaa
     4741 tccgtagatt cctgcgccta aaatgattcc gcacaattct gtaaataaat ttaatttttt
     4801 catttctagc ctcttttctt ttgaaaattt tcagcaactt ctttattata ctgacttata
     4861 ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt ttatttatca
     4921 aataaggagt ggaaaaatgc gagcagcaga cttattgaca attcttacaa aatcacccaa
     4981 aaaaacagag tatcaaggag tttttttggc tcaagaacaa aaactggtgc ctgtgacaat
     5041 ggtccagata aataaagcaa atcaatttat ctttttattt gaacacggca aagcaccact
     5101 tgctatgaaa gacatctttc tttttttgat gaagaaccgt gagaaagctg tttattatca
     5161 gtcaaaagaa gaactcattc ctctttatgg agtgaaagag atagaaaata aagtggtgat
     5221 ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa acaaccattt tttacatttt
     5281 tggcaaaaaa gtgtcatgat acagtataat attcgttgaa caggccaata aaggtgttct
     5341 cccccacgag atttctactt tattggctgt ttctttttat taaaataaaa gttgtgtgtg
     5401 taaaaaagat atagctgatt ccaagtgaca atttcgtata gatttttatg gtattcgtag
     5461 tgaaaaaacg agaaccaaaa agttgatccg actttgttgc ttggattctt tttttgtcag
     5521 catatttaga ggtatttttt tctttttttt gttcgtgctg taaggttaag gcaagacaag
     5581 ttagttggga aaaaggaaag aaatgtttat acttaaagaa taacagaaag gaaggtcgca
     5641 tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa gctgcatttc
     5701 aacatctgtc catttctctc atttctttgc tgattgcagc actgattgct attcctttag
     5761 cgatttgggc agccaatcat aaaaaaatgg cagaagtctt attgcagatc acgggtgtgc
     5821 tccaaacgat tccttcactt gccttattag gtttacttat cccatttgta gggatcggaa
     5881 ctgttccagc attgattgca cttgttattt acgctttgct tccgattttc caaaacacgt
     5941 atattggtct agctgaaatt gacccgtcta ttgaagaagc agcggtagca tttggtatgt
     6001 ccagaatgag acgcttgctg aaagtagagc ttcccatcgc attacctgtc attatctcag
     6061 gtatccgaac tgccttagtt ttaatcatcg gtacggctac tttggcagca ttgatcggag
     6121 caggtggatt agggacattt attttgttag gtattgaccg aaatacacct gttttgacat
     6181 tgattggtgc gatcagttct gccttgcttg caattatatt cagcggactg attcgctggt
     6241 tgcagcataa gaaacctcga tattccatca ttactttagc agcagtggtg tgtttatttg
     6301 gcggcgtttc tttatatcag tctgccttgt ttaaaaatga aaccatcacg attgctggaa
     6361 aattaggagc ggaacctgaa atcctgatcc aaatgtataa agaattgatt gaagacaaca
     6421 caaaaacaga agttgaatta aaaccgaatt ttggcaagac gagttttttg ttcaatgcac
     6481 tggatcaagg tcaaatcgat atctatcctg agttcacagg tactgtccta gaaagcttgg
     6541 tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca tatgacgaag
     6601 cagaagaatt attgaataag caattcgata tgacattatt aaaaccaatg gcttaccaaa
     6661 atacgtatgc cttggcagtc aagagatctt ttgcgaacga acatcaactt aaaaagattt
     6721 ccgatttggc agctatctct gatcagatca aagcgggatt cacactggaa tttattgatc
     6781 gttcagatgg atataaagga atccaagaag catatggctt gaatttttca agtgtacaaa
     6841 gcatggagcc aagtttgcgt taccaagcaa tcaataatgg agatgtcaat attgtggatg
     6901 cctattccac agatagccag ctcaaggagt acgatctagt gactctagaa gatgatcgag
     6961 ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct aaagaacatc
     7021 ctgaaatcgt taaagcactg aataaattaa gcggaaaaat aacagaggat caaatgattg
     7081 atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca cgaatcttcc
     7141 tgaaaaatga aggattagta aaggaggaag atcagtgact aaattaatcg agttcaaaca
     7201 tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac ctaaatctgg caatcaaaaa
     7261 aggagaaatc tttgttttgg ttggtccttc cggtagtgga aaaacaacta ctttgaaaat
     7321 gatcaatgga ttatctaaac catcagcagg agacatctac ttcaaaggaa aatcgttaaa
     7381 tgaatataat ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc aaatcgcttt
     7441 gttcccgacc atgacagtca agcaaaatat tgaagtcatc cctgaaatgt taggttggga
     7501 aaagcagaaa agagcagacc gtgtagatga acttcttcaa aaagttggct tatctcctga
     7561 tatttatcga gatcgtatgc ctagagagtt gtctggagga gaacagcaac ggatcgggat
     7621 catccgagct attgctgcaa gcccagatgt gattttgatg gacgagccat ttagcgcatt
     7681 agatccgatt tcaagaaact cattacaaga attggttttg tccttacacg aagaattggg
     7741 cacgaccatc gttttcgtta cgcacaatat ggaagaagca atcaagctag gtgatcgtat
     7801 tgcatttatg aaggatggag aaattatcca atgtgacaca ccagagcagt tattgatgaa
     7861 tcctaaaaac gactacgttc ggcatttctt tgatgaaccg aaacagacga aagagtggcg
     7921 agtagaagat ttagtcgtca atggatattt cttaaatgaa ataccagaaa atgctcgtca
     7981 acaagtttgc tttgatacgc ctatgaaaga agtatattcg cttctttcgg tgtatccaag
     8041 cattgtgata gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag aaatctttgc
     8101 ttttttaagt cgagaggagg aaggaaatga aaacatatga tgcaatcatc atcggttcag
     8161 gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc aggtaaaaca gtactagtgg
     8221 tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg atgtgatcct aaaaaagtct
     8281 tgttaagtgc agtagaggca cgtaatcggg taaaacaatt gagtggaaaa ggttttaacg
     8341 agatcccaac tgccaactgg gaagaacttc aaaaattcaa acgaacattt acggatccag
     8401 tcccagaaag ccgaaaaaaa caattagcag aagctgaaat cgatcatctt tcaggaaccg
     8461 ctcgtttttt ggatgattca tctattgaag tcaacgaaga agtcttccat gcggattact
     8521 tagttttggc aaccgggcaa agacctacga tcttaccagt ggagggaaag gaatacttga
     8581 aaactagtgc agacttttta tcattgcctg tactgcctaa agaaatcatt tttatcggtg
     8641 gtggttatat tgcttttgag ctagctacga ttgccaatgc tgcagggagc aaagtaacca
     8701 ttgtccatca taatcaacgg ccattaaaag aatttgaggc gtctttggta gaggaagccg
     8761 tccatcaaat ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc caaaagatca
     8821 tttcagaagg tactcgctat cgtttagtcg gaaaagagac ggaattagta gcagatatga
     8881 ttttttgtgc gactgggaga cagccgaata cagaatcttt agcacttgag caagcgaata
     8941 tcgtctttga taaacacgga attgcagtca atgattatct gcaaacgagc aatccaaaaa
     9001 tttttgcgtg tggagatatt gtttcaagga aaaccccaaa attgacacca gtcgccactt
     9061 ttgaaggaaa ttatgtagct aagcggataa cggatgcaac aagcgaacct atcaaatatc
     9121 cgattatacc tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga gttacaaaaa
     9181 gtcacgcctc ttcgtcagat caagtggtag agatggatct taccagttgg ttcacctatc
     9241 atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt tgaccagcaa aattatttga
     9301 ttggtgctgc agtgattagc gagcaagcag acgaattgat cgatgatttg acactcgtca
     9361 tcaatcaaaa attgactaaa aaagagttgg atagctatat catggggtat ccgacattgg
     9421 caagtgatct ctcctattta ttgaaataat cagcatgata actaaagcgc ttgttgcttt
     9481 ttccttcttg tgctacactt taaaaaatga atacctgttt ctggggatgt cgtagacgac
     9541 atgagagtaa gttgtgaact tagacccatt gaacctgtca gttaatactg gtgaagggag
     9601 aaacatagca gacgcttgaa gcgggttact atgtcgtgcc catttcaagc gttttttgtt
     9661 attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca gcagtcagta
     9721 tggcattcat tttaattatt tggcagattg ttgtcatagt ttggaaaata cctgccttta
     9781 ttttacccgg acctgctgct gtattttctg cattagcggc agatgcacaa gtattacttt
     9841 cccattctgt cacgacatta aatgaagcag tgcttggttt ggtaatagct agcgttcttt
     9901 cttttattac tgccttggca atggattatt ggaagttttt agaaatgtct ttttatccgt
     9961 tactagtgat ttcacaaatg ttgccaatca tggtgttagg gccattactt accctatggt
    10021 ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt gataagctat tttccgatcg
    10081 tagtttcttt ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc tttttgaaaa
    10141 cgatgggggc agatacaggg aagatttatc gaatatataa aataccagcg ggtttatccg
    10201 gcttcttttc cggtttaaaa gtggctgcta cttattgtgt cagcggagca gtcgttggag
    10261 aatggctaag cgctcaagca ggcttagggt attacatgat acgcgtaaaa aacagctacc
    10321 agatcgataa agtttttgct gcgatcattt gtgtcattct attgagttta ttgctgaatg
    10381 gcgcttgttc attattgaaa aaagggtatt atcgtatgct ttatattttt taagagaagg
    10441 ggatcaaatg aaaaaattat ggcttttatt accgctactg ttgcttttat ccgcttgtgg
    10501 cactgcaaaa gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag tgacacttgt
    10561 tttggattat gtaccgaata ctaatcatac tggcatctac ctagcaaaag aaaaaggcta
    10621 ttataaagag gctggtttaa atgtccaaat catcgaacca ggagataatt cgacaagtat
    10681 tggtctagta ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag atgtcactta
    10741 tgctcacgca gatggacaga atataccagt caaagcaatt gctacagtga tcaaacataa
    10801 tacttctgga ttcgccaccc tatcagacag taacatccat tcaccaaaag attttgaagg
    10861 aaaaacatat gcaggttggc aaagtccaag tgaagaagct gtcttaaaag ctgtgatgga
    10921 aaaagacggc ggggactttt caaaactgac aatggtagga agcaatggag aagggccaga
    10981 aagcttagga aaatcctcag atatccaatg gtactttgaa ggttgggata tgatcaaagc
    11041 aaaagaagcg ggtattgaac taaactatat cccattgaaa gaattagatg aacggttaga
    11101 ttactatact ccggtaatta ttacgaatga tcaactgatc aaaagtgatc cagaattggt
    11161 acagtctttt atggatgcca cgaaaaaagg ctatcaggaa gctattaagg atccaaatga
    11221 cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt tagaagaatc
    11281 acaagcattc ttgtcaaaaa attatacgga tgatcctgag aattggggat tgatggaaga
    11341 aaaagtttgg cgcaattata cagcttttat gcaggaaaac ggattgatca agcaagatgt
    11401 accttcgcaa gaactattca ctaatcagtt tataaaatag gagtgataga tatgtcaatc
    11461 caaatcagcc acttgaatgt agtcattgaa aacctgaaaa tattagaaga catccatttc
    11521 actatcccta aagatacctt tacatcgatt gtcgcgccaa gcggagccgg taagtcaaca
    11581 ttgctgaaaa ctttaacagg agtccagcct atgacttctg gaacgatcaa agtagacgat
    11641 caaaaagtaa ctggtctcca tacagcattt agttatatgc cgcaagaaga tatgttactt
    11701 ccttggctga gcgtctatca aaacgttacg ttatatcaaa aaatcaatca tctaacaatc
    11761 aatgaagaac aagtaaggga ttatctggat atctttgggt taaacggcta tgaacatttt
    11821 ttgccggaac aattatctgg cgggatgaag cagcgaacgg ccttgttacg aacaatcatg
    11881 aatccttctt cttacttgct tcttgatgaa ccatttggag cgttagatgc tatgaccaga
    11941 gggcagatgc aagactggat gttgaagctg cctaaaaaag caaagcggac tactttactt
    12001 gtgacgcatg atatcgaaga agcaatttat ttatcagaca ggattcttgt gctttccgcc
    12061 cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc acgtgaatgg
    12121 ctactgcagc aatcagagtt gaagcaaacg atttatcagt tattggcagg tgaattagat
    12181 gttaagtgat gctcattgtc atttagatgg gagtcagagt ttagcactcc ttcagcagga
    12241 acacacgatt ttgacgatca tcaattgtga ttcgccagaa gaatggaagg aaaacagaca
    12301 gcttgcagca agtaagactc aagccttaag ttacggtatc catccttgga aagcagatag
    12361 ctatacgttt gaacaagttg agcctttttt gaaaaaagca aggatcatcg gtgaaatagg
    12421 gttagataat atttggacga atgtaccgat gactacacaa aaaaaagtat ttgagcgaca
    12481 gcttgctttt gcagccataa atgagaaacc tgttgtactg cacacaaaag gatgcgaaaa
    12541 agagatctta aattatatcc aagcttaccc taatcactat ttgatccatt ggtattcttc
    12601 tttggactat caacaagact atattgattt aggctgttat ttcagcattg gtcttgattt
    12661 gaaaaagaat ccagcagtct ggcaactagc taaagcagtc ccaatcgacc acttacttat
    12721 tgaaacggat ggactacatg cagcagagtg ggctttaggg gggatttttt cagaaaaaga
    12781 ttatgtctca ctgcttgaag aacagttaaa gacaatcgct gagatcaaag agctatcagt
    12841 tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt ttcattagat taggtaaata
    12901 aaaaagagat gtgacacaac tagtgtcgca cctctttttg gctttctttg tataaaagta
    12961 aactgtttga ttaataaatg aatcttagta ccaaccgttt gcttcccaga atgctttcgc
    13021 cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt cttggtttgc
    13081 agcagagtag tcaccattta agtaagaaga atctaattgg taacgtccga tgtaacgtcc
    13141 gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt ttgcagagct
    13201 tgtgcttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg tttcagcaac
    13261 aggcgctgct tgtttttccg tttgaaccgt ttcttgtaca acaggttgtt cagcttggac
    13321 tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc cttctgttgg
    13381 gattgttaat tgttgtcctg agta
//
LOCUS       DASJTM010000074        13172 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJTM010000074 DASJTM010000000
VERSION     DASJTM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.074"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            139..1878
                     /gene="yfcC"
                     /locus_tag="U4B29_002100"
     CDS             139..1878
                     /gene="yfcC"
                     /locus_tag="U4B29_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2107041.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2035..3219
                     /gene="iadA"
                     /locus_tag="U4B29_002101"
     CDS             2035..3219
                     /gene="iadA"
                     /locus_tag="U4B29_002101"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN2107042.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3330..3533
                     /locus_tag="U4B29_002102"
     CDS             3330..3533
                     /locus_tag="U4B29_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2107043.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3634..4296)
                     /locus_tag="U4B29_002103"
     CDS             complement(3634..4296)
                     /locus_tag="U4B29_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107044.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4309..5811)
                     /locus_tag="U4B29_002104"
     CDS             complement(4309..5811)
                     /locus_tag="U4B29_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107045.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6359..8038
                     /locus_tag="U4B29_002105"
     CDS             6359..8038
                     /locus_tag="U4B29_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2107046.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8165..8644
                     /locus_tag="U4B29_002106"
     CDS             8165..8644
                     /locus_tag="U4B29_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN2107047.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8863..9543
                     /locus_tag="U4B29_002107"
     CDS             8863..9543
                     /locus_tag="U4B29_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN2107048.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9551..10882
                     /locus_tag="U4B29_002108"
     CDS             9551..10882
                     /locus_tag="U4B29_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2107049.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10897..11859
                     /locus_tag="U4B29_002109"
     CDS             10897..11859
                     /locus_tag="U4B29_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN2107050.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11970..12308
                     /locus_tag="U4B29_002110"
     CDS             11970..12308
                     /locus_tag="U4B29_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN2107051.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12387..13094
                     /locus_tag="U4B29_002111"
     CDS             12387..13094
                     /locus_tag="U4B29_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN2107052.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4194 a         2322 c         2887 g         3769 t
ORIGIN      
        1 ggagaaaaaa aacaaaatag aaaaaagtga ttttctaata aattattcat gtggaaatgt
       61 catttttact gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa
      121 agaaaggaag aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc
      181 actaaattat cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg
      241 atactgactt ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat
      301 gcaaatggcg aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac
      361 gagctggata agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa
      421 cgtgaaaaac tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa
      481 aatcttacgc aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag
      541 tacggcgaaa atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga
      601 acagatgatt ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga
      661 gataagtatg gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt
      721 ggtattatta tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact
      781 aagggactgg agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct
      841 acattcggga tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt
      901 gcgttaggat atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga
      961 aatgcgacct cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc
     1021 cccgtgttgt ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc
     1081 gctggttata tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact
     1141 tacaaatcag atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa
     1201 ttcatgcttg ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt
     1261 tggggcgtga ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg
     1321 ggattagttg ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac
     1381 atcgcttctt ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt
     1441 atggccaaag ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg
     1501 aatacgattt tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct
     1561 tgggcgatgt acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa
     1621 gcagctttga ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa
     1681 gtagcagttc ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca
     1741 gccagcttga ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc
     1801 caaatcaaga tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt
     1861 gcaataggtc tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat
     1921 aaggactcca agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg
     1981 gattattttt taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa
     2041 atcattcgac aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt
     2101 gccggtggga agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa
     2161 gtagaagaac tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc
     2221 catattttag gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg
     2281 agccaattga ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa
     2341 ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc
     2401 acttatgtat acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc
     2461 aaagactttt tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac
     2521 cgttcttctc agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga
     2581 ggcatgttat ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag
     2641 ctagagctat tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct
     2701 actcatgcaa atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt
     2761 ggcacgattg acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa
     2821 gttcgcttca gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc
     2881 acgatgacct cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta
     2941 gggttaggag taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa
     3001 gaaagtatcc ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg
     3061 aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat
     3121 aaagaaacgc ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa
     3181 gaagtcaaag tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt
     3241 tttagaaaac gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat
     3301 caataaacaa actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata
     3361 tgaaagataa agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga
     3421 gcaaagaaac agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa
     3481 atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac
     3541 ttattactaa ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg
     3601 ttcggatttt ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt
     3661 attttttagt tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat
     3721 gataatacag cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga
     3781 agttgtttca tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca
     3841 gatagcacga acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt
     3901 ttttgctaat atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata
     3961 attctcttgc ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa
     4021 ataaggaagc aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct
     4081 taacccgatt ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc
     4141 tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc
     4201 gtacattttt ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc
     4261 ttgataccaa aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag
     4321 gatttttctt gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact
     4381 gattccaaat gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa
     4441 ttcgctgact tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg
     4501 ttgaccgcct tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt
     4561 ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac
     4621 gtttccgcta agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa
     4681 caattggaga atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg
     4741 ccgacgctcc cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagc
     4801 cactagtaaa acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga
     4861 ctgatacatg gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc
     4921 ttctttgaga aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat
     4981 cgtgttatcg tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt
     5041 attcgacgga ttcatgaagt tgaatttcat acccatactg ccgcttgttt ctctggattc
     5101 ataaattcct ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt
     5161 gaaagtatct ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt
     5221 gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc
     5281 agaaaaatta ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt
     5341 catttgcgga ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga
     5401 actgtcttca cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga
     5461 ggcggaactt tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc
     5521 cgttaaggaa actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga
     5581 accgcttgaa gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt
     5641 cactgttgct ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat
     5701 cgtgaggcca gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga
     5761 acgtccccat tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat
     5821 ccctcctagt agatgcattt gataatagac aacctatctt aaagctagct taaatcttat
     5881 gctgtttgca caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc
     5941 aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg
     6001 aatagcaaaa gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca
     6061 attgctgata ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact
     6121 tatataagta ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc
     6181 caaaataaaa tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat
     6241 tctccataag ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt
     6301 agataaagaa gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt
     6361 gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt
     6421 cgcagaagtg ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga
     6481 gttgttgggg atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg
     6541 tgtagctggt gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt
     6601 gttgtcaaaa atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt
     6661 gaatgtcagt cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca
     6721 tacagaaata gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc
     6781 agattcagtg gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt
     6841 taaatttgac caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat
     6901 cggcaaagaa ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc
     6961 tgtagcttct gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg
     7021 gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc
     7081 agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg
     7141 cacagcgatg cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa
     7201 agacatgaaa aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga
     7261 acccatcaag tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga
     7321 aggagatttg gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa
     7381 aacggaagat atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg
     7441 cgtttctggc catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac
     7501 atattttgtg ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca
     7561 tgcagtaggg atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata
     7621 taagaacaag cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg
     7681 attaggcata ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga
     7741 cgggattttc gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc
     7801 tcaaattact tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag
     7861 cagcaatatg attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag
     7921 caaattaaag caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg
     7981 ccgcccagtg atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac
     8041 aaaaagagta agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac
     8101 aaataatttt tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga
     8161 atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca
     8221 accacttgat tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg
     8281 gattgatcca gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat
     8341 catcgcattg attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa
     8401 agatcgtaaa gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca
     8461 cggagcaaac acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga
     8521 cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct
     8581 tcggataaaa gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag
     8641 ttaagggacg agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc
     8701 ttggtacttg gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca
     8761 atccaatgaa aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg
     8821 tttttaataa aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat
     8881 ggacattcct gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt
     8941 attacaggaa atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg
     9001 attactcatg gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa
     9061 gccacaatta tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca
     9121 ttcggtatga atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat
     9181 gcacagcata gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc
     9241 ggaattatta ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc
     9301 agtgatctag gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc
     9361 gataactata cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag
     9421 aaagtagttc caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt
     9481 gtagagttac tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg
     9541 taggcagaaa atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt
     9601 tggcaatcga atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc
     9661 gtaccgtgca atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg
     9721 gaccatccgt atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt
     9781 cgtcaggatc attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa
     9841 taaattcgtc atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc
     9901 actaatgatc gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc
     9961 agtattgatc acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct
    10021 tgaaatgccg atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg
    10081 agcgttaagc gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc
    10141 agctgaaaag actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc
    10201 agaagaaacg cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat
    10261 tgttacggca aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac
    10321 gaaagaacca atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat
    10381 ctgggatggg ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat
    10441 tacccggcaa gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga
    10501 tacgatttcg gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa
    10561 tcgtttggaa aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg
    10621 aaccatttct tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt
    10681 gatgaaccaa aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat
    10741 ccaattggaa agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc
    10801 taaactggat atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg
    10861 tcaagtaatg gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta
    10921 gatacaataa aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat
    10981 gcattaggat cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa
    11041 atacgtcaag taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt
    11101 gaagatgtct tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga
    11161 atcagtgatc aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat
    11221 gatgaccctt atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg
    11281 attgcagaat ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg
    11341 ttactttatg caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc
    11401 cgtacattag agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac
    11461 agagagattg aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat
    11521 atccaggtgg atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa
    11581 tatggaatcg atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa
    11641 gtgctggctt gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt
    11701 tctaaggggc cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta
    11761 gcaagtggtg cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa
    11821 gatatatgcc aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc
    11881 ttttgtagta aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg
    11941 ctcactaaag attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct
    12001 cagagtacac atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga
    12061 acaaagaaga acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa
    12121 accgtctgtt agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt
    12181 caggtgccat taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata
    12241 atatcgattg caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa
    12301 agaattaaag aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa
    12361 agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc
    12421 cgacatttct tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca
    12481 atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag
    12541 cagcacaata tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg
    12601 gagatctcac agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg
    12661 cacagtctac ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac
    12721 aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact
    12781 ttggttcgat gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta
    12841 cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat
    12901 taccagtcga atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac
    12961 tgacggaaga agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg
    13021 ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca
    13081 accgaagaga ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga
    13141 gagttagctg attgaatacc atttatttgg aa
//
LOCUS       DASJTM010000075        13043 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJTM010000075 DASJTM010000000
VERSION     DASJTM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.075"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..686
                     /locus_tag="U4B29_002112"
                     /pseudo
     CDS             78..686
                     /locus_tag="U4B29_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="U4B29_002113"
     CDS             706..1734
                     /locus_tag="U4B29_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2107053.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="U4B29_002114"
     CDS             1731..2489
                     /locus_tag="U4B29_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2107054.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="U4B29_002115"
     CDS             complement(2575..3315)
                     /locus_tag="U4B29_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2107055.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="U4B29_002116"
     CDS             3376..4629
                     /locus_tag="U4B29_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2107056.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="U4B29_002117"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="U4B29_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="U4B29_002118"
     CDS             5300..5626
                     /locus_tag="U4B29_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2107057.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="U4B29_002119"
     CDS             5694..6407
                     /locus_tag="U4B29_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2107058.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="U4B29_002120"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="U4B29_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="U4B29_002121"
     CDS             7690..9555
                     /locus_tag="U4B29_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2107059.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="U4B29_002122"
     CDS             9603..11012
                     /locus_tag="U4B29_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN2107060.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="U4B29_002123"
     CDS             11120..11821
                     /locus_tag="U4B29_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107061.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="U4B29_002124"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="U4B29_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4377 a         2191 c         2756 g         3719 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agt
//
LOCUS       DASJTM010000076        13012 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJTM010000076 DASJTM010000000
VERSION     DASJTM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.076"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(118..1368)
                     /locus_tag="U4B29_002125"
     CDS             complement(118..1368)
                     /locus_tag="U4B29_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2107062.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            1778..2077
                     /locus_tag="U4B29_002126"
     CDS             1778..2077
                     /locus_tag="U4B29_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107063.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            2150..3511
                     /locus_tag="U4B29_002127"
     CDS             2150..3511
                     /locus_tag="U4B29_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN2107064.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            3533..3793
                     /locus_tag="U4B29_002128"
     CDS             3533..3793
                     /locus_tag="U4B29_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107065.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            3807..4559
                     /locus_tag="U4B29_002129"
     CDS             3807..4559
                     /locus_tag="U4B29_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN2107066.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(4653..5258)
                     /locus_tag="U4B29_002130"
     CDS             complement(4653..5258)
                     /locus_tag="U4B29_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2107067.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(5311..6285)
                     /locus_tag="U4B29_002131"
     CDS             complement(5311..6285)
                     /locus_tag="U4B29_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN2107068.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(6663..7382)
                     /locus_tag="U4B29_002132"
     CDS             complement(6663..7382)
                     /locus_tag="U4B29_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107069.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(7509..8780)
                     /gene="celB"
                     /locus_tag="U4B29_002133"
     CDS             complement(7509..8780)
                     /gene="celB"
                     /locus_tag="U4B29_002133"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2107070.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            8933..10330
                     /locus_tag="U4B29_002134"
     CDS             8933..10330
                     /locus_tag="U4B29_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2107071.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            10411..10851
                     /locus_tag="U4B29_002135"
     CDS             10411..10851
                     /locus_tag="U4B29_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2107072.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            10999..11325
                     /locus_tag="U4B29_002136"
     CDS             10999..11325
                     /locus_tag="U4B29_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2107073.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            11326..12075
                     /locus_tag="U4B29_002137"
     CDS             11326..12075
                     /locus_tag="U4B29_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107074.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(12155..12610)
                     /locus_tag="U4B29_002138"
     CDS             complement(12155..12610)
                     /locus_tag="U4B29_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2107075.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         4101 a         2272 c         2646 g         3993 t
ORIGIN      
        1 agatgaaatt taagattttc tttattgagt aatatgtttt gaagcgcacg aaactagacc
       61 cgatcaaaat caaatttttt tcgggttgat tcaaggattt gcgtcaataa cggattgtta
      121 tttctctatc acactccttg gtggcgtata gagttggtgt ttacgtagta gcgtatccac
      181 cagacgcgta aattttcttg cggttaggac gagtgcacgt ttgtgttgat gctttggaac
      241 ctccttatat ttactttgat aaaaagcttt gtattcagga agataatttt ttacagagtt
      301 ggcagcttca actaagtagt atctaaaata tcgatttcct tgttttgtca gaggtgtatt
      361 ttgcgactga tagtttccag attggttcac tttccaactt aatccagcgt attttgccaa
      421 ttttgtctga tcttcaaatc tttcgatttg accaatttca gcgattaaac cggcagcata
      481 gactttacca acgcctggaa tactggttaa gcattgatat tctggaatga caacaattac
      541 ttgttcgatg gctttatcta attctttgat atttttttca agtgctcgta tttctcgtac
      601 gagaacactt aaaacaacat taattgattc ttgtgcaaga gcacctaggc ggtaactcat
      661 ggtaattgca cgttgaattg ctttagcgat tttttctggt tgcttgaaac ggccttttcc
      721 tttttcttga agtaaatcac agaaagcttc taaaggaagc tcagcaagtt catccaatgt
      781 gaaatcttcc gtcatgagtg aaatgagggt tgcactaaaa agactcgtcg attcgtctcg
      841 catttctcga gcaagcgtat tacatttgta cgttagattt tcaagaaaat gttgctttgt
      901 acgaatcaat tgcttaataa gttgatagcg agtacgtgtt aaacgctgta aggccatgta
      961 tttctcttct ttgatagggg aagttgtgaa tcgttgaata cgcaagaaat cagcgattct
     1021 taatgcatcg tttcgatcag ttttattctc atcaaacatg cgactaaatt gtttcacccg
     1081 aaaaggattt tcaatcgtta caagtgtgtt gagcttcttt agtttctcat cttcatgaaa
     1141 aaacatggaa ggatggaagc tgtacatgga cgttgactcc atgccaatca caatttgatc
     1201 aaagtggtag ctgtcattga attcaagaat tgtttctcgt atgaatgaag caccttcgat
     1261 gtcattggca acagagattt cagataagat agacaattga ttgtcgtctg ttaaaaaaca
     1321 aacatctagt ttttcagagc taacgtcaat accgacaaat aatttcatgg aagaaggacc
     1381 tcctttcgat ataaattttg gaaaatgctt cgatactgtg ggatttccca gaaactcact
     1441 gtatagccac aacctcgcct aatagaatac ggtgataaaa gagctagaag ctgccttcca
     1501 aactactatt tgaaagtcta gtccattatc gaaacagcta atgagttggt agtgataaca
     1561 gtggttcggg taacagacta aacagggtag acaaacgcta caggaggaaa tagcaaattc
     1621 ccgttggatc ctttccatac atccattgta ctctgagaaa tcacacaata tcgaaacaaa
     1681 ttccgttagg aatgtgttat tcaataaatt ttataatcta gaaacatttt gaataaaaac
     1741 tattagacaa atgatatttt acgaggagga agaaaaaatg aaaaatcggt taattgtcgc
     1801 atgtggaagt ggagtagcaa cgagtcagac aatcgctagt aagatccaaa gtatgttaga
     1861 agatgacggc atcgcatttc ctgtagaagc agttgattac aaatcgattc aaaatgagct
     1921 tctaactgct ggaatctatg tgtatgtcgc acaacctgat gaggaagtac ttgaacaagc
     1981 aaaagatttg ggtattgaag tatttccagg aattccattt ttaactggaa tgggtgtaga
     2041 acctatttat gacagtatca aagagttagt acagtaaagg aggattgttg gataaaaaag
     2101 ttggttcata gttttattta ctatatttag aaagaaagaa ggtttattta tggaagtttt
     2161 ccaaaccatt atcaattatg ttttgaactt aggatcagcc atttttgttc ccctgattat
     2221 tttgatactt ggattgttgg cagggatgaa attaaaaaaa gcattcatgt ctgctttgac
     2281 tttagggatt gcttttagcg gaatgtctat ggtcattggc tatatgtcta acgcagtaag
     2341 tccagcatca gaagcattgg ctaaaaatac gggaatcagt ttgcctgcac ttgatcttgg
     2401 gtggacaggt gcagcaagca tcacatggtc gtggagttat gcttttatat tttttgcagt
     2461 aacgattggg gtcaattttg tactgttact ttttaattgg acgaagacgt taaatgttga
     2521 tatgtggaat gtttggggga aagctttaac tgcttattta gtttattatg tttccggtag
     2581 tttggctgca ggctttctta ctgcaatggt ccaagttata ttggaattaa agcttggtga
     2641 tatgtttcaa aaacatatac aagatctcac agggattcca ttagtaacag tgactcattt
     2701 tatgacatct gccgctgtct tgttgctgcc tttcaatatg atcatggata aaattcctgc
     2761 tttaaataaa cgagcggata cgaatgcatt gaagaagaaa attgggatct tctctgaaaa
     2821 ctctgtaatg ggattcatta ttggattggg gcttggcttt gctgctgcgt atggcgtttc
     2881 aggttctctt aatctagcga tccaaacagc tacagcatta agtttgtttc ctatgatctc
     2941 taaaatgttc atgcaatctt tgtctcctct agcagacgct atgtcagaat ttatgaaaaa
     3001 gcggtttaag gatcgtgaag tatatatcgg attggactgg ccgatattag ctggtcgttc
     3061 agaaatttgg gttactgcta ttttacttgt tcctgtgttt attggttatg ccattatttt
     3121 gccaggaaac caagtattgc cattagcagg gattatcaac tattcgattg cggtcggcgg
     3181 attactttta acaggaggaa acttggctcg tatgctgatc ttgggaatca tcactatgcc
     3241 tatttatttg tacggggcca cttaccttgc gccaatactc agtgatttag cagaaaaaag
     3301 cggagcagtc gagggaatca agaaaggaca gttgatcaca tggtcatcca tagaagggcc
     3361 ggagttcagg gttttatttg cggaggcatt taataggaat attctcgcta ttgtaggatg
     3421 cgtgattttt ctaggactgt ttgttctact ttataaatat atgataaaag cgaaagtacc
     3481 gagtgaacgt tatgctgaac tacaaaaata gcattgaaat ggtgggaaag taatgaaaag
     3541 agaagagaca gataaaataa aatggacagt cgctctttgt ggtacacttc tgctatttct
     3601 atatggactc tttactcaga atatcatcat caatctattg gtcatttttt tcgcactagt
     3661 gatctataaa tatggaaatc acgttttatt cagagagtac gatgaaaaaa gaaaacggaa
     3721 aatagaagaa tcaatgaaaa taaaagaagc taccaaagaa atattgagag aaaaaagttt
     3781 tataaaaaga taatggaggg gtcattatgt cagatggacg tatgttattt gagagagaac
     3841 gacaggacat ggcacgtatc gtaagagtga ttttcgaaag gaaaaacaca aatgtcgcag
     3901 gagggaattt ttcctttaaa accactgatg atcaagggaa agactacatc atcatgacgc
     3961 cgaccatgat gtcagaagcc tatctaggag agatttctcc tgcacaggtt ctagtagtag
     4021 aaccaagaac gcgtagaatc gttgacggag tcggtcattt aactcgtgaa atcaatatgc
     4081 atgaagcgat gtatgagact aatccagcta tcaaagctgt cctacatgct catgcgccta
     4141 atgcaatgtt ctgggcgact actgggttgc ctatgcctaa tttgacagaa gccacagcta
     4201 aagtaaaaga gattcctgtc atggactttg agcctaattg ctctgaagaa cttgctgaat
     4261 tgacaagcga ctatgtaaaa aaacagcatc tcgaattacc gcatatgttg ctgctcaact
     4321 ctcatggcgt attgatcaac acaggtggag aatcaggaat cgaagctatc cataaagcac
     4381 tgcaaatcct tgatacagtt gaatggaatg cagaaattgc ctacaagcag acgatttttc
     4441 aagcattagg gattactgac ggtttttatt caaaaggtga gaaaatcggg ggattggaag
     4501 agttgaaaca taaaacggct atttataata aatctgttat tgcagctggt ggagactaac
     4561 ctatctgaca caaaaaacta cgactttcac aaaatctgat ccagatcgat atctgcttca
     4621 ggttgtatta tgtaagtcgt agttcttttc ttttatttag ttaagaggtg gtctgtccat
     4681 tccctcgctt gcttgatttc gtccatcgtc aactgatgac ctgttacagt tagctgaata
     4741 gtcacatttg cttggcgtgt ttcgaattga tcggccaatt ctttaaagga agcaggggaa
     4801 acgattggat cgttatcacc ataagaaatc cagacggttt tatcctctaa tgtggaacgg
     4861 ctttgatgaa cacctagaga catcggatgg aaaaacaacc ctttatttag ctcggtctca
     4921 cgctccaata ataaatgagc agcgatgttt gcgccatttg aatagccgac aagcagccag
     4981 tcacttaacg gtatcagttc tttttcgctg atttctttga ttttttcatg gagtttatct
     5041 gtttcttttt ccaaactgtc caggtcgaat tgattcaatc cattacgctt gaaaaaacga
     5101 ttcatcccat cttcttggac cgtgccacga aaagaaagca atgtactgtt tggtgcaaga
     5161 aagtgagcga tatccagtaa agaatgttca tcgccacctg tgccatgaag taagatcaaa
     5221 tgcttgccac ttgttccttt ttcaaaaatg taggtcattt tgttccctct tttctgtttc
     5281 tatagatgca aatgaagtag catgaagaga ttattcacgt tttttatttg catcacttgt
     5341 atcgaaagga cggacataag cttcaatggc ttcacgacgt tcttctaaga atggaggaag
     5401 agatagtttt tctcctgctg attctgctgg ttcatcttca aggaatccag gaccatcagt
     5461 tgctaattca aacagtacgt gatgagcggc taggaagtac tcagattgga aatagaaacg
     5521 atcgacgaaa ccagaatttg gcaattcaat cttattgatt acttgaatcc agtattccaa
     5581 tgcttctttg tctgctacac gcaatgctaa gtgatggata tttccaaatc cttcgattgc
     5641 ttttggcaag tctgtccgat gttcaataat gatgcttgca ccatttcccc cttcaccgat
     5701 ttcaaataag tagaaatctc ctgaatggtc tgtttctttg aatcctaaga ttttttcaag
     5761 aatcaagcgg atatgctcta attgactgac acggacaaag acaggtccaa gtcctgtgat
     5821 cgcaaactct gtcggtacat tgctgttttt ccaaggaatg ccagctgcta caccttgatt
     5881 gttttcgtca gagatcaatt gataatactg attatcgaaa tcttcaaatt cgatatattt
     5941 tttgccgaaa cgttcattga ttgctgaatg agtaacgtca tatttttcaa aacgttccaa
     6001 ccaaaagtct aatgctgcgt cacttggcac acggaaagaa gcacgagaga tcgtatctgt
     6061 tccttttttt ccttttggct gatttggaaa atcaaagaaa gtcatatctg ttcctggcgc
     6121 accagtatcg tctgtaaagt aaagatggta agtttcgatg tcatcttggt taactgtttt
     6181 tttgattaag cgcattccta aaatatctgt aaaaaagcgg tagatttttg tagcgctaga
     6241 tgtcattgct gtgacgtggt gaattcctct gatttgtgta tccatgataa atttcctcct
     6301 gttaacttac aaaaagtaag tagttgattt agacatagta tagagggtgc atctctattt
     6361 tgcaattaat ttgtcttcta aagtatcagg aaggaatcaa gaatggaaaa gaggagggac
     6421 ggatcaataa aataaaaaga cttacgacta gctgtatcga tttcaaaaaa actagcttta
     6481 aaaagctaac tttagaatca ttacgggtgg tcataagtct ttttatgctg agttattttt
     6541 tcatttttga gctaatgctt aggatagaat agaatgttga tttcacgatc tcttcttgtt
     6601 acttgaaact gcttaccact gtcacgatct cttggtaaac ggctttttca caacctcttt
     6661 ggttaagttg aacgcacatc cagcacagaa tagttccgaa catcaaatcg gtttcggctg
     6721 gaagaatatt caatcggctg tccatctttg agatagacga cttgctgaac ttccagtaca
     6781 ggatcgtttt ctgcacagtt caaatattgc tgatcatatt catcactttt atctgcagaa
     6841 atagcacgat aagcccctgc aaaatggaca ttcaattctt gatgaagatg atcatagata
     6901 gaggctaaca aatgttcttt ttttaaaccc ggcacaagat ggactggcat aaaggtatgc
     6961 tctaaaacat atggctcgcc atctaagatc cttaaccggt gaatattgta gacaggttgc
     7021 tcggcatcga tcatcaatcg ttcttggata tattgattag ggaattcaac atcaaaatga
     7081 atgatttgac tcttcaaaga acgatgtctg cgtttcattt ggagactcaa accttcatac
     7141 tcgttagcgg gcgatgtgtc tttttgcata aatggatgat tcaagatttt cgttcctgat
     7201 cctcgctgcg aatagacaag tccttccatt gctaagatat tgatggcttt tttgattgtc
     7261 atccggctcg caccaaattc ttttaccaag tcgacttgat taggcaaaaa actatcgaga
     7321 ggatactctt ttgaacggat acgttctctt aaaatatttg cgatttcttc gtattttggc
     7381 acgttattca cctcttctag agtataacga aagattatgc ttccgacaac tcgaagtagc
     7441 atctggaaca ttgtttattc gaaaaaagag gtcatgacaa gtttttgtca caacccctta
     7501 atgatctatt aagcaatatc taaatctggt tgtttatcta gtactttcag gaatgggtac
     7561 cagataaagg caacgatgac aaagtcgatg acttgcagca taccaccaag ccatgaattt
     7621 gttgctaaga caccgcttgc aatgattggc acagtccatg gaacagagac gccagttggt
     7681 gcagggacaa gtccagcagc cattaccaaa taattgaacg tcgttacgat tagaggtgcc
     7741 acaacccaag gaataaggat tgttgcattc aatacgatcg gtagaccgaa gatcgctggt
     7801 tcatttacat tgaaaatacc aggtgctaaa gctaatcgtc caacatcacg atactgtttc
     7861 tttttaagaa caaaggccat caataaaacg acagctaaag tcattccaga gccaccaata
     7921 ccaacagtga aggtttccat gaatggtttt gtgatgatgt gtggtaattc ttttccatgt
     7981 tgataagctt ctaagttatc aagcatcagt gtattccaaa tcgggtccat tacagagttg
     8041 acgatgattt gtccgtgaag tccgaagaac cataagaatt ggacaaagaa caaagcaatc
     8101 aatgttgcag gtaaactact tcccaaacca gttaatggtt tttggatcac ttcataaacg
     8161 acgtcatgta agttagcatt gaaaatacca gtcatcacag cattgattac taagaaaata
     8221 gttagtgtca ctacagcagg gatcaaagcg gcaaatgatt ttgttactgc aggcggaact
     8281 ccatcaggca ttttgatctg ccagccgcgt ttcgtgattc tgcagtagat ttctccagcc
     8341 aagaaagcag cgatcattcc aatgaacatt ccttttgcgc caagacgatc aagtgaaagc
     8401 acaccagtca catcttcacc gccagcggtt ttcattgcaa aaggcgtaag aatcaagaag
     8461 ctggcaaaag acacggcacc accgaaaata ccttcaacgt cataagattt tgataaataa
     8521 tagccgatac caaaagtaac aaaaaccgac atgatgctca ttgttgcatt ttgcccatta
     8581 ccgaataaat tcgataaagt tccctttgtt gcgtcactga aaaaaggcaa gttattgata
     8641 acgacaacga tcgaaccgaa catcgttaaa gggaaggaaa gcatgaaagc atcccgcagc
     8701 actgtaaggt aacgattttg ccctaataaa ttagctaagg gcatgatttt ctcagaaagc
     8761 ttgtccataa aaccgttcat ttttattcac ctcaaattta tttgcaatcg attacataca
     8821 aagtataacg ccaaaaattt taaatgtcta gtcattttca taaaaagtat tgatttattt
     8881 tttagggttc gttatgatta cggtatcaag aaaaaagaga ggaagtaaaa aaatgaaaac
     8941 ctacacattc ccagaaaatt tttggtgggg ttctgctgct agcggaccgc aaacagaagg
     9001 aagagtaagt gatgatggta aaggagaaaa tatttgggat cattggtatg aattagagcc
     9061 tgaaaaattc ttcgatcaag taggacctga aaaaacttcg caagtttaca caaaatacaa
     9121 agaagatgtt cagttaatga aagaaactgg ccataacaca tttcgtacat ctatccaatg
     9181 gagtcatttg attccagaag ggacaggtga aatcaatcaa aaagctgttt ctttttatcg
     9241 agattatttt actgaattgc tggataatgg tattgaacct tttgtgaacc tttaccattt
     9301 cgatatgcca ctaccactgc aagaaaaagg cggatggtta tcaagagaaa cggtcgacgc
     9361 ttatgtttct tatgcagcag cgtgctttga actgtttgga gatctggtca aaaaatggtt
     9421 tactcataat gaaccaatcg tacctgtaga aggaggctac ttgtatcaat tccactatcc
     9481 aaatcatatc aatatgaaag aagcggtcca agtaggctat catgagaatc tagcaagcgc
     9541 aaaggtaatc aaagcctacc atgaaggcgg acatgatggt gagatcggaa tcatcttgaa
     9601 tctgacacca agctatccga gagatgaaac gaacccagaa gatgtaaaag cggcgaagat
     9661 cgctgacgca ttcttcaacc gatcattcct tgaccctgca gtgaaaggag agtttccaca
     9721 agagttgatc gaaattgtca aagaattggc tataatgcca gagatccatg aaggagacct
     9781 agaactcatc cgtgaaaaca cgattgacct gctaggaatc aattactatc agcctcgtcg
     9841 aatcaaagca aaagaaactc caatcaatca cgaacatagc cccatgccag atgattattt
     9901 tgataattac gacatgccca ataaaaaaat gaatccatat cgtggatggg aaatttacga
     9961 aaaaggaatc tatgatattt taacgaatac aagagaaaac tatggtaata tcaaatgcta
    10021 tatttctgaa aatgggatgg gggtcgaagg agaagaacga tttgttaaca gcgaaggaat
    10081 tattgaagat gattaccgga tcgagtttgt tacggatcat ttgaaatacg tccatcaagc
    10141 aatccaagaa ggaaccaatt gtgtgggtta ccacatgtgg acatgtatgg acaactggtc
    10201 ttggacaaat gcttataaga atcgctatgg ctttatcgct gttgatttgg cacaagacgg
    10261 taaacggacg atcaaaaaat caggtcgctg gtttaaaacg gtctctgaaa aaaacggatt
    10321 tgatgcctga tattttttga taaaacgttc acaaaatgtt tgttctaaca ctgtaatcgt
    10381 gctacactaa cagtgaagga ggcgtttaca atggtagata cttatcgtaa tattttagta
    10441 gcaattgatg gttctgaaaa ggcagaaaaa gcatttcttg aggcaatcac gattgccaaa
    10501 cgaaatcagg caacgttgca catcctgtat gtcaatgaag taacaggcaa ttactttggt
    10561 gattttgcat ttgttacaac aagccttcag gaagaattgg atgaggttgc ggaaaaccag
    10621 atgaaagaac atcgaaactt ggcaattgaa aaaggcctga cagatatcga gacatatgtt
    10681 ctttacggtt atccaaagac actgattgct aatttccatg aatcgaaaga accaatcgat
    10741 ctgattgtca tgggggcgac aggtctgaat gcggtagaac gtgcactagt aggttccact
    10801 acttcctatg tagtcaacca cgcatcatgt aacgtcatgg ttgtaaaata aataaattaa
    10861 gggaacagaa agcaaaaaag cataacgctt cttttgcttt ctgttccttt tatttttttg
    10921 taaaaaatta ctttacctga tgaagtaatc atgctattct aattacaacg acagatggag
    10981 taatatggag gaaagaaaat gatttcaagt gatggaatac gcggatacaa tgatgcgatt
    11041 attttatcga tccttaacaa aaacgattct tatggatatg aaatttctaa aacgattcgt
    11101 gagaggacaa atgattccta tgtgatcaaa gagacaacac tttactccgt ttttgcccgt
    11161 ttagaaaaga atcaactgat tgtttcgtat cctggagaag tgactcatgg aaaaaagcgg
    11221 acatattata aaatcactga aatagggcgt aatcttttag aagaaaaaaa gcaagagtgg
    11281 ctgacgacga aagacgtagt cgatcaattt ttggagggaa attaaatgga tacaatccgt
    11341 aattatttag acagtctttt tatcggagta cctcaaagta ccgagataga taagctgaaa
    11401 actgatttgt tggcaaatat ggaagaccat tatcatgaat tgatgggaga aggaaaaaat
    11461 gaacaggagg ccatcggtac agtaatttcg acatttggtt caatcgatga attattagaa
    11521 gaactagatg tcgaaaaaaa acatcaagct gacgagacgg aaacaaacac agcttctatt
    11581 tatttaagcg aagcagaaaa ctactggaaa gaatacagag cggcaagtct ccaagtggca
    11641 tcaggtgttc tattcattag cttatctttt gcaagttttc ttttcttttg ttcagcagga
    11701 tatgtgttca tgggtatttc ttgtctgatt ttcggtatag cattagctgt cggttttttt
    11761 attgcttctg gtatgaaaat cacacgttta aatcactttt tgcaccatcg gaaaatacct
    11821 gaaaaagtat tagcagaagc aaaagaaaaa gaagaagagt atcagcgaag ttttggcttc
    11881 tcactgattg caggaattgg attatgtatt ttttcacttt tcccactttt ggcttcttta
    11941 atgtggtata tggatggtag tatcggtgcc tctatatttt ttgttacggt aggaacgggt
    12001 gtcttcttga tcatttatgg cagtctagtc cgtcattctt atcgtcaatt cactcaaagc
    12061 gcttattact ggtgaatgta ttcagatttg taaaaatatt ctatttatcc ggcgtagaaa
    12121 aaaagactgt gacacgcttt tgtcacagtc ttttttatat ataataattt aaaaattcat
    12181 catctaaatc cgtagaattt tctagattgc ttacccatgt aaaatcgtta tagacagatt
    12241 tcagttgact tctgcgccat tgtacaggga taaaaagacg catgctatct tcattgaatc
    12301 cgactaataa tttctttccg tcagtaatga tgggacgttg taacaaagta aaatgcgaga
    12361 cgatcagatc gacaagctct gaggtcgtac attggtcgat ttggtctttc aaatttttat
    12421 aatcaatcga gcgtttagct aatagatcgt caaatccgtt ttctgttaat gacaataaat
    12481 aaaatatctc tttccttgtt aaaggctgtt taggtgtgaa aattcgttct ctgtaaggca
    12541 tttgatggtc tttcagccag tttgccatca gatggtaact atgtgtgcga gaagaataga
    12601 tcgtaagcat aaaaaaactc cttaaatagt ccattaattt gttagatctg ttgagataaa
    12661 taataggtaa ggagctgtgc ataagcattg atcaaaacaa cccaaacatc attagaaata
    12721 actttctaaa gtcttaaact tgcgacgaga agcaactaga ctaatagaaa aaatgtctta
    12781 gccatttggc ttcctgtttg tttttttgct tatgctgcag ctcctcggga aagggttcta
    12841 acaatttgaa gaaggttgat gattgatcta gtttaaattg tgtgaaattt gaaatctggt
    12901 gtttttacta gatgaatcac ctaacaacta tgattataac ttatagaact tctgttttgc
    12961 attcggtttt gttatagttg ttataaagtt ttattttatt acagaacatt tt
//
LOCUS       DASJTM010000077        12730 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJTM010000077 DASJTM010000000
VERSION     DASJTM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.077"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..177)
                     /locus_tag="U4B29_002139"
     CDS             complement(<1..177)
                     /locus_tag="U4B29_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2107076.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="U4B29_002140"
     CDS             319..933
                     /locus_tag="U4B29_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2107077.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="U4B29_002141"
     CDS             1122..3002
                     /locus_tag="U4B29_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2107078.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="U4B29_002142"
     CDS             3081..3308
                     /locus_tag="U4B29_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2107079.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="U4B29_002143"
     CDS             3313..3855
                     /locus_tag="U4B29_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2107080.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="U4B29_002144"
     CDS             4082..4798
                     /locus_tag="U4B29_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2107081.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6876)
                     /locus_tag="U4B29_002145"
     CDS             complement(4858..6876)
                     /locus_tag="U4B29_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN2107082.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="U4B29_002146"
     CDS             complement(6889..7887)
                     /locus_tag="U4B29_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107083.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8085..9326
                     /gene="sstT"
                     /locus_tag="U4B29_002147"
     CDS             8085..9326
                     /gene="sstT"
                     /locus_tag="U4B29_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN2107084.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="U4B29_002148"
     CDS             9473..10108
                     /locus_tag="U4B29_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN2107085.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="U4B29_002149"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="U4B29_002149"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2107086.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..12563)
                     /locus_tag="U4B29_002150"
     CDS             complement(11184..12563)
                     /locus_tag="U4B29_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107087.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT         4253 a         2025 c         2495 g         3957 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc
    12481 atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa
    12541 cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat
    12601 ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa
    12661 ctccatacat tatctagtcc tatgattcga gataaatgaa agtaagtatc ctgattgaca
    12721 ctatgataga
//
LOCUS       DASJTM010000078        12600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJTM010000078 DASJTM010000000
VERSION     DASJTM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.078"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="U4B29_002151"
     CDS             complement(324..893)
                     /locus_tag="U4B29_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2107088.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="U4B29_002152"
     CDS             complement(967..2256)
                     /locus_tag="U4B29_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107089.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="U4B29_002153"
     CDS             2610..3458
                     /locus_tag="U4B29_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN2107090.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="U4B29_002154"
     CDS             3472..4545
                     /locus_tag="U4B29_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN2107091.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="U4B29_002155"
     CDS             4535..6706
                     /locus_tag="U4B29_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEN2107092.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="U4B29_002156"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="U4B29_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN2107093.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="U4B29_002157"
     CDS             complement(9117..11303)
                     /locus_tag="U4B29_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN2107094.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="U4B29_002158"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="U4B29_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN2107095.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="U4B29_002159"
     CDS             complement(11525..11965)
                     /locus_tag="U4B29_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN2107096.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="U4B29_002160"
     CDS             complement(12039..12512)
                     /locus_tag="U4B29_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN2107097.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3537 a         2652 c         2235 g         4176 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
//
LOCUS       DASJTM010000079        12470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJTM010000079 DASJTM010000000
VERSION     DASJTM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.079"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..760)
                     /locus_tag="U4B29_002161"
     CDS             complement(107..760)
                     /locus_tag="U4B29_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2107098.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1048..1568
                     /locus_tag="U4B29_002162"
                     /pseudo
     CDS             1048..1568
                     /locus_tag="U4B29_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1470..2186)
                     /locus_tag="U4B29_002163"
     CDS             complement(1470..2186)
                     /locus_tag="U4B29_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN2107099.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2236..2445)
                     /locus_tag="U4B29_002164"
     CDS             complement(2236..2445)
                     /locus_tag="U4B29_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107100.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            2722..3995
                     /locus_tag="U4B29_002165"
                     /pseudo
     CDS             2722..3995
                     /locus_tag="U4B29_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4102..5133)
                     /locus_tag="U4B29_002166"
     CDS             complement(4102..5133)
                     /locus_tag="U4B29_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107101.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5140..5970)
                     /locus_tag="U4B29_002167"
     CDS             complement(5140..5970)
                     /locus_tag="U4B29_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107102.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5967..6782)
                     /locus_tag="U4B29_002168"
     CDS             complement(5967..6782)
                     /locus_tag="U4B29_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2107103.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6779..7603)
                     /locus_tag="U4B29_002169"
     CDS             complement(6779..7603)
                     /locus_tag="U4B29_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN2107104.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7593..8693)
                     /locus_tag="U4B29_002170"
     CDS             complement(7593..8693)
                     /locus_tag="U4B29_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2107105.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8871..9656
                     /locus_tag="U4B29_002171"
     CDS             8871..9656
                     /locus_tag="U4B29_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN2107106.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9689..10072
                     /locus_tag="U4B29_002172"
     CDS             9689..10072
                     /locus_tag="U4B29_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2107107.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10148..10279
                     /locus_tag="U4B29_002173"
     CDS             10148..10279
                     /locus_tag="U4B29_002173"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2107108.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10248..10484
                     /locus_tag="U4B29_002174"
     CDS             10248..10484
                     /locus_tag="U4B29_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2107109.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10562..11533)
                     /locus_tag="U4B29_002175"
     CDS             complement(10562..11533)
                     /locus_tag="U4B29_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN2107110.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12185..>12470
                     /locus_tag="U4B29_002176"
     CDS             12185..>12470
                     /locus_tag="U4B29_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107111.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3743 a         2809 c         2289 g         3629 t
ORIGIN      
        1 tatgttttag gaaagataaa aaaaattaat gaatgaacta atataattat tattccaaga
       61 acagaaaata tttgtatgaa catttttgga aatatcttta attttttcat tttctctcca
      121 atttatatcc tacatttctt atagtagtta tacagtctaa ttgtaatttt tttctaagtt
      181 cttttatata tacatctatt actcgatcat aaggagtttc ttcgctatct ttccatacat
      241 aatcaataat ttgcattctt gttaacactt gtccattatt atccaataaa cattttaata
      301 cttctagttc ttttgcattt acatctattt cttcaccatt tattttagct gtatagctat
      361 taaaattaac cgatagatcc ttatattcaa acttctctaa atgtccataa ttcttcttaa
      421 ttaaagaatc tattctagct tttaagaccg gcaatgaaaa tggcttttct acatatccgt
      481 ctactaaatt agtaaatgca tcaattttat attcttcatc actaaatgca gtcaagatta
      541 gaattggtaa attgcttttc tttctaattt ctttcaacac ttctaaacca tttataaaag
      601 gtatctgaat gtctaagata accaaattta tatcgctatt aaattttgac aaggcttctc
      661 ttccatcttt agcttgaata acagtatatc caaattctga aagatattca cttattccct
      721 ctcttatcat attatcatct tcaacagtta atattttcat atatacctcc acttaagcct
      781 atacactaga tattatacag catattatgg ctctataata tctagtgttg gtgacgcctt
      841 ctgttgccaa tactttttct tttttttaat aaatgcaaac agacaccttg aaatccttta
      901 gaaaaaaggc ttctataata ttttgtgttg ttggaaaatc gaaggcgatt ttctactaac
      961 actttttctt cgtttcaact caaaaagaga ctcacttttg gtaaaattta gtcgaccaaa
     1021 acatcaccct accaaaggag tcgctctatg gataatcata ctagaaaatt actcggatta
     1081 acagataaac atttattttt tgatgaagaa tggctgattg aaaaagaata taaaggcgtt
     1141 caagcgcagt ttattaaagg gagattggat gactcacctt cccattgtga acactgtgga
     1201 tcgattcgta ttatccgcaa cggttcctat acgacacgta ctcagatgtt gaaagtcaaa
     1261 gaaaagttga ctattttaga attaaaacgt acccgttttc tatgctatga ttgtgggcag
     1321 accttttctg cgaaaacgga cttagttgat gaacatcatc aactaacaaa ggaactgaag
     1381 caggcgattc tcatagaact gtacgaaaat caatcacgta atctgaaaat cagaagaagt
     1441 accgtcggct caagcgatat tggaaactct tactcaaaga ttccacgaca ttggagccgc
     1501 tcaaacggca ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgtcgatg
     1561 aaccgttgtg cacaatggtg tcctgaacgt acagcgacac cttccatatc cagagcagtc
     1621 gccacatcac ggggatggat gccatccaaa ttaaaagcaa ccacgccagt atgatgttca
     1681 ggatcttgcg gtccgtaaat ggtgataccg ccgatcgctt gtaatttagg taaaacgtag
     1741 gcaaccagtt cttgctcata acgatgaata ttctccatac caatctcatt caagtaatcg
     1801 attgctcctg caatgttcgg agtaccagct tcaaatttcc atggcagctc tttccatgaa
     1861 ctatcgtaaa gattgacaaa atctatcatt tcaccgccga attcgactgg ctccatttgt
     1921 tctaacaatt gacgtttgcc atataataca ccgattcctg tcggcccgca cattttatgc
     1981 ccgctaaagg cgaaaaaatc tgcatccaac gcttgtacat caactgtcat atgcggtgct
     2041 gcttgggcac cgactggact atcctcaact acaacagcct gttcaaccct cagccgtttc
     2101 aagcacaaca aataaccttc tggatctggt ttgccgacct gaagatcatc gatcgtcaca
     2161 aaggaatcaa ttttttcctt aatcatcgga tgtgcttcaa aaaaatagtc tagctcccgc
     2221 agttcagaat tgagactact ccgattttcc agtgctgctg atccgctgct caaaatgttc
     2281 agcatccact ggtctcccaa ccgcttgact tatcccttca taaaaggaca gttctgctgc
     2341 cgtcccaaca ccttgttgat agtctgcaaa aggaatttta tagccgaact gctccagtaa
     2401 ataggcttca gtaaatagtg atgaatactc tcgtaaaaca tccattcact gtcaatcatc
     2461 gtcccatcaa aatcaaaaat aagtgctttg gtcatcttct ttccccctta cccaaaacaa
     2521 gcaaaagctg tgtcctgctc tctctacagt atcagcataa aaactgccgt ctgaaaagtc
     2581 aaaggtgaaa gaaatgtggc gctataattt ctaggactga tagtaaatca ttctatcagt
     2641 ccttttgagc ataaaaaaac aaaacataca gaagctccgt tagaattgaa tcaccacaac
     2701 acaatcaaag gagcgttctg tatgcctgat cttcatctta tcaaactatt actaggaatc
     2761 aaagatggaa acattcaaat cactcatgtc gagaagaaaa aaatacattc aatcaacagt
     2821 atcgttgttt ctgccacgct cattaaaacg attcagccct gtcctcagtg taaacaggtg
     2881 aatcaacatg ggctgatcgt taagaacgga aagaaaaaat cgctggtcct tctcaataag
     2941 tgtgcgaatc aacggactta tcttgctttg gcaaagcaac ggtacctctg tcgtggctgt
     3001 cacacctatt ttacggcgaa tacctacatt gttgaaaaaa actgttttat cgccaaacaa
     3061 gtacattata aaatattgga ggaattgact gaaaaacaag caatgacgac gatttctaaa
     3121 cattgcggcg tttcttggtc aaccgtttcg cgaacactcg cttctctgct ccccatgaca
     3181 aacgtgaaaa taaattggtt gcctcgctgt tcattggtag atgaatttcg ttcattgaaa
     3241 aatcaagtcg gtccctattc tttttcttgt atggatggcg atactgggaa attactggat
     3301 attcttcctt cacgtaagaa gaaagatctc atttcttatt ttatgcaatt tgaacgacgt
     3361 gcacgtttaa acgtaaaaat actcgtaaca gatatgaacg cctcttatgc tttgctgata
     3421 aaagagtgct ttcctcatgc caaactcgtt gtggatcgct ttcacatcgt caagcacctc
     3481 attcgcagtt ttaaggatat ttgactatga gtcatgaaaa gcttcgaccg aaacgatcca
     3541 atacaagcca aacactatcg acaagtgaag gctttatctc gacttctgat caaacgacag
     3601 gacacgcttg tttatgataa atggacaaaa tggcgtaatt ttggttgggc ctatttaacc
     3661 gaaagtgaag tcgtagaacg tctactttct acttctgatg aactacgaat agcttatgct
     3721 tactatcaag aaattcttca agctttttat gacaaagaag cggacatctt ctttcagctt
     3781 gtaaaaacaa tgcccaagtc ggttccaaga gaactgcatc atataaaaaa agcgtttata
     3841 aactatgaat ccggcattcg tttagcctta gagttgcctt actcaaatgg caaaatagaa
     3901 aatctgcata cccacatcaa agcattgaaa cgaatagctt acgggttagg atgagaacca
     3961 gaattttctt aatcaatcat ctgatcactt attaaacata aaaaaacatc taaagcagaa
     4021 aatcaattct accttagatg ttcttatatc actctatcag tcctatttga caaagagcca
     4081 gaaatgtttg ccgactatac actagctttg ttgtgcaaaa tgctgagtat caatttccac
     4141 agggattgct agaatatctt tcagctgaat gtataaggca tcgctaattt tctcttcccg
     4201 atcgttcaac cgatccacta aaatttcttt gccctccagc gtttgaaaaa cataccgttg
     4261 aatgttgcct aaaataaatg attccttcca tagaaccgga atcttaaccc cctgaggaac
     4321 cggttcaaat tgaatcagct ccggtcttaa ataataaatg cagcgattgc ccttagcgga
     4381 aaactcgtgg gtatactgat tcagctcata ccattcgaaa cgattatagt tgccgataaa
     4441 ttccgcaaca aatcggctct gaggattctg atacaattcc ttcgaggtgg aaacctgagc
     4501 cacccgccca gcttccatca caaaaatccg atcactgatt cgcatcgcct cttcttggtc
     4561 atgagtcaca aaaatcatcg tcatccctag cgattgctgg atagcgcgca aatcccgctg
     4621 cagctgtttt ctaattcggg catccaaagc acttaatggc tcatccagca gcaggacttt
     4681 cggttcagtg accaatgagc gagccagtgc cacccgttgt cgttgaccgc cagaaagaaa
     4741 ctggacattt ttatctgcct gctccgtcat atccaccaat gatagcatct caaaaacctt
     4801 ttcctgaatc acctcaggac tcagcttctt taccttcaaa ccaaaggcta cattttcaaa
     4861 aacagtcata gttggaaaca atgcatactg ttgaaaaaca aagccgatat tgcgatcctt
     4921 cgtaggctta tcggcaatat tctcattgtc cagcacaatc tcaccgctct ttggttgttc
     4981 caatcccgtg atacagcgca gcaacgtaga ctttccgcac ccgctgggac ctagtaaagt
     5041 aaccagctca cccttttcaa tctgaatttc atcaatagct aaaatctgtt tttcatcaaa
     5101 aaacagattg agatcatgta tttctaaaaa cattttccgt cacaactcct ttaagggctt
     5161 ctttttcgac tgcttctctt ccctcgtctg cttaagggac agctgttgct tttgtcgata
     5221 agtcagcata aaggtagtta aagcaattcc caccataaac agcaaataca aaaccatcac
     5281 agcgctagcc aaatggccgt tttcgttcat ccgccgcagc atatagatgc gtaccgtctg
     5341 aaatcgaccg ccaatcaaca ggttcgtcag catgtactcc ccaaaaaccg aagaaaaaat
     5401 catcagagaa actagggtca caccgatacg gatgtttgga aacagcacct ggataataat
     5461 cgtagacatc ggggcgccca aggtttctgc tgcatccact aattccttca gattaattaa
     5521 gcgcatctgg ttgttaagac taagatagac aatcggcaac gccgtaataa acaatgcacc
     5581 taccaacact acaaacattg ggataccagt ttcagcatac gttcgaagca aagccgttac
     5641 caaaatcact cctggtaaag catacggcag caggagcagc ttttccagcc aacgattcaa
     5701 acgagggaaa tacaaatgaa tccaaataat ggtgggaatc atcaacaaca gaatcgtcac
     5761 caagaccaca cctgctaata aaagtgagcg acccaccgca gcaataaatt ccctgtccgt
     5821 cagcagctgt tggaaccatt gcaaggtaaa atcactaggt aaaagcgatt ttgaccattt
     5881 agtggagctg gcatacaaaa tcgttccaat aatcggcagc aagtaataca ggacaaagca
     5941 gaacaaaatc ggttttatta actttttcac gtctgcacca ccttgccttt cttttgaata
     6001 atcaacattg ccgttaccag caggacacct aaagcgactg ccaacgcact gcctaaatta
     6061 ggcttggcga acacatctcc cgacactaaa gccgcaattc gaatcgttaa aagatttaca
     6121 ttactgctgg tcagggcata cgctgtttca taggtcccca tgccattagc aaataagatg
     6181 acaaaggtgc tggcaatcga tggtcgcaaa aaaggcaaag ccaccttgaa ccaagcatac
     6241 gctgatgaag cgcccagcaa ttgtgcggac tccagccatt cctttttcac ttccttcatg
     6301 ctgggataca aaaacaagac ccctaacgga atttggaaat aggtatacgt gatggtcaat
     6361 ccccaccaag aatacaaatt gaagtccgct agaaatggca aaatcatttt cagcacacct
     6421 acattcccca acaaaataat aaaagagaat gccagcggaa tcccagcaaa attagctgcc
     6481 aaattggaaa caaaggtgat tttctcctgc aatttagtcg atagattcat taagcataaa
     6541 cataaaataa acgtcccgat taacccaatc acactggagc tgacagcaat cagtacgctg
     6601 ttaacaaagg cctgtgtgta atatttgttg ctgaaaatat cactgtaatt ggccaatgtc
     6661 cactggttag ttaaatcact gtgcaagctg cacagaacca taaaaagcag cggcagcagt
     6721 aaaaatgcac agaagataaa ggaggcagga accagaacca agcgtttttt catctctttc
     6781 atcccctcat caattcttga atgtcctgca ttttctctga cggtgcaatt cctaataacc
     6841 agcaaaacaa tggagccgcc agcagctgtg gtacagtttg ttccttcagc ttttggcgtt
     6901 tatttgaaat caaataaaaa ggaacttcac gatgtgcagc caagctgccg ccatgcagtc
     6961 cgtgcgcatc catcccgtga tccgcagtca ccacgacttg ataacctttt tgcagccatt
     7021 caggaaggta aatagccaat aatctgtccg cctgattaac cgctgctgca tattcatgag
     7081 aatcggcggt aaatttgtga ccgacatcat caatattcat cgaatgtacg accaaaaagt
     7141 ctggctgata ctgattcact aaataatggg catccgcaaa taaatgcgaa tcagggtaag
     7201 tgtcctcact ataaaagatg ccatgctgaa tcagtcgctc ctcctgatgc tggatgcgat
     7261 caaccaaacg attatagggg gcttgattgt acaactcgct gtaccagtgg taagcggcgg
     7321 ctcctgtttt tccgccagct tccttaacta attcaaacag cgaccactct ttggatcgct
     7381 gagcatggta attactatag atgccatttt cataggctgg aacgccggtc atcagtactt
     7441 cgtataacgg acgtgaattg ctgggcagct cactttttac cttcaataag cttgcttgct
     7501 ggtgctcaac taaatgattc atgaacccaa gctgggcgac ggctgtgtcg tagcgacagc
     7561 cgtctagtat aactacaatc agtttgtcaa gtctactttg cataggtcag tacctccgcc
     7621 tgccaaagct ctggaagctc tacagaggtc ttgttccaag cttcattgtc ttcaactggc
     7681 accgccgctt cataagcatc ctccggcagt aatttttcag ctacttcttt aggcagctct
     7741 accttgcgaa taggtcgcgc atagcccttt gccaaattga tttgcccttc atcagataaa
     7801 atatattccc gtgctagctt tgccgcattg ggatgagggg catttttatt gattaaggtt
     7861 gtatacccgc taatgatgga gccgtctgtt ggcacagtta cctcaaaacg ctcggagtca
     7921 atttggtcac gataatttag tccattgaag tcccaaacaa ctgccacatc gatttcacct
     7981 ttttcaagat ttgctacgct ggcatcaacg gtggacaaac ggcctttttc tgccattttt
     8041 ttgtagtaat tcagacctgg ctgaatatcc ttttcgtcac ctccgttggc aatcgccgca
     8101 gccaatacag caaattgtgc ttggttggca atcgtcacat cccccacgct aacttgatag
     8161 tcaccctctt gcagctcctt ccagctagtc ggtgcttgct tcacattctc tttatcggtg
     8221 ataaaggaaa tggttcccgt atagcttaac aaccaatccc catcatcatc ctttgcccaa
     8281 tccggaattt cctcccaata gttggtctta taaggtaagg ttaactcttt gctttcagcc
     8341 aacggaccga agctgatacc gacatcacca atatcggcgg ttccattttt tccttctgat
     8401 tcaaacttag ccaattcttc tgcactggac atatccgtat ccacgtgact taagccgtat
     8461 tctttttcaa ggtcgttcca agtgcctacc caatttgccc aagtatccgg cattcccaag
     8521 ctggcaatct ctccttcttc cttggctttt tctataatcg tatctaaaga gtcgtctgcc
     8581 gcagccgatt gcttcgttgc ttcacctgag ccgcagcctg tcagcaggat acttactgtt
     8641 gctgcacaaa ataaaccgat agtcatgtta cgaatttttt cattcatttt cattcattct
     8701 tctccccttt cgataattcc attctaacaa agctttgtaa tgagagaatc agtttcttga
     8761 aaaggtttca tgaacaatgt aaaggttgta tatggtttgt aaatttcgac gaattcccgt
     8821 taaccttcta aagcttttgc ggtatgatag tagaaaaaag gaggctccct atgcagtatt
     8881 atgatcagca tctccacacg tatttttcct ttgattcaga ggaaaaattt gaaaactatt
     8941 tagcctatca gccggagttt ttcgtttcta cggatcactt tgatttaaac aatccatcta
     9001 ccggaaaaga tgacattccg gattatccag cctatataaa caaactggac tctttaaaag
     9061 aaacctattc caccaaattt ctgcgaggta tcgagattgg cgttgttcca ggacaagaag
     9121 agcggatcgt ggattacctc gctcagcatc cttatgattt aaagctggta agtattcatc
     9181 aaaatggccg ctttgattat atggacgatg tggttttaac caaagatccc attcaaacta
     9241 ccaaggaata ttttgatcag atgttgcagg tcttgacgga ttttccagaa ggagatattt
     9301 tgactcactt tgattatgga ttacgccgct tcaccttcac agcggaagaa ttcgcggagc
     9361 attttgaggc tcagctgcta acgattttca aaaaggttgt tgaacgagaa ttggcgatgg
     9421 agttaaatgc caagagcttc ttgaaatacg gcaacgaaga cctttaccgt tatgccgttc
     9481 ctttgtatca aagtgtcggc ggaaagctct ttaccttagg ctcggatgct catgttgccg
     9541 aagattatca gctgggcttt aaaaaaatga gtcagctgtt ggcagactgt caggtagaaa
     9601 agttgctagt cattcaagga aaagaacgcc gcctgacgcc gctgccgatt ttgtgattcc
     9661 tatataatag aagaaagtgg tgaaaaaaat gagcaacctg tgggactaca atcaagaggc
     9721 acccatccat tatttaattg cgcgacattg ggatgcgttg aaaattgagg cggtttgccg
     9781 atccttgtta gcagccgttc caaaacaaca actggaaaat tttttggtgg ctgacagctt
     9841 gcagcgggaa aaagttcagg cttattttgc agcctttaaa gatcagcctt tggaatattt
     9901 gcatgcgcag tttcatcttt tttatcaagt agccgcccca gatgactaca acgatttacg
     9961 aggacagctg caattgacct ttcaagcgga tgaaaccgcc tataccgttt tattgggaat
    10021 ggctcgcctt ggtgatcagg cgaaggtcga gtggcggatt ttcgatattt gatggcagga
    10081 tcaaggcgtt tcagttgcaa accttcctct ctttcaatta gaaaaaccgc agcaggaaga
    10141 agtttgtgtg ctggtcaccg atgctggtga aattcgtctc cgcttatttc ctgaccaagc
    10201 tcccctggct gtagccaact ggcgctccct agctgagcaa ggcgtttatg acaacacacc
    10261 tttcggctcg tgtcattaag gaatttgtta ttcaaggtgg cgctttggat ggttctggtg
    10321 atgaagctgt ttccagctat gagggctttt tcgctgatga agtgcatcgc ggactttatc
    10381 attttaatgg cgccttggcc ttaggggacc acggccccca caccaacggc aaccagtttt
    10441 ttattgtgca aaacacaaag gcgcaggctg acctgctaat gtaaagcaag catacttgga
    10501 gcttggagga ctaccggagc tggatggtcg ttatactgtt tttggacagg tataccaagg
    10561 gttagaaatc gtagcagcga tcgcagctca gccgacaaac agtgaagatg taccgattga
    10621 gccagtgttc attcgacaaa ttaacttcga aaaaacatcg taaaaagaca ctcactgact
    10681 tcagcaaaag ctctattctt gagctggctg cagctagtga gtgtctttca tttatccata
    10741 agataaagct tggattcaat aaataaataa atgttttggt taaagctgtg accagcaaat
    10801 gaggaaccgt gcgagccagt tgatctgcca atgcaaacat ttctttaggt gaaggcgctg
    10861 gcgtgtctgc caacggatac tccagtccca atgcttggta accgtgcata ccatagcagt
    10921 ggtacggtag taattctaac ggtacgaacc gatcaagatc tgccacaaaa caagcaatcg
    10981 tcagcagctc ggtcaggttc atattgatac ctggaaccac aggaactcga attcgcactt
    11041 cgcagttagg atactcaatt aaaaagcgac gaatattctt caaaatttgt tggttatcta
    11101 agccggtcca ttgtttgtgg agcttgctgg taaacatttt tacatccaca aacaatgtat
    11161 ccacataggg agctaccttt tggatttcat tataagcacc acaaaagctg gtttccagta
    11221 cggtattgat tccaatgtat ttcgattttt gcaaaatttc tttagcaaaa tccgcctgaa
    11281 ccagtgcctc gcctccgcta atggtcactc cgccatccga gtgaaagtaa aaaacagcat
    11341 ccttctcaat ctcatccatt acctcttcag cggtcatggt ctggccaaat cctttttcaa
    11401 tctgcttgct ttgtgattcg ggaatcgaac accaggcaca gcgtaatggg cagcccttta
    11461 aataaacaac ggtccgtaaa ccagcaccat catgtagaga accctgttct attcttaata
    11521 ctgttccttc catatagatt tttgaattac acactagttt aaactaaaac acacaaggaa
    11581 aaacttacca tttaatcata aaattagctc atataaatat actaggatgt aaaatagact
    11641 ggtcaaaatc tggtcaaaaa agaggactaa tacaggtagt cctctttttt gttattttaa
    11701 tattctaaat cattttttat tggtttattc ccttattcga tgtagactca aatcactata
    11761 tcactagata tcaaacggcg tcatacggaa tcaaaaacat caaatttgac ctgttaaatg
    11821 gtcattgaat aacattcaaa atcaataaaa cggagtcaga cacggagtca aaaacgctga
    11881 gagtaaaaaa tatatacagc cttgtaggta aagtaccaca gggctgtata ttttttattt
    11941 cagattacct taaatgattc catttacttt ttatctcatt ttttcctact agttagtatc
    12001 aaatactctc ctattttctc aataaactat ttttggatac atggtatctg aaacagctaa
    12061 aaccttgtca ttacagcaaa aaatcacgtt atccagactg tagacaaagt ccattttatg
    12121 gatgatgtct acagtctttt cttttataat agatttatga aaacgaatgc aaggagtgat
    12181 agctatgatg tctaaaaaca atacgaatgg tagaaatcaa tttgcgatgc ttacgattga
    12241 tgatctagtt cctcaagatc atctcgttcg aaagattgat gccgcgcttg attttgaatt
    12301 tatttatcca atcgtcgaag ccacttactc cgatttaggt cgcccaagta tcgatccagt
    12361 gattctgatc aaattggtgt ttattcagta tttattcggt atccgttcca tgcgacaaac
    12421 cattaaggaa gtcgatacca atgtcgctta ccgctggttt ttaggctact
//
LOCUS       DASJTM010000080        12344 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJTM010000080 DASJTM010000000
VERSION     DASJTM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.080"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(356..970)
                     /locus_tag="U4B29_002177"
     CDS             complement(356..970)
                     /locus_tag="U4B29_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2107112.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1576..1953)
                     /locus_tag="U4B29_002178"
     CDS             complement(1576..1953)
                     /locus_tag="U4B29_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107113.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2253..3134)
                     /gene="galU"
                     /locus_tag="U4B29_002179"
     CDS             complement(2253..3134)
                     /gene="galU"
                     /locus_tag="U4B29_002179"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2107114.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3177..4196)
                     /locus_tag="U4B29_002180"
     CDS             complement(3177..4196)
                     /locus_tag="U4B29_002180"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2107115.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4829..5833)
                     /locus_tag="U4B29_002181"
     CDS             complement(4829..5833)
                     /locus_tag="U4B29_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2107116.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5846..6895)
                     /locus_tag="U4B29_002182"
     CDS             complement(5846..6895)
                     /locus_tag="U4B29_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2107117.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6912..7733)
                     /locus_tag="U4B29_002183"
     CDS             complement(6912..7733)
                     /locus_tag="U4B29_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2107118.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7720..8499)
                     /locus_tag="U4B29_002184"
     CDS             complement(7720..8499)
                     /locus_tag="U4B29_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107119.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8516..8986)
                     /locus_tag="U4B29_002185"
     CDS             complement(8516..8986)
                     /locus_tag="U4B29_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107120.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8998..9417)
                     /locus_tag="U4B29_002186"
     CDS             complement(8998..9417)
                     /locus_tag="U4B29_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN2107121.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9498..12275)
                     /locus_tag="U4B29_002187"
     CDS             complement(9498..12275)
                     /locus_tag="U4B29_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2107122.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3605 a         2599 c         2026 g         4114 t
ORIGIN      
        1 acaaatgtac tataattact ttgtaaagag ttttggggaa gactctttca tcaacgtgta
       61 ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca ctacttttta
      121 tttttctgac actacatatt cttataactc atttctcata actgtgaaac aacttataaa
      181 aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt aaaataacta
      241 aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt tttcaaacgc
      301 tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg gataactatt
      361 ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc tgcagcaaaa
      421 agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc aaagtcaaga
      481 ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca aataatcgca
      541 aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta acaatgatta
      601 atggatgacc attttctttg atccactgag ctagttcgat gattgccggg tttcttcctt
      661 cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca ataattgcct
      721 gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc aatatacttg
      781 gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt gtgcggctag
      841 catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg ccagcttgaa
      901 gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag tcaaaataac
      961 ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac agctgcctaa
     1021 tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc tttgctgttt
     1081 caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca aataaaacaa
     1141 cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga ttaggtacaa
     1201 taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg tgtatgtgtg
     1261 tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt tttttattca
     1321 gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc aagaaaatac
     1381 aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga atggcttatc
     1441 ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa aagctttttt
     1501 ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc tttttttgcg
     1561 tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga gagaatctgt
     1621 actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc ttttcatata
     1681 tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc aagcttccca
     1741 actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg cataccagat
     1801 aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata gactaataag
     1861 caatagtggc aacacaaata tccaattgct tccttccatc agccacttga gtatatcaag
     1921 cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt ttttataatt
     1981 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
     2041 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
     2101 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
     2161 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
     2221 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
     2281 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
     2341 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
     2401 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
     2461 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
     2521 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
     2581 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
     2641 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
     2701 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
     2761 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
     2821 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
     2881 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
     2941 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     3001 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     3061 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     3121 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     3181 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     3241 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     3301 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     3361 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     3421 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     3481 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     3541 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     3601 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     3661 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     3721 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     3781 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     3841 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     3901 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     3961 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     4021 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     4081 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     4141 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     4201 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     4261 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     4321 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     4381 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     4441 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     4501 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     4561 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     4621 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     4681 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     4741 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     4801 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     4861 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     4921 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     4981 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     5041 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     5101 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     5161 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     5221 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     5281 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     5341 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     5401 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     5461 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     5521 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     5581 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     5641 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     5701 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     5761 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     5821 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     5881 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     5941 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     6001 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     6061 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     6121 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     6181 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     6241 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     6301 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     6361 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     6421 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     6481 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     6541 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     6601 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     6661 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     6721 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     6781 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     6841 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     6901 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     6961 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     7021 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     7081 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     7141 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     7201 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     7261 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     7321 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     7381 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     7441 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     7501 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     7561 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     7621 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     7681 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     7741 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     7801 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     7861 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     7921 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     7981 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     8041 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     8101 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     8161 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     8221 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     8281 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     8341 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     8401 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     8461 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     8521 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     8581 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     8641 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     8701 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     8761 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     8821 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     8881 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     8941 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     9001 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     9061 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     9121 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     9181 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     9241 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     9301 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     9361 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     9421 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     9481 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     9541 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     9601 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     9661 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     9721 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     9781 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     9841 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     9901 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     9961 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
    10021 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
    10081 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
    10141 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
    10201 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
    10261 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
    10321 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
    10381 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
    10441 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
    10501 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
    10561 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
    10621 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
    10681 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
    10741 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
    10801 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
    10861 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
    10921 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
    10981 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
    11041 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
    11101 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
    11161 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
    11221 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
    11281 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
    11341 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
    11401 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
    11461 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
    11521 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
    11581 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
    11641 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
    11701 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
    11761 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
    11821 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
    11881 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
    11941 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    12001 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    12061 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    12121 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    12181 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    12241 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    12301 tcattttgtc aataaaatct ttaataaaga attgaaaaat atat
//
LOCUS       DASJTM010000081        12211 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJTM010000081 DASJTM010000000
VERSION     DASJTM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.081"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            452..1267
                     /gene="thiD"
                     /locus_tag="U4B29_002188"
     CDS             452..1267
                     /gene="thiD"
                     /locus_tag="U4B29_002188"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN2107123.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1596..3047)
                     /gene="cls"
                     /locus_tag="U4B29_002189"
     CDS             complement(1596..3047)
                     /gene="cls"
                     /locus_tag="U4B29_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2107124.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3388..6054
                     /locus_tag="U4B29_002190"
     CDS             3388..6054
                     /locus_tag="U4B29_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2107125.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6418..7170
                     /locus_tag="U4B29_002191"
     CDS             6418..7170
                     /locus_tag="U4B29_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2107126.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7192..8346)
                     /locus_tag="U4B29_002192"
     CDS             complement(7192..8346)
                     /locus_tag="U4B29_002192"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN2107127.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8531..10972
                     /locus_tag="U4B29_002193"
     CDS             8531..10972
                     /locus_tag="U4B29_002193"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN2107128.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11129..>12211)
                     /locus_tag="U4B29_002194"
     CDS             complement(11129..>12211)
                     /locus_tag="U4B29_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HEN2107129.1"
                     /translation="TQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTK
                     ELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFK
                     SMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNAS
                     YCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYW
                     KLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKR
                     DTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIK
                     RIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3913 a         2182 c         2601 g         3515 t
ORIGIN      
        1 ccttcttcat gtaggaaata gattattcct ttttctgtgt tttgcgtata agcttttata
       61 ctcctgtctt ttttgaaaga acagatcgta tagccatcta tttggtctgg tagtcgtttg
      121 attttcttca aaatgacttt tttatcctca agtttcattt ttttcacctg actttatttt
      181 tttcatatgg aaatcatagc gcctttggtt atatgaaaac aagaaggata ctgaaatata
      241 tataaatcaa atagtggtta tttattatta gaatagcgaa tacaaataaa cgaccactat
      301 tttctgattt attgcaaatg ttttttcaaa taaatttgat taccaagaaa aggtagtggt
      361 atatttactg agggatactt tgaaaaacat atgatagaaa cagtaacagt attatgaaaa
      421 atctttttat aaaaatggag gaatcgattt gatggaagat cagtatgtac aagcaatgac
      481 gatcgcagga tcagatagcg atggaagcgc agggatgcaa gcagatatgc atacattctt
      541 tacaagaaat gtttacggtg tttctgtcat gaccgcatgt gttgcaggta attcttacgg
      601 aatcggagca agtgtgacat tgccaacaga ttttatcgac aaagaatttg aactgattgc
      661 gaaggacttt caaatacgtg cagctaaaac aggtatgcta gcagattcaa agttgattga
      721 gacagttgta aaaaattacc aaaaatatga ttttggacca ttagtcgtgg atcccgtaat
      781 cgtaacgaaa catggaaatc ttttactaga agaatctgct ttgcaaagct tgaaagaaaa
      841 gcttgtccct ttggccgaag tactgacacc taatttttat gaagcagaaa agctgactgg
      901 acggacatta gcagctgaag aagattttgt gcaagcagca aaagaactgc aagatatggg
      961 tgcaaaaaac gttatgatca aaggacatca tgctaaaaat gcgcagcaag aaacagtcaa
     1021 tgactacgta ttgttcgaat caggaaaatc attttggtta aatgcaccat tttatcctac
     1081 cgaccggatc aacggaactg gcgattcgct atctgcatgt attgcagctg agttagcgaa
     1141 aggaaaaaca gttgaacaag cgatccgatt ggcaaaagac tatgtaaacc gtgcaattcg
     1201 tgaagaaata aatgtagggc acaaatatgg ccccatcaat cattgggcag ctggtgaatt
     1261 agagtaaaag aaagaagttg tgaaaaagtt gccctgcatc aagtaataag aacagccaag
     1321 taaaaacagc ttcttatgtt tttaacttgg ttggacttat taccgcagat gcaagctttt
     1381 gaacaccgtt taagaaagag gtcgtgagcc tgccgtccag ctccgagtca caaggaacaa
     1441 gttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca
     1501 ggagaacacc gtttgttcgg aagaagggac tgtgacaaga tttttgtcac agtcccttta
     1561 atcttagtat atttttctct acttgaataa aaaaattata ggattggaga aaataatcga
     1621 gaaatttgtt gcttgaataa tagccagtta gacatttcct tgactgtttc agttgtcatg
     1681 gatgaacaat gtgtctcatc ttctttgaac tgagtagcaa gttcttctaa aaactcagga
     1741 tcgtaaatga cagcacttgt ttcaaaattc aatcggtagc tacgaatatc ttgatttgct
     1801 gaacctacca tgcagatttc atcatccatt atcaatgttt ttgcatggag gaagccgttc
     1861 tcatagacaa ggatttgtac attttccttg atcagctgcc gagcataata ttgtgttgct
     1921 cgataaataa aaggatgatc cggtttgttg ggaatcatga ttttcacatc tacaccagag
     1981 gcagttgcga tttttaaagc agcaatgaca ctttcatcag gaacaaggta aggtgtctgt
     2041 atccaaacac gttttttagc agaagtaatc aatttgataa atgaaagctt gatttgttcc
     2101 ctttcgttat tcggtccgct tgaaacaagc tggatggatg tatcagcaag cttagattca
     2161 tctcgatctg cttttttaaa gaaataattc aaatgatagc cgaccttttt ttcttcaggt
     2221 accgagacgt tccagtctgt taagaagcga agctggagca aagaagaagc ggcaccgaaa
     2281 atccgtatat gcgtatcgcg ccaatagccg aatttttttg ttgtatttac atattgattg
     2341 gcaatattga aaccaccggt ataactaatc tttccatcga tcacaacgat ttttcggtga
     2401 tcatggtaat tcaatcggaa acgaagtaat gccctttgtg aggtaacaaa tgtatggaca
     2461 tgtccaccat ttttgattag acgattgaaa tcttttgctt ttgtgccatg agagccaaat
     2521 gcatcatata atattcgaac ttccacgcct tctgcggctt tttcttctaa aacatgtaag
     2581 acttgctggc cgatattatc tgtcacaaat gcgtaatatt cgatatggat cgaatgtttg
     2641 gcttttttta gatcttgaag cagtgaatcc aatttctctt gtccgtctgt gagaagagtg
     2701 acagaattca ttcttgtcag cggcatacga tttaatgaag agaagaagtc aacaaattgc
     2761 tgtttgtctt tatcgcccat gtcaggatcg taatgttcaa tggtatctcc tctaaaagac
     2821 tgaaagtttt ctaattcttt caagtcactt tgtcggagat agaatttttt cttatccgtt
     2881 aatccacgtc cgaaaaataa atataataca aagcccaccc caggaagggc aaacaatact
     2941 aagagccatg cccaaatggc tgctacatct cggggtttga tcaaaatagc caccaaagca
     3001 ataagtgcat ttaatagata aagggcggtt ataatactag ataccacaaa aatgttcctc
     3061 cttacataca ataacattag tatagcatga tagaagaaag aagcctacca aggtgaatat
     3121 acttttcact cctttcatga aaatgtaggt aaatcgggct aaagtagttg ctttttgtag
     3181 gttaataagg taggatatta gtacagttga atatctcctt aggggagtaa ccggcaacgt
     3241 ttgttgcgat aatatcaaca gtaagttaac caaatggttt tctggtattg tatgaaatgg
     3301 tgagacctaa gacaaatgcg tatttgtctt aggtctcttt tttttcagac aggactaggg
     3361 ccaagatcaa aaaatggggg atgaaatttg gaaaacaagg aacaagcaca acaagcggtc
     3421 tataaagaaa cgatcgactc gttgatgcag caaacgcaga gctcaaccga tggattgagc
     3481 accacgcaag cgaaggaaaa attagaaaga gatggcttga accagctaaa agaaaagccg
     3541 aagaagccaa cttggaaatt gttcttagaa actttcaaag atgcaatggt tatcgtattg
     3601 ctgattgtcg cactgattca gatggtcatg ggtgactaca tcgaattctt agttattttt
     3661 gcagtattga tgttgaattc tataatcagt gttgtccaaa caaaaaaagc agaaggttct
     3721 ttagaagcgt tgaaaaacct ttcagcgccc gatgctcgag tgattcgtga tggtaaggaa
     3781 aaaacgattc cggcaaacga acttgtggta ggagatatcg tgttgttaga cgcaggcgat
     3841 tatgtacctg ctgatggacg tttactagat gcgggatcat taaaagtaga tgaaggtatg
     3901 ctgacaggag agtcgacttc tgcagaaaaa gaagtaacgg atatcacaag tacggtacca
     3961 atcggagatc gtacaaatat ggtattcagt ggaacgatcg taacttacgg acgcggtaaa
     4021 ttcttagtga cttcaactgg gaataatacg gagatcggac aagttgctgg ccttttggaa
     4081 tcaacaactg agcaagtcac tccgttacaa cgaggtctgg ataaattcag taaaaaacta
     4141 agtttggcta ttttggcatt gtctttattg attttaggta tccaactgtt tcgtatttat
     4201 cttggagaag gtacaggaga tatgactgca gatatcgtca gcgcagtgat gtttgccgta
     4261 gccgtagccg tagctgctat tccagaagca ttacagtcta tcgtaacaat cgtgttatct
     4321 ttgggaacga ataaaatggc aaaacgtcat gccattattc ggaagcttcc agcagttgaa
     4381 acacttggtt cgacaagtat catctgtaca gataagactg gtacattgac acaaaacaaa
     4441 atgacagttg ttgactactt tttaggtaat ggcgatactg gggatttcac aaatgaccct
     4501 tcaaaatgga cagctgacga acgtcgcttg atcgaaatca gtgttttagc gaatgatgca
     4561 tcgatcagtg aagacggtac taaattgggc gaccctacgg aagtagcgtt tatcgacttt
     4621 agtgaaaaat taaaccagcc ttatcaggaa atccgaaaca actacccaag acaagcagaa
     4681 cttccttttg actcagatcg aaaattaatg tctactggac acacaattga taacaaaaat
     4741 tacttgtttg ttaaaggcgg accagatatc gtcttccaac gcagtaaaaa agtattgcta
     4801 aatggtgaag tcgttccgat gacggatgaa ttgatgaaaa aattccaaga gcaaaatgaa
     4861 gctttttcaa cacgcgcatt acgtgtacta gcttttgctt accgtccact tgataatttt
     4921 gatttatcat ttgaagatga agacgatttg atccttgttg gtttgcttgc tatgatcgat
     4981 cctccacgtg ctgaagtgac tcaggcggta acagaagcaa gaagtgcagg aatcaaaaca
     5041 atcatgatta ctggtgacca caaaacaaca gctgttgcta tcgcaaaaca aatcgggatt
     5101 tttgaagaag gaaacctggc tttgaccggg acggaactag atgaattgac agaagatcaa
     5161 ctaaacgaag ttttagaaaa agtcactgtt tatgcacgtg tttcacctga gaataaaatt
     5221 cgtatcgttc gtgcttggca aaataaaggt catatctcag ctatgacagg tgatggtgta
     5281 aatgacgcac cagcattgaa acaagcagac atcggaattg ctatgggaac gggtacagat
     5341 gtggcaaaag atgcagcagc gatggtattg acggatgata actttgcttc tattgtcagc
     5401 gcagtagaag taggacgtaa tgtgtttgat aatatcaaga aagctatttc ttatctattt
     5461 gcaggaaatt taggtgcgat cattgcgatc atctttgcct tagttgctga ctggtccaat
     5521 ccatttacag cattacaact gttgttcatc aatttagtca atgattcact gccagcaatt
     5581 gcattaggta cagaaaaagc tgaacctgct acgatgaacc gcccgccaag agatccaaat
     5641 tcaggaatct ttacggacaa aacattgatt tctgtaacgt atcgtggtgt gctgattggg
     5701 attgctgtta tctttgctca atggcttggc atgcagcatt cacctgaatt cggtgtagcc
     5761 atgtcatttt caacattgat ttggtgtcgg acattgcaaa cattcccagc acgttcaaac
     5821 acacaaacag ctatccaagc agggttcttc tctaacagaa gtgtcttact agctgtagtt
     5881 gtatgtagcg cgctttactg cttaacaatg atcccaggac tgaaagaagt cttctctatc
     5941 ccatcagcat ttggattgac agaagtcttt acatgtatgg gaatcgcact tgtgacaatt
     6001 gttttgatgg aaatcactaa attgatcatt gtacgagtac aaggaaataa ataaaacgaa
     6061 tcaacgaggt tgtgagcctg ccgtttaatt ctgagtcaca aggtgcaatt tcaacaaaca
     6121 gcttctcatg tttgttgaat attgtgactt gtgaccgaag agttggcagg agaacaccgt
     6181 ttaaggaaga agttgtaaaa tcagcatttt gagctaatac tgagggtcat ctgattggac
     6241 actgtttatt cggaaagagg gactttgaca cgactttggt cacagtccct ttttttattt
     6301 atgatagacg gaaagccaat cattttatta ttcatcaaaa gagatagaat agataaaaga
     6361 ggagaatgat ttattccgaa aagtgaaaac atataacaaa aaattagagg aaatcaaatg
     6421 aatgaaaaag aatgggatga atttgctgaa gaatattatg aaatccagca agaatcgcaa
     6481 gtaactattg ctgaagatgt aaaaaattat ctgaaaacgg agaaagtcct gcctgctgaa
     6541 acagcagcag acgttgctgg aggatctggc agatttcttt ccttagcaaa agaaattaat
     6601 cgctatgatt tgatcgattt ttcttcagga atgctagaaa atgcaaaaaa agaagcagaa
     6661 aaaaatgagt tgccaaatgt ttcctttatc aaacagtcgt tagaagaatt tatccaatca
     6721 aatgagacat atgaaatggt ttttacagca gcaaatccag cattggtaca tcttattcaa
     6781 ttagaaaaat tgatgaaaaa gtcaagcaaa tggtgcgtga taatcagagt agtggaatca
     6841 atcgatgatg ttttttatcc tatcgaaaag caactaggaa tgatagaaga agatcctaat
     6901 acatcgccag ttgtaatgga tcaatttgaa acatacttaa ttgatgaagg attcaaagtg
     6961 aagaaaaaag attttactta ttttacacag gaatcaatta ctgaagaatt gatgaagcta
     7021 tactatgacg cgtacgcaaa agatcctgtt gtgaccaggt acattcgcaa actattcagt
     7081 gaaaaacgag aaatccttag tacgactaag ctgacctata gactgctcct ttggtccgca
     7141 gataaaaaaa gaacctttca cgactcgtga agggttcttt ttatgatttt attatatttt
     7201 gtagtaacga atatcatttt cgatctttgt aatcgaaaaa ggtacatctg aagtatattc
     7261 tgcacattct ccgtgttcag gaagcgtctc actgaaaatc gtttcatatt cgtcaacagt
     7321 gatccgtgtt cgagaatcta gcatctcttg atgagattgt gcgaagagat attcttgata
     7381 gttttcttcc agataacctg taaaaaattc acttactgca ccagaaccgt agctgaatag
     7441 accgatgcga tctcctggct gtagtgattt cgaattttcc agtagagaag ttagccccag
     7501 gtataatgaa ccagtgtaaa gattaccgat tcgtcggctg taacggatgc tttcttcata
     7561 gcgagccata agacgttcct gattttcttc gtctgtttgg tctaatacgc tttgcaatgc
     7621 cttttttccc atcttagtat acggaatatg gaaagcaatc gcctgataat cttcgagtcc
     7681 tcgacccgac aattctttat gtcgattcca aactttttgg aatgattcga tatacgtaga
     7741 attagataaa ggaccatcaa caacaggaaa ttcgctataa tctggacgcc agaaatcata
     7801 gatatcttct gtcagaaata cgctgtcgtc ttcaatcgat aaaatacgcg ggttttgagt
     7861 gatcatcatc gcaacagcac cgacaccttg cgtcacttca ccaccgcttg ccaagctgta
     7921 acgagcaata tcacttgcta tgactagtac ttttcgttct ggatgatttt tgacatattc
     7981 tttcgccatg tgcaaagctg cggtgccacc gtagcaagcc tcttttactt caaaagaacg
     8041 agcaaaagat tggattttca gtaaatggtg gataattacc gcactcgctt ttgaatgatc
     8101 aatcccggat tctgttccga cgatgaccat gtcgattagc tcacggtctt tttctgttac
     8161 gattttgctg gcagcatttg ctcctagtgt aatgatgtct tcatttgcac gattgactgc
     8221 catttggtct tgaccaatcc ctatatggta ttttgcagga tcatccccac ggctttctgc
     8281 caactctgtc atatctaaat ataaattagg aataaaaaag gaaagacgat caatccctat
     8341 tttcatttca acacctcttt acttatgata aaaaacttac tttactaagg tatcataaaa
     8401 agattaaaag aaatataaaa aagagaatat ttaaggattg ttaaaattta atcttagaaa
     8461 aaaataactg gaacaagagc tatttgtttt tacagcaaag aatggtatat ttttgtagga
     8521 ggtggcttac ttgaaagaag tcgttatgat agatgctgca agaacgccga taggaaagta
     8581 tcgcggaagt ttaagtccat ttactgcagt tgaattaggc acacttgtga ctaaaggatt
     8641 attagataaa acaaaactga aaaaagataa aatcgatcaa gtaatatttg gaaatgtcct
     8701 gcaagctggt aatggtcaaa atgttgccag acagattgct ttgaacagcg gtctccctgt
     8761 agatgtccca gcaatgacga tcaatgaagt ttgcggatct ggtatgaaag cagtgatctt
     8821 agcacgccag ctgatccaat taggtgaagc agaacttgtg atagccggcg gaacagagag
     8881 tatgtctcaa gcacctatgc tgaaacagta tcagtcagaa acaaatgaat atggtgaacc
     8941 aatttccagt atggtcaacg acggattgac tgacgcattt tcaaatgcac atatgggatt
     9001 aaccgcagag aatgttgcaa cacaattttc tgtgagcaga gaagagcagg atcgctatgc
     9061 cttgtcgtcc cagttgaaag cagcacatgc tgtcgaagcc ggtgtatttt ctgaggagat
     9121 catcccagtc aagatttctg atgaagacgt gttatctgag gatgaagcag ttcgtggaaa
     9181 tagtacattg gaaaaactgg gcacgttacg tacagtattc tcagaagaag gaactgtaac
     9241 agcaggaaat gcttccccgt tgaatgacgg tgcctctgtg gtgatccttg catccaaaga
     9301 atacgcagaa aataataatc tgccttattt agcaaccatc aaagaagtag cggaggtcgg
     9361 tattgaccca agtatcatgg gaatcgctcc aatcaaagcc attcaaaaat tgacagatag
     9421 atcaggaatg aatttatcaa caatcgatct ttttgagatc aatgaagcat ttgcagcttc
     9481 ttctatcgta gttagccaag aactgcagtt agatgaagaa aaggtcaata tatatggagg
     9541 agcaatcgca ttaggccatc caattggtgc aagcggtgca cgtatcttga ctacattagc
     9601 ttacggattg cttcgtgaac agaagagata cggtattgct tcattatgta ttggtggagg
     9661 tttaggcttg gcagtcttgt tagaagcgaa tatggagcaa acacataagg atgtgcaaaa
     9721 aaaaaagttt tatcagctta cgccatctga acggcgcagt caattaatag aaaaaaatgt
     9781 attgacacaa gaaactgcgt tgatttttca ggaacaaaca ctttctgaag agctctccga
     9841 tcacatgatc gaaaaccaag tgagtgaagt ggaaatccca atggggatcg cgcagaattt
     9901 tcaaatcaat ggcaagaaaa aatggattcc gatggcaaca gaggaaccat ctgtcattgc
     9961 agctgcaagt aatggtgcaa aaatctgtgg aaatatctgc gcagaaacac ctcagcggct
    10021 gatgcgaggg caaatcgttc tatcaggaaa atcggagtat caagcagtga tcaatgcagt
    10081 taatcatcga aaagaagagc tgattctttg tgcaaatgaa agttatcctt caatcgtgaa
    10141 acgcgggggc ggtgttcaag atatctctac tcgggaattc atggggagtt tccatgcata
    10201 cttatctatt gattttttag tggatgtaaa agatgcaatg ggcgctaaca tgatcaactc
    10261 gattcttgag agtgtggcaa acaagttacg ggagtggttc cctgaagaag agattctatt
    10321 cagtatttta agtaattttg ctacagaatc attggcttct gcctgttgtg aaatcccatt
    10381 cgaacgctta gggagaaaca aagaaatcgg tgaacaaatt gccaaaaaaa tccaacaggc
    10441 aggggaatat gcgaagcttg acccttaccg tgcagcaacg cacaacaaag ggatcatgaa
    10501 tggtatagaa gctgtagtgg cagctacagg aaatgataca cgtgcggtta gcgcttcgat
    10561 ccacgcctac gcggcaagaa atggattata ccaaggatta acagactggc agatcaaagg
    10621 ggataaacta gttggaaaac taacggtgcc tttggctgtt gccacagtag gcggagcttc
    10681 caatatcctg ccaaaagcaa aagcatcttt agctatgtta gatattgatt cagccaagga
    10741 actagcacaa gtaattgctg cagtagggtt agctcaaaac ctggctgctt tgcgtgcatt
    10801 agttacagaa ggaatccaaa aaggccacat gggacttcaa gctcgttcat tagcgatctc
    10861 aattggggct attggtgagg aaatcgagca agttgcgaaa aaattacgtg aagcagaaaa
    10921 gatgaaccag cagacagcta tacaaatatt ggaaaaaatc cgggaaaaat aaaaaagcaa
    10981 tatctttaga agaataaagc aagatttaca gttattttat ttactgcaga tcttgctttt
    11041 ttggctcttc gtcaaattgt gttggcgaag acaaatttga ataaaactta gtccgatatg
    11101 cggcgcgttc tgtgcgccgc atattttttt agttttcaaa gatcagacct tgttgtaaat
    11161 agatccgtgc ccgaaagtta taaaagttgc gataaccaaa cgcaattcgt ttaatcagct
    11221 tgatcttgtt attcagcccc tcagtgacgc cgtttgagtg gggcacctta aaggcatttg
    11281 aaatacccga ttggtagcga tggaagatcg tgaatttctt tttaaacgac tgaggtaacg
    11341 tagatgaaat tgttttaagg gtttcttgaa agccttccgt atctcgttta acgaaataat
    11401 agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc ttcgttgtag cttaacagct
    11461 catcaacgat ttctgtttga ctaattggtc gtttaaacag tcgatgataa tgccgtttga
    11521 gtggctccaa tgtcgtggaa tctttgagta agagtttcca atatcgcttg agccgacggt
    11581 acttcttctg attttcagag tgtgaggttc gaaaacgatt catgatctgg acacgtaatt
    11641 gattgaagct gcgattgatg tgttggacaa tatgaaagcg gtcagttacg atctccgcat
    11701 ttggaaagac tgttttaagc aactgacaat agctggcatt catgtccatc actaggtact
    11761 tgacctttaa acgggccttt ctaggaaagc gcaggaagta ttgagttagt ttgtaaagtc
    11821 tgcgatcttc aaggatcgta aatagctgat tggttgtccc atcaacacaa ataaaactca
    11881 tggaaccaga aacagacttc attgatttga actcatccat acacaacacc gtcggaagga
    11941 agttcatacg tggctggtaa tgcttcgttg cttcacgcaa gatccgtgtc accgtcccat
    12001 ccgagacgaa gtacttctta gcgattagtt tacgtgattg attttcgtac agttccatga
    12061 gaatcgcctg cttcagttcc tttgttagtt gatgatgttc atcaactaag tccgttttcg
    12121 cagaaaaggt ccgtccacaa tcatggcaga gaaaacgggt acgttttaat tctaaaatag
    12181 tcaacttttc tttgactttc aacatctgag t
//
LOCUS       DASJTM010000082        12128 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJTM010000082 DASJTM010000000
VERSION     DASJTM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.082"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            285..485
                     /locus_tag="U4B29_002195"
     CDS             285..485
                     /locus_tag="U4B29_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107130.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            619..1845
                     /locus_tag="U4B29_002196"
     CDS             619..1845
                     /locus_tag="U4B29_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107131.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2442..2837
                     /locus_tag="U4B29_002197"
                     /pseudo
     CDS             2442..2837
                     /locus_tag="U4B29_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            2915..3954
                     /locus_tag="U4B29_002198"
                     /pseudo
     CDS             2915..3954
                     /locus_tag="U4B29_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3967..4905
                     /locus_tag="U4B29_002199"
                     /pseudo
     CDS             3967..4905
                     /locus_tag="U4B29_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            4898..5245
                     /locus_tag="U4B29_002200"
     CDS             4898..5245
                     /locus_tag="U4B29_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107132.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5560..6417
                     /locus_tag="U4B29_002201"
     CDS             5560..6417
                     /locus_tag="U4B29_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN2107133.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            6437..6997
                     /locus_tag="U4B29_002202"
     CDS             6437..6997
                     /locus_tag="U4B29_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEN2107134.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            7886..8653
                     /locus_tag="U4B29_002203"
     CDS             7886..8653
                     /locus_tag="U4B29_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEN2107135.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            8679..9224
                     /locus_tag="U4B29_002204"
     CDS             8679..9224
                     /locus_tag="U4B29_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107136.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            9225..9791
                     /locus_tag="U4B29_002205"
     CDS             9225..9791
                     /locus_tag="U4B29_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107137.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            9806..10054
                     /locus_tag="U4B29_002206"
     CDS             9806..10054
                     /locus_tag="U4B29_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107138.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            10073..10312
                     /locus_tag="U4B29_002207"
     CDS             10073..10312
                     /locus_tag="U4B29_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107139.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            10329..10913
                     /locus_tag="U4B29_002208"
     CDS             10329..10913
                     /locus_tag="U4B29_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107140.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            11020..11301
                     /locus_tag="U4B29_002209"
     CDS             11020..11301
                     /locus_tag="U4B29_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2107141.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            11291..11632
                     /locus_tag="U4B29_002210"
     CDS             11291..11632
                     /locus_tag="U4B29_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2107142.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         4523 a         1668 c         2456 g         3481 t
ORIGIN      
        1 atgatgaatc agcaagtagc gataagcttt tgattgaatt agtatcaagt cttagtagtt
       61 ttggaaaaat gaaaagattt aatgtgatat tagaaatttt tgcttggtta atcccttcat
      121 ttatttggtt tttttatatt ttcatagttg aatctgagtt aatagccaag gtctctcttg
      181 gactgttaat tccgatataa ataggttcag caatttactt taataacttg tatacaccta
      241 ttcaaataaa aaagcagtta ttacatgaag gattaagtgg taatatgtta aaaagaattg
      301 catgggataa agataataaa acagatattg agcttaaaga atataaatac aattatttct
      361 cgagatgtat agatagaaag ttactgttta aaaaagaagg attagaattt agttttgatg
      421 aagatgaatt tgaaaaatta gtgatggact ggcgtaagaa tttactggat acggtacaat
      481 tctaaaagca attgaggtcg aaattccgat acttgaatca tgatctatat tatcgtgtta
      541 tgagtgaaat ttctaataaa gataagatat ttcatttgga cagtcatgtg aatatgagct
      601 taacaattaa acgtatttat gatcttttat atgtttataa acattaatga ggtgctaaaa
      661 ttgacaacaa taactatgac aaaaaaaata gaagtatatg ttgatggatc acaagaagaa
      721 agggattcct tttttgattt tttttggaaa aatagtagag aatatgtaaa agcgttgaat
      781 ctggtatata aacagctaat gtttgaagag ttgataaaag atgcaattaa agaaatggat
      841 caaggttatg ctgagagaga agaaagtatt caaaatcgga ttgaaaagtt agcatatact
      901 gatccaatag atcaaaaaaa acttgataaa gcaaaagctg aattatctaa attgagaaat
      961 gaaaaaaata aagaagcgag agaaatatta acgcaagcga tagggttgaa gagtcaaacg
     1021 agaacacgag atgtagcaaa aaaattagac tttgactttg gagattttgt tgattccgca
     1081 aatatgaaag ctagtaaaga ttttggcaat gattttatcg gaattgtgac cggacaaaga
     1141 acgcctagaa tatataaaaa tagaaacaaa tatggattat taccttttag aggtcgagat
     1201 attaaaatcc ataaagatga taagttttat atttatttac ctaaaggctt taggtttaaa
     1261 gtgaatttag gtagcaagcc aagaaaagca agagatgttg cttcagtttt acaacacatt
     1321 atggatggcg attacaaatt aaatcaaagt aatcttattt ttgatggaag aaaattattt
     1381 tttaatctaa caatttcttt tgaaaaaagc tataatgaaa tggaattcaa gaaaggtaat
     1441 gaattgatcg taaactttgc agatttgtgt agcggtgaat gtttaattaa tgataaatct
     1501 gtaatgtctt ttggtgatag ccaatatata agaaacttca aaagtaaaat tttagcaatg
     1561 aaatcaaaag aacaaagccg aggagtcttt tctaagggcg gtcatggtcg aaaaagaaaa
     1621 ttagatcaag atcgatggga agcaataaag aaaagagaaa gtaattttgt taaaacttat
     1681 aataatcaat gttctgcaaa aatcgttaaa actgctattc gcaataaagc tcaaattatt
     1741 cgtatcattg aaccagataa ggcttatggc gaatgggctt actatcaatt gaaagaacaa
     1801 ctagtaaaca aaggaaaaag atatgggata gaagtcagaa gttagtaaat taaacaatcg
     1861 ttgatttggc aacgaaatag tgagggaaaa tccactcact gaagggtaaa ctccgtcaag
     1921 ttgtgtgacc agacggaagg acgaatgttg ttgaggtttt agaaccatat aaaacagcat
     1981 gtttctcaaa tttttaggat aagggaaatt gctagcagtt tgttttagaa ccatataaaa
     2041 cagcatgttt ctcaaatgag tactttaaat taattgatgg cgttcctgct ttagaaccat
     2101 ataaaacaac atggatttaa aacaagaagt aagtcagttc tccttttact ttagtaagga
     2161 aaaataaagt attctcaata aaacttaaaa taagcaaacc tttgttttaa aatgattgtc
     2221 aagttaaaaa aatctgggat catataaaat agtatagttc tcaaatatga ttagaaaccc
     2281 tattaaagag caatattaag ccaacttact taaatcgttt aattaagaac aataatctct
     2341 tttagtcaga cttttagtct gacttttttg tatttccaaa atttattctt tacaaaaacg
     2401 aacatacgtt tgtataatta cttgtgaatg ggggaataaa aatgtttgat tacagtagag
     2461 aaccggtgaa tgactatttt ttaatcgaca tgaagtcttt ctatgctagc gtggaatgta
     2521 ttgaacgaaa tttagaccca atgactacac cgttaattgt catgagtaga tcggacaaca
     2581 ctggttcagg tctgactcta gcttcttcac ctactgcaaa gaaaaaatat catattacta
     2641 atgttagtag agctactgat ttaccttatc cattaccaaa agaacttcat attgttccac
     2701 cacgaatgaa cttatatata aaaaaaaata tggaagttaa tcagattttt agaaagtatg
     2761 tcgcagatga agatttattg atttattcga ttgatgaatc aattttaaaa gtgacaaaat
     2821 ccttaaattt atttactata taaattgagt taaattgtat tcgtttttcc tcctataggc
     2881 gtatactaaa gaaaacactt gaggagtggg gaacatggaa acagaacgcc aaatccgtga
     2941 attggaaaaa gcgatgaata cgacagacaa ccgccggttg tacgagcgtt tcctggcagt
     3001 caagttggtt cttgaaggcc acacccggaa agaagccggg cacaccatcg gccgcgacga
     3061 gcatactgtc agccactatg tgaaaaacta ctgccataat ggaatccaag gtttagtctc
     3121 caaaaaacaa agcggacgac cttcacggct gacagatgaa caggaagcgg aactggtcca
     3181 aatcgtcgcc taccacactc cggaggaagt aggtttcaaa tcccgtgcca attggacatt
     3241 ggcgattgtg tgtgacctga ttctccggga atggagttat ggatatacgc aaaaaggcgt
     3301 ggcggacctc cttcaccggc taggactaag ctggacccgt ccgacgtata ccctcaaaaa
     3361 agcggattcc aagaaacaac aggtattttt ggaagaaacc ttcccgagcc taaaaaaaaa
     3421 ttgctgaata gcgatattga ccacctccta ttcgaggacg aaaccatgat ccgggattac
     3481 caagccattg gctgcacctg gtttctccgc ggccaccagc gaaaaatccc cacttatgga
     3541 caacacaaag gggtaaaatt gataggcacc ttggattatg aaaccggtcg gatcatttgt
     3601 acagaagaag gggcatacga cgcagcggcc ttcctccgtt ttctggagcg gacgttgcgg
     3661 gaatacccca ccggaaaaat cgtgatggta ttggataatg cccttatcca ccacgccaag
     3721 ctgatccagt cttttcttga agaaaacgct aggcgcctgg cactcatctt cctccctccc
     3781 tacagccctc agttgaacct aatggaaggc gtttggaagt ggctcaagga atcggtcatc
     3841 aacaatgttt tttttgacca cgtccagaaa atcaaacagt cggtccgcgg tttcttagcc
     3901 gatgtaaacg agcgtcccct tgaggtgatt gaccgactgt gcgtgcggat gtaaagtatt
     3961 taattcaacc tatatagttc aggaacaaga agcgagagaa gaagagaatt agctaaaata
     4021 atcaaagatg agattcagaa agaaacaggt ctaatagcaa cagtcggaat aggagataac
     4081 ccattattag ctaaacttgc actggacaat gaagcgaaga gtagtaaaga atttatagct
     4141 gagtggactt atgagactgt acctgaaaaa atatggaaaa taaacgagat gactgacttt
     4201 tggggcattg gatcacgcat gaaaaaaaga ttgaatcata tgggaattat gagtatagaa
     4261 gatttagcaa attggaatcc ctacacaatt aaaagtcagt taggtgtaat cggactccag
     4321 ttatatttcc ataccaatgg aattgatcgt acggatattg ctattccacc agaaccaacc
     4381 aaagaaaagt cttatgggaa ttcacaagtt ctaccaaaag attatacgag gagagaagaa
     4441 gtagaactag tagtcaagga gatggcagag caagtagcca ttcgaattag acaacacaat
     4501 tgcaaaactg gttgtgtacg cttagcaatt ggtacgtcaa ttttggaaga aaaatcaggt
     4561 ttctcgcacc agatgaaaat accaattaca gataatacga aagaattaca aaattattgt
     4621 ctttttttat tcaataagtt ttatgagggg caggaagtac gacatatagg agtaacttat
     4681 agcagattag tatacacaga ttccctacaa ttagatttat ttagtgatcc aagaaaaaag
     4741 attgctgaag aaaatttgga taggattatt gataagatta gacaaaaata tggatttaca
     4801 gctattatac atgcttcatc tatgttagaa ggtgcaagaa gtattacgag aagcacttta
     4861 ataggaggac acgcaggagg attaggaggt ttgcataatg attgaatata acgatagagg
     4921 acgtttaaaa tggtcgggat tttatttgtc tgatcatact gaaagaatca atgcagacaa
     4981 tgtacaacgt gctaatcata atttggcaaa agaacaaatg acgactgaag agattgcgaa
     5041 aatactgaga actgctgtca taaaaaatag acccgtctgc attcaaaaag aggaacgaaa
     5101 cgctgaaggt tactatccag atgatgtagt aggaaaaatt attggatatg acgagcttgg
     5161 tgtatttgtg gaagaagaaa aagttcacta tgatgaaatt aggaatgttt ctttctcgga
     5221 attattaaaa tggagtcaga tatgaacatt attaaaaaat acgaattcgg ttatattacg
     5281 tatgaacagc taatagagga attatgggga tatggtcaac aattaattaa tgaagtgggg
     5341 attgattgct tttgtttcta tattgaatcc ggagcaggat accataagaa cagatactat
     5401 atatttccat atccaccaaa atagtctctt agatattttt aaattttctc caaatacttt
     5461 caattattgt gaaatatttc cctttaatta atcaaatttt ttataaaagt aatatataaa
     5521 ataaattgct tgaagtatta tatttagata tcataattta tggaagaaaa attaattaaa
     5581 aaggatgaga ttataatggg gttactggtt ccgagagcta atacttatac tataccttta
     5641 acgaaacgcc aattaattgc taaaagatat caaaggatta ctagagccat caatagagag
     5701 ttttggaatt ctgaaagtga tactgctcac agtttatacg taggttctta tgggagagga
     5761 acggcaatta gtactagtga tatagatata atagttgaat taccgatggc agaatttgac
     5821 agattcaaaa attatttatc aaatggcccg tctaagttat tacaagttat aaaaaacgca
     5881 tttcaggaaa tactaccaaa tagtgatatt agagctgatg gacaagttgt taaaataaat
     5941 ttccatgatg gaatcaaatt tgaaatagtt ccagctttta atgaaaaaga ttattggggt
     6001 gagtcaaaag ggtttattta tccagattca aacatgggag ggaactggaa agctacaaat
     6061 cccaaaaaag agcaagaagc catgaaatta aagaatacaa aaagtaataa tttattatat
     6121 gctacgtgta aacattttcg acatgtacga gatacagaat ttacgagtta tcatctttca
     6181 ggaatagtaa ttgacagttt cgtatatgag gctatgggaa attggaaatt cgttgaaaat
     6241 aacagtgggg gtcaaaacat tagttctgta tcttatgaga cagctttatt agaatactat
     6301 aactcacata aagttatggg cggtttaaat ttatactctc ctggaagcaa tcaatttgtt
     6361 aattctgata gtagtatcat atgtttagaa aaagtattaa aaaaaatagc tttataaagt
     6421 tttatggagg aacaacatgg gagacgaaaa aaagcgcttg gaagttcaat tacgtgaagc
     6481 atatggacga gtaacgtata cttacaccac tcatttaaag caagccgata atattatttc
     6541 tgacaataaa attttaaggt gtcttcaaat tggtttgtca gcccttacta ctggaggggt
     6601 acttagtacc attattactg ataatagtga gttaaccttt gttgctggat tatgttcaac
     6661 aatttctctt ggattaaact tatactttaa gaattttgat cttgctgaag aagctaaaaa
     6721 tcatcaaatt gttgccgata aattatggtt gatcagagaa gaatacgtaa gcttactaac
     6781 agatttaaat cgtttaagtg ttgaagatat tgtgaaaaaa agagatcaat taatgaacga
     6841 aactcatgaa gcatataaaa atgcccctaa aacaaatagt aaaagctatg aatcagctca
     6901 gaaagcatta aaatctgaag aagagcaatt ttttacagaa gaggaattgg ataagatgtt
     6961 accagagcat cttagaaaaa agagttctaa atattgaact ctttttttat gcttaaaatt
     7021 ctcttgtaat ttttctattc atctatattt aagaagaaat atgatgatag aaacatctcc
     7081 taaagccacc gcaagccgtt ctaagcatag cttagcccga cattgctaga tatgctcatc
     7141 tcaccatgaa cgtttcctaa agccaccgca agccgtccta gccattgcct agtccgacat
     7201 cgctagatta gtgcatctcg ccataaaagt tttctaaagc caccgcaagc cgttctaagc
     7261 atagcttagc ccgacattgc tagatatgct catctcacca tgaacgtttc ctaaagccac
     7321 cgcaaaccgt cctagccatc gcctagtccg attttgcttg atcggttcca tttctgttta
     7381 tttagtgggt caacaccgta atcttaaata gccatcgcct atttaagatt actaggtctg
     7441 ctttgaaggt aaaaagtctt tcattcttca agacttttag gctttcaagc tacgcctgaa
     7501 agcattgaaa ttcatcgctc tttatttggt tttgccattt ataattcctt acgcccatta
     7561 tctttttcgc ctgtcatacc ttgtcagaag tgtttagagc ttatctcatc tttggattaa
     7621 tacaagtggc gtaaattttt ttctaaacgc aagcgttacg aaaataattt acttaaacac
     7681 ttgtattaat ccggcagata agaacgcatt ctaatcactc ccgacaaggc aagacaggcg
     7741 aaaaagataa ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa atagcgatga
     7801 agaaaaaacc acctccctct aaaacaatcc aaacaaaaaa gaattttgaa aaaccaaaaa
     7861 tcaaaaacag aaagaagcgt tcaacatgag agatagaatt ttagaagtta atcaagaaat
     7921 ggtaaacaag ttaacaaata tcagtgggaa ctatgaaaag tacgatggta ctttacttga
     7981 taactatatt tttcacgata cacaaaatat cagaattggc agaagtaaac cgagagcgtt
     8041 cattctcatc aaagagaaat atttgaatga atggaatagc acctatgaaa tggttatgac
     8101 tgataacttt gaaaaggtat tggatttcag aaatgctttg ttgggttctg atatgggaaa
     8161 tagcagtctt gaatctattg aagttgaaaa caagaccgag aatgaaggtg aaggattgat
     8221 taagaatgtc ttgaaaatcg aacaaaaata tatcagtgaa gatagaagtt tttatagagt
     8281 cacaagctcg gttggtctag caaactttgc tactaactta attggaaaca atgctagtga
     8341 atctttgcta gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata gcgaagtttt
     8401 caaaggtgga attaatcaat cagtagcgat accaagagag atttttcaac tagctttgct
     8461 aaacaattca gcaaaaatcg cagtatcaca taatcatccg agcggatatg aatttccaag
     8521 tgaaaatgac aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa ttggtattga
     8581 attgttagac catatcatcg taacaagtaa aaacacaagc tattactcat ttcaagaaaa
     8641 tggttcttta taaaaattag agaaaagagg aaaacaagat gggcgaaaga acacaagcgt
     8701 taattacagt caaatatggt aacagcaaaa cgaaagcaag cgtgtcgatt cattatcagt
     8761 gggggtatgg tcgatatatg ctgatggacg tattgaatca aggaatttat ttaatcaata
     8821 aagcatacga cttacgagat aaatcaagag aagaattagc aaaaggaatc tttgatagaa
     8881 gtacaggagt tatcaatttt gtcacctatg aatctggagt gagtgcttat gatgaaacag
     8941 ggtttggtgg gcgctatgaa ccttatcaag aaacgattga agatgtgatg aagttttcag
     9001 ataacaatga tggctatgct catttagaga tggtgttcga tgaaaatgga tttagtctag
     9061 atgataaaac cagattgcga ttgtttgatt ttgagggtca acagatagga ctaaacaagt
     9121 tttgtgaaag cggaaaagag tttgctacga aagattttat taagggatac agattattgc
     9181 tagaaaatta tggaataaac ctagaagaag agagcgtgaa ataagtgttt gaaaaaagaa
     9241 aagaccagtt ccctaattta gaagcgaaaa gtcaaaaagt gattgatggt gaaatgattg
     9301 aattattcca aaaaagttat ggaaccatag aaactaccgc aagttgcaaa tactactttc
     9361 aaggaaagag aaacacgttt aaaggctttg ccgattctga tgaagaattg gcaaagaaaa
     9421 tcattgattg gcttgtcagc agtgaacacg tagcaagaga agataccgtt agaacaacga
     9481 ttaatgggga agtcctaaca ggtactagat gtgtttgttg taatcatgaa tttgtagaag
     9541 atgaaggttg ctaccgaatc aatgaatatg gtgattttat ttgccagagt tgccacgaga
     9601 tagaatttcc aaccgaaaga agttggtttg aatacatgct acaagattat gagatggaaa
     9661 cagaaaatgg tgaagtagtt ttttgtacca aagaggaatt gagtaagaga agcgatgatg
     9721 aaattgaaga ttggtgtgtg tggttagtag ggacagatga ttgcgttgca tattacacaa
     9781 cagcagaata ggagtgtgaa tcagcatgga aggaatggat tttatagatc atgaagattt
     9841 gattgatttt ggttatacat ggaaaggaat ggtaggtatt agcagaagtc ttgctaatgc
     9901 cttttacgaa agaaattatg cagtctatgt cctttatgat gacaatacag aaagccttgt
     9961 tgatgaagag tataaattgg atttagagaa tgttttatat gggatagaaa aagaagattt
    10021 agcaaaatat attttttcat ggttgggtca ataaacgaaa gagggtagca acatgacaaa
    10081 attcaaaagt aaaggtatgc aatggtgttc ttattattat gttgggaatg atgaagagct
    10141 tttgagaaaa gccaaacaaa aaggttatcc agataagatg ttaaaacaga ttggctatta
    10201 catgccagaa gagagaatag aaaaagaatc ttgtaaggtt ctttttgata gtgaacagct
    10261 attagttttg ttaactcaaa ataaattgat ttgtcaattt gaaaataatt aatatggggt
    10321 gaagaaccat gaatgagaat aagatggtat taagtgaata cgcaagattg agaaccatta
    10381 attggttgaa tggtatgcaa agtagtggcg cttttgatgg atttgatgag gagaatatgg
    10441 actttgaaag tttatcgaat gaggatatta gatattattt tatcgaatgg tattttacag
    10501 aaccagatga agaggtggaa gatgaaccag aattaatttt agaacgtcaa agaattttat
    10561 tcagtgaaca agatagtaag aggttgtatg gtttatatga cttacaactg aaaggattaa
    10621 aagcgcagta tgttgctgat agttacgatg atatggtaga acagggggca gatagggttg
    10681 tcacttattc agaagatgat gaagaggaat gggaaaacta ttcagatgat gagatatgct
    10741 ttaatgatgg gtatatgcct attgaaatca ccgaagaaga atatgataaa gtgtcggata
    10801 gcgatgagat tggtatgtgt acggttgtaa gtggattgga tatagatgtc caaagagtag
    10861 aggttgcata ccagaagatg aaagaacgta taaaaagtaa agtagaatct tgatttgatt
    10921 ggattgtttg caaagtagct aattaagagt ataatataat cataaatgta gttaatggat
    10981 ataataatga gtatattata ctcaaaagag gtgaacgaaa tgaaggtaaa aaatccaaaa
    11041 gaaaaaagca gaattcaagt cggaatagac aaggatttaa aagaaaacgc agaaatgata
    11101 ttggaagagc tagggttaaa tccaacaaca gcaatcacaa tcctttataa gcaagtggtc
    11161 gctagaggtg agtttccagt agagataaag ctaagcgaag aggaaaagca aggaatcaga
    11221 ttgcaacagc taactatgga tatgccagtt gatgttctag atacagatga aaagttagaa
    11281 gagtggttca atgaagccta atgagatagt aacggtctat gttgcattta cagataaaaa
    11341 tggtggtaaa agaagaccta ttttggtggt tagtgataaa gaagatagag tagaattttt
    11401 tggaatcaca agtcaatatg agaataaatc agaccaaata aagagagtgt attttcctat
    11461 tgcagaatgg gaaaaagtag gtctaaagaa gcaatcatgg attgacgtag ggtctttaaa
    11521 ggctattccc aaaagccgag agaacatatc atttaaaaag gttggtatgc tatcagctat
    11581 tgatacgaaa aagctaaata ggtttatcga gagattacaa agaaaagtct aacaaggctt
    11641 ttcttttttt gtttatatgt atattaagat atacgtttat ataaacgtat ttatgtatat
    11701 taaaatacac gtaaatatat acgtgattat aaaaattaag aaaatattaa gtattgttaa
    11761 atagtgataa ttagtaatat acgaattata agatacattt tatatttaat gtatgtaaat
    11821 aaataaaatg actgacggat attgaatagt aataaataac aataattcag acggatacag
    11881 tgagtgaagt agcttgctac gaacgaacag gcagaatgaa acggtagttt cattagagca
    11941 agtcgcttgc gacgtagcgt taccactgat gtaactggca agttacatag agagagtttg
    12001 cttgcaaacg aacgttaccc acttgaatag aaaacaaaaa taacatatta gtgaattttg
    12061 aataggagcc acaactcaac caaccgcttg cggtgggtga gtcccacgga ggaaaagacg
    12121 aacgaacg
//
LOCUS       DASJTM010000083        12123 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJTM010000083 DASJTM010000000
VERSION     DASJTM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.083"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(81..638)
                     /gene="frr"
                     /locus_tag="U4B29_002211"
     CDS             complement(81..638)
                     /gene="frr"
                     /locus_tag="U4B29_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN2107143.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(641..1363)
                     /locus_tag="U4B29_002212"
     CDS             complement(641..1363)
                     /locus_tag="U4B29_002212"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN2107144.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1499..2380)
                     /locus_tag="U4B29_002213"
     CDS             complement(1499..2380)
                     /locus_tag="U4B29_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN2107145.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="U4B29_002214"
     CDS             complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="U4B29_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN2107146.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3620..4099
                     /locus_tag="U4B29_002215"
     CDS             3620..4099
                     /locus_tag="U4B29_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN2107147.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4316..5407
                     /gene="phnW"
                     /locus_tag="U4B29_002216"
     CDS             4316..5407
                     /gene="phnW"
                     /locus_tag="U4B29_002216"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN2107148.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5400..5528
                     /locus_tag="U4B29_002217"
     CDS             5400..5528
                     /locus_tag="U4B29_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2107149.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5532..6077
                     /locus_tag="U4B29_002218"
     CDS             5532..6077
                     /locus_tag="U4B29_002218"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2107150.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6531..8222)
                     /locus_tag="U4B29_002219"
     CDS             complement(6531..8222)
                     /locus_tag="U4B29_002219"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN2107151.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8642..9589)
                     /gene="arcC"
                     /locus_tag="U4B29_002220"
     CDS             complement(8642..9589)
                     /gene="arcC"
                     /locus_tag="U4B29_002220"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN2107152.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9704..10723)
                     /gene="argF"
                     /locus_tag="U4B29_002221"
     CDS             complement(9704..10723)
                     /gene="argF"
                     /locus_tag="U4B29_002221"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN2107153.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10814..12043)
                     /gene="arcA"
                     /locus_tag="U4B29_002222"
     CDS             complement(10814..12043)
                     /gene="arcA"
                     /locus_tag="U4B29_002222"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN2107154.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2519 c         2197 g         4093 t
ORIGIN      
        1 ttttttaatt tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc
       61 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
      121 ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      181 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      241 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      301 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      361 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      421 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      481 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      541 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      601 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      661 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      721 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      781 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      841 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      901 cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      961 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
     1021 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1081 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1141 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1201 cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1261 tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc
     1321 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1381 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1441 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1501 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1561 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1621 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1681 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1741 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1801 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1861 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1921 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1981 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     2041 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2101 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2161 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2221 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2281 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2341 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2401 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2461 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2521 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2581 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2641 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2701 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2761 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2821 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2881 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2941 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     3001 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3061 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3121 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3181 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3241 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3301 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3361 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3421 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3481 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3541 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3601 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3661 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3721 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3781 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3841 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3901 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3961 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     4021 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4081 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4141 tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt
     4201 attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4261 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4321 taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4381 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4441 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag
     4501 ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4561 ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4621 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4681 gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4741 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat
     4801 agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga
     4861 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4921 tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct
     4981 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     5041 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5101 aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat
     5161 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5221 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt
     5281 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5341 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5401 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5461 ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact gacaaagaaa
     5521 tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5581 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5641 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5701 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5761 cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt
     5821 attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt
     5881 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5941 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     6001 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6061 ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat
     6121 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga
     6181 atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac
     6241 agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6301 agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6361 gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6421 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6481 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6541 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6601 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6661 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6721 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6781 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6841 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6901 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6961 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     7021 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7081 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7141 ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7201 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7261 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7321 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7381 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7441 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7501 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg
     7561 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7621 gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca
     7681 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7741 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7801 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7861 gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct
     7921 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7981 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     8041 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8101 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc
     8161 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8221 atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8281 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8341 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8401 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8461 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8521 tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aaaaatccag atggcctaaa
     8581 ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa
     8641 attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta
     8701 aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta
     8761 ctttcggaag catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg
     8821 aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt
     8881 ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag
     8941 caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag
     9001 gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga
     9061 cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc
     9121 cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt
     9181 ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca
     9241 caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt
     9301 cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat
     9361 ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac
     9421 cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca
     9481 ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag
     9541 acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc
     9601 tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc
     9661 ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa
     9721 acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt
     9781 ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga
     9841 tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca
     9901 agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga
     9961 tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata
    10021 cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa
    10081 tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt
    10141 caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga
    10201 ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt
    10261 ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc
    10321 attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca
    10381 ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg
    10441 tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat
    10501 gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt
    10561 caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca
    10621 agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt
    10681 tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac attctccttt
    10741 agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc
    10801 gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac
    10861 caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac
    10921 gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg
    10981 ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac
    11041 cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt
    11101 tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct
    11161 taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa
    11221 atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt
    11281 caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag
    11341 ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt
    11401 cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg
    11461 gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca
    11521 tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt
    11581 ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc
    11641 tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt
    11701 gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat
    11761 gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt
    11821 ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa
    11881 agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat
    11941 ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct
    12001 ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc
    12061 tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat
    12121 tca
//
LOCUS       DASJTM010000084        11994 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJTM010000084 DASJTM010000000
VERSION     DASJTM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.084"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="U4B29_002223"
     CDS             140..460
                     /locus_tag="U4B29_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107155.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="U4B29_002224"
     CDS             912..1112
                     /locus_tag="U4B29_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2107156.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="U4B29_002225"
     CDS             1259..4048
                     /locus_tag="U4B29_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2107157.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="U4B29_002226"
     CDS             4095..4622
                     /locus_tag="U4B29_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN2107158.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="U4B29_002227"
     CDS             4643..5833
                     /locus_tag="U4B29_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2107159.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="U4B29_002228"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="U4B29_002228"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN2107160.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="U4B29_002229"
     CDS             7966..8640
                     /locus_tag="U4B29_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2107161.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="U4B29_002230"
     CDS             8637..8978
                     /locus_tag="U4B29_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107162.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="U4B29_002231"
     CDS             9008..9367
                     /locus_tag="U4B29_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN2107163.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="U4B29_002232"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="U4B29_002232"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2107164.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         3997 a         2101 c         2591 g         3305 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatg
//
LOCUS       DASJTM010000085        11803 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJTM010000085 DASJTM010000000
VERSION     DASJTM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.085"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            606..812
                     /locus_tag="U4B29_002233"
     CDS             606..812
                     /locus_tag="U4B29_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN2107165.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1014..2015)
                     /locus_tag="U4B29_002234"
     CDS             complement(1014..2015)
                     /locus_tag="U4B29_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2107166.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2020..3933)
                     /locus_tag="U4B29_002235"
     CDS             complement(2020..3933)
                     /locus_tag="U4B29_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2107167.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(4101..4607)
                     /locus_tag="U4B29_002236"
     CDS             complement(4101..4607)
                     /locus_tag="U4B29_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2107168.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4767..5207
                     /locus_tag="U4B29_002237"
     CDS             4767..5207
                     /locus_tag="U4B29_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2107169.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5233..6390)
                     /locus_tag="U4B29_002238"
     CDS             complement(5233..6390)
                     /locus_tag="U4B29_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107170.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSVKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6393..6749)
                     /locus_tag="U4B29_002239"
     CDS             complement(6393..6749)
                     /locus_tag="U4B29_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107171.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7047..8021)
                     /gene="sgrA"
                     /locus_tag="U4B29_002240"
     CDS             complement(7047..8021)
                     /gene="sgrA"
                     /locus_tag="U4B29_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN2107172.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8217..9056)
                     /locus_tag="U4B29_002241"
     CDS             complement(8217..9056)
                     /locus_tag="U4B29_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2107173.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9241..10128
                     /locus_tag="U4B29_002242"
     CDS             9241..10128
                     /locus_tag="U4B29_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2107174.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10722..11417)
                     /locus_tag="U4B29_002243"
     CDS             complement(10722..11417)
                     /locus_tag="U4B29_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN2107175.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11401..11799)
                     /locus_tag="U4B29_002244"
     CDS             complement(11401..11799)
                     /locus_tag="U4B29_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2107176.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3514 a         2331 c         1779 g         4179 t
ORIGIN      
        1 agccactacc aaatatttgg tagtggcttt tttggttctg caagggtggt acagaacatc
       61 aatagtcggt gggttagaac gtatgtcatc gcatctttgg agaaggttgt attggtatta
      121 ttcgagttac ttgttagatg cgataacaaa gttagtatac agtagtaaaa aatttatctt
      181 ttcttttttt tattattttt gtttcttatt ttctaaaaaa ttaaaaaaaa ttttggtgaa
      241 ttaagtattt ccttgataac actggctttt ggcctcattt taggttatca tttgtaattt
      301 tatttcacag ttgatatgat tgttatgtaa aataaaaaag tttaggatac atcgaatttt
      361 tggggaaatt cttttggggg tatcctaaat cttttctatt atattcccat attttctatt
      421 attggtctag acgtatgatg gtaaactttg tacctatctg attgctgaat cattaattgc
      481 ttatcattag ccatctttgt ttcaagttcg ctagatgatc attttattca catcctaaca
      541 aacttgtcac atttatagat tcttaatcta tttatcatac aattataaaa aaggaggtgc
      601 tgcttatgaa agctatccat atttctttgc tttttttcgc tagtgcattg attttatttg
      661 ctattcatct ttgttcccag ccttatatca ataacaacgg aatattagtg gaaccagcat
      721 tcttttgctt accactcggc tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg
      781 ctttttttaa acgatcaaaa caacaaatct gacttataaa atttacgtaa aagagatcgt
      841 gacaataatc gtgtgatatt cccaaatttc gaataaaagg tgccccttgc catctctaac
      901 tcctcagtca caagttacaa tattcataaa ccatgaaaag cttgtttatt gaaattgctc
      961 cttgtgactt ggagctaagt gacagattac aacctctttt gcatcataag actttactcc
     1021 ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac
     1081 tgatattcat ggttcaaaac agcatttttc tccgcataat ctacttttgt gactttcacc
     1141 cccgctttct caaatacaga agttacttct tccatttgtt tcgtaaacgt atttgtattt
     1201 ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt
     1261 ttgaccttct ctgattgttc aaagtcctta ttatcaaagt aagcccatcc acatttttca
     1321 taaagaaagc tagagatttt atcacttact tgagtaagtg cagaaggatc caacagacca
     1381 gacaaagaaa taaacttcgt aatctgcttg ttctccaaag tcggatcaag atgatcagct
     1441 tttttaattt cttcattttg aagtgctaac aaatattctc cactaatttg tgcacctgcc
     1501 gaatttccac caatcgaaat attcgaccaa tctgcttgat cttttatctc ttttttagct
     1561 gcttgaatgg cttcgttcaa ttggataact ggtgtaggat gttttgcttc cggtgatcgt
     1621 tcataatcta tatttacgat gatttgctgc gtgtctgaac atagatattc caaataatca
     1681 cgacaatcat ttttatcccc tcctacatat gcgcctccat gcatccagaa aagaatcggt
     1741 aattttttct tttccacttt cggtgtgtag atatccatat tattattttt gtactttgaa
     1801 ggataggata tttctttttt tatatcgatt tgttcgattt tttgctggaa atcatctggt
     1861 cgggcatata cttgttcttt ttcacttgtg tcaaattgtt ttctaacaag caagctaatc
     1921 ggctttggtg tgatttgatt aatcaaaaat acagtgacta aaataactac gatgataact
     1981 ggtactgcaa tcactattcg cttgaaccat ttcattcatt taccctctct ttcttcgcca
     2041 aatccatgtg ataatcgtgc taacagataa aaatccacct gaataaatga gaagcgaatg
     2101 tttcttttct ccagttttcg gcaacattct tttttttgca tctgtctttt tttgtacttt
     2161 cactgtcatc tgaatcggtt gcgtatcatc acttgattga tcctctcgag gttcattctt
     2221 tcccttattg tctgcattct ctgtcggatc ttgctttttt ggtggagcta ctgggatttt
     2281 tttgtaatag aaaataatag ttggtatcgt ctcattaaaa atcccttcag tcggtccttc
     2341 aacatttgct aattcgtatc cttcgaatgt cttttgctct gtcatataat tttctccata
     2401 agctccgtaa agctggattt cgtcctggtc tttgagtaga cttttattgt tttcatccac
     2461 atactttacg tttatctgat catggaacac tactttcaaa aagtccgtta catgtttcgt
     2521 gtaggcagca tggtcaactt cctgtgcctt tccatgccct gctccttcta ctaataaaag
     2581 atctttatac cctcctgata actcatataa tttttgcgcg ttagacacag gtactacatc
     2641 atctgctgtt ccatggataa tcaatttagg caatgaatct gacattccaa tcgtaggtag
     2701 agcagctttt gtatccaaat gcatctcctg atcaaaatag tattgtttca aaaggcccat
     2761 aaataaatct tcatgctctt gatccccaat gatgtctaac tgatttttta tggcatccgg
     2821 tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa
     2881 tccgcaattt tcaataattc ctttgacatt gacaggaata tctttcgcta atacagacat
     2941 catcgtagct gctcccatcg aaccaccata aagtaaaatc ttttgagatg gccgctctcg
     3001 aacttcttga tttatccagt aaaggacgtc atctgactca tattgtccat aggtcacata
     3061 atcgcctccg ctattaccag tcgctctgga atcaacaaat aataaattat acccggcctt
     3121 ataaaaatca ttgtattctt ctgtaacgat cccgttattc caattccctc gaaatccgcc
     3181 atggatcatg actgttttat ctgaaccttg atcgacataa tatgcatata catccatcat
     3241 tttgccatct tgtttgttta gtacagtttg atgaagttgt tttgtatttg gatagaagcc
     3301 tgtgttttct gtaggcgttg tcccatcatc tgtacctcca agcatcgaca tcaacacact
     3361 gttttttgca aaagcgatat cgtacaatag ataagtagca atcagatgtt tgataatctc
     3421 tttatctaca ccataatcat taacagcaag ctcagtgact aataactcta ttaattttga
     3481 cttgatgtct tctgttggtc cattaagtat ttctgtcatc tgtttaccga agtaattcaa
     3541 atattcttta ccattgaaat tttcaccttt accgatatca tcattttctg tcccattctc
     3601 tttgtcactt actttagcag aagaggattc gtcgtttgac atttttgtag ctgttgcttc
     3661 ttctgtttgt tggttatctg tggtagcggt cgtttcttga tctgtgctag taacagatgt
     3721 ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc
     3781 agcatcagta gtttctgtgg tttctacctc ccgagattcc gattcggaag aaatgctctc
     3841 tccatatact gtccattgcg ggacgcttgt acttacagca aataagatga ccaacgctcc
     3901 ctttaatact tgttttgttc tcaagctatt cactcctttt tatgtttcta ggagaattat
     3961 atgtaaacgc aaacattttt tatacaaaaa ataaatggtt tatcccataa aaatagaaca
     4021 ggattatttt ctcattttac gtaataaagc agaatggata aaccagtatc cattctgctt
     4081 tttttaaacg aagaaacaac ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt
     4141 ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag gattatcata agttgtcgtt
     4201 gctcctctac acatcataag ctggtatcct aatccatgag caaacttcac agtcgtatct
     4261 atacaatact ctgcttgtgc cccacataat tcgatcgaat gacagtccaa ttcagttaat
     4321 aaaacactca aattagtctt ataaaatgaa ttcgcatggg tttttctcac aaaataactg
     4381 tgctcaggaa tagataattc cgacaatagc tcccatggtt ttgtttgtgg cactaattga
     4441 gcatcttcgt gttgaacgaa aataactggt ttcccaatcg tttcgtatgt tttgattctt
     4501 ttattcacta atgctgtcac tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc
     4561 cctttttgta aatcgatcac tactattgcg tcggctgtgt ttgacatact tgtgccttct
     4621 ttcttttttc ttgatttaat agtatttctt atctagctag ttccattata cgtgacaaca
     4681 ttttcttatt ttacacctct aaatggcact tttcgactat ttcttcatgt tattatttca
     4741 taaatactta gctattggag gaatgtatga tgattaaatt gtatacaggt tctggctgct
     4801 cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga tataccgttt atcgaacaga
     4861 acttaattcg tcaaccacta accaaaaatg aatttatcca cttactaaaa ttgacaaaca
     4921 acggcgtgac cgaaatgctc tctactcatt ctattagtta taaaaatctt gttcacagaa
     4981 tagatgaact cagtttgagt gaactatata ttgagattcg tacatcccct tctttattga
     5041 aaaaaccgat tatctacgac caatctgcaa aacttttgac tggatttagc gaagataaaa
     5101 tcaggatgtt cctttctaaa actaaaagaa agcaagagct caacgaactc ttgctttctg
     5161 gtcatcttgt cgattttaaa gaaaataatc tttttgcaat gtcttaattt tggcataaac
     5221 aatctgcttt tcttaattaa cgaaatccaa tttttccaat atggatctat ttttctcatt
     5281 cctgattttt atgatttcag ctttcagacg cgcccaatca ttttttgtag gttgtccatt
     5341 ccagtaaaat atctgtgtat tcgtttgatc atatgccttt ttgtcgatag gataatcgct
     5401 aatgacaata tcaggtaact gatcagcgga tgcactatat tttattgggc gttcagtcat
     5461 agataatatc cttttctttg cccataattc ctgtgtttta ccatactttg attgaatata
     5521 gaatatgaca ggttgttcgt gttttataaa aatatccctg actaacatac agtattgaaa
     5581 aatcaaccgt ctgttctttt cataataagt attaagctct ggaattctgc gagctacccc
     5641 actaaaaaac tgttgcatga ttggatacat cactggaaat atatgctgaa aatctctgtc
     5701 ttctgctgat tttccaaaag catccacctt cttttttgtc ccaaaaaccc ttgaatacaa
     5761 atggatcatt gccaaattga catatagata atttttttct gaagttgtaa aacaaatctt
     5821 gaagtttttt tcaaaatttt ctacaataga cgattcaatc cagccccaac tttcttgtac
     5881 tttatctgga gtatccaaca aatcttcttc caaataggtg ttcattacac caaacataaa
     5941 ataaaggtaa gcttgctctg ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa
     6001 aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt atcaatggat gcacaaattt
     6061 gttcatctct tctgggacat acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat
     6121 tcttagtagg ctgatccatt tctttctatc tatactacgc gtatatggcg catgtttcgc
     6181 aaatttttct aatagttgac gtttattttt tacacttgat aatatatcag aatgattttc
     6241 gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg atacggtatt cttctccttc
     6301 tatcggatta ccttttttca agctgcaaga taaatgatag tttttcaaaa tagtattaag
     6361 ttctcttaat tttctttgaa tcgtacgcaa acttatataa ttttttgact aagattttct
     6421 aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat cgttccctct
     6481 aaaagtatag aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc
     6541 ttgttttctg aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct
     6601 tgttgaatca ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc
     6661 accacatata atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt
     6721 tcattgatat caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga
     6781 cttattccga cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat
     6841 actaccatgc gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct
     6901 gttatttcct gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact
     6961 tcgccaggca atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc
     7021 aattttagat ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag
     7081 cttgctccta caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt
     7141 ctactaccag tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga
     7201 tgatcaattc cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct
     7261 gcatttctct tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact
     7321 tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct
     7381 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta
     7441 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca
     7501 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     7561 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     7621 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     7681 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     7741 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     7801 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     7861 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     7921 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     7981 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     8041 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     8101 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     8161 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     8221 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     8281 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     8341 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     8401 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     8461 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     8521 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     8581 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     8641 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     8701 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     8761 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     8821 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     8881 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     8941 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     9001 acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     9061 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     9121 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     9181 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     9241 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     9301 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     9361 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     9421 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     9481 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     9541 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     9601 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     9661 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     9721 aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga
     9781 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     9841 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     9901 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     9961 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
    10021 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
    10081 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
    10141 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
    10201 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
    10261 ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga
    10321 atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc
    10381 ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa
    10441 aatatccctt ctggacttgt tcttcttatt ctgttatact agagaaacac gaaagatatt
    10501 tttatgcgac acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga
    10561 gttacaagga tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt
    10621 tagtaatagt cacccaaact ttaaattaat gaatattgaa tcgtcagata gcctcctcga
    10681 gttttcgaga aggctatcgt ttattttatg gagaaattga ttcagaagta atcagcgacc
    10741 ttttttagtt caccaatcgt ttctgcttgg aaagtttgca cccaatcttc ttggaagccc
    10801 ctcactgctt tatcaatcga gggcatctct aatacttgat ctagtagctc tttacctaac
    10861 gttatcgcat gagctccaga tcgaatggct gtagtgactt gttcacaatt tttgaaacta
    10921 gctgctaata tttttgttgg tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc
    10981 actttcatag catcaatgcc ttgattttgc atcctgttat aataaggtgc aatataatct
    11041 gctccattat taatagctaa atacccttgt aattctgtgt aaatagctgt accggtaatc
    11101 aaataacctt cttttttcaa ctttttgatc gcttgaaggc ctgcttgatt caccggcacc
    11161 ttgataaacg tatcttttcc cagttcttct atgataactc gagcatcctc tatcatttct
    11221 tcttccgttc ttcctattgt ctgaatatgg ataggaatct gtccgattat ttttttgatt
    11281 tctttcatat gcgagaagaa gtttgacgct ccttcttttt tcagaattgt aggatttgtt
    11341 gttacgcctg ttaaattcaa aaactgttga caagaataaa tactttttgt atttgctgta
    11401 tcaagcatga tttccatata gctttccccc attctgatcc tcttgatcga ttttaatgat
    11461 tgtccccttt cttaaatgcg gcatttcctt atcttctaca tacattgttc ctggaagttc
    11521 cggctcatga gatccatcaa attttatcgt gatatgtcct aattcattca aattagtttg
    11581 gacaacatct cctactgctg tgattacata catttgttta tcaaaacaga gcttcatatc
    11641 acaagttatt tcttttctta cctgattcaa ttcaatctta taacaataat ctgttaatgt
    11701 ttccggtgct tcatctccaa ataaaatcat tattttttct ttcctgaata cttctgcttc
    11761 tataccaaca tctgttacaa ccgtttcaaa gatcgtcatc tct
//
LOCUS       DASJTM010000086        11674 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJTM010000086 DASJTM010000000
VERSION     DASJTM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.086"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B29_002245"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B29_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2107177.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U4B29_002246"
     CDS             complement(323..892)
                     /locus_tag="U4B29_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN2107178.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U4B29_002247"
     CDS             1078..1980
                     /locus_tag="U4B29_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2107179.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B29_002248"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B29_002248"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN2107180.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B29_002249"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B29_002249"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN2107181.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U4B29_002250"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U4B29_002250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN2107182.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="U4B29_002251"
     CDS             8554..8739
                     /locus_tag="U4B29_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN2107183.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="U4B29_002252"
     CDS             complement(8801..9997)
                     /locus_tag="U4B29_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2107184.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="U4B29_002253"
     CDS             complement(10167..11603)
                     /locus_tag="U4B29_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2107185.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3449 a         2487 c         2048 g         3690 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaa
//
LOCUS       DASJTM010000087        11647 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJTM010000087 DASJTM010000000
VERSION     DASJTM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.087"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..579
                     /locus_tag="U4B29_002254"
     CDS             16..579
                     /locus_tag="U4B29_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107186.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(640..1005)
                     /locus_tag="U4B29_002255"
     CDS             complement(640..1005)
                     /locus_tag="U4B29_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107187.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1081..1326)
                     /locus_tag="U4B29_002256"
     CDS             complement(1081..1326)
                     /locus_tag="U4B29_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107188.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1396..1650)
                     /locus_tag="U4B29_002257"
     CDS             complement(1396..1650)
                     /locus_tag="U4B29_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107189.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1814..2137)
                     /locus_tag="U4B29_002258"
     CDS             complement(1814..2137)
                     /locus_tag="U4B29_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107190.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2324..2397
                     /locus_tag="U4B29_002259"
     tRNA            2324..2397
                     /locus_tag="U4B29_002259"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2358..2360,aa:Ile,seq:cat)
     gene            complement(2782..3279)
                     /locus_tag="U4B29_002260"
     CDS             complement(2782..3279)
                     /locus_tag="U4B29_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2107191.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3319..4128)
                     /locus_tag="U4B29_002261"
     CDS             complement(3319..4128)
                     /locus_tag="U4B29_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2107192.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4462..5319
                     /locus_tag="U4B29_002262"
     CDS             4462..5319
                     /locus_tag="U4B29_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2107193.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5312..6214
                     /locus_tag="U4B29_002263"
     CDS             5312..6214
                     /locus_tag="U4B29_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2107194.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6247..6597
                     /locus_tag="U4B29_002264"
     CDS             6247..6597
                     /locus_tag="U4B29_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2107195.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6646..7569
                     /locus_tag="U4B29_002265"
     CDS             6646..7569
                     /locus_tag="U4B29_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2107196.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7647..8540
                     /locus_tag="U4B29_002266"
     CDS             7647..8540
                     /locus_tag="U4B29_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2107197.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8578..9141
                     /gene="amaP"
                     /locus_tag="U4B29_002267"
     CDS             8578..9141
                     /gene="amaP"
                     /locus_tag="U4B29_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2107198.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9155..9343
                     /locus_tag="U4B29_002268"
     CDS             9155..9343
                     /locus_tag="U4B29_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2107199.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9356..10258
                     /locus_tag="U4B29_002269"
     CDS             9356..10258
                     /locus_tag="U4B29_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2107200.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10290..10532
                     /locus_tag="U4B29_002270"
     CDS             10290..10532
                     /locus_tag="U4B29_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2107201.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10788..11159)
                     /locus_tag="U4B29_002271"
     CDS             complement(10788..11159)
                     /locus_tag="U4B29_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107202.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4149 a         1980 c         2201 g         3317 t
ORIGIN      
        1 tttttaaaaa taactgtgtt atatattaaa aaaggcataa tagttataag cttttttaaa
       61 gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt tattatttta
      121 gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg
      181 ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact
      241 gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat
      301 cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga
      361 aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt
      421 gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt
      481 atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag
      541 gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt
      601 taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt
      661 ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg
      721 cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat
      781 aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag
      841 ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg
      901 tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg
      961 ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt
     1021 atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa
     1081 tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta
     1141 aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt
     1201 acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg
     1261 gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat
     1321 actcatttaa tcacctttca ttatagtaga atagttataa tgactataga aagctacaca
     1381 tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa
     1441 taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt
     1501 gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa
     1561 ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac
     1621 ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata
     1681 aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata
     1741 gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct
     1801 gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat
     1861 ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg
     1921 aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga
     1981 gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa
     2041 ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata
     2101 aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca
     2161 ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac acaagtaaaa
     2221 gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag tttgtcattc
     2281 gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt tagctcagtt
     2341 ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa
     2401 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
     2461 ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag
     2521 gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt
     2581 tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta
     2641 agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga
     2701 tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat
     2761 cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag
     2821 ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc
     2881 tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc
     2941 aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat
     3001 ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc
     3061 ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag
     3121 ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa
     3181 tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg
     3241 atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat
     3301 atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc
     3361 attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc
     3421 aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat
     3481 gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt
     3541 gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc
     3601 tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa
     3661 tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt
     3721 ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt
     3781 cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata
     3841 gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat
     3901 ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat
     3961 tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac
     4021 gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc
     4081 atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta
     4141 ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat
     4201 gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg
     4261 ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt
     4321 agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga aaacaaagca
     4381 tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt tagaaaaaga
     4441 ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt ggtaactgct
     4501 tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt agaaaattgc
     4561 aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga atatagtaaa
     4621 gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca attcctgaca
     4681 gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac taccgcttac
     4741 ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt cctttactta
     4801 gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc gttaaatgga
     4861 aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag attaggaatc
     4921 gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga tcaatggaat
     4981 aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga tcgaatcatc
     5041 tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca acttcatcca
     5101 aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat
     5161 gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac aggacatgat
     5221 aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa aaaattagca
     5281 cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag cagtagtcgc
     5341 aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag tattagataa
     5401 ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg attttgaaag
     5461 taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg tggaattctt
     5521 gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc
     5581 ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac gtatcttata
     5641 tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg
     5701 tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg aaaaaatgaa
     5761 aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa
     5821 atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata atccagaaaa
     5881 attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt ggttaccact
     5941 tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac atccaacagc
     6001 agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc atttttcagg
     6061 acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt acgaaaaata
     6121 tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa aatatgcaaa
     6181 agttcaacca aaaacagatg tagttgttaa ataatagaag acaacaactg aagaggggga
     6241 gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga aaaggttggg
     6301 gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct aagggacaaa
     6361 ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa
     6421 tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat
     6481 tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa
     6541 aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag
     6601 tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag
     6661 aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc
     6721 gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc
     6781 gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca
     6841 ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg
     6901 ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa
     6961 tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc
     7021 acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag
     7081 cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa
     7141 caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa
     7201 acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta
     7261 ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat
     7321 ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc
     7381 atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt
     7441 tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta
     7501 gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat
     7561 acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt tggcaataca
     7621 atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac ccgcgaaact
     7681 tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta cagcagaata
     7741 tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa ctagaaaacc
     7801 gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg attgcatttg
     7861 ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa gatgcagagg
     7921 aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt cttactacct tttgatcttc
     7981 gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt ggcgggctag
     8041 atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct gagttaccac
     8101 ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct ttagtgaaag
     8161 aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct gttcaagcat
     8221 tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc gctaacttta
     8281 cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt gtagcacctg
     8341 gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg atccctgaat
     8401 ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca ccagtttatg
     8461 ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga gtaactggtg
     8521 gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg
     8581 aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc tgtgcttttg
     8641 tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa gtttcgattc
     8701 ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct
     8761 attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat cggaaacttt
     8821 ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga aggaatggtt
     8881 cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt caaagcaacg
     8941 aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc actcattggt
     9001 aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt gggaacaaaa
     9061 gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa acgaagcaat
     9121 gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt cgacatacaa
     9181 gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga tcaccgtggg
     9241 ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta
     9301 tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa catccatgaa
     9361 taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc
     9421 agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc
     9481 agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag aagagaagaa
     9541 acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta aagttccaga
     9601 acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc cttctgacca
     9661 tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa aacttggttc
     9721 tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa tgcgcggtga
     9781 gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga
     9841 cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac
     9901 agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag cagttgatat
     9961 ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt
    10021 aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca acgctaacgt
    10081 agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc aagacaaagt
    10141 aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata aagtaaaatc
    10201 agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg ttcagtaata
    10261 aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt atcgtcggag
    10321 gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg gggattattg
    10381 caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta ggttcttggg
    10441 gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc attttagtac
    10501 taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa
    10561 agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
    10621 ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg gaataagagg
    10681 ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca attagatttt
    10741 taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta ataaaacaat
    10801 gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac gaccactttg
    10861 aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa gcaatggatc
    10921 agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt tactcttgtc
    10981 tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag taaacaaagt
    11041 ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag caggaaaaat
    11101 gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat aaatggcata
    11161 ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt tgtttgtgaa
    11221 gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa tcattattat
    11281 caaaataatg ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca
    11341 aaactacata gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat
    11401 atatgggagc ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa
    11461 gttttaagca cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac
    11521 tgctcaaact gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa
    11581 ctacaacgta acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag
    11641 gccccct
//
LOCUS       DASJTM010000088        11264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJTM010000088 DASJTM010000000
VERSION     DASJTM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.088"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..1485
                     /gene="lysA"
                     /locus_tag="U4B29_002272"
     CDS             226..1485
                     /gene="lysA"
                     /locus_tag="U4B29_002272"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN2107203.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1472..2272
                     /gene="dapF"
                     /locus_tag="U4B29_002273"
     CDS             1472..2272
                     /gene="dapF"
                     /locus_tag="U4B29_002273"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN2107204.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2275..3156
                     /locus_tag="U4B29_002274"
     CDS             2275..3156
                     /locus_tag="U4B29_002274"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN2107205.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3153..3869
                     /locus_tag="U4B29_002275"
     CDS             3153..3869
                     /locus_tag="U4B29_002275"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN2107206.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3875..4570
                     /gene="dapD"
                     /locus_tag="U4B29_002276"
     CDS             3875..4570
                     /gene="dapD"
                     /locus_tag="U4B29_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN2107207.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4586..5587
                     /locus_tag="U4B29_002277"
     CDS             4586..5587
                     /locus_tag="U4B29_002277"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN2107208.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5584..6945
                     /locus_tag="U4B29_002278"
     CDS             5584..6945
                     /locus_tag="U4B29_002278"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN2107209.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6905..8056
                     /locus_tag="U4B29_002279"
     CDS             6905..8056
                     /locus_tag="U4B29_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN2107210.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8031..9200
                     /locus_tag="U4B29_002280"
     CDS             8031..9200
                     /locus_tag="U4B29_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2107211.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9375..10754
                     /locus_tag="U4B29_002281"
     CDS             9375..10754
                     /locus_tag="U4B29_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2107212.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11035..11264
                     /locus_tag="U4B29_002282"
                     /pseudo
     CDS             11035..11264
                     /locus_tag="U4B29_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3749 a         1958 c         2508 g         3049 t
ORIGIN      
        1 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
       61 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
      121 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
      181 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
      241 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
      301 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
      361 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
      421 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
      481 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
      541 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
      601 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg
      661 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
      721 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
      781 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
      841 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
      901 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
      961 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     1021 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     1081 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     1141 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     1201 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     1261 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     1321 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     1381 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     1441 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     1501 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     1561 tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt
     1621 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     1681 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     1741 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     1801 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     1861 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     1921 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     1981 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     2041 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     2101 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     2161 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     2221 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     2281 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     2341 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     2401 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     2461 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     2521 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     2581 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     2641 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     2701 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     2761 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     2821 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     2881 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     2941 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     3001 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     3061 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     3121 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     3181 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     3241 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     3301 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     3361 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     3421 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     3481 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     3541 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     3601 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     3661 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     3721 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     3781 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     3841 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     3901 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     3961 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     4021 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     4081 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     4141 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     4201 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     4261 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     4321 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     4381 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     4441 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     4501 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     4561 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     4621 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     4681 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     4741 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     4801 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     4861 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     4921 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     4981 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     5041 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     5101 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     5161 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg
     5221 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
     5281 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
     5341 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
     5401 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
     5461 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
     5521 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
     5581 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
     5641 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
     5701 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
     5761 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
     5821 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
     5881 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
     5941 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
     6001 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
     6061 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
     6121 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
     6181 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
     6241 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
     6301 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
     6361 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg
     6421 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
     6481 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
     6541 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
     6601 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
     6661 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
     6721 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
     6781 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
     6841 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
     6901 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
     6961 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga
     7021 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
     7081 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
     7141 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
     7201 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
     7261 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
     7321 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
     7381 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
     7441 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
     7501 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
     7561 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
     7621 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
     7681 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
     7741 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
     7801 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
     7861 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
     7921 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
     7981 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
     8041 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
     8101 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
     8161 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
     8221 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
     8281 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
     8341 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
     8401 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
     8461 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
     8521 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
     8581 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
     8641 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
     8701 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
     8761 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
     8821 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
     8881 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
     8941 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
     9001 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
     9061 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
     9121 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
     9181 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
     9241 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
     9301 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
     9361 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
     9421 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
     9481 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
     9541 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
     9601 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
     9661 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
     9721 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
     9781 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
     9841 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
     9901 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
     9961 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    10021 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    10081 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    10141 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    10201 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    10261 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    10321 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    10381 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    10441 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    10501 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    10561 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    10621 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    10681 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    10741 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    10801 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    10861 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    10921 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    10981 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    11041 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    11101 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    11161 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    11221 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DASJTM010000089        11197 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJTM010000089 DASJTM010000000
VERSION     DASJTM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.089"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..906)
                     /locus_tag="U4B29_002283"
     CDS             complement(13..906)
                     /locus_tag="U4B29_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107213.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U4B29_002284"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U4B29_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN2107214.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="U4B29_002285"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="U4B29_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN2107215.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U4B29_002286"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U4B29_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN2107216.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U4B29_002287"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U4B29_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN2107217.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="U4B29_002288"
     CDS             complement(6343..7062)
                     /locus_tag="U4B29_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN2107218.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="U4B29_002289"
     CDS             complement(7059..8372)
                     /locus_tag="U4B29_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2107219.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="U4B29_002290"
     CDS             complement(8453..8773)
                     /locus_tag="U4B29_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107220.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="U4B29_002291"
     CDS             complement(8881..9168)
                     /locus_tag="U4B29_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107221.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="U4B29_002292"
     CDS             9295..9939
                     /locus_tag="U4B29_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN2107222.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="U4B29_002293"
     CDS             complement(9998..10705)
                     /locus_tag="U4B29_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107223.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="U4B29_002294"
     CDS             complement(10925..11095)
                     /locus_tag="U4B29_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107224.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DASJTM010000090        10936 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJTM010000090 DASJTM010000000
VERSION     DASJTM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.090"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..154
                     /locus_tag="U4B29_002295"
     CDS             <1..154
                     /locus_tag="U4B29_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2107225.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="U4B29_002296"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="U4B29_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="U4B29_002297"
     CDS             complement(1613..2455)
                     /locus_tag="U4B29_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2107226.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="U4B29_002298"
     CDS             2780..3196
                     /locus_tag="U4B29_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2107227.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="U4B29_002299"
     CDS             3193..3663
                     /locus_tag="U4B29_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107228.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="U4B29_002300"
     CDS             3677..4450
                     /locus_tag="U4B29_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107229.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="U4B29_002301"
     CDS             4464..5276
                     /locus_tag="U4B29_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2107230.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="U4B29_002302"
     CDS             5330..6295
                     /locus_tag="U4B29_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2107231.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="U4B29_002303"
     CDS             6431..9154
                     /locus_tag="U4B29_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2107232.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="U4B29_002304"
     CDS             9167..9799
                     /locus_tag="U4B29_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2107233.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10004..10231
                     /locus_tag="U4B29_002305"
     CDS             10004..10231
                     /locus_tag="U4B29_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN2107234.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10300..10566
                     /locus_tag="U4B29_002306"
     CDS             10300..10566
                     /locus_tag="U4B29_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2107235.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="U4B29_002307"
     CDS             10556..10825
                     /locus_tag="U4B29_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2107236.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3625 a         1742 c         2137 g         3432 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct ttttta
//
LOCUS       DASJTM010000091        10865 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJTM010000091 DASJTM010000000
VERSION     DASJTM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.091"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..1557)
                     /locus_tag="U4B29_002308"
     CDS             complement(46..1557)
                     /locus_tag="U4B29_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2107237.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /locus_tag="U4B29_002309"
     CDS             complement(1879..3231)
                     /locus_tag="U4B29_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN2107238.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="U4B29_002310"
     CDS             complement(3384..4460)
                     /locus_tag="U4B29_002310"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN2107239.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="U4B29_002311"
     CDS             4656..6401
                     /locus_tag="U4B29_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN2107240.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="U4B29_002312"
     CDS             complement(6681..7118)
                     /locus_tag="U4B29_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN2107241.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="U4B29_002313"
     CDS             complement(7102..8475)
                     /locus_tag="U4B29_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107242.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="U4B29_002314"
     CDS             complement(8797..9285)
                     /locus_tag="U4B29_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2107243.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="U4B29_002315"
     CDS             complement(9307..9909)
                     /locus_tag="U4B29_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2107244.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(9978..>10865)
                     /locus_tag="U4B29_002316"
     CDS             complement(9978..>10865)
                     /locus_tag="U4B29_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN2107245.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         3166 a         2269 c         1918 g         3512 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc cttatgaact
     9961 gaaccccaaa agttgaacta tttaatggac tgtttccgat attctactgg agatagtcca
    10021 tttaacttta gttttatcct tttgttatta taccacctaa tatactcatt taatttactt
    10081 tgaaatatct caattgatcg gaatttttct cgataaaaaa actctgattt aagcacacca
    10141 aaaaaatttt ctattacaga attatctaag caatttcctt ttctagacat gctttgaata
    10201 atgttattct cttttaattt tttttgatat tgtggcatct gatactgcca tccttgatct
    10261 gaatgtagaa tcagtgaacc ctcagttccc ttttttttaa ttgcttgttg aagcatttct
    10321 tcaatcagtt tatatgttgg actagttgat atactataac taattatttc tccgttaaat
    10381 aaatcaagta taggagatag atagattttt cttcctttta tcttgaattc agtgacgtct
    10441 gtcacccatt ttttattggg tgtatctact gaaaaatttc gttttaacac attcttggca
    10501 atttttccta ctgttccttt ataagattta tatcgcttta ttcggatttg acaggtaagt
    10561 cccatttggg acattaattt tctaactgtt ttatgattga ttgtatatcc tttcatcttt
    10621 aacgctaaag tgactctacg ataaccataa gagtttcttg attcttttac aatcgctgta
    10681 atttcttgct ttatcttgct atatttatct ggcttatcta atttttttac ccagtaataa
    10741 taagttgact tcgctaattg tgcaattgaa agtaatagat tcaatttaaa ttcttttttg
    10801 agctgaagga ttgttttaac cttgatttct tcttgctcaa atcttgttct tgaatcaagg
    10861 tttct
//
LOCUS       DASJTM010000092        10517 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJTM010000092 DASJTM010000000
VERSION     DASJTM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.092"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..306)
                     /locus_tag="U4B29_002317"
     CDS             complement(133..306)
                     /locus_tag="U4B29_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2107246.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="U4B29_002318"
                     /pseudo
     CDS             754..3116
                     /locus_tag="U4B29_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="U4B29_002319"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="U4B29_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN2107247.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="U4B29_002320"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="U4B29_002320"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN2107248.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="U4B29_002321"
     CDS             5704..6489
                     /locus_tag="U4B29_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2107249.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="U4B29_002322"
     CDS             6502..7353
                     /locus_tag="U4B29_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN2107250.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="U4B29_002323"
     CDS             complement(7477..7929)
                     /locus_tag="U4B29_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107251.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="U4B29_002324"
     CDS             complement(8394..8762)
                     /locus_tag="U4B29_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN2107252.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="U4B29_002325"
     CDS             8912..9394
                     /locus_tag="U4B29_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN2107253.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="U4B29_002326"
     CDS             9431..10441
                     /locus_tag="U4B29_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN2107254.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DASJTM010000093        10166 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJTM010000093 DASJTM010000000
VERSION     DASJTM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.093"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..84
                     /locus_tag="U4B29_002327"
     tRNA            12..84
                     /locus_tag="U4B29_002327"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="U4B29_002328"
     tRNA            88..160
                     /locus_tag="U4B29_002328"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="U4B29_002329"
     tRNA            168..249
                     /locus_tag="U4B29_002329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="U4B29_002330"
     tRNA            267..339
                     /locus_tag="U4B29_002330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="U4B29_002331"
     tRNA            351..422
                     /locus_tag="U4B29_002331"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="U4B29_002332"
     tRNA            439..524
                     /locus_tag="U4B29_002332"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="U4B29_002333"
     tRNA            535..608
                     /locus_tag="U4B29_002333"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="U4B29_002334"
     tRNA            618..691
                     /locus_tag="U4B29_002334"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="U4B29_002335"
     tRNA            711..783
                     /locus_tag="U4B29_002335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="U4B29_002336"
     tRNA            811..884
                     /locus_tag="U4B29_002336"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="U4B29_002337"
     tRNA            898..971
                     /locus_tag="U4B29_002337"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="U4B29_002338"
     tRNA            995..1084
                     /locus_tag="U4B29_002338"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="U4B29_002339"
     tRNA            1097..1170
                     /locus_tag="U4B29_002339"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1261
                     /locus_tag="U4B29_002340"
     tRNA            1189..1261
                     /locus_tag="U4B29_002340"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1265..1337
                     /locus_tag="U4B29_002341"
     tRNA            1265..1337
                     /locus_tag="U4B29_002341"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Phe,seq:gaa)
     gene            1342..1412
                     /locus_tag="U4B29_002342"
     tRNA            1342..1412
                     /locus_tag="U4B29_002342"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Gly,seq:tcc)
     gene            1425..1498
                     /locus_tag="U4B29_002343"
     tRNA            1425..1498
                     /locus_tag="U4B29_002343"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1459..1461,aa:Ile,seq:gat)
     gene            1510..1581
                     /locus_tag="U4B29_002344"
     tRNA            1510..1581
                     /locus_tag="U4B29_002344"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1543..1545,aa:Glu,seq:ttc)
     regulatory      1770..1849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1847..3040
                     /locus_tag="U4B29_002345"
     CDS             1847..3040
                     /locus_tag="U4B29_002345"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN2107255.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3170..4642
                     /locus_tag="U4B29_002346"
     CDS             3170..4642
                     /locus_tag="U4B29_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2107256.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4725..5513
                     /locus_tag="U4B29_002347"
     CDS             4725..5513
                     /locus_tag="U4B29_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN2107257.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5580..6248)
                     /locus_tag="U4B29_002348"
     CDS             complement(5580..6248)
                     /locus_tag="U4B29_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN2107258.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6363..8075
                     /locus_tag="U4B29_002349"
     CDS             6363..8075
                     /locus_tag="U4B29_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107259.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8078..9850
                     /locus_tag="U4B29_002350"
     CDS             8078..9850
                     /locus_tag="U4B29_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107260.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9932..>10166)
                     /locus_tag="U4B29_002351"
     CDS             complement(9932..>10166)
                     /locus_tag="U4B29_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2107261.1"
                     /translation="YNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDR
                     VGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2959 a         1799 c         2318 g         3090 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacattgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gtttggctcg gtagctcagt tggtagagca atggattgaa gctccatgtg tcggcagttc
     1321 gattctgtcc cgcgccatta tgcgggtgta gtttagtggt aaaaccacag ccttccaagc
     1381 tgttgtcgcg agttcgattc tcgtcacccg cttttagttt ttatgggcct atagctcagc
     1441 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1501 tattttattg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
     1561 gttcgaatcc cgtacgggtc atggaagata ctttttgagt atctttttct ttttatctga
     1621 aagaatgatt ttaaaaagct tagcagagtt atctttatgg atgattgtgc taagcttttt
     1681 tggacagttc taatgtaaaa tatttttata ttttttaagc aaaaaaatga aaaaatacga
     1741 gcagaaaact gttttatctg ctatacttgg ttcgttgcaa gttcccgaaa ggatccgcag
     1801 gacggagatg ccttgtaacc gaatttaact agggggaatc attataatgg tagaaagaca
     1861 cttatttaca tcagaatctg tttctgaagg acatccagat aaaattgctg accaaatcag
     1921 tgatgcaatc ttggatgcaa ttttaaaaca agatccaaca gcacgagtag cgtgtgaaac
     1981 gtctgtaaca actggtcttg ttttagtatt tggagaaata tctacgacgg catatgtaga
     2041 tattcaaaaa gtcgtacggg aaacaataaa agaaattggt tatacacgtg caaaattcgg
     2101 atttgatgga gatacagcag ctgtattggt tgcgattgat gaacaatctc ctgatattgc
     2161 tcaaggagtt gatgaagcac ttgagatccg ggatgaagat aaaaaagacg tattagatga
     2221 gattggtgct ggtgaccaag gtttaatgtt tgggtttgct gtagatgaaa caccagaatt
     2281 gatgccttta ccaatcgctt tgagtcatcg tctggtacga cgcttggcag atttgcgtaa
     2341 atcaaatgaa ttaacgtatt tacgtccaga tgcaaaatct caagtaacgg ttgaatatga
     2401 tgatcaagga caaccggaac gcgtagatac aatcgttatt tcaacacagc atgatgatgc
     2461 agtagataat gaaacaattc gtcatgatgt catcgaaaaa gtagtgaagg aagttattcc
     2521 agctgaatta ttagatgatc aaacgaaata ttatatcaat ccgactggcc gatttgtcat
     2581 tggtggtcct caaggggatg ccggattaac aggaagaaaa atcattgttg atacgtatgg
     2641 cggttatgct cgtcatggtg gcggtgcttt ttctggtaaa gatgcgacaa aagttgaccg
     2701 ttctgccagc tatgctgcac gttatattgc taaaaacatt gttgcagcag ggcttgctcg
     2761 caaagcagaa gtacagctag cttatgcgat tggtgttgct caacctgttt cgatctcaat
     2821 caatactttc ggtactggaa cggttccgga agaagaactg attgctgcag tgagagaaaa
     2881 cttcgatctt cggccagcag gaatcatcga gatgcttgat ttgcgtcgtc cgatttacaa
     2941 acaaacagct gcttacggtc attttggtcg tacagatgta gatttgcctt gggaacatac
     3001 ggacaaagta gatgcgttga aagcaagttt agcaaaataa gttatattca gctggaacga
     3061 tcgccaagtg gtggttgttc cagctttttt tatggcagtt aacctcttga aaaagatagt
     3121 aattatttat ctatttttag atcctgtcat aatgaaaggg aggaataaga tgaaaaaaga
     3181 aacaaatgtt actttggtca cagtgagtat tttcattgca acgttcatga cggctattga
     3241 aggtacaatc gtaacaaccg ctatgccaac gattgtcggt tcattacatg gaatggaaat
     3301 catgaactgg gtattttcga tttatttgct gacaaacgct atgcttactc cgatttacgg
     3361 aaaacttgcg gataaagtgg gacggaaacc aatctttatt tttgggacat tattgtttat
     3421 tatcggttcg ttattttgcg gtttatctca aagcatgatt gggttgattg ctgctcgtgc
     3481 gattcaaggg atgggtgctg gggcaatgat gccagtagct ttgacgatca tcggcgatct
     3541 ttattctatt gaaaagcgag caaaagtttt aggtttgaat agttcagcct gggggattgc
     3601 cagtgttttt ggaccgcttg cgggaggatt catcgttgaa acgatcagct ggcactggat
     3661 cttttttatc aatgtgccga tcggaattct attaattatt ttgatttggc tatatttaaa
     3721 tgaaccaaaa atcatacatg agtctaaacc aatggatatt tttggtagtt ttagtttgat
     3781 ggccatgctt gtctcattgc tgttagggtt ccaattatta ggagacgaag gaatgagtcc
     3841 gcttgtttct gctttgctgc taggtagtat cgtaatgtta ggtatttttg tttttgccga
     3901 aaaaagagct aaagatcctg tcatcatgat gcatttattc aaaaatccac tatttgttgt
     3961 ggtcaacttg gctgcagcat tggctagtgg ttttttgatg ggaatcgatg tttatatccc
     4021 tatgtggatg caaggtgtat tagggctgaa tgcagcaatt ggtgggctgg tattagcacc
     4081 attatcacta gtctggatgg tcggttcttt ccttgcaagc aagtggatga ctcaaatgaa
     4141 aactcattgg gtcttgagaa ttggactact aatcgcagct attgggggcg cttggttgta
     4201 tcttatgcct ttcagaagta actatttatg gttttttgct atatcaacgg tcatcggtat
     4261 aggcttaggt gtgaccatga ctacctcgac tgtcagtgca caaaatagtg tatctcgtga
     4321 ccagcttggt gtagcaactt cttttaatac gttagttcga acaattggtc aaacagtcat
     4381 ggtttctatt tttggtttat tgataaatgg ggtaactaaa cgagaacttg cagcagcaaa
     4441 tttaacaaat gatgcagata tcatgaataa actggtgaat ccacaaacgg ctaaatatat
     4501 tgatgcggat attttgttac cattaagaaa aattctttat gatgggttac attccgttta
     4561 tctagttggc ttgttactcg tgatcattgc tttggtctta acgcttttcg ttcatgaaaa
     4621 aagtggtaaa ataaaacagt agtttcggtt aagatatgta cgtagccgaa tcattcaaat
     4681 ttaaagttat tcaaaaataa cttcttacta aggaaggcga aaatatggca tttattcaag
     4741 caaatattta ttcgaatgtg ttagaaatgg aagtcaatct aaatgtgata ctaccgcaaa
     4801 caacaaaaaa ggtgattggc accagttcag agcaagagac aacggatata cctgttttat
     4861 atttacttca tggaatgggt ggcaatcata gtgtatggga aagacgtacg tcgattgaac
     4921 gttatgtagc aaactacgga ttagctgtca tcatgccatc tactgatctt ggctggtaca
     4981 cagatacagt ttatgacatg aagtattgga cattcattgc agaagagcta cctgagattt
     5041 gtcatgaatt attcccgcaa ttaacaagaa aaagagaaaa aacatatgcc gctggattat
     5101 cgatgggcgg atatggcgcg ttaaaactag gccttgcaaa atcagaaaat ttcgcagcag
     5161 taatctctct ttcaggcgct gtttctgtag gaaatcgaat ggatgatctt ttgatggttc
     5221 gtaaagcatc tttttgggaa gggatctttg gaccattgga tcaaatcgaa ggttcaaaaa
     5281 atgatccgca atacttattg aaagaactag taaaaagcgg aaaaccagtt ccaagatttt
     5341 atatggcttg tggggaatct gattttcttt atcaggcaaa tcaggaaatg gtatctgcta
     5401 tgcaggaaca aggcttggcg gtcacctttg aacaaggacc tggcgatcat aactggatct
     5461 tctgggacca atggattcaa cgtgcattgg cgtggctaat ggaagaaaaa taaaaaaaga
     5521 gtcttaaaaa gatagctcct atagagatca caaacatagt gatctctata ggagcttttt
     5581 tactgctcgt tttctcttct tttattttgt tggcataagc cttcaatata ggtttgtgca
     5641 ctcgttagaa agagtgcttt ttcattcaat tctgcatggg actgcatact ttctttaaat
     5701 ccctctaatt gattttcaac tacataatta cgcataaata gaactgtttc tttcaaaaat
     5761 tcatttccgt ccataatttt ttgtcgaagc tgctgttcat gctgctgatc cattaactgt
     5821 ccaatcaata aattgtggag agaggcaatg gcctgatgac tttgggtgac tgaaaatcct
     5881 tcacgaacaa gtaattggat catttgctcc aaatgttcta atcgactcgg aaaagcgggc
     5941 acagtattga tttctaattc tgccccacaa gggaaacgtg tataaaggtc atagtaattt
     6001 tccatatact gcagcaactg ttcattccaa ggcaaagttg gatctggaag cgctaattct
     6061 tcttccattg tttcagccat tatttgcaag agctgttgtt tgtttttgaa ataccaatat
     6121 aaagcaggtg cttgaacatg aattctgttt gcgatgtttc gcatggaaag ctgcgaaaga
     6181 gcttgtgttt cagccaggat atcaaaggcg gcctgaatga tgattgcttg agaaagcttt
     6241 atttccattt tatcaacctc gtgtattgca ttatcttttt aaacatggta aattgttagt
     6301 cggtctaatt ttacagtgtt aaattagtcc tgtcaattta aacaaagggg tgtaatcaac
     6361 gaatgaaatt aatcttacga catgcgaaaa aatataaagt agctgttttt atttccttgc
     6421 tgtccgtagc agtcatggtt gcagcagcat tatggcaacc taagttacta cagcaagtat
     6481 tggaagctat tattacagaa gataatgatg aaatgaaaac aattggtatt tacctgatca
     6541 gtctggcttt gctaggatta gccgcaggtg taacgaacac gattttctct gcaaaagtag
     6601 ctcaaggcgt aagtgcagat atccgagaag aagcatttcg aaagatccag acattttcat
     6661 ttggaaatat cgaacagttc tctgctggaa atcttgttgt tcgtctaaca aatgatatca
     6721 cgcaaatcca aaatttagtg atgatttctt tgcaatcact ttttcgaatt ccattcttat
     6781 ttattggtgc cttcatttta gctatgatca cgatgccgca attatggtgg atcatcgtag
     6841 cgcttatcgt tacggtattt ctgattacgg cactttcatt tacaaggatg ggcaagcact
     6901 tcatgatcat tcaaaaactg attgatcgtg tgaatagcat tgcaaaagaa aacttactag
     6961 ggattcgtgt agtgaaatct tttgtacaag aagaaaacca attgaagtca ttcagcaaag
     7021 taagtgagga cttaacaaaa cacaacatca tagtcggtac attattttct gtgatgatcc
     7081 cgtccttcat gctggctgcc aatttggctg tggttggggc gatctttttt gtgagcgatc
     7141 ttgttaaaga tgatcctacg ttaattggtg gaattgcttc attcatgaac tacttgatgc
     7201 agattatgat ggcaatcatt attggcggaa tgatgatgat gatgacttca agagcagccg
     7261 tatcgctgaa gcggattgcg gaaattttag atgctgaacc agatcttact tatttagacg
     7321 ttccagaaca agagttgacg ggttctgtca cttttgacca tgtttctttt cgttatcctg
     7381 gtgaggatac agatacgctt aaagatatca gcttttctat caaacctgga gaaatggttg
     7441 gtatcgtcgg ggcgactggt gccgggaagt caacactagc acaattgatc cctcgtctat
     7501 ttgatccgac agaaggcaaa gttgaagtag gtggtgtaga tcttcgtcaa gtaaatgaaa
     7561 agagtttaag acaaacggtt tcctttgttt tgcaaaaagc aattttattc tcaggaacga
     7621 ttgctcaaaa cttgcgccaa gggaaaaaga atgcaacaga aaaagagatg gaacatgctt
     7681 cttctattgc tcaagcaaaa gaattcatcg aaaagttatc tgatcgttac gaagcaccaa
     7741 tagaagaacg tagcagtaac ttttctggtg gtcaaaagca acgtttatcc attactcgtg
     7801 gcgttatcgg taatccaaag atcttgatcc tagatgacag tacgagtgca ctggatgctc
     7861 gctctgaaaa gctggtccga gaagcactcg atcgagattt gaagggaaca acgacgattg
     7921 tcatcgcaca aaaaattgct tctgttgtca aagcagaccg catcttagta ttggatgaag
     7981 gcagattagt cggcgaagga acccatgagg aattggtagc tggaaaccgt gtctatcagg
     8041 aaatctttga aactcaaaaa ggaacggagg agtaagtatg actgatctaa taaaagcaag
     8101 taaatttttc tatcactatt tgaagcggta caaactctcc tttttcttta tctttatcac
     8161 agtgatcatt gctacctatc tacaagtaaa ggcacctcaa tacatcgggg aagcttttca
     8221 agaattagct aattacgtag gaggcttgat gcaaggcgta gacgataaaa gcaaatttgt
     8281 ttctgtaata tggaaactct tgctgtttta tgtactagct agtgcagcca actttatcta
     8341 tagtattttg ttcacacaag tagtagggaa gtcaacgaac cgaatgcgta tcggtctatt
     8401 caacaaattg gaaaaattga cgatccgttt ctttgattct catcaagacg gtgaaatttt
     8461 aagtcgtttt accagtgact tagataatat tcaaaatagt ttgaaccaag ctttgcttca
     8521 agtcatcacg aatgctgttt tacttgtcgg gatcttgatc atgatgttcc gccaaaatgt
     8581 agagttagct tgggctacaa ttgcttccac tccagtagcc gttttgatag ctgtcttgat
     8641 tatccgcaaa gcacggaaat atgtggatct tcagcaagat gaagtgggga aactaaatgg
     8701 ttatatggat gaaaaaatca gcggacaacg tgtcatcatc acaaatggct tacaagaaga
     8761 aacgattgaa ggattcttag agcataatca aagtgttcgc gaagcaacct tcaaaggaca
     8821 agcttattct ggtctactat ttccgatgat gcaaggaatg tcactggtca atacagcgat
     8881 agttatcttt ttcggcggct ggttagcatt aaatggtgat ttggaacgtt cggtggcatt
     8941 aggcttagtt gtgacattcg tccagtattc acaacaatat taccaaccac tgatgcagat
     9001 ttcatctgga tacagcatga tccaattagc gattacaggg gcaagacgat tgaatgaaat
     9061 atttgatgaa aaagatgaag tcaaaccaga aaactgtaaa gcatttgatg gtgttgaaaa
     9121 aggtcttgcg ttgcagcacg tagattttgg ttattctcca gaaaaactag tcttgaaaga
     9181 tgtctcgatc aatgtggaca aaggagaaat ggttgctctt gttggtccaa caggatcggg
     9241 gaaaacgacg atcatgaatc tgctaaaccg tttttacgat gtcaatgatg gttcagtagt
     9301 catcgatgga atagatatcc gagattatga tcttgatgca ttacgtagac atgtaggaat
     9361 cgttttgcaa gaatctgtat tgttctccgg aacgatcaaa gacaatatca cattcggcaa
     9421 accggacgct tcagatgaag aagtcatcaa tgcagcaaaa caagcgaata ttcatgactt
     9481 catcatgacg ttagaaaaag gttatgatac agagatatcc gaagaaaata acttattcag
     9541 tacaggtcaa aagcagttga tcagtattgc tcgaacgatc atcacaaatc catctttatt
     9601 gattttggat gaagcaacaa gtaatgtaga cacggtaaca gaagcaagga tccaaaaagc
     9661 aatggatgaa gcaatcaaag gaagaaccag ctttgtgatt gctcaccggc tgaaaacaat
     9721 cttgaatgca gatagaatcg ttgtcttgcg tgatggggaa gtcattgaag aaggcagtca
     9781 tcacgagtta ttagcagaaa atggatttta tgcggaactt tatcataatc aatttgtatt
     9841 tgaaagttaa attaaaagaa cctcgttata acggggttct ttttatatat tttatgttgt
     9901 ttgtttttat aaggggcaga aaaggggcaa tttaaatttc tatttttcct agtttttcta
     9961 ctagttgatt ttgcatattt ttagttgtat gagtataaat ctttaatgtt gtttttgaat
    10021 cttcatgtcc tacacgatcc ataatggctt ttaatgggat tcctaattct gtaagtagag
    10081 aaatgtggga atgccttaac atgtgagaag aaactttttt atctaggttt aattctttgg
    10141 atactgctct taaagattga ttatat
//
LOCUS       DASJTM010000094        10140 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJTM010000094 DASJTM010000000
VERSION     DASJTM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.094"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..188
                     /locus_tag="U4B29_002352"
     CDS             39..188
                     /locus_tag="U4B29_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN2107262.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            199..1713
                     /gene="dltA"
                     /locus_tag="U4B29_002353"
     CDS             199..1713
                     /gene="dltA"
                     /locus_tag="U4B29_002353"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN2107263.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1710..2915
                     /gene="dltB"
                     /locus_tag="U4B29_002354"
     CDS             1710..2915
                     /gene="dltB"
                     /locus_tag="U4B29_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN2107264.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            2960..3193
                     /gene="dltC"
                     /locus_tag="U4B29_002355"
     CDS             2960..3193
                     /gene="dltC"
                     /locus_tag="U4B29_002355"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN2107265.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3196..4464
                     /gene="dltD"
                     /locus_tag="U4B29_002356"
     CDS             3196..4464
                     /gene="dltD"
                     /locus_tag="U4B29_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN2107266.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4605..5687
                     /locus_tag="U4B29_002357"
     CDS             4605..5687
                     /locus_tag="U4B29_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN2107267.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5687..6490
                     /locus_tag="U4B29_002358"
     CDS             5687..6490
                     /locus_tag="U4B29_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2107268.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6560..7300
                     /locus_tag="U4B29_002359"
     CDS             6560..7300
                     /locus_tag="U4B29_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2107269.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7311..8315
                     /locus_tag="U4B29_002360"
     CDS             7311..8315
                     /locus_tag="U4B29_002360"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN2107270.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8360..10057)
                     /locus_tag="U4B29_002361"
     CDS             complement(8360..10057)
                     /locus_tag="U4B29_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107271.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKFYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3251 a         1683 c         2061 g         3145 t
ORIGIN      
        1 gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc
       61 caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc
      121 tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg
      181 aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga
      241 ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg
      301 aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca
      361 attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa
      421 tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc
      481 tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct
      541 tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt
      601 tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact
      661 actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg
      721 acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc gccattttct
      781 tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg
      841 ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag
      901 ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga acctacattc
      961 aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa
     1021 aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt
     1081 ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt actagatagt
     1141 tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa
     1201 ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca
     1261 aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta
     1321 gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa
     1381 ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa
     1441 cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga
     1501 acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat
     1561 tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt
     1621 cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga
     1681 aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag
     1741 agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga
     1801 tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca
     1861 gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga
     1921 cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt
     1981 ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg
     2041 aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg
     2101 tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca tttcgttata
     2161 tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc tataggagat
     2221 ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt ttgggaaaag
     2281 gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat tattttggtc
     2341 aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt tcatgggcat
     2401 tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct ggctatagct
     2461 tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat ttcaataaac
     2521 cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg ctgtcctttt
     2581 ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa aaatttttta
     2641 aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta atgggtatat
     2701 ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt ctgatctgtc
     2761 tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct tctaacaaat
     2821 ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc ttcctgattt
     2881 tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat taatttatat
     2941 aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag aaataacagg
     3001 aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt tgcttgattc
     3061 tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc aagtccctgt
     3121 atctgaattc gagcgagagg attggtgcac accgaataaa atcattcaac aagcaactgc
     3181 attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt tgttttagct
     3241 gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc taatccagaa
     3301 acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa
     3361 aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga attaagcaga
     3421 atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta tcgtccattt
     3481 ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg ttctgcagga
     3541 gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt cgttaaaaat
     3601 ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata cgactggttg
     3661 ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt attaactttc
     3721 tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa aaaagggaag
     3781 ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct gaagagagag
     3841 gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca
     3901 aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa tcaaattgga
     3961 gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac gcatcgtatc
     4021 cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg tttttcgcca
     4081 gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa cgaagtattg
     4141 ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg
     4201 ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata
     4261 gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc
     4321 tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt
     4381 actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata
     4441 ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta
     4501 tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa aaatcataag
     4561 caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat acaaaagaag
     4621 aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa
     4681 gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat gacggtttag
     4741 gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt tctggacata
     4801 tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa
     4861 ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc aaaacacgtg
     4921 aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta acacctcaag
     4981 taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca tcaagccaag
     5041 aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat attgaataca
     5101 aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt atcgggacag
     5161 ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat gttttattcg
     5221 caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg
     5281 taaatcccga tattgctttt gctttagata ctggtacagc aggagacact ccaggaatga
     5341 caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt tttgatgcat
     5401 ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag gaaatggata
     5461 ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa atgcatttga
     5521 caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg cattcccata
     5581 catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca gaattagtga
     5641 aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga ataatagaat
     5701 acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa
     5761 tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact tttttgacgg
     5821 tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc tagtcgataa
     5881 tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag ttgtacgggg
     5941 tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt ttggttcgat
     6001 acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt taatctttct
     6061 gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca gttcaaatgc
     6121 ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt tatcgatgat
     6181 agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat tccaatttgc
     6241 tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga aatatggctg
     6301 gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt ttatcggctg
     6361 gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg tttatgtgaa
     6421 tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc cagacgccta
     6481 ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg taggataaat
     6541 ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag cgtatcctta
     6601 taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt tgttgatcga
     6661 tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg atctagatgc
     6721 tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat tacagtatta
     6781 ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg gacacacaaa
     6841 agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac atccttattt
     6901 tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa caatccatcc
     6961 agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat tcgtatttac
     7021 aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg atttgtttct
     7081 agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca cttcaaaaga
     7141 agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac acctacctca
     7201 gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca aagggattga
     7261 tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac atgatttttg
     7321 taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat ttgctgcaag
     7381 aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca atcatcatcg
     7441 gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa aatggcattc
     7501 atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga aacctgaact
     7561 ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa gtaacacagc
     7621 caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg
     7681 atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc aacggtcgga
     7741 tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc aatgcattaa
     7801 aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga
     7861 atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag
     7921 aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg tacgaattga
     7981 ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga aattgggatt
     8041 ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt tctatcggtt
     8101 cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa atctttggcg
     8161 atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga cagaaatatg
     8221 ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat tttggtgcga
     8281 tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg gaatccacat
     8341 atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt tgaacagtga
     8401 tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt
     8461 ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg atgcctcgac
     8521 taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt
     8581 gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca tgagtttctc
     8641 taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg ttgataggaa
     8701 gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca gctaacttct
     8761 ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac agcatttcga
     8821 aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt tggtccgtat
     8881 gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt gtatttactt
     8941 cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt ttgattttta
     9001 agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt agattgtagc
     9061 tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc gttgtttgat
     9121 agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc aagacgatcc
     9181 aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg catgcaagtg
     9241 aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat tcaggaagtt
     9301 ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt tggaacgtac
     9361 aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag cagatgatga
     9421 aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa
     9481 gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa gcaaagcaac
     9541 ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag atcgtataga
     9601 aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc ggcactgatt
     9661 gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaaatttc ggtggcagtc
     9721 cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg atataatttg
     9781 ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga taatctttcc
     9841 gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt cctttgatta
     9901 gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt
     9961 tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt
    10021 ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc gcaggagaaa
    10081 aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat ataatgatta
//
LOCUS       DASJTM010000095         9957 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJTM010000095 DASJTM010000000
VERSION     DASJTM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.095"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..176)
                     /locus_tag="U4B29_002362"
     CDS             complement(24..176)
                     /locus_tag="U4B29_002362"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2107272.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(367..1245)
                     /locus_tag="U4B29_002363"
     CDS             complement(367..1245)
                     /locus_tag="U4B29_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2107273.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1284..2402)
                     /locus_tag="U4B29_002364"
     CDS             complement(1284..2402)
                     /locus_tag="U4B29_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2107274.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2399..4498)
                     /locus_tag="U4B29_002365"
     CDS             complement(2399..4498)
                     /locus_tag="U4B29_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN2107275.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4549..5370)
                     /locus_tag="U4B29_002366"
     CDS             complement(4549..5370)
                     /locus_tag="U4B29_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2107276.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5367..6176)
                     /locus_tag="U4B29_002367"
     CDS             complement(5367..6176)
                     /locus_tag="U4B29_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107277.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6197..6691)
                     /locus_tag="U4B29_002368"
     CDS             complement(6197..6691)
                     /locus_tag="U4B29_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107278.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6703..7191)
                     /locus_tag="U4B29_002369"
     CDS             complement(6703..7191)
                     /locus_tag="U4B29_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2107279.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7268..9886)
                     /locus_tag="U4B29_002370"
     CDS             complement(7268..9886)
                     /locus_tag="U4B29_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2107280.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEN
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     DYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSP
                     EMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
BASE COUNT         3021 a         2250 c         1648 g         3038 t
ORIGIN      
        1 ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg
       61 aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac
      121 ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta
      181 tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat
      241 gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac
      301 ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt
      361 cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat
      421 tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac
      481 atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt
      541 cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc
      601 acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg
      661 gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc
      721 ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg
      781 ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc
      841 gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac
      901 atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa tattgaaata
      961 ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg ttgatgtgat
     1021 atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc cgaccccgat
     1081 gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac
     1141 tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag taccgactgc
     1201 acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag gttcctccct
     1261 tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt
     1321 ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac atcgtcttgc
     1381 cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga taaatcttcg
     1441 gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg cattgcgtcg
     1501 tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct tgtggcatct
     1561 ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc atgtggacgt
     1621 ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc tgaacctgtt
     1681 gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc gccttcgccc
     1741 caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata gccttgaccc
     1801 tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa
     1861 tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta atttatcgta
     1921 taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt agcggatgtt
     1981 tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac tatcggcaac
     2041 gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa aggactttca
     2101 acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg taaaacaaaa
     2161 ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat tcccgttcca
     2221 gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc acgacctttg
     2281 cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc tgttggattt
     2341 tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt tagtatactc
     2401 atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga atttttttcg
     2461 tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg gattgcgtac
     2521 attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac gttaaattat
     2581 caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat tgactgatca
     2641 ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag tctcgcacga
     2701 cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg tctaaatctg
     2761 ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc agctcgctca
     2821 ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa gtgatgcctt
     2881 tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg gtgttctgat
     2941 ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg tctgattctt
     3001 ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt aaaaagatta
     3061 acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt ttctcctcat
     3121 caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa
     3181 ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc agttgttcta
     3241 gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga ttgctgccgg
     3301 tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa agcattggtt
     3361 cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc agtagaacct
     3421 gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga ctagccagta
     3481 acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat tttgaaccag
     3541 gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac tccggttcaa
     3601 acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga ttagtagcag
     3661 gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg aaactagaaa
     3721 agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc attgacagct
     3781 gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg atatcagatt
     3841 cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg
     3901 ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac acattttgct
     3961 gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga aggctttcgt
     4021 gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta ttagccagta
     4081 tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata tatctaggca
     4141 aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa tggtagacaa
     4201 tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt ttattgaccc
     4261 aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac gtttgaatcg
     4321 tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag gtcaatccct
     4381 ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca tttccgtcgt
     4441 ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta atagtcatct
     4501 tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact atgcgattcc
     4561 aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga tgacgccgac
     4621 ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa ttcctttaaa
     4681 aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg aaaagctcaa
     4741 aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca gacccaagat
     4801 actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt caatatattt
     4861 agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat taggtatatt
     4921 gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta agttgatcgc
     4981 tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa ttccagccaa
     5041 tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac cagctttttc
     5101 agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc aaggaatggt
     5161 cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt cacttttgta
     5221 gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg
     5281 attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca attttttctc
     5341 atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc
     5401 gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt tagttgtaga
     5461 tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc aatcaatggt
     5521 aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat atttttactc
     5581 caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc agcgactgtt
     5641 aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac atctgctcca
     5701 aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa ccacataaag
     5761 aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa aaatttagca
     5821 gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc taatactgct
     5881 agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat tgcgaacgct
     5941 gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat attgacgaca
     6001 cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa atcccccatc
     6061 aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat cccgaacatt
     6121 tcatgtccag caactccaat aaaaacggcc aaagcgacta caaatgcatc atacataatt
     6181 tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt ttttccatcg
     6241 ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt aagtcttgaa
     6301 tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc gatggcggtg
     6361 tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa gctttcgcaa
     6421 cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg
     6481 gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg acattcgatg
     6541 gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca tcgcttgcta
     6601 ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt ccatgaatta
     6661 atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa aatcttcctc
     6721 ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt ggcatttatt
     6781 taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta tttcgagcag
     6841 aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca aaaaggtttt
     6901 ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg
     6961 ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact catccacata
     7021 agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac tgctaagaaa
     7081 gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt taattccttt
     7141 ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca ttggtttaat
     7201 cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag acactttttt
     7261 agacacttta tttttgaata tacggtccta ataattcata gagcagcgaa agctcataag
     7321 cattgacttt aaaattatat ttcatctcca ttgattgaaa aatacttcta gaaacataat
     7381 aaaattcatc ttcatctggt ttcaaatcat aattcggttc ttcagatttt tctgtccgag
     7441 ccaagaataa acgttccatc atcaatgccg tatgcatata gagatttagt tttacacggc
     7501 catttaaagt gaaatgatag taattttcat atttagaaat gactgtttcc acttcactca
     7561 tcacgatcgt tgggttcaga aagttcaaac ggtccgcaat tccttccaac gaaaagaatt
     7621 ttactagctc ctgaatcaag cggttaaatt ctgccggctt catttcagga ctcaaaatat
     7681 gctccaaatt ggctgctcca tcaccgtcta agatgtcata aacattaata ttaggaattg
     7741 aaaaggaacg tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca aaataagttt
     7801 catcattttg gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg
     7861 attcatccga taaatgatga cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg
     7921 acatacagga aatgataatg ttgctacttt gagaaaagcc ttcaaagtat tgaacattga
     7981 ttgaatactt gtctgctgca tcctccgcaa tctgtttaaa tggcgcattc atcgtcatct
     8041 tcaagccaat atcaagtgca atggaagtcg tcaaattatt cagaagaagt aactcccctt
     8101 ttaaatcgtt cttgatctgt gtgtaaattt gatttaaaga gcccatatct acgatcagca
     8161 ctagactatc tgtattcact tgccgtttca cgaaattcct tgtcgattcg acaatctctg
     8221 acacattact ctctactggc atatccaacg cttcaaaaac ataattgccg cataattgat
     8281 tcactactgc ttgaatgctg ctagccgtac tatctccgtg agcaagcaac aggcccttca
     8341 gctcgatgga ttcatctaca taatcagata aagtcacgac aataagcagt cttagaaagt
     8401 tctcgtcctt ttctgagagc ggtggtaaat gctccaagaa ctgattacat aagtaagtcg
     8461 tccgaggtag atttgcagct aatgtacgat ttatcccggt taagtctgtc ttatagcttg
     8521 gggaaatgac ataggatctg ctaataattt ctgcaacagg tccactcgtc gtcaatccgt
     8581 acgtttttcc taaaacggat acccagacct ttctaaacat ctgaatcatc aattttctac
     8641 tcattgataa aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact tcattcttta
     8701 tcgaagaaaa attttcattg aaattacctt catttatcag ctgagtaatt ttttccaaaa
     8761 tcgaatcata atcaaacggt ttgattcgat ccgtttttat cttttgattt ttctttatca
     8821 ataaactagc aatcggcaag ctttctactt cggattccag catatttaac tgaatcaaga
     8881 tctcattttt cccaatcgtt aatggttctt cttgtgttcg actatacaca tccgcacaac
     8941 tgattttaat gatattcttc agcttaccaa tattcccact aaagggatga gataacatta
     9001 aagacaaggc ttctcttgaa acaataatat ctttttgtaa aacatccgct tcattttgaa
     9061 aaaataattg aatcagctct aatcgttctc gaatggggcg atcttcaaaa gttggcaaca
     9121 taacactaat ttgaatacgc cgatcaaagg tgtctaataa ataattttca cctttttccg
     9181 tcgtggcgaa aataaaacga accttagcag aatgccaatt tacattttca ccgactggac
     9241 gatagtttcc agtatcaata aatagaaaga gcttttcttg attttctttc gataggcgat
     9301 ggacctcatc gagaaaaaga tagccgttgt tggccgttgc caataaacca tcggtatcct
     9361 tttcagcccc cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa agcagttcgg
     9421 gattgtccgc ataatcggca cagtttaaga tagcaaatgg tgcatctttt tcaataattt
     9481 gctcatggat agcgtattca aaaatttttg tggctaaaaa actttttcca acacccgtgg
     9541 ctccatttat taatacactg agtccgttag gcggatattt gacagcggct atacattttt
     9601 gaatgacctc tctcagactt ccgtcatgac caactacaga atgaaaggct tcgtttattt
     9661 gctccgtatt atctacaacc gcatattcat ctaaaggctt ccacaaaaca ggctttcccg
     9721 gtaatttctc gaccttttta tcttccacta atctcgtcaa ataatggctg gcattctgac
     9781 gagagatacc tagattcttc gaaattttct cggcagtgac agaagacata ttccctgtta
     9841 gtcgtatcag ctcatcaaaa actcgatctt tagcgctctt tttcattttt tcctccagtt
     9901 taatgtgtct aaaagcataa taaactatcg gctgcaatat gtctattcta tatttgt
//
LOCUS       DASJTM010000096         9811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJTM010000096 DASJTM010000000
VERSION     DASJTM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.096"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..2154)
                     /locus_tag="U4B29_002371"
     CDS             complement(34..2154)
                     /locus_tag="U4B29_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2107281.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="U4B29_002372"
     CDS             2384..3562
                     /locus_tag="U4B29_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN2107282.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="U4B29_002373"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="U4B29_002373"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN2107283.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="U4B29_002374"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="U4B29_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN2107284.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="U4B29_002375"
     CDS             complement(4915..6168)
                     /locus_tag="U4B29_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2107285.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="U4B29_002376"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="U4B29_002376"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN2107286.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="U4B29_002377"
     CDS             7746..9092
                     /locus_tag="U4B29_002377"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN2107287.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="U4B29_002378"
     CDS             complement(9400..9786)
                     /locus_tag="U4B29_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2107288.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DASJTM010000097         9790 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJTM010000097 DASJTM010000000
VERSION     DASJTM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.097"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..311
                     /locus_tag="U4B29_002379"
     CDS             <1..311
                     /locus_tag="U4B29_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HEN2107289.1"
                     /translation="AEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRL
                     QNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD
                     "
     gene            346..1113
                     /locus_tag="U4B29_002380"
     CDS             346..1113
                     /locus_tag="U4B29_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2107290.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1305..1703
                     /locus_tag="U4B29_002381"
     CDS             1305..1703
                     /locus_tag="U4B29_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN2107291.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2010..2384)
                     /locus_tag="U4B29_002382"
     CDS             complement(2010..2384)
                     /locus_tag="U4B29_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107292.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2406..2783)
                     /locus_tag="U4B29_002383"
     CDS             complement(2406..2783)
                     /locus_tag="U4B29_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107293.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(2773..3168)
                     /locus_tag="U4B29_002384"
     CDS             complement(2773..3168)
                     /locus_tag="U4B29_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN2107294.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3235..3420)
                     /locus_tag="U4B29_002385"
     CDS             complement(3235..3420)
                     /locus_tag="U4B29_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEN2107295.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3502..3756)
                     /locus_tag="U4B29_002386"
     CDS             complement(3502..3756)
                     /locus_tag="U4B29_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107296.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(3778..4023)
                     /locus_tag="U4B29_002387"
     CDS             complement(3778..4023)
                     /locus_tag="U4B29_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107297.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4030..4314)
                     /locus_tag="U4B29_002388"
     CDS             complement(4030..4314)
                     /locus_tag="U4B29_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107298.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4417..4599)
                     /locus_tag="U4B29_002389"
     CDS             complement(4417..4599)
                     /locus_tag="U4B29_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2107299.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(4805..5344)
                     /locus_tag="U4B29_002390"
     CDS             complement(4805..5344)
                     /locus_tag="U4B29_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107300.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(5457..5723)
                     /locus_tag="U4B29_002391"
     CDS             complement(5457..5723)
                     /locus_tag="U4B29_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2107301.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5725..5946)
                     /locus_tag="U4B29_002392"
     CDS             complement(5725..5946)
                     /locus_tag="U4B29_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEN2107302.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6172..6990
                     /locus_tag="U4B29_002393"
     CDS             6172..6990
                     /locus_tag="U4B29_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2107303.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            6994..7290
                     /locus_tag="U4B29_002394"
     CDS             6994..7290
                     /locus_tag="U4B29_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107304.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7472..7825)
                     /locus_tag="U4B29_002395"
     CDS             complement(7472..7825)
                     /locus_tag="U4B29_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107305.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(7845..8900)
                     /locus_tag="U4B29_002396"
     CDS             complement(7845..8900)
                     /locus_tag="U4B29_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HEN2107306.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9116..9514
                     /locus_tag="U4B29_002397"
     CDS             9116..9514
                     /locus_tag="U4B29_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107307.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9517..9690
                     /locus_tag="U4B29_002398"
     CDS             9517..9690
                     /locus_tag="U4B29_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107308.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3190 a         1538 c         1707 g         3355 t
ORIGIN      
        1 gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat
       61 acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc gatcttaaac
      121 ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag
      181 gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag gattacacaa
      241 taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct tatgtgaacc
      301 tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa aaaagatgaa
      361 ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga tttaaagtcg
      421 tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa gaaaacaggc
      481 gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca aaatcttgaa
      541 acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga aatttcgaat
      601 ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat tcattgggat
      661 ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt aattgtctca
      721 atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca aaaaaaatat
      781 agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat cgccaaaaat
      841 aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat agttaaaatt
      901 cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga tagacgtgtt
      961 ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga ttttgctaaa
     1021 gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag tgagtttgat
     1081 gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac tcgtttgaag
     1141 atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta atctccttta
     1201 gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct tttttttgaa
     1261 acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa tacttttttt
     1321 gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat tttccagact
     1381 tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa gtattggaag
     1441 gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag
     1501 agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta aaagctaata
     1561 cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg actgtggaac
     1621 actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct ttgaatgatt
     1681 tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa aaacagcttg
     1741 ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa aagctctcag
     1801 aagccttctg agagctttta aattgctatg tattgtactg catctgctca aattgatttt
     1861 cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata tgtattgagt
     1921 aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat gtgttaggat
     1981 aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt tttatcggta
     2041 ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat ccctgcatcg
     2101 ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat caaaatggtg
     2161 tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga cttataaaaa
     2221 agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta tttccaaaga
     2281 tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc tgctaattga
     2341 tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct cttctcctgt
     2401 ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc ggtttgaaat
     2461 tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga ttcacttaat
     2521 gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt atagcagaat
     2581 tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa gttattatcc
     2641 tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct
     2701 tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc tttcataggt
     2761 tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa gtagttgaga
     2821 gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac taacattttt
     2881 tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt ggatgattac
     2941 taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag gaaattctgc
     3001 atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat atgcttcttc
     3061 aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt tataagcttc
     3121 tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt cctcgctcct
     3181 tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag gctgttaaag
     3241 ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat tgtggatcgg
     3301 aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc cagtttgtcc
     3361 agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg tcttaggcat
     3421 agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat caatagaaaa
     3481 agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag caaattgaat
     3541 gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat aggtatctaa
     3601 gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt tctccaagtc
     3661 ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta tgaaaacata
     3721 ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt tcttgtacta
     3781 agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata ggacgtgacc
     3841 gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa ggctagtacc
     3901 tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg gatcaatgcg
     3961 agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt ccaaagtcca
     4021 cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt caatagtcac
     4081 ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca agttcccgat
     4141 gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca cttgccgact
     4201 gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg cattaattgt
     4261 ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta gcatcttgtc
     4321 actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc ggtgtgtata
     4381 atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa acgataaagt
     4441 aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg ctttgcttgt
     4501 gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc cagtaaagaa
     4561 taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc cttctgtaat
     4621 attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat ggtgatgttt
     4681 ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt acctaatacg
     4741 tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt tatgtattta
     4801 agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga gtgagtgatc
     4861 catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca gcgacatacg
     4921 atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt tttggtttat
     4981 tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga tcaccgacaa
     5041 agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca tttaagaagt
     5101 agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag gttccttctg
     5161 catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg atttccaaac
     5221 catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa taatcaccat
     5281 accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca cttatattat
     5341 acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa gtgggatcgt
     5401 aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct tgtctgttac
     5461 ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc atgctcgcta
     5521 atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga tttattagct
     5581 gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt ctcgatccag
     5641 tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg agcttcacgt
     5701 tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat cttcgtggga
     5761 aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg caaagtctac
     5821 taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt tatctccgtt
     5881 gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa tacttattac
     5941 tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg acaaatgcaa
     6001 tagttatata tttgaatata tacataaaca taaacatata catatatatg tttatgttgt
     6061 agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata tacataaacg
     6121 tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat catggaaaag
     6181 aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac aacaacaatc
     6241 tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa
     6301 gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc gaaaagagag
     6361 aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat aaatgagaac
     6421 atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat gcgtgatatg
     6481 cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca gcttaaagag
     6541 aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc tcaaaatgct
     6601 tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg ttttgaagct
     6661 ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga tattaagact
     6721 ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa acataatact
     6781 aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct tggatatgta
     6841 caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc tgagttcgct
     6901 aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt tacgctatac
     6961 aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga aaatagattt
     7021 aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc agttgaagaa
     7081 cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa aagtgatagt
     7141 tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa gaaattatct
     7201 aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga aatgatagat
     7261 aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact ttttttattg
     7321 gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg atatatacgt
     7381 atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac ataataaaaa
     7441 ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta cttggtgcat
     7501 tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta accgttgaag
     7561 gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct gctactttgc
     7621 tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt acacgccgat
     7681 ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt tcatattcgg
     7741 attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt ataactacat
     7801 aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg agttaaacca
     7861 aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa ggtatgcgga
     7921 attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt aaagacaact
     7981 ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac catattggtg
     8041 tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt tccgcttggc
     8101 ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag ttggaaagca
     8161 ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt aaaaatgccg
     8221 gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac atcaaaaagt
     8281 ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt ttcatcttgt
     8341 acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg tggcagtacc
     8401 caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga atgttgtcgt
     8461 gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga aggcagacct
     8521 aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc taggttagct
     8581 aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat cccaaaacct
     8641 agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc aaaataatag
     8701 ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt taatgaggaa
     8761 cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg agagggaagt
     8821 gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa gtcaacggaa
     8881 aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg tatttcggta
     8941 cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa aaaatgcaat
     9001 cataaaagtc taattaatta taataaagtg taaataaaaa ggtgtaaata aatgcttgca
     9061 aatttatctt attatgatat tataaaatca taaattatat taaggagtga tcctgatgcg
     9121 attgagaaga aataagaatt acactaccat tattttagaa gattttactg atcaagaatt
     9181 acttattgtc ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt caatagtaaa
     9241 agcttcaaag gttgctgtcg aaaaatattt tgattcttta gaagaaagtg ctaaggaaat
     9301 ttttaatgga gagaaagctg gtagctatga gttagacggt attagattta gcttgatccc
     9361 aacaactgaa aatattattc cgaagaaatt atattatatc ttttcatctg aaaaacatac
     9421 ttcaatgaag ccttgtattt ttgattggca atggttgatt gatgctgtat tggatgtaaa
     9481 atgttcaaaa tacaatatta ctttggggtg ttaattatga gtatgctagt tagaaaatta
     9541 ccattatctg taagagaatt tctattttac ttggtctgct taatcgcatc agggtttaag
     9601 ctgatcgaac atatttttat ctttgcttat gtattagcaa tgatcgcatt tttatctggc
     9661 ggttggttaa ttatttattt gttaagctaa gttttagatc aaaaataatt agtaaattaa
     9721 acgataaaat aaccgttaat aaatgtaatg aaaggacaac gcctaacaat cagcttgtaa
     9781 taagattata
//
LOCUS       DASJTM010000098         9758 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJTM010000098 DASJTM010000000
VERSION     DASJTM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.098"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            377..1684
                     /locus_tag="U4B29_002399"
     CDS             377..1684
                     /locus_tag="U4B29_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2107309.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1803..3002
                     /locus_tag="U4B29_002400"
     CDS             1803..3002
                     /locus_tag="U4B29_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2107310.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(3029..4297)
                     /locus_tag="U4B29_002401"
     CDS             complement(3029..4297)
                     /locus_tag="U4B29_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN2107311.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(4460..5106)
                     /locus_tag="U4B29_002402"
                     /pseudo
     CDS             complement(4460..5106)
                     /locus_tag="U4B29_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(5514..6017)
                     /locus_tag="U4B29_002403"
     CDS             complement(5514..6017)
                     /locus_tag="U4B29_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2107312.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(6140..6904)
                     /locus_tag="U4B29_002404"
     CDS             complement(6140..6904)
                     /locus_tag="U4B29_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN2107313.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            7012..7287
                     /locus_tag="U4B29_002405"
     CDS             7012..7287
                     /locus_tag="U4B29_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN2107314.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            7725..8675
                     /gene="yidC"
                     /locus_tag="U4B29_002406"
     CDS             7725..8675
                     /gene="yidC"
                     /locus_tag="U4B29_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2107315.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(8854..9054)
                     /locus_tag="U4B29_002407"
     CDS             complement(8854..9054)
                     /locus_tag="U4B29_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107316.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         2943 a         2039 c         1748 g         3028 t
ORIGIN      
        1 aaaacggttt ctacggtttt aaatttagct atttttttaa gttatcttca taatttcctc
       61 aattatttag ccccagtctt caactattct tcatgaaatg ctgataaact aagaaaaaga
      121 ataaaagagg tggatcttat gcatttgatt atgattttca gtttattaag tattatcagt
      181 ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa cacagcctat tcctgtaaaa
      241 gtacgtaaaa acgaccgcta aacttccata acgcgcgttc ttatcctaaa gttttttcag
      301 aaacattgaa actctctccc ctctctgata aaattttaat ttgtagcgaa acaaattaat
      361 tacaaggaga tcggatatgt ccataaaaaa aacacgtcta gtcctattat tggctagtct
      421 cattctttgc cttgcttctt ttctaccagc tgttgctgtg caagctgaag atacgttcaa
      481 agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc ggtaagattc tttatgatca
      541 agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa atcatcggtc tttacatcgt
      601 ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat aaagtttcga tttcagatta
      661 tgcagaaaat ttgagtatca ccccagattt atcgaatgtt cctttgcata aagaaaacac
      721 ctacactgtc aaagaattat tcgactctgc aatcatccag tcagccaatg cttcaatggt
      781 ggccttggca gaaaaaatct ctggcagcga agctaaattc actgaacgaa tgaaagaaca
      841 attgaaagat tggggaatca aagatgcaac aatcgttaat gcatcaggat tgaacaactc
      901 ttatcttggc gaaaaccgac cagaaggcac tggagaaaac gacgaaaatc aaatgtctgc
      961 tcaagatgtg gccatcgttg cccgccattt gatcttggat ttcccagaaa tactggatgt
     1021 atcaagtact acgacccaaa tgtttggcga aaatacgcaa tcaccagtgg aaatggtcaa
     1081 ctggaactgg atgctcccag gattcgttaa ttacaaggaa ggcgtggacg gattaaaaac
     1141 tggtacgact gagcttgcag gtgcttgttt cgttggtacg atcactaaaa acgatcaacg
     1201 gatcatcact gtcgtattga atgcggacgg ccatgcggaa aacccgagtg cccgcttcaa
     1261 tgaaacagac cgtttgatgg actactgcta tgacaattgg tcagaaaaag agctcggaaa
     1321 agctaacgca agtattccta gtctaaaaac cattgatgta aaagacggaa aagaatcaac
     1381 agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta cgaaacgata tggatactgg
     1441 caaattaacg atcacgccaa cacttgacaa gaagcaagtc actgacaatg agatccaagc
     1501 acctatggat aaaggtacaa aaatcggtac cgctacgatc accttggcag acgataaatt
     1561 aggctattta gaagacaaca atgcaccatc aacagagatc attacggata aaacagtgga
     1621 aaaagcgaat atattcgtga ttggctggcg acgcgtcgca gaatttttcg gcaacttgtt
     1681 ttaattgaac aataagaaaa acatctttcc atcggaaaga tgtttttttg ctttttctag
     1741 tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat
     1801 ttatgaatct aaccagagat ctgatctttt tagttctcta tcttattggg gtattctcta
     1861 tcgtttattg gcgaaaacag aagaaggatc cttccttgtt cttttacggc agtacgtgga
     1921 catttacggt aatcttaact tattttgtcg tgttggaaat tttttattcg accttttatt
     1981 ttttgattcc attgttgttc tttagtatct ttgctttttc ttatttcaca gaaaaacgaa
     2041 ggctactgaa tggtctttta ttcaatgttt tactcatcag tttcggcatc tacttgtttg
     2101 tcttgctgta tgaaacgcaa aatatttttc ttggcggact gatcgccttg atcacgattc
     2161 cattgctact tgtcctttta tttggtattt atggattgat tgtttttctt ttttggaacg
     2221 gggtcacagt tttacgtaga gaatcgcatt cattagctaa tcttttaacg ttgatactag
     2281 ccatttttct tacactgttt cttgtttttg actttttctt gttaaaatat ttgccgcaat
     2341 ggataaatgc tttgtttttc tgcgttcccc tgatcttgat ctatctattt atcgtattct
     2401 acaacttttt gactgtctca ttcttgtatc agttcaaccg gcctcgatac aatcaagact
     2461 tcatcgttgt gttaggcgct ggattgatca atggagaaac agtttctcct cttctagcca
     2521 agcgaatcaa taaggcaatt gctttttatc gggctcaaag ccgtgctaca ttaaatccgc
     2581 ccatcttatt gatgtctggc gggcaaggag cagacgaaaa agtaccagag gccattgcga
     2641 tgaaacaata tgccatggaa caaggaatcc ctgaaagaga cattcttgtc gaaaccaatt
     2701 cgacaaccac tttggaaaac atgctctatt caaaggaaat catggatcag caaatgaaag
     2761 ggccataccg cgcgatcttt agttccaaca attaccacat ttttagagcc ggtctttatg
     2821 cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa aacagctttc tactacttgc
     2881 ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat gaacaaaaaa agacatatga
     2941 tcatttgcgg attgcttttc gcaggctccg cattcttatc gctgattact ttacttttct
     3001 aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttc attctcttaa
     3061 ctattggcaa aaagagtatc ccttttttga ccgctacttg ctgagcagaa taaataccgc
     3121 tgttgcctga acacataaag ctgatcaagc agatcataaa gaagtatact gccgcttgtg
     3181 aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc gcaccagaaa
     3241 atactccgat aaagcccaat cctgcaagaa aaggaacaga aacatggagc aaaggcgcta
     3301 gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt cacttctccc ccttgatatc
     3361 ccgcgcctaa agacaaaact gtgaagaata acttcccaat aaaatcatag acatgtgcct
     3421 gtccattaaa tgattcttga agtaacggaa gactcaatcc gagataacgt tgtgtttgca
     3481 aaatcaatgc tgctgccaca actacagctc cacctatcgt atttcgacaa acaggatgag
     3541 gaatccattg tgcatatcgc gcttttaaaa agacgatcga acggctgaat acccaaccaa
     3601 tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag
     3661 gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc atgaaatttg
     3721 caaacagtgc agcaaagaag ctagggaaga tcgcttctgt tcggactttc ccgattgcca
     3781 acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg
     3841 cactgattcc actgataatc aagatttccc gttctgattt atccaatcgg aacaaacgtg
     3901 ccgtattatc tgctaacgct ccccccattt ggactgctgt tccttcacgg ccaacagatc
     3961 ccccaaataa atgggtagtt atggttccaa acagtgtaag tggaataagc cgcaacggga
     4021 ttttttcttc tccaccattt ccttgatcga tcactaagtt gtttccacga ctagaaagtc
     4081 cgccgtgata cgcatagaga taagcaaaaa gcgcaccact aactggtagt agacaaagga
     4141 gccaaggatg tgatagtcgg atatctgtca caactgtcag gctatttaag aaaaaagcag
     4201 acaagctccc catgaatata ccgagaatcg ctgtgatcac cagccacttc agcatataca
     4261 ctaaaatgcg tactggactc ggtaattgtt tttccataaa aaattcctcc tcaataaaaa
     4321 aacgcctact aaagaaaggg gctgtcccca gactttagta ggcgtcatta gctatctttc
     4381 gttagcattt aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt
     4441 tctgtaaaaa acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag
     4501 gagccaattt cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct
     4561 ttttcgtata cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gaaacattcg
     4621 atgttttctg acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa
     4681 tcccttttaa tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat
     4741 tccagccgat gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt
     4801 ttttcacttt tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgaa
     4861 cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta
     4921 aaactgtcgt taaaaatgta tcgattctgg cagttcgtga tctaatatgt ctcgaatctt
     4981 ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc
     5041 atttaataac tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg
     5101 ttccatgatt ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat
     5161 cttctgcaaa gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc
     5221 atcttatcat cacccccttt aatgcttatt attctatgta catgattcat tcgataaata
     5281 aaagtaaacc gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa
     5341 agtcgtgtca aagtcccttg tttcgaatga atggtgttca aaagctggca tctgcggtaa
     5401 taagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
     5461 aagctgacca cttctgtcac aacctcaata tggtctatta ttggttttat ctattaaatc
     5521 gaggattctt tcccaaagta acgttgccat ctcttacgca tacttgctga cataggctga
     5581 gcggcttctt tgactgcgca gtatttgtct acgaatgtta cgatatatcc ttctttgtac
     5641 ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa tgatatctcg ctcaagatcc
     5701 gacaattcgg tgatcttttc tgcattcttt acagcaattc ttggatggat atacgcatgt
     5761 gtgccttcat caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca
     5821 gcacgcgctg ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa
     5881 gaaacactga tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat
     5941 tttttcactg cttctgtttc taaaagatct tctacataag acatatattc ttcgtcttca
     6001 cgccattttt ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa
     6061 attgatctaa gtataccacc agtgttcgga ttattctagt cgccttggat aaaatagcca
     6121 aagcttaata gttccttaat cataaagaaa aatgataaag aacaatccct gccgcaaccc
     6181 caacattcaa cgattccgca tttcctttca tcgggatata tacgttctga tcagtcaaag
     6241 acaagatttc ctgacttacc ccctgacctt cgttccccat gattattgcg aagttatctg
     6301 atttttctac tttgtctaat gcgtgtgctt cttcatttaa ttctgtgcca tacactgtaa
     6361 accctttgtc tttcattaaa gaaatcactt ctgtcaaatc tttttgcaac acaggcaaat
     6421 gataattgct tccctgcatg ctgcgcagca cctttgtact ataaatatct gccgttcctt
     6481 tgcctaataa aacaccagcc atatcaaatg catctgccgt acggatcatc gtccctacat
     6541 ttccaggatc ttgtacatta tctaacaaca gccatcctcc tgaaaaatct ggcgtgcctt
     6601 cagccgttaa cgccatcact gctaagattc cttgtggtgt aggtagatcc gatacagcat
     6661 ccatgacctc atccgaaact agaaaagtag aaatctcttt gtttgcggcc agccagtctc
     6721 cccattcttt catgcctcgc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg
     6781 cagcttcttc aatcagatgg aacccttcca aaagatattc ttccttttct tcccgatatt
     6841 tctttttatg tagttttttt aattctttga ttcttggatt ttttgctgat tggatctctt
     6901 tcacattcct cacctcccca tgattatatc atggaatagg tgcttgagct tgtttcatcc
     6961 taaaaaaatc ggtatcatta atttataaaa taggaaagaa aggtgacgaa catgcgcaaa
     7021 gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat ttcgctacat gaccaagatg
     7081 gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg aagatgacgg ctcagtcacg
     7141 atcgaagcaa tcggcaatga cgacatcatc cagaaattta ttgaagaagt caagaaatct
     7201 ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag aagacccgat gattgaggaa
     7261 cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag ctggtctgca tcaagcatta
     7321 aggacaggtt gtgaaaaata gctctccata tttttcaaca ggctggactt aatgccgtag
     7381 atgcaagctt ttgaacaccg tcgataaggt tattcttgag gcgctttgcc tcaaaaatta
     7441 aggacagcag tgaaaaagct gatctatcac ttgcagtgag ttcatttgac tccgcttttt
     7501 cgggcagaaa aaaagttttt agtttattaa agtaagaaag aaacacttgg aatcattcta
     7561 ctttttccta gtatttcttt cttgattttt tgtctttttt tcaaaatttt tcgctacgta
     7621 aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta
     7681 gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa
     7741 actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc tgtgtgcgga
     7801 tggattcaaa cggaaaccct gacacttcgg gaatcgttta tcgagtactc gtccatccta
     7861 tgggccaagc gattacatat ctagttgaaa atttcaattg gtcttatggc tgggcagtta
     7921 ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact aggtattagt caatcgaaaa
     7981 aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcccaac
     8041 aaaaattgaa agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc
     8101 aagtataccg cgaaaacgga ttgagtatga ccggcggaat cggctgtctg cctctattga
     8161 tccaaatgcc gatcttctct gccttgtact ttacggcacg ttatacagaa ggtatccggg
     8221 aatcatcttt ctacggcatc gatttaggct caccaagtat ggtacttgtt gccattgcag
     8281 gggtcgctta tctgcttcaa ggatacatct caacgatcgg tattcctgaa gaacagaaaa
     8341 agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg
     8401 cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat
     8461 tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac
     8521 aaaacccacc aaaacaagtc gtcactccaa gaattgatgt gacagaatca gctaaaccag
     8581 ctgacagcaa acaagcaacc aaacaaatca agcaacccaa aaacagtaca aacaataaag
     8641 gccgcaacgc aggaaagcag cagaagcgga agtagatagt aaataagagt gtaataaaaa
     8701 ttcattaaca gttttagcaa ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt
     8761 cgatattttt atttaagtga atgtttaatt tgaatcatcg attgatcgct atctaattgc
     8821 agcagcatct ttttatgaag aaacaacttt ttcttatcta tcaacgtaga caggtgttgc
     8881 tgtttcttct aatagtgtac ttaattcctt aatattttgt tttccttctt ctaacatcca
     8941 actactcgct ttttcttcgc cttcggtaac aaaaggcgcc actcccttcg cccatgttgc
     9001 tcgcccacat aagacaccat tctccataaa ggacaacgcg gagataccac ccatctaaat
     9061 aacatccata aacctttcta gtagaagaat agtgatagta tccatccctt tttctactgg
     9121 gaatacaatt acacgattca aagcttgata cacaacattt cccttcttat aaaccgctat
     9181 tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa ctggctcctt tccgcaactt
     9241 cttaaatgtt ggtttctttt atactttctt tgtagcaact acaaatttct tcaaatctct
     9301 aaatgtttgc ataacaaccg cctttctgac ttttactcag gatggtattt aaaaatcagc
     9361 aaaataaggc ttctctccat cttttttatt tcctagctgc tttaaagaca ctcttgctgt
     9421 tttttaatct ctatcaaggg ggctctgaat ctattttgat gaacttatct tattaatttc
     9481 tcaaatgagc tgtcagacac aactgtattt tttcaatctc ataccggaca actatgccag
     9541 cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa atgacaatga aaaaagacca
     9601 ttctttttgt tgaatgctca ttgtgtaaac atatttcctg cgactgctct tcgaaccatt
     9661 ctcagtgcaa tccctaattt atctattaaa tcttctttta gttcctcatc tttcatattc
     9721 aaaatttcct ttgtatgtta cacaagtttg ttctctaa
//
LOCUS       DASJTM010000099         9681 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJTM010000099 DASJTM010000000
VERSION     DASJTM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.099"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="U4B29_002408"
     CDS             51..260
                     /locus_tag="U4B29_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107317.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="U4B29_002409"
                     /pseudo
     CDS             467..667
                     /locus_tag="U4B29_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="U4B29_002410"
     CDS             complement(1379..1984)
                     /locus_tag="U4B29_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107318.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="U4B29_002411"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="U4B29_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="U4B29_002412"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="U4B29_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="U4B29_002413"
     CDS             3019..3990
                     /locus_tag="U4B29_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2107319.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="U4B29_002414"
     CDS             4130..5443
                     /locus_tag="U4B29_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2107320.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="U4B29_002415"
     CDS             5486..6352
                     /locus_tag="U4B29_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2107321.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="U4B29_002416"
     CDS             6349..7197
                     /locus_tag="U4B29_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2107322.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="U4B29_002417"
     CDS             7211..8683
                     /locus_tag="U4B29_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN2107323.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="U4B29_002418"
     CDS             8700..9572
                     /locus_tag="U4B29_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2107324.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3037 a         1659 c         2019 g         2966 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa g
//
LOCUS       DASJTM010000100         9593 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJTM010000100 DASJTM010000000
VERSION     DASJTM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.100"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..641)
                     /locus_tag="U4B29_002419"
     CDS             complement(<1..641)
                     /locus_tag="U4B29_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN2107325.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="U4B29_002420"
     CDS             complement(643..1407)
                     /locus_tag="U4B29_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN2107326.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="U4B29_002421"
     CDS             complement(1415..2092)
                     /locus_tag="U4B29_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN2107327.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="U4B29_002422"
     CDS             complement(2129..3514)
                     /locus_tag="U4B29_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2107328.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="U4B29_002423"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4B29_002423"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U4B29_002424"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U4B29_002424"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN2107329.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="U4B29_002425"
     CDS             4453..5310
                     /locus_tag="U4B29_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107330.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="U4B29_002426"
     CDS             complement(5391..5726)
                     /locus_tag="U4B29_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107331.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="U4B29_002427"
     CDS             complement(5793..7163)
                     /locus_tag="U4B29_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN2107332.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="U4B29_002428"
     CDS             complement(7241..7777)
                     /locus_tag="U4B29_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2107333.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="U4B29_002429"
     CDS             8086..9567
                     /locus_tag="U4B29_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107334.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DASJTM010000101         9535 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJTM010000101 DASJTM010000000
VERSION     DASJTM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.101"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..865
                     /locus_tag="U4B29_002430"
     CDS             128..865
                     /locus_tag="U4B29_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107335.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(882..1298)
                     /locus_tag="U4B29_002431"
     CDS             complement(882..1298)
                     /locus_tag="U4B29_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107336.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            1691..2938
                     /locus_tag="U4B29_002432"
     CDS             1691..2938
                     /locus_tag="U4B29_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2107337.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3206..5596
                     /locus_tag="U4B29_002433"
     CDS             3206..5596
                     /locus_tag="U4B29_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HEN2107338.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            5645..7480
                     /locus_tag="U4B29_002434"
     CDS             5645..7480
                     /locus_tag="U4B29_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2107339.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            7549..8412
                     /locus_tag="U4B29_002435"
     CDS             7549..8412
                     /locus_tag="U4B29_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2107340.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            8402..9511
                     /locus_tag="U4B29_002436"
     CDS             8402..9511
                     /locus_tag="U4B29_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2107341.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         3522 a         1462 c         1904 g         2647 t
ORIGIN      
        1 agatgataat tgtaatatag aattgtagac ttaaataaag cataaaatac cttttatgat
       61 aaggtatagc tcttaatttt aagcagtttt agatataaga ttgaaagtaa agtgggggag
      121 aataataatg gcagatcata aattagaaaa gttattagaa gaaacgtgga atccaaaaga
      181 atttagtgaa ttttttatgg agaattttga aactgactta gcagtgattg ttaaagatgc
      241 tcttagagag caagggtatc ctgaaactgc aaactatata aacattaatt ttactttgta
      301 tactgaaaat aaaggaactt gggatttttg ggctacttta gcaaataagg agttaagtga
      361 taaatcagac acaggaattc gtaacttttt tgaatcaaac agagatgatt atatgtatgc
      421 taacaatcaa aataaattga actttagagt agagtttgat gaaacacctg aagaatttat
      481 agaaagacaa ccgcctaaag aaaatgtagc aaaggtattg gaagatcgtt ggaattcaga
      541 tgaaatagtt tcaacaatta gcgaactagg cggacaatat gaaccgctag tagaagcggt
      601 tagagaagag ctaagactga ataagtttcc tgatgttcaa aatattgatg tttctcaaat
      661 tgaaataaac gtcaagatca cgaataaact tgattatggt agttgggcag atatagcatt
      721 agaaaaatac atctactcaa cgttaaaaga gtttatcgaa aatagaatgg atatcatgta
      781 tctacagcat ccacagtatt taaactttgg tgttgaaata gctacacctc ttgaagaatg
      841 gaaaatggag caagggcttg actagccttt gctccatttt tttattttac aaatgaatac
      901 agttcgtcgt ttttaccaag aatgattgtt tgcccaactt tgagattgtc aggtgatcct
      961 tcagcggttt gtaaggagaa cgttccattg aacttatcgg ataccgttat aggtagcttt
     1021 aattctgatc cttcctcatc tttgagaatt gctgtttttc gttcatcaaa ataaacagtc
     1081 tttattaaat aatttttcgt agtgatctca gcagatttgt ctttttttgt agtagtacca
     1141 gtttctttag aattagaggt attttctacg actattccat ctttggcatt tgagcttgtg
     1201 tttatttgat tgaatatttt cagcgacaag ataacgctaa gaatagtcaa tatagaaaca
     1261 agtgtaatta atactgataa tttccttttt ttgttcattt tttcttaaca tccttttttg
     1321 ttttgattag ttagatattc cttatctaac aagccttttt aggctattaa atgaagataa
     1381 accccaaaaa aatattgcaa ttatgacagt gcaatggtac tattattata caataaattt
     1441 tgcgaaaggg gtaggtctaa atcgcaaaca aaaagaaatt gaacctagac aaatacaact
     1501 tgtataccgc aagggaagca agtttgattt tgggtaaagg tagaaattat gttacaaaca
     1561 taatcaacta ttatccaaat cgtttgccta aaggttcgtt caagaaagtg ggtgacaaaa
     1621 cctatattat aacggaagaa ggaatagaag cattgcgatc acaaacagaa aataaaaaca
     1681 aaaagaagga atgaatatga agagaaattc gcttggtatg gttttattgg catcagctgt
     1741 acttgcgggg ttatcagtac cagtaacctc acatgctgat gaatggactg caaacacacc
     1801 ggatcaaatc caaattacaa caggacaatc aagttatgcg ttcaaaaaag gagatactat
     1861 ttggtcaact gtagagaagc ttaaaagctt aggttacaag actactgttg ctgagtttgc
     1921 cgaattgaac aatgttgatt tggcaaaagg agaacaacgg actatcccga ttggacgaac
     1981 tttcaacatc agtaaagata cattaacagt taaggaagca gatggcgaag tagttagtca
     2041 aactgttcaa aaagataaac ctgatcctat tgaaaatacg gagcaagaga acaaaaaatc
     2101 cgagactaca aaagaatcgg aaccaacagc aaacacttct gataagacag aaacaagtgc
     2161 ttcaagtgtt gatggagcaa gttcatctaa tacaaataat tctacaagtg agaaagaagc
     2221 atcacaggga acaagtgaaa gtagcactac ggaagctgat actggaacat caacaaataa
     2281 ctcacaatca acatcaaatg atgcaaatga atcaaccagt acatcttcta gtgatcaatc
     2341 acaaacaggt tcgagtgatg aaactgacag ttctacttca ccaagtaaac ccgaacaacc
     2401 aactaatgtt gtgctaggcg ttccatttat tagtcaaggt aacacaatgc tttgcgaagg
     2461 aacatctttg ctagaagcgt tacattataa aggtgttgca acaaatcaag atttaatgac
     2521 ttttgttaaa tcaatgccac tatctcctga caataatcca aataatggtt tttctggtga
     2581 atggagacac aatgtggatg gaacttatca aggtatgaat ccggctcctg tagtttcatg
     2641 gggtgttgct aatggcggaa atgttgtaga tatttctggt tcatcggtac aaggattaaa
     2701 agatgagatt gtaaatggaa atcctgtagt agcgtgggta acttatgcct ttgaacctgc
     2761 tcaacataaa ccaatgtttt ggggcgatgc tatttggaat cgacatgtag tagccgttga
     2821 tggatttaaa gacggttttt accatgttgt cgatcctgtg tttggggcaa gttggattaa
     2881 tgcaggatca tttgaaacag catataacgt atctcaatta gcggtttcgg tacgttaata
     2941 aaagatatcg tttatatact aagtagttat atagtcttta tttgtccttg cttaactact
     3001 tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg tgatgctatg aatactagag
     3061 cgttgaaaca gcttgctttc aacgaaaaaa atgattaata ggtgtggtga ttttaactta
     3121 ggaatgggcg cgaaggtcat taccacaccg acatatcgtg gctcattttc ttcgcctaac
     3181 tggtataaag aaggagtaac gagagatgaa gcatgaaaaa acgataattt taaaatcaaa
     3241 aataatttat tgttttgcga ctttagtaat gatgttatct acgcttataa tgcctttttc
     3301 agcaataaga gctgaagcag atgtcacaaa accgacaagt gcaaaattta tagaaactgg
     3361 cgtaaaaaca gacggttata ttagggttaa tatgccgaat catccaaatg aatggatgat
     3421 ttcaagtcaa tttaaagatt ctcatggaaa tattggatat tgtatggatt cagaattgcc
     3481 atccccaaca ggaagtggag ccggaagctt gaagtacaag ggagctggca gtgatgagtt
     3541 ctatcgtatg tttaaaggtg gttttccatc aaaaacagca aaggaactgg gtgcaggaaa
     3601 tgatacagaa gcatggtatg caacacaatt agtttcatgg gttttagccg gaaactttaa
     3661 agtttcacag attgtatggt cacatccaaa ccataccgcc gcagaaacag ctcgtgtaaa
     3721 aaaagcattt gaaaaaattt atgactatgc taagaatgga aaagatacgc ccaacactga
     3781 gttttctatc acagcttcta agacagctga cgaaggaaaa tatcacactt tcacatataa
     3841 aacagcatca aataagactg gtaatgcgaa gctcactttt acatcagcaa aaccagctgg
     3901 tatgaagatt tatgatgcag atggtaaaga aattactaac aacactgtga aattaaattc
     3961 aagttttact attaaagtac ctgtgactac accaagtggt actttgtcat ttaaaggtac
     4021 tgctaatgtt agcacaacca atccttttac ttttgatgga cgaggtgtgt atcaagatgc
     4081 cgtagttatg atcactactt cagaaacaaa ggactctaaa tctttgagtg ctaaatggac
     4141 aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa gatggaaatg ctttaaaagg
     4201 agcggagttt actctaactg acaataacaa tgctaaatct gttaagacaa ccggagatga
     4261 tgggttagct acgtttgatt tgcaacaagg cttaacatac agcttagaag aaacaaaagt
     4321 tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt actgttaata ccgatggaca
     4381 agaatttagt tacactgcta cgaacacaat taaaaaaggt aaagtaaaag ttaaaaaggt
     4441 tgaccaagat ggaaaaccat tagctaatgc tgaatttacc ttgacggatg attcatccca
     4501 aacacaagca gaaaaaacag atgaaaacgg acttgcaagc ttcgatattg tagcaaacaa
     4561 gacctacagc cttgaagaga ccggagtacc aactggttat acaggcgtat tcaagcaaga
     4621 aggaattacc gttaaagatg atggacaaac ttttgaatat acagctacaa acactttgaa
     4681 aaaaggttta gtaaaagttc ataaagtcga tcaagatgga aaacctttgg caaatgcaga
     4741 gttcacttta accgatgaaa aaggtgcaac acaagtagaa aaaacagatg aaaatggact
     4801 tgcaagcttc aatattgtag caaacaagac gtatagccta gaagaaaccg gagtaccagt
     4861 aggttacaca ggtgcattta agcaagaggg tatcaccgtt aaagacgatg ggcaaacttt
     4921 tgaatataca gctacaaata atttgaaaaa aggtgtagta aaagttcata aagtcgatca
     4981 agatggaaaa cctttagcag atgcagaatt caccttaacg gatgaaaaag gtgtaaaaga
     5041 agttaaaaag acggatgcaa ctggtctggc tacgttcaac attattgcga acaaaactta
     5101 caacttagaa gaaacaaaag taccaacagg ttacacaggc gcattcaaac aagaaggaat
     5161 taccgtcaaa gatgatggac aagtctttga atatacagct aaaaacactt tgaaaaaagg
     5221 tgtgatcaaa gttcataaag tcgatgatca agggaaagca ttagcaaacg cagaattcac
     5281 tttgacggac gacaaaggtg taaaagttgt acaaaaaacg gacagaaatg gcttggcaac
     5341 atttaatatt gttgccaaca aaacgtacaa aatcgaagaa acacaaaacc ctaatggtta
     5401 tacagggaaa tggtctaaag aaggcatcac cgttgctgac gatggacaaa cctttgaata
     5461 tacagtaatt aatcaccttg tacctgaaac agtaagaaca ggcggaatcg gtacatcagc
     5521 cttccttctt gtatttctaa ttgcaggatt gagtatggtg ctactgtatt atcgagaaca
     5581 gcacaaagca aaataaaaaa gtgttaatgg aaaagaagta aatatgaaaa aagaaggctg
     5641 acgtatgaaa tcaaaaaaaa ttttttcaaa tttaatgcta tttggtactt tgctaggagt
     5701 gggattgggc gcaagctcaa tctcttctag ccttggacta ggggggcaag tagcatatgc
     5761 acaacaaaac tataattatc aaggaactgg aacgattcat atccataaag taacaaatac
     5821 taatggggat gggaaccctg tctcagatat tgataacaaa gggaaagaaa tagctttacc
     5881 aaatggtgta aaacctcttg aaggcgttga gttctcttat gtgaaactta ctaaagatga
     5941 agctaaatcc ttgggatgga cggatagtga tactacaaaa ggtcaacata ttgatgatgc
     6001 taaagcacga ttattactaa acaaaaaata cagtgaagta agtagaatga agactgattc
     6061 acaagggaat atagacatac ctatacaagt aaatacagac gatttaacag ataacttgta
     6121 tttgattgtt gaaacaggaa cagtcaaagg tgtagttgaa gaagcgtatc ctatgattgt
     6181 taacgtacct gaactaaacg gatcagatga tcaatacttt aattatgatg tacatgtcta
     6241 tcctaaaaac tatttccaat taaactcagc agagcttgat aaaaaagacg aagatggaaa
     6301 ggctcttgcc ggaactgttt ttgaattgcg aaaagatgat ggatctgatc aggttgttgg
     6361 aacttataaa acagacagca caggtaaatt aactgtggat ggtttggaat taggtgacta
     6421 ttatttccta gaaaccgatt tgggaacaga tgctaataga acaacctatt tactagatgg
     6481 taaaaaattc aaatttagcg ttaaacgtgg ggagactgct aaagtagttc ttgatgttac
     6541 caactacaaa cgacctgtaa ttactaaggt aattgataaa gaaaccgttg atactggcga
     6601 agatgttcaa tatacattga aaccatcaat tcctggtaat attggtaatt acgaaaaata
     6661 tgttgtcaaa gatgttctag gtgcagagtt aacttatgat tcatcagaag tgatcgaagg
     6721 aatgacttta gatactgatt acagtatcga acgtgacgga caaacgacaa actatgtctt
     6781 tactgaatca ggtatggata agcttcagaa acaatttgat gcaggggatc gcttctttac
     6841 aattttgatg aacgctcata ctaatagtaa agctaaaatg gatacaccac ttaagaatga
     6901 tgtttcaacg atctacaaca atggatatga acccgaagac gaaacaccag ttgatcacgc
     6961 ttatacaatc attggtgatc accaattcat taaaacagac ggagatacaa gcaaagcatt
     7021 gagtggagca aaatttgtag tgaagaacaa aattgatccg aataaagtta cttacctaaa
     7081 acaagatcca acaacgaaag ctgttacttg gattgcagat aaatctaaag ctactgaatt
     7141 cgtttccgga tcagacggta agttccaaat tgaaggtttg caatatacct atgatgtaga
     7201 ttttgataaa caaggtaatc caacaatcac agataaaaaa atcaatgatt atgctcttga
     7261 agaagttgtt gctccttcag gatatacacc aactagaaac gatgttccat ttgtggtatc
     7321 gaatgatgat gcttctagcg aagtaacaga catcaagaac cttaaaacac ctgaaacagt
     7381 tcgtacaggt ggaattggaa cagtagtatt tctagtggtt gcaggtgtgg caggaacagg
     7441 gctatatgtt gtctacaaac gagaacaaca caaagcttaa tgaaataaaa ttccctagag
     7501 tgtttttggc tctagggttt ctttttaaaa agtgtgtgaa aagtaggtat gagcatgaaa
     7561 aagaagaata tcaagttgat catcttaaaa attgggatgt tgctatgttt aggagtaggt
     7621 ctatatcctg taattactca ggcttatacc tactaccaaa ctcaacaaac actaaactcc
     7681 tataaggaaa aatatgatga agcttctgaa aaggagaatg aagaagtctt caagagatat
     7741 tcagaagtta ataggaaacg aaaagacaca gaacaaacct cagtcacgat tcctgaagat
     7801 aataacaata ttccgtcaaa tggaaatctt cagaaggata aattaggtga attagtgggt
     7861 gaaattctta taccaaagat taatgtggat ttgccaattt atgatggtgt aaacgatttc
     7921 caactatcag caggagcagg aatattaacc ggaactgatt atccaactgg tgggattgga
     7981 aaccattctg ttatatcagc tcattcaggc attcctaata aaaaatattt tacagattta
     8041 gagttgctca aacttggtga taaatttttt attgatattt cagggaaaaa acttgcttat
     8101 caagtagatc ggataaaaac aattctacca accgaatttg aggatttgct tcctgtggca
     8161 ggaaaagatt atgtaacact tatgacatgt acacctgttg gagataattc tcataggtta
     8221 ctcgttagag gggttagcat accttaccaa aaaagtattg ttgagaaaga aaagaagaaa
     8281 gaagatttaa aggtaaaggc gcagatgaac tggtatccta tcgtagttgt aggggtttta
     8341 ttaataatag ctgtgatagt cattattaaa aaaatagtag caaagagacg aggaatgaaa
     8401 aatgaagaat aaaatactac tttttttgac tcttatttgc actttggttt taacaattta
     8461 tcctgcaaat cagctctatc atcaattcaa aacaaaagag aatgtggcgg aaatccaaga
     8521 gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg gcaaaaaaag tcgaacaaat
     8581 aaaatcgaac agcggtaacc aaggattaga caatactcag aaaacagata aaggagtaga
     8641 caatactcag gaaacagacg gtggatcatt tgatattcgt gaagctgaag gaaaggtcaa
     8701 tccatacaca ggagatgttt tttcaaaaaa tcaaacgaaa gattttgaac catctaccta
     8761 ttcaggagcg gttggttatg tctatattcc aaaaataaat gtgctagagc ttttgtttct
     8821 aggcgcttca caggagcatt tatatagagg tgtggcacaa atgagtgcta ctagtctacc
     8881 tttatcaggt aaaggtcatc aaacagtaat agcaggtcat cgtggcggtt atacgtcacc
     8941 acaatttctt tatgttgatc aattagtagc cggagaccac atttatatag attatcttgg
     9001 tgaaaaagca gtttatacgg ttttccgaac agacgttatt agaaattacg gtcagaattc
     9061 aacactgact gatattcctg atttagatat gttaacactt tatacttgtc atccatatcc
     9121 tcagaactac gatcgtttat tagttagaag tctaagagat gaaaaagcaa aatacatcac
     9181 gccacaagta acagttggca ataccgcata cgctgatgcc attgatagcg agtccgtttc
     9241 agataagaca gaaactgtta catcttctaa tgatttattt gaggatgttg atcgtgccaa
     9301 caaggaatta gatggaaata atgatcttaa tattccggaa caaggtagcg tagagatgca
     9361 agacagaacg tttgctgaaa aggcaagttc aatatttaca ggaattacca ataaacatca
     9421 gaaaatgctt ttagtcattt ggactgtaat aggagcatta gcaagtctaa aggttcttat
     9481 tcttttaatc aaagagtttc gaaaagcata atgaagatta taaaaattga tagag
//
LOCUS       DASJTM010000102         9432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJTM010000102 DASJTM010000000
VERSION     DASJTM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.102"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..2126
                     /locus_tag="U4B29_002437"
     CDS             69..2126
                     /locus_tag="U4B29_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEN2107342.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2164..4263)
                     /locus_tag="U4B29_002438"
     CDS             complement(2164..4263)
                     /locus_tag="U4B29_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2107343.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(4564..5388)
                     /locus_tag="U4B29_002439"
     CDS             complement(4564..5388)
                     /locus_tag="U4B29_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107344.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(5385..6140)
                     /locus_tag="U4B29_002440"
     CDS             complement(5385..6140)
                     /locus_tag="U4B29_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2107345.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(6153..8129)
                     /locus_tag="U4B29_002441"
     CDS             complement(6153..8129)
                     /locus_tag="U4B29_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2107346.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(8182..8853)
                     /locus_tag="U4B29_002442"
     CDS             complement(8182..8853)
                     /locus_tag="U4B29_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2107347.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(8913..9122)
                     /locus_tag="U4B29_002443"
     CDS             complement(8913..9122)
                     /locus_tag="U4B29_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107348.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(9189..9428)
                     /locus_tag="U4B29_002444"
     CDS             complement(9189..9428)
                     /locus_tag="U4B29_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107349.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2777 a         1885 c         1490 g         3280 t
ORIGIN      
        1 gactatatcg gttagccgat atataataat catgtaaaca tttttaatta aatttaggag
       61 ttgaaaaaat gaaattattt aatccaaaaa ctgttttagc tattggaaca tttgctgcct
      121 cacttagtct tgctggatct gttgacgctg cttcaactaa cgtcatgtat cgcctttaca
      181 atccaaataa taatgaacat ttttacacct ctagcaaaaa agaacgtgac catctagtaa
      241 agataaaatg gggaaattat gaaggccccg cctgggaagc accgactagc ggtggccatc
      301 ttgtctatcg tttgtacaat aaaggtcttc gtgatcacca ttacacagct agttgggatg
      361 aagtgaaatg gttgactaaa aattacggct ggacttatga agggcctgcc tggcgttctg
      421 ccgaaaaaaa taataaaccg atctaccgcc ttttccttcc tggtgtaaaa tctggctcac
      481 atcactacac tgcaagttgg aaagaagtac aatggttaac caaagactat ggctggaaat
      541 acgaaggtat cggttggtat ggcgctgaca cctctaaacc caaagtgaat aaatcggctc
      601 tagaagctct ttatacgagc gttaagggaa cgaaaaaagg tgactatacc gacaatacct
      661 ggaacgcatt ccaaaccgct ttaaacaatg ctaagaaagt gttaaatgac tcaaaagcga
      721 ctcaaaagca agtggatagt gccaaaaata aattagattc tgcttacaaa ggattaaaga
      781 aaaaaccagt tcctactgtg aataaagctg aattacaagc tttatacaac cgagtaaaat
      841 caactgctaa aggagatttt acagaagaat cttggaataa ttttcagact gctttatcca
      901 atgctaaaaa agtactagac gattctaagg tttctcaaac acaagtaaac aatgctaaaa
      961 atagtcttga tgctgcattt aaagggttgc aacacaaacc tgaaacacca aaagaatata
     1021 cggttactat taaccatatt gacaatcaaa caggtcaagt gattgctaca gatcaagcaa
     1081 aagtaacttc tggttctact tacactgcta aggcaaaagc atttaaatat agcgaaggcg
     1141 ctaaagataa ttttagctac aaagtgattg gtaatgatac tcaaagcaaa acaatcacag
     1201 gtaatacaac aatcacattc aattataatc aagtgcatca aatcaatttg ttgtgcaata
     1261 acaactacaa aaacgaagga cgagttaata ttttgaaaga acagatagtc aatgtcactc
     1321 acggtgacag tgtcacttta actgctcctt ctattcaagg atatgtttta gacgatcggg
     1381 tagaaccaac aaattcagta accttaaaca atgtgactga aagtcaaaat tatattttca
     1441 actacactcg taaatttaat gtgactgtta atcatgtaaa tgctgatacc aacgctgttc
     1501 tctctagtga atcaaaaaca gtatacgaag gggatagctt tgctactcct tggaaaaaca
     1561 tgactgatca aaattatttc ttgtgcagaa atgacgatcc taccgttacc gtggataaaa
     1621 acggccaacg atccattaat aacgtcgatc aaaataaaac aatcacattc aaatataaaa
     1681 agatttcatt agacgaatta cgtaacttta tgcgtcaaaa agaacttgct tggttaaatg
     1741 attatcgcca acaaaatggt gttgccccaa tgcaattcaa tgatattgtt caacaggctg
     1801 cagatattcg agctaaagaa ttacaagtca gcttcagtca ctatcgccct ggtggtggta
     1861 ctttccaaga tttattggaa agcttaggct gctacggtgc taagggagaa aacataagtc
     1921 aaactgggct gtatattgat gaccttttat cagacgatgg tgctatcgat gccatggtag
     1981 gatggaaaaa tagccctgcc cataatgcaa atttacttta taataaccaa agtattctcg
     2041 gattaggtca taattttgta gttgactctg ctggtagaat tggcgtagat aatgtattta
     2101 ttagtgcgaa tccaatcttc aaataaaaga aaacaaggct gattctcagc cttgttttta
     2161 tatttatttg agttttaaaa ctacatagcc caatgatgct aaagtaagaa ataatatact
     2221 tccaataaca gttagcacta aatgattcga tccagttttt ggcacaatga tctgaggtaa
     2281 gttaggtttt accccaatcg ttactacatt agttgctttc tcaatacttt ctactgaaac
     2341 ttttgcttgg ttgtcgatat ccaattcttc attcaaagga gttttagctg taaatatcag
     2401 ctgaatttct cgtccatcta aaagattttt acttaagaaa tcagcggaag cttcccactt
     2461 cagcgttctt gaattgctat cataggttag tgtaccttct gaagtaatat cttttcctgt
     2521 actcatatct atcactcgcg cagaaccttc tttatacgtc aaacgatctt ccactggatc
     2581 ttgaatacta aattgcttgt aatgatctga ggcaatgtct acaccttttg tatgccattt
     2641 ttgtgtgact agatattgta cttcagatcc tgcatttaaa ggagctgtag gcgttccttt
     2701 tttaccatca tttcgaccat caacatcttt cccatcttgt tccacttttt tatttggttt
     2761 aagagagaaa tagttttcta ctgtatttgt tttttgtgtt cctttgttcg gatcattgtt
     2821 aaacgttaca gtagccacat ttggtgcaat tccttttgca tcattttttg ctctcatatg
     2881 gagctgcaaa atataatccc taccgtaatt tgcagcgtta gctaaaaagc tgtcagaagc
     2941 agtataagtg actgtattac ctgatacagt taactttcct cctgtatcag cttttttcca
     3001 agtaccattc gcttcaacat accacaatgc gccatcatag tttttcccat ccgataccat
     3061 atcaaaattt ttgctgtcaa aggtatcagt gattttaaat gatttgtatt tgttcattgt
     3121 gttgactgtt aatgcatcaa acttttggtg aatatgatag taaacatctg atccttgtgg
     3181 aatcactttt ttgtcaatat tgtgtgctgc ctcttcagta ttagctttgt taaacacttc
     3241 ttttgtaggc ttcgatggtt gtggtgtcca tagcaatgca gtatcaaagc taaacatcat
     3301 actattcgta tttgcgccaa tcatgaatgt tggggaagcg ccttgaagcg tatacgttac
     3361 accacttctg tgataagtat cagctcctac aacatcggtg aaatgatcat tcgagctata
     3421 agccttttct gcacgtcctc ccactacttt tgaagttaaa ttgtttgttg ataacgcagg
     3481 agctttgtat tctgcacatg ctgtatctgt tgtgacaaaa taaggtaagc tacccatttt
     3541 ttcataccca acaaattctc tcatatcaat attacgttca ccgacagatt cattattgtc
     3601 attataccaa gacacacgtt gctgatactg tccatttaaa ctgtaaatgg atagaaatgt
     3661 atcgtttaaa tcaataccag ctgctctcat cgatctcatt tttgattgaa tttctggctg
     3721 tggttcactc aaagctactt ttgtttttgt tcctgcatac ttaaattcta gttttgcatt
     3781 catgacattg attgactgca tgaaaaatcc tcgataaata tttttatcaa agagcaaata
     3841 attataatac tttttgccat taattgtttt agctaatccg tcttggttaa ccccagcgtg
     3901 cgctacaaaa ttagaaaaag tcacgtcaca atcaatttct tttcctagat aagtacctac
     3961 tttttgataa tgggcaacga tttttgacgt acttttcaaa tctttgactt ttggccaagc
     4021 aagtactttt tgttttttag ttgaatactc atctagtatc ttaaatccat tgatcccagt
     4081 tacttcgata tttctcaatg aatcactatc atgataaagc accagattat tattttcttt
     4141 gatttgactc ggatcaactt tttctaaggc ctggactgca gttcccctcg caaaaagaag
     4201 aaggaacaaa aacaacacta aagtaattac tcttcccgta gtgcaatgaa ttttattttc
     4261 cataatgccc tccatctaat atttttttaa acgatcgtaa gcttttcatt ctttttttcc
     4321 taatacccac gttaccaatc ctgtaagaag aaccaaacta accataaaaa ttggattgat
     4381 caagagcttt ttaatcacct ctaatggcat ggaagcacat tgaatgttta gaaaatgaat
     4441 gaacaatcgt tgatatcttg gataaattga taattgagaa aaaattaaac cgcaacacaa
     4501 tattgttgcg gctaaaaagg atagattaaa aactaatatt cttattattt tcatcatttc
     4561 atcctaatta tcaaaaaata attgagcaaa aaattcccaa caattttcaa aaaagttctt
     4621 ctttacacca gttgacacaa cttttttaat ttcttctttc ggctctgatt tctctgattt
     4681 agggtataac caaacatccg tcaaaaattc acctttttca tttgaaacag gtgtcaataa
     4741 aacaatatct tcactgtttg aaatgttttc agggcttgat tcattaacga tatagtatgc
     4801 ttgttttttg tttgcaggta cttcaatgga aaattcaatc acgccttcct gatcatcaat
     4861 cgttttggtt tctccttcag ccactttttt caactggtcg tatgaaagtt tttttgctcg
     4921 atctttcaat tttgactcaa ttatttctac agattgattt ttctcattat catcttgtga
     4981 catttcttct aagatattcg tgatatcgta gacaataaac ttcgcgccat tgataccttc
     5041 ttgctgggct acttgactgc cttgattgtt gatttgattc gaagaaataa ttttttgaat
     5101 atgaatggct tgcttgatca cttgcgtatc ttctacgtct tgcggctttt ctgaagtctc
     5161 tgaagaactt tctgacgttg gattcgttga ttcagtaaag cttatatttg aatctgccgt
     5221 ttcttctgta gtattctccg aactgtccgt ttcgcttggt gcagtaattg aattatctat
     5281 tgtttgagtg gtactactat cactgctgtc agcgaaagac agttgcgaat aagctacagt
     5341 agaaaagaga gctgctacac aaacaaacag ccattttctc ttattcattc cgatctcctc
     5401 cttatttttt tataaataaa aataatagga acgcaaaaaa aagcgaatag cagtgctaat
     5461 cgccaatcca atgaaattga tttcctattt tttgcttttt tctgtagttt taaatcttct
     5521 ttactgaagg aaactcgttc accatacact aaaaagcggt gtgagttgac accttttggt
     5581 gtgcatgtca ttaacgtgat cctatcttgg tctttttcag gcataagata atctaaataa
     5641 tttttttgta attcttctgc tttaacttct tcaattcgat aaatttgata ggcatgttgt
     5701 tcttcattta ccttaataaa gaatttttgc ccttttttta ctgaaggcaa atcatcgaaa
     5761 agattatctt tataaagtcc actgtgcccc gcgattaaag gatttttacc gtttcctcct
     5821 gtaatatcgc cagttccttc cgtaatccct acaccattct ctaaaatttt gtcggaagtt
     5881 ccgtcataaa ttggtagttt taaagaaata cttggaattg taagaacacc aacaggatcg
     5941 cctaactcta ttttcgcaac atccaaagaa gaatctgttt tttcaagatt ttcaatggct
     6001 aaaattcttt gctgttgttg aaatttcgct acatttgttt ccacaactgg aattaaatag
     6061 actgctactg ccgacagcaa ccaaaaaaat atcatcagtt tgtgcatgac tcttcttttt
     6121 gggggcttac gaataatcat tagctgatcc ttctaggctt tcttcttaaa gtaaacaact
     6181 gcagaaagtc cccctgctcc tagcaacaag caagagatca atacgtaaga ttggaaaccg
     6241 ttgctaccag tcattggtac ggaagctttt tgattatttt tgatcgtgat tcggttttct
     6301 tctttgtatg aatctttgtc tactttgaaa gagatctctt ttgttaattt ttgatagttt
     6361 tcaggagctt ttgtttcttc cagtgagtaa tctccttcag ttaaaccagt gatttcaaaa
     6421 tgaccctctt tatctgattt taggattgct gcatttgcta catcgtctac ccaaactgct
     6481 tctttgacac catctttatc tgtatcagta agtccaccgt agtacttacc ttctgcattc
     6541 ttagctgcaa attctgcgcc ttcaagcggt gttgctttga tacctgttcc aaataggccg
     6601 ctatcgactt ttaagaattt tgcgccataa gtcacaacgt cagctttcgt tttttcgtgg
     6661 tgttcttcct gattaaatgg attattgtta taaacgaaat ccacgctatt tgttaatgga
     6721 tttgcgattt cagcactatc attcacaaac atgttataag tcatttttag atcttgacca
     6781 tgtaaattcg caactgtatc acttacttgt ccattattta cgataaaatc gatcacaaaa
     6841 ccactatcag aattttcgat tttatagtca gtattttctt ttaatacgac tgaaccatca
     6901 ttatttgtta ctttaatatc tgctttttca gcaacataag aaagtccttt acctgctgta
     6961 tctccccatt taaatacact gtaaggagaa gtttcgcttt tcacaccgtt tactgtgata
     7021 ccgtctgcgc cacctgcaat gtctgttgga atatgaatcg ttccttgata atttactgcg
     7081 tcaccaattt ggaaagaatg ttcttgtcct gttccatttg ttactgtttt atcgatcact
     7141 ggtgctttat agtttacgta tgaagctttt aagtcacttg tcacgccatc attcgtaatt
     7201 tcaaaggtta atttattgtg cgcatcgttg cgagcgtctg cgcctaataa atattgatct
     7261 gctgttggtt cttgatctga tcccactaca acttccgaag gaacttccac aaaatagtat
     7321 tttcctaacg tcaaatcagt cgcagtcagc tttccttgat catccgatac aagatcaccc
     7381 aattttgttc cttttccagg ttcgcccttg tataactcaa attttgcccc ttttaaaagg
     7441 gtttgtttgc tttggtctgc gccatcgtca ccaaatttcg ttaattcaaa actcaactta
     7501 gaaacaatgt tcttaggata caaatgaata ttttttaaaa atcctgatcc tgttttatct
     7561 gtcattggcg caatcacaac cattggtttt gctttttgtg tgactaatcc agcagcggat
     7621 ttactttcat aaaccacata tacattccct ttggaattga cataggctgg ttgatcagta
     7681 aaagtgatct caccattttc atctacagct gaagtaattt tgttggttgc attcttgacg
     7741 tactcactgt tagatccctt gtcttctaca tcttgtacaa tctcgtccac tttcgcgttg
     7801 gttagatcag aatcttctct cggtaatact tgatccgtaa tatttcctaa cgtaaactca
     7861 acatcaccat aatctgcctt gttgtatttc gttacacctg taggatattg atcgtgcgta
     7921 taaccatcgt ttttgatccc gtcaatatca acattcaatt gcgttccttg atcgtacatt
     7981 aatttatgaa ccgtgatatt gaccttctct ggtggtgttt tttgcgtgac atcttctgca
     8041 tgtacttgat acccctgaaa gcagcctgca atgattccta acattccaac aagcattcca
     8101 gcttttttta cataccgatt tattgccatt ttttactctc ctcatttcat atttcaacga
     8161 ctcgcgttga ttaaaacctt gttattgtag ctctccctgt accacgtatc tttcttttcc
     8221 ttgtgacgta taaacacaag taaacaacgt taataagggt ttctctcctt tctttggcaa
     8281 agacaagaaa gagttctctt caggaacgcc atcgattaat ttaaagtacc caggtacgat
     8341 aaatttacgc gttaccttgt aacgatagac gccatcgtta ctaactaagt ccatcacctc
     8401 ccctaatgac aacttttgaa tatcagaaaa taaaacacct ggcatttcca tgttatgacc
     8461 tgctaggaca tagtttcctt tgcccatttc ggcatcttca taaaaataag tagccgctcc
     8521 aagactcaaa gtgtattgat ttgccccttt aaaaattggc aatgctaaac caagtgcagg
     8581 aatctgaatt tgtgcaaata cttgtttatt gacgcccatt tcgattgctt ctttttgtat
     8641 ggacaacatt tgccccaaag acatatcatt attgacgttc gttgcatcct ccttataact
     8701 ctttgcatgt gcattgattt gatctacggg catctttttt tctatatttt gcttaaacac
     8761 ctctccttca tgtttttggt agctaacata gccaaacaca ctgcaaaaga cgattaaagt
     8821 gactcctaaa aaaccaaata accatttttt catccatctg cctcctttca gacaaaacaa
     8881 aaaaactttt tctcatttcc atagaaaaag ttttaaaaat gaattctata agttcgtctc
     8941 aactgttcaa agtttttcat tgaacctaac aaacaagctt tctcttttcc tgttaactgc
     9001 atatcattga tatattgcgc gacagataat cttttaaggt gtttttgctt catcgttttt
     9061 agtaactttt cttccatttc tttcgacact ttttttctgt tttcgatttc ttcataattc
     9121 atgagttttc ctaccttcct tgttttttgc aaaaataaaa aagcctatga tcacatacgc
     9181 tctacacttc agtcttgggt tatctttttc tttcgtttaa ttactggccg tgctggtttc
     9241 aaatccttga ttccattatg ggttctccaa taatcgaatt cttttcgcaa tctttctttt
     9301 tccatatctg ttcctttaga taaggctttg aatacttctt taggtgaata accacgagct
     9361 aacagctgta cataccttcc ttttgtcaaa gtatccatat ccatcgcctc taaaactggt
     9421 cgcgccatct ac
//
LOCUS       DASJTM010000103         9432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJTM010000103 DASJTM010000000
VERSION     DASJTM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.103"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..680
                     /locus_tag="U4B29_002445"
     CDS             <1..680
                     /locus_tag="U4B29_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107350.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="U4B29_002446"
     CDS             800..1000
                     /locus_tag="U4B29_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2107351.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="U4B29_002447"
     CDS             1076..1273
                     /locus_tag="U4B29_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107352.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="U4B29_002448"
     CDS             complement(1570..3117)
                     /locus_tag="U4B29_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN2107353.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="U4B29_002449"
     CDS             complement(3201..4004)
                     /locus_tag="U4B29_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2107354.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="U4B29_002450"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="U4B29_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN2107355.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="U4B29_002451"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="U4B29_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN2107356.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="U4B29_002452"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="U4B29_002452"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN2107357.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="U4B29_002453"
     CDS             complement(6389..7318)
                     /locus_tag="U4B29_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2107358.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="U4B29_002454"
     CDS             7513..8196
                     /locus_tag="U4B29_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN2107359.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="U4B29_002455"
     CDS             8357..9400
                     /locus_tag="U4B29_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2107360.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DASJTM010000104         8902 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJTM010000104 DASJTM010000000
VERSION     DASJTM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.105"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..254
                     /locus_tag="U4B29_002456"
     CDS             <1..254
                     /locus_tag="U4B29_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107361.1"
                     /translation="QEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKGIYEMKVKI
                     NKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(351..785)
                     /locus_tag="U4B29_002457"
     CDS             complement(351..785)
                     /locus_tag="U4B29_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2107362.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1017..1727)
                     /locus_tag="U4B29_002458"
     CDS             complement(1017..1727)
                     /locus_tag="U4B29_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2107363.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1717..2577)
                     /locus_tag="U4B29_002459"
     CDS             complement(1717..2577)
                     /locus_tag="U4B29_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN2107364.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2582..3220)
                     /locus_tag="U4B29_002460"
     CDS             complement(2582..3220)
                     /locus_tag="U4B29_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN2107365.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3235..3534)
                     /locus_tag="U4B29_002461"
     CDS             complement(3235..3534)
                     /locus_tag="U4B29_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107366.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3577..5061)
                     /locus_tag="U4B29_002462"
     CDS             complement(3577..5061)
                     /locus_tag="U4B29_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2107367.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5081..5542)
                     /locus_tag="U4B29_002463"
     CDS             complement(5081..5542)
                     /locus_tag="U4B29_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2107368.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5560..6627)
                     /gene="ulaG"
                     /locus_tag="U4B29_002464"
     CDS             complement(5560..6627)
                     /gene="ulaG"
                     /locus_tag="U4B29_002464"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN2107369.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6845..7615
                     /locus_tag="U4B29_002465"
     CDS             6845..7615
                     /locus_tag="U4B29_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2107370.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7888..8715)
                     /locus_tag="U4B29_002466"
     CDS             complement(7888..8715)
                     /locus_tag="U4B29_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107371.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2704 a         1870 c         1389 g         2939 t
ORIGIN      
        1 cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg
       61 agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga
      121 tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg
      181 tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta
      241 aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg
      301 tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca
      361 ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc acccatttcc
      421 tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc
      481 aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc
      541 actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg
      601 atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc
      661 tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa
      721 ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata
      781 ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat
      841 ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa attcctcttt
      901 ccttttagaa tgcatacttt ttgataaata aaaaggttgt gataaatttt cttcacaacc
      961 tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat
     1021 tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc
     1081 gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca
     1141 tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca
     1201 tgtccataga caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc
     1261 tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat
     1321 tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc
     1381 aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca
     1441 gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt
     1501 aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc
     1561 atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact
     1621 tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct
     1681 tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc
     1741 cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct
     1801 tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct
     1861 taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga
     1921 atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc
     1981 tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg
     2041 atatacttgt agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga
     2101 gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc
     2161 ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc
     2221 ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg
     2281 gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa
     2341 cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat
     2401 ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc
     2461 cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg
     2521 ttctttccat gaaatatctt tgggtaacgc tttttcataa atcccaattt gagccatttc
     2581 cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat
     2641 ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt
     2701 gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt
     2761 ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc
     2821 tttgatggta gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag
     2881 tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag
     2941 ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac
     3001 tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga
     3061 acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca
     3121 cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt
     3181 cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt
     3241 gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttga ctgacatcac
     3301 atttttgata ccgataactg taactccttt tttctctgcc tgagtaaaca tattcaaaaa
     3361 attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc
     3421 acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct
     3481 catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat
     3541 ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata
     3601 tgtgtcatag tcttctacaa tcaaaaaata accatcagga tttttccgat attgtaactg
     3661 cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat
     3721 gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccgta
     3781 tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga
     3841 aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat
     3901 aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa
     3961 taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga ccccaatcgt
     4021 taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc
     4081 tggtaaaatc gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg
     4141 aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta
     4201 gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag
     4261 tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc
     4321 attgaaaatc gacaagaatc ctggtaattt gatatcttca atttttttgt cttgcttgat
     4381 tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa
     4441 gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt
     4501 gattcctact gttaagttag aagctactgt ccaataacaa cctaaaatca gtcccataaa
     4561 aatcaatgct tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt
     4621 agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt
     4681 ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc
     4741 tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata
     4801 aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca agattggccg
     4861 aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt
     4921 taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac
     4981 gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat
     5041 gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca
     5101 caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca
     5161 ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcga
     5221 gtgtaaaaaa caaactagcc gttttctcct cattctcccc ttcactttca aaacggattg
     5281 gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag
     5341 aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat
     5401 ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc
     5461 tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat
     5521 ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg
     5581 tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc
     5641 atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc
     5701 atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg gatcagccat
     5761 aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc
     5821 catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata
     5881 ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta
     5941 atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc
     6001 aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt
     6061 gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac
     6121 tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt
     6181 gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc
     6241 ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt
     6301 ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg
     6361 aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg
     6421 ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca
     6481 aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc
     6541 gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt
     6601 tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt
     6661 acaagtaaat attaatgtgt atcagcatcc atgaaaaccc ttttatatga ccgtttgttt
     6721 tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa
     6781 tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa
     6841 agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct
     6901 tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg tatctattag
     6961 tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg
     7021 ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat
     7081 taaacaagcg attgccagag aagctagtaa atacattaac gattatgata cgctgtttat
     7141 caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt
     7201 cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt
     7261 aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac
     7321 gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca
     7381 tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac
     7441 aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa
     7501 gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt
     7561 attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc
     7621 aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg
     7681 aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtatccatc caatcgatac
     7741 catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa
     7801 tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca
     7861 aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc
     7921 gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc
     7981 atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata
     8041 agtcgattta tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc
     8101 ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag
     8161 attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa
     8221 atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg
     8281 gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg
     8341 ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc
     8401 agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt
     8461 tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt
     8521 ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt
     8581 gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc
     8641 ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc
     8701 atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct ttatatcata
     8761 cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca gaataaaaat tttaatttta
     8821 aaataatatt caaattaaac aactggcgta tctgctcctt gatttctttt tgtgttatct
     8881 gaataattat cgtttacagt ta
//
LOCUS       DASJTM010000105         8834 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJTM010000105 DASJTM010000000
VERSION     DASJTM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.106"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..857
                     /locus_tag="U4B29_002467"
     CDS             54..857
                     /locus_tag="U4B29_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2107372.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1442..1897
                     /locus_tag="U4B29_002468"
     CDS             1442..1897
                     /locus_tag="U4B29_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107373.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2016..3074
                     /locus_tag="U4B29_002469"
     CDS             2016..3074
                     /locus_tag="U4B29_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEN2107374.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3116..5065
                     /locus_tag="U4B29_002470"
     CDS             3116..5065
                     /locus_tag="U4B29_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2107375.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5068..8709
                     /locus_tag="U4B29_002471"
     CDS             5068..8709
                     /locus_tag="U4B29_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107376.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3181 a         1282 c         1832 g         2539 t
ORIGIN      
        1 gtaccagatt aacatgatta aaggagcgca aaaagaagat tggagagtaa tcaatgtctc
       61 aagaagttac cgaaaagatt attgatagtg atgtagattt agtatcagaa caaacaaatg
      121 caattgtgcg ttataacaat ggtctgaata caacagcttt gcgaggattt aagcctgttg
      181 aaatggatgt gttttgggct attttatcaa aaatgaaacg tcaaggaaca gcagaagtaa
      241 cttttgattt caacctcttt cgttatttgg tcaaatacga cagaagagaa aaggacagtt
      301 tttattctgt gttaaaagat ttgagtgaca aactaggtaa tttaacctac cgttttgaag
      361 attcagacgt ctatgaacag ctgtggcttt ttcaaaaatt ccggatagat aaacagaagg
      421 aaacagttac aattaaggca aatgaggaat ttgaatttat cttaaactca attggcaaaa
      481 actttactcg ttttgaactt gataatttta caaaactaag aagtagctac acaaaagaat
      541 tgtatcgtca tctcatgcaa tacagaaata aagagtggcg aaatggttat tgggtagtca
      601 aagttgaaga ttttagaaga gcattaagta taccttcaag ttatagaatg acaaatattg
      661 ataaaagaat actagagcag gcgaaagaag aatttttagc tcctgatgaa aacggaatac
      721 aggtatttga aacttttgat tatgaaaaaa tctatgcaaa gaaaggaaga agagttgatc
      781 ggctcagatt cacttttagt gagccagaat caaacttgcc aaccatttca atgcataatt
      841 ggttagaaga agattgagaa gaagcaggtt gtaattttaa tacaacctgc ttctttaaaa
      901 aagtatgggt aacacttttg gacaataaat gcaagaagtt tttttgtaag caagtttcag
      961 tctaaataat atttgaaaga atggctaatc tatacaaatt ttaacaacta tgggtaacac
     1021 ttttggacaa gtcaagaaaa aaaacaattt ttcttaattt gttgatcgac taaaggtaac
     1081 acttttggac aacacagaat aaaaagtccg gtgatatcag ttttggtagg aaacatctta
     1141 atatctttgt gagattgaag gtaacatttt tggacaagtc aactatttgt taaaagttaa
     1201 tatagagtaa ttagataagt tttttctttt cgtctgataa atggataata ttagttattt
     1261 tattgctaat tagtctttca tgatgactga aatagctaaa ataaatatct cgttggataa
     1321 ttgctgatac tttaagggat atctaaattt gctgattgat ttatttatgt tattatgata
     1381 ttataataac ataaatgtgt tacgcccgaa cctcatttta aaaaaatgaa ggggtgagac
     1441 aatgaatgct attaattggc atgaatttgt taactatatg gagagcttac tagatgatct
     1501 aaaaagtttt agtattgctg atgcagtaaa gcatgggtta ccagttagta caacgatgaa
     1561 attacgagga ttgaaaattc cagatgaaat tattaagctg agaactaaaa caattattga
     1621 aaccgcaaaa gtcgttgaca atatgaacca tgaactaaaa ggcaagaaaa caacttacga
     1681 gaaagatttt gaacgttttt taaaatctgt tattggaaaa tatctaggta aaaaatcttt
     1741 cagtgaattg agagatgaag agattgtacc tactccaatg gttagcaaaa tccgaaaaaa
     1801 caaagccgag gggaattact atcttggtac aatagcaaaa atcattgatt atttggaaaa
     1861 taaagagcaa aagaaaggaa cgaagaaagg aagataattt ttgtgttaaa tattaatggg
     1921 gaagaatata ccgaaatgat tcaaaatccc gaacctagaa ttaccgaaaa aacgtctgat
     1981 tgtttgctct tttatgaaag ggggcagaca aactaatgga aaaaaacgtc aattcaacat
     2041 ctaccaaaga tcaatacagt attattggag ggttgcttgg tgataaggat aagagaacac
     2101 gtcacggatt acgcattatg cttgcaactt ttctaggagt gcttttttta ttaacaggct
     2161 tgggtagcta catttatcaa ttaggaaact ataattatta tctcgtgaac aatacaacac
     2221 ctataggctc ttcattggag tttgctcgat caggagcaga gatcactatt tcagatgtgt
     2281 ggacagatca aaacagagat gtaacagtag taaaactcgg ttactcgcaa aacgctagaa
     2341 agttgttatc tacgcaagga aaaaactaca acttatatat ggcaacgaaa gaaaaagatc
     2401 gtcctaaaaa gcttgaaatg agctatggga tgttaagtac tgatggggat gctttcttat
     2461 tcctaaaagg taaattagat gaaagagctt atcaagtttt tattgcaaac caagtatcac
     2521 taatttctgg tgatgataca gctgacgatg ataccaaagt caatgtgaat gaagcagatt
     2581 cagttacaaa agcactttct caatattcta tgaatgatgt taataatgaa ggagtaattt
     2641 cttttgggaa aaaagcaaaa cctgttaata ctcctgataa tatcaatttc agaataaatc
     2701 catattcaga ttcaactaat gtctatcaag gaagctttct taccaaagat ggcgatattg
     2761 attattcaaa agttgtagca gttacaagta agaataatgt tgtaagtaag ttagaagatc
     2821 agttaaatgc ttcaaatact aacatcaaga aaattgaagc tacaaaagct gagtatctgg
     2881 aacgtttaca acaaaatcct gacgataaag attcgcaaga aagtattcgt caactagaca
     2941 gtcgaatgga gaccgaaatt aaagcaatca atgatacaca aaaattgatt gatcaataca
     3001 aaaatgctga ctttaacaaa gattcttttg gagacatgaa gacgacattt aagtacctaa
     3061 ctctgactga ctagaagtaa attattctta ttttagagga agtgaacaca aacagatgga
     3121 aaatagtacc gagtttttta agaatattca aactgaattt aatcatgctc tcacaccaat
     3181 gaaaccaccg aaacataagt tagtgggacg tgatgacgaa ctccatgagt tgtcactgat
     3241 cttgaataga ccggaaacac cgcaagctat cttgttaggt gaagccggaa caggtaaaac
     3301 agcacttgca agtgaatggg ctgaaagaca gcgacaagat ggaaagaaag tacaagtata
     3361 caaattatca atcggacaat tgcgtgttgg gggagatgat gtcctccaaa cacgtttaga
     3421 aacattaatt ccaagattga aagaatatga gcgaatttta aaatctgagg acaaagaaaa
     3481 tgaatttgtc ctcttcattg acgagtttca taaagttgtt agctcttttg gtagtggcaa
     3541 taaaattggg ggtgattcgc taaaagaacc gttagcaaat gaatcacttc aattgattac
     3601 cgctactaca agaaaagaat atgaaaacta tatcaaaaga gatgatgcct tagggagacg
     3661 gttacaaccg ttagaattga atgaactaaa aagcgatgtt gtcgtcgata ttttgagaca
     3721 atggctaaat acatcctctg ttaaagatga tcctcaggct tcttttaaat tatcgcaaag
     3781 gattcctgat gaattacttc aagaaatcgt acaagcaaac aagctttatc gtgaggattt
     3841 gtccgaaccg gctaaatcaa ttgccgtaat ggaggatttg attgcaaaag atcgagtaga
     3901 cggaaatgct tataatcgag aaagtctatt gcaaatattt ggtaagtata atattcagct
     3961 agattctgat tttgatatgg aaaaacttca agaggaatta aagtccataa aagggcaacc
     4021 gttagcagtt tatactataa caatgttgtt taaacgtatg aagcttggat ttgatcgttt
     4081 taaaggtcga aagcaaccaa aagcaactgc tctgtttaca ggatcaacag gggtcggtaa
     4141 aactgcaatg gctaaagccg tggcaagagg actttatggc gatgaaaata aagttctctt
     4201 agttaatatg ccggactaca aaacaaaaga tgatgcaatg cgttttagac gactcatagg
     4261 tactcatgta aggcatgatc cttctgcgat tattgtgtgt gatgaatatg agaaagctgg
     4321 tgtagaaaca ctcgatgcct ttttatcaat tttagatgaa ggtaggataa gttttactga
     4381 aagaggatgg gatgatcgag aagttcacga cactgtttca ttgtcaaata caattttatt
     4441 tgcgacaaca aatgcaggtg cggagatgtt cgattcgtta aacagatata gctcactatc
     4501 tcgtgttaca aacaaggaaa attctaatga atatagcact gaactaaaaa gagaataccg
     4561 aaaagtcgca tcagggatca aacaagcctt gattggtgaa ggtctaaagc ctgaagttat
     4621 taaccgattt ggacgaatag ttccattttg ggctttatcc gaacaaactc ttctggaaat
     4681 tgcttataat gaaatgttga agagtgctga attagtacga aaacatttaa atatagattt
     4741 acgtcttcca cctaggaaag aaagagtata tggtcaaaat ttttatgcag acgatctctc
     4801 catgcactta gtttttgaca tggcttcaca agaggattct aaatcagggg gtgcaaggaa
     4861 tatcttgcat ttagttgaat ctgagtttga agaagcgatt actgaagcaa taattactta
     4921 tccaaacgca aaaatattcg atgttaagtt gagtgaagcc ggagcgtttg aaggaaaagg
     4981 tgattctaat gctgaaatta ttgcaaagcc ttccgttgct gaaagtaaag aaaacaagat
     5041 tgtattttcg attggagggg cgtagctatg aaattcaaga aatatttact acaaaacctt
     5101 agaataaaac gatgggtcgt aagaggtcta gttattggag gagtattagc agttacttta
     5161 ggcgctcctt ttggaatgaa tgatccaact cctgacaatc aagatagcaa acaagaaaca
     5221 gttataaata agaacgttgc atatgcggaa agtaaaccgg cagaacaagg aatgtggaca
     5281 ctatatgctc gtatcctaat gggagataaa gaagttaatg atagtgccgc taaaactgga
     5341 caaatgaatt tgttaggaca aggtggtatc agtgccgatt tcccttattc tgacatttct
     5401 aaacaaggta attcgattgc agggggtaat gcaggtactc aactagcatc tactttagca
     5461 acttatagtt actacggata tattgaaaca gtctcaggaa atgctattgc cagtcaagca
     5521 tcatccattc taggaagtat tggtaggacg attggtagtt ttatcggatc aattagttta
     5581 atcctttcta atattgctga atccataaat gaactccttg gaaaattttt catgagtatg
     5641 aatatatttt ctcttatggg gtggggagaa ggtggatcgg atgtatctaa tccggtatct
     5701 aaagcattaa ctagcttgct taacaatttg ggattaaata atgaattatt taaagcgata
     5761 gcaagcttat ctatgctctt tttcacagca atactaggtt atcaaggaat gaaattattg
     5821 tcgaaagatg gtgttaactc gaagggatta tcaccagtac ttgaaaaatg gattgttcgt
     5881 atttttgtat tgtttatatt cttaccagtg gcaggatcgc tattgggagt ttatatcaac
     5941 ggcatccagt cagcaaaaaa agatggagat ataccaacaa atgttgtttc tgactacatc
     6001 attaacacaa gactttgggc ttctactcaa aatcttgctc catcatctga tactggtggt
     6061 gatgtacctg acggaaagcc tacatccgaa atcaagttcg ttgatacaga ttatgaacca
     6121 tctaaaaaat ctgcaatgat aagagcgatc aatactagca gttatgccag acaaaataat
     6181 gatctagatt ctaattcagt cggtatggct ctattaaaag aatggtcatc gaatgatact
     6241 ttcaatatta atacttatgc cggtgatgtt aatgtcgccg aaaatgatgc ttatatggga
     6301 agagcgcctg ctgtttggac aggctataat ttaggggatg gaaaagaagg aagtgcaatt
     6361 aagaaaccaa ccaatttaaa tggatatatt tggtcagcaa atcaaaatgt gacagacgac
     6421 aatcgcaagc ctgatggaaa aggaacagcg cataactttg atgctactca accttggggc
     6481 gttgctaaca atgcaacttt tagcacacag tcggtagctt tattgctcca atcggcactt
     6541 gatacatcgg gagctaagtt ttatgcgtat aatatcgctc cttcaggaat tcaaggcagt
     6601 gctaagaata tgtcaacagt aaaaacagaa tggcgtacag catccttagt tggtcatgga
     6661 gctatgggta agtttggctc gtggctagcg atgaacgctg aatcaatcgt tttagcagtt
     6721 ctttacttag catgtgcttg ggcgatgttc aatgtgaata tcgtgaaagc atttaaacgt
     6781 gaatttattc ggatttggga agtagttcta acaggatcac ttgcatcagg agcaggtgtg
     6841 atggcaattt cattaggtgt tgttacaaca tctctgatcg gactacaatt acctatcttt
     6901 ctgattactt ttattcgtgc atttggtgat actgctgtgg gattcattcc taatacatta
     6961 gttggcgatg gcgtagcaaa tattgttcga tctgctatga ttttaggtgt aggatatctt
     7021 ttagtcttta aaaaaggcga aaaagtaaat aatttgatta atatgcttat tggcttacca
     7081 cttgaagtag cgtttgggtt tgatgaacgt gctaagttat tatttggtaa tagaaatgat
     7141 gctgtgcgag ttgtggacgg cgtttctaaa gcaatcaaga aaagccaagg agctaaaaat
     7201 ccctattatg atggtggcaa cagctcagta aaagatatgg ttgcaggagg tcttggagca
     7261 gttggagcgc aaaaaatggc tgaacgtttg aacaatatgg gagatagcaa tgctgatgct
     7321 aagtccattg ctgataaagt aattcctgaa acaccagaag gaatgtcatt caacggtggc
     7381 ggagttggtt ccacaatcaa taatacctct ttcaaagatt tagttcgtca agatcaacta
     7441 cgtaataact cccaacaata tattgatcat ttaggacgta tggttgatga aaacggaaac
     7501 tttgtcgatg aaaatggtga aatattaaaa gatagtaaat acggtaaaga tgctaaaggc
     7561 aactttattg acgaaaaagg gcgtttggtc gatgaagatg gaaaccttat agatactaat
     7621 ggtaaaccat tggaagatag tgaagatggt aatattggta tggatagcga aggacgttta
     7681 attgatgaaa aaggtcgctt agtcgatgaa gatggaaatc ttgttgacga aaatggacgc
     7741 aggttgctaa atggtgattt tggtcatgat agtaacggac gtttagttga cgaaaaaggt
     7801 cgtttagttg atgaagatgg aaatcttgtt gatgagtttg gtaataaact tggtaaacac
     7861 agcgatggat ataatgtgaa tggagattta gtcgatgagg atgggaatat cattgagacg
     7921 agagatgcgc ttgtggcaga tcgaaataga gaactcgaac aacaacggca tacgccaatt
     7981 gctgttgcaa ttcctgagaa tgaagaagac gagattactt tagatcaaac tcatcaagaa
     8041 ctgaggaatg aaatgagtgg tgaggacgtg tcagacagag tgcatcggga attaggagat
     8101 ggagcatctg gtgaaagtca gtcaagagag cttaacagca actttgataa tagtgctatt
     8161 cggaatgaca cagtacttaa agacagagaa tcgctaacaa aagaaacgga taaagaacaa
     8221 tcagatgtga agaaaaagtt cattcctact cctaatgtta atcaaacaga taaatatgaa
     8281 acaaatattg gatcaggaat gtcgaaagac aatcaatcaa ttagagaacc tgaactgaca
     8341 aataaaaata atagaacaaa taattctaaa tctacttcag ataattcagg gaaatctaat
     8401 caatcaacca agaaaacatc aacagctaaa tcagccgttg aatatctatc aaaaactggt
     8461 aaaaatgttt acacctcagc agataaagct ttagcaaaag ctccaaaagg tagtctagca
     8521 gggataacct atgaagctaa aaacgaagct attaaaatag gtaaaggaac ggctaaggtt
     8581 gccaaggcta ctgctacagg agcatccaaa actggtaaag tagtgaagaa atctaccatg
     8641 tcagtcaagg actatattgt aaaatacggt aatcaaaaat caactgaaaa taagtctgaa
     8701 gacgagtaaa aagaaggagc attgatttgc tccttctttt tactttgatt tctaactgaa
     8761 gatgtcaagg aaaatttcta tttatgcaaa aaaattattt taaattgtct ataaatgaat
     8821 taaatattta ctgt
//
LOCUS       DASJTM010000106         8815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJTM010000106 DASJTM010000000
VERSION     DASJTM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.107"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(413..2794)
                     /locus_tag="U4B29_002472"
     CDS             complement(413..2794)
                     /locus_tag="U4B29_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2107377.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2784..3431)
                     /gene="recU"
                     /locus_tag="U4B29_002473"
     CDS             complement(2784..3431)
                     /gene="recU"
                     /locus_tag="U4B29_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN2107378.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3495..4034
                     /locus_tag="U4B29_002474"
     CDS             3495..4034
                     /locus_tag="U4B29_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN2107379.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4124..4543
                     /gene="gpsB"
                     /locus_tag="U4B29_002475"
     CDS             4124..4543
                     /gene="gpsB"
                     /locus_tag="U4B29_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN2107380.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4565..4932
                     /gene="rnpB"
                     /locus_tag="U4B29_002476"
     ncRNA           4565..4932
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4B29_002476"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5141..6307
                     /locus_tag="U4B29_002477"
     CDS             5141..6307
                     /locus_tag="U4B29_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN2107381.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6353..7849
                     /locus_tag="U4B29_002478"
     CDS             6353..7849
                     /locus_tag="U4B29_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN2107382.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7930..8613
                     /locus_tag="U4B29_002479"
     CDS             7930..8613
                     /locus_tag="U4B29_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2107383.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2788 a         1588 c         1861 g         2578 t
ORIGIN      
        1 gaataacgag gtattgtttt cgataaaaaa agatggtgca gacaaattat gataattaag
       61 catactatca gataactcaa aatgaagcct tctttcaaaa aattattcaa aaccatatat
      121 tgtatttttt atttgataat aacaatggga ttattaagaa gtggcactaa ataaaagact
      181 agtgtgtatt tagccgattt aattataaac aagtaaatgg ataaagtata tgtaatgata
      241 aaagaaaaat ggattttcta atttttaagc taaatgttag atcacacatg tatatgctgg
      301 atagtcttat aaaaaatcat gtaatacagc ttggtacatg atttttattt gtagctgttt
      361 tttacataga cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt tactatctgt
      421 ttccagaagc tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc gctccgctag
      481 ttgctggctc actggaagaa gcaggaggag cagatgaaga cggtgtcgag ctttcttcgg
      541 atgaagaaga aggaaccgtt gttgaagacg ctggctcgct tgtttctgct gtcgtgcttt
      601 cttttgagga ctcagcagtt gattcgcttt gactagtaga gctagagctt tgagtcgttg
      661 tctctgttgt tgagcttcca ggagtctgga catagctgga agatggaata gttgttgatg
      721 gtgtaattgc attcgatcta gctgtatatt gatctttgta gtacagttca ccgcccacac
      781 gaattaatcc gcttggcatc tcccagtctg tatttgtcac gttagctgaa acatactgca
      841 taagctcgcg ataaacatca gaggctacgt gtgacgattc tgaagtgaca ggcgtcattt
      901 ttttattgta acctgtccac acggaaatcg aataatttgg cgtatagccc gcaaatagga
      961 tatctggata caccccagaa gaaataccaa gttttgcata ctcgtcatct gtgtaattgg
     1021 atgttccggt cttaccggct tgatataacc cagcgatttg cgcattcgta ccagttcctt
     1081 cagtgatcgt atcttttaaa atgtctgtga tcatataagc tgtttctgga gacatggctg
     1141 tttttccatc tggctcataa gtttcttctg ttccgtcttg gaagacgatt ttattaacat
     1201 attgaggttt gtaataagtc cctccattgg cgaaagccgc ataggctgct gccattttta
     1261 aggaagaagc tccgtatttt gtcccatctt gttcttccgt attactagag atcgcattcg
     1321 attgatggat cgtactgtat tcgatcccaa gattttttaa gaaagaagcc actttatctg
     1381 aaccgacttc attgaataat ttgactgctg gaacattacg ggataagtaa agtgcttggc
     1441 gtaaagtaat ggtgcccata tattgattat cccagtttcc tacaggagtc gaagtccctt
     1501 catagttata cggtgcgtca gtaatagttt ttccagtaga atatttcaaa tattcaaaag
     1561 ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa gtattcactg
     1621 ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc tgggctttta
     1681 ctttcccggt attggtgtcg ataagggtgg aggcaacttg catctcgtcg tctgggtaag
     1741 agacatactg atccgtgttg acgatatcat atagtttctt ttgggcgtcc agatccaaat
     1801 tcgtataaat ctctaaaccg tctgtgtaga cattcttatc tgttttttct tgtacttcat
     1861 tgatgacttc tttgacataa ttatcgacga tcttagtatt gtcatcggat tgagttagtt
     1921 cttgcagacc atccgttacc ggtacattaa cagcttgatc atattctgtt tgagagattt
     1981 tttcgttttg aagcatcgta tacaaaacag tatctcgtct tttcttcgcc tgatctggat
     2041 aaacataagg atcataggcg gaaggggctt gaggcatccc agcaagcaat gctgtttgcg
     2101 gtaaagaaag ttctgataat ttcttaccaa agtacatctc agaagctgtc tccatcccgt
     2161 acaagccgtt tgacatatac actttattta cgtagtaggt caagatctct tgtttcgatt
     2221 ttttttgttc caatcttaca gccatccacg cttcttgggc cttgcgtttc agagtctgat
     2281 cctcggcact ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa gtacttccgc
     2341 cttgcagtcc gccagaggta aagttagaga gggcagaacc gatgatccga atagggtcaa
     2401 ccccgatgtg tttataaaaa cggcgatctt ctaccgacac gatcgcatct tctaatgttt
     2461 tcggtaattc attcgcagaa attttttcac gcttttctgc gcctaaatct tcgaatagtt
     2521 cgccatcttg tgtatatagt ttggaagata cggtagcgtc taatttttta tccgtcaatt
     2581 cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag aggatacaga
     2641 aaaatgagag tattcctaaa aggatcttga taaataatcc ttttttatgt gttccagagg
     2701 actttccaga acctttgcca gaattcttct tcacactctt tttaggagta ggctgtttct
     2761 gtcgtctaga gcttctagtt tgttcatttg ccattcttgc tttctccttc aatagataat
     2821 gttgataaat agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat accagttttg
     2881 atctctattc ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc tttttccttt
     2941 tgggcgtccc agtaagcaat caaatgaaca ctattcaaat aaaaacagcg cttcaaagac
     3001 gaaaaccata acaagacaaa gcagacccca ttttgttcga ggcatgcttt catatgttcg
     3061 atttgatggg catggaagtt tttaaaggga aaagatgttt tgttcttggt ttcttttgct
     3121 tcaaagtcca agtaaaagcc tcgatagacg ccattatagt ctgtagttga ggcttgggta
     3181 aagtaggctt ccttgatcac agcagcactt ctcttaggat agtctacttt cacgatttgg
     3241 ataggggtag gctttttatg gataaccgcc aacccgcggg aaagataata tttattactt
     3301 tcattgattg cttcttcaaa gcgcatcccc cgattgccga attcagtgat ctgatttctt
     3361 ttttcttgaa ttttttcgac gggagaggca ttcttataat aaggttggcc atttggatag
     3421 cgcaatacca tctttaatca cactcctagg ccacattata gcaaaaaagc ggatagaaag
     3481 aaaaggaatc tgagatggat aagtcaaaag taatctatgt gtctgggtat cgaagtttcg
     3541 aactcggtgt ttttaaagaa aatgatccta aaatccaagt tgttaaaaat gttttaaaaa
     3601 aagaactcca tcagttagca gaagaagggc tggaatgggt acttgtcagt gggaatttgg
     3661 gcgtcgaact atgggctgtg caaatagtag cggaattgaa agaagaatat ccggaactat
     3721 ctcttggtat tttgtatcct ttttctgatt ttggtaataa ctggaatgaa gggaaccaag
     3781 aaaaaaaaca attagcagaa caattagcag attatgtcga agctgttagc catcaaccct
     3841 accaatcacc tagtcagcta aagaatcaca ctcgttttat acttgagcat acagtgggct
     3901 gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa gtattttttt gaagatgcac
     3961 agaattacca agcagatcat ccctacgaaa ttcggctgat cagtatggac gatcttcaaa
     4021 attttagtga ataatcgata ttgtgtttga tttatcaact tttttcctat acaatgaaaa
     4081 aggtagaaat gttattacgt aaatgaaaac tagaggtgta aatatggcga atttggttta
     4141 tagtcctaaa gacattttac aacaagaatt caaaacaaaa atgcgcggct atgatcccgt
     4201 tgaagttgat gaattcttag acaatatcat caaagattat gaaacttaca gcaaagaact
     4261 ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat tatctaaaac
     4321 tcaaggagca gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg cagcagtgac
     4381 caattttgat atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg gtaaaaagct
     4441 tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat ccaaataact acacaaatgc
     4501 agacacaagc ttagatgata atgaaaaaac acgtcaattt taatttttga ttaatgaatt
     4561 tgcggttttc gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc atgctcgcac
     4621 aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt actctttgga
     4681 gtaacggcag gaaaacaagc taaggcttcg gctatgctta agtatccttg aaagtgccac
     4741 agtgacgaag cggctgggga aacccatccg gtggaacgcg gtaaacccct cgagcgagca
     4801 acccaaacaa tggtaggggc gctttttgtc tggaagaaga acaggacaaa aaggcagtat
     4861 aactgcagat agatggttac cgctttgttt tttctccctg gaaaagcaaa gtacagaaca
     4921 tggcttatag aaaaccgcaa acatcaggga attaggtcgt gacaaaagta atgcgttata
     4981 agaaacaaga aaaacatttg aaagctattt ttcaatactt cagatgaatc gttcttatta
     5041 caaaacaaac ttttgtatca ccggttattc ggaatttgag taaaagtgtg gggtaggact
     5101 gatgtcccac ctcctttttt tataaataag gagattgtgt atggaaaaaa acaggaagtt
     5161 caacctcgtc gcaactgcgg caagcgggct agaagcactg gttggtaaag aattaagaga
     5221 tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa caatggaaac
     5281 gatcgctaca gctaatttat ggttacgtac agcagatcga gtaaaaatcg tggttgggga
     5341 attcgatgcg tatacgtttg atgaattgtt tgaaaacgta aaagcattgc cttgggaaga
     5401 ttttcttcca ctagatgcag cttttcctgt agctggaaaa tcgatcaagt cgaaactata
     5461 tagcacacct gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc tccgtaccta
     5521 ttatcaccgt ccagcttctg taccactgac ggaaactggc gcacattttc agttggaagt
     5581 agctttgctg aaagaccatg tgatggtaac tctggatacg acaggaccaa gtttattcaa
     5641 acgtggttat cgcttagaaa aaggtggagc accattgaaa gaaaacatgg cagcagcact
     5701 tattatgttg acaaattggc gaaaagaccg tccattttat gatcctgtat gtggttcagg
     5761 tacgctttgt attgaagcag ccttaatagg ccataatatc gcaccaggat tcaatcgtga
     5821 gtttgcttgc gaatcttggg attggttcag tcaagaaatc atggaaaatg ttcgatcagc
     5881 agccgaagaa aaagcagatt atgatattga attagatatt actggttcag atatcaatgg
     5941 acggatgatt gaaattgcca aagccaatgc agaagaaatc ggattaggct cttcgattac
     6001 ctttaagcaa caagcagtca aagacttcaa aacggataaa gaatacgggg tcatcgtggc
     6061 taatccgcct tacggagaac gtctaggaga agaagaaact gttcggaaat tatataaaga
     6121 aatgggcgat gtattccgtc cattaaaaac atggagtaaa tatatcttga ctagcgactt
     6181 agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt acaatggtgc
     6241 attgcgtacg gatctattcc agtattgggg aactcgtccg cctagaaaaa aagagaaaga
     6301 cgaataatct tactaattga ttcattcaat tggatgtttg gaggaatagg gaatgaaaga
     6361 aaaagagttt ttacaagaag taaaagaaat caatcttcta caacaagctt taggaatcct
     6421 cgattgggat acacaaacag gtatgcctga aaaagctagt gcataccgaa gtgaagtaga
     6481 cagctatctt tacagcattt atttttcaaa aaaaatcggt ccaaaaatca aagaagccat
     6541 tcgctatttt tctgaacatc cggaagaatt gtcggaagtt ggaactgccg tttttgccaa
     6601 agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa gatcttatga gaaaatatac
     6661 agctgccgtt tcttcagcac acggacaatg gcaaaaagct agagaaacac aacaattttc
     6721 tgattttcaa cacgcattga atcaaaatat tgaattaaca aaacagttga taccttactg
     6781 gagaaaagat gaaaagacga attatgatgt tttgctgaat caatacgaac ccgggatgac
     6841 tgtcgaaatc cttgataagg tatttgatca gcttaaagaa ggaattcttt cgatccgccg
     6901 tacgctccaa gaaaaaggga ctgaacctga aacagacttt ttaaatcgga gaatgacgaa
     6961 aaaacaacag aagagatttg tagtgaaagt aatcgaacaa cttggttatg attttagtcg
     7021 tggtcgttta gatgacactg tccacccatt tatgactgga attaatccga atgatgcacg
     7081 aatcacgaca cgatggaacg aaacagattt caaaatggct gtttttggag tgatccacga
     7141 ggctggtcat ggtatgtatg aacaagacat cgatgataaa tatgcgtata caccggttta
     7201 tgaaggtaca tcaatgggta tccatgaatc acaatcgttg tttaacgaga tcattattgg
     7261 aagtagtcgc agcttctgga aaaagcaata tcctttcttt caagaatgtg cagaaggaac
     7321 attcgatgat attccttttg acaaatttta tcgttcattg aaccatacaa aatcaagttt
     7381 ggttcgtatc gaggcagaca gtttgactta ccctctccat atcatcattc gttatgaaat
     7441 cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa ctacctgaaa tatggaatca
     7501 aaaatatgaa gagtatctgg gaatacgacc aacaaatgat gtggaaggca tcctacaaga
     7561 cgttcattgg tcaggtgcaa gctttggtta ttttccctct tatgcattag ggtacatgta
     7621 tgcggcacaa ttacttcatt ctttgcagca agaaatagat gtggaaaatg ttctagcgtc
     7681 agacgattat tctccaatca aaaactggat gtcagagcat atccatcaat atggtgcatc
     7741 tagaaaacca aatcaattaa tcatggatgc aacacatgaa ccgcttaatc cgcaatacct
     7801 gatcgactat atgaaagacc tgtattttaa tgtatatcag gtaaaataaa atacaattta
     7861 caaagatagc tgaaattcat ctaatctata gatgttttca gctatttact taagaaagaa
     7921 gtggaacgaa tggataaatt acaagtgatc gctgattata gtatggacca attagcgcat
     7981 gatcaaacag gacatgggag cgatcatacc aaaagagttg tcaagttagc tgaaaaaatt
     8041 ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg cagcagctta cctacatgat
     8101 acgattgatg ataaagtcgt taaaaatgag aacgaggcaa agcaacagct tcgtgtattc
     8161 cttcgctctt tgccaatagt agaggaacag atctcgatga ttttttccat tatcgaaaat
     8221 atgtcctttt ctaaaaatct ctctgaagcg gccgaactat ctctggaagg taagatcgtt
     8281 caagacgctg atcgtatcga agcattagga gcaatcggca ttttgcgtac tgcttatttc
     8341 ggcggtggac atgggcaccc gatttttgat cctgaactgt atccgcaaac attcaaggac
     8401 aaagaaaatt accgaaaagg cacgacagtg atcaatcatt tttatgaaaa actgttcttg
     8461 ctgtctgata aaatgaatac tgattacgga tatgaagaag ccaaacgaag agaaaccttc
     8521 atgcgtgaat tcttagaaga attttttctg gaatggtcta ttgacgatga aaattttact
     8581 ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa gtggtcagct
     8641 ccaagatatt tcggatcatt ttccgtaaaa gttacttctg gaacactgtt taccaagagg
     8701 tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat agcttctcgt
     8761 gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt ttatt
//
LOCUS       DASJTM010000107         8580 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJTM010000107 DASJTM010000000
VERSION     DASJTM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.108"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..807)
                     /locus_tag="U4B29_002480"
     CDS             complement(<1..807)
                     /locus_tag="U4B29_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2107384.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRII"
     gene            953..1756
                     /locus_tag="U4B29_002481"
     CDS             953..1756
                     /locus_tag="U4B29_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN2107385.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1934..2881
                     /locus_tag="U4B29_002482"
     CDS             1934..2881
                     /locus_tag="U4B29_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN2107386.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2949..3242)
                     /locus_tag="U4B29_002483"
     CDS             complement(2949..3242)
                     /locus_tag="U4B29_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN2107387.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3270..3935)
                     /locus_tag="U4B29_002484"
     CDS             complement(3270..3935)
                     /locus_tag="U4B29_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN2107388.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4075..4707)
                     /locus_tag="U4B29_002485"
     CDS             complement(4075..4707)
                     /locus_tag="U4B29_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2107389.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4714..5781)
                     /locus_tag="U4B29_002486"
     CDS             complement(4714..5781)
                     /locus_tag="U4B29_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2107390.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5778..6509)
                     /locus_tag="U4B29_002487"
     CDS             complement(5778..6509)
                     /locus_tag="U4B29_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN2107391.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6678..7157)
                     /gene="greA"
                     /locus_tag="U4B29_002488"
     CDS             complement(6678..7157)
                     /gene="greA"
                     /locus_tag="U4B29_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN2107392.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7293..8468)
                     /gene="mltG"
                     /locus_tag="U4B29_002489"
     CDS             complement(7293..8468)
                     /gene="mltG"
                     /locus_tag="U4B29_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN2107393.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2550 a         1765 c         1332 g         2933 t
ORIGIN      
        1 aattatacga ggatatatct tttcaatctc gcttagtagc tgatcttcaa aatcaatatg
       61 tccgtcgaaa ataattatct tttcgctata ctgccaaata tggacatgat gaacatcctt
      121 taccccatcg attgcttcaa tttcttgttc cattttttca tagtcgatag caggtgcact
      181 ttgtatcaaa atagcggcag cttttttgat caccattatc gcttctatca atatatatat
      241 cgaaatgatc agtgtaataa ctggatcgat ccagtagata tcgaaaaaac ggatcaaaat
      301 tgctccgata atcacactta ccgaagacag tgtatcactt aacaaatgca aataactaga
      361 ttttatattc aaactttcat ctgcttcttg tcgtaataat aatactgaga acaaattcgc
      421 gagcaatcca ataactgcta ctgttagcat caaatgacta tttatctttt ctggtgaagt
      481 gaaccgcttg aatgcttcaa cggttaaaaa caaggataat atgatcaata tgaatgagtt
      541 gatgaaagca ctcaatattt ctaaacgctg gtagccatac gtacgttttt tatttgcttc
      601 ttttcgggcc attttttgtg ccccgtaaga aaaaataatt gccaatgtat cactaaggtt
      661 atggaaagca tctgaaacaa gagacaaact ccctgacata atccctccaa taaattctga
      721 taaagtgatc accgcattca aaaccactac tccaaatagt ttttttgatg atctctgtgg
      781 ctgtgtaggc actattcctt cctccatttt atttatattg gatagtcttt agacaagaat
      841 aacatttttt tgatatttca ccaatcattc agctgtttcc ttaaagcact tgtatttcac
      901 ttgatacttc gctagaattg caagtgatga cactactaga tgaggagtac ttatgacaac
      961 aaaacaatta attatcagtt cctttatacg attcaaagac ctaaaggagg cgagaaatgt
     1021 cccattttgg aaagccattg tctatctttt agttttgagt ataattatgg ctttgccaat
     1081 ctcttttcaa gtttttcaag tcttaaacaa cattcaattg gatgggcaaa agattgctac
     1141 tcgcattcct gattttacaa tccaaaacgg gcagcttcaa accgaagaaa aagaaggatt
     1201 catttaccaa acaaactcca ttatcttcac atttgatcca gagggaaaac gaacagaaca
     1261 ggacatctct actgatttga tggggaattt tgttagtgtc gggatgttaa aaaacaaact
     1321 tatcatagct cttcctaata caggcacgac ttctgctttg ttgaacaata accaattaga
     1381 acttccctac accaatgaat ctttaaaaaa cttaacaggt aagcagctcc gttctttttt
     1441 aagtgaagca tctataccac tttggataaa agtcctgaca tttatatttt ctatttatcc
     1501 tagttttctg aatctgctga tcactttgct atttaccaac attgctgctt ttctctatgc
     1561 acgctttcga ctaacaaaag ctacttttct tgattgttta aaaacattga tctattcaat
     1621 ttcattgcct actataattg caacaatctt gatggtcttt cttccttctt ttgacactag
     1681 cgccttcatt gcttttgcag gaatctttat ttttgctcaa gctgtcaaag gatgggcaaa
     1741 aatatcgatc aattaagaaa taaagacaac tcgttatttc tacttcaata gaccctttta
     1801 gaggttgtga catttttttg tcacagcccc ttttttattt gaaaaatatt tctttctcaa
     1861 aaaacaagca aggcgcttca aagttcttct tgtatgcgct acacttagag taaagaaagg
     1921 cgggaaatta actatgtcag aaagaatcat ttatgcagat cgtccatttc gtgatgaatt
     1981 tatcgaagaa atccatacta tagcgcctgg ttatatcttt aaaacagaat tacaaccaga
     2041 ggatctacaa aatgtagaga tcagtttggg ctggaactca gactaccaag aaaaactatt
     2101 agcctcttct accttgaaat gggtccaatc tatctcagct ggtgttgatc agctacccct
     2161 tgagactttt tcaaagaaac atattttact ttcaaatggt agtggcatcc acagtgaatc
     2221 gattacggag catgtcttag gaatcatcct tgggtattct cgtggtcttt ttcaagcaca
     2281 aaaggcacag agtgacatga aatggttagg ttcatcggtc cattaccaat ctatccaagg
     2341 gaaaaaaata ctaatcattg gaactggcca tattggaaaa atgattgcaa aaaaatgcaa
     2401 agcattagac atgaactgct atggaatcaa tacatccggt catccagcag aaggaatgga
     2461 aaagactttt tctttaaatc aactaaagga agttcttcct gaagtagatt tcgtagtcaa
     2521 tatcttacct ctaacggagg aaacaactgg gctctttgat cagcatatgt ttgaacactt
     2581 tgatccaaaa tctgtattca tcaatgttgg ccgtggtgct tctgttaaga cacaagatct
     2641 ggtgcaggct ttaaataacc agcaactttc ctttgcagca ttagacgttt ttgaggaaga
     2701 gcctcttcca caagatcatc ctctttggaa aatggacaat gtactgatta cttcccacat
     2761 tgcaggtttg acacctgatt ttcaaaagaa attaatggct atttttttaa ccaatctgaa
     2821 aagctacttt gccacccatg agcttcaaac caatcaagta aaattgtctg caggatatta
     2881 atagctgaaa acaatatttt caaaagaaaa aagagaaggt gacaaaaatt gtgtcaccct
     2941 ctcttttttt attgtttttt aaagtgatag gttggctcct ccaggttggg atcataatca
     3001 acaagtaaat catatccttt aaagaaccat tcatctgctt cctcgataaa aaaaagtgtc
     3061 ccatcaattt ccactttctt tacaggatgt tctggctgtt caactgacat tccaatcgaa
     3121 aaaccatcat gaacctctgt tttgccgtag acttttccaa aaaaacgtat tccgtaagtt
     3181 tctggcagat caatttctga gtcaaaccac ttttgtgctt ttggcgttac cgttaatttc
     3241 atcccaacaa cctactttct cttttctttc tattgattca tcttatcgtc tagaatatcc
     3301 acatcgcctg tctctccaat aaccagtaga ttatcatttt cacgaacata ttcatctgct
     3361 gcaggagaag cgataaccgt ttgatctact cttcgtatgg cgactactgt taagtcaaaa
     3421 cgttgcctga aatttaattc agctaatgtt ttgttgaaaa atttaggatt ggagactcgg
     3481 atttctgcta aagaatattt atctgacaac tctaggtaat ctaaaatatt ctttgacact
     3541 aaattatgcg caatccttat tcccatgtct cgctcgggat ggacgacgcg atcagctccg
     3601 atcttttcaa gtactctggc atgatactca ttttgggctt ttgctaaaat atttggtacg
     3661 cccatttcct ttaccatcag agtaactaag atactggctt gaatatcttc tccaatagcg
     3721 acaattacat gatcgaaatt tcttatgcct aatgatcgaa gtgtcatttc atcctgtgca
     3781 tttgccacaa cagcatgagt ggctatattc atatattcat tgacccgatc ttcattacta
     3841 tcaatagcca gtacttcttg accagcttcg atcagtgttc ggcagatact tccgccaaat
     3901 cgtccaagac ctacaattgc aaaattttgt ttcaccgttt tcctccttta gtatcttctt
     3961 ttctcagtat aacaaagcct ttacaaaaac aaaagcaagc atcattattg taaaaaagcc
     4021 aagaaaaata atagttattt tctcagcttt tgattatcct ctttatcatc gtttttattt
     4081 caccaaacca tgtttgaatg catagatagc ggcttgtgta cggtcttcca cttctagctt
     4141 agccaatata ttggaaacat gtgtttttac tgttttcagt gtaataaaca attcgtccgc
     4201 tatttcctga ttactttttc cttcagaaat cagcattaaa atttcatttt cccgattcgt
     4261 tagttcttca tgtaatacag gctcatttcg ccgactcatt ttttccatca tttttgttgt
     4321 cacttctggt tctaatactc gctcgccacg ttgcgttgcc cttatagcat cagcaatttc
     4381 atgtgcggta gaggtcttca acaaataccc tgctgcacct gcttcaatgg caggataaac
     4441 tttttcgtca tcaataaaac tggttacgat aatgatttta gcttctggcc aatcttttaa
     4501 gattgctttt gttgattcga taccatccat ttcttccatg actaagtcca ttaaaatgac
     4561 gtccggtcga agtgtcatcg ccttctcata gccttgtcgt ccattttcag cttctccaat
     4621 tacttcaatg tcttcttgaa tagataaata aaaagagacg cctaagcgca ccatttcatg
     4681 gtcatctact aataaaactt ttatcatcct tatctatgcc tccttcatca aagggacctt
     4741 gatttctaca cttgtacctt gacctttaaa actaatgatt tttactgttc caccaatacc
     4801 agctactcgt tctttgatat tgttcaatcc ataacttccc gttttcgttt cattcatatt
     4861 aaaccctgtt ccgtcgtcaa taatacgtaa caaaagatta ttgtctattt tgtgcaaata
     4921 tacctctaat tcattggctt tagcatgtct taatgtattt gataacaatt cttgaacgat
     4981 tcggaacaag tgatcctcaa tggaactaga tagttttaca tcttctacat cccatttcag
     5041 cgaaatctgg attttatttt gcaattcctt caataactgc tcgatacctt gctttaaact
     5101 tttctcttct aaattgactg gacgtagatg aagaagcagc gcacgcatct cagactggga
     5161 agcgttgatg atctctgcta ccattttcaa ctgcttgcga aacatttctg gtgtctcgct
     5221 tttttctgct tgctccgtca atgccgacat catcatcatt gctgcaaaca attgttgcga
     5281 aactgaatca tgcagctcac gggctaatcg atgtctttct aattctaaaa tttcttcttt
     5341 ggtttgtcca tctacatatt ggggacggct cgttacaatt tgtaattcac tagatacttc
     5401 aatcattttt tcttttatct tagtgatttc cttatcaata tctttgatgt ataaatcgtc
     5461 cgatgcactt gggatcggaa gaagcaacaa tttggattca taattgccag aagaaagtgc
     5521 acgcagcttt tcttcgattt tcccgtattt tgtcttttga ataagtgata ataatagaaa
     5581 gacaattaat cctacaccta aggaaatggc taacaaatgg aagataagcg gaacatataa
     5641 taaacgtgca cgcagtaatt ctccccacca atgactttga ttgctggcat aaaaataagt
     5701 gaaaagtgaa aatagaataa taaggaaagc cgcaatcccc gaataaacag ctagcatcgc
     5761 tctggatatt tttcccatca tattcgtatc acctcgacat ctccaatcaa ggtattagta
     5821 ataatcttca atctacgagg gttttcatca taatcactgc tgtagatttt gatttgttca
     5881 tttttcaaag aaaattgctc ttcttcaaac aagacatttc ctactaatgt cgcatgctct
     5941 aatcggatgg ctacgcccaa aggtactaag atccgtgttc tgcctatccc ttttcttaca
     6001 atcacaatat tatcatcttt tggcaaaagc gtattgccca aatctatgat agtatcacca
     6061 gaaatgacgg cgatgttgat atcgtcccat tcatagacgt cgtttccaat acgttggtta
     6121 ccgaataatt gctgtctttt ccgctcatta ttatgtgttt ttaattgctc tgtttggatc
     6181 atcatgatct gtttctttcg ccaaaaagca tttttggtta aatctatccc tgaaatctct
     6241 acacctttca gaccaataaa taaaacggca aatacagcca tcatccataa agcaggactg
     6301 ttgactaagc taaaaaaaat gaccaaactt cctaatatca attgaaaatt ttgaaattta
     6361 gtacgaggat gtttgcgcat agctagatat atattgaaaa taccgaatat cactaaaagc
     6421 aaaagtccag tattgtttac gatttgccaa acagcaaata acagcagtag tgcttcaact
     6481 accacaaaaa aacgccaaga actattcatc gctctttctc cctcctgaca cattctcatt
     6541 tcatcatacc tattttttta actgttggct ataggacttt agatgtaaga tcgtttgttg
     6601 aaatctaaaa tacacaaaga gaaaaagatc tagagtgtat catcagtaag atgaactctc
     6661 tagatctttc agtcagacta cgcttgttct actttgacga ttttaactaa catatcgcca
     6721 cctggagtag caatagttac ttcatcattt ttacgtttgc caatcaatgc ttgcgcaata
     6781 ggagaatcat tggaaatttt gccagaaaac ggatctgctt cagcgcttcc gacaatcgtg
     6841 tactcttctt cttctccatc tggtagttcg atgaatgtaa ctgttttacc aattgatacc
     6901 tcatctgcat ctacaccgtc gttatcgatg atttgagcaa aacgaatcat attttcaatt
     6961 gttgtgattc ttccttcaac aaatgcttgt tcatctttcg ctgattcata ttcagagttt
     7021 tcggaaagat ctccgaaacc gcgggcaatt ttgattcgtt caatgatttc tccgcgtttg
     7081 actgttttta gttcttctaa ttctttttct aatttttctt taccttctag agtcattgga
     7141 taaactttct ctaccatgtg taacgtctcc ttttttcgat aaaataccgc aaaaggtaat
     7201 cttttgagga atatagtcca aaaaagatta ccacgctttt cttcaaatgt aaatgttttt
     7261 ctaaaaatga tcaatgtcta gagcctgttt ttctaagaat tatttacata tttttccact
     7321 aattccatat gttcttcata agttttagcg aaataaacat ttccagtgct cgtatctgct
     7381 acgaaataat aatagtcatt ttctgctggt tctaatactg ccttgattgc ctcttcgctt
     7441 ggattgtcaa aaggtccagg tccatagccg gtattggtat acaaattata tggtgaatct
     7501 acagctgtat cttcatacgt cactaattct ttgtgttcac ctaatgcgta taaaacagag
     7561 atatctgact gaagtggcat atttgctttg attcggttaa agaagacttg tgcaatgttt
     7621 ttacgatcat tttcttttac gccttctttt tcaactagag aagctagagt taaaacttct
     7681 tgtacagtca aatttttctg cttgatagct gaataatagt tttgcatgac cgtattcgtc
     7741 ttgttcacca tttcaatcac tagatcctta agcgaagtac tcgtgtaata atcatatgtg
     7801 gctgggaata agtaaccttc caaacggtat tttgtgttct cagctttaga tgcactttcc
     7861 aacagttctg ggaatttttg gtgcaactcg ttaaagaaag tctcatcatt cactaaatct
     7921 aaaaattctt tcttgtcttt accagttacc ttcgctaccc gttcagcaat ctgatcgata
     7981 tcgtaccctt ctggaatggc aattttccca tctgcaactt ttgttggctc agaagtaccg
     8041 ccttcttgca actgtttgcc aatctcgtca agagtcatat taggcgctaa ctgataatag
     8101 ccagcttgaa agccagttaa atttttaaat tttgtataat aattgaaaac aatcccgctt
     8161 ttaataatat tgtccttctc caaaatctca ccaatctgct tgttagaaga gccactcggg
     8221 atctcgactt ggactaattg gtcatctgat ttatctaaag gcttcaaccc gctatctaca
     8281 taacgataca cggtaaatcc aagaaatccg ccaataatca ataatgtaag agcaatcact
     8341 aaaatgatct tcctcacaat cttatcttct tttttacgca gatttctccg cttgtcgatt
     8401 atatctgctg tagctgcctt actggcttct tttttatctt tacttgggtg ttgtttgttt
     8461 cctgacaaaa caactcctcc ttactatcaa gttcgtaact ttattcatta tacatgatat
     8521 ttgaattggc gtgaatgatt ttatttaaat attctatcaa aaatgtaagt agtaaatcca
//
LOCUS       DASJTM010000108         8568 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJTM010000108 DASJTM010000000
VERSION     DASJTM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.109"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..401
                     /locus_tag="U4B29_002490"
     CDS             <1..401
                     /locus_tag="U4B29_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2107394.1"
                     /translation="PIRITNDLKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVS
                     QQIPTLEKMNLISISQKKGNKKTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFS
                     PDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            494..1656
                     /locus_tag="U4B29_002491"
     CDS             join(494..770,770..1656)
                     /locus_tag="U4B29_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2107395.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1936..2916
                     /locus_tag="U4B29_002492"
     CDS             1936..2916
                     /locus_tag="U4B29_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2107396.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3038..4456
                     /locus_tag="U4B29_002493"
     CDS             3038..4456
                     /locus_tag="U4B29_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN2107397.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4527..5996
                     /locus_tag="U4B29_002494"
     CDS             4527..5996
                     /locus_tag="U4B29_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2107398.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6006..6473
                     /locus_tag="U4B29_002495"
     CDS             6006..6473
                     /locus_tag="U4B29_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2107399.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6509..7381
                     /locus_tag="U4B29_002496"
     CDS             6509..7381
                     /locus_tag="U4B29_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2107400.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7463..>8568
                     /locus_tag="U4B29_002497"
     CDS             7463..>8568
                     /locus_tag="U4B29_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2107401.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEES"
BASE COUNT         2950 a         1290 c         1739 g         2589 t
ORIGIN      
        1 gaccaatcag aataactaat gatctaaaac tcagtacgag tatgattcat ttgattgatg
       61 tgattggaaa ttatcctgaa actagtatta cggaattagc tgatcgatta ggcgttacca
      121 aaggagctgt atcccaacag attcccactt tagagaaaat gaatcttata agcatttctc
      181 aaaaaaaagg aaataagaaa actaaacttg tatcgttaac aaaatatggt cgaaaggtgt
      241 acgaatcaca taattattta catgaagagc tttatagctc aataaatgaa gcgttagaat
      301 cattctcacc agaccaaatt tcaaccatta aacagattct agaaaaagta tcttatacaa
      361 tcaatgaata tcaagtaagt ctgacaagaa aggaagattg acatgaaaaa aatgattttt
      421 gaatcggcta cactagacta gacagaaaaa ataaggtgtg taaaataaat gaaaacacac
      481 tggaggaatt tttatgtcaa gacgtcaacg tagaacctat tcaaaagaat tcaaacaaca
      541 agtcgtcgat ctttatctcg caggaaagcc ccgagctgaa atcattcgag attatgagct
      601 gactacctcg gcctttgata agtgggtcaa gcaagcgcaa actactggct cgtttcagga
      661 aaaagataac ttgacccctg aacaagtaga actaattgca ttacgaaaga aaaataaaca
      721 gcttgaaatg gagaatgaca ttttaaagca agcggcgctg atattgggac gaaaagacaa
      781 gtaattgata ccaataagca caaatactca atatcagcga tgtgtaaagt gttaaacatc
      841 tcttgccaaa cctattatta tcaagccaaa ccagttgaag atgaatcaga actggaagaa
      901 ctcgttcaag aagaatttat tcgcaatcga aaagcttatg gtaccaggaa gctgaagcag
      961 tgcttggcca atctaggtct tcaagtgagt caacgcagaa tcggccgtat gatgaaacag
     1021 cgtggcttgc gttcaacata cacattggct catttcaaat tccattcttc tacttgtaac
     1081 gaagccaaga ccgccaatat tctagacgga accttcctac aggaaaagtc attagaagcg
     1141 attgtaaccg accttaccta tgttcgtgtt gggaagaaat ggcagtatat ctgcctgatt
     1201 cttgatttgt ttaatcgaga aatcatcggc tattcatgcg gggagaagaa agatgcgacg
     1261 ttagtcaagg aagcttttgc aaagattccg tactcgttga ataatgtaca actttttcac
     1321 acggatcgtg gaaaagaatt cgataaccag acgattgacg aaattttatg ggcctttaat
     1381 atcacgcgtt ccttaagcaa aaaaggctgc ccctatgata acgccgttgc agaatccacc
     1441 tataaatctg tcaaagtaga attcgttcat caataccgct ttgagacatt ggctaatttg
     1501 cgattagagc tatttgatta cgtccattgg tggaatacca tccggctgca cggcacgtta
     1561 ggctatgaga caccgatcag ttttcgacag cagagattgg cgcagcgaat ccttgataat
     1621 gagctcggat gtgataccgg tggcgaggca ccttaattat ttgaatgagc ttctgcctta
     1681 gagaatcaga ttcgaggagg ctcgttgtca agggcagtcg gaacaccagc agcaagctta
     1741 cgcaatacct tataattttt gtcaaaaaaa gtgttgccat tccacatata tctttaaaca
     1801 gctattgcca gataaaaata aatgatatta tatatataaa tacttatccg aaaaggggaa
     1861 tgagagagtc aaggttcttg gaatgtcgaa tagtcgagta ggtagatttt ctatctacaa
     1921 ccttgttgtt taaaaatgaa aaaaattgga attagagaag ttgcgaaaga ggcaaatgtt
     1981 tctcctacga ctgtgtcgag ggtattgaat gatcgtggat atttgagtga ggaaacaaaa
     2041 agaaaagttt ttgatgccat gaaaaaatta gaatactatc ctaatgaact ggcgagagca
     2101 ctttttaaaa acgaaacata taccgtaggt ttgatttttc ctacaattac taatccattt
     2161 cacgctgagt taattcaaga tatcgagttc gagctttcca gcaggggata taaagtgttg
     2221 ctgtgcaata gcttaaacaa tccaacaaaa gaaaaagagt acttgacgat gttgagaaag
     2281 aatcaggttg atggaattat agtaggaacg cacaataaag atattgcgga ttatgatatt
     2341 ccagggctac ctattgtagc gattgataga agtcttggtg aaaatacagt tacagtatct
     2401 tgtgataatt acaggggtgg ggaattagct actcaactac ttgtaaatca tggctgtcag
     2461 aatatactgt gtattcgggg agatagtaag ataaagctac ccgcgaatag taggacttta
     2521 gcatataaag atgttttgaa taaattcatg ctggaaccgt tagtcctaga agtaccattt
     2581 gtaaaagaaa tcaatgaaaa aaatgaaata attaagacat atctggaaac tcatccaaat
     2641 attgacgggg tttttgcagg agatgattta ttggcttctt tggcgattaa caacttagga
     2701 agttcgggca aaagagttcc aaaagaggtt aaagtgattg gatttgatgg agctcaacaa
     2761 acattgattt ataatccaga attatcaact attcaacagc ctatagatca aatatctaaa
     2821 gtagcagtaa aaaaattatt gaatatgatt aagggcgaaa aggagacgga tgaactttca
     2881 cttccagtaa agctcataaa aaaaaataca gcataagtta aatcggcgaa aaattccttc
     2941 gtcgatttaa aaatattttt atgaaacggg ttgacatatg aaacgggttg acatatatta
     3001 gttgtgaaga taaaaacctt ttcactggag gaagaaaatg gagaaattat taattaaaag
     3061 caataatcat caactttact accaacctaa ggatgtttgg gtcggagatg ttatgcctta
     3121 cgggaaagaa ggaaaattct ttatttatca tcaacgtgat actagaaatc cagttccttt
     3181 tggagaaccg tttggctggg ctttagcaac tacgaaagat tttgttcatt ttgaagactt
     3241 tggggaatct cttttaagag gggatgacaa tgagagagac cagtttatct acgctggttc
     3301 tgtatttgag gcaaatggaa aggttcacgc attttataca gggtataatc gtgaattctt
     3361 agcgcaggga aagacatctc aagtgttgct tcatgctgag agtgatgatt ttgttcattg
     3421 ggaaaaatca gaaacagcat tggagttaga accacaagaa gggtatgata ttcgaaactg
     3481 gagagatcca tttgtcgttt gggatgatga aaaagaagaa tatttgctaa ttttaggggc
     3541 gcgaaagggt caggataaac atctacaaac cggacgttta gtggaattta catcaaaaga
     3601 tttaactaat tgggaattca aaggtgattt ttgggctccg ggattgtata ccatgtttga
     3661 aatgccagaa cttttcaaga ttggtgactg gtggtatctt gtctattcag aatactcaga
     3721 tggaaataaa atactctatc gaatgagtca aagtttggaa ggtccttgga ttaagcctaa
     3781 agatgaagct tttgatggac gggcttatta tgcaggtaga acagcttatg atggaaaacg
     3841 tcgagtccta tttggatggg taccaacaaa agatcaagaa aatgataaaa acaattattt
     3901 atggggaggt actttggttc ctcatgaagt ctatcaaaaa gaggattttt cacttggggt
     3961 aaaacccata gacgaagtgt ggaatgcatt cataaattta catgatattg ccgatgtaga
     4021 gatagaaaca gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt
     4081 taatttagaa acaacaattt catttgatga gaatcttagt gacttttcac tgagattata
     4141 taaaaacctt gagacagatg aatcttacga atatcgcttc aatttagatg aggggcgtct
     4201 tacatttgat aaaaaccctt gttacccttg gtttcaatat atggataaag gacttattag
     4261 acctatatct ttaaagccta accaatctta taaactaaaa gtgattgttg atgacaatat
     4321 aatgacactt tatattgatg gaatcgctct taacgctcgt gtacatggtt gttttggtca
     4381 agcaattact attacagcaa caaatggatc aatgactgca ataaatacca agttttcaaa
     4441 tgaaataaag aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg
     4501 tcacatttaa aagggaggat aaatgaatgg actacaaaaa aattgccgaa gatgtattgg
     4561 aaaacgttgg aggaaaagag aatatctcaa gcgctacaca ctgcattact cgtttacgtt
     4621 taattcttaa agacaaaagc aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa
     4681 aaggtgttct tttcaatagt ggacagttac aagtaatttt cggaacaggt ataattgaaa
     4741 aggtttacaa agagttcgtt gaattgacag gagctaagga agtatccgtt tctgaaatga
     4801 aagacgaagg agtcaataat tcaggaaata gacttcaacg agcttttaaa gttttctcgg
     4861 atatctttat tccgatcatt ccagctttta ttggggcagc aatggtttta ggaattcgtt
     4921 cgttgattac cactcctgga atgtttggaa tggatggctc attcgctgat cacaatgtat
     4981 tcctaaatga tttatccaaa tttctgggta ttattgcaac cacatttgat tatttaccag
     5041 ttttagttat gtattctgca acaaaacgtt ttggcggaaa ccctatttta ggattacttg
     5101 taggatttgt tatgattcat ccaggactag ccaatcggaa tgatttcgtc atgggaacta
     5161 taaagcctga ttattggcat ttatttggat taagcattcc agcagttgcg tttcaagggg
     5221 gtgtttttcc agcaattttg acttcttggt ttttagcaaa agttgagaaa attacaaata
     5281 aatattctcc tcaagtactt gcttacattt tagtcccaac tgtgacaatt gttcttgcca
     5341 actttgcact atttttagtg tttggtccat tggggaattt tatcggtaat gttttaggtg
     5401 gaatcattga tttcctatat atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg
     5461 cgttacaacc attagttgta acaggaacac aacatgctat tcaaggtatt gaggcaaact
     5521 tagtcgcaac aactggattc aattatatcc aacctatttg gtcagtttca attattgccc
     5581 aaggtggcgg ttgtattggt atgtatttac tctttaagaa aaaatctaaa gatagagata
     5641 ttgcgatttc aagctttatt ccaacactgg taggtattac tgaaccggct atctttgctg
     5701 ttaatttgaa atattcaatc gttccttttg tttgctcgtt tattggtgct ggttttggtg
     5761 gagcatttat gaaaattttt gatgtaaaag ccattggaca aggattgact gtattgccag
     5821 gtctgactat tgctaatcca gttcttcctt atatatttgg taatctcata gcattcattt
     5881 taccaatcat ttttattctc gcttatgata aatataaagt gtttatccct gaagctaaac
     5941 accgtaaatt tgcagaaaaa actgactcaa aaaaattgcc agttcaagta aagtgagatg
     6001 agagcatgtt aaattttttt agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg
     6061 tttttgaaaa actggagaaa ttaagtgatc cagtcttttc aaaaggaatg atgggacaag
     6121 gatttgcaat tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa
     6181 gtatttttcc tacaaaacat gccttaggaa taaaaacaaa atcaggaatt gagatacttg
     6241 ttcatgtagg aatagataca gttgagctaa atggtgaggg atttgatatt aaagttcaag
     6301 aaggacaaaa agttaaggct ggggatattc ttatgaccat taattttgta gtttttgaaa
     6361 agaatcacaa gactaaagat gtcattgttg tttttccaga ctaccaagac gaaattttca
     6421 ttccgaaact tgaagaagta aaacaaggac aagaattgtt tgttcttggc taatgataag
     6481 taattaataa tagaatcgag gaaaatgtat ggtttttggg gctattgaag ctggtggaac
     6541 aaaatttgtt tgtgccattg gagataaaga tttgaacata tttgaaaaag tcagctttcc
     6601 cacgactaca cccaaagaaa caatgaattt agttttagat ttttttaatc atcataaaga
     6661 tgatttagaa ggtatcggag tgggatcgtt cggtcctatt gatattcatt gtgaatctaa
     6721 aacgtatggc ttcattacat caacgccaaa acttgcttgg caggattttg attttattgg
     6781 aagtttaaaa aagcaactta atattccaac tgcttggacg actgatgtaa atgcctcttg
     6841 ctatggtgaa tatgtttctg gaaatggcag aggcctttca agtgttgttt actataccgt
     6901 aggaacgggc ataggaggtg gcgctatcca aaacggaaac ttcattgaag gttttagtca
     6961 tccagaaatg ggacatatgc ttgtaaaacg acacaataaa gacaactttt ctgggacatg
     7021 tccattccac ggagactgtt tagagggctt agctgctggt ccggcaatcg aaagaagatc
     7081 agggaaaaag ggacaagaag tgactgaaaa tgacgttact tgggaaatag aagcttatta
     7141 cattgctcag tgtgcatata atacaacatt gatgttatca cctgatgtca ttatatttgg
     7201 tggcggagta atgaaacaaa aacacttaaa aaagaaagta caagataagt ttattgagtt
     7261 gatcaacgga tatgtcgcta ttcccgatgt tgacaattat attatgacac caaaattgtt
     7321 agataattct ggtattgtag gttgtttagc tttagcaaga gacgttaaag aaaaatctta
     7381 atttggaaaa aagatcgtct cgactatttg tttggttgat gcgatctttt ttttcaatta
     7441 gtaattgaag ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat tagagtatat
     7501 caaaaataat ttaaagtatt acgatttttc tgctcaagat attgcaatga aattttgtat
     7561 taaaagaaat gtcgcttccc actatttgaa tcagttattt tcagatggaa aattactaaa
     7621 gaatgattct gttagaccag taatgttcaa gtataatcaa caaaagccaa aagactgctt
     7681 ttctaagttt attggagcag acatatcttt gaaatccact atagataaat gcaaagctac
     7741 tgtaatgtat ccccctaatg gcctcccatt aataataaaa ggaaacagtg gcgttggaaa
     7801 aagtttttta gcaagtttga tctatcagta tgctcttgat agaaaagtaa ttcataatga
     7861 cgcaaaattt gttgttgtga attgtgcaga ttatgcaaat aatccagagc tattgtcagc
     7921 agttttattt ggatataaaa aaggagcctt tactggggct gaaaaagata ctgcagggat
     7981 gctatcttct gctaatggag gatacctttt tttagatgag gttcacaatc taagtgcaga
     8041 aaaccaagag aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac ttggtgaaag
     8101 tgtgcaatgg gagtatgcaa atgttcgcct tatcttagcc acaacagaag ataaaaatac
     8161 ctctttgtta gcaactttta gaagacgcat cccagctgaa atcactctgc cagattacaa
     8221 taatagaagt acatctgaaa aaattcaatt attatttgaa tttcttaaag atgaagcaat
     8281 tacaattgat tcaaaaatat tttgttcgat tgaacttttt actgatcttt tatcgaaaac
     8341 atttgaagga aatgttggag aattgaggaa tgaaatcaag cttctttgtg cagaaggtta
     8401 tttaggaaat aagagaaagt cttcaattta tttaggggac aaactagaca gcggtttttg
     8461 gatagatcca gaaattagta tagatttaaa aaaaatgttt cttaaatcac tttctgaaat
     8521 tgatttgatt gaactttttc aaaatcacat ttcaattgaa gaaagcat
//
LOCUS       DASJTM010000109         8494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJTM010000109 DASJTM010000000
VERSION     DASJTM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.110"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U4B29_002498"
     CDS             complement(<1..173)
                     /locus_tag="U4B29_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2107402.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U4B29_002499"
     CDS             complement(593..1714)
                     /locus_tag="U4B29_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN2107403.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U4B29_002500"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U4B29_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN2107404.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U4B29_002501"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U4B29_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN2107405.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U4B29_002502"
     CDS             complement(4304..5215)
                     /locus_tag="U4B29_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2107406.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U4B29_002503"
     CDS             complement(5232..6239)
                     /locus_tag="U4B29_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2107407.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U4B29_002504"
     CDS             complement(6236..8365)
                     /locus_tag="U4B29_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2107408.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="U4B29_002505"
     CDS             complement(8370..>8494)
                     /locus_tag="U4B29_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2107409.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DASJTM010000110         8468 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJTM010000110 DASJTM010000000
VERSION     DASJTM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.111"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(153..650)
                     /locus_tag="U4B29_002506"
     CDS             complement(153..650)
                     /locus_tag="U4B29_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN2107410.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(780..1490)
                     /gene="budA"
                     /locus_tag="U4B29_002507"
     CDS             complement(780..1490)
                     /gene="budA"
                     /locus_tag="U4B29_002507"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN2107411.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1503..3167)
                     /gene="alsS"
                     /locus_tag="U4B29_002508"
     CDS             complement(1503..3167)
                     /gene="alsS"
                     /locus_tag="U4B29_002508"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN2107412.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3372..4034)
                     /locus_tag="U4B29_002509"
     CDS             complement(3372..4034)
                     /locus_tag="U4B29_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2107413.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4044..4859)
                     /locus_tag="U4B29_002510"
     CDS             complement(4044..4859)
                     /locus_tag="U4B29_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2107414.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5121..5564)
                     /locus_tag="U4B29_002511"
     CDS             complement(5121..5564)
                     /locus_tag="U4B29_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN2107415.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5698..6036)
                     /locus_tag="U4B29_002512"
     CDS             complement(5698..6036)
                     /locus_tag="U4B29_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2107416.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(6024..6401)
                     /locus_tag="U4B29_002513"
     CDS             complement(6024..6401)
                     /locus_tag="U4B29_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2107417.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6625..7818
                     /locus_tag="U4B29_002514"
     CDS             6625..7818
                     /locus_tag="U4B29_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2107418.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7984..8406)
                     /locus_tag="U4B29_002515"
     CDS             complement(7984..8406)
                     /locus_tag="U4B29_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107419.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2503 a         1705 c         1421 g         2839 t
ORIGIN      
        1 taaaatgata ataccattaa aatatgatta tgaaaatcaa taacacttgt gattttataa
       61 aaaaataaaa aaaccgaaaa acagaaatgc ccactgtctt tccggctctt tttattcttg
      121 atttctcttc ttttttgttt caatagacga ttttagttat taataaatga aaaagcattt
      181 atattaaaat ttgctaataa aagatctaca tatttttcta gaaatgcttg atcgatttca
      241 tcataccctt tcgttactcc gcaatcgata tctaaaacac cgattaaatt accttctttt
      301 accattggca ctacgatttc tgattgtgcg gctgaatcgc aggaaatgta atttgcatgg
      361 gttttgacat catcaacgat caatgtttct tgtttttcag ctgattctcc acaaactcct
      421 tttcccatct tgatacgtgt gcaagaaact cgcccttgga acggtcctaa aatcaattca
      481 ttaccatcga ataaataata tcctgcaaag acagtctcag gcaaagtctc attcaacaat
      541 gcagatgaat ttgctaaatt tgctaaccag ttatcttctg cttctaataa tccttgttgc
      601 tgtaataata aaatttcgta tgcatctttt ttctgttttt ctgtccacat gataaaaacc
      661 gctccttttt gatctatgga gaagattata gctcagtttt tttgtaaacg ctaggtttcc
      721 tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa cgcatctcgc tgtctttttc
      781 tattctgctt gttggatttc tgctgctaaa tcagcataat caatcgtttc gtgaacaaaa
      841 gtcggatcat caactggaaa atgctgtaca agggacgaga tgcggctaat ctcaactttt
      901 ccttcagcca cttcaaaatc cattacatgt ccgccaaatg tatgtgtatc atcgataaaa
      961 tgcaagtgaa atcccgctgc agcaactcct tggaataatt caggcgtaaa gaacccgact
     1021 aatgttcctt ggacattgct ttgttggaat tctggttgag actctgaaat ttttgctagt
     1081 cttctgtaag gtttttcttg cttcggcatg atacggatgt gcatattttt gaataatcca
     1141 gttatcttca ctgccaaaaa taggttttcc ccggcttctc ttactaatat gtcttttttc
     1201 aattgttctc catctgcagt ttttgagaca gaaaaactag tatctggcgt aaaatctgta
     1261 attgctgcat agggggtaag ttcacttcca tctaattggg caactgagcc atctgatctt
     1321 ccttgataag ctacgccatc taaaaagatg acttcaccat caagcccatg caatgttccc
     1381 aatcccagag ttcctttttc taatatatga gcaatcgttt ctgttccatc catcaagcca
     1441 gccatcaaag ctcctaatgt cccgtgctgg tataagattt tttcagtcat gtcgacactt
     1501 ccttaataga attgatctgg taagattgtt tctcccaatt tctcattatc acggtaatca
     1561 atcggaatat caataattac gggtccttct gtcgcaaatc cttcttctaa agctttttct
     1621 aattcctcta cacttgtagc tcggattcct ttagcgccaa aagcttctgc atatttcaca
     1681 aaatcaactg gaccaaagtc tacacctgac gcccgctgat atttcatttt ttcttggaac
     1741 tctaccatat tgtagtgtcc atcattccaa atcaaatgaa cgatattcaa ttttttacgt
     1801 accgccgttt ctaaatcctg ggcagagaat aaaaatcccc catcgccgga aactgatacg
     1861 atttgtgtat ttggacgcac caatgcagca gaaatcgccc atggcaatgc aacaccaagc
     1921 gtttgcatac cattactgaa taacaaatga cgaggttcat aacttctaaa gtggcgtgcc
     1981 atccaaatat aatgactacc tacatctacg gtaacagtca tatcatctgt tacttttgac
     2041 tgaagcgtgt taataacttc taaaggatgc aagatacctg cttctccttt tgattgaaca
     2101 atgtcccgct ccgttaattc tgcttgaaga gatgctaaat attcttttgc atcttctgac
     2161 acttgttgag gctctaatga acctgtcaat aaatcaagag tggctgagat atcaccgatc
     2221 aattctcttt caggctgcat aaaaggatcg atttcagctg gcgcttcatc gatcacaatg
     2281 atccgcgcat ctttttccgc attccagttt cttgcttcat attcgattgg atcatagcca
     2341 atagcaataa ctagatcact tcgtttcaaa agcatatctc ctggttggtt tctaaataaa
     2401 cccacacgac caaagaaatg cgcttctaat tcacgtgaga tcactccagc tgcttggaat
     2461 gtctctacaa caggcaatgc tgtctttcca actaattgcc tgatggctaa cgtttctttt
     2521 tcacttgatc ctcgcatacc tactaatagt accggtagtt ttgcttctct aatgcgttca
     2581 gctaggtaac ggatatcatc tgcagaagca gaacctaatt taggatcgct caatggcttg
     2641 atgacattcc cttggacagg tgcatccact acatcttgcg ggatactgat aaagctcgcg
     2701 cctttttttg aactcttagc catacgataa gcattggcaa tcacttctga aattgtttct
     2761 ggatcttgga tttctgcact gtattttgtg attggctgga acagtgctgc attatccatg
     2821 ctttgatggg tcaacttcaa tagatcgctt cgttttactt gtccggcaat tgctaaaact
     2881 gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta agttactagc accaggacca
     2941 cttgttgcga taactactcc tggctctcct gtaatacgtc cgattgcttg tgccataaat
     3001 gcagcatttt gttcgtgacg agtcacgatt aattcaggac cttgatcctc taattcatta
     3061 aatacaccat cgatttttgc acctggtata ccgaatacat aatcaacatg atgattaatc
     3121 aaactttcta ctacagattt cgaaccttgt ttcatttttt cgtccattga acatcgtcct
     3181 ttttatgctt tatttttaat tattagttag aaataatcac aaataattga tacagtttct
     3241 aaacctgttt cattcaaaag tatagcacgt tttatttcat ttgtttagcc atatgtttca
     3301 aaaaatgaaa aagaatgaca gtttttacat aaaaactgtc attcttaatt gaaagtttca
     3361 atttataaag tctacagatc catagttctc atcaatttac gattgttgaa gtaaatcaaa
     3421 ccaaatacga tagaaggacc tagtagaata caccattggg gctttacgaa aaagaagaga
     3481 aacaaacttg tcaatagtaa aaaatctagt aaactcacat accatttttt caacgtatag
     3541 tagaacgcta tccgtagaac ttgatttggt ttcgacgctg gatttctcac ctgaaaatac
     3601 cagtagttga tgctaaagga aactgataaa caagcaagta aaacaaacaa cggaatcaac
     3661 cattttccat aagaaaaacg tataaaaaag aaacaatcaa aaatcattat cacactaact
     3721 atccaagcaa atagccaata aaaaaatccc tttagccaaa attggcgaaa cgtctttatg
     3781 aacaaacgta ctggtgaaaa acttttttct tttgctaatt cttctgtcat acctatcatc
     3841 acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa aggaaaagac attccttcca
     3901 tctatcgaca aaaggaacgc agctgctata tacaagatat tgacacacca aaaacaaagg
     3961 ctacttgcca atgattgata caaccaataa aagaattcca tataaacatt tttgtcaaaa
     4021 cgctgctctt tcataatcac ctcctattcc ttcattcctg atgaagcgat cccttctaca
     4081 aaatattttt gcatcgctaa aaatataaaa gcaacaggaa gaacagaaac aactgaggca
     4141 gccatgatta atgcgtactc tgcatcaaat agcccaataa acattttcaa ccctaactgg
     4201 attgttttat tttccgtaga agacaaataa atgaatggcc ccatataatc gttccatgta
     4261 ttaacgaatg tgatgatcgt caaactggca atcgccggtt ttgtaagagg taaaatgatc
     4321 ttgcgataga ttccccactc tgataagcca tcgatccgcg cactctcaca taaagaatct
     4381 gggatactac tgtagtattg cttcaacaag aacacaccaa aagcattgaa cgcttgtaat
     4441 agcaccaacg accacaacgt atatgttaaa cccacttttc tcatcataat gaactgcggg
     4501 atcatataag actgccaagg gacagcaatc gtgccgatat atgctaaaaa taaaatatcg
     4561 cgccctttaa aatttagttt agaaaacccg taagcagcaa aactagaagt aaacaactga
     4621 atcaacgtga tcacaacgct taaaaaagct gtattcttaa aaaatgtcac taaaggaata
     4681 cgagaccaaa taaccagata attctctaaa tgccaagttt tagggatcca ttgaatcgga
     4741 atcgtgaaca cttcattatt tgctttgaat gaagcagaaa ccatccataa aaaaggaatg
     4801 atcgtcaata aagctaatac aatcagcaga atcataccca accattgaat aaagggcatg
     4861 ttttttgaac gaatttttgc tttttttctt tcatttcgaa cacctccaaa gttataatct
     4921 tcatttcttt gtaatgatta caatccagaa caacggttat tatagctgat tcatttccgt
     4981 tcttcttatg tttgtaagcg cttatttaac aagttatgta aatctcttta ttttttttga
     5041 tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt ctcgcttttg tattgaaatt
     5101 tcttcgatta gagatactta ttactgttca aaagcttctt tgaaacggtg aatcaattca
     5161 ttgactacag gtgataaaat ccgattcttt ttccagacta gcatgcagtt cgtttcaatc
     5221 attgggtcaa gcggaacgaa tgtcactcgc tttgaaagat gacttacagc accttctatg
     5281 actaaagcag cacctacctg attctcaatt agcgcaaata cattgaaaat caaattgtac
     5341 gttcctgtga tcagcaactg ctcctttttt atgcctaacc agtcagcaaa cattcgctgt
     5401 atttctggac gctttgagat caaaagaggt acattcgcaa agtcttcttt tgttatcacg
     5461 tctttttttg ctaaaacaga attttctaaa atgagcaatc cccatttttc ctttcttggt
     5521 aacaaaatcg tttcgtattt ctctgtatct actggatcta gcaaaatcgc taaatctaac
     5581 aggctttttt ctagtcactc cgtgatatca tcacttgttc cgctgtggat atgaaattga
     5641 acgctgggat aatcacggat caacttttct agtaatagtg ccaatgtatt cgaattatca
     5701 gcttccacac agccgatcgt aaatcggccg ctgaataatt gtttttttct gttctcgaat
     5761 tcttgagtgg tttgattcgt taaatctaaa atttcttctg ctcggctttt ttagaaaata
     5821 acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca aacagttctg ttcctagttc
     5881 ctcttctaac tccttcagtt gtcggcttaa ggttggttgt gtaagatgta gaacttctgc
     5941 ggcttgagag attgttcctt cttctgcaat tgtccaaaaa tattttagta agcgtaactc
     6001 catagcgatg ctccttgatt tttttacttg atacattcca ttttatttct agataacaca
     6061 cgatcatatt tcttgatttt tccttgtaaa cgttccaaac actcttgcat ttcagcaatt
     6121 tctttcgtca aaagttcact ttcttcaatc aaaagcttac gccgctcaaa tacagttgct
     6181 tctccttcgc tagataggcg aatatattca atcaaagact cgatcgataa tccggcattt
     6241 cgcatacaga tcgtaaagtc gacccatttt aggtcttctt ctgtataatc acggattccg
     6301 ccttttgtcc gattaactgg tggaatcaac ccgattcgtt cataatagcg caatgtatcg
     6361 gccgaaatgc ccttttcttc actgacttgt ttgatattca tctgatcact ctttctctta
     6421 atttcttgcc actttactta tgtattttat ttattctaca actttaagtg tactccaagt
     6481 caatactcat ttctattaaa actaagctgg attatctata tcggttgttt gtgtattatt
     6541 tcaaataaaa aagtcttgtt tttttattct tttttggata tacttttaac aatttaggga
     6601 aattttagga aaaggggtat aaaaatgaca acgtatcagc gaaaggtatt attatcgaca
     6661 tcggcgggaa ttgctttaga gaatatggat attatgtttt tagctttttc attatcttca
     6721 atgattactt cttttcatat tagtggtacg caagcgggtt tcatttcaac gatcactaat
     6781 ttggggatgc tcattggagg tatttttttt ggcatcatag ctgataaata tggacgtgtg
     6841 aaagtatttt ctcaaacggt ccttctgttt tcgattgctt ctttattgat gtattttgct
     6901 tcaaacatct acttggttta tctctttcgc tttatcgcag gtgtaggagc gggaggagaa
     6961 tacggggcgt gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat tggacgagct
     7021 tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat tttagctgct
     7081 gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg taggtgtact gccggtgttg
     7141 atggtcttgt tcattcgtcg cggcttgact gaaccaaagg aattcaaatc tgtacaatct
     7201 gccgaaaaga aaaccaaact tagcagactg tttgaatcaa aacatcttgc ttggcaaaca
     7261 tgtggcctta gtatcatggt aactgtccaa attgccggct attttgggtt aatgaattgg
     7321 ctgccttcaa ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc attgtggatg
     7381 gtcagtacta ttttaggcat gtctttgggg atgatgactt ttggcataat catggataaa
     7441 ttggggccta aactagcctt ttctttgttt ttaatttgtt catccttatc tgtctatcta
     7501 cttgtgttag cccatagcca gtggagtttg gtagcagccg ctgtgatagt cggctacttt
     7561 atcaatggca tgtatggtgg atatggcgca gtgattagta gtttgtatcc aacagaaatc
     7621 cgcgcgacgg cgaataattt cattatgaac cttggacgag ctgttggtgg tttttcttct
     7681 atcgtcatcg gtttcttgat ggatcattat agtttaactg ccgtaatctt gtttttgagc
     7741 ggtatttacc tagtcagtct gctggtcttg acaactctta ctggtgttac agcattaagg
     7801 gaaagcttgc agcattgata gttgagtctg taaatgacaa gaaagaggtg tggcaaaagc
     7861 ttgcatctgc ggtaataaga ccagccaaat caaaaacatg agacactgtt tccgtttggc
     7921 tggtcgtatt acttgatgca gggcagcttt ttcaactgct aatttccttc tatctggcgg
     7981 cgactattct ttgacgtccg tcatttcaaa gtactttact aataacttag cataacgaaa
     8041 aactgtggat acactcgtat attgagcgtc tgcataatct tccaaatttt tctcaggatg
     8101 ttgatacatg aattcaatca aatgataatt atatgatttt ctgtagaaat gaagataaat
     8161 aatgtagatt gggaagttct cctgcttttc catagaaaag cgccgattat tctcattctt
     8221 caaaatgacc ttatcaggat atttttggaa caattcttct aaatctgtta gataattcaa
     8281 gatagactta cttgatacct tcaattcgtt ttgtagttct tctattgaac accaacgttc
     8341 tgtttcaagc agtaaatgaa tcattttaat tttcttttga gaaacttttg ttaaaaaagt
     8401 ctgcatattt tctcctcctg tatctacttt actatactac atacaagaaa aaaaacggat
     8461 acaaatat
//
LOCUS       DASJTM010000111         8248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJTM010000111 DASJTM010000000
VERSION     DASJTM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.112"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="U4B29_002516"
     CDS             234..1559
                     /locus_tag="U4B29_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2107420.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="U4B29_002517"
     CDS             1552..1902
                     /locus_tag="U4B29_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107421.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="U4B29_002518"
     CDS             1899..2069
                     /locus_tag="U4B29_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107422.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="U4B29_002519"
     CDS             complement(2973..3248)
                     /locus_tag="U4B29_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN2107423.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="U4B29_002520"
     CDS             complement(3220..4173)
                     /locus_tag="U4B29_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2107424.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="U4B29_002521"
     CDS             4785..6278
                     /locus_tag="U4B29_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2107425.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="U4B29_002522"
     CDS             6413..6709
                     /locus_tag="U4B29_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2107426.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="U4B29_002523"
     CDS             6810..7661
                     /locus_tag="U4B29_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107427.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="U4B29_002524"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="U4B29_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DASJTM010000112         8101 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJTM010000112 DASJTM010000000
VERSION     DASJTM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.113"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..875
                     /locus_tag="U4B29_002525"
     CDS             174..875
                     /locus_tag="U4B29_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2107428.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1484..2500)
                     /gene="tsaD"
                     /locus_tag="U4B29_002526"
     CDS             complement(1484..2500)
                     /gene="tsaD"
                     /locus_tag="U4B29_002526"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN2107429.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2497..2961)
                     /gene="rimI"
                     /locus_tag="U4B29_002527"
     CDS             complement(2497..2961)
                     /gene="rimI"
                     /locus_tag="U4B29_002527"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2107430.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2968..3510)
                     /gene="rimI"
                     /locus_tag="U4B29_002528"
     CDS             complement(2968..3510)
                     /gene="rimI"
                     /locus_tag="U4B29_002528"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2107431.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3494..4216)
                     /gene="tsaB"
                     /locus_tag="U4B29_002529"
     CDS             complement(3494..4216)
                     /gene="tsaB"
                     /locus_tag="U4B29_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN2107432.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4407..5387)
                     /locus_tag="U4B29_002530"
     CDS             complement(4407..5387)
                     /locus_tag="U4B29_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2107433.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5411..6895)
                     /gene="galT"
                     /locus_tag="U4B29_002531"
     CDS             complement(5411..6895)
                     /gene="galT"
                     /locus_tag="U4B29_002531"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN2107434.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6907..7896)
                     /gene="galE"
                     /locus_tag="U4B29_002532"
     CDS             complement(6907..7896)
                     /gene="galE"
                     /locus_tag="U4B29_002532"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN2107435.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2297 a         1724 c         1416 g         2664 t
ORIGIN      
        1 aaatcattcg cttcaaaaca aattattcaa taccattgta tattttttac ataataacaa
       61 attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat
      121 tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg
      181 ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct
      241 cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac
      301 agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg
      361 ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa
      421 ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag
      481 cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa
      541 aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg
      601 aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat
      661 tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc
      721 cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag
      781 taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct
      841 gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc
      901 tgataaatat cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt
      961 tttagatcac tggacaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca
     1021 ctgtttgcaa aaaatactgt tagattaact agatgaagcg tcttgtcctc atccgatcta
     1081 gattattcga tattcctcga caataaacga ttactcagtg agggcaatca gaggaacgga
     1141 taatgataaa taagctgtaa aaaatagcaa aataacaata atgattaaaa gtatgtaaaa
     1201 aatagggctt tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc
     1261 aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt
     1321 ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag
     1381 cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg
     1441 ctgttcttat tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca
     1501 agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg
     1561 ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt
     1621 ctacctcagg gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg
     1681 ccgctacacc tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg
     1741 ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt
     1801 ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc
     1861 cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat
     1921 cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata
     1981 ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt
     2041 catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg
     2101 ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat
     2161 ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac
     2221 caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt
     2281 ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt
     2341 gatggcggct agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag
     2401 aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat
     2461 cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa
     2521 ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc
     2581 taatatacga aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac
     2641 ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg
     2701 ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc
     2761 atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag
     2821 ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc
     2881 atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa
     2941 atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa
     3001 cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa
     3061 tttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc
     3121 acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt
     3181 acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct
     3241 tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag
     3301 cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac
     3361 ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta
     3421 acgacgatcg atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct
     3481 tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca
     3541 accatttttc ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg
     3601 caactggttc aactaaagca cctaattgag ccacagttac accacttgga atatcccatg
     3661 ctggaactgt attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt
     3721 gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat
     3781 cagaagcaga aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt
     3841 atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca
     3901 caccgatgca attagccgcg atggttttca gactagatac tcctactagc tctttatcta
     3961 atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg
     4021 gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca
     4081 attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt
     4141 gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta
     4201 ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag
     4261 catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga
     4321 ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact
     4381 ccattttccc acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt
     4441 tccacaataa tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc
     4501 actgctagct ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca
     4561 tatttcgcta cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa
     4621 cctttttctt gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc
     4681 gcatcaggaa catgcggatt cgtttttaaa tactgcttca ctgcttgata tcctgactcg
     4741 acagaaaaat ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt
     4801 tgaaatgcca taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt
     4861 ccagcaagca tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga
     4921 acacttgact taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct
     4981 accaaaagca aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt
     5041 cctagtgcga tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat
     5101 aaagtaacgt ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa
     5161 agatcttcaa gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta
     5221 tttgctgcat ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt
     5281 tgctttgtgg ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta
     5341 gcaggagaaa ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc
     5401 ttttttacgt ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc
     5461 gcttgaatac tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta
     5521 cgacatcggt gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc
     5581 acggacgatg atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta
     5641 ctagttctgg tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa
     5701 tgttctcctt tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt
     5761 cttctgaaac attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc
     5821 gtgcaattgg cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata
     5881 ctgtttcatc cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca
     5941 agtttttctc tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga
     6001 tgacttgcgg ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa
     6061 attcatgacg tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta
     6121 agtcagcatt cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag
     6181 caaatgtttc ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt
     6241 cattgtaata agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc
     6301 gaataatccg gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt
     6361 ccatgcaaag catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt
     6421 gttttggatc tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg
     6481 actctgccgg aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct
     6541 tacttaaatt aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa
     6601 agaatgcatt gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa
     6661 tctcttgttc tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct
     6721 gatccaataa gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat
     6781 cttctcctac catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc
     6841 cgccagcttc gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc
     6901 attcccctat tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat
     6961 aatcgctttg atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact
     7021 tgaagcgact aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc
     7081 ttttcctgtc acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct
     7141 gcctaaattg aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg
     7201 tgcagcaatc aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc
     7261 ataatcgtct ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat
     7321 tgggacaaga tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc
     7381 ggctacattg aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt
     7441 catcattttt tccatcatca atttactttc accatacgga tttttaggat tagctggtgt
     7501 atcttctgtg attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac
     7561 gattttattc acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt
     7621 gttgttgaaa tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa
     7681 atggattact ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg
     7741 gatgtctcct tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa
     7801 attatctacc acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc
     7861 gatataaccg gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa
     7921 aattgtgttt gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca
     7981 aacaaagtaa aatttcgtaa attcatatgg taaatttata ataaaacatt tagtaaacat
     8041 tttcaaggat tttttgcaaa ggtttacaaa caaacgtaaa actagccttt ttttctcttt
     8101 t
//
LOCUS       DASJTM010000113         7979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJTM010000113 DASJTM010000000
VERSION     DASJTM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.115"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..409)
                     /locus_tag="U4B29_002533"
     CDS             complement(<1..409)
                     /locus_tag="U4B29_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107436.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIR"
     gene            577..894
                     /locus_tag="U4B29_002534"
     CDS             577..894
                     /locus_tag="U4B29_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107437.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            827..1306
                     /locus_tag="U4B29_002535"
     CDS             827..1306
                     /locus_tag="U4B29_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEN2107438.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1323..1499
                     /locus_tag="U4B29_002536"
     CDS             1323..1499
                     /locus_tag="U4B29_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107439.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1756..2187
                     /locus_tag="U4B29_002537"
     CDS             1756..2187
                     /locus_tag="U4B29_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107440.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            2193..2510
                     /locus_tag="U4B29_002538"
     CDS             2193..2510
                     /locus_tag="U4B29_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN2107441.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2629..3315
                     /locus_tag="U4B29_002539"
     CDS             2629..3315
                     /locus_tag="U4B29_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2107442.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            3290..4639
                     /locus_tag="U4B29_002540"
     CDS             3290..4639
                     /locus_tag="U4B29_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2107443.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4630..5103
                     /locus_tag="U4B29_002541"
     CDS             4630..5103
                     /locus_tag="U4B29_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2107444.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5078..5605
                     /locus_tag="U4B29_002542"
     CDS             5078..5605
                     /locus_tag="U4B29_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEN2107445.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            5620..7932
                     /locus_tag="U4B29_002543"
     CDS             5620..7932
                     /locus_tag="U4B29_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2107446.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         2827 a         1355 c         1769 g         2028 t
ORIGIN      
        1 ttcttatgac cattacaagt ttttcaccag gtaatatatc tttcatatct aaaacttctg
       61 aaaaagcgac tgagtagcca ggagagattg cttgtgccaa cggtatagga gataaaaggc
      121 tacttttatg aatgcttccc atatcaaaac cattggcgtg ataatgtgaa caattaattt
      181 tcttaccgtt gtaaagatac gtacattcga taattgagtt tggcattttt gatgggtttg
      241 caattacgcc agtaagaatc attcgattac ttctatcatg cttaactaaa aaataggaga
      301 gcgtaatttt tcttttatct ttaaataagt tatagaggga taaaaaaaat cctgaaatag
      361 aaatgattaa agtaatagcg acttggtaat cgctcaaaat ttttatcaag taattcacca
      421 cctttttact caaattatat caaaaaggag agaagaaata atgcaagaat tagtaatttt
      481 gaaaaataaa aaaagacacg aacgccggca agcatgatcg tgtcagggaa ataaactatc
      541 tttctatatt ttaccacaga aaggaatgaa ccgtaaatgc taattgcaac ggatacacta
      601 gaccgcatct ttttaaaaga cgaatacaaa ctgcgcaata tagatgcgtc aggaatttta
      661 gtatttgatc tttatgacaa tggaaaaatt ggtatctatc aagcaagtga tattgaagaa
      721 acaaaccttg ctttcgaaca aattgatgat tctgtggaat tggatttaga tgaggcaatc
      781 ctagcttttg aacaaattgc aaaattatta aaggaggcac aaaagaatgg caactcttta
      841 ccaactcagc gagtcatata tcaaagtcct ggaactggca gaacaattgg atgaagaaat
      901 tcttcgcgat actctcgatt cgattaatga agcgatcgag tataaggcag aaaacttagc
      961 aaaaatagtt aaagaagtag aagggaaagc tgagttaatc gatagtgaaa ttaaacgtct
     1021 gcaggaacgt aagacatcgc ttttgaacaa tgctaagagt atcaagcact atttacaaga
     1081 ggaaatggaa aagactggta aaacgaagat taaaggtgaa ttattcaaca ttggaattca
     1141 aaataatcca gtatcggtca atgtaatcaa tgagaactta attccaaaag gattctttac
     1201 ccctgtgcct cccaaattgg ataaaaagca attgaaggag gaactgaagc acggagatat
     1261 tcctggtgct gaactcgtcc aaacaaaagg tttgaggata agatgatgaa ggaggagaaa
     1321 taatggaaca cgaaaacgta aaagacgctt tgaaagctgc tatcgaaatc gcggaagcta
     1381 aaggaataaa ggttgacggc aaaccagcaa ctgtccatga cattcaaaat ttgacgaaag
     1441 aacatcttta ttttatcgcc gacttgcttg gtttgtcgga attgtattta gacaaataac
     1501 cccacacgga aggagtggct agggtggttg tattacaagt aatagtctgc gtaacattca
     1561 tttggtcgtg cataccggtt tgtatatata tcatgaagaa gcttcgccct gagtcttaca
     1621 aattatacgc aatttacagc ataacagtag ctcttgcatt tacattgttt gcaatttcta
     1681 ggcttccgtt ttaatcgtgg gaatgaattt ttaatgaact aaattatcta taaagcaaaa
     1741 aaaggagaaa caacattgaa aaacaaacta gcaaaaacaa cagcaatcat cggactagca
     1801 ctaggtagcg gcgttatcgg ctacgcagca agcaatgcgt ttcaagattt ggacacgatc
     1861 aaagcaaatt tcaacacagt cctacaatac ggtcaaacga aatcgcaacg tgtgtcagaa
     1921 cttgaatcac agctatccaa caacactcgc acacaggagc agctgaaagc cgaaattgag
     1981 caaatcaaat cggacaagca gaaggaaatt gaagccaagc aacgagaagt cgaacaaaag
     2041 caacaggaga tcgctacaaa gcaacaggaa gccgatagct tgcgccaaca actatccaca
     2101 gtgcaaaacg acaaggagca gttagaacag cgcgtgagcg agttacggca gtatacggat
     2161 caaaaggtag gggagttggg gaaatgaaag agttgactat ttatgaccca aatatttcat
     2221 gggctaaaca cacaattaaa gtttctttta tgatttgggg ttacaaagga tatgttactt
     2281 acaaagtggg cggtaacact aagggtttat cattaatagc tattgattca gatgatctat
     2341 atgatgctaa tttcgaggat aacccagtta atttccgtga ccttgatgaa gattggttct
     2401 ctatggaact tactaacgat aaaggcgaca gtacacttgt agaagatgaa ttcgacagac
     2461 taggtgatta tattgtcggt gttgaaatta ttgcgcacga accagagtag gagagtgaat
     2521 acaaaatgat taaaagaatc actatatcag ttgaagaaga tagtggatca acaatgcagc
     2581 ataccgacaa tcgaaaaact tttttaacca atagaaagga gaattttgat gcagattaag
     2641 aaggcgacgg atatcgaaca aaccaaggga acatatttga tctacggtgc tccaggaaaa
     2701 ggtaaaactt caacggctaa atattttcca gggaaaacac tgatccttga tattgatcgg
     2761 acttcaaaag tccttagagg tgagaaaaat gtcgatatcg tatatatcga caacgaagat
     2821 acctggaacg actggggcaa cacgttggct gatttaacta ccaattatgt aggtgtttat
     2881 gacaacgtgg ttgtcgataa tgtcagcgaa ttggaacggt gtattctgtc cagtttaggc
     2941 gcagaaggaa aaaacaacgg cgttccttcg caaggcgatt atcaatatat gcaatttaga
     3001 atggtgaaca gcctacgcta tatgaaaaat ttagacagca accttatctg gaccgcatgg
     3061 gaagaaatcg atttgtggac ggattcggat ggatcttctt acaacatcgc tttgccgcag
     3121 atcaatcgaa agattcgaaa caatattttg ggattatgtg acgttgttgg tcgattgatg
     3181 gtaaaggaag acgatgaacg cggatttatt ttacaagcca cagattcaac ctatgccaaa
     3241 aatcaattgg ataatagatc tggctgtaag caaagtgagc tgattttaga tggaattgta
     3301 cgactaccag aatgatttag tcgatcgtgc aagacaagcc tatgttgatg gttacaaggc
     3361 accatgtatc gtatcaccgt gcggttctgg aaaatcagtc atggttgcag agatcgctaa
     3421 acgaaccaca cgaaaaggca acagagtgtt gttcttggtc catcgtcgtg aattgattga
     3481 tcagatcaaa gatacgtttc aaaagatggg cgtagataat cagcttgtaa atttcggcat
     3541 ggttcaaaca gtcgtacgac atcttaaaac catcaagaaa ccacaactga tcatcactga
     3601 cgaaaaccat cacgggttag cagcgtccta cagaaaaatc tatgagtatt attcggacgt
     3661 tccgcgatta ggttttaccg cgacacccat ccggttaaac ggtagtgggt tgggggatgt
     3721 gaacgacttg ctaattgaag gagtatcggc taaatggttg attgagaatc atagattagc
     3781 tccttatgaa tattatgcac ctaaattgat cgatacagcc gaattaaaga aagcttcgac
     3841 tggagatttt acaaagaaat caatggataa ggcagtgaag aatactattt atggcgacgt
     3901 gttgaaacat tataaaacgt tagcagaagg tgaacaggca atagcatact gtcacagtat
     3961 cgatgcaagt aaacacactg ctgaaatatt caacgatgct ggttataaag caacacatat
     4021 cgatgcaaaa actccaaaag atgaacgtgc agatattatc gaagcgttca gaaatcatga
     4081 aattaaaatc ctatgcaatg ttgatttgat cggagaaggg tttgatgttc ctgattgttc
     4141 gactgtaatt atgttgagac ctactcaatc gttatcattg tacattcagc aatcgatgag
     4201 ggggatgcgt taccgaccag ataaaacgtc aatcatcatt gaccatgtgg gtaatgtgaa
     4261 ccgttttgga ttgcctgata tggatcgtga atggagttta gatacaaaaa agaaaacaaa
     4321 aactgatagt gatttatcta tcgttcaatg cgcgttttgt tttggtgctt acgaacggcc
     4381 aaaaggcgat aatatttgtc cgtattgcgg agagttgcag ccgattgaag aacgaaagtc
     4441 agagttagaa atcgatgaat cggctgaatt aatgaaagtc ggagaaacga aaattacttt
     4501 gaattttgag aacaataaat attacaacat gacggaagat gaagcagaaa gtatagagga
     4561 cttatatgcc atagcaagag ctaaaggatt taaacctggt tgggcttata tggctgctaa
     4621 gaggaagggg tggttgtagg aatgggcggt atttttggta aaaaagcaga agatgttcaa
     4681 ggttcaataa taaatggcgt aacaataata ggtgatactg gaaaaagaga tagttcaggc
     4741 aatcaaattg taatcgctag aaataaaaat aacgaagttt ttgaatcttc tctatctaat
     4801 ataaaaagag gtgttgttaa cgggacaggc gaagagagtg gctacgttaa tttaaaaaaa
     4861 gctaatgaaa aataccatgt tgaaggaact tacgttaagg ctttgtctgt cggtggaaca
     4921 aaaggcgtta attgggataa acgaagaaat aaatggaaag cacgattata tttcaaagga
     4981 gaatatgtct tacaaaagga tttcaacacc aaagaagaag caatccaagc acgacttgca
     5041 gcagaagaaa aatatttcaa acctatctta gaaaaatatg aaaataaaaa ggagaatgat
     5101 taacatgaca ggatttaact tagacttttc aaaagcgcaa caagggaacg aaatcaaaga
     5161 cggtacgtac gaagtggtcg taaacaaagc tgtagaaaat gcaactaaat caggcgcaga
     5221 atttatcgat atcgatttga tcgttcgtaa cgatgtggat caaccgttcc aaaataaaca
     5281 cattttcgca aaaatctgga aagccaaagc taccggcaaa tataacgaag gtatgatcat
     5341 ggcaattgca caagcgctac agttggaaga cggaaaatca tataacgggt ttgatgaatt
     5401 gctggcggac tttgttttga aaaccgcctc agtacgcgtg aaaactgaag agtcaaatgg
     5461 atataaaaac gtcaatgtga agtcttggga taaaacgaac acacggggcg taatgaatca
     5521 ccaattcaaa aatggtgatg aaccatcctt cggaccggaa cgttcaagag cgacaacggt
     5581 tagaaatgac aatcttccat tttaattgag gtgtaatcaa tgtatgaaaa aattccaaaa
     5641 gagcttaaaa atctcaaaca atggtgtgtt tataagctag tttgggatga aaagcggcag
     5701 aagcacacga agatccctta taacgccaac aacgggcata aagccaaatc caatgacgag
     5761 agtacctggt ctgattttca gaccgctctc gctgctattg atcactacgg aatgagtgga
     5821 ctggggttct ttttcaaacc accttatttc ggcattgata tcgataatgc tgaaggtgaa
     5881 gttgagcgat ttaaatcagg tgatatagaa gaaaatatta tctatgaatt tatcgaaagc
     5941 atgagatcct atgctgagta ttctcaatca ggtactggca tccacattat tgctcgcgga
     6001 gaattgcccg gcggaagacg caggaaaggt gacgtagaaa tgtaccagaa cggccgattc
     6061 ttcgttatga ctggaaatgc cgcatcaaaa tatttagaaa taactgagcc gaatccaaag
     6121 gatatcaaac gcctatatga tcgctacgtt ggagatgaaa aaatcatcca attcaaagaa
     6181 gaaaatccgc tgatgaatac cgttgattta ccggttgaag aaattatcaa acgtgctgaa
     6241 tcgtcttccc aaggggcaag attcaaagtc tttatgaacg gcggttggga agctttttat
     6301 tcctcgcaat ccgaagcgga tatggcgttc gccaacgact tggctttttg gaccggtaga
     6361 gactttgaaa aaatggatga gattttcaga gcgtctgcaa tgatccgtag caaatatgac
     6421 caaaaaaggg gatcggcaac ttacggagaa tctctactaa ataaggcaat tgctgataca
     6481 aattccgtct acaaccccaa acggaaatct gattttaaga tttttatcaa agaccaggaa
     6541 cagccaaaag aggaaaaata ctattcgtac gacgacaccg gcaacgcgga tcggtttact
     6601 gatatctacg gaacgttagt caaatattcg tacattgata aatcttggta ttactacgat
     6661 gggaaagttt ggctacagga caataccggc gaagttcgga aaatgatcga tacgacagta
     6721 gacatcatgg gaaaagaacc gttaacgatc cctgaaggcg cagacgatga aaccaaagaa
     6781 gctttgatga aagcgaaaga aaaacacgtc aagcgttctc gcagcaatgc cggaaaaaac
     6841 gctatgatgg acgaattgaa acatcgacta tccgtattgc cggaagagtt cgacaaagat
     6901 aaaacgctgt tcaacacaca aagcggctat ctgtctttgc atgacggtct tctacacgag
     6961 catgagatag acaaaatgtt tacacgtgtt tcaaatgtcg agtacacaga atcggtcgat
     7021 tgtcctatgt gggaagaatt tattaatcag atttttgaca atgatcaaga attaatcaga
     7081 tatatccaga aatgtgtcgg atattccctg accggatcta ccagagagca atgtatgttc
     7141 atcctctacg gtcatgggtc aaatggaaag tcggtatttt tggaaattat ttctgaattg
     7201 atgggaaact atgcgatgac tatgcaggca caaacgatca tggtcaagca aagccaatca
     7261 tcagcaaaca gtgatattgc acgattgaaa ggtgcgcggt tggtcacttc gtcagaaccg
     7321 aatgaaggcg tccgactgga cgaaggtctg gtcaaacaat tgactggtgg tgataaagtc
     7381 acggctcggc acctttatgg aaaagaattc gagttcgaac ctgaattcaa gttgtggctg
     7441 gcaacgaacc acaagcccat catccgtggg accgatgacg gcatctggcg acggttaaat
     7501 ttaattccat tcactgtcca aatcccggat cacaaaaagg ataaaaatct gaaatttaaa
     7561 ttgcagacgg agttgcaagg gatccttaaa tgggcaatcg atggttgcct gttgtggcag
     7621 agagagggct tggagaaacc aaagtcggtt gttgcagcga gtcaggatta tcgaaacgaa
     7681 atggatcaaa taggtacatt tatcgaaacg tgttgcgaaa caggtccagg actaaaaata
     7741 tcaggcggag aattatacaa agtttatcgt gaatgggcat cagataatgg cgagcatact
     7801 ttcactaaca cgaagtttgg cagagagatt tcaaagaaat atagcaaaga aaaagctggt
     7861 ggatttatgg tttataaagg aatcacatta aaaccaagaa aatacgataa cgttagagaa
     7921 ctttttaaat aaatctggag ggttctggag ggttgagaac gaaaccctcc aacccttag
//
LOCUS       DASJTM010000114         7732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJTM010000114 DASJTM010000000
VERSION     DASJTM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.116"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            112..2439
                     /locus_tag="U4B29_002544"
     CDS             112..2439
                     /locus_tag="U4B29_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2107447.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2565..2894
                     /locus_tag="U4B29_002545"
     CDS             2565..2894
                     /locus_tag="U4B29_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN2107448.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2934..3938
                     /locus_tag="U4B29_002546"
     CDS             2934..3938
                     /locus_tag="U4B29_002546"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2107449.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3935..4381
                     /locus_tag="U4B29_002547"
     CDS             3935..4381
                     /locus_tag="U4B29_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN2107450.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4531..5346
                     /locus_tag="U4B29_002548"
     CDS             4531..5346
                     /locus_tag="U4B29_002548"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2107451.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5552..6724
                     /locus_tag="U4B29_002549"
     CDS             5552..6724
                     /locus_tag="U4B29_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2107452.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6886..7380)
                     /locus_tag="U4B29_002550"
     CDS             complement(6886..7380)
                     /locus_tag="U4B29_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107453.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7377..7571)
                     /locus_tag="U4B29_002551"
     CDS             complement(7377..7571)
                     /locus_tag="U4B29_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107454.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2620 a         1453 c         1533 g         2126 t
ORIGIN      
        1 aataaaaaga gaaaaaaagc cccaaacttt ctatacatca ataaaataat aaaaattaaa
       61 tttagaataa cgtgtaaagg attatttgat gattttttat tgagaggagg gatgaaaatg
      121 attaagaaaa ctcaaaataa aatccaacgt atatgtcaaa aacattttgt ttctagactc
      181 aaagctgtat ctaaaagaaa atacttacat cccattatga tttcgatcat cttgattttt
      241 actttcttag ggagcttatc aagtatttat gtaaacgttt atacagaaac gtcaaatttt
      301 gctaaccaaa cgacagagga gacaaatagt ccaccctatt ctgaaataca gtccatagaa
      361 agcaccaatc agtctagcaa caacgagaca acctcaacca catcctcgac tcaactgatt
      421 gatccgacag aaaaagaaat cacagcagca aaagcagcag gggagaaagc ttattcagaa
      481 acaggtagaa gccagacatt tagtactgtt gcggccactg cgaatttcaa cggacatacc
      541 ataccagatg gatatacagt gatatatgcc aaagatggag tcattgttgc ggttaacgga
      601 tcgatgagtg cggataaaac tgcgttacaa ggcgtaacca aagcaatcgc ttttattgat
      661 aaccgtccag atatccataa tctgtttatt ggctccacag taaatggtac tactatcaca
      721 ttcccaaaaa gtatctatat gggggtggct accgctggaa atacgatcga ttcagttaca
      781 cccaatgcgg ataaaagtta ttcttttaca ccctcaacat ggggagggct tcctgccgga
      841 ggaggtatga gtactttttc aatcggtata cagttagcaa atgatattcc attcacgggg
      901 tactcgatca aaaatgggca gatcgttgat ggtaagggaa atgtttcaaa ttttccaacc
      961 tatcaagcag caacggttca ggtcaatgcg aatcggacag gaactttttc ttatacagtc
     1021 aatggtggag cagcaaaaat cggaacgtac gggactacat tatctggaat ccatgtgggt
     1081 gacaaagtgg ttatcactcc aacagcagta acgggctatt cttttgcaca aacaaacggt
     1141 tctttcacag ccctagaaag taacgtcgat acggtgacct atacaggcaa acaaacacca
     1201 atcactatta agtatcaaga ctattttgga cagatgattg ctccttctaa aataatgaat
     1261 cttacttacg gaagtgctgc acaagacctg acgactaata cgcctactat caacggatat
     1321 acctttacag ctgtttcagc gatagaaaca aagaaccaat cagcaatgag tgttttagct
     1381 agtttagata caaatggaaa tgtaatcgta aaggacgcaa atggaaaaca gatttctgaa
     1441 gtcatcctat attataagac aaatgcaaca gtatcaatca atgcaaatgg cagtaagtac
     1501 tatgatggtc tttctgttgt accgatcgtt aaatatgcct ttaatgataa aacagaatca
     1561 acatcccttt taaacaatga tctcagtatt gatcaaatca catggaatcc aactgatttc
     1621 aaagcgatga atgaaaaggg agttgaagta actgcgccta ctgaaattgg aacctatacg
     1681 tcttggcaat tgacaaaaag cggactagct aaactagctg ccagaacaaa ctatttattt
     1741 actatcgtgc agacatctga tgtctatacg attaagcaaa tcagcggtgc ggtagagctc
     1801 agtggcagta agacatatga tggaaaagca ggtgtcccgt caatccatgt caagctagca
     1861 gagggtgtca ctagttcgct tactccagta caagtggcac ttagcagtat ggattacaca
     1921 gcagatgcac aatcagcaaa aaatatggtt aatgctggca gctatacaat taaactaacg
     1981 aatagcggaa tagacaaagt caaagcagca aatcccaagt tgtcatttac tgatatagcc
     2041 aacacatcag gaacttatac catctataaa gctaacgcag tgattactgt cgatgacgct
     2101 gcctttaatt atgatgccca aagtcactcg atttcggttg gaaatctcca cgttaccggt
     2161 gttgtcttag gcgaatcatt ggattatacc ctaacagtta acagccggac agatattgga
     2221 tcacaaatag taacgattag tttaggcgaa ggaagtgtaa acaataatta tacgattatc
     2281 gcaacagata ccgctaagtt gacgatcaat ccgactctct tgcttccttc gacaggtggg
     2341 ataggtctac tgccctttgt ctttttagga ctgatcttta ttttttctgg cttcttttat
     2401 tttatccatc gtaagaaaaa agcaggtgaa cagcgatgaa aaaaacaaaa aatgtacgtg
     2461 tggatatatt tgctgttctc ttttttatcc tactggtctt ttttataggt tctccgactg
     2521 tgtcagctag agaatcacct attacattta ctttgcataa tcgaatgttc gaaagcgata
     2581 agatgccaag tatcagtcaa aacgacggaa taaatatccc ggataacttt caaggagtaa
     2641 aagggttgaa cggcgttact ttcgctatat atgatgtctc ggatgaattt tacaagctgc
     2701 ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact agcacaaaaa agtgatagtg
     2761 aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga ggaagagggc attgcttctt
     2821 ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt ttaccgattt acggaaataa
     2881 agacttcaga ttaagtaaaa gaaaaatcag caccatttgt cgttgtgctt cctgtgacag
     2941 attcatctaa tcataaattg acgaccatcc acttgtatcc aaagagcgaa caaaaaatac
     3001 ctgctgcact cacattaaca aaaacagtag agaataagca gacagatttt gcagatgggg
     3061 ataaagtccc ttatctcatc accgtaacaa ttcctgagaa catcaacgag ataggaacct
     3121 atacgatcaa agatacagct gatcctcagt tatggctaga accagaaacg atcgattttt
     3181 tgattggtgg agacaagatt catacatttc acacacaaaa aataaaacat ggttttgtct
     3241 tatcagattc tggaaaagat cttagttcgt ttgctggaaa aaaattaacg atccgttacc
     3301 aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga taacgatgta cgtttgacga
     3361 ctgacaatca aacagtagaa acacatcttg ccatccaaac aggaggcaaa caatttgtca
     3421 aagtggatcg gcaaacgaaa caaaaactag cagatgctca atttctagtc aaaaaaaatg
     3481 accaatactt agcgcaagaa aacgggataa atcattgggt agccaaagat gattcaagag
     3541 caacgatttt tacttccagt aaagatggaa cattttctgt ccatggattg tcttttggaa
     3601 gttacgaact agtggagatt aaaccgccta aaggatatat attgagtcaa tcaaccattc
     3661 tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc tataccattg gaaatcatca
     3721 acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc agttcagatg cagacaaatg
     3781 gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac aagcgaagag atgcttggag
     3841 gtttctcaat tttagggtta ttgctagtcc tttcaacagg gacagcttgg ttttataaaa
     3901 aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa aacatcaaaa caaaaatatg
     3961 gacgttttta ggaactgcta tcatgctgct tcctttcgtt ctcggcctag gaactgcaga
     4021 ggtttctgca gccgtatcac ctacaccaga aaatgttacg gttaatttgc ataaattgaa
     4081 attcacttca gcaccagaaa atcaaataaa caacggaacg gaattgacat ttccgaatag
     4141 cgaaccgttg aacggcgttg aattcaacgt atatgacatc actgctacgt actatccaag
     4201 taaagacaca gcagttccag cagacgctac acccttcgca agtgtaacga cttctggtga
     4261 aggattagct aacctgactc ttccaggtaa atctgatggg aaagatgctg tatacgtctt
     4321 tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat atcgtgttaa gcttacccta
     4381 gaccctgaca aacctttaga cacgattcac ctatatgcta aaaatgtcat caaagaaagc
     4441 aatatcaaaa tccagaaagc tgggaacttt tcaggggtag ataagttagc aggggcgcaa
     4501 ttccagatca aaaattcaga gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc
     4561 gctctctatg atcctgctga gtcagcgttg acattcacaa ctgatactga aggatccatc
     4621 agtgtcaatg gattaataga cggcaattat actttagttg aaacaaaagc accagatggt
     4681 tatcaattaa ccaatccaca aaaagaaact acttttatag taaaaaacgg aaaattgcaa
     4741 caaacaaccg gtatcatcca tggcgcagat gaagccaatc ttgggaatcc aaacggtgta
     4801 gtcaatcggg aaaatacaac tacggataac acgattactg tagaaaatgt ccaaaactac
     4861 ggtgatttcc acttcatcaa acaagatgtg aacacgcaag aaaaattagc cgatgcagaa
     4921 ttttctattc taagagcaaa agatccaact gttaaattaa tgaaaaaaat agcaaacctt
     4981 gcctcaggag aatacccata tgcttggtca gatgaagtca caggttctga ctgggaagaa
     5041 gtgaaattga tttctgattc agaagggaaa ttttcaatcg aagggctgaa acaaggggca
     5101 tactgccttc aagaaacaaa agcaccagaa ggctacgcat tacctaagaa tgaagctgcg
     5161 tacactgaat ttacagtaga tgatgatcca acaacttctg atgaaacaac gatcgataac
     5221 caacctaaag gaatcttgcc ttcaactggt ggtatgggga tcatcgtatt catcttggta
     5281 ggtactgctt tagttggagg agcagtgatc tatttcaaaa aacgccatca agaaacagaa
     5341 gcttaaacta ttggaaacaa aaatcatacg ataaataaac gatagtgatt caaatgggga
     5401 gaaaggttgt gacaagtggt cggtctcttg gtattcgctc aacaaacggc aaaataggag
     5461 gaactatttt gccgtttgct tcgctaatac tcaggtcaag gcgctgattt cacaacctct
     5521 tcatatacat attagaaaat aggtggacat gatgaataaa agcagaataa gaatactgga
     5581 catcttcatg gcaatcattt tagtcgttgg gatcgggatc ttttcttatc cttttgtaga
     5641 agattcactg aatgattttc ttgcccagca aatgatcatc cattatcaaa agcaagcatc
     5701 gaataagaat tcagaggaaa taaaaaaaca gcaggaaaaa atgacaaaga aaaaccaaca
     5761 gttggcagag caaaacgtat caccagggat aggcagtttc aaccaagcag ttgatgcaaa
     5821 agcattaaaa gatttaccta gtaatgcctt ttttatggaa catatgcttg gagtcatcga
     5881 aatcccaaaa atcaatgtca gcttacctat attcgatcag acaacggaaa tatttttgca
     5941 aaaaggaacg tcattattgg aagaaagttc ttatccgact ggcgggaaaa acacccatgc
     6001 cgttctttct gggcatcgtg gactcccaga agcaaaatta tttacagatt tgcctaagtt
     6061 gaaaaaagga gaccagtttt tcatccagat caatggaaag acattggcgt atcaagtaga
     6121 aaaaatccaa gtggtcttac ctgatgaagt agattctttg ggtatccaaa aaggcagaga
     6181 tctcgtgact ttgcttacct gtactcctta tatggtcaat acacatcgac tgcttgtgac
     6241 ggggcatcgt attccttatc aagcaaaaga ggcaaaaaaa gcgatccaag gaattgatca
     6301 atggaaaaaa tggaaatttt ttatttggtt tatcggtata ctacttggat caatcggctt
     6361 agtttggcta ctcatcgctt atttagattc tttggccatc gctaagcgaa actatccttt
     6421 atctttttat gtaaagaaca caaacggacg accaatcgaa ggaatggtct tttctgtaaa
     6481 aacgctcaac ggcaaacact atattactag agaaaaagtt ccatttgtga aagcttctga
     6541 tgaatacgga ctagtgagat tttctgattt aaaaggcagg aactatcgct tgcaacatga
     6601 ggagctcctg cttaaaatcc atgtaaaaca taaacattcg aaacagtttt caatgaaatt
     6661 gaaaaaagga agatataaac tgcgaaaaga aaaagaggtg tactatttga ttgagaaaga
     6721 atgaatgaag atgtccaaaa aagcagaggg aacagactaa atgaaaaaag agagaagctg
     6781 acgaaatgga cgtgtaaact gttatttttg gtttactatc tggaacgcca tcttctattt
     6841 ttttatgttt cagaagaatt ttattattat agtataactt ttgaattagt ttccataaca
     6901 tttggagcaa ggggttaaac cacgagcttg agcttcgctt aaagtggcag gggtatacgt
     6961 accatttcca catttatgtg tatggtattt tgaaccggtc ggtgtgacat agacagtttg
     7021 ttcattattt tgctcaggag ctgcggcttg tgcctgctga gaggcctgtt cagctgctga
     7081 ttgctgtcgc gcctgttctg cttgttgctg tgcgattgct tcttgcctcg cctgttctgc
     7141 ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc
     7201 ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag
     7261 acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc
     7321 atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac
     7381 tttttttcta gtgcagcttt ttccgctttt tgtttctctt cttttttctt ttcaatttgt
     7441 tcttgttctt tcgatgaatc tgccgtactt tcggagatcg tggtactgct gctggaagcc
     7501 tgatctgaac ttgtgctttg agtagtagac gtagtagcac cacacccact caacaaactt
     7561 cctgcgacca aaaacaatgc aactaatttt ttcgctaaat ttcctcctta agtaaaaaac
     7621 aaccgttttc aataaatgtc cttttcaatt gtttacttac ttgttataat aattgtgtta
     7681 tgaatagtga ttgaggaaac ttaatctaat cccgtgttga tacacgggat tt
//
LOCUS       DASJTM010000115         7648 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJTM010000115 DASJTM010000000
VERSION     DASJTM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.117"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..1288)
                     /locus_tag="U4B29_002552"
     CDS             complement(140..1288)
                     /locus_tag="U4B29_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107455.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4B29_002553"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4B29_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN2107456.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1890..3044
                     /locus_tag="U4B29_002554"
     CDS             1890..3044
                     /locus_tag="U4B29_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2107457.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3038..3460
                     /locus_tag="U4B29_002555"
     CDS             3038..3460
                     /locus_tag="U4B29_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107458.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4594..5562
                     /locus_tag="U4B29_002556"
     CDS             4594..5562
                     /locus_tag="U4B29_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2107459.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            5572..6060
                     /locus_tag="U4B29_002557"
     CDS             5572..6060
                     /locus_tag="U4B29_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2107460.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            6356..6637
                     /locus_tag="U4B29_002558"
     CDS             6356..6637
                     /locus_tag="U4B29_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107461.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            6639..7055
                     /locus_tag="U4B29_002559"
     CDS             6639..7055
                     /locus_tag="U4B29_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107462.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2705 a         1318 c         1244 g         2381 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     1861 cctaattctg ttaaatttaa cacaaaggcg tgattatttt gaaacccaaa aagaaaattt
     1921 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
     1981 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
     2041 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
     2101 ccgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
     2161 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
     2221 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
     2281 caaaagaact tcaaaatcta gtagatagta atataacaga tacaacctta ttttttcaag
     2341 ataatagcat ttattattcc gataatggac aaattaaact actttggaaa tcacctttaa
     2401 gtacagaaaa aaatggtgtt tcttccctta cagaaaaaaa tgatcttata tccgtaaata
     2461 taaaaaataa aattaaaaat ttttctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     2521 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     2581 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaac aaaacagatg
     2641 tacgtgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     2701 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     2761 tttatcctaa aaaaatagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     2821 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     2881 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     2941 cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca attcctgtac
     3001 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac tcattgcttt
     3061 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     3121 aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt cagaacgtct
     3181 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     3241 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     3301 ttacaaagca acaaataatt tactaacact gcgctcgaat aaaaaacaat tagattacac
     3361 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     3421 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     3481 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     3541 tgctagcctt ttttgtattg tggagcgtgg gttttgaggg ggattcaagg gggagcttgc
     3601 gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag caaatataga
     3661 gcttgccaag ccctttagtg tgttttgtag aaagggacct tatagtcact ttcacattac
     3721 aaacgatacg tcctatggac gtatcaaaag ggtgggactt atgagcaaat taaagcaaaa
     3781 tttggctagc aatccttcta aaaaatacac ctattgatca gagtcaaaat aaatcagttt
     3841 tcgagtgagc gaatccaaat ttgcaactct gaagctttgc aaatgagtgt gccggcttcc
     3901 gtaaaagtct aggtaacatt agtcgatata tacatagggc tctaatttgc gttttaaggc
     3961 gttttaaacg ttctggtatg taattatacc aaagcgactt taaaacgcct taaaacgaat
     4021 tttagaggtt ctgtagaacg ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     4081 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     4141 ctcagagctt ctaaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     4201 ctgcgcagtt taatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     4261 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa aattttaggc
     4321 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     4381 cgtcggctgt atccttactc tcccaaggtg tgcagaatct tttaggggtc tttttgtctg
     4441 tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta ggggtctttt
     4501 tgtctgtttt taggggtctt taattagggg gttgaccccc attagaaagc tctttataat
     4561 tttgttaagt agttaatctt gaaggaggat tttatggcaa atgaaattgt gaaataccac
     4621 catgaattaa atacgattcc tttacgcaaa tttacgtcag tagaaatgaa tttattcttc
     4681 tctataatat ctagaatgcg tgatgtaggc gataaaaaag tacaatttac attcgaacaa
     4741 ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga tgatttagaa
     4801 acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga cggtttgcaa
     4861 cgtgaacgat ttgttttatt taatcaattt aaaatcgatg gaaaagctga tattcctttt
     4921 gctgaaatac aagtccatga gaaagcctta cccttactta ataatcttga agaatgggtt
     4981 cgatattctc tccaacaatt caatgaatta gaaagcagtt attccaaaac catgtttaga
     5041 ttattaaaac aatacagaac caaaggaatt gccaattttt caaaagaaga ctttcatgaa
     5101 ttacttgata tccctaaaag ttatagagaa agtgatataa ataaaaaaat tataaagcct
     5161 attcgccaag aactaaccgc aatcttcaaa ggtttgacaa tccaaaagag atacggaaaa
     5221 ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat aaaaaaatca
     5281 gacgacttta acaaacatgc tccaaccaaa gaagaacaaa ataaagcttt ggaactagct
     5341 aatcaacagg ttcaatctga aaaagaacct acttcattac aagaaaaaat caaagaacaa
     5401 aaacgaaaat tagaactttt acaacagtta gcagaattag aaagaaaaca aaatacccca
     5461 agtaagaaaa ttaacaaaca aatagcagat aacaattccg atattacccc agaatttaaa
     5521 gaaaacttac tagataatct ccaaaactta tttaaagaat aaggggtttt tatgacagat
     5581 aaaactatca aagaattagc agatattcta ggtgtttcaa aagataaagt gaagtatcaa
     5641 ctgaggaaat tacctagtaa ttttacctac aaaaaaggaa atattaccta cctgaaaaaa
     5701 gaagcagtaa caacaattac aaaggtttta tcaggtaatg aatcgggtaa cttacctagt
     5761 gaattacccg attcattacc taccgattac ctactcaagg agattgaaac taagaataat
     5821 catattgaag aaaaagatag gcaaattgaa aaacttcaaa tagctttaga aacgcagcaa
     5881 aaattactcg accagcaaca acaactcacc ctacaagcca atcaacaaat caaaaaactt
     5941 cacgaacaac tccagctaac ctatgcagaa gaatcttcag aaaaagaacc cctttctgtt
     6001 actgaagaaa ctcaaaaagg acaaaacaag gagaaaaaat ggtggcaatt ctggcaataa
     6061 cccaacaatt tttattcata aaaattgttg ggttactttc tttttttgct tttacaagca
     6121 tagcgacatg atctcacgtc gctatttttt aaagagcata aggaaacgcg gtatcacttt
     6181 cttacgaaag gtgctaggta ggctgtgcct accacgtttc taagaaaaaa atttgctatc
     6241 actttttcgt cattgtggat agcttatgta tagtgtttcg gcaaaaaaca gctatacaaa
     6301 tactatattt gtatgatata cataaattat acacaaatgc gaaaggaatt cttctatgga
     6361 cattttagtt cggaatattg atccaaaagt tatttcagaa atagatagac gttgccaaga
     6421 actcacaaca aaaactggca aaaaatggag ccgtaatgcg tacctaaaag ttctaatcga
     6481 aaacgatttt gaccaggcac ttaggcatta caaaaaagat caattcgatt tattactcga
     6541 aaaatttatc gatatacaag caaaaaatac tgaaacattg aacgactatg ttcatacaaa
     6601 caaccaactc attgaaacac ttatcggagg aatataacat gagaaaaact tctgttcaaa
     6661 aacacgttac cctttatccc agtcacattg acgttttaga ttccatcatc gaaaaaaatc
     6721 cagatatcaa taactactcg gaagccattc gttttctatg tatgagttat gaacctaata
     6781 ctaatacgac aactaccagc aaccaagcaa aactaaatgc gatgggaaaa gaaatttcta
     6841 ttatgtccga aatcattgca gaatttgctg atttatatct aaaagatact ggtgttctta
     6901 tcggtacaaa ttctaaagta tatcaagaag tgaaagaaaa aattgaaaat caactcaatc
     6961 cctcccgacg aaatcaaccc aaaaatcgtc aaaaagaaac caatcaaaca ttcacttcta
     7021 gaaattttga cccaacaaac tttctaaaaa gatgaaaaag aaaccgaaaa ttttttctat
     7081 ttctcagttt cttttttagg gggcagtctt ccagcaagat ttgcaagtta ccggtaactt
     7141 gcacaaaaaa agaaaaatcc gcttgtgcga atccttcttt ttgcattttg gggcgctgcc
     7201 ccaatctcca aatatcggtt atttaatagc gtggtaactc aaaaaaatag aacgtactct
     7261 gaaataaaaa aagactgctc taacgagcag ccaataaaaa ataatgagcg tttattcttg
     7321 aaaaagcagc gcgcttatga agcgctgcca aaaatttacc atattttatg aaaaataaca
     7381 agtttctaaa aaggaatcgg ttaagattca caaagaatcg taaacagaca atagcggaaa
     7441 atcaagcttc cgaacgtacc ttttagctag accaatgaaa aatcacaaag tgagcccctg
     7501 tatcggttga tacaagggct acttggcata ctcgacatta tgtctactag ctcgataaat
     7561 aacgattatg ttcccaaaac aatattttat gaactataac aaaaatttgt tatcgcatat
     7621 tgtatatttt aattataggt gtatatca
//
LOCUS       DASJTM010000116         7635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJTM010000116 DASJTM010000000
VERSION     DASJTM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.118"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..75)
                     /locus_tag="U4B29_002560"
     CDS             complement(<1..75)
                     /locus_tag="U4B29_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107463.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDV"
     gene            213..647
                     /locus_tag="U4B29_002561"
     CDS             213..647
                     /locus_tag="U4B29_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN2107464.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            640..984
                     /locus_tag="U4B29_002562"
     CDS             640..984
                     /locus_tag="U4B29_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2107465.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            1164..2174
                     /locus_tag="U4B29_002563"
     CDS             1164..2174
                     /locus_tag="U4B29_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2107466.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(2243..3187)
                     /locus_tag="U4B29_002564"
     CDS             complement(2243..3187)
                     /locus_tag="U4B29_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2107467.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(3171..5891)
                     /locus_tag="U4B29_002565"
     CDS             complement(3171..5891)
                     /locus_tag="U4B29_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2107468.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(5888..7108)
                     /locus_tag="U4B29_002566"
     CDS             complement(5888..7108)
                     /locus_tag="U4B29_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN2107469.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(7389..>7635)
                     /locus_tag="U4B29_002567"
     CDS             complement(7389..>7635)
                     /locus_tag="U4B29_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEN2107470.1"
                     /translation="IVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADI
                     SFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         2297 a         1444 c         1263 g         2631 t
ORIGIN      
        1 tacatcgaaa ctgatatctt ttaacacagg gagatgtccg tacccccctg tgacatgatc
       61 gataattaaa ctcattcttt ttcctccgcc atttattttt atttattata ccacgaagaa
      121 aatgacggta ttttgttttt ttccttacaa tagctttaac ttatggtaaa gttaagctga
      181 agtatttatt ctttatagaa aggatgtttt atatggaaaa ctgtattttt tgcaaaatca
      241 tcaatcaaga aatcccaagc tataaaatat atgaagacga caaggtctat gcttttttag
      301 acatctctca agcaacgaaa ggacacacat tagttgtgcc taaacgtcat gtagcagata
      361 tttttgaata tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct aagattgctc
      421 aagcattgga aaaagctttt ccagaaatga aaggtcttaa tatcttgaat aacaaccgtg
      481 aactagccta tcaatctgtt tttcattcac atatccattt agtccctcgc tacagcaaag
      541 aggatgattt ttctattcac tttgtcaatc atcaagatag ttatggatcg gaagaactaa
      601 aagctattca agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa ctttttcaaa
      661 ggactgttat ttggagctgc tgctggcact gtcggaggtc tgttattggc gcccaggagc
      721 gggaatgaaa caagaaagaa actgatcgaa gaattggaag aagcaacaac tctgacaaat
      781 gatttaaata acagcctgaa tcattttaaa agcgcattga tcgaaacaaa agaaacagct
      841 gaatctgtga ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga ttttaaattc
      901 caagccgaac cacgtatcac acaaatcaat gaacaagtag aaaaactaaa agctgatctg
      961 ccagagtatc ctgaacaaat ataataatga ttattttctc aacagattta actatataag
     1021 tgagaaaagt ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg tttgaaacta
     1081 ccacgacaac gtttttaaag gaaatgggat atggtatagt taatatataa aaataataac
     1141 taattacagg agtgcaaatt ttaatgaaaa aaaaatcaat tattttggca gctactagtg
     1201 cgttggctgt tttaactcta gcagcttgtt caggcgatac taataaagat atcgcaacga
     1261 tgaaaggcgg aacgatcact gtatctgatt tctatgatga agcaaaactt gaatcatcga
     1321 accaatcctt agtacaacgg atgatcattt ataaagtatt caataacaaa tatggcgata
     1381 aagtaaccga taagcaagtg gatgcagaat acgacaaaca agcgaaatca ttaggtgata
     1441 cttttgaatc acaattagaa gctgctggat atacgaaaga tacttataaa gaatatattc
     1501 gtaacaattt agcttttgaa gctggcttga aagctcacgt agatattaca gatgacgatt
     1561 tgaaaactgc ttggaaatct ttccatccag aagtagaagc acaaatcatc aaattatcaa
     1621 gtgaagatga agcaaaagat gtgaaaaaat cagcggatga tggagatgat ttctctaaat
     1681 tagccaaaga caaatcaact gatacagaga caaaagaaga tggcggtaaa gttaaattcg
     1741 actcaacgac aacaactatt cctgcagaag taaaagaagc agctttcaaa ttgaaagacg
     1801 gtgagatctc cgatgttatt actacaacaa atcctacttc ttatgcgaca gaatactacg
     1861 ttgtcaaaat ggtgaaaaac caaaataaag gcaatgacat ggataaatac aaagatcaac
     1921 taaaagatat cgctactgaa actaaactaa gtgacaatgc attcacaact aaagtaatcg
     1981 gtgaagaatt aaaagatgca aatgtaaaaa tcaaagatga tgcctttgaa aatgtgttga
     2041 gtgcattcac aacaacttca tcaagtacga aagactcaag tgaaactaca gcttcaacaa
     2101 agagttctga cacaaaatca actgattcaa caaaagaatc ttcaactgaa gaaacaactg
     2161 attcatcaaa ataaaagaaa acaaaaaaag cgaagactta tgtctcgctt tttttgtttt
     2221 gaatgaaaaa aataaatttt tgctataaat cttttggtac atagaaactt cggttgtcca
     2281 taccgaaaat ccgatcggtg tattttcctg gttctgtttg acgaatcgaa gtcaacatca
     2341 tctgcatcga agcatcgata ttgtcgattt gatgtaatat ttctgcttcc attattcttg
     2401 gacgtacagg tgagccatat tctaataagc catggtgtgc taaaaccata tgacgtaata
     2461 cgagaacatc ttcgtcagct tcgctgattt tcaacgcaag acaggcttta gttatttctt
     2521 cgtcaatcaa taccagatga ccgatcaaat ttcctgcaag cgtatattct gtcgacatag
     2581 gaccggaaag ttcaatcacc tttcctaaat catgcaaaat gataccagca tacaacaatg
     2641 ctcgattcag ctcaggatat tcatcggcga ttgatttccc taagtgaagc attgtcaaag
     2701 tatgataagc taatccactg gcaaatgcat gatggtttct ttttgctgcc ggaaactcaa
     2761 aaaattgttt ttgatattta ttaagtagat agcgaacaat acgattccaa tgcgcattgg
     2821 tgatctcaaa caacaattga ttgatttctt cttccatttc ttctttcttc aaaggggctc
     2881 tttccatgta taaactaggc tctcctggct catctaccct tgttaagcgc atatgaagaa
     2941 tctttacttg agggtttcct tgataaacct ctcttttacc gtttagaaaa acgactgtgc
     3001 cggctgtaaa tttattgatt tcctcctccg aagcatccca gtatttacca tcgatagtac
     3061 ctgaagtatc ttggaaagta aaagcaataa atttttttcc gtttttagcg attcttacat
     3121 ctgcactttt gatcaggaca aattgttcaa actgctcatc gattactaaa tcacgaattt
     3181 ttttcattat ttgctccttc taattgtttt acgatttggt tcatctcatg gtaatagctt
     3241 accatttctt tatcagaaga aaaacaaatt atttgatact tttctccaaa tttagctaaa
     3301 agttcagcta attgatactt tcgctgatga tcataatgaa gccagccatc atcaatgatg
     3361 atcgggcata tcgaacggtt agcttctaat gagaggtaag caaatctcat cgccataatc
     3421 agctgatctt tcgtacctgt agataactca tagatgggaa attctgtata tccgtctgtt
     3481 aaagtcagtg tatcattttt cagtaatatc ttctgataat ttccttttgt taaaatacct
     3541 aaataatcac ctgctgctct taacaactgg ggtaattgtc tttcactcat ctctgttgaa
     3601 agatcaccta agaatgtccc ggcgatttca agtgctcccc attcctccag taattcctgt
     3661 aattttgctt tttgccggct agcttgttga taaagttcgt ccaatgtgcc gtctctttgc
     3721 aactcttcta cttgaagttg gagtcgttgc cgattttcac ttgcttcttt ttcttcttgc
     3781 tggagttggg attgcttttc tttcagttgg cgcagtctta cagataattt ctcttttgtc
     3841 agctcttcag gaaatagtgg agtaagcatt tccgataatt cattgtatct tttcatctgc
     3901 tgttcggctt gtttggcttt ttgtaaaaag acaggtactt ccgaaggata agataagcct
     3961 gcttgagtca attgttcgct gtattcttgt atccatactt tttgtttctt tttcaattga
     4021 ttgatttctt gcttcaataa agtattttgc tgatagcttc tggcaaattt catctgttcc
     4081 atctcttgta taaaacggga aagcaatacc attttttctg aaattgtctt atcttgtatc
     4141 ggtaaccatt ctttaacaaa ctcaaatttt tggtcgattt gtcgtagttt ttcttgtatt
     4201 tcctgttctc ttttatgttt ccgctcaaat acgccatata atttctgata atctatgact
     4261 tcctgtccat attctttcat gagtttgagc gtatccatct ttcctaagtg ccgatgatcc
     4321 gcaaaacgat atacttcttg ctgaagaatc tgtttttctt ctccagttct cttttgtttt
     4381 tcttctgctt cagctaattg agctaaggct agatcaagtt gtcctagttt ttcttgccac
     4441 attcctttta tctcagagaa atcattttta gttttaattt ttggttgcct tttgtagaag
     4501 aaatagccaa tcatgcctgc accaattagt aaaaggatca ctttcaaggg cattggtaca
     4561 aatatacctg caatgattat tgctccgcca gccatcagca tatacggttt agatgaagag
     4621 actggtgcta tctctgactg attttgtaat aatgctggat aggtctcttc caagtcattc
     4681 acttctgctt caatcatctc ttgctgttct ttagctattt ggagacgcac agaaatctgg
     4741 ctttctagat cagcaatcct tctttcttca gccagaagat gcttccattc ttcttcatat
     4801 aaaaattctt ccggtaacgg atttttctgc caatgccacc tttgttccaa aatcttcaat
     4861 tctgaatttg tttctctagt ctcaatttgt aatctttgcc attcttcgtt taattgttcc
     4921 gctgtaaatt gaagttgctg gagatctttt agttcctgct cctgctctaa ataaaaataa
     4981 tatctggctg actgctgatc catcccgcta tgcttttcca gtgttgcttc taattcttcc
     5041 aaacgctcat ttgtttgctg ataccgtttg gagaaagact ccagatctaa aagaagttgc
     5101 tcatcaggaa gacctgtagt gctgtttttt ttgagagatt gccattcttc atataaagaa
     5161 aaattaagtt cttgctgtct ggctaatgaa aattgtttgt tcgcctcttt taattgtgcc
     5221 aataactgct gctgttgcga ctcgtattcc tgttcttgtt ggatcaactt gccaaaataa
     5281 gcttcctgtt cttgtttttg atgaattttt tcttgcagct ctttccattc atttaatttt
     5341 tgattgatta gtaatttttg gccttttttc ttgaataatt gttgtgcttt ctgataatat
     5401 tcttgccgct tttggaatac ttgagaactg cccgtgatgc ctaaagaaat cagcgcatca
     5461 tgtagctctt tttctctcaa atgatctagt tccatcaact gctcttgctg gaaagtaaat
     5521 acctgctgga ataactcttt cgtcaaaggt gcgatcagtt tttctagtaa ctcatcactt
     5581 cccacttgat cccccagcca caccttcgat tttccgcgat tttgctgctt gtagcgctcg
     5641 acagcaaatt caccatatac aggatgttta agccatattt tgccgccata ggctgagccg
     5701 tcttttggtg tataatcctt ttttttacgg ccttttgaag ggaaaccaaa taatattgct
     5761 tgaataaatt gataaatcgt tgattttcct gcttcgtttc ccccatagat cagctgattg
     5821 cctgaataaa agtcaaaact tttttgacgc cattttccaa aagcagcaat ctcgatcctt
     5881 aaaatcttca ttcctgatcc tcctcaaata aaaaatcttg attaagcagc tgcttggctt
     5941 tttcaagtgt ttcttcttga tattccggca gttcgctcaa tattctagcc gcttcttgat
     6001 gactatatac ttcatttaaa atttgttgga aatcttgagg tgtctgatag tattgaaata
     6061 actgttccat caatttagca gaagcagcaa gtggggtctt cgtataaaaa gtattctcta
     6121 tgagagaaat acgccaaata aagaaatcat ttatcctatc tgagaattcc tctaacaaat
     6181 agtctaatag ctctcctgat tgtatccgtt ccaaaacatc tgtattgaga tggtcataat
     6241 cttttaattt tatctcaatt aaactaaatc catcctctac ttttgaaagc tgctgacgaa
     6301 tgtgaacaat gacctctttt gtcgttcttg ctttcctcaa tgatatttcc tttgtcttcc
     6361 aatagacctc tgctacttta atcggatgta tttggcaact tcctttactt aattcaacta
     6421 gtagaataga tgtagaagct tgttcctttt tagtatgacc ttgaggcgca cctggataaa
     6481 caattgtttg cttttcattt aagaccgttg gcacatggat atgccctaaa gcccaataat
     6541 cgtatccctt ttcttgcatc tttgagggtt ggaaaggagc atagtttcct ttttgagaga
     6601 cgccaggttc cccgtggtac aacccaatat ggtaatctgt gagttctcta gaaggaaatt
     6661 ccatcacttt gtctttttga atccacggtt gacgataact aaatccactg agagagacag
     6721 tttctccact ttttatcgtt attttttttg tctctaccgt ttcggatgta aataaatgga
     6781 catttttagg aaaggagaac caataccgct ctttttgata gaaatcatga tttccgaata
     6841 ttataaatac aggtatatgg ttcttttcta accgtttcat ctgttctgaa aaatgtttct
     6901 gtatttttaa tgaaggacga ttttgatgaa aattatctcc tgcaagtaaa acaaaatcaa
     6961 cgttatttat tattgcctgt tcaatgatat ttgataaaac ggtcagattc gtcttcaata
     7021 gtttttcctg tacagtttta tcaagagttg tcagtccttc aaatgaccga tccatatgta
     7081 aatctgccgc atggataaat ttcaccatta aaaatttcct cctttttttc ttttatcata
     7141 ccattttcaa tgtttttttt catcaataac cgaaaatttg ttcgtattat gcaaagaaat
     7201 tacgataaat ggccgacttc aaaaaataag tttattcgaa aaaaagattg tgacatgatt
     7261 tttgtcacaa tcctaaatat ctatgttata ttcgcttcgt ttgacactga agaagtacta
     7321 tttgtactgg aatttatgct ccatttttcc gtatttagaa ttctatgttc tatactacct
     7381 taattagttt atatggtaag aattctttca aatgcttgag atactccgtc tttatcattt
     7441 gaaacagtga cataatcagc ttttttcatg atttcttgat gactatttcc cattgcaaaa
     7501 ctaatatctg cactttcaaa catagataag tcatttaaac tatctccgat cgcgattctc
     7561 aaaggtttct ttaaattgta gattgattcc aaacgatcaa acaccacacc tttagataca
     7621 ttttgaggta cgatt
//
LOCUS       DASJTM010000117         7623 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJTM010000117 DASJTM010000000
VERSION     DASJTM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.119"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..439)
                     /locus_tag="U4B29_002568"
     CDS             complement(<1..439)
                     /locus_tag="U4B29_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2107471.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSV"
     gene            complement(441..1475)
                     /gene="sppA"
                     /locus_tag="U4B29_002569"
     CDS             complement(441..1475)
                     /gene="sppA"
                     /locus_tag="U4B29_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2107472.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1508..2923)
                     /locus_tag="U4B29_002570"
     CDS             complement(1508..2923)
                     /locus_tag="U4B29_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN2107473.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(2892..5045)
                     /locus_tag="U4B29_002571"
     CDS             complement(2892..5045)
                     /locus_tag="U4B29_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN2107474.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5303..5539
                     /locus_tag="U4B29_002572"
     CDS             5303..5539
                     /locus_tag="U4B29_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN2107475.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5558..5773
                     /locus_tag="U4B29_002573"
     CDS             5558..5773
                     /locus_tag="U4B29_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN2107476.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(6476..7228)
                     /locus_tag="U4B29_002574"
     CDS             complement(6476..7228)
                     /locus_tag="U4B29_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2107477.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7243..>7623)
                     /locus_tag="U4B29_002575"
     CDS             complement(7243..>7623)
                     /locus_tag="U4B29_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2107478.1"
                     /translation="ANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIES
                     QDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINK
                     KNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         2402 a         1376 c         1123 g         2722 t
ORIGIN      
        1 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
       61 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
      121 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
      181 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
      241 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
      301 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
      361 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
      421 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
      481 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
      541 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
      601 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
      661 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
      721 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
      781 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
      841 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
      901 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
      961 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
     1021 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
     1081 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
     1141 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
     1201 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
     1261 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
     1321 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
     1381 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
     1441 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
     1501 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
     1561 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
     1621 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
     1681 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
     1741 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
     1801 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
     1861 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
     1921 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
     1981 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
     2041 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
     2101 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
     2161 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
     2221 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
     2281 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
     2341 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
     2401 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
     2461 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
     2521 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
     2581 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
     2641 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
     2701 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
     2761 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
     2821 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
     2881 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
     2941 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
     3001 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
     3061 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
     3121 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
     3181 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
     3241 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
     3301 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
     3361 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
     3421 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
     3481 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
     3541 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
     3601 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
     3661 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
     3721 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
     3781 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
     3841 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
     3901 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
     3961 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
     4021 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
     4081 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
     4141 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
     4201 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
     4261 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
     4321 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
     4381 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
     4441 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
     4501 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
     4561 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
     4621 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
     4681 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
     4741 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
     4801 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
     4861 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
     4921 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
     4981 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
     5041 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
     5101 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
     5161 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
     5221 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
     5281 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
     5341 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
     5401 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
     5461 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
     5521 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
     5581 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
     5641 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
     5701 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
     5761 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
     5821 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
     5881 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
     5941 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
     6001 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
     6061 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
     6121 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
     6181 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
     6241 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
     6301 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
     6361 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
     6421 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
     6481 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
     6541 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
     6601 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
     6661 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
     6721 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
     6781 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
     6841 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
     6901 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
     6961 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
     7021 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
     7081 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
     7141 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
     7201 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
     7261 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
     7321 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
     7381 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
     7441 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
     7501 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
     7561 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
     7621 tgc
//
LOCUS       DASJTM010000118         7606 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJTM010000118 DASJTM010000000
VERSION     DASJTM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.120"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..1361)
                     /locus_tag="U4B29_002576"
     CDS             complement(111..1361)
                     /locus_tag="U4B29_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN2107479.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(1739..3190)
                     /locus_tag="U4B29_002577"
     CDS             complement(1739..3190)
                     /locus_tag="U4B29_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2107480.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(3511..4275)
                     /locus_tag="U4B29_002578"
     CDS             complement(3511..4275)
                     /locus_tag="U4B29_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN2107481.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4303..5001)
                     /locus_tag="U4B29_002579"
     CDS             complement(4303..5001)
                     /locus_tag="U4B29_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN2107482.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5013..5792)
                     /locus_tag="U4B29_002580"
     CDS             complement(5013..5792)
                     /locus_tag="U4B29_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN2107483.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(5808..6752)
                     /locus_tag="U4B29_002581"
     CDS             complement(5808..6752)
                     /locus_tag="U4B29_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2107484.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6798..7577)
                     /locus_tag="U4B29_002582"
     CDS             complement(6798..7577)
                     /locus_tag="U4B29_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107485.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2125 a         1616 c         1251 g         2614 t
ORIGIN      
        1 taacaaaact ctcatacttc tccatcagtg gttttaacat cattaactgt tcataatgcc
       61 ccccagagga agccgcaaaa catatctttt tgttcatatt aacctctcta tcagtcacgc
      121 tgaaaaatat ctctagtgta aactttttct ttaatatcat ccaagcaggt atcatatcga
      181 ttggcaataa tagaatcaca catcgcctta aactgctcta aatcgttgat aaccttactt
      241 ccgaaaaaag tctctccatc tttcagtgca ggctcataaa tcaccacagt cgcaccttta
      301 gcttttacgc gcttcataac tccctggatt gaactctgac gaaaattatc actgttgctc
      361 ttcatggtca gccggtatat accgattaaa actggatttt ccctgttgac atcatactga
      421 ttatattcag cgtagttgta atagcccgcc atctgaagaa cacgatctgc gatgaaatcc
      481 ttacgggtac gattagattc aacgatagcc tcaatcaaat ttccaggaac atctgcatag
      541 tttgccaaca actgcttggt atcttttggc aagcaataac ctccatatcc aaaggatgga
      601 ttgttgtagt gagttccaat acgaggatcg agacaaacac cattgattat ctgctgagta
      661 ttcagcccct tcatttcagc ataagtgtct aattcattga aatagctgac tcgcagtgct
      721 aaataggtat ttgcaaacag ctttactgct tcggcttcag tgaatcccat aaaaagagta
      781 tccacgttct ccttaattgc acttttctgc agaagtgttg caaaaacatg agcagcattt
      841 aacaagcgct catcagtcat atcagtacca acaataatac gagatggata cagattatca
      901 tataaagcct tgctctcgcg cagaaactct ggactaaaaa taatgttctt actccccgtt
      961 gtctcacgga ctgattcagt gtagccaaca ggaattgtag acttgatgac catgatggta
     1021 tcaggattca cgtccatcac aacctcaata actgactcaa cagcagaagt atcgaaatgc
     1081 tgagtacgac tgtcatagtt agtcggtgta gcaataacaa cgaaatctgc ggatttataa
     1141 gcgctccttg catccaaagt agcagttaag tcaagttctt tttctgtaag atacttttca
     1201 atatattcat cctgaattgg agacttccgt ttattaatca gttcaatctt ttcagggata
     1261 atgtctaccg cggttacctg gtgatgctgc gccaatagtg tggcgatact caaaccaaca
     1321 taaccggtgc cggctactgc gatgttgtac tttttcttca caatatctcc tctttcctct
     1381 cttaagtatg gagaatgtta ctcctacggc tattcatcat gtcaaaataa cacagcttag
     1441 aatgttgata aaataatttt ccagaatgtg ataaaatctc tttcatttat aaaccacaca
     1501 tctaatttta tctattctcc acaagacatg tagagtattc tttaataaca agatacgacc
     1561 ttactaaata cttcaaaaaa ttatcatatt taattcataa atataaatct tcattttaaa
     1621 tgtatgccca aaaatttatc tgtaatgcgt catcaaaatc tagattccaa atctttatca
     1681 atttttatcc actcattcta ccactttagt aatattttct atttataaca ctttcgtcct
     1741 acattgatcc ccccctctga agtacagttt ttactgtctt aaataaaatt ttaaaatcta
     1801 accccatact ccaatttttt atatattccg tgtcaagttt tgctacttct tcaaaatcag
     1861 taatatcact tctaccactt acctgccaca ttccagtaat tcctggctta atagcaattc
     1921 ttgcacgatg atgataatca tattcttcat actctgattt aaggcatggt cgaaatccta
     1981 taaggctcat ctttcctgtt agaacgttga aaaattgagg gaattcatct aaagacgtgg
     2041 atctaataaa ttgaccgatt cctgtctttt ttgttccatc tggaagtatt ttatttccga
     2101 taattcttgg atcaaactcc atcttaaaca taagattatt gctcatctta ttttctttca
     2161 ttaattctac ttttctttct tctgcatcca tatacatact tctaaactta tacatcttga
     2221 acggtttacc attctgacct atacgttctt gtgcaaagaa aattgggcct ggtgaattaa
     2281 tataaataag tggagcaata aagataaaaa taattcccgt tgcaatacat ccaacaatac
     2341 ctgcaagaat atcaattgtt cgttttataa atgcctgttg agttgtcata acattgatac
     2401 ttgttgtaag tactgtatat tctcctatac gaccaataga ttgttttccc aaagcactat
     2461 ctgatacttt tgcaagattc aaatgtacag taaggcccat ttcagagcat cgttcaataa
     2521 attctttcat acacgaagta cttttattca aaataataaa gacttcatct acccatccct
     2581 gacataagta atccgctgca tttttggaat cagaaacaac tgtcactccc tcaacatctt
     2641 caccaatcat gcttttatct atgataatga caccatttat tttatacatt ttatagttat
     2701 gctctttcac attacgaata actacttcag caatactact agatgtaact ataataagtg
     2761 atttgttccc ctcatttttc attttatgtt tcaagtaatg tttccataag aggcgagtta
     2821 tatatgtaat tacaacgtat aaacctccca tcaagaaaag aacgactcta gaataatcat
     2881 ttccaccctg aacactaatg agatacaaac ttcctaacag caccaacata atcgcttgct
     2941 ttacagaaga agcaaattct atatagtacc ctctttttaa tacacctcta tatgcttcaa
     3001 acatgaaaat aactaaaaga tctgccagtt caatgaagac tgccatgttt tgatacattg
     3061 gaatcacata aggattccaa tctacctggc gtaagacata cgatagataa aaagcaagct
     3121 ggatacacaa taaatctaaa ataataaaat caatatgttt aacccatccc cttgaacttt
     3181 tcttgtacat tcctttttac tcccctcttt ttaataaaat aaattgtatt acttgttttc
     3241 tctcctaaat atctttctga aggattattg actcgacaaa ttggcttgtg gatattctta
     3301 tatatagaac atccgtaagc caacttaaaa ctccttaaca aaattttttt actcatattg
     3361 tagatcaaaa caagtatcca agaaatcata ttttatttct tttttctgct tactcaacaa
     3421 cagctaaaag aaaaatcatc ataaacaaat tcaactaagc caacgatccc cttgtgaaat
     3481 caagtttttt cttgatttac gaataaagag ctaaaataaa ccaaacttct tcttttttac
     3541 ctcttcatag actggataac tgatttcatc cccgttgatc agatcctttg cgacttgttt
     3601 catctgtact actttttcct taccaaagtc ctttgcaatc tgctcatagc actcttttaa
     3661 gtaaaaggtg cgcttagaga ccccatgggc atcagaagca gccatctgca cgagattatg
     3721 tttgaccatc gttttggctg ttttctggat gtctttgcca aataccccaa cataacttgg
     3781 cgcagtgagt tgggccaaac atcccatatc taggaacgga agcaaatgat ttgggtcttc
     3841 gcgaaaatgc acgttgcgtt ctggatggac gattactggt gtctttccta gttctaacaa
     3901 ctcaaaaaaa agccgttctg catataacgg cacttccagt gtcggaaatt cgatcagaag
     3961 atatgtatca tctaaatctg taaataatat ttcatctcgg tggatgtctt cgatgagcgt
     4021 tccggtaata cgtacttctt gtccttccaa taacgtcaaa ggcaactgtc ttttctctaa
     4081 ttcggcttgt aacgaagcga ctagagaaat gacttgtgat ttttcattgc tgtattttcc
     4141 attattgtgg tgtggtgtgc ataaaatatg cgtgatgcct tgctgaatcg ctttttcagc
     4201 cattgcaata cttgcttcta agttttctgc cccgtcatcg attcctggta ggatatggca
     4261 atgtagatcg atcatttctc ttgtctcctc tcttcttatt ccttaatttc cgtagtaata
     4321 accagcatct ttgctatgtt ctgctccgtt gtacacaaca cctaagactc gtgcttgtac
     4381 catctccagc agttcttttg ctttagtcaa ggattcttta cgtgacatat tttcacgtac
     4441 gaccaataaa gtgccatccg tttttaaaga catgatctgt gcatctgtta cggctactac
     4501 tggtggcata tcgaatatca ccacgtcaaa taactgtctt gcttcggata ggatctgatc
     4561 cattcttggc gagcttaaca attctgatgg gttgggtggc ttaggtccac taggcaaaat
     4621 cgacaagttc tcgactggcg ttcgttggat cacatctgct acacttcctg atgaactaag
     4681 tgctgtactt aatccactgg cattgttcag ctggaatgtc ttatagacga cgggtttacg
     4741 taaatctgca tcgactagta gaactcgttg acctgattta gcaaaaacga ctgcaatgtt
     4801 tgctgcagtg gtggatttcc cttctccagg acctgatgaa gtcacgacaa tcgttttgat
     4861 ttgctgacca gctgcggagg caaactggat attcgtccgg atcgtacgat attgttcggc
     4921 gattggtgaa gatgggtttg ttaatgtgat caaactgact gcatgagttt gtgctgcttt
     4981 ttgtttctgt gttcgtgcca tttttcttcc tcctagactc ttgagcggct tctgcgtgat
     5041 ggggctgttt tatcgtttgt agcttttggt cgtgcaggag ctgttccttt tttacggata
     5101 ccagcgttca attctttcgc tgacatttct ggaactgttc ctaagattgg gaatcctaat
     5161 gtatctgtca catatttttc atctttcact gtccgatcga gtaattccaa taagaatgcc
     5221 agacctacac ctaccatcat tcctaacatt aagccagtca ttaaattcaa tttattattt
     5281 ggcgaaacag gtgtcgtatt ggcaactgca cctgaaatga tcgagatttt atcaacattc
     5341 atcacatctt ttgcgttctc ttggaaaact tgtgaagttg tgttagcgat ctgttgagca
     5401 gccactgcat tcgtacttat tgcctgaatc gagaacattt gtgaattttc tgcctgatta
     5461 actgagatgg catctttgat ttgttcagca gtcattttca cattgtcttc tgtttctaaa
     5521 cgatctttga cttcattgag cactaaatca ctgacgatca tatctttata cgtattgatc
     5581 atttgcaggt tcgtattgac atcattgaca tttgcagtgt ctgattgtgg cagtgtcacg
     5641 atcaattgtg cttgtgaact gtattttggt gtcaagatga aaaatgttac gattcctgag
     5701 atggccaagc cagcaaacat tgagatgatg attattacca agtgtttgcg taatgtttca
     5761 aaaatttctt tcaagctgat tgtctcttcc attttttctc cactttccta tttattctct
     5821 agattcattt gatcttttaa ttcttttttc acacgatcaa gttcttgatc actgactttt
     5881 tgataggaga tattatcttg catgaagcct tctccttgca gctgatcttg cttaatcgta
     5941 ccaaatgcct cccgataatc taaagcaatt tttttcattt catcaaagtt catgtctgtt
     6001 ttcatattcg actctagagc attcaaaacg gattgatagt tgctgacacc gtttagactc
     6061 aatacttttt tcgctacgcc ttctacgatc tttctttggc gcccttgtct accgtaatca
     6121 ccatttgggt cttcataacg catacgagaa taagataatg cctgatcccc gttcaatgta
     6181 attttaccga ttggaaagtc atagccatct tgtgagaaag tgaggtcatt attgacttcg
     6241 ataccaccaa ctgcatcaac gagttccttt agtcctttca tattgataga gacatagtga
     6301 tcgattggga tgtctacata tttttgtacg gtatccatcg ccatggcagc tccgccgaat
     6361 gcgtaagcgt ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac atacgtatct
     6421 cgaggaatgc tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc aaccatcatc
     6481 gtatctgatc ttccttgatc gacccgacct aaatctccag tatcaatccc catcaataga
     6541 acagagaaag attcttgttt gtccagatca actggtttcg ttcgttctcc ttgggttcgt
     6601 tccactgatt cgtaagtttt ctcaatagaa tgtgagagat ccagatataa ttttgctcct
     6661 actcctacaa ttgctaatac taatacgata agaactgcta aaataatcaa aaatattttt
     6721 ttccctcttg attttcttct tcgtcttgcc atagtttcct ccgtttgcta accttgcttg
     6781 aatcaacgtt ttaactgtca atagcttata ttgctatagc ccatttgctg agcaacagtt
     6841 tgtgcttttg ttgcgtatgc ggctcttatt ttttcattcc ggatctgctc tatttgactg
     6901 agcgccgtca aatattgatc gtaagtagcg gtagctgtta cttgaccgtc tttcagcatc
     6961 gtatcgaata gctgatctag ttgcgtgact tgtgcaattt gggtatcagc gaatcctaga
     7021 tattgcataa cgactgtttt ccaagcacta tccttaaaaa agctcatatt ttcacgtaca
     7081 tgggcgacat ctgcacttgc caacttttct ttgatgatga catcatccac agcttgctgc
     7141 cagtttgata cgtttttagt aaataattta ttgacagccg tctggatctt cagttttgat
     7201 tctgcatcct ctaactgcgt taagactgct ttcttttctt cttggatcgc agcagcttct
     7261 ttaggtaatt ctgattcctt gatgtcataa tcatctgccg aaagtttgat agaagatagt
     7321 ttatgtgaca aatttgaaac tgtttcttct gttatttcag gatttaggaa aattttttct
     7381 tttgtactgt acagcttatc tacttctttt gataaagagg tcacggtgtc tttttgattg
     7441 gtgatggcag attctgcgta attgatccgt tgatgatatt gccgctgatt attgagatgg
     7501 tatccaaaca agcaaatcac aatagcgcct gtgcctaccg ccacagtagc caataacttg
     7561 acgaacggct tttccataga ttcactccta aatattgatg atagat
//
LOCUS       DASJTM010000119         7537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJTM010000119 DASJTM010000000
VERSION     DASJTM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.121"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="U4B29_002583"
     CDS             join(107..335,335..1248)
                     /locus_tag="U4B29_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2107486.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="U4B29_002584"
     CDS             1483..1935
                     /locus_tag="U4B29_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107487.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="U4B29_002585"
     CDS             1949..2638
                     /locus_tag="U4B29_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107488.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="U4B29_002586"
     CDS             2763..5114
                     /locus_tag="U4B29_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN2107489.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="U4B29_002587"
     CDS             5194..5589
                     /locus_tag="U4B29_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107490.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="U4B29_002588"
     CDS             5914..6531
                     /locus_tag="U4B29_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107491.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="U4B29_002589"
     CDS             6572..6871
                     /locus_tag="U4B29_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN2107492.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="U4B29_002590"
     CDS             6905..7072
                     /locus_tag="U4B29_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2107493.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="U4B29_002591"
     CDS             complement(7117..>7537)
                     /locus_tag="U4B29_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107494.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DASJTM010000120         7273 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJTM010000120 DASJTM010000000
VERSION     DASJTM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.122"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1500)
                     /locus_tag="U4B29_002592"
     CDS             complement(<1..1500)
                     /locus_tag="U4B29_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107495.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDY"
     gene            complement(1520..1687)
                     /locus_tag="U4B29_002593"
     CDS             complement(1520..1687)
                     /locus_tag="U4B29_002593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107496.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1724..2413)
                     /locus_tag="U4B29_002594"
     CDS             complement(1724..2413)
                     /locus_tag="U4B29_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEN2107497.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2668..2988)
                     /locus_tag="U4B29_002595"
     CDS             complement(2668..2988)
                     /locus_tag="U4B29_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107498.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3021..4139)
                     /locus_tag="U4B29_002596"
     CDS             complement(3021..4139)
                     /locus_tag="U4B29_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107499.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4168..4962)
                     /locus_tag="U4B29_002597"
     CDS             complement(4168..4962)
                     /locus_tag="U4B29_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107500.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5015..5368)
                     /locus_tag="U4B29_002598"
     CDS             complement(5015..5368)
                     /locus_tag="U4B29_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107501.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            complement(5470..5727)
                     /locus_tag="U4B29_002599"
     CDS             complement(5470..5727)
                     /locus_tag="U4B29_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107502.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            complement(5730..5954)
                     /locus_tag="U4B29_002600"
     CDS             complement(5730..5954)
                     /locus_tag="U4B29_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107503.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6127..7170)
                     /locus_tag="U4B29_002601"
     CDS             complement(6127..7170)
                     /locus_tag="U4B29_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2107504.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2018 a         1523 c          909 g         2823 t
ORIGIN      
        1 gtaatctgta tatagaaaaa tcgcatcaca cgtattcaaa tttttaagta tctttccatt
       61 attttcgctt acatcaaaca ttttaatatt tggtaatagt tcagttaatt tattttgaaa
      121 atttacatgt cctcccacta aaagaatgtt tttatgtttt agataagcta tcatttcaga
      181 cagtgaaggc tcttcagttt taccatttaa tgattgtcta tgttcttgct cctgcaattg
      241 ctttaatact ttattttcaa acctcagctg ttcaagttct tttctgattc cttttaattc
      301 tttaatctct tgcttcaaaa gttcctcttc tttttttgcc gatttgacta aagattctaa
      361 ccgaccgttt tctttactcg caatacttaa ttcttttctt aatttgatta cttcagattt
      421 gttttctttt ctaatacttg caatttgttt tgctatttca taatcagaat tcaggatcaa
      481 ttcttcctta gcatttttaa acaaccgagt taaaacgaac tgatataaca aactataaaa
      541 aactagcaaa tagtcaattt gtcggtactc ggtattctga tcaattgcat atagagagat
      601 taaaacgaaa tacacaatat cattcagctc gctacccttg atctcaaaat tattgactaa
      661 gtaactatca atacccatca aatctgcaaa atctccatac aaagaaaaat atgattttga
      721 caatatatct atatctgaat cgtcaaaccc atttactatt ttcccatcat tgttagtagt
      781 tgctaacgaa gcaatagtga cttttgaatt gggttttaca aatcctatac ctaatagttc
      841 tttcatcttt ttaatttcaa gtttgttatc cttttgttcg gctttatgct gtttcatatt
      901 gtaaagcgga taaaatgctg ttccgtaagc accttttagt acgttatcta taaaataatc
      961 ttcagaacct gttttgaata gaaagttaat cgcagccaaa ttagataaca gctcttcagt
     1021 gttgtgcatc aattttttat catttaataa attatcccca agatatttca ttgcataata
     1081 agggtttgtt tcgctactta tttttaatat tttttgatag ctattcctca cgatattaac
     1141 caaaatatct gtctgattaa ttgaattaca gtacaaagcc actcctaatg ccatataaac
     1201 attcaaatca tgttttgccg taccgttgaa taaaacgtct tcaacttttg aattatctcg
     1261 tctatacttt ttgatcatat ttttgatatt ttgatcattc tcttggtata tttttttcac
     1321 tcgttccaat gccttgggtt tgttggaaac agcaacagcg aagtctagtt ctttgaaatc
     1381 aatatttagt atctgtacct catcacctag attataatct ctatttacgc tcggaaaact
     1441 gctgaaatct aactgttttt tacctatttt tggcgaaaat attccttcga tttctggcaa
     1501 atgtaatcac actctctcct tatttatctt tagaaaattt gtcactattc tccttatatt
     1561 tatcaataaa cctttttaat cgagcaatat aagcgttaac ccttttaggt tcttcttttc
     1621 gccattcatc gtttacgtga ccattttcaa agaaagaagc taaaagagtt tccacttcat
     1681 taaccatatc tgcgatcgta tatttttctg taccctcact caattattca tgaactgtta
     1741 agtgttcata gctaagttca ttcataattt tgttatagcg ttttcttgct ttttcaaatt
     1801 ctttctgttc gtcaaattcc acttgattct tcatgttgtt cttcctctct gtttgatatt
     1861 ttttcacttc ttcaagttta gcatctagtt ccgctttaac ttctgtcact tctttaagcg
     1921 cttgggataa cttatgagac aatttcgcat aagtttctct cttgtattca tttcctactc
     1981 ccaactcttc ggagtagaac tcttctagta gtcttgcaaa attaacattt ctcctgccag
     2041 catagttttt tagcttctca aaatcagatt gaaagacagc aacacttttg taatcctttt
     2101 caccctcaaa atcaaaagca tagtcatact ttcctgaaat caattcatca ataactgtag
     2161 caatatattg agagatagaa atattatctt cacttctatg tcgctctata agattatata
     2221 tatcagggct gacatgaaac gtacttctct tttttgtttg cctttttcta actgttacga
     2281 ccacttttgg aacttcacta tctccattga tctctttgta tttttcaata aataattttc
     2341 tagcttgttc tcgacttttt gccggaattt catctacaat tttatcttct aaataaatat
     2401 agtgtgttct catacattcg tcctctatct tctttactct caatcttcat gatcttagtt
     2461 cttaccttgt ttactcttaa acctttcaaa aaaatctagt caaaatcaaa cttgaaatca
     2521 tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggaacc cttattttca
     2581 aatcctttat ttttgaatga tgatggttat ggaaaacttc ctttgaaatt ctgacaagca
     2641 taactagtca gaactgtttt acttagatta agctttcaaa agatactgat gaagaactaa
     2701 gttctcagta ccttcccatt cttcaataaa aaatatttct ccttctttta cccactctat
     2761 agctaatttc ttataatcaa ataaaccata tgtgtttaag cctttgtctt tcattaaact
     2821 caaaaactga tcccctttta atctttctgt atcatccatc agaaattcaa ctaaatcttt
     2881 atccatgcgt gcaacttttt cgtcttttag tgccattgat ttagcatagt cagttgaatc
     2941 ttttagaata ataacaccta catttccttc cacaattttc ttcttcatat ttacctccta
     3001 ccatcttttc caaagagtat ttagtcaaaa tcaaactcga aatcatcgtc tatcacaaca
     3061 cccttaactt catctttaga tttcccaaag gagcccttat tttcaaatcc tttgttttta
     3121 aattctaaag ctttttcgac ttcttctttg gatattgtat gaactttttt cttaactact
     3181 ccaagtttat acagctcttc aaactctggt acttcggatc tgaaatacaa tccatcagtc
     3241 cgaattttta atagcccata tgcgctatga acgtatttct gttcatatct aaccatacga
     3301 tctctaaata cgcctgctac catggaggaa tacgctcgat tatacatact gtatttatcg
     3361 aaggtgcttg tttctccttc tcttaatgat ttttgtccga tagcaccaaa tactttcgtt
     3421 ctaagatttt tataaagctt caacgtatca ggatcagttt ttaatggctc gattctttca
     3481 aaatattctt tgagtaatcc ttttttcgtc aaaaatgtgt ggctatctag gacttccatc
     3541 tctttaatat cataataatc aaggataagt gttagctcaa tctccgataa ccaaataggc
     3601 tcattagctg ttttgggatt ttcgccataa tccgcaaaca tcgactgtga ttttggatta
     3661 gcatatgttc cttctttggc tgttccttca aaattaacca aaaagaacat atacgttctt
     3721 aaattttcat acggtcttac ttttggtcta tttatcgctc tcaaaatagc ttgatctgta
     3781 ttatattttg ctttcaaata agtgtttgac ggcaaaggat aatttctcat gatcctcgaa
     3841 taggcttcgt tgaaatcata ttcgatcatc tttgctcctt cctcaaacaa atccaagtta
     3901 tacatgctac cggaggtact ggctctatag gctgaacttg gaacaaagaa aggtgcatct
     3961 tccggcttat catagcccaa aaaatgtatt cctgtttgct tgttctgacg gtaaatgtcc
     4021 atcaaaagat atttgatcgt actaaaagaa tctaattttc ctcttgtcat ccaccctaat
     4081 ccgacaagtc ttttaatttt attctgcatt tcaggtgtgt atatgctatt gtttcccatt
     4141 tatctgctca tctccggctt ttttattcta gaccctatat ttgttcatta caccacttat
     4201 ctcgtctaca aaggctccca gatcgtgtgt aaaggcatcg tagtaatctt caatctcact
     4261 cgattcagtt ctccggtttc ttccgttttt atttctgaat agatacatct gcgtttctct
     4321 taaatccctt gcttttttcg caagtgctaa taaatccgta aaattatcaa taaccataat
     4381 gtcattgatc ataaaacctt cttcatgacc atacttttca gccatctcga taaaatcatt
     4441 aacatcgaac aactccaata attctgcttt atatttctct aacaatgtat ttaaatagcg
     4501 atttctcaat tcccatgaac tttcaccagt tccgtattca accttgatcc ctaaattctt
     4561 agtgtagcct tttgctgtgt atcgtccaat gaagtctccc ttattccgat tgctatcttc
     4621 ttccttgaaa aactcacgcc aaattccctt tttcttttta ggaatcagac ctctgctctt
     4681 gaaatcatta tatttttttg aagcttccct tcgtgtatct aagtaccttt gcttcagctc
     4741 tgtagctgat tcattcgttc catattcaac agtgatcctt aatcgtttgc tatagccttt
     4801 tgctgtatat cgtccaacaa agtcaccgcc acttcgatca ctactgactt ccttaacaaa
     4861 ttctctccat ataccttttt tgtctttcgg tattaatcct cgtcttttca tatcttgata
     4921 tttttcagaa gcttctttta gagtattctt ttttgctacc atgtcatcac ctcgcaattt
     4981 agttaacttt atacacgtct acaaaaaaat atgattactt actttttaaa ttgaaaaact
     5041 cgttgaaatg ctcttctttc aacacaacaa aagcatgata acttaacctt aaaatataat
     5101 ctccatattc aactggataa tctttggtta taactctaat taccgctctt ccgctattta
     5161 caagttcact ttttttcttc ccccttcgta ctcttcgtat actatgaggt tttacaaatt
     5221 cgactacttg cttccaatta tcatttttat attgaataca cagtggtgat tttttcattt
     5281 ctatatacat gttcttataa tcttctactt tcttattttc tttaactaga tgtttattat
     5341 tctcataaga caaattttcg cttatcattt aatatttccc ttcctttttt tgcattatat
     5401 ttagcaaaca ttcaccattt gaatttgaca tagctttaag acagatattt tatcttttca
     5461 aacattaagt tatttgtctt tttcagaagt ggtataggat cgaagtcctt cacgtttcat
     5521 tatgtcttct gcttttccaa tgctgatccc tttccaatca cgccatccta cgcttccctt
     5581 attttgacga taggctttaa tagctgattc ggatatttgc catcttgtag ccagctcttt
     5641 tgcggtgtac ttatttgaga aaagtaactt ctctatcagt gaaatatcaa tcattttatt
     5701 ctcactcctg aaatcttcat taatcatctc tagctagcta tatcttcaat caattctaat
     5761 atgtcggata aaccatcaac agcttcttca agttgatcaa cggattcttc tgctttttca
     5821 tatctttctg atccctgtag atttttaggt atattttctt tgtactcttc ttcttgaatt
     5881 acttctaact catcctttag ctcggaaagt cgctcgctta tttcttctaa cctttttctt
     5941 cttctttggt tcatcaaaat cacctcttcg cataatctct acgattttaa tataccactt
     6001 ttgtgtacta cattcaatat tttttgttat agattcgtat tacaaaaatt tcctctaaaa
     6061 aaataagagc ctatttacca tttcagtaat taagctctta tttcatttag ttgataattc
     6121 tatcaattaa cgtacagcaa ccgccattcc agtcacatta aatgctctct catacgtgct
     6181 tgcattgatc cattttgcgc caaatacagg gtcaacaaca tggtatgcac catttctgaa
     6241 gccatccacg agaactacat gtccattcca tactgcacga ccccaaggca tttgtttgaa
     6301 ctctggcgta gcatagttat aagtaatcca agttacaacc ggattaccat tgcgaatttc
     6361 attcttgatt ccattcgttc ctgaaccagt gatattgacg gcattaccgc caacgttttt
     6421 tgcccattta actactgggt cagccatcat gccttgatac gttccattaa cgttatgacg
     6481 ccactctcct gagtaaccat gataaggatt attgtcatta gcttttggca tatagtttac
     6541 aaaactggtt aagctttgat ttgtggctac acctttgtag tgtaaacctt ctaataaaga
     6601 agctccttcg cataacattg tatttccttg ccatacaaca ggaacgttca acactttttc
     6661 agtgcttgga gcaggtgtag gatttggtac gctgtttggc attgcatacc agcctgtacc
     6721 ttcaatcttc caaccttgtt tactaattac gttcttttca ttttgatcta acgtgtagtg
     6781 atgccaattc acgtatttgt tatataaacg tgttacagga actctatttg ctccgttgta
     6841 ggaatagaac gcaacacctt cgtacgtcca tcctgtgtgt gaaatcttat ctctttctcc
     6901 ggcatcaact gtgtaaaaat gttcaccgtt ataccgatta tacaaacgat aaacaggagt
     6961 accacttgtt ggagcttgcc atgcgacacc ttcgtaatgc catccataac gtttcagatt
     7021 gtctctttct ccaacgcttc tagtgtatag atgctctccg ctattgggat tatacatacg
     7081 atacatggaa atagagcttt ctgctgaaac tgaaggtgat aataatacta gccccattgt
     7141 gaatagaatt cctactaaaa attttttcat ttgattttac cccttttcat tttttattgt
     7201 cattattgca tgaaaattat tcgtaagatt gccaattaca taaacgattg ttttaagatc
     7261 ataatcgttg ttg
//
LOCUS       DASJTM010000121         7251 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJTM010000121 DASJTM010000000
VERSION     DASJTM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.123"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(902..2113)
                     /locus_tag="U4B29_002602"
     CDS             complement(902..2113)
                     /locus_tag="U4B29_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2107505.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(2231..2866)
                     /locus_tag="U4B29_002603"
     CDS             complement(2231..2866)
                     /locus_tag="U4B29_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN2107506.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2949..3371)
                     /locus_tag="U4B29_002604"
     CDS             complement(2949..3371)
                     /locus_tag="U4B29_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2107507.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(3389..3709)
                     /locus_tag="U4B29_002605"
     CDS             complement(3389..3709)
                     /locus_tag="U4B29_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107508.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            4002..4193
                     /locus_tag="U4B29_002606"
     CDS             4002..4193
                     /locus_tag="U4B29_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107509.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            4207..4458
                     /locus_tag="U4B29_002607"
     CDS             4207..4458
                     /locus_tag="U4B29_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107510.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            4434..4613
                     /locus_tag="U4B29_002608"
     CDS             4434..4613
                     /locus_tag="U4B29_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107511.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(4656..5126)
                     /locus_tag="U4B29_002609"
     CDS             complement(4656..5126)
                     /locus_tag="U4B29_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107512.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            5213..5911
                     /locus_tag="U4B29_002610"
     CDS             5213..5911
                     /locus_tag="U4B29_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN2107513.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6089..6430
                     /locus_tag="U4B29_002611"
     CDS             6089..6430
                     /locus_tag="U4B29_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107514.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            6423..7094
                     /locus_tag="U4B29_002612"
     CDS             6423..7094
                     /locus_tag="U4B29_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN2107515.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            7100..>7251
                     /locus_tag="U4B29_002613"
     CDS             7100..>7251
                     /locus_tag="U4B29_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107516.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIR"
BASE COUNT         2360 a         1157 c         1284 g         2450 t
ORIGIN      
        1 gttagtcaat ctaagcggga atgttgtttt atttggagga attaccctag ttttttcgat
       61 aatgattttc ataataggtt ttttagtaag aaaaaatcaa aaaacagtgg tctatggtat
      121 tagtttactc attttatcta ttattccatt ttttctttta acattattgt gggtaattcc
      181 tggagtttta ggtattattg cgggattgat gcttctaata agaaaatctt aattaggcaa
      241 tgtgtagaca acgtgaaacg attaatcaat taaaagctaa atacgttttg agaaatgata
      301 acttgtagtc gatggaagac atgtagataa aaaaactacg tgtcttttat ttattctaat
      361 tttttcgaaa aaaagacact ctctgattgt tgtattaatg gaaaagggga aagcagcaag
      421 taaatttagt ttttctatta aatattcttc actttttaac cttttatagt acttagattt
      481 aatcctatat gtttagagag ttcattggca gcatgatttt gcaaagcttc tcgaattatt
      541 gttatattag aaactttact gttcataatt atgcttcttt tttaactcaa agtccaaagg
      601 gctacagtgg actttgagtt aaaaataaaa agccctagtt attttgaaaa ataaaaaaag
      661 aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc gtgggtttgg
      721 cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt tcgttattta
      781 atggtatttt aaatgaagat aagcgaattt acagcatttt taaatgctta tataccgcaa
      841 attatcccaa gtttttatat atcctacccg agtttatacc cgaaaatata attttttagt
      901 gttattttat atcttttttt aatagttttt caaacgctgt agttgctgta tctttttctt
      961 tgcgtaagac atggccataa acattcattg tagtattaaa atctcggtgt cctaatcgtt
     1021 tttgaagctc ttttggagtc atattaggat cgctaagcaa ataggtagct gatgtatgtc
     1081 taatgtcgtg aaaacgaatg tgacgcaaat tgtgacgttt ggtgaatcgt ttgaacttct
     1141 tatacagata acttccacgt ggtaaagttc cgtcttgttt tgtatcaaat ataaaatagt
     1201 gtcttaattt ctttatattt aatttccaac gaattttttt attctctttg atcaattcat
     1261 ctaacatatc aaatagataa gcaggaccgg ttactatttt ttcatcatca tcatttttca
     1321 aacctggcag caaccgaata tctgattttc catccacttc tgaaatccgc tgatgaaatc
     1381 ttatttcttg tttgtcaaag tcaatatctt tttcttctaa agctgcaatt tctccttgtc
     1441 ttgctcctgt aataaatgct agaacaatta aagttttaat ttctatgctt tcctcaaatg
     1501 ctgcttttag cattgcatga atttcttctt ccgaataagg ttcttcgata ttctttttta
     1561 cgcctttttc ttttggtatt tttacatttt ccacaggatt ttctttaatt gctccgtact
     1621 catttttagc catcaaaaat aagttgttaa cggcgtataa catacgaagc tttgtatttc
     1681 ttgataaagg tttatcagag tttcttccag gatcttttgt attgattctt tgagcattaa
     1741 caacaatttt ctttatcatg tatggtttta tatcgattag agggataaaa tcaaatttat
     1801 caagaaatct attctcgatc attcgcttat agttatgata agactttggc tctaaatttt
     1861 tttcggcctc tgaaagccat atttttctat aaaattcacc aaaagttata ttgaataaat
     1921 ccaacgaatc atcggtcatt ccttcgaatt gctctaacca gatgttcaat tctttatatg
     1981 ccgctctttt actattcgct tttatagttt tgctttttct ttttgccgag ccatcaggtt
     2041 tgtaccctaa tacagctctt agtctatatg tttcattgtc tacttgttct aagtatcctg
     2101 ttacttctga cataattgaa tcagctctct ttctctgata caataggtac tataaagaag
     2161 cctattgtgt aggtttgttt tttcttagaa cacgctcgct ttggtcggtg gggcgtgttt
     2221 tttatactaa ttagttatga agagcaattg aaactgtctc tgttccaaag taacctgttt
     2281 gtgcttgcaa tacattattt tgtgcatttg cttgggcttc agagatatca aaaactattt
     2341 taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta gtgatattac
     2401 cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa gtttttccat
     2461 ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct gcatttttta
     2521 cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca gatgggccaa
     2581 cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca gcaacgtcat
     2641 caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt tctttattag
     2701 tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga gcaccaccaa
     2761 taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa aacggttttt
     2821 taaccttata cattttccca tcttgaccca taactttttt tgccatttaa atatccctca
     2881 cttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg ttgcagcacg
     2941 ggcttttttt actgtgcata agagtatttt ttcttgaaat atgactggca aacattaaaa
     3001 cattctgttc ttaacttatt attgatagag tagaattcca tgaaattttc taatttgaac
     3061 tgagattcat cagtcaattc gttctcaata aagatattta gtaaaatcat aattgcgatt
     3121 ctatcagctt cagcttcgaa ttttgaatga aaagttgtag agttatcgta cagtactgaa
     3181 tattcaaaat gtgaagcaat gaaatgaccg agctcatggg ctaaatgaaa agcttcagaa
     3241 ctgtcttcgt gtagtttttc attcaaaaat actattcttg gttttggata ataaaaacct
     3301 ggttcttcca tttccatata gattaacttt aaattatact cactcagcat ttctttcaac
     3361 tttaaataca tacaacccat cactccaact attcattttc ctctaaagct ttagcaattg
     3421 caatcgcttt acgcattgtc tccttagata tttcttttcc atcaaaagaa aatacagtat
     3481 catcttctga taaatccaca tgtttaggcg tttctttttc ttctctacct agaagatagt
     3541 ctacagagac attgaaatag tcagctacct tttgcaaatc tcttgctttg ggttctgttg
     3601 ttttccatcg atagaaaaga ttttcgctaa atcctaattc taaagcgact tctttcatgc
     3661 ttttatctct ttggctagct aatgatttta tcctttcaaa cagtgtcata ataatgttct
     3721 ccaatcctaa acaaataaaa gtacacaaaa tgtaaattat actgttgaca taagtataag
     3781 tttgtattat tatttgtttg taagttaatt tgatagaaaa aaagcaaact aaaaatacac
     3841 cttatagcat taagtttggc gaccgattgc ataataaagg gtttgttgta ggcttattta
     3901 actatgttta tatagtatac gattgtatac tattagtcaa tagtttatac gttttttcta
     3961 tcattttgac ttacaaatct atatgtaagg ggagtgaata gatgtcaaat ctagataacg
     4021 gccgtgctgc aatcaagaag tttatgcgag aaaacggagt aactatggaa gatttggcta
     4081 ctgcatatgg atacacacgt gttcgaatgc agcaaattat tgatggccat tggagtggac
     4141 caaaagctaa taaaactatc ttagaaatca tccgtgatta cagaattcga taggaggaat
     4201 taatcaatgg aacaactagc tttagttaac ctctcagccc tcaaagcttc tttggcagaa
     4261 tctgagattg caaatgaagt gtgggataca aagcaagctg cggaatattt gaaaaccacg
     4321 acacgcacgc taactaaaga tgccgaatct ggaaagattc cagcagcgaa agttggcaga
     4381 gagtggaggt ttagtagtat cgctttgtat cagtatctaa aaggaggaaa aacatgggca
     4441 aattcaacag agcactagta ttcagcgcac cgctaatcat ctacgcttta gggctttggg
     4501 gaaatagaca agcgttgata ggaacgattg tttacatggt ttggattttt atggggcttg
     4561 atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac taaaaatgaa
     4621 tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt cactaatttc
     4681 tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt tctatttaga
     4741 gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt ccctacttca
     4801 aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac agcttcagag
     4861 tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa tggagtagct
     4921 gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat agctggttct
     4981 gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa gtaagaagca
     5041 ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt taaaaacgta
     5101 ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa aaaattcaaa
     5161 ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa taatgcaaga
     5221 attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg cagaaagctt
     5281 cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag atgtgtccaa
     5341 ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa atcgacgagt
     5401 tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag gaaagaaagc
     5461 tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg ttattcgcaa
     5521 gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag aaatgaaaga
     5581 tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac aacaagagtt
     5641 tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa aagaaagccg
     5701 agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac
     5761 cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt
     5821 tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac tgaacagaca
     5881 acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata gggacggcaa
     5941 ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa catattgaca
     6001 agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag agtcacaaag
     6061 aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc tagatcaata
     6121 tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc gaaaaatcag
     6181 acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag gcaaccatgt
     6241 aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata aacctgaata
     6301 tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca gagataactt
     6361 gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg aggcagaatt
     6421 tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt aagttaaata
     6481 aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg tgggagatta
     6541 tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac tacagggttg
     6601 tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt atggatgaaa
     6661 ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc gaattatttc
     6721 ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa atcaataact
     6781 cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta tatgtatttc
     6841 aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac atgctagaga
     6901 cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat
     6961 atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa actattgaac
     7021 agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag agaaaagaat
     7081 tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag cggttctgaa
     7141 aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca ttgatcgact
     7201 aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt t
//
LOCUS       DASJTM010000122         7217 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJTM010000122 DASJTM010000000
VERSION     DASJTM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.124"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="U4B29_002614"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="U4B29_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN2107517.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="U4B29_002615"
     CDS             complement(919..1677)
                     /locus_tag="U4B29_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2107518.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U4B29_002616"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U4B29_002616"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2107519.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U4B29_002617"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U4B29_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN2107520.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U4B29_002618"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U4B29_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN2107521.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="U4B29_002619"
     CDS             complement(4433..5290)
                     /locus_tag="U4B29_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN2107522.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U4B29_002620"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U4B29_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2107523.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DASJTM010000123         6973 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJTM010000123 DASJTM010000000
VERSION     DASJTM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.125"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..189
                     /locus_tag="U4B29_002621"
     CDS             <1..189
                     /locus_tag="U4B29_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2107524.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="U4B29_002622"
     CDS             complement(275..1369)
                     /locus_tag="U4B29_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2107525.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="U4B29_002623"
     CDS             1558..2319
                     /locus_tag="U4B29_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2107526.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="U4B29_002624"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="U4B29_002624"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2107527.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="U4B29_002625"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="U4B29_002625"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN2107528.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="U4B29_002626"
     CDS             complement(3959..4972)
                     /locus_tag="U4B29_002626"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN2107529.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="U4B29_002627"
     CDS             5137..6057
                     /locus_tag="U4B29_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN2107530.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(6337..6837)
                     /locus_tag="U4B29_002628"
     CDS             complement(6337..6837)
                     /locus_tag="U4B29_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2107531.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2134 a         1391 c         1220 g         2228 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtcccacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
     6181 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt
     6241 aaacgctttt gtcccagcct ctttttcaag ataaagtcaa aactggtacg ctgttttttc
     6301 tacttatttt ttgattatct tttagtttct cgttctttag ctcacgatat cgatttgttc
     6361 ttttaattct tcgattttcg gagacaatat attacgacgt tctaattcat gcaccatgaa
     6421 gtttacggct ttaagcagct cttttcttgt aatgattcct ttgaaaatcc gatcttctgt
     6481 gacaaccggt aaaaaaggcg aatcgactaa caaatggagt acttcttcta gttcccaatt
     6541 ttcatcaatt gtcggaacat ctgtttctgt cacatcagca accgttagtc cttctaacgg
     6601 gtccatgctg attgtttgca agtcaatcat tttattgacc acatctgata aacttactag
     6661 tcctacaaag tgatcatctt ttcctaatac aggaattttg gaataaccga ccttcgataa
     6721 cacaagtaaa ccatgggaca atggatgatg atacataaca tttgccacat tttctgcagg
     6781 aatcaaaaac gtttcctgat tttccagcag catttctctt actataggtc caatcatctg
     6841 atggcctcca tttctatttc attcaacact actattttac cgaaaccgga tgcaaaattc
     6901 ataaaataaa gcttattttt attaaaaatt agagtttttt tattcagcgc tctctcgaaa
     6961 aagaaaaggc caa
//
LOCUS       DASJTM010000124         6951 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJTM010000124 DASJTM010000000
VERSION     DASJTM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.126"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(410..3115)
                     /locus_tag="U4B29_002629"
     CDS             complement(410..3115)
                     /locus_tag="U4B29_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN2107532.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3421..4368)
                     /locus_tag="U4B29_002630"
     CDS             complement(3421..4368)
                     /locus_tag="U4B29_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN2107533.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4558..4827
                     /gene="rpsN"
                     /locus_tag="U4B29_002631"
     CDS             4558..4827
                     /gene="rpsN"
                     /locus_tag="U4B29_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2107534.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5003..5365)
                     /locus_tag="U4B29_002632"
     CDS             complement(5003..5365)
                     /locus_tag="U4B29_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2107535.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5376..6497)
                     /locus_tag="U4B29_002633"
     CDS             complement(5376..6497)
                     /locus_tag="U4B29_002633"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN2107536.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6513..6863)
                     /locus_tag="U4B29_002634"
     CDS             complement(6513..6863)
                     /locus_tag="U4B29_002634"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN2107537.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         1840 a         1494 c         1274 g         2343 t
ORIGIN      
        1 atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt
       61 ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca
      121 ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag
      181 cttgcatctg cggtaataag cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg
      241 tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa
      301 tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat
      361 ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa
      421 tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga
      481 ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg
      541 tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa
      601 atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc
      661 cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat
      721 cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa
      781 atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg
      841 taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt
      901 gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa
      961 cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag
     1021 ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa
     1081 agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga
     1141 cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg
     1201 gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt
     1261 tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt
     1321 ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata
     1381 ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat
     1441 tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg
     1501 ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt
     1561 ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag
     1621 aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat
     1681 taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa
     1741 cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat
     1801 ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca
     1861 ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt
     1921 tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg
     1981 ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg
     2041 taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt
     2101 ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat
     2161 cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg
     2221 tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga
     2281 tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca
     2341 ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca
     2401 cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga
     2461 ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat
     2521 ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata
     2581 ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg
     2641 aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat
     2701 attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat
     2761 cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt
     2821 cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt
     2881 gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag
     2941 caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg
     3001 catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga
     3061 aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc
     3121 tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc
     3181 ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct
     3241 cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga
     3301 aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat
     3361 tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc
     3421 ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg
     3481 tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc
     3541 tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct
     3601 tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc
     3661 atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat
     3721 acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt
     3781 tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt
     3841 actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga
     3901 cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa
     3961 atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca
     4021 actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta
     4081 agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga
     4141 accaatctct gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt
     4201 tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc
     4261 ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc
     4321 aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc
     4381 cctttttctt taagtttacg tttatgcctt tattttgaca aaaaaaatgt cttttcgttt
     4441 tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat
     4501 ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg
     4561 gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct
     4621 gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta
     4681 gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac
     4741 atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt
     4801 ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc
     4861 gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct
     4921 ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg
     4981 ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac
     5041 taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt
     5101 ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt
     5161 ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt
     5221 ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa
     5281 taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa
     5341 aataaatatc tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc
     5401 cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc
     5461 tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga
     5521 aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag
     5581 tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa
     5641 ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg
     5701 attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct
     5761 ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct
     5821 actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg
     5881 ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct
     5941 ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa
     6001 ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca
     6061 ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc
     6121 gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct
     6181 gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc
     6241 aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt
     6301 gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca
     6361 tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca
     6421 acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg
     6481 tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa
     6541 gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc
     6601 acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg
     6661 atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat
     6721 tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt
     6781 cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca
     6841 tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag aacactctca
     6901 ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa a
//
LOCUS       DASJTM010000125         6853 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJTM010000125 DASJTM010000000
VERSION     DASJTM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.127"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            529..723
                     /locus_tag="U4B29_002635"
     CDS             529..723
                     /locus_tag="U4B29_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107538.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            845..1163
                     /locus_tag="U4B29_002636"
                     /pseudo
     CDS             845..1163
                     /locus_tag="U4B29_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1407..2237)
                     /locus_tag="U4B29_002637"
     CDS             complement(1407..2237)
                     /locus_tag="U4B29_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN2107539.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2445..3779
                     /locus_tag="U4B29_002638"
     CDS             2445..3779
                     /locus_tag="U4B29_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2107540.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3820..4731
                     /locus_tag="U4B29_002639"
     CDS             3820..4731
                     /locus_tag="U4B29_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2107541.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4718..5581
                     /locus_tag="U4B29_002640"
     CDS             4718..5581
                     /locus_tag="U4B29_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2107542.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            5578..6194
                     /locus_tag="U4B29_002641"
                     /pseudo
     CDS             5578..6194
                     /locus_tag="U4B29_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6196..6714
                     /locus_tag="U4B29_002642"
     CDS             6196..6714
                     /locus_tag="U4B29_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2107543.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2061 a         1186 c         1374 g         2232 t
ORIGIN      
        1 ggttatttta taataaagat atgatgacta atttagctca actattccag cgtcagctga
       61 agatattgca attcccgatt ccttaatcat ttatctggat gagacagaaa gtgaagttgc
      121 aacagatgtg catgtagttg aggataaaaa tgatagttta aaaattaata gttcagatac
      181 tttactaaga gtaagtccta tcttttcagc tgttatttag atgcaagtag caactaagtt
      241 aggtagtact cctgcaatgg ataggtattt gtgtgttcga tatataagtg gtcgaagtat
      301 caaggttatg tatactggat gggagataga agagttaaaa attggaggtc accgccacaa
      361 atggaattta gatttagagg taccttagat ttggtatagt tattaaacac aagatggcct
      421 ggtttgtagg gaaagtcgaa gagtattttt ccacttctgc gcccctttaa tttttctagt
      481 aatgccgtga atttgtgatt aattggaaat atgaagaaag gagagtctat gaaaacaaca
      541 ctagttctat tctataaaaa gcatccttat tttacacttc tgataaatat attgttggct
      601 agtgttatcg ggatttctgt tgaatatcta ataaataaag atttcatagg ttcttgtttt
      661 tatacggctt tatttcttgg acttcttgaa gcattttcca tttataaaaa atcaaaaaaa
      721 taaaatagat tgccgtaaga gatctttttc atttcatttt tttctgaaaa cccgttgttt
      781 ctgtcagtga agcagcgggt ttttagattt attgtcttta gacctaattg agcgcgccga
      841 gcgattgaaa tacaaaacca cttgctatgc gggtggagca ataaagggaa atagtttagc
      901 ccgtacaaag tagatgtgta aataccacat tgtattcaca ccaaagtata gaagaaaaat
      961 aatttataat caatatcgag ccaatattca ggagatcatc aagagattgt gcaaatctaa
     1021 aatataaatt tgggtatcga cacttttggg tggaaggata ctatgtgaca aaagtaggac
     1081 aaaatgaagc gaccatcaaa aagtacattc aagaacaaga aaaacatgat agtgcgttag
     1141 ataagctgag tgtacgagag taagacgatc cttttaaggg ctaaggagta gtacataaac
     1201 cttttaagag gtgggcaaaa ctggcaaagg caatatggct tgaacataaa atatgaaagc
     1261 cagcgtcttt aggcgctggc cggtgatttg agcttatagc tctagagaaa accacccttt
     1321 tgaagggtgg ttatgattaa tgactttagt cagttttgct cacaaagcgt gtggaacaaa
     1381 aaacgggtgt ttctacgggt agatatttat ttcttgaatg gaggtgtccc atacattttt
     1441 ggtgttactg gttctaattt tggtgcagcg ccttcgatct taggttcttt caaccattga
     1501 tattcgcctt cgccatccag tgctttgcca ctagcccaag aaccttcact actttggtct
     1561 ccctctgaga agttatagaa gtcataagca atgtcttttc gttctttgtc acgcgggaaa
     1621 gtacttggga ctaagattga accttctttt tcttctaatt ctttcactgc acattcccat
     1681 agattttgat gatagctatc tcttgcaagg agaacagaca gcatatcttt tacgccacga
     1741 tcatcgatca tttcgtaaat acgtgctact tgaagtcttc cttgtgattc ggcatttaaa
     1801 ttagctctaa agtctgctaa caagttgcca ctcgcaatga tataggcacc gctccatgga
     1861 ttgcccacgc tgtcagcagg acgaggtcct aagccagcta cgatcgcgtg ctgtggattc
     1921 attccagaga taatggcacc gattgttgga tctttttgca tcgctgcttc ttgatcttca
     1981 acaggcgcac cctctaataa acgagcaacc attgtcgcca acatttccac gtggccgatt
     2041 tcttctgttg caatgtccat cagcatatct ttatattttt cttctcctcg gcagctccag
     2101 ccttggaaca ggtactgcat agctacactc atctcgccat attggccgcc gatcagctcc
     2161 tgcagatatt tcgcgattaa tggattcgga cgatctggtt ttgcttcaaa ctgcaattct
     2221 ttctggtgtc taaacatcaa aattcctcct aatctatttt taatacattc tcattctgct
     2281 acgaaagatt aaaaaattca aagaaagcgc tcgcgaatta tataaagtat tggtatcatt
     2341 aataattttt ttagaatcat accttataaa ttgttatttt cagaacaaaa tgatatcgtt
     2401 atattaaaac cggcatcggt tacattaaaa tttggaggta aaatatgaaa aaattattgt
     2461 ttacagggtt gctggcaagt tcagcattgt ttttattagg aggatgcgga tcaagtaatg
     2521 caagcgatgc cagttcaagt gcagcaaatt cttctgaaaa aacaagtgat ggaagaaaaa
     2581 atattgattt ctggtccttt tggggatccg gtgcacgaaa agaagctatc gaagaaatca
     2641 ttgaggattt caatcaatca caaaacgaga tccatgtaaa ttataagtat cagccatggg
     2701 gtgacatctg gactaaatct ttatctgcaa taacagctgg aaatgcaccc gatgttattg
     2761 tacaggacat aaattccgtt gcccaaagag cagaagcaca gcaagcaact aatctctcca
     2821 aatatgtaga aaaagacact tctaaggatt tttatccgca gctttgggat acagtagaat
     2881 atgaaggtga tccttatgcg atcccattca atacagatac acaagtcatt ttttataata
     2941 aaaagatatt taaagaagct ggaatcagtg aacaagatct accgacaact tgggatgaat
     3001 tagaagaaac agcacgtaaa ctagataaaa aagaaggaaa agaatttaca cgcatcggtt
     3061 tttatccatt atggaattta ggggctgatg tatgggcgct aaacgcagat aacgggacta
     3121 gttggtttga caaagatgaa aaagtcaaga ttgatactaa aaataaagtg gaagccttgg
     3181 agtggatcca agactggcaa gattattatg ggaaaaatac gatcaataaa ttagaagccg
     3241 aatttgggtc tggtgtatct gatccattta tttccggatt agtagccatg cgtgcccaaa
     3301 atatcaatta ttactctagt ttgatggaaa atgcaccgga tgattttgat tttggcgtga
     3361 tgcagctccc agaaaaagaa gaaggttcag gccattggtc gtggggtggc ggattcgtct
     3421 tggaagtacc atacggagca aaagatcctg aggcctctta tgaattcatc aaatatcttt
     3481 cttctccaga agtacaagaa aaattcggcg aaaaaagttt tgatatcatg gcgagcaaga
     3541 cagccaatga gaatttagtg aataatgaca acctaacgga taaaggaaaa atgatctatc
     3601 aaatggcgga cgaaaacttt aaaaatacgg tgatcacacc agttccttta gatgctccgg
     3661 attacacgac attagtgaat gaacagattg accagattct tttaggaaca aaatcaccaa
     3721 aagaaggatt ggcagatgca caaaaagcag tagaaaactt agtcaagcaa aatcaataat
     3781 ccatttttga ctgtctaggt acagaggagg caagaaaatt tgaagcgttt atctatccga
     3841 aagaaaaaag aagcaagatg ggcctattta tttatctcgc cttttattat cgggttcttg
     3901 gtatttatgc tagggcctat gcttttttca gttattggta gtttcaccga ttataatttg
     3961 acatccaaga tgaatttcat tggtctagca aatttcaaaa gaatgttcgt tcacgatgat
     4021 ttattttgga aatcgttata caacaccgtt tatttcgtca tcttcaatgt accgctgacg
     4081 acgattttca gtgtgttttt agccacagta ctcaatcaaa aaatcaaagg aatcagtgta
     4141 ttccgtacag ctttctattt accggcagtc ttatcaggag tagcagtcta catcttatgg
     4201 atgcagctgc tttctccatc tgcaggattg atcaacacat ttctgggctg gttccatatc
     4261 cctggaccag cttggctgtt tgatccacaa tggacgaaac ctgcgttgat tttgatgaaa
     4321 atatggagtt ctggaggagc aatgctactt tacttagcta cattacaaaa tgttcctaga
     4381 tctttatatg aatcagcaga aatcgatgga gcaggcgttt ggggaaagtt caaaagtatt
     4441 actttgccat taatcactcc tattattttt tacgatgtga tcacctctac gatcggttct
     4501 ttccaaattt ttcaagaagc ttatgtcatg acgaaaaatg gaacaggcgg acctgcaaat
     4561 tctttgcttt tttataatct gcatatgtgg aacaaggcgt tcgtagcttt tgatatgggt
     4621 tatgcgatgg ctatgtcaat gatcctgttt ctgatcgtgt tggttctgac gtttgtcaat
     4681 ttgaagcttg cacctaagtg ggtctattat tctggaggtg acggcaaatg atggattatt
     4741 tcaaaaagac cagtgtgaat cggcaaacaa aaaaatacta agttatgtcc tcatgacagt
     4801 aataggaatc attttgatca ttccattgct ttggatggtg tttacttcat tgaaaccaat
     4861 ggaagaaatc gttcgttatc ctccgacctt tttcccagaa aaaatcgtat gggaaaacta
     4921 tctggatacg attgccgcct ttcctttttg gcgctatgca agaaatacat tattcatcac
     4981 tgtgctagta gtgatcggta atgtcctttc aaattctttt attgcttatg gatttgcgaa
     5041 gcttgatttt cctgggaaaa agctgatgtt tgctttggtt ctttctacga tgatgatccc
     5101 ggggtttgta acgatgatcc ctcaatatgt attattctcc aagatcggct gggtaggtac
     5161 ttatttgccc ctgatcgttc catcgttctt cggcaatgcg ttcaatatat ttctgatgcg
     5221 tcaattttac ttatcgatca ataatgaatt gatcgaagct gctgaaatcg acggagcgaa
     5281 tcatttgtat atttggagtc atttgatgct tccattgacg aaacctgcct tgattaccat
     5341 tgcgatcaat tccttcaacg cagcttggaa tgatttctta ggtcctctgt tatatatcca
     5401 agatcaagaa aaatacacgt tacagattgg cttgcaagtt tttcagaacc aagcaacgac
     5461 acaatggaat tatctgatgg caggagcaac acttgtcttg atcccgacga ttttgctgtt
     5521 cttttttgca caacgttatt tcattgaagg gatggatttg acaggaggtt caaaaggatg
     5581 atgaatcgat cgattggtaa aaaagccaat gattatttct tagaaatcat tgaatggatt
     5641 ccacgtctta tccatttaca gctactatgg ttcctgtgcg tcttgcctgt tttcacatta
     5701 ggaactgcaa gtcgaacagt tctgtatatg atttatcatt acaaaaaaat gaagagaaaa
     5761 agctcggttc actattctgg aaaaagtttc gtgagaactt ctctctttat ggaaagcaag
     5821 atagtttagc aagtgtttat tttcttcttc tattgatcga ttatcagatt ttccgctatt
     5881 ggggtggggg aattggctat acattgatgt atactacgtt gtttttgctt ctattaagcg
     5941 cgcttctctt tagttacaaa atcttgcttc aactggaaca aaaagaagct tcttggatta
     6001 ctgcattttt cttgtttttt aatgatttca aaagcgcgct tttctatttg gcaggcacgt
     6061 tgatccttct tgtcgcattc tggtttgcag gaccaatcta ttttgcttta ttaggtttca
     6121 gtttcttgtt ttattttcaa gtcctgcttt ttatccatcg tacgcaagaa aaggttctta
     6181 aaaaggagga ataacatgtt gcgtttgatt gctaaaatcg atgatagtat tatcaacaag
     6241 ttcgccaagc aacggaaaac ggatagtcga aaaatcaatt ttttgaaatt tatcagtaat
     6301 atctctaatg gtggttttat ttggttgctc cttgctgctg ttttagtctt gatacctgat
     6361 actagagaat atggtctagc cattcttatc ggcagtttga tctatttttt tgttggtttg
     6421 ctttttatca aaaaaacgac aagacggaaa agaccttatg aaaaaaatcg tcggcttgtc
     6481 ctttacacaa gtaaaccgaa gacttcttct tttccttcag gacatacagc gctagccttt
     6541 ttatcagtag ctgcgttgtt cgaattaagt ccagttgcag gaatattagc ctttttattt
     6601 gcttgtttga tcgcttattc gaggattttt ttacttttac attatcctac tgacatttta
     6661 ggaggagcta ttttaggaag tgttattggc atcctatgcg ctgccgttat ctgaattaat
     6721 tgtacgaata aagcatgagt gcgtaaatgc gcatttgtgc tttatttttt tgtttcgagg
     6781 aaagcgtagt tgtttattca tttgtcttaa taacttaagt tttgttaaat gagaacgtta
     6841 taaaatttta taa
//
LOCUS       DASJTM010000126         6707 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJTM010000126 DASJTM010000000
VERSION     DASJTM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.128"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..846
                     /locus_tag="U4B29_002643"
     CDS             46..846
                     /locus_tag="U4B29_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107544.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            934..1740
                     /locus_tag="U4B29_002644"
     CDS             934..1740
                     /locus_tag="U4B29_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107545.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            1972..2841
                     /locus_tag="U4B29_002645"
     CDS             1972..2841
                     /locus_tag="U4B29_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2107546.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            2861..3208
                     /locus_tag="U4B29_002646"
     CDS             2861..3208
                     /locus_tag="U4B29_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107547.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            3221..4483
                     /locus_tag="U4B29_002647"
     CDS             3221..4483
                     /locus_tag="U4B29_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107548.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            4563..5939
                     /locus_tag="U4B29_002648"
     CDS             4563..5939
                     /locus_tag="U4B29_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2107549.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            5936..6565
                     /locus_tag="U4B29_002649"
     CDS             5936..6565
                     /locus_tag="U4B29_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107550.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
BASE COUNT         2583 a          904 c         1374 g         1846 t
ORIGIN      
        1 gaatgaaaaa caagtcatcg taaaatatta ataggaggtg tagtaatggt gggaatatca
       61 aaaacagctt tagactcatt agctaaacta tttagtagca ggtttgatgg tgcagaattt
      121 gttgaatatc taagccaaaa cgaaagtgga aaaactctga tcgaaacaat gaaaaaagac
      181 tatttgaatg aaaatgactt tccgacatgg acgaatattg aagatgctac atgggatgta
      241 gtatgtacga tatattctcc ttgggtaacg attgctgatt ttgttttaga taaaattgga
      301 ggggatacat accctgatgc caaatctctt gctacaagtg actttaaaac gttctatggt
      361 tatctaatag gtctaatagg caatggaata gatttgaaag ttacgccaaa acaaacacct
      421 gaagaaattg aaaaagaaaa aggagaggtt caaatggctt atgcaaatga cagagaaaaa
      481 gtaaaattat tggaaggtaa attatctgaa gaagaacttg gaaaaatggt gcaagaaatc
      541 aatgaagaca gcaactattt tcctgaattg aaggtatatc aaaatgacga agatttcttt
      601 aatgaaatgt ttagtaatag tccatgggaa gcagtgagta aaacaaattt aggagaatgg
      661 aactttcaag atgatctagt tgcatttagc aatgatgggt atttacgatc atattcagaa
      721 agtgagtata gagctgaatt aaaaacgtat gaaacggata ttctgatgag ctatttaaat
      781 ctattagaga gtggtcaggg agagttaaga gataaagttg aagaactatt aaaagaagag
      841 ctatagggat tatttcaaca taaacacaag cagaaaacta ctaaacaaaa cgtaccatgc
      901 aaaaagtcgg ttaaaccggc tttttgctct attatgagag gagatcaaac catgagtgca
      961 acaaccgata tggctttaga agcagtaata aaagataata caggaaatgt cgaaaacgta
     1021 gaagacttta gcgatattgc aatcaatctt tcagcatgga tttcagctgt aggagaagaa
     1081 aatatcgaat ttgagatcaa gaatatggat gacttaaaag caaaatttcc ttttatcgca
     1141 tcgaaactaa gtcaagttgc cgaggataaa tcgtggaatg aattagttag tttttccgaa
     1201 aaaatttatg atattacgca agaagatgag tgtgtttcat gggaaatgaa taatcaatac
     1261 aagaaaattg acgctttcgt caaatcacgt atagaaggaa cagcttttga gtttgaaggt
     1321 atagataccg cagataattg gttaaataaa gaatccttta ttaaaagtat agcggatgat
     1381 cttgaaaaac aatggtctaa ggaagactat gcagagtacc taaaaacaga aagagataca
     1441 gaggtgaaag aaaaactcac tgaggtatgt ttggatgaac atattgatcc caatatcgta
     1501 aaacaagaag actatgaagt ggttattgat aaagttccta ctgattttga ggaagttgta
     1561 aagtatctag ttgaagaagc attatcgcaa tactcttcag atgaggaagt ggacgaagaa
     1621 actgttcgta ccagagtttg gcaactggtt tttgatgaca aagattataa atatttgagt
     1681 gatcaaaact cggttaattg gagtgtacga ttattaattg atcaaaatga tattgaataa
     1741 aatagagctg aaaagctcta ttttttttgt taaaaaaact agctatttta ttaaaaataa
     1801 tttggttcaa attttatttt tttaacttga taatttcttg ccatcctttt ttaaattggc
     1861 tatatatcaa ggtttatagt gggaataaat aaaaatacat ttgacaaaat ttacaatata
     1921 gtttattata tatttatctt attatgataa cacaataaaa ggaatggtgg tatgaaaaag
     1981 aagaagttta gtcgatcgtt gttaggaatt ttggttttag ttgcggttgg tcttagtggc
     2041 aagggttact ttcagaataa cagcactcag gaacatgatt ttcagatcaa aagtagtgtt
     2101 cagataggta ataaagcaga cagtgaatca aaagaatttt tagggatacg ttatgatcca
     2161 aacaaatatc caaataatat cgtaaacatt aatgatggga aatatacact gacaaaagaa
     2221 gaccatgaag cattatcaga aaaaggcagt caaacctttt gggtaaatta tgcagattta
     2281 gatcaacttg gaagagcagg agatgttgaa gcgttgatca cctatgatgc tgtaaattct
     2341 cactcatcta aaaatgtgga acgtccatct ttcaaaagtt ctgtccatat tgcaggagaa
     2401 tacaaggatg gagtatttga tcaagagaga gatacttggg tcggcaaaga ttcaaacaat
     2461 gaaatcactc agctagaagg ctataaaggt tatctctata acaaaagtca ttcattagca
     2521 tggtcgctag gaggagatat ggaggtacac aatttaacac ttggaacgag agcgcaaaat
     2581 gttgggaaaa atgatcagaa tggagggatg gcttactcag aaacgattgt acgtgatgca
     2641 atctataaaa ataagaatga aaaagtattg tataaagttg atccaattta taaagggaat
     2701 gaattagttc ctcgtggttc tcatgtcgaa gctttctcag ttaacgataa tggaaaaacg
     2761 gtaaacctta atgtttatgt atttaacaat caaaaaggaa tcaacatcaa ctatcaaaat
     2821 ggtacttggg taagagatta gaaattgaga aggtgaagaa ttggaaacaa taagcatcaa
     2881 tggattagat aaatcagttg caaatcgaat aaaaaaagga aatggctact atcttctgaa
     2941 gaagggtaaa tattcagttg ctagagagtt agaaacaatg agtatcgtgt atgtagctaa
     3001 gaatcatttt gacgtagaac aggctatttt tgatccagat tctgaaatag ggaaatgtgt
     3061 tgaattatca gagaaaactg ttagtacaaa aggggcgatg gtcattgaga gagaaatgtt
     3121 agacgaaatc agagtaatca ttcctaaaga tacgcagatg tgtgaggaca gcataagtga
     3181 cgatgaacca aaatcagaaa gaaaataagg agatattcag atgagcgaaa aatccattga
     3241 attaaaggaa tataagcgaa gcgatggaga aaaatattat cctgtagcca gtttttctaa
     3301 atcagtatat ctggttggac gtgcattggt agttggtgca attggcatat atattttgca
     3361 ttgggtaaca aaggttgtac aactgcttgt agatacctta gtaagtattc ttgtctcgtt
     3421 aaatgatgtt aacggaacag atgatttagg attaaaagag atgtttaaag agttactcaa
     3481 tgcgtttttt cccaattatt taagttattc atataatgta caaagctttt ttgcaaagct
     3541 acacttggta gacatctcta ttttagtatt agaagcagga acggtattct ttgttggata
     3601 tttggttact ttgatgttca ggcatcacta tggagaacaa agaccatttt ttgatgatat
     3661 ggaaagtaga aggttgaaaa gagaagctct agaagctatg gatgtgaagt atgatccaaa
     3721 caagattata ggagatgaaa cgaacaacaa aaaaagaaaa cgaaacaaaa cgcaattaga
     3781 atccgatgtg ctatcaacgc ttaaaaatat caaaatttct gttcatacaa gacgtgatct
     3841 aagtgtcaat gaagatattc gagaatatat cgtaaaaatc ccgcaaccaa aagggaagaa
     3901 agttagagaa acagttttac gacaaattca agatttaggg tcgatcctga caagagcaac
     3961 aagcggacga gccaaatttg gtgaaaaaga agatgaagtt tcaggcgatt atttcatttt
     4021 ccgagcatca gtaaatattg aaagagaacg taaggaaaga gtaagtacca aaaacaaaga
     4081 aacggaaaga gcaacatctt caatcgcagt ttcaaattca caattttctt ttccaatcaa
     4141 tctctttgcc gataattcca aaaatatcca aaaccaaacc gaaaaagcta gaaaatatgc
     4201 cgaaactcaa aagcaatctt tagaacttta tctatcttct gctgacgtac ctgcaagtct
     4261 aacagatatg cacgttggga atacatctat tgaatatttg tttactttat ctcgtaccag
     4321 aaaaattcaa ctaagctcaa tgaaagaaga aattgaaact tacttgcata tatcaggtgt
     4381 aatcgtgcgg atcaacgcag ggcgtattca aattacttta ccattaggag aaaatgatcg
     4441 tattccaatt gatgtaccat caatgatcaa tagcgtgttt tagaagacat cacaaataga
     4501 aacagaaaat acaaactacc agttcacaaa cagaaaagta ccgagaaaag tgaggaatag
     4561 aaatggctat gaaattatta gagaaagtca aaaagagagc cgaaaaagct gaatctattt
     4621 tgctagataa agaacctaat ataaagctta atggaaaatt cagtgtcaat atgttgaaca
     4681 caaaggctaa agacccaaca gatgctattt ttggaattaa gcctgatggc acaattgctc
     4741 atttttgcat cagcgaaatg ccacacatgt tggttgcagg aacgacagga tcagggaaat
     4801 cagtattact gaatgaaatt cttgttacag taatgtgtca ctcaacgcct gatgaattaa
     4861 aaataggaat tgtagaccca aaaagggttg aatttggaag atacaaaaaa ttgccgttca
     4921 tgttggctga tccaattaca gatatggatg aagcttatga tttttttgaa tatcttgtga
     4981 tcttgatgca tgaacgttac aaactgatgg aaaaagcagg gttacaaaac atcacgctat
     5041 ataacgaata tgccgaaaaa aatggattag atagatttcc ctatgtgatc ttattagttg
     5101 atgaatattc acagttggtt ggaacacata aagaagtaga aggtttgatt gttcaattag
     5161 gacaaatggc aagggcagca ggtattcatg ttatcttagc aacacaatct cctcgatcaa
     5221 cagttgtcac tggtattatc aaagccaatt ttccgtcacg tgtttgcctt atggtagcaa
     5281 gtgatttaga aagtaggatt gtattagatg aaggcggtgg agaaagtcta agtccaaagg
     5341 gagatatgct tattaagctt gttgatggaa gtatggttag agcgcaggga gcttacatca
     5401 gtaacaaaga aattgaaaca atcttcaatc accttagaaa tactatgcca gaaccagaat
     5461 atgttgatta ccgagcaatc gtagctgaaa ctcgtggtga gggcggtgaa gacgaggatt
     5521 ttggtggaat tggtaaagct cctgtaagaa aatctgctaa aagaaatttg aacacacctt
     5581 tagcaaatcc atcatctaaa gagccatcaa ttgaaaagaa gagtgcgttt gctattaagc
     5641 ttgaaaaaaa taaagaagaa aaagctaatc agggagctaa gtcactagaa aaacttcagc
     5701 aagaaaaatt agaaaaacta gaaaaagcat tatcatcatc aacttctgaa actcaatctt
     5761 caaagggtat agataatgga caatcaatta caactaaaaa caagcctaaa aatagtaatt
     5821 caaatctctc ggtaaatgtt gttatcacaa ctgataaatc tgaaaaaaaa caagtgaacg
     5881 gaactacttc acccatcatg caaaaaatga taaaggcaaa aaaacaggtg cagaaatgat
     5941 tatcattagt tcctttttga gatggctaac accatataaa tatacagatg aaatagttga
     6001 gaagcgagcg aagaaaaaag ttaaaaaaac gatgttaact cctaaagaaa tgctagatcg
     6061 tggaatattg ggagtgaagg ttttagaagt aaaccaaccg tttgaattgg ataatgcgag
     6121 agataggttg cttagagctg aagttgatgt cgcatacttt tatcatgata aaagcaagca
     6181 tcaaatagct atcaaattca atccaaaatc ggcatggcga taccaagcaa gagatgacct
     6241 aaagcgtagg aagctgaaac cgatgctcta tcctgaagga aaagaggcat ttgatagaac
     6301 ccatattgtt cccattggat ttcatggtac agagtcagat aaccgcctat tggtagggtg
     6361 gaacagctca caaaatagag ttcagatcaa taaatttgag aagaaggtaa gaaaatttaa
     6421 cgatcggaaa ataatacttt ggtttgccaa tattcagaag caaccagatg ggacagcaaa
     6481 gtgggaagcg gtcatatttg atgaagatgg caaaaaagta atgtctgaag tatggcatga
     6541 taaagctaaa tttgtttggc agtagaggtg aaaattgtgt taactaacct aaaactatat
     6601 agagagggat ttaacgaaaa agtaagtatc ggtgaatcta ttgaatattt aggaaagaag
     6661 tacattatta ttcgtattct tgaaattaaa aaactttatt ttagtaa
//
LOCUS       DASJTM010000127         6644 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJTM010000127 DASJTM010000000
VERSION     DASJTM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.129"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="U4B29_002650"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="U4B29_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="U4B29_002651"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="U4B29_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN2107551.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="U4B29_002652"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="U4B29_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="U4B29_002653"
     CDS             complement(2143..2373)
                     /locus_tag="U4B29_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEN2107552.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="U4B29_002654"
     CDS             complement(2603..3421)
                     /locus_tag="U4B29_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2107553.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="U4B29_002655"
     CDS             3582..4271
                     /locus_tag="U4B29_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2107554.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="U4B29_002656"
     CDS             4285..5787
                     /locus_tag="U4B29_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2107555.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="U4B29_002657"
     CDS             5800..6276
                     /locus_tag="U4B29_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2107556.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="U4B29_002658"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="U4B29_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DASJTM010000128         6463 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJTM010000128 DASJTM010000000
VERSION     DASJTM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.130"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4B29_002659"
     CDS             complement(99..1229)
                     /locus_tag="U4B29_002659"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN2107557.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U4B29_002660"
     CDS             complement(1878..3080)
                     /locus_tag="U4B29_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2107558.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U4B29_002661"
     CDS             complement(3140..3343)
                     /locus_tag="U4B29_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN2107559.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U4B29_002662"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U4B29_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U4B29_002663"
     CDS             4880..5692
                     /locus_tag="U4B29_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107560.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1948 a         1316 c         1010 g         2189 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa ata
//
LOCUS       DASJTM010000129         6384 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJTM010000129 DASJTM010000000
VERSION     DASJTM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.131"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            112..450
                     /locus_tag="U4B29_002664"
     CDS             112..450
                     /locus_tag="U4B29_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107561.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            560..781
                     /locus_tag="U4B29_002665"
     CDS             560..781
                     /locus_tag="U4B29_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107562.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            801..2957
                     /locus_tag="U4B29_002666"
     CDS             801..2957
                     /locus_tag="U4B29_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107563.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2969..3202
                     /locus_tag="U4B29_002667"
     CDS             2969..3202
                     /locus_tag="U4B29_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2107564.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3192..4058
                     /locus_tag="U4B29_002668"
     CDS             3192..4058
                     /locus_tag="U4B29_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107565.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4073..6043
                     /locus_tag="U4B29_002669"
     CDS             4073..6043
                     /locus_tag="U4B29_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107566.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            6210..>6384
                     /locus_tag="U4B29_002670"
     CDS             6210..>6384
                     /locus_tag="U4B29_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107567.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         2454 a          876 c         1249 g         1805 t
ORIGIN      
        1 gatgtaaata tcgagacgtc tttttcttag ttagatcatt gacaagtgaa caaataagat
       61 ttgtcgatga tataattaac ttattaaaaa tattaaaaag gaggttttta tatgttcgat
      121 tctattaaaa atttggttag cggaatgacg cctgatatga aaactttagt acaagttgtt
      181 ttgtatttct tggcattcgt tttttcgttg aaaccaggat tcactgcaat gaaatttttt
      241 ggtgatgcaa aatggggaga tggattaaaa tatgttgggg cgattattgc aatctttatt
      301 attcctgtaa ttacaaccgt tatgctttac acaatgggta ctaaaacagg taatgatttg
      361 aaccaaaaag ctaatatcat atcagcactt attccagttg ttggtatcta tctagtttca
      421 aaatttccaa aaaatgaact gttaggttaa aagtgaattg aacaaatttg gataatatgc
      481 cgatttgttc aatttacttt ttaccaaaaa actacgctcc aagcgtagtt tagaagtgag
      541 ggaagaaagt aaatgtaaga tggtttttga tagagaaaaa atgttagctc acgccaacga
      601 agtattaatg agcagtctga aaggtaccga actggcaaag ataatgaata tgaatgttaa
      661 ccagttctat gattatcgaa atgggagtaa aaagattgaa aaagccagac ttgaaacgct
      721 tattaaattc gaaaaagcat atgtatatat gctagataaa cagaaaagaa ctattgactg
      781 aaaaaaggag ttttacaata atggcaaaat tcaaagatat taccgaatct attaattatg
      841 tggcagaaac agttcctgta gttgcactct ctttgaagcc acaattactc gaagtgtaca
      901 aaaaagaaga aaaagaagcg aaggcaaaaa aagctaaagc aaaggtatgg aaagaattcg
      961 atgcgttgct gaccaaagat ttaccaaaga aaaattatat ggtaattgaa gatgtacaaa
     1021 agctcaacag tgagggagtt atagatgagg atgatgctat tctcgattct gagaagtatg
     1081 tcgttctatt gaagtcaagt ttgaattatg tagctgatct accagaaaaa tttaaagaga
     1141 agctattagc aggtgtccgt tttatggata gtagtggtca attattagtt attccggatg
     1201 atcctaatgt gtttgaatca gaagttgaag aagctacaga cttctatagc gcagtagagc
     1261 aattagaagt agctactaat ggattagaac gaattgctga gaccttcaac tcttctgaag
     1321 aagttgaatt tgaagccgta gcagaaaatg taccagatga aaaagaagct ctttttgtgg
     1381 atgataacaa accaaccgaa gcagaaaacc aagaagtaag catagaagaa acacaaatac
     1441 aaacagaaac ctaccaacaa ccaaaaattg atgaaaaacc cgaatcccaa agtgacaaag
     1501 aaaattatgt ttatgaagct gaaaaaatca catctgaaag tgatgtaccg aaaaacttag
     1561 atgctgattt tttagaaaaa aattttgaac agatcattgc tgacaaattt caacctgtag
     1621 ttttagatga gattaatgac cctgtcgatt taaacgagga gatgctatcc atacctgagt
     1681 tgaaaccatt gctgttttta gctaaagaga gtatgaacgc tcgcatctct attgctaata
     1741 ataaaatcaa cagtgaatta aataagacga aggagcaatt gatcctcaaa ggaaagcaaa
     1801 tgttagctga tgagcttttg cggatagagg aagcaacctc attaaatggt caaaatcagt
     1861 ttacaaatac actatcggtt gctaaagaga gctacgaagc agaagcatct aaaattgatg
     1921 atttagtgat ggaaaaatcc aatatcattc gtaaagatta tgatatggcg aaacaggctt
     1981 atttggatgc tgaactaaag aaactagagc aacggtatga tgctgagcat ttaccagaag
     2041 ttaaacaaca agaagatgaa tatagaaaag caactgaaga aagtcttgat caacagtacc
     2101 aagaagcaat tgaatctatt aaaaacgatg cattgaaaca aagccaagaa gcaaaagaag
     2161 ttgtggtgga acgaattatc aattcattga aaccagaaat tgaaaaatca ttaacagacg
     2221 tgcaagaaaa aactttagag attgtcgaaa aaacaaagtc agaaaatgaa gctgagttcc
     2281 atgatacaaa agagaaaaca acagatgtgg ttgtcaaata ttttgcgaac aaagaagaag
     2341 ttgaaaagca ggtagctaac aacacgaaag aattagaaac aaaacttgta gagcaacaaa
     2401 aaactatcga agaatataaa gatcgaatga aagaggtcaa agatttagca gagggttata
     2461 aattaaatag cgaaaagttg gcagaacaaa gtgaagcttt aagagaagac ttatctcgtt
     2521 cccgaagtga tctagctcaa atggcagaaa aaacgaaatc attagcagtt gctacagaaa
     2581 atcaatcaag ttcaaaacgt gattttacaa gtaaaaagat ggctactatc atttctgtcg
     2641 ctgcaatggc ttgcggaacg atcttgggat cgtcctactt tattagtcat aataatcaac
     2701 aagcacaggc tcaaactcaa acatctattt cgagagtgga aaattcgatt gggacaaata
     2761 cgaactctgc ttcaacaaat agtagctatg aagcaacacc aactacagag ttcaaagttg
     2821 ggaaagatgt tgtcatcagt attgatggga aaacaactaa ggctgaagtg atttctattg
     2881 atggagaaaa agcagttgtg aaaactccag acggaaatca gtacaaaatt caagctcact
     2941 tatctgataa taactaagga gggctattat gcgagaagaa aaaatagttt atccagtaat
     3001 tcaaaacaat caagaaaaca tgacctcttc tcagcactta caggaagcac agctcgtgta
     3061 tcaggtaaat gaactaccac aaacgaacga gacaatgagc aagtcaatat ctttaattgg
     3121 cttgcttatt gttgcaatag tgataactgt aggtgtttac cgtaagttga acgtgaaagg
     3181 aagtatttct gatggcagat aactcaaact atggatttaa acataactta catcaaaaac
     3241 cttctgaaac aattgtcaga gttgaatggc ttgaaaagca tgtcaatagc acctattctg
     3301 ttacagcaaa aagattaatt gtatcagcta tagttgttgc agtcgttgtt cttggatcaa
     3361 ttgtttactt agtttctgat acgattaaac tgaaatttat ttttgttcca acgttattaa
     3421 tgataattgg atttcttatt ctaattagcc agtatatgtt tattgatcca tctagtggtt
     3481 cattgatttc tggttctatt agactgttca aatcagtagt taattcagca agagtatcta
     3541 tggggaaaaa gaaaccattt cgaggcacag agattttaaa agtgaaacaa gatggaacaa
     3601 tcgtatatcg agatgggagt gtagcattaa tgttcaatct agatggggct acatcactaa
     3661 ccgcttttcc agaagaagtt atggctcagg aatcgaaatc aatacaatat caaaatacca
     3721 gagatcgttc cttagctgaa atcaaaatca cttcatctca acctcagaat acggaaagac
     3781 aaactcgaaa tatgaaaata caaaaacgaa atgcaaggag taacaaagca gtctttgaca
     3841 taatagatca gcaagaacga tttgtatcag aatatattga tggtgtgaaa acaacagtaa
     3901 tccaatatct tatcttgaaa tctcaatcta agatggtatt ggatgaaggt gtggaacgtt
     3961 tagaaaacta tacgagtaga ggactttatt atagtgtcac tcagttgtca gctaaacaaa
     4021 caaaagaaat attatctgat atttatgcac tgaaatagag gtgagagatt aaatggaaat
     4081 tatgggtaga tcacaactat taaatttcaa aaaaaagaga aaaactaaaa aagaacaaaa
     4141 aactagaaat tcaaaggtag tgaataatac ctttgttagt gatttaacat ctttagttgg
     4201 ttatcaattt acaccatctt acattaggac aggtaagcgg tggggaacaa ttgtgaaatt
     4261 agtgaatact tatggaatga atagaaatta tggatttggt tggatggtta aagctattcc
     4321 tgaagtgaga gtagaagggg taaaagccta tctaattgaa gcagataagc cagtgtcaca
     4381 aaaagatcaa caagaaataa tgcacgagaa tgttggggga tcaacgaata gttatgaaaa
     4441 taatttagaa ggaaaaaatt cagatgctga aattagcaaa actgttatgc ggattatgga
     4501 tttgaataca gcatctttac tagacagcat ggcaaataaa attattgatt ggaagctaga
     4561 aatcctatta gtatccgatt ctcctaataa aatcagcgaa cagctaatta aactccaacg
     4621 gttgtatgat gaaaaaatgt atggattaaa gcttatgtca tatggaggag atcaacgtga
     4681 tctgtttgat agacttttaa gtgaaccaat cgggaatgtc tatgagtata cgtctatgag
     4741 ttcggatttt gcaggaaatg atcatgcaat tcgcaaaggt ttagatgatg cgaatggatg
     4801 ggctatcggc actatgtcta ctagctacgc aagtggtgta gcttttatgg cactggatga
     4861 ttcttttaca agtaaagttt tagtggcatc acacaaaaac gctacaatta aaggctatga
     4921 tgaaaaaatc tcagcatcat cactttgggg gcaattgatt gctaataatg caatggcaaa
     4981 tcataaacga acttttcata ttgtcttaaa taaatttgat tactttggtg atccaactgg
     5041 tagaacattt gttgccaaac cttcgttaaa cagtgtaata gaaaaggtta atttggctga
     5101 agaaggtctt aactctttgg agctttttgg taatcaaaag gacattggtg atttatatag
     5161 acggaatctc gataaactaa ctcagatttt ttatttgtta gcaggcagaa cattagattc
     5221 tgcaactaga gttttattga aaaaagaatt aggtaacttt tatataagtc agaaactttg
     5281 ggatattaat tatgaagaac gttcaaaact tttggtgaac agtagtgata caatgccagt
     5341 tcttggggat tttattagct atttgactaa tgctgaaaca aaagccaaaa aatctggtac
     5401 tgaaaaagaa gtcgatcgta ttattgtatt aaaagacaca ttgagaacag cattagacag
     5461 cttctctagg atgtttaatc aaagaacgac actgaaagaa atcggtaaaa gtgacaaatt
     5521 acagttttac tataacttag gtgatctaag agcagaaacc aatgttatgg aagctcaatt
     5581 tttaaatacc tttgatttca tcacatcaca agcaaaaaaa gatgatattg ttatgattca
     5641 cggcttcgat aagttatcta ttgaaaccgt tgggctaatt aaaaaaagat taacaatgct
     5701 tgaagaagat ggaattcgtc ttgcctttct atttgataca atcggttcag gagaagacaa
     5761 tgaatacaaa gaagatattg aaacagctga tatattcaat acagatggaa aaatttacca
     5821 aagttttgaa agtgatttta attacactat cattggaaag atgaccagtg gagagttaat
     5881 gaagtatgaa aaaaaagttg ctcctgctag aaaaggagaa gatgatcaaa aactttctgt
     5941 tgatttgaga cagtatttga caacaggaag aagtaataaa tttcagatca ggagaccagt
     6001 ggatctgact aataattttg ttattggaga gtttattctt taagttttat ctttgatttt
     6061 gagtgtataa ggaaaggata agaaaaaaca tgtatatttg agactaaaaa cagtcttaga
     6121 gaacatggta cgttttgtga aaagagaact atagaaattt cataaagtga aaccttactg
     6181 agtgaaataa ttatagtgga ggttaaataa tgaaaccaaa cataaaaatt ttagatcgaa
     6241 tatttctagg tagagacaca gaagtaatcc taattcaaca tgaagaagga tttgaagtta
     6301 gtataggtat tcagaaactt caaaaacctc attattgtaa tcagttgtat aaaaacttta
     6361 ctgatgaaga aaaagcgaga gttt
//
LOCUS       DASJTM010000130         6363 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJTM010000130 DASJTM010000000
VERSION     DASJTM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.132"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(175..363)
                     /locus_tag="U4B29_002671"
     CDS             complement(175..363)
                     /locus_tag="U4B29_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN2107568.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            633..995
                     /locus_tag="U4B29_002672"
     CDS             633..995
                     /locus_tag="U4B29_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2107569.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1047..2729
                     /locus_tag="U4B29_002673"
     CDS             1047..2729
                     /locus_tag="U4B29_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN2107570.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2846..4882
                     /gene="recG"
                     /locus_tag="U4B29_002674"
     CDS             2846..4882
                     /gene="recG"
                     /locus_tag="U4B29_002674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN2107571.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4931..5932
                     /gene="plsX"
                     /locus_tag="U4B29_002675"
     CDS             4931..5932
                     /gene="plsX"
                     /locus_tag="U4B29_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN2107572.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6054..6296
                     /gene="acpP"
                     /locus_tag="U4B29_002676"
     CDS             6054..6296
                     /gene="acpP"
                     /locus_tag="U4B29_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2107573.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2135 a         1102 c         1436 g         1690 t
ORIGIN      
        1 cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac ttcataattt
       61 gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa
      121 atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac
      181 gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc
      241 gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat
      301 agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc
      361 catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga
      421 ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca attgacaatt
      481 gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta
      541 ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat
      601 gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg
      661 gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg
      721 tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt
      781 tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg
      841 tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc
      901 aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg
      961 tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc
     1021 agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc
     1081 aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat
     1141 taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta
     1201 gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc
     1261 tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt
     1321 ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg
     1381 cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa
     1441 cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg
     1501 atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa
     1561 ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac
     1621 tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa
     1681 catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg
     1741 tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa
     1801 aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt
     1861 tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg
     1921 tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca
     1981 aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag
     2041 tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg
     2101 ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg
     2161 gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag
     2221 ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag
     2281 aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag
     2341 ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa
     2401 tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg
     2461 gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta
     2521 acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag
     2581 agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg
     2641 agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg
     2701 tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt
     2761 attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt
     2821 ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc
     2881 aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga
     2941 cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat
     3001 ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg
     3061 atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa
     3121 cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc
     3181 cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc
     3241 tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac
     3301 attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct
     3361 gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat
     3421 gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga
     3481 gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa
     3541 tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt
     3601 tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc
     3661 gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc
     3721 gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga
     3781 aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac
     3841 catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact
     3901 agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga
     3961 atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg
     4021 gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat
     4081 tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact
     4141 tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt
     4201 actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct
     4261 gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg
     4321 tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga
     4381 aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac
     4441 tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga
     4501 tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc
     4561 ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat
     4621 catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc
     4681 tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt
     4741 gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa
     4801 ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca
     4861 ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg
     4921 agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat
     4981 tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg
     5041 aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac
     5101 agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc
     5161 atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc
     5221 agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat
     5281 cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga
     5341 tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat
     5401 tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt
     5461 aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct
     5521 tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg
     5581 tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga
     5641 aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa
     5701 aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga
     5761 ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca
     5821 tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga
     5881 gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga
     5941 tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa
     6001 attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc
     6061 gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg
     6121 ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta
     6181 tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg
     6241 aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc
     6301 aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatccttct ttttttaatc
     6361 ttt
//
LOCUS       DASJTM010000131         6157 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJTM010000131 DASJTM010000000
VERSION     DASJTM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.133"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(368..853)
                     /locus_tag="U4B29_002677"
     CDS             complement(368..853)
                     /locus_tag="U4B29_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107574.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(992..1207)
                     /locus_tag="U4B29_002678"
     CDS             complement(992..1207)
                     /locus_tag="U4B29_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN2107575.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1431..2231
                     /locus_tag="U4B29_002679"
     CDS             1431..2231
                     /locus_tag="U4B29_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2107576.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2250..4118
                     /locus_tag="U4B29_002680"
     CDS             2250..4118
                     /locus_tag="U4B29_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2107577.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4111..4602
                     /locus_tag="U4B29_002681"
     CDS             4111..4602
                     /locus_tag="U4B29_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN2107578.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4590..5144
                     /gene="srlA"
                     /locus_tag="U4B29_002682"
     CDS             4590..5144
                     /gene="srlA"
                     /locus_tag="U4B29_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN2107579.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5162..6157
                     /locus_tag="U4B29_002683"
     CDS             5162..6157
                     /locus_tag="U4B29_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN2107580.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2177 a          976 c         1229 g         1775 t
ORIGIN      
        1 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
       61 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
      121 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
      181 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
      241 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
      301 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
      361 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
      421 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
      481 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
      541 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
      601 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
      661 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
      721 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
      781 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
      841 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
      901 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
      961 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     1021 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     1081 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     1141 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
     1201 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     1261 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     1321 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     1381 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     1441 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     1501 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     1561 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     1621 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     1681 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     1741 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     1801 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     1861 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     1921 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     1981 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     2041 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     2101 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     2161 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     2221 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     2281 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     2341 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     2401 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     2461 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     2521 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     2581 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     2641 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     2701 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     2761 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     2821 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     2881 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     2941 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     3001 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     3061 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     3121 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     3181 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     3241 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     3301 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     3361 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     3421 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     3481 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     3541 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     3601 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     3661 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     3721 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     3781 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     3841 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     3901 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     3961 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     4021 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     4081 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     4141 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     4201 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     4261 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     4321 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     4381 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     4441 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     4501 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     4561 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     4621 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     4681 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     4741 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     4801 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     4861 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     4921 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     4981 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     5041 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     5101 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     5161 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     5221 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     5281 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     5341 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     5401 aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc
     5461 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     5521 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     5581 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     5641 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     5701 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     5761 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     5821 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     5881 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     5941 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     6001 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     6061 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     6121 cattgcttgg gtagcaagtt ttggattata tagctga
//
LOCUS       DASJTM010000132         6149 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJTM010000132 DASJTM010000000
VERSION     DASJTM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.134"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(270..833)
                     /locus_tag="U4B29_002684"
     CDS             complement(270..833)
                     /locus_tag="U4B29_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107581.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(820..1347)
                     /locus_tag="U4B29_002685"
     CDS             complement(820..1347)
                     /locus_tag="U4B29_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107582.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(1370..2707)
                     /locus_tag="U4B29_002686"
     CDS             complement(1370..2707)
                     /locus_tag="U4B29_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2107583.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2827..3306
                     /locus_tag="U4B29_002687"
     CDS             2827..3306
                     /locus_tag="U4B29_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107584.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(3421..3653)
                     /locus_tag="U4B29_002688"
                     /pseudo
     CDS             complement(3421..3653)
                     /locus_tag="U4B29_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3656..3973)
                     /locus_tag="U4B29_002689"
     CDS             complement(3656..3973)
                     /locus_tag="U4B29_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107585.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(4084..4266)
                     /locus_tag="U4B29_002690"
     CDS             complement(4084..4266)
                     /locus_tag="U4B29_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107586.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(4303..5103)
                     /locus_tag="U4B29_002691"
     CDS             complement(4303..5103)
                     /locus_tag="U4B29_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107587.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(5240..5659)
                     /locus_tag="U4B29_002692"
                     /pseudo
     CDS             complement(5240..5659)
                     /locus_tag="U4B29_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5655..5963)
                     /locus_tag="U4B29_002693"
     CDS             complement(5655..5963)
                     /locus_tag="U4B29_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107588.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
BASE COUNT         1833 a         1287 c          775 g         2254 t
ORIGIN      
        1 ccatatatat tgtctttaat agttttattt gactttttaa gaaagtattt acttttgact
       61 ttttttacgt tttacaacat atacgtattg taatgttcag ctcaatttat tataatctaa
      121 atgaattgat taataaaaat tctgaggttc gggtttttta tcaattcttt tctgtttgtg
      181 tttgtgatga aacaatcctt tttcggtagg ttttgttatc atcgtcagca caaagagcta
      241 acgttggtac cgttagctct ttttttattt tactctgctc ttacatagct atctaaatcc
      301 tccaaataaa acaattgatt ttctcctatg cttgttactc cacgccaaat tgttgttgtt
      361 gctgttgttg gagtatttct tgtctgtgta atcaattcaa ccattttacc tttttgattt
      421 gttgtatagt aatcagcagt atagctagat attggactat taccagtgcc taccatcgtg
      481 ccagaaatat caacaggact agactttact atctgaggaa attctttttg tatttccttc
      541 atattcttcg tatatttttc ccatgaattc cagttgtaaa ataaattaaa gaatttccta
      601 ctttgttcct taatttcagc atttcccaat tctttgtatt gagcttcctg catagctttc
      661 actttctggt tattcttgac tacttcgatt tgtttacttt taaggtcttc caattctttg
      721 acattattat cataagcatt tttcttttga aatagtacca cacaacaaag gatcacaatt
      781 atggaaagta ttccgatcgc ctttttagca gtagtgattt catgattttg cataaaaagc
      841 acccccagtc actttcttat cattatttag tgtgatggtt aatgtggtat catacacttt
      901 ttttccattg tattgaactt caaaatcggt caatgcttca tacaacccac tactagatgt
      961 cttaattcca aagtttgtta gcttaaaagt aaaaggctta tagtactcga ttttttcttt
     1021 ctcactactc ttatcagtaa ttggtggttt acctgttaca ataaatagtt ttctcaaaaa
     1081 ttcttcacct gattgatctg caaacttatt ttgaactgat ccattcaaca tttcttgaat
     1141 atcattttct aaatctttcc gtaatccatc cacctcattt ggtttaacct ctttaaagaa
     1201 tgtcagctta tcttcaatat gcacactgtg tttttcagct tctatgtcct gattctgctt
     1261 attgaagaat aggattccaa caagcagtgc taataacata ccagaaagca aagaaatgat
     1321 aattgtatct ttcactttac tattcatcta tatcgtcctt cattccaatt tatttttcga
     1381 tttcaaattt agcttttcct acaccgctaa agaaatgcac attatctgat ccgtccactc
     1441 catcaatcaa cgttaccgaa actccccttg ttggagcttg ggttggtgga acattaaagt
     1501 tagcacatag aaatgatcca tcttcaaaca ccgctataac tacaccagta tgccctgcat
     1561 ctccaacagc ccataaccat ccatccgaag cactaaaccc ataacctacg attggtttgt
     1621 tcgttatttt tgctcctgcc gatttgtagc tttcccatac ataaccacca tttccagtag
     1681 tcggttgatt cccatcgtat aattggctca tataagccca cgtcaactct gtacactgac
     1741 cttgatatat tccgttaaaa ggtgaagttg accaatctgg ttctttgaat tttggtagcg
     1801 tgatcgcttt cccatatttt ttcaaagctt cttcataagt catattcgct attacttgac
     1861 cttttttacc gttaattttt ccttctttcc caattgatcc agaaccagaa ttagcgtcat
     1921 ccgtagttgc acaggcatct tctactgttc ctgatgctga acttgatgaa ttattcgatc
     1981 catctccggt acttgaacct ccggatattt tattgatctt actttcatct tttgcaccgt
     2041 ccataccctt gcttttcatg tacttccaga tttcttttgc attttcacca cgtacagcta
     2101 ttacagctga ttcggaatca gctgaaattt cccagttttt atgaaaagtt actgcatttt
     2161 ctacgacatc atcagaagca tttaaagcat aatcttttaa tattcctacg aatcccccat
     2221 cacctttgaa cataaagtcc aattgggctt ctgtagtcca ccaagaaccg ccattatttt
     2281 tcttagacca atcaactaat gctgtatgtc gcccagctgt ccattgacca tagccaatac
     2341 ccatcagacc atcagtaaat tgtttgatga agtcaacttc tctctgtcca tctccgtaat
     2401 acccttctac tgctcgagga tcaatacctg attcttgttt ccagttacct aaaatacctg
     2461 caatactagc acctgataag ccatattctc cattcatgta tttgtaaata tcttcaatat
     2521 ttttggctac atcacctgtt acagcgccac ttccagtatc gtttccatcg ctatctgttc
     2581 ctgacccact accttcagta gtacagttaa attgtgcatc ataagtagct atcccatcag
     2641 catgtatcac ttgcggattt attgtgatta cactcattgc aatccctgta aattgcacca
     2701 ttaacataag caatgtggca atctttctct tagatttttt caataactac gcccccaaaa
     2761 agtgtacttt tttatgttat tatgataata taataacata aattaataga aaggaaaagc
     2821 taataaatga ctgataaaac aagtgattta cctaaactag ctccaaaaac atctcgagta
     2881 aagagcaaaa aagatggaaa aacaaaaaat gctgtaaaga aaaagtcagt aaaagaaaca
     2941 tcctcgttac tacttgatat tccaatgagt tcttttgatg acaatacgga cgaaacttct
     3001 gttaaaattg caactcacgt tggaatgaac aatcaaaatg attcttttta caacttgtta
     3061 aacagcctaa gagagttgga agataaaaaa aatcaggtaa tcgcatcaaa agctcctgat
     3121 ctcacaaccg atttggatgc tgaaattatc caaatcaaac aagatgagcg atcagttaaa
     3181 tcgctactga aaacctactt tgatggtttt gataaagaac tagctcctta tagccaagca
     3241 atcctaacta aaccaataca agctccacat atttcaattt ccctacaaaa aaagagtgat
     3301 gattaaacaa tcattactct tttttagatt tctggtaagc gctgatcgca aaaaccatca
     3361 acaatattga ttcaaaaaat atgttcattc gtgtctcttt tttccattat agatttccca
     3421 ctattccatt aaatcgaaag tcacaccgat tactacaaag attaacgtga ccacattcaa
     3481 aaacattaaa agatatgctg ataatctcat cattttttta gttcacttac tggtaatcta
     3541 aacatttgaa cgattgcact ggaaatacca taattagcaa tgacaaacaa aaaatacgca
     3601 ataatttcac ttacacctag atagatcagt aaaataaata agtcacttaa cattgttagt
     3661 ttcctcccag tatatgattt tcatttttgg gaagtctttt ataatccaat tcctcaaaaa
     3721 aaggaaccat agtgtcacta atataggttt tagtttcagg taccgccaaa attccccaat
     3781 caagaatttc ttcccatgat acatcatcaa attctaaaat attatgatca actgacctat
     3841 taccaagttc atatccacca aagatcacca tattatcaat catgtataga atttcatcag
     3901 gcgaaccatg agttaatgtt tcgtcctctt caagcagttc gtttaatctt tgtttgtatt
     3961 cctctctttt catactttta tcccttctga ctcatttttt tgaattttaa catttttaac
     4021 ccaagcatga ctatctttac atgctattaa tttttatctc cattttgata tttctgtttt
     4081 tttctactca ccaatcaacc gtaaatcctc aatattctca ccttcaagcg aatatgaata
     4141 tacttcgccg taaatatttt ctataactac gacttcttct tcaaagtcta tggactgcac
     4201 atcaaaccat tctcctccgc caaattcgtc tatgattagt acctgcaata ttttatttac
     4261 tgccatttca atttcccctt ataccattct atgtcttcca aactattttt cttctttcca
     4321 taccaaggtt tcacgtatct caaaattttc tttaaataag tgttccggta cttttataat
     4381 taactctttt cctactttta tcagatagtc tttttcacct aatgagattt gaccagtcgg
     4441 taactgtata gaaactcttt tagttttatt aaatttttta gttttcaata cagtatacgg
     4501 tctacagaat ttctctaagc gttcaaatct tttactatcg tattggaaat aagctttagg
     4561 aacagataaa gtacaaaaac tatcggtaac agcacttatg tcttttttag aaattatccc
     4621 aactttagaa cttctaagga aagttgctaa tttcttatat ccaccatgac ttgtttcaat
     4681 atagatgcta ttgtattggc tgtcgtctat ctcatttatt gtttcaacct ttttcctgac
     4741 aggtgtttca acgagttctc gaacctcttc ttcactgtac gctaatacaa ttaactcttc
     4801 cccatttacc atctttagaa catacgcttg cttctttttt cttggcttat tttgagattc
     4861 aacataacca gacatggaat ctttatactt ccaagcatgt acttttttct ctaatttctg
     4921 tatctctttt ttcagctgta gatattcaga cattttttca ttgtaacgtt cgcaatataa
     4981 acaatattca tcttttccaa aatcatgatc aagtccttct tcaatatgtt tattcaacaa
     5041 accggaaaaa tactgatcat actgcttctt tcgtaacttc agctctcgta aatcttcaga
     5101 catttcattt ttcctttcca attattggtg ccatttcaca atttgggatt gactaaacac
     5161 cataattaat ttgtaagcta acacaattac aactgttggt aattgtgtta gtctccatca
     5221 acttgtctgg tggctttttt tattgcacaa atttcatagc tctcaaaaag cgcttggctt
     5281 gaaaaggtgt tattctacga accttattcg ccaatttcca atctgataaa gcaatttgcc
     5341 attcgtgcat ttcaaaatcc ggcatgattt ctttgatata ctgtagctct ttcttttctc
     5401 tttcttttaa gaaacggctc atcgagttta acattggaac taaggatgcg taggtacttg
     5461 aataacttcc aatgcgtttc gtagtaatta cataatcaat aaaaacacta ttaagaaagg
     5521 tcaagtttat aaactctgat tcatacactc taaaatttgc agttaatcgc ttcgccttgt
     5581 caccccaata gtcacttttg ttgtaggctt tttcccctcg aatatagcac tctgtttctc
     5641 tttcgtaata atagtcatac ttttttcatc atcaaatctg agagtttttg attatctttt
     5701 tcactgtaaa ataatctact cgttaaactt ttaggataat ctttgataga atatgcatcc
     5761 caaacaattc tttgaacata ttcacgatct atattttctc tgacttcctc actccaataa
     5821 tgttgaccgt aatagcattt ttctattacg ttgttagcag aaacttcaaa ataggattgt
     5881 tccctttgta tcctcatacc aatcatcata gcgcaggaaa taactattaa taacaatcct
     5941 gtttttaatc tattattcca catgcagttt ccacatcctt atcttgataa aaatcctctt
     6001 gaccctctcc aattactgtt ttaaatataa ccaccaaaac aagatcatgc taagaaatcc
     6061 aagagtatat aagatgatac attctttgtt aattatccaa aaaactctga tttctttgag
     6121 cttttcacgc attaggaaac accttgtta
//
LOCUS       DASJTM010000133         6076 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJTM010000133 DASJTM010000000
VERSION     DASJTM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.135"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(254..541)
                     /locus_tag="U4B29_002694"
     CDS             complement(254..541)
                     /locus_tag="U4B29_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2107589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="U4B29_002695"
     CDS             complement(561..785)
                     /locus_tag="U4B29_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN2107590.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="U4B29_002696"
     CDS             complement(1240..1410)
                     /locus_tag="U4B29_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107591.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="U4B29_002697"
     CDS             complement(1425..1958)
                     /locus_tag="U4B29_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2107592.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="U4B29_002698"
     CDS             complement(1979..2923)
                     /locus_tag="U4B29_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN2107593.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="U4B29_002699"
     CDS             3560..3646
                     /locus_tag="U4B29_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2107594.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="U4B29_002700"
     CDS             complement(3767..4456)
                     /locus_tag="U4B29_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107595.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="U4B29_002701"
     CDS             complement(4701..5615)
                     /locus_tag="U4B29_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2107596.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="U4B29_002702"
     CDS             complement(5597..5977)
                     /locus_tag="U4B29_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN2107597.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1610 a         1121 c         1023 g         2322 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagg
//
LOCUS       DASJTM010000134         5901 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJTM010000134 DASJTM010000000
VERSION     DASJTM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.136"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(525..1028)
                     /locus_tag="U4B29_002703"
     CDS             complement(525..1028)
                     /locus_tag="U4B29_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2107598.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="U4B29_002704"
     CDS             1387..2412
                     /locus_tag="U4B29_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2107599.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="U4B29_002705"
     CDS             2735..3613
                     /locus_tag="U4B29_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN2107600.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="U4B29_002706"
     CDS             4089..4205
                     /locus_tag="U4B29_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2107601.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="U4B29_002707"
     CDS             5133..5471
                     /locus_tag="U4B29_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107602.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..5795
                     /locus_tag="U4B29_002708"
     CDS             5565..5795
                     /locus_tag="U4B29_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107603.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2007 a          888 c         1081 g         1925 t
ORIGIN      
        1 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5821 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     5881 cagtataaag agtatccaga g
//
LOCUS       DASJTM010000135         5866 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJTM010000135 DASJTM010000000
VERSION     DASJTM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.137"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            426..479
                     /locus_tag="U4B29_002709"
                     /pseudo
     CDS             426..479
                     /locus_tag="U4B29_002709"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            483..1082
                     /locus_tag="U4B29_002710"
     CDS             483..1082
                     /locus_tag="U4B29_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2107604.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            1098..1574
                     /locus_tag="U4B29_002711"
     CDS             1098..1574
                     /locus_tag="U4B29_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107605.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            1591..2493
                     /locus_tag="U4B29_002712"
     CDS             1591..2493
                     /locus_tag="U4B29_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107606.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            2566..2757
                     /locus_tag="U4B29_002713"
     CDS             2566..2757
                     /locus_tag="U4B29_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107607.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            2921..3508
                     /locus_tag="U4B29_002714"
     CDS             2921..3508
                     /locus_tag="U4B29_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107608.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            3508..4407
                     /locus_tag="U4B29_002715"
     CDS             3508..4407
                     /locus_tag="U4B29_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEN2107609.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            4407..5321
                     /locus_tag="U4B29_002716"
     CDS             4407..5321
                     /locus_tag="U4B29_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107610.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            5373..5588
                     /locus_tag="U4B29_002717"
     CDS             5373..5588
                     /locus_tag="U4B29_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107611.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         2238 a          749 c         1185 g         1694 t
ORIGIN      
        1 taaccctaac aaatataatg ctcttgtcca atgctatatc gaaaacaaaa ttacaatgca
       61 agaagtcgct gatgaattag gcaaatcaaa aagcctagta tcacacgact ttaagcacaa
      121 tgtttttccg aacccgattg agctgaggaa aaaatttcaa aagagagttt atcaagaaat
      181 agggaagtgg attgatgaag ggaatgcaat tgacccttta attgaaaaat atcctttttt
      241 aaaaagtaaa aataaaattc aaaggttaat tcgtcaaggc gatattcaag ctaaagaacc
      301 tgttttgaca accaatgatt tggctaaaat aaatcttcat aaacagatta ttgaagactt
      361 tgaaaaaaat ctttcaacaa ctgaaatttt aaaaaaatac aatattccga gaggaaagct
      421 aacatcgact ctttttagag aatatggaga atcggcagtt actaaagcaa aggaagtagc
      481 gaatgagaga aaaaaacaaa aatagtagtc gatacctagt agcgactgta gtttttgttt
      541 gtgtattgat tttcctgttt gattttttgc agattggaaa aagcataatt ggaaatacat
      601 tgagtgcaaa ggaaatggtt gctttaggtg gtttgtatga aggacatttg ttcccgaata
      661 tattcatttc tatcgtttct catattaact tgtaccattt aatttctaat ttattgttct
      721 tagtagttct tgaagaagca ctgaatgatc aatatgagtg gttaacacta ttgattgttt
      781 taatcatatc aggacttcta ggaagcttag ttccttttct gttacaaagc aacatcgtaa
      841 gtatggggct tagtggaggt atttttggat ggattgcttt atttgcgatt gcatacatcc
      901 gctttgatag taaggaacat ttattactaa gtgtttttat attattgata atcggcattt
      961 tactggtatc tacttttgaa tcaggcgaga caaacgtctt gtcacatgta atcggatttg
     1021 tctgtgggat gatcttggga tttattctaa agaacatttc attgatcaag caaaagaatt
     1081 aaaagggagt gctaaaaatg ccaaaaaaaa aagaaaagaa aattccagtg tttttgtctg
     1141 tctcagaaat gttatcactt caaatactgt taagaaaaaa gattgaaaca atagacgaag
     1201 aagttagaaa taaaagggct atgtacgaac aacgtgaagc gctaagagaa cgaatccact
     1261 attttgatga tatacacact aaaatctatg aagcacgtac aaataacgaa gacgaaagca
     1321 aattaggatt agctcaacaa cgaatatttg aatggctaaa tacgaatact gagcaagaac
     1381 cgttaagagc aattagtaat ctttttgaat ttggaacgaa gcattggttt aacgcagtag
     1441 aaaaggatgc tgacttacaa gaagcctttg acacgctaag taaggatagc aaatccatgc
     1501 gagatttatt gattacttat ctaaatgagc atgagtttga acaaacgaaa accaaaaaga
     1561 aaaataaaga atagaaagtt gtgatgaatc atgctattaa ccctgttagg ttatttatgt
     1621 ttaattcttg ctttttttgg ggcaatcgct ataattattg gtcaatttca aacaaataat
     1681 ccgattatgc gtaacatagg cattggaatt gtgattgtaa cctttattgt gtttaatgcc
     1741 actaagattt ttggaagtaa cgataaacaa tttggtatat cgggagttaa taatagttat
     1801 gctccagatt ctttgggaag ggtagtaata gatggaacat ttgaaaatgt aacgagttat
     1861 aaaaatgttt cgctgacagt gaaggaagat ggcaagaaag catcagacga cgttctcgct
     1921 actattaagc cgtctcaatc acaatttgaa gtgttttata cagttaaaga gaatagccct
     1981 gtacacaagc tgacattggt ttttaacgtt gatggagaag aatactataa agatgtaaaa
     2041 ataaataaaa catcaacgaa aaagattatc gaatcagcga aaaaagaagt agctacagaa
     2101 gaaagtacaa ctgaatcaag ttctactgtc gaagcggaac taccagataa aattgatttt
     2161 gatcctgaaa aagtcaaatc gtttgaatct gtttctggtt ctatggctga tcggtttaac
     2221 actgaagtct tcaaagctat caaaactgtt aaattggata ctgaaaagca aatgattaat
     2281 gttaccttat tcaattcagt tgaatctatg gatcaggata aaaaagttac ggttgcaaga
     2341 gaactaggaa aaagagtaat tggtttgtat cgtggtactg ctaaaccaga ttcagacggt
     2401 gcagatttgc cagttcatgt agtttatgaa gacggtaaag tttttggaga ttctacagca
     2461 aacgaagcat taacgattaa attggctaaa tagccagaaa gttataataa acaaatggtg
     2521 tcacaccaat aatatatagg tgtgacacct tggagatgat agctgatggt agtaaataaa
     2581 gaaaaagtta gaaagtatgt gactttaaaa atagcttctg ttaaatattt agaaaagcga
     2641 gctaaagaag aagaaagagc aagaggtaag aaagtaacag tttcagagtt gttagaagaa
     2701 ttgatcgaga atgatcaaac aataagacgt gcatttacta ataggtctat cagataacaa
     2761 tataattaat aaaaaagaaa gagtgatttg gtcttagact aaatcactct ttttatataa
     2821 agtttcttta cactcattcc gaataatgtt aaaattcttt atatgatata tagttccttg
     2881 tcagaggaag agataaccag caatattgga gggataataa gtggaaaaca ataatgatat
     2941 tagacatttt agcattgggg aaagagttac aacagtaagt gaattatcaa aggattatag
     3001 agataaagag ggaacgatta tttctcttaa taagaatcga agaggcgttt ggcaatatgt
     3061 tgttgattta ggtgatggcg ttacagttga gtgtaaagat agtcaactga tcagaaataa
     3121 tggcagtcat tccattgaaa aagcaattct tgtataccca aaagtatcaa gtaagatcat
     3181 gaatgactgg ttgcttggaa aatttggtga gtacaataaa aaagaaatcg cagaagtgat
     3241 cggcaaatct tatccaacag tattagggta tctaaaaaag ataacgagta aagaagcaaa
     3301 aattgaggat gttttaacag taagagagct tcaatcatta atcgagtatc ggaataaaca
     3361 aataacaaat cctgagtacc ttagaaaaga agatggtcta ttgaaacaat tggaacaagt
     3421 aaataaaatc cggaagatgc aaaaggtagc tcctttaagc cttgatacct ttaaaaaaat
     3481 ctatttgaaa gagatcaaaa aagactaatg tggactgtaa ctttaccgtt ccaacctgta
     3541 tctgcaccaa gaccaagtgc aagaaggaat gctgctgatg aaactaatga cgtagcagag
     3601 aaaaggattt caacctacta tgataaaaag tatcttgatt atttgaaaaa aataaagagc
     3661 tttatcgaag atcaagattt attagatgat gaattttact cgattgtaaa tgatcctatg
     3721 ggcgcaatta tggaagtgga cttttactac cagataccaa aaagctataa aaaagtctat
     3781 aacattatga agacaacagc gccagattta gataatttag taaaaagtgc tatggatggt
     3841 atttttaaca tctcagctat acgagatagc catatcacag gcttaattgc ttttaaatat
     3901 aatgtggcga acgaaggaaa aacagttgtg aggatcaaac gatattcaga atttgagtgg
     3961 gatacttctg aaggattgaa cggagatata tgggaagcta catttgattt taaaccagta
     4021 gcaacgccaa gaccaaagtc taaatttaga ggaaatggga ttatcactta ctatccaaaa
     4081 gagtacaacg actatttgga aaatatcaag aatacgctta aagagaaaga tttggtaaat
     4141 gatcaactga aaaaggtaat gaatgctcct atgggggtta tagctcagat agattatttt
     4201 taccaagtga gaaaagataa gaaaaagctt gattcactga tgcgtacttc acagccggat
     4261 attgataatt tagtcaaagg aacattggac ggaattttta acaaggaaat aggcatcaaa
     4321 gatagcaggg ttgtagggtt aattgctttt aaatacaata cttttgaaaa gtcgaaaaca
     4381 aacgtgagac tacaggagat gggctgatga ataataatga aaaaattctt catgtacttg
     4441 atagctttga aataattcaa gaagaactaa aaaaatatcg ggatgttttg gaacaaagat
     4501 atgattttgt caatcagcaa aagagtaatc acatggattt cattctcaat atgaatgact
     4561 tgaagaagaa attagttgaa cgaaaagaac aagaaaagtt gatcaaagct tactttgaac
     4621 tcggtgaaaa agaagttaaa aatgcaatgg aactgaatga agagcgcagg gttttagatc
     4681 agctgttaga acaattattg gtgatgtttc aaaaaggacg aatagatgag gatttaattg
     4741 aagaaggatt gagaaaatat ccttcaaact caggtatcgg aatagtgctg aaagcgattg
     4801 atgaagaaga aattgaagct ttcataccgc cagaggattt tgaatcggca atggaatata
     4861 taaaatatta tagccaaggg atcactgctt ttagagagtt tgatcctgaa gatgttattc
     4921 atgacttgaa caacctaaaa gaatggtgtg aaagttatgg agtcgatgat tccggattgg
     4981 actacttgat cagtattatg gaaatcgaag aagagatgcc ggataagcct gatccaacag
     5041 atattttaga attgatccat gaagctagaa atccaattgc ctatatttct agaggatata
     5101 ctgtattgga atactataag ccttatatat cagcgatgaa ccatttaaga cgtgtattaa
     5161 gggaaaaacg tgaataccgc tcagtgttga atgcaacgaa tagattagaa aaagctgttt
     5221 cagagctaga tgcttattat cgtgaacatt acctgcaagc aggtggtatg ccaagaaata
     5281 cgaaagcaaa tatcagtagg tatataaaaa aagctgagta ggcggagtta gcccataaaa
     5341 tggcaaaagg taacctgaaa ggggcatttt gaatgaaaag ggatgttttt gaattgtacg
     5401 tattagaacg tctagaagac ggcatgatgg cacaagagat aattgatgaa attgatgata
     5461 tgatcagcag atttggtcaa ttggagttga atgatggaaa caaaaattcg gtagtgctaa
     5521 aacaattttg gttgaacaat ttttcgtata aggattttat caaagaaaag gcaaatacat
     5581 tgtactgact aaattcacga ggagcagtga tgagagctta ttgctaagag cagttaggct
     5641 cttttttgtt tgtgtaaaat tatttgtttt ataatacaca tttatacaca aaaaaatatt
     5701 gacagatagt acacaaatgt ggtaggatat atttgtaagc aagttagatt aacaagttaa
     5761 aggaagagca gttatgaaaa aagaagaact aatagatata tttcaaattg ttgaacgagc
     5821 aaataatatg ggaattatgt tttttgatcg tatttcttta aagatg
//
LOCUS       DASJTM010000136         5774 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJTM010000136 DASJTM010000000
VERSION     DASJTM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.138"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="U4B29_002718"
     CDS             complement(488..1297)
                     /locus_tag="U4B29_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2107612.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="U4B29_002719"
     CDS             complement(1297..2043)
                     /locus_tag="U4B29_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107613.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="U4B29_002720"
     CDS             complement(2061..2528)
                     /locus_tag="U4B29_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107614.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="U4B29_002721"
     CDS             complement(2528..2938)
                     /locus_tag="U4B29_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2107615.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="U4B29_002722"
     CDS             complement(2949..3962)
                     /locus_tag="U4B29_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2107616.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="U4B29_002723"
     CDS             4179..4913
                     /locus_tag="U4B29_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107617.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="U4B29_002724"
     CDS             4897..5607
                     /locus_tag="U4B29_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107618.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
     gene            5661..>5774
                     /locus_tag="U4B29_002725"
     CDS             5661..>5774
                     /locus_tag="U4B29_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2107619.1"
                     /translation="MSHFKGKQFHQDVIIVAVGYYLRYNLSYREVQEILYDR"
BASE COUNT         1854 a         1209 c          961 g         1750 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttcat caggatgtga
     5701 ttattgtagc cgtgggctac tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa
     5761 tcttatatga tcgt
//
LOCUS       DASJTM010000137         5687 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJTM010000137 DASJTM010000000
VERSION     DASJTM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.139"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(169..951)
                     /locus_tag="U4B29_002726"
     CDS             complement(169..951)
                     /locus_tag="U4B29_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107620.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1016..1852)
                     /locus_tag="U4B29_002727"
     CDS             complement(1016..1852)
                     /locus_tag="U4B29_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2107621.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1870..3279)
                     /locus_tag="U4B29_002728"
     CDS             complement(1870..3279)
                     /locus_tag="U4B29_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2107622.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3293..5116)
                     /locus_tag="U4B29_002729"
     CDS             complement(3293..5116)
                     /locus_tag="U4B29_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2107623.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1732 a         1242 c          941 g         1772 t
ORIGIN      
        1 acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt cgggctccaa
       61 atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta taagtacggt
      121 catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt attcaataac
      181 ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca cagaataatc
      241 ttttttccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct ctgtcactgc
      301 attactgata cctgttagat tagctaataa atctgataaa gattgagcat ctgtagaaat
      361 ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc aagtaggtgg
      421 tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct tcccagtatc
      481 tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct gctccgtaac
      541 tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta tatattgtcc
      601 atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac tataaccagt
      661 tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa aatagacatg
      721 taaaagcatt ttgttgttta catctacata ataatccttt gactctactt gagaccttcc
      781 tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat tattaatctg
      841 ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact ttaccacttc
      901 actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca aggaatcagg
      961 agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc ttcattcagt
     1021 acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct gaggcaccca
     1081 tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc ggaaaacgct
     1141 gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc ttaatcattc
     1201 gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa
     1261 cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt gctcttaccg
     1321 agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg tgaaacgcaa
     1381 tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc aattggatag
     1441 catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc agcaggcgat
     1501 tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga tcaaccaagg
     1561 taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa tcgataattc
     1621 gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg taatcctttt
     1681 ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca gccggaacca
     1741 aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact tctttgcctt
     1801 gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc attagattca
     1861 ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa agaagctttc
     1921 tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat atataaaacc
     1981 ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc aaggtaaata
     2041 accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat gtttggctag
     2101 ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt cgggtgcccc
     2161 atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt tcctcaatgt
     2221 taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta cataaggatt
     2281 ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc ccgctctagc
     2341 ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat aataattaat
     2401 cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct caattttagg
     2461 atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc cccgaactgc
     2521 tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc
     2581 tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga tttttccttt
     2641 aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa catggtgatt
     2701 aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac caatcgctcc
     2761 aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca ccttgtcacc
     2821 atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg ctcgatttaa
     2881 ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg
     2941 ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa ttcccttttg
     3001 attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc
     3061 ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt aaaaatccga
     3121 ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa tagacggttg
     3181 tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg caccccataa
     3241 aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct ccttaactaa
     3301 tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat
     3361 tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt ctaatggcag
     3421 cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct tttacaaata
     3481 cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc aataattcaa
     3541 taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta atgacttctc
     3601 cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc tttcccaata
     3661 gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt ccttttaccg
     3721 gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca gaagtatcgt
     3781 caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca cacgtaataa
     3841 ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct gctaagccag
     3901 gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca gcaattgcac
     3961 caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca tagagtgaag
     4021 gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc ttcaactctt
     4081 tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata ttagaagcca
     4141 gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata gtagcgattg
     4201 gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa
     4261 atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct ataagatcgt
     4321 tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata attaaaaata
     4381 ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc gcaaagggct
     4441 ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc gcataatctg
     4501 cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa
     4561 tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg acatcaaact
     4621 ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca tcagcaatag
     4681 tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa gtcaagacgg
     4741 ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg
     4801 tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa gaggggccct
     4861 ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg acttcattgc
     4921 caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca agtgtttttt
     4981 ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt ctggtggcac
     5041 aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca tttcttccta
     5101 cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac
     5161 gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat cagtaacatg
     5221 ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc aatcaacagg
     5281 gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt tagatcacat
     5341 caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat attaactata
     5401 ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa
     5461 atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat tgatgaattt
     5521 ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata gtgatacttg
     5581 tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga tataaaggta
     5641 tcgatagaat gatttgggct agtattctta aaaattaact agcaaat
//
LOCUS       DASJTM010000138         5663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJTM010000138 DASJTM010000000
VERSION     DASJTM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.140"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(210..848)
                     /locus_tag="U4B29_002730"
     CDS             complement(210..848)
                     /locus_tag="U4B29_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2107624.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            1183..1617
                     /locus_tag="U4B29_002731"
     CDS             1183..1617
                     /locus_tag="U4B29_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2107625.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1640..1960
                     /locus_tag="U4B29_002732"
     CDS             1640..1960
                     /locus_tag="U4B29_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107626.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            2101..2292
                     /locus_tag="U4B29_002733"
     CDS             2101..2292
                     /locus_tag="U4B29_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107627.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2363..2620)
                     /locus_tag="U4B29_002734"
     CDS             complement(2363..2620)
                     /locus_tag="U4B29_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN2107628.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2778..3041
                     /locus_tag="U4B29_002735"
     CDS             2778..3041
                     /locus_tag="U4B29_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107629.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            3065..3253
                     /locus_tag="U4B29_002736"
     CDS             3065..3253
                     /locus_tag="U4B29_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107630.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(3336..3521)
                     /locus_tag="U4B29_002737"
     CDS             complement(3336..3521)
                     /locus_tag="U4B29_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107631.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3736..4107
                     /locus_tag="U4B29_002738"
     CDS             3736..4107
                     /locus_tag="U4B29_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2107632.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(4257..4329)
                     /locus_tag="U4B29_002739"
     tRNA            complement(4257..4329)
                     /locus_tag="U4B29_002739"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4294..4296),aa:Gln,seq:ctg)
     gene            complement(4375..4803)
                     /locus_tag="U4B29_002740"
     CDS             complement(4375..4803)
                     /locus_tag="U4B29_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107633.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4863..5621)
                     /locus_tag="U4B29_002741"
     CDS             complement(4863..5621)
                     /locus_tag="U4B29_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2107634.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1929 a          902 c         1125 g         1707 t
ORIGIN      
        1 caaaaaaaat ttgtgtgaag gtgggacagt ttaaaaccta atagattgat tcaaatagta
       61 gatttataga ttctgataca agaaatactg gcaaacgata gttaaggaat aaagactaaa
      121 cttaaactca aaaagaggtc atgcgaaaga ataaatcgca tgacctctta ctatattttg
      181 aatgtgttta aacgttcaaa taatgaatct tagtaccaac cgtttgcttc ccagaatgct
      241 ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg
      301 tttgcagcag agtagtcacc atttaagtaa gaagaatcta attggtaacg gccgatgtaa
      361 cgtccgttcg ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca
      421 gagcttgtac ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca
      481 gcaacaggcg ctgcttgtgt ttccgtttga accgtttctt gtacaacagg ttgttcagct
      541 tgaacaggtt cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct
      601 tgtgcagagc cttctgttgg gattgttaat tgttgtcctg agtagatcag gttaatatca
      661 gaaattgaat ttgattctgc aatggcattg atcaaggagt tatcaccaac gtatttttga
      721 gaaatcgttg atagagtatc acctgattga acggtataag cttcgtccgc atgagcagtc
      781 gttcccatga agaatgcagc gccggcagcc aaagttgttc caaaaagtaa agttttaagt
      841 gatgtcatga aaaatctcct ttattcgtta aaaagtttta tttgttgtgt tcaacgagga
      901 atactttaac acgtgaaaat atttcatagg taaaagaagc atgacaattt atgacaaatc
      961 cacgtttttt gtaacagaat gatgttcgct cgatgtgaaa aatgatgagg aatttcacat
     1021 aatgcagaaa taatgggatt agaacaggtg ttctgatgag aaaagaaatg tataaataac
     1081 ttgaaagaaa ttgcttttgt tcatcatttt atttacatat tgcgtatatt ttttgtattt
     1141 ttgcttgaga tgttacaata aaagaaaaag gaggcggttc caatgagtaa aagataccag
     1201 aacatcatgg tagcagttga cggatcaaga caatcgatca aagcgttcgc tgaagccctt
     1261 gacttagcaa aggacaatga agcccactta tttatcgtgt caatcatcaa caaagttgag
     1321 ctgacccata gtgcctatgc cttttcaaaa atctatgcag atgaaaaaca aagaacagaa
     1381 gtagccatgc tgaaaaaaat caatgatgcc aaagagtacg gcattgctga gatccacgca
     1441 atagttgaaa ctggagatcc aagaaatttg attgccaatg tcatccctgg acaagaaaat
     1501 attgatttga tcgttatggg agcgacagga aaaggtgcta tacaacaagc attagttgga
     1561 tcgaccgctt cttatgtcgt atcacatgcc ccttgcagtg tgttggtcgt gaagtaatga
     1621 gataagggag gaatagaaaa tggttcgtcc tcaagaagtc cgtgcgccaa aagaaaaaat
     1681 agaaatttta gcaattattg aagacggcac acaaacaaag aagggatact cgatcgcttt
     1741 agtaaagtgg aaaggaaaaa aaggtgttgc tctgagatgg gatggagata accaacaaga
     1801 taaaggattc cctgtcacag ctaacggtta tcatccagcg tggtttgttc tgcctgataa
     1861 atttatggaa ttatacagct atgattatgc aagagccgca gcatcaatca aagcattaga
     1921 taaattaaca gaagagcaga atgaaatgga ggaaaaataa aaaagacgaa ccaatcagcc
     1981 tttagaaaac tgattagttc gaggagtaaa aatgaaaaaa ggttatttgg gatgaaacta
     2041 gaataatgcg tataacttaa ataaaactta aagcaataaa atggcaagag ggtgaggcaa
     2101 atggaagcag gaaaagtatt acaggaagta aaagcactat gtgcagataa aaaatatgaa
     2161 gaagcgaaaa gcctgatcga aaagaacaaa gaaaatttgg aagaaaagta cccgatcgca
     2221 caagaattta tagaattaaa acaaggaacg atccttcacc gattcaaaca tttttttggt
     2281 ataacagaat agaaaaaacg attaaagatc ggcagaaatt tgctgcatct ttaatcgttt
     2341 tctttttaaa catgcgaggg attcaattct gattcttata taaaaaatag atccaaataa
     2401 taagaaaaat tactgaaagc gtcaattcga tgatgtgtgt tattaataca gacgttgttt
     2461 cccaatgcct aaaagaaaaa atttgataag taaaatatac agcaggtaaa gaggccagta
     2521 aaactgaaaa cttctttttt gtccatttta tgtaatagaa aagaatgctt atcataataa
     2581 aaaaaggaat ataaaaaaca aattgaccga tcgcgatcat actattgcct ccgataaata
     2641 aagtccatga tacaaaataa cactttgtgc gaaatggcgc aagcagtttt gtggatttat
     2701 ccggtaaaat ctgtatcgat tttgaaatta gaggagaaac ttgctataat caaagtgaaa
     2761 gaaggagtgg gattttcatg gatcatgtag tgttagaaat cgatttgcat ttgatcaata
     2821 atattcgggt gatttactat gttgtaagta acagtgtcga acaacgtgta ctgacgaata
     2881 aaataaatgg tattttagca aagaaagacg ttcaccgatt caataatggt gaaggaagtt
     2941 attatagtat cccagtagaa aaaattattt atacgacagt caaagtcaga gaagatctag
     3001 ataccaaaaa agcatatgaa cctattttta caacttattg attgacagca gatggaggaa
     3061 aaaaatgaat ccatatgaac aacttattca agatttgatc gatggaaaaa tcgaaaagat
     3121 tgatgtaaaa agagaagaat taatggcttt tcgagaagcg tggctgaagt tggagaatcg
     3181 taaatatgtg gtgggaaaag cctcactgaa cggcaatgtg acttatcatt acgatccgac
     3241 acgtttattt tagtatgaag tattcagaaa agaaccaaag gaggacgatg cagcagcatt
     3301 gtcctccttt gattttacaa aagtgaaagt tttacttatt tgaatagatt tttaaatttg
     3361 tttttgacac tgtcgatatt tacatctttt aaaagatttt gggctttctc tttataaggt
     3421 cctaattgtt ctttatgttc ttcgataaat cgttttgcgc catcgaaatt gccgttctta
     3481 atcatttcct tgactttttg aaataattct tcttgattca ttttgaacac atcctttgct
     3541 atcaacatga ttttttacat ggacaaaacg agaaaaactg atttatccac acgtcaataa
     3601 tatcaaataa cgatcatttt tactaaaaaa acactcgatt ctcgcttcta aagtaggaga
     3661 aaaatctcca acttgggacg gatggaattt ctttaactga cccatatact gaatatataa
     3721 caaggaggta gaaagatgaa agtgattaaa tatttgattg gactggcggc gattagtggt
     3781 attttgctat ttggactgaa atattatact gaaggaagtt atggagaaat ccctggtata
     3841 cttgatcagc taaatccatt agttgaaaaa ggagaggtct atgtaaagac acaaaaacca
     3901 gaggaagtaa atgaatatgg tacagcaagg tatgtgcaga aagctgctga tgccaatggg
     3961 aaagaacgaa cggttgaatt taatggtctt tctgtgttaa aagaaaatca ttatttgaaa
     4021 attagcaata aaggagcaca catagaaacg tatgaggaag ttcctagaga aaaagttccg
     4081 gagaaagcat tagcaattat tgggtaaatc agtttttgta ttaagaatac gcccgatttt
     4141 attattcata atttaattat gtaaactaaa tacaagtagt aaaaataata caataatccc
     4201 cctgcaaaaa gattgttctt ttacaggggg ttcatttttt cagcaagcac atcaaattgg
     4261 gatggcaggg ttcgaaccta caattgcttg attcagagtc aagtgcctta ccagtttggc
     4321 tacatcccat ttattcaaca aaataatcat agcactttcc taaaaaaacg caagttattt
     4381 ttcttgatcg gatagttgtt gctttactcg taaaaggcgg cgtactagtt tcttttctaa
     4441 tccagcagga agggcaatca gttctgctac tgacaagacg gtgtccaata actgatattc
     4501 tttgacaggt tcattcataa tatgatagat gtttgctaaa cgttcaatat attcaaagcg
     4561 agtatactct gttcgagcaa ttagaaattt caataaatcg gtggctttct cgtactcttg
     4621 atctgcgtaa taaacaagag attgacgata caatttgatt tccagttcat tgagggtagc
     4681 aggtctgcga cgctcgttcc actcattaat agagacttta ccttgaacat ttcccttgca
     4741 gtcacggaca aaaataatcg gaatttcatt tggatccatt tttttatgtg tgaatttttc
     4801 cattatctac gctcccttca aaggtttggt atcttgagta taagggattt ttgtttgaat
     4861 tactagcttt tctccaaagt tagcatctgt ccacccattg cagtgaaaat cgccgaaaaa
     4921 tcgttgatcg gtactgctcg attttttaca ccatagggat cgttgacata aatattttct
     4981 ttgtcatatc cagtgactac acaagcatga acaagaggag tgactcttac ttctccatta
     5041 tttgtggtcc aagtcataaa atcagtttca tctgggactt caaaattgat agtcacagaa
     5101 gtccagacag gatttccttt agatagtgct tttttgatat catcgagttg agcatcggtt
     5161 tttggaaaag tgtgataatc cgtttggacg atttgctgag caagggaggc aactggggga
     5221 acatagacga ccatagccca gtctccgcca ctgatatcac caacaaatcc ttcattagga
     5281 tcgccatgag tcccatctgc attgaaaaca gggacgtaat ccaattgctc agctaactgg
     5341 tttttagttg tttcataacc ataaaaagat aaaagcatag aaagcgaagt gacttcacag
     5401 ccattttcta aagaaggaga atcaaactgg ctctctaatg gaacatctag taaaacttct
     5461 gctttctctt ttgtcttttt cgttttttgt gttgaacttt tttgtgcagt gttcattgta
     5521 tgttctggag aatggatatg taggtaataa cttgtaccgc ttaataaaag agcagctgtg
     5581 ccccctagaa acaatagttt tttgaacgtt tttttcttca tttgaaatct cccttttcag
     5641 ctgtttatct ttcctattat aaa
//
LOCUS       DASJTM010000139         5645 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJTM010000139 DASJTM010000000
VERSION     DASJTM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.141"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..1271)
                     /locus_tag="U4B29_002742"
     CDS             complement(join(109..995,995..1271))
                     /locus_tag="U4B29_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2107635.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            1341..1502
                     /locus_tag="U4B29_002743"
     CDS             1341..1502
                     /locus_tag="U4B29_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN2107636.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            1521..2798
                     /locus_tag="U4B29_002744"
     CDS             1521..2798
                     /locus_tag="U4B29_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107637.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            2808..3464
                     /locus_tag="U4B29_002745"
     CDS             2808..3464
                     /locus_tag="U4B29_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107638.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            3464..4840
                     /locus_tag="U4B29_002746"
     CDS             3464..4840
                     /locus_tag="U4B29_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107639.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(5126..>5645)
                     /locus_tag="U4B29_002747"
     CDS             complement(5126..>5645)
                     /locus_tag="U4B29_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107640.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         2022 a          799 c         1010 g         1814 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactgag
      781 ttgaagccca cgctttgcta aacacttctt caattttcgg gtaccgtaag cctttcggtt
      841 tcgaataaac tcttcctgaa cgatctcttc taagtcggac tcattttcga tcggtttcgc
      901 ttgataatag taggtctgac gagaaatatt tagaattttg cacatcgctg atatggaata
      961 tttatgcttg ttggcatcaa ttacttgtct tttcgtccga atatcagcgc cgcttgcttt
     1021 aaaatatcat tctccatttc gagttgctta tttttctttc ttagtgcgat caattctgct
     1081 tgttctggtg ttaagttgtc tctttctttg aatgagcccg ttgattgtgc ttgcttcatc
     1141 catttatcga aagaagaagg cgtaagctca tactctcgaa taatttctgc gcgaggctta
     1201 ccagcgagat agagatcgac gatttgttgt ttgaattctt ttgaataggt tcttcgttga
     1261 cgtcttggca tgaaaaatcc tccagtgtgt tttttattat tctacacacc ttattttttc
     1321 tgtctagtct agtgtagccg attcaggata aggataagtg tattaattgt ggaaaatgta
     1381 aaaaagtttg ccaaatgaat aaagattcag ttgagaattg caatcatctg gagtgtatca
     1441 gatgtggacg atgtaaaaat gcttgtccag tggatgcaat tagttatgga gtaagaaaat
     1501 agagaggagt agttaggaaa gtgataaaaa atgctatagc atatattaca agaaagaaaa
     1561 atagaacttt aataattttt atcatattaa ctatagttct ttcttgcttg tattcttgtt
     1621 taacaataat gaaatcaagc aatgaaatag aaaaggcttt atatgaaagt tctaattctt
     1681 caatatcaat aacaaaaaaa gatggtaaat attttaatgt taatcaattt aaagatattg
     1741 aaaaattaaa agaaattgaa gaaaaaataa ttcaatatga tggattagca aaactaaaag
     1801 atgctaaagt agttagcgga gaacaaagaa taaatagaga agatttatct gacgaattta
     1861 agaatgttgt ttcactcgaa gctacaaata atactaaaag aaatatttta tttagtagtg
     1921 gagtatttac aattaaagaa ggtaaaaata tagaagaaaa tgataagaat tcaattattg
     1981 ttcatgaaga atttgctaaa caaaacaatc taaaattagg tgatgaagtt gatcttgaat
     2041 tactagatat tgaaaaaagt ggaaaaataa aaagtcataa atttaaaatt atagggatct
     2101 tttctggtaa aaaacaggaa acatatacag gattatcttc tgattttagt gaaaatatgg
     2161 tgtttgtaga ttattcaaca agccaagaga tattaaataa ttcagaaaat aatgaaattg
     2221 caaataaaat tttaatgtat tctggtagtg cagaatctac agacttagcc ttaaacaaat
     2281 tgaaagagct taaaattgat gagtcaaagt attttgttga aaaagattct aatgcgtttg
     2341 aagagtcttt agagtcagtg agtggaataa aatatataat aaaagtaatg acttattcta
     2401 ttatgttagg tgggatggtt gttctttcat taatcttgat tctatggtta agggaaagaa
     2461 tttatgaaat aggtatattc ttatctattg gaacatctaa gatacaaatt ataatgcaat
     2521 ttatattcga gttaatattc atatcgatac caagtataat atcttcctta tttttaggaa
     2581 atgtattact aaaagtaatt gtagatggat tcattaactc agaggattca atgatttccg
     2641 gtggaagttt aataaataat agtagtttta tgtcaaatat aacaacactt ggacaaagtt
     2701 atttaatatt aataagtatt attgttttat cagttgtatt tgcttcttca ttaatgttaa
     2761 tcaagaagcc taaagaaatc ttatcaaaaa taagttagga gaaaataatg gatatattag
     2821 aaataaagaa tgtagcatat agttatgcaa attctaaaga aaaagttttg tcaggagtaa
     2881 atcaaaaatt tgaacttggg aagttttatg cgatagtagg aaagtcagga acaggaaaat
     2941 ctacacttct ttccttactt gctggacttg ataaacctca aacaggaaaa atattgttta
     3001 agaatgaaga tatacaaaat aaaggatata gtaatcatag aaaaaataat atatctttag
     3061 tatttcaaaa ctataattta atagattatt tatcaccaat tgaaaatatt agattagtaa
     3121 ataaatcagc agatgaaagt atcttgtttg aactaggttt agataaaaaa caaataaaaa
     3181 gaaatgttat gaaattatct ggtggacagc aacaaagagt agctattgct agagcattgg
     3241 tatcagatgc tccaataata ctagctgatg agcctactgg taatctagac agtgttactg
     3301 ctggagaaat aattaatata ttaaagacat tagctaaaga tagaaataaa tgtgtaatag
     3361 ttgtaacaca tagtaaggaa gtagcagatt ctgcggatat cattttagaa ctaagtggta
     3421 aaaagttgaa aaaagtaaat aaaatgaatt tggaggttga ataatgataa aaaatgcatt
     3481 tgcttatgta actagaaaaa gtttaaaatc attaattatc atattagtta ttttatctat
     3541 gtcaacttta agtctcatta gtttatctat taaagatgct acggacagag cttcaaaaga
     3601 aacatttgct aatataacaa atagtttttc tatggagata aatagacaag taaatccagg
     3661 aacacctaga ggtgggggaa atgtaaaagg tgaagatatt aaaaagatat ctcaaacaga
     3721 tagtatagac tcctatgtaa aaagaataaa cagtgttgca gatttagttg attatgatat
     3781 tattgaaact caagagactc ttgcgaatca atcacctgaa agagcaaaga attttaaaag
     3841 aacagttatg ttaactgggg ttaacgactc aacaaaagaa acaaaatttg tatcagaagc
     3901 atataaatta gtagaaggaa agcatttaga aaatgaagat aaaaataaaa tcttaatgca
     3961 taaagattta gctgaaaaaa ataaccttaa agttggagat aagataaaaa taaaatctaa
     4021 tttatttgat gctgataatg agaaaggtgc agatgaaaca gtagaagtag aaattaaagg
     4081 tctattcgat gggcataata gcggtgtagt aagtgcagca caagaactat acgaaaatac
     4141 attaatcact gatattcata cagctgctaa agtttatggt aataccgaag atacagctgt
     4201 ataccaagat gcaacattct ttgtaaaagg tgataagaat cttgatagtg taataaaaga
     4261 tcttgggaaa ttagatataa attggagaga atataatttg attaaaagtt catcaaatta
     4321 ccctgcatta caacaatcta tatcaggtat ttactcaatt tcaaataaat tattttttgg
     4381 ctcattaata tttgcaggag ttgtagtttc attattatta ttcttatgga tgaatgctag
     4441 aaagaaagaa atagcagtat tactatcatt aggtatatca aaattagaaa tttttggtca
     4501 attcctaatt gagatggtat ttatatcaat ccccgcattc gttggttcct atttcttagc
     4561 tcaatataca gctgacaagc ttgggaataa tatattgaat aaggttactg gtgatatagc
     4621 taaacaaata gctaaacaat ctgcatctag ccaattaggt ggtggggcag aagctgaagg
     4681 atttaacaag acattatcaa gtttagatat caatgtatta cctaaattca tgatttatgt
     4741 agttatattt atgagtttag tacttcttgt atcattgatt atttcttcat tcaatatatt
     4801 aagaaggaat ccaaaagaat tattaattga caataattaa aattttcggt gctttttagc
     4861 accaaaaatg agagtgtaat ataaaccgtg taagtaacca tcaatcctag gcgggtggtt
     4921 gcttcacggt ttttattttt ctaaaataag atcaagaggt gattacacga tggctagaaa
     4981 gaaaagaaat cctgatgctg agaagttagc tgaatccatt ttgaatactt atcaacccga
     5041 atctgtagag gatatgcaag atgttttaaa agatgtgttt ggtcccttat ttgaaaaaat
     5101 gcttcaagga gaattgaata accatttagg gtatgacgcc cattctaaag agccaaagaa
     5161 acatgacaat cgacgaaacg gttatggcaa taagacgctt aaaacgagct ttggtaaagt
     5221 aaacattgat gttcccagag accgggaggc ttcttttgaa cctgagttga ttcctaatag
     5281 aaaacgagat gtctccgaca tcgcagactg tagacaaaag ctgttccaaa ctcgtaactc
     5341 gagcttggaa cagcttttta tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct
     5401 ccttgccttt ccatagccag gtggctaatt tctttaaatt cattgcagca aacactagca
     5461 tcgtatgcgt cgcgactttt tccaagcctc gatacttggt ccaacgcatc ccatgctttt
     5521 cttttgcatc cgcaaatatt cgttcaatcg tttgtttccg ttttttgtaa attgatttat
     5581 tcagatctgt taggcgaaga tgttccactt catccatcag atctccccaa acatgtctcg
     5641 tgatg
//
LOCUS       DASJTM010000140         5332 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJTM010000140 DASJTM010000000
VERSION     DASJTM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.143"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..592)
                     /locus_tag="U4B29_002748"
     CDS             complement(134..592)
                     /locus_tag="U4B29_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2107641.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(605..1936)
                     /locus_tag="U4B29_002749"
     CDS             complement(605..1936)
                     /locus_tag="U4B29_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN2107642.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1947..3389)
                     /locus_tag="U4B29_002750"
     CDS             complement(1947..3389)
                     /locus_tag="U4B29_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2107643.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3376..4140)
                     /locus_tag="U4B29_002751"
     CDS             complement(3376..4140)
                     /locus_tag="U4B29_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN2107644.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4244..5119)
                     /locus_tag="U4B29_002752"
     CDS             complement(4244..5119)
                     /locus_tag="U4B29_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2107645.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1643 a         1113 c          771 g         1805 t
ORIGIN      
        1 taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg
       61 aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt
      121 ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt
      181 ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg
      241 cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt
      301 tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat
      361 ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata
      421 tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt
      481 atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa
      541 tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc
      601 acctctactt taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt
      661 tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt
      721 gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca
      781 tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg
      841 caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt
      901 gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat
      961 cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc
     1021 ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg accatattag
     1081 cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta
     1141 agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg
     1201 ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta
     1261 gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat
     1321 accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt
     1381 aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta
     1441 cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca
     1501 aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga
     1561 ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat
     1621 gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg
     1681 agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa
     1741 tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata tccatatttc
     1801 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
     1861 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
     1921 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
     1981 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
     2041 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
     2101 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
     2161 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
     2221 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
     2281 ctcttattga tcaaataata aaaagtaacg taatacaaag caaaaaagaa aattccgatt
     2341 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
     2401 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
     2461 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
     2521 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
     2581 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
     2641 aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata
     2701 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
     2761 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
     2821 aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca
     2881 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga
     2941 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
     3001 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
     3061 aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc
     3121 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
     3181 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
     3241 actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc
     3301 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
     3361 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
     3421 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac
     3481 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
     3541 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
     3601 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
     3661 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
     3721 tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc
     3781 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
     3841 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
     3901 tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa
     3961 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc
     4021 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
     4081 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
     4141 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
     4201 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta
     4261 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
     4321 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
     4381 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
     4441 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
     4501 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
     4561 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
     4621 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat
     4681 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
     4741 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
     4801 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
     4861 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
     4921 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
     4981 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
     5041 gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac
     5101 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
     5161 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
     5221 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
     5281 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aa
//
LOCUS       DASJTM010000141         5318 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJTM010000141 DASJTM010000000
VERSION     DASJTM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.144"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U4B29_002753"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U4B29_002753"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="U4B29_002754"
     CDS             complement(580..1353)
                     /locus_tag="U4B29_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2107646.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="U4B29_002755"
     CDS             1497..1847
                     /locus_tag="U4B29_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN2107647.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="U4B29_002756"
     CDS             1828..2544
                     /locus_tag="U4B29_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2107648.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="U4B29_002757"
     CDS             2544..3992
                     /locus_tag="U4B29_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2107649.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="U4B29_002758"
     CDS             complement(4062..4571)
                     /locus_tag="U4B29_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107650.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="U4B29_002759"
     CDS             complement(4561..5286)
                     /locus_tag="U4B29_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107651.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1800 a          933 c         1065 g         1520 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatg
//
LOCUS       DASJTM010000142         5260 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJTM010000142 DASJTM010000000
VERSION     DASJTM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.145"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(642..932)
                     /locus_tag="U4B29_002760"
     CDS             complement(642..932)
                     /locus_tag="U4B29_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107652.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1334..1570
                     /locus_tag="U4B29_002761"
     CDS             1334..1570
                     /locus_tag="U4B29_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107653.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1577..3529
                     /locus_tag="U4B29_002762"
     CDS             1577..3529
                     /locus_tag="U4B29_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2107654.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3601..4098)
                     /gene="dfrG"
                     /locus_tag="U4B29_002763"
     CDS             complement(3601..4098)
                     /gene="dfrG"
                     /locus_tag="U4B29_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2107655.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4425..5117)
                     /locus_tag="U4B29_002764"
     CDS             complement(4425..5117)
                     /locus_tag="U4B29_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2107656.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1701 a          914 c          864 g         1781 t
ORIGIN      
        1 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
       61 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      121 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      181 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      241 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      301 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
      361 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
      421 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
      481 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
      541 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
      601 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
      661 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
      721 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
      781 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
      841 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
      901 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
      961 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1021 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1081 ggcagcagta tagatttaag tagttcagtt ttggagtaca aaaagtgaaa atgtaaacct
     1141 aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa atcaacgttt
     1201 gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg aacgtgaaat
     1261 aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta agcaaaaagg
     1321 ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt ctgtggattg
     1381 ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg cagagcgtac
     1441 tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga agtttattga
     1501 atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta tcaaagatag
     1561 agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag atgaaattgt
     1621 agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac aatatgattt
     1681 attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc caacgtcttt
     1741 taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc cgttttgttg
     1801 taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa gtcgatataa
     1861 aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg acaatcctat
     1921 tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt ctgttgttga
     1981 agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag attatcaatt
     2041 tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga agcaatttta
     2101 caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt gtaagaagtt
     2161 cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa agaacaccat
     2221 tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta cttgtgatat
     2281 tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata ggcgttgttt
     2341 ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta acgagatgtg
     2401 gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag gacaaggttc
     2461 aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg tatctgctga
     2521 ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata tttcaatgga
     2581 tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt ttagtcgtaa
     2641 aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg atagcgaaaa
     2701 taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat ttgtcgatgg
     2761 tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta aacaaatggt
     2821 taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa acgcctttta
     2881 tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt gccaagttaa
     2941 caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag cagaactgct
     3001 cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg catctcatta
     3061 tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta aagatggaga
     3121 aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta aggataaagg
     3181 attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg aaatcgcaaa
     3241 gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga taagacgtta
     3301 tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga gctatattta
     3361 cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa cttactacaa
     3421 cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc aaagattagg
     3481 gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac aaaaagagct
     3541 tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt aacgcacccg
     3601 ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg gatttttatc
     3661 attctttatc cctttttggg caaatacctc attccattcc tcataattca cttctggaaa
     3721 aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct caacataagg
     3781 gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt cgtttttaca
     3841 taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg taaacccctt
     3901 atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg attcaaggtt
     3961 cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt cccagtcctt
     4021 gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat ccatcgcagc
     4081 aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat ctaatttctt
     4141 atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag caattttttc
     4201 tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt atcattttca
     4261 attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat gaaaaaagta
     4321 gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact tagtatagat
     4381 tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt acgcaaatgt
     4441 tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt tgctttttct
     4501 aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc taatttcttt
     4561 tgtgtatctg ttaactgagt ttctacattt gttttttctt gtgtcagtgt tgcgatttgt
     4621 tgattcaaac catttacagt attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt
     4681 cttgctgtcc aatctgcatt cagatgattg atttcatcat ttttttggtt gatctcatct
     4741 agtttgacat tgatttcatt ttgtttgctt tgtagttgtg catccttgct gactaattga
     4801 ctttctaagt tttgtttttc tgctgccgat tgattttttt ggttttctaa ttcatttact
     4861 tttgtttggt attcttggac atctgccttt gaatcttgca atgtctgttc tttttgtgct
     4921 agtcgagcgg taagatctgt aatcgcttgc tctttatctg tcaatgtatg atcaagctgt
     4981 gtcagattct cattgattgt atcaatatct gtctctccac cccacaaaat aatttcattt
     5041 gcgtaccgcg tgcctgtgta gccaattaga catagaaatg caacgacaat cccaccagta
     5101 ataaatttct ttttcatttg catagctcct taatccaata cttttacatg ctaaaagtaa
     5161 gtgatctcaa acacaatgtc aagcgttttt aaattacata tgtaatttta atataaatac
     5221 tatcaaatac tcatttaatc ctcctttttt atctatttaa
//
LOCUS       DASJTM010000143         5227 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJTM010000143 DASJTM010000000
VERSION     DASJTM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.146"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..862
                     /locus_tag="U4B29_002765"
     CDS             5..862
                     /locus_tag="U4B29_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2107657.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="U4B29_002766"
     CDS             884..1393
                     /locus_tag="U4B29_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107658.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="U4B29_002767"
     CDS             1430..1831
                     /locus_tag="U4B29_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107659.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="U4B29_002768"
     CDS             1943..2296
                     /locus_tag="U4B29_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107660.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..2773
                     /locus_tag="U4B29_002769"
     CDS             2330..2773
                     /locus_tag="U4B29_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2107661.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2790..3875
                     /gene="uxuA"
                     /locus_tag="U4B29_002770"
     CDS             2790..3875
                     /gene="uxuA"
                     /locus_tag="U4B29_002770"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2107662.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3868..4581
                     /locus_tag="U4B29_002771"
     CDS             3868..4581
                     /locus_tag="U4B29_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2107663.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4788..4937)
                     /locus_tag="U4B29_002772"
     CDS             complement(4788..4937)
                     /locus_tag="U4B29_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107664.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         1813 a          799 c         1145 g         1470 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     2641 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     2701 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     2761 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     2821 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     2881 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     2941 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     3001 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     3061 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     3121 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     3181 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     3241 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     3301 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     3361 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     3421 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     3481 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     3541 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     3601 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     3661 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     3721 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     3781 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     3841 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     3901 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     3961 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     4021 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     4081 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     4141 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     4201 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     4261 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     4321 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     4381 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     4441 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     4501 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     4561 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     4621 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
     4681 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
     4741 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
     4801 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
     4861 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
     4921 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
     4981 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
     5041 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
     5101 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
     5161 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
     5221 ttttgaa
//
LOCUS       DASJTM010000144         5189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJTM010000144 DASJTM010000000
VERSION     DASJTM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.147"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="U4B29_002773"
     CDS             137..748
                     /locus_tag="U4B29_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2107665.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="U4B29_002774"
     CDS             821..1750
                     /locus_tag="U4B29_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2107666.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="U4B29_002775"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="U4B29_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="U4B29_002776"
     CDS             complement(2693..3871)
                     /locus_tag="U4B29_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2107667.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="U4B29_002777"
     CDS             3969..4067
                     /locus_tag="U4B29_002777"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107668.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="U4B29_002778"
     CDS             4098..4499
                     /locus_tag="U4B29_002778"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107669.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="U4B29_002779"
     CDS             complement(4706..>5189)
                     /locus_tag="U4B29_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2107670.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DASJTM010000145         4947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJTM010000145 DASJTM010000000
VERSION     DASJTM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.148"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1771
                     /locus_tag="U4B29_002780"
     CDS             128..1771
                     /locus_tag="U4B29_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2107671.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(1844..2389)
                     /locus_tag="U4B29_002781"
     CDS             complement(1844..2389)
                     /locus_tag="U4B29_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN2107672.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2412..2897)
                     /locus_tag="U4B29_002782"
     CDS             complement(2412..2897)
                     /locus_tag="U4B29_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2107673.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(2887..3699)
                     /locus_tag="U4B29_002783"
     CDS             complement(2887..3699)
                     /locus_tag="U4B29_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN2107674.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(3838..>4947)
                     /locus_tag="U4B29_002784"
     CDS             complement(3838..>4947)
                     /locus_tag="U4B29_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2107675.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         1593 a          977 c          946 g         1431 t
ORIGIN      
        1 aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg
       61 gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg
      121 aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc
      181 tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa
      241 acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc
      301 tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg
      361 aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat
      421 tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa
      481 atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga
      541 accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg
      601 acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa
      661 attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga tagatggatt
      721 tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa
      781 ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca
      841 tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga
      901 aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat
      961 ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt
     1021 aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca
     1081 tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc
     1141 tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg
     1201 acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga
     1261 ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga
     1321 tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca
     1381 atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat
     1441 caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat
     1501 ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga
     1561 acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt
     1621 aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac
     1681 attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta
     1741 tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga
     1801 ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag
     1861 gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt
     1921 tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac
     1981 cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt
     2041 caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt
     2101 cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac
     2161 aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga
     2221 tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg
     2281 tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat
     2341 taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc
     2401 ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga
     2461 aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca
     2521 gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt
     2581 cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca
     2641 tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc
     2701 aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga
     2761 aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca
     2821 gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt
     2881 aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc
     2941 ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc
     3001 tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa
     3061 ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata
     3121 atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt
     3181 gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt
     3241 taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat
     3301 catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac
     3361 agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt
     3421 ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat
     3481 accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga
     3541 tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct
     3601 tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc
     3661 aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa
     3721 gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca
     3781 atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta
     3841 ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt
     3901 tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac
     3961 ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg
     4021 tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa
     4081 taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc
     4141 tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc
     4201 gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt
     4261 agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt
     4321 aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt
     4381 caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac
     4441 ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt
     4501 gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc
     4561 cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg
     4621 gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg
     4681 aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat
     4741 gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt
     4801 ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga
     4861 agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc
     4921 aggttgttcc attaaataaa gaatagg
//
LOCUS       DASJTM010000146         4939 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJTM010000146 DASJTM010000000
VERSION     DASJTM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.149"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..832)
                     /locus_tag="U4B29_002785"
     CDS             complement(<1..832)
                     /locus_tag="U4B29_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107676.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="U4B29_002786"
     CDS             complement(846..1643)
                     /locus_tag="U4B29_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2107677.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="U4B29_002787"
     CDS             complement(1676..1960)
                     /locus_tag="U4B29_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN2107678.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTLSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="U4B29_002788"
     CDS             complement(1957..2958)
                     /locus_tag="U4B29_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN2107679.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELFKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U4B29_002789"
     CDS             complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U4B29_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN2107680.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="U4B29_002790"
     CDS             4026..4874
                     /locus_tag="U4B29_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2107681.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         1493 a         1079 c          798 g         1569 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgcca catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat aacgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taaataactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtg
//
LOCUS       DASJTM010000147         4892 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJTM010000147 DASJTM010000000
VERSION     DASJTM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.150"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="U4B29_002791"
     CDS             complement(287..649)
                     /locus_tag="U4B29_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107682.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="U4B29_002792"
     CDS             complement(723..1103)
                     /locus_tag="U4B29_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN2107683.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="U4B29_002793"
     CDS             1167..2360
                     /locus_tag="U4B29_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2107684.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="U4B29_002794"
     CDS             complement(2437..3021)
                     /locus_tag="U4B29_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2107685.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="U4B29_002795"
     CDS             3222..4220
                     /locus_tag="U4B29_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2107686.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="U4B29_002796"
     CDS             4332..4841
                     /locus_tag="U4B29_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN2107687.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DASJTM010000148         4767 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJTM010000148 DASJTM010000000
VERSION     DASJTM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.151"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(203..1141)
                     /locus_tag="U4B29_002797"
                     /pseudo
     CDS             complement(203..1141)
                     /locus_tag="U4B29_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(1141..1758)
                     /locus_tag="U4B29_002798"
     CDS             complement(1141..1758)
                     /locus_tag="U4B29_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107688.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(1772..1942)
                     /locus_tag="U4B29_002799"
     CDS             complement(1772..1942)
                     /locus_tag="U4B29_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107689.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(2290..3780)
                     /locus_tag="U4B29_002800"
     CDS             complement(2290..3780)
                     /locus_tag="U4B29_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2107690.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(3823..4122)
                     /locus_tag="U4B29_002801"
     CDS             complement(3823..4122)
                     /locus_tag="U4B29_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107691.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(4488..>4767)
                     /locus_tag="U4B29_002802"
     CDS             complement(4488..>4767)
                     /locus_tag="U4B29_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107692.1"
                     /translation="LAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTIKT
                     LEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT         1365 a          897 c          717 g         1788 t
ORIGIN      
        1 taaaatgatg tataagtgcg cccttttaaa ttttgagtga ttatattttt tgagttagaa
       61 aaagggattg ggaaaatttc ccaaaataat ttaaaaaata agcaaaaatt ttcgatagag
      121 aatgtgctat tttttgtcaa aggtgtatac cttgactgtg cttgctgtta cattaagttt
      181 atttttaagt tattaaaaaa gaaatagctt ttaaagtttg gctcgctgtc gctttataaa
      241 gctgattgac ttttgattgc aaactactta aagaaaacaa actcggacta ttcgttttct
      301 tctctttggt ttgaacatca gcaattatcc cctcttgatt gcctatttta gcttgtttag
      361 aagaaacaaa agctaaaagc tcctcttggg ttttaaaacg ctgtgtgggg cttagaacgc
      421 ccttaaacga cccttggttt acttttatac tagcttccac ctcgaaaaaa ggttcttttt
      481 taaaattctc tatggcttcc tggcgctgaa aaataaggta taaggtgggc gtttgaacac
      541 gtcctagtga aaatgtacct tgtacgccct tctgttgtaa atttaacgta tacaaagggc
      601 ttgcgttcat gccgatcaac caatcggcaa tttggcgtgt ttgcgcttct tgataaaagg
      661 gatagtaatt cattccaggt tgcaaatttt gaaaaccgct tcggattaca tctttttcta
      721 agctattgat ccatagtctt ttaaatgttt tatcttttga aaaggcattt gctttatgga
      781 taatcgacca ggcgatattt tcaccttctc tgtcgctatc tgttgcaaca ataattgtat
      841 ttgccttttt gagaagttct gcaacaattt taaactgctt tcccttatct tttgcaactt
      901 caaaatcgta tcgatcagga aaaatcggca aagattcaag tttccaattt tgccactttt
      961 cgtcataatg acctggttct gctaattcca ctaaatgccc aaaaccaaag gtgataaacg
     1021 tttcatctgt aaatagtggg tctttgatct caaaataacc gtcttttttg gtgctttgtt
     1081 ttaaagcact tgcgtaggct aatgcctggc ttggtttttc agctaaaata accgtactca
     1141 ttaactatcc ctcttttcat tgttttttct ttgatcgact gtcacgttat atcttgctcg
     1201 ataccttcta aacgttcggc gattgattcc agtttgttct tcaacttctt tatcggataa
     1261 accattcaaa aacaaatcga aagcatgctt taaacgtgga tcattttctt taaatttatg
     1321 ctgacgacct ttgaatttgc cttttttctt agcaatttcg attccttgtg cctgacgttc
     1381 cttaattttt tttcgttctg attctgcttg atacttgtac aattcaatga caaggctatt
     1441 aatcaaacgc cttaaatttt catcttcaat accattcatt gagggtaaat ttaagacttc
     1501 cagggttgcc cccttaattt gaatttgatt catcaattct gttaattctt tattatttcg
     1561 tcctaatcga tctaattcag taacaataac aatatcccct tcacgaatat agttaagcat
     1621 agcttgtaat tgtgggcgtt cgaccgattg accgcttaat ttgtctgaaa agaccttaga
     1681 aacgccctgt aacgcttgta attgccgatc taagttctgt tctttgctac tgacacgtgc
     1741 ataaccaatt ttagccattt tcaaccaacc tctaaaattc tctcggttgc aataaccaat
     1801 cagcaatatc tactttttca atttcaaatt gcttatcaga aattgtcttt tcgtaagcga
     1861 taaaatcttg cgcatattgt tgctcattaa aaatagccac cacttcgtca ttttctaaaa
     1921 ctcgataaat aaattttttc attttactcc tcctattatg cccaacttaa atgacctatt
     1981 caccaagtca attatactgc taaaatcata ttaggacaaa taggtatact ctaatgacct
     2041 ataaaagata gaaaattaaa aaatcaagtg ttcgcttcgc tctcactgcc cctcgacgtt
     2101 ttagtagcct ttccctcact tcgttcagtc caagccaact aaaagttttc gggctactct
     2161 ctccttctcc ccctaataat taattaaaat cttactctgt atatttctgc taatcattcg
     2221 ctaaacagca aagaaaaaac aaacacgtat catagatata aatgtaatgg catagtgcgg
     2281 gttttatttt cagcctgtat catagctaaa caaatcgagt tgtgtgtccg ttttagggcg
     2341 ttctgctagc ttgtttaaag tctcttgaat gaatgtatgc tctaagtcaa aagaatttgt
     2401 cagcgccttt atatagcttt ctttttcttc tttttttact ttaatgatcg atagcaacaa
     2461 tgatttaaca ctagcaagtt gaatgccacc atttcttcct ggtttaatct taaagaaaat
     2521 ttcctgattc gccttcagta ccttcagcaa tttatctaat gtccgttcag gaatgcctag
     2581 cacttctcta atctcttttt tggtcgtcac taaataaggc ttgtatacat cgcttttttc
     2641 gctaatataa gccattaaat cttctttcca ttctgacaaa tgaacacgtt gacgttcgct
     2701 tctttttttc ttgaatttaa accacccttg acggacaaat aaatctttac tggttaaatc
     2761 acttgatacc caagctttgc aaagaatggt aatgtattcc ctattagccc cttgatagtt
     2821 ttctgaatag gcacttctaa caattttgat tacttctttt tcttctaagg gttgatctaa
     2881 tcgattatta aactcaaaca tattatattc gcacgtttcg attgaatagc ctgaactaaa
     2941 gtaggctaaa gagagggtaa acatgacgtt attacgccct attaaaccct tttctcctga
     3001 aaatttcgtt tcgtgcaata agagattaaa ccagggttca tctacttgtt ttttgccttc
     3061 tgtaccgctt aaaaccgtta gacttgaacg agtaaagccc ttattatctg tttgtttgaa
     3121 agaccaatct tgccattctt tgaaagaata acggtaatta ggatcaaaaa attctacatt
     3181 gtccgttctt ggtatgcgag caataccaaa atgattacac gttagatcaa ctggcaaaga
     3241 ctttccaaaa tattctcgga tattttgcga aattattttg gctgctttga cagatttaaa
     3301 ttctgatttt gaagtcacat agactggcgt ttctaaaaca aaatatgctt gataaccttt
     3361 atcagatttg ataatcatag taggcataaa acctaaatca atagcggttg ttaaaatatc
     3421 gcttgctgaa atagtttctt ttgccgtgtg aatatcaaaa tcaataaaga aggtattgat
     3481 ttgtcttaaa ttgttttcag aatgtccttt cgtgtatgaa cggttttcgt ctgcatacgt
     3541 tccataacga taaacgttgg gtgtccaatg tgtaaatgta tcttgatttt cttgaatcgc
     3601 ttcctcggaa gtcagaacaa caccacgacc gccaatcatg cttgattttg agcgatacgc
     3661 aaaaatagcc cctttgcttt tacctggctt ggtagtgatt gagcgaattt tactattttt
     3721 aaatttgtac tttaacaagc cgtcatgaag cacagtttct acaacaaaag ggatattcat
     3781 tcagctgttc tcctttccta taaatcctat aaaataggtt gtttaattaa cttggtttgc
     3841 tttttcattc aactgtttca atattgcatg ttttgaaaaa gatttttttc ctttataagt
     3901 caattttttt ccactaatcg aataaattat tttgttattt tctattaact tatatatata
     3961 atcttccccc tccgaagaaa aatacttatc tgattttgtt tctaagtaga tatttctctt
     4021 ttctaactct ttcttaaacg tttctagtgt atagatattt gctaattttc ttatctccaa
     4081 taaactattt tttatataag ttttacattc atcatgattc atacaaactc caccttctat
     4141 aaatgaatac aaaaaaagca atcaaacgat ttccgattga ttgcttaaca attcttaaat
     4201 tcagtagctt agatacttga aaactctctg atttccctat ataatgatag tacggttata
     4261 taccgtcttc aaacaaagtt aattaaataa cttcttacga gggaagagtt catctgacta
     4321 actgataagc gttggtttgg caatcttatc gggctatgca tttataaaat gtcgtcaaac
     4381 attttataaa tgtgtcatgg ctcttttttc gtttctattc agttcgttgt ttcgttatat
     4441 ctagtatacc gcttttaaaa aaaataagca acgatttcgt gcattattca cacgaagtca
     4501 ttgctttttt cttcttccat ttctaaatcc aatgttactt gttctgattc tgtttctggt
     4561 tctggttctg ttggctcatt tgggattaaa tccactacta gcgttgagtt agttaacttt
     4621 gcaatttgtt ctagtgtttt tatggttgga tctgattttc ctgattctat tcgtgaataa
     4681 tttgatctac tcatttctaa ttcttgggag aatttttctt ttgatttggt acctctcatc
     4741 ttttttaagc tttctctaaa tgctagt
//
LOCUS       DASJTM010000149         4750 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJTM010000149 DASJTM010000000
VERSION     DASJTM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.152"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B29_002803"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B29_002803"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN2107693.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U4B29_002804"
     CDS             complement(1297..2133)
                     /locus_tag="U4B29_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107694.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U4B29_002805"
     CDS             complement(2317..3534)
                     /locus_tag="U4B29_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2107695.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U4B29_002806"
     CDS             complement(3683..3862)
                     /locus_tag="U4B29_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107696.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U4B29_002807"
     tRNA            3917..4000
                     /locus_tag="U4B29_002807"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4750)
                     /locus_tag="U4B29_002808"
     CDS             complement(4240..>4750)
                     /locus_tag="U4B29_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107697.1"
                     /translation="YVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLN
                     NLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCS
                     RLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGIC
                     TFIITVINR"
BASE COUNT         1435 a          998 c          766 g         1551 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa
//
LOCUS       DASJTM010000150         4722 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJTM010000150 DASJTM010000000
VERSION     DASJTM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.153"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="U4B29_002809"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="U4B29_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN2107698.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="U4B29_002810"
     CDS             724..1878
                     /locus_tag="U4B29_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2107699.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="U4B29_002811"
     CDS             2093..3061
                     /locus_tag="U4B29_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2107700.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="U4B29_002812"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="U4B29_002812"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN2107701.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="U4B29_002813"
     CDS             4091..4699
                     /locus_tag="U4B29_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN2107702.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1456 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aa
//
LOCUS       DASJTM010000151         4695 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJTM010000151 DASJTM010000000
VERSION     DASJTM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.154"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="U4B29_002814"
     CDS             complement(136..1098)
                     /locus_tag="U4B29_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2107703.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="U4B29_002815"
     CDS             complement(1100..2539)
                     /locus_tag="U4B29_002815"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN2107704.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="U4B29_002816"
     CDS             2724..4670
                     /locus_tag="U4B29_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN2107705.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1486 a          807 c          776 g         1626 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atgga
//
LOCUS       DASJTM010000152         4548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJTM010000152 DASJTM010000000
VERSION     DASJTM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.155"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..790
                     /locus_tag="U4B29_002817"
     CDS             <1..790
                     /locus_tag="U4B29_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2107706.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="U4B29_002818"
     CDS             887..3136
                     /locus_tag="U4B29_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2107707.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..4314
                     /locus_tag="U4B29_002819"
     CDS             3139..4314
                     /locus_tag="U4B29_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2107708.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4317..>4548)
                     /locus_tag="U4B29_002820"
     CDS             complement(4317..>4548)
                     /locus_tag="U4B29_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2107709.1"
                     /translation="KNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGL
                     ESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1576 a          657 c          829 g         1486 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagtaaccc gtggtgctag ttaattttaa
     4321 tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca
     4381 ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg
     4441 aaattttgac aacctaattt ttctaaggaa gaaaaaacag tctcaactgt ttttctttgt
     4501 tttccaagta agctgtggtc tagtttttct gatttatcca tgttttta
//
LOCUS       DASJTM010000153         4477 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJTM010000153 DASJTM010000000
VERSION     DASJTM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.156"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(49..423)
                     /locus_tag="U4B29_002821"
     CDS             complement(49..423)
                     /locus_tag="U4B29_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2107710.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(446..1846)
                     /locus_tag="U4B29_002822"
     CDS             complement(446..1846)
                     /locus_tag="U4B29_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN2107711.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1850..2260)
                     /locus_tag="U4B29_002823"
     CDS             complement(1850..2260)
                     /locus_tag="U4B29_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2107712.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2278..2769)
                     /locus_tag="U4B29_002824"
     CDS             complement(2278..2769)
                     /locus_tag="U4B29_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2107713.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2779..3570)
                     /locus_tag="U4B29_002825"
     CDS             complement(2779..3570)
                     /locus_tag="U4B29_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2107714.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3563..4336)
                     /locus_tag="U4B29_002826"
     CDS             complement(3563..4336)
                     /locus_tag="U4B29_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107715.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1389 a          935 c          590 g         1563 t
ORIGIN      
        1 aatgaagtac ttttcgttca taccaactta gagcacttcg ttatctattt atttcttcat
       61 ttggcttatc tctataatct catgatttgg tccgtagaaa ttaaagtaac ggattccatt
      121 ttcccagaac ggtaattcca taatattttt atctaataat tcatatccta aatcttgaat
      181 tgaattgaat acctcatcta tgtcatccgt atctaatgct agatgatcaa tggctcctct
      241 acactcagta gttttatctt cccatatttc tattatcgtt ccattcaact cgaaaaagcc
      301 gactttactt cccacattca tttcttcatt aatcagacta aaccctaagg attcataaaa
      361 gatttttgaa gctccaaaat cttttgttgg aattccaata tgttgtaaac catttaattt
      421 cacttacatc atcccttcaa ttgttttaaa atccacattc ataatttatt gaatagtcta
      481 gtttatctac cttgattgct ccctccgtaa cagtcatact taatgctgtt ccgactttat
      541 catacattct agtacttaaa gcaacattat caatatataa aacagctatc gtatcatcaa
      601 caattaactt tagttcatat tctttgccta tttccaaaaa tagtggtcgt tgcagcccct
      661 tatcaaacat ttgaggccaa ggatagttcg gagtttttcc gaaagtaagc tgattattaa
      721 tcaatgaaat acgaaattcg tatccttctt gagtatcact atctacaaat aattttaaag
      781 caaactcctt tgttaacgat gtaatagtca aagttgtttc aaatagaaaa taatcttcga
      841 ttttaggaac aattactctt tctttttttc caaattcttt ctctaactta aaacttgaaa
      901 atggagtgat ttctttaaaa ttctcaatta ttttttcagg cagtttaaca ccaagagttt
      961 tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc gccccaatcc cagttatttt
     1021 gatcgttctc cccttctttt gttgccaccc agccagttag aaaacggcca gtttcattag
     1081 ttccgactgt tcttgctgca taataggctt ttccgtcgaa agcttcgtct actggtgatt
     1141 tccaatctga aaataatcct gctccgactc gatatttagt ctttttatct tcacaatact
     1201 ctgaaaaaag taaataccac gttccatcaa tttcaaatat atcaggcatt tcaatcatgt
     1261 taaattcatt attagcccag tagtctcctt tgaaattcca atcttttaaa tcttcggatt
     1321 caaaatagac caatctcccc gtcggctttt tcttacttgc tggttttcta gctcctagta
     1381 tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa aggatcgcgc caatcattct
     1441 tatcatatcc ttcttgagga acaagttttg ctgcttcgcc atcctttttc caatgaatta
     1501 aatctttaga acttgcaatc attaacactt ctgaagtttt tccatccaac ttagctttgc
     1561 gattatgtcc agtatacaaa gctaaatact cattgattcc ttggacaata cttccagcgt
     1621 aaatgtattg atcaatgtca tcgttgccac cacgatgaat tacttccccg tattcttcaa
     1681 atttcacaaa atcagtggtt gtaactagtg accacccaaa aggatcagtt aaaggtttag
     1741 gatttctcgt atctcgttga tgaaatagat aaaaagttcc atctttataa aaaggcatgc
     1801 aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt ttgcatttat cactcatcct
     1861 cttcgttagt atgtctttgc atatctgcaa gacacgcaat tgtattcata tttcttagat
     1921 tttcgatgat atcatcgctt ttttttcctt gtaatctcaa attgacagcc tcaatcaaaa
     1981 ttcctaaatt catgccacca agaagttgaa acccattttg aaatacttca cttcttttag
     2041 caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa taccataact tcttgatttt
     2101 ttacttgaga aactattttt tcaatgtttt cacggaattc ttcaatgcct gcatctccaa
     2161 gtgttaccac atgacaattt aaattatcgc caaaaaacat ttttaatgta tctacaaatc
     2221 caatggcaat atttccatga gatgtaatta taaactccat ccactcatcc tctgttctta
     2281 ataaataaaa tcctttaaaa attcttttga atctctagga gtttgtatca ctgtcacttc
     2341 aactccatca ctaattagtt ctttaaaagc ctctctctct tcttcagtaa tatttatcgt
     2401 cggacggatg cgttctgttc cattccttct tgataaattt cctacgttta cacttgttat
     2461 tggtaaaccg ttatcaatca aaaatttaac atcgattgga gaaactgcaa caataagaac
     2521 tttttgtcct tcatatttac cagaaagaat atttttaact gctacttccc ttgtaattat
     2581 tgaagttcta acattaggtg gtgcagcgag gcgaactaca gcttttttta cgtcatcatt
     2641 ggctacttca ttattaataa ccataattct gttagcactc aattgatttg tccattgtgt
     2701 tgctacttgt ccatgaatca ttcgatcatc aatacgaaca tgtataatac ctttcatttt
     2761 ttcatccata ttcaacacct acttcaaaat atttaacgct cttaacgcaa tagaaactac
     2821 taagattaat agaattacat aaactggttt aacctttcgt cctaacaaat aatacacaat
     2881 tgctactaca catgcaggta ataaaccagg cataacttga tcaaagattt catttccggt
     2941 cattgttaag tcgccttgtt taaattcaaa gccaacattc acttttacaa ctgtggcaat
     3001 gagtgcaccg accaccatta accctaaaat tgatactgca tcagtaatag actttaattt
     3061 tgtttcaatt tgagttagta ggctcgcacc cgattctttc ccagcaaggt ataaaggata
     3121 agcagtaatc cttaataaaa ttgatgccag tatccacaaa ataattccaa caaaactacc
     3181 ttccaatgcc atattagctg caattgctcc aaaaatagtc catggaataa ctacaaatac
     3241 actatctcca attcccgcta aaggacccat tagacctgtt ttcagagcac ttactgcttc
     3301 caaagactta tttttttctt ctttttcaat tccaacattc ataccaataa tgtaaggcgc
     3361 tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa acggattgtt tcattccttc
     3421 aggatcatct ttatatgtgt ccttgataaa aggaagcata gaatacgcat aacccaaccc
     3481 ttccattttt tcataattta atgaagttgc accattgaga atccaccgga aaattacttg
     3541 tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa acctcctgtt gcagctggtg
     3601 ctacttgagt attatttgat ctatttttat atacgattag tgcgattgca gcgccaagta
     3661 acgaaatccc taaaattggt atttggaaat aaactgccaa aacgaaacca actaacaaat
     3721 aggcaaagta atcttttgta ggtaaatatt taagtagcat tcctatccca acagcaggta
     3781 atagtccccc tgctaccttt aaaccaccgg taacccattc aggaatataa ttgattgcac
     3841 ttcccactaa cgttgggcct aatatcacag ttaacaaaac aggaattcca gatacaaagc
     3901 ttgtattaat tgatgccaaa tattgcatcc actctaattt ttgataatct tctttttgtg
     3961 cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga ccactttaga acatctagtt
     4021 gtacaattaa cattgctacc ggtattgcta aagttattcc tatagaagca tcctggttag
     4081 ttgagattgc taaataagta ccgatcaatg ctgctgtttg ataatccggt actgatgcac
     4141 caccaaaact agtaatacct agtgtcatta attgcaaagt cccaccgata taaagtccag
     4201 tttgaatatc tcccataata agtccggtca agaaacctgc aacgacaggt tgggaggttc
     4261 cgattttggg cccttcctta tcccagttaa tgaaaaatcc atacaacaca actaaaaatg
     4321 tttgccaaaa cgccattttg ttctctcctt tcttcttata cttttatata agcaattact
     4381 gtgccaacta acattatcca ataaagcggg aaaatacagt aaatattaat taaatgagcg
     4441 tctaaatata ctttggacac cctttcattt ttttaga
//
LOCUS       DASJTM010000154         4425 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJTM010000154 DASJTM010000000
VERSION     DASJTM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.157"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="U4B29_002827"
     CDS             690..1658
                     /locus_tag="U4B29_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2107716.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U4B29_002828"
     CDS             1669..2484
                     /locus_tag="U4B29_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN2107717.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U4B29_002829"
     CDS             2552..3262
                     /locus_tag="U4B29_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107718.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="U4B29_002830"
     CDS             3298..>4425
                     /locus_tag="U4B29_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN2107719.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASJTM010000155         4282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJTM010000155 DASJTM010000000
VERSION     DASJTM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.158"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="U4B29_002831"
     CDS             complement(37..1257)
                     /locus_tag="U4B29_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN2107720.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="U4B29_002832"
     CDS             complement(1277..1888)
                     /locus_tag="U4B29_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2107721.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="U4B29_002833"
     CDS             complement(1931..2863)
                     /locus_tag="U4B29_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2107722.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="U4B29_002834"
     CDS             complement(3284..4144)
                     /locus_tag="U4B29_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107723.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DASJTM010000156         4277 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJTM010000156 DASJTM010000000
VERSION     DASJTM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.159"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..991)
                     /locus_tag="U4B29_002835"
     CDS             complement(<1..991)
                     /locus_tag="U4B29_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2107724.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIK"
     gene            complement(1099..3126)
                     /locus_tag="U4B29_002836"
     CDS             complement(1099..3126)
                     /locus_tag="U4B29_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2107725.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3271..4002)
                     /locus_tag="U4B29_002837"
     CDS             complement(3271..4002)
                     /locus_tag="U4B29_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2107726.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1180 a          893 c          709 g         1495 t
ORIGIN      
        1 ttttaatcgt aacatccgtc gtgttcaatt ccttggctac ttttccgtct attgtaaaat
       61 ctttttcaaa tacccattga ttggtatagt tgatcgtagt atttcctttt tttctcgaaa
      121 attttccatt ttcattttca tttcctaaaa cagtcattgt aaggtgatat tcacctgggg
      181 ttagcggttg accatttaaa gcagttggaa aagaaaaatt tgtattcggc gcaatctgca
      241 atccttcttt ttcctcttga tataaaactt cagaatgtcc tttttttgta atttttgttt
      301 caataactac ttgattgata taggtttttt gatcattttg aacgtttgct aagatgacat
      361 ttcttgcatt gatttgatct ggtttaactt catgaagagt aaggttcgga gcaacttcgt
      421 tcagtgtctg ttgcatcaac aaagcaataa tataggtgta ttcattctca atcgataagc
      481 tttgatcctt agtttctgtt tggtcctttt ccgtctggat ttcttggaat gtgatcccac
      541 cagccaatac accatcaaat cgctcattcg gcatcctgac atgaaaagca acggtttgga
      601 cacttttagg ttttagcgtc actatctgtg gatagctcac ataatctacc agatcaaaga
      661 tcaggctcat gtctttttca atctcgtttt ttccgtattc aaccatcaca tttgaattgg
      721 tggttgcgct attcacagaa atatcgattt tcacttcttt ttctgtatca tttctcaatt
      781 ccgcttttac ttctacttcc tgatccggat caagttgaag atcgaagtaa gtttttctct
      841 tgtcgatttg attctctgaa gttatcggcg tcactgaaaa attaaatgca ctagctgaca
      901 ctgatatatt gttaaaaagt gcccctaaaa taactaggag tgttataaat aattgttgtt
      961 ttggtctcaa gaaaatcgct cctttaatca tatgtaagag aagactataa caaaatcttg
     1021 tctctgtggg acacaagagc ctatggtacc tgtgcccttc ttgaaactca ataattttgt
     1081 tatattcttc tcttcctgtt aatttcctgg gacatctgca agtatccaag atagtttccc
     1141 ctcaaaggtt ccgattcttt ggttttctac aggaatattt attctgattc cacgatgagc
     1201 tgtagaatct aaaagagagg acaaatcgct ttcacccttt tgatcacttt cgaaaatcgt
     1261 aacagcggat gtccctataa ccgttgtatt ttcagaggta acaaaggaaa gagcttctgg
     1321 aagctcaaaa tctccatttg taagaacttt ctcttgtttc aattgtaatt tccaacttcc
     1381 aaaagcccgt tcatctataa ttttcaaccc acctgaaaac tgtccgaatt cacttaatgg
     1441 tctactggat attttatgtt ttccaaaatt gacatattga ggagcagttg agagataaag
     1501 tttgcctttt ctgtaataaa aatagatatc ctctgtattt tcggagaaga tgccttctgt
     1561 attcgctgaa tccgagtcaa aaacataccc ttcaatatca ataggagttt ctttatactt
     1621 ctctcctatt ttcccagtga ttgttttcat ttcagcaata tcaatctgac tgtctttatc
     1681 tttataataa acatggatat ctatctcctt cagctttgcg atctttaggt ttatttcatt
     1741 cgattgctca atcaaattcc cgttttcaag gtattcaaaa tgcatagtga gtactttttc
     1801 aggaattact tcctgcaaag ttgtctccaa ttcgattatt aacgtatctc cagcaataaa
     1861 ataatctgtc agatttagcg tgactcgatt tccggttatc aagatatctt tggcatcaat
     1921 ttgatttcca ttgatcaaga tatgctcggg aatagttaat tcttgtggga ggtcgatact
     1981 agtctgcatt ttcttatatt caagaaaatc cccctcgacc ttcaatgtat ttctgatagt
     2041 tatcttatcg tttcctccag cttcagcgat ggccccataa tcactatcgt tgacttttac
     2101 atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca gcgacagcat tttcttgaaa
     2161 aaaagtacct gttgaagaag taaaccccca atttacttct gtagaaccaa actgcctaat
     2221 gagatcaggt ataatccgag aagagcgacc gacaccttcc aaatcataag taagacttct
     2281 ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg ccattagaaa gaaatgtagg
     2341 agctaccgtc acgccagtat gctctccaga ataattatta ttatttggtt taggcgtgac
     2401 aaaagcgaca tgtccatgcc ctctattatc agcagttatc tctcgatcca ttcgatcaga
     2461 agaaccacta tttttatacg tatcaaactc aatcgagagc gcattcgcaa tgtaaggttg
     2521 gccagaacga gtagaatatg agccaattcc catacctggc gatccaataa cttgactggg
     2581 attactagcc attctaggat cgccagtcaa tgtaaaggtc atgccatctc ccgctctatc
     2641 tcgttgattt cctaagtata aataagattt aaatgtaaaa tccgagagta aactaatctt
     2701 cttatttgac caaatagctc ctctttgaga cctttctgct gggttcaact gcagtaactt
     2761 cccattttcc aatatggacg tgctagtacc gataggtgtt tgaaaaattt tatcgataga
     2821 aataatattg ggatcgtcag gcagctcaac cgcagaaatt ggtgcatctt ctagtccttg
     2881 tttgtccgaa ccctcagctt ctacgttgtc agtggtctct ccactactcg aaatacttaa
     2941 atcctcactg ctttcttgtg tctggttgga ttctattaaa ctgtcgaatg tcgaagtgag
     3001 tttgctttct tcgtctcttc tatttccatg ctccaactga ttcaattcca aagagtaccc
     3061 tatgatcgga aagatattag tcactaagag actgcttaaa atgagcacga tcctcctctt
     3121 tttcaatata tcttttcctc ctgatcgtta aattcctaga tgaactattc gtatccaaac
     3181 aaatttagcc tagcctttga caatttttgc tcgattacct cgcaagacaa tatgactacg
     3241 tatttatttt taacagttct tttcagtaaa ctagttgtta ggtgtatctt ctaatgtcca
     3301 aactaattta gtggaatatt gttgcgctaa cttgacagat ttaccgggta ccgaaagctg
     3361 taccgcatct tgattttcat tttcatttgc accaaatcga tagacccaag tccccacacc
     3421 tgttcctgat ttagcattaa ttgctaacgt attgtttcct cccggtgtca acgttaattc
     3481 agaattcacc gtttgcggcg tatatgttgg atcaaccaaa gaaacagcct gccctttcgt
     3541 aaatttcagc tgtgctccaa ctagttcatc cccactggca gttttaaact gttctggttg
     3601 agagacactt aatttccacc cagataaggt acttctccta tctgtcactt ggacatatgt
     3661 cggttttcta gacccatcgt tcatcacatc agcagctgca taatacgttt tatccgcagt
     3721 agaaattttt tgagtcccaa atttgaattt tgatccataa tcaatcgata atgcccctcc
     3781 tgttccaggg cttggaggat ctgatggatc gacaggttta ttgggatcag gattctctgg
     3841 atcaacagga ggagttacac cctcatctcc tgcttcaaat gtaatcgttc cttctgtgtt
     3901 atattccttt gggtatactt cagttgcaga aacaaaagtc gtacttgcta ccatgctgaa
     3961 taacatcatt gctgctgtta atacgatttc ttttttcatc attgacattc ctctttcttt
     4021 atttactaaa catatcagtc gattggatta atccatagca aaattctgtt ttacgatact
     4081 tatgaaataa aagaacatag acctatattt ggtcctatgt atcgattagt tataaatgat
     4141 tatcagcccg atttgatttc tagtttttat tgcaacttga tgcaaacaca atctagaaat
     4201 acagctgtca tagcctttaa aataattcgt aagtccccct cctatctatc aaaatgtgac
     4261 ttgacgagag tgtaaaa
//
LOCUS       DASJTM010000157         4233 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJTM010000157 DASJTM010000000
VERSION     DASJTM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.160"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..503
                     /locus_tag="U4B29_002838"
     CDS             75..503
                     /locus_tag="U4B29_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2107727.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="U4B29_002839"
     CDS             490..732
                     /locus_tag="U4B29_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107728.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="U4B29_002840"
     CDS             complement(1032..1247)
                     /locus_tag="U4B29_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107729.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="U4B29_002841"
     CDS             1354..1668
                     /locus_tag="U4B29_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107730.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="U4B29_002842"
     CDS             1772..2950
                     /locus_tag="U4B29_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2107731.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="U4B29_002843"
     CDS             3353..3646
                     /locus_tag="U4B29_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN2107732.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1448 a          634 c          821 g         1330 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttt
//
LOCUS       DASJTM010000158         4176 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJTM010000158 DASJTM010000000
VERSION     DASJTM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.161"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..1338
                     /locus_tag="U4B29_002844"
     CDS             37..1338
                     /locus_tag="U4B29_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2107733.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1365..1625
                     /locus_tag="U4B29_002845"
     CDS             1365..1625
                     /locus_tag="U4B29_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2107734.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2624..3859)
                     /locus_tag="U4B29_002846"
     CDS             complement(2624..3859)
                     /locus_tag="U4B29_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2107735.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1207 a          670 c          869 g         1430 t
ORIGIN      
        1 gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat gaaatgggtc
       61 gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt attagcaatt
      121 cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt tatggtcttt
      181 atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc aacgattgtt
      241 ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt tatgggttcg
      301 gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc tttaggaacg
      361 ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc tcttggctgg
      421 gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt atctatgatt
      481 tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa aatgccagaa
      541 caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc agtcatttta
      601 acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc cttattatcg
      661 ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt actaccaatg
      721 tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt aggggctgtt
      781 gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc agatggtaca
      841 gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac ggtaacaatt
      901 ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg taaatcaaaa
      961 ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat caatgagccc
     1021 ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt caacttagtt
     1081 cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt caatagattt
     1141 actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc agcaggaaat
     1201 gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat tatctattat
     1261 ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg tggagaaaac
     1321 gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat tggctgattt
     1381 tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat atgctagcta
     1441 catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg atcgtgattc
     1501 ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca tatgtacttt
     1561 gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc acatcgaagc
     1621 tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat aagaagaaat
     1681 ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt ttggttgaac
     1741 ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga ctgtgacaag
     1801 gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt tttggcagaa
     1861 agaaggagga aaaaatggaa attttatcag caatggattt agaattagta gatctactac
     1921 caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag gggctgcagt
     1981 atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg aaacgtgtat
     2041 agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt agtgtatcgt
     2101 tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt gctggcagtt
     2161 tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg gaggttgatt
     2221 atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg gactagcggc
     2281 acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga aaatgtattt
     2341 gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc agagaaaggt
     2401 gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt ggatagcgtc
     2461 agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta agtaactcta
     2521 acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc gaatagcgaa
     2581 attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt gggtggttgc
     2641 tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga aaaacggctt
     2701 gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga gcaatctgca
     2761 gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt acacaatagt
     2821 caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg ccggattcac
     2881 aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg aattcatgca
     2941 aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat tgaatgtcat
     3001 caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt aagaagaaca
     3061 aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag ataatacgtt
     3121 ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag gaagtactga
     3181 gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct tcttccaaaa
     3241 atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa cttgtttttg
     3301 gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc catcgggtat
     3361 ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg aagaaacgta
     3421 actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa gcatagggtt
     3481 taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc acattcgaac
     3541 ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta gtgatacgag
     3601 aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca aattttatga
     3661 tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca aagccacttt
     3721 ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa taacggtctt
     3781 tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat actgcaggag
     3841 caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc ttcgtttctt
     3901 actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa tattaaactg
     3961 tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga aactccaatt
     4021 ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga taaatacgag
     4081 aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataatt atattaattg
     4141 ataggtgtgc tgtcgtcatt atacacacgt aagaaa
//
LOCUS       DASJTM010000159         4093 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJTM010000159 DASJTM010000000
VERSION     DASJTM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.162"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..153)
                     /locus_tag="U4B29_002847"
     CDS             complement(<1..153)
                     /locus_tag="U4B29_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2107736.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="U4B29_002848"
     CDS             complement(475..696)
                     /locus_tag="U4B29_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107737.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="U4B29_002849"
     CDS             complement(794..1039)
                     /locus_tag="U4B29_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107738.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="U4B29_002850"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="U4B29_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="U4B29_002851"
     CDS             complement(2263..3264)
                     /locus_tag="U4B29_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN2107739.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="U4B29_002852"
     CDS             complement(3423..3689)
                     /locus_tag="U4B29_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107740.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="U4B29_002853"
     CDS             complement(3679..4035)
                     /locus_tag="U4B29_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2107741.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1295 a          744 c          542 g         1512 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga att
//
LOCUS       DASJTM010000160         4077 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJTM010000160 DASJTM010000000
VERSION     DASJTM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.163"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(186..1691)
                     /locus_tag="U4B29_002854"
     CDS             complement(186..1691)
                     /locus_tag="U4B29_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2107742.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1688..2374)
                     /locus_tag="U4B29_002855"
     CDS             complement(1688..2374)
                     /locus_tag="U4B29_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2107743.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2567..3988)
                     /gene="gndA"
                     /locus_tag="U4B29_002856"
     CDS             complement(2567..3988)
                     /gene="gndA"
                     /locus_tag="U4B29_002856"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN2107744.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1124 a          860 c          695 g         1398 t
ORIGIN      
        1 acgaatatta cagcatttta ttgttttttc gttcatctaa aagatactta catttaatta
       61 acggatgtta ttctatttaa ttgttcgcta gtttttttat aatgacatcc ctgaatttct
      121 cacttgcttg tattttgtac cttattcttt gttggatata taaaaaaaca agtaaagcac
      181 atgctttact tgtcttctgc ctttgcgata ggaataaaga ttctaaaaat tgtcccttgc
      241 cctactacac tttctgctaa gatacgtcct ttatagtttt caaccagttg tttagcgata
      301 gacaacccca gaccatttcc acccttcgtt ctagctcttg cattatccac tcgataaaaa
      361 cgattgaaga tcttattaag gtcttctttg ggtattcctt cgccaaagtc ctgaatcgcg
      421 atctcaaatt cattcagagt ggaagaaata gagatatgga cttcttttcg tgtagttgaa
      481 tactttactg cgttatccaa tatgataatg attaattgtt caaagtgatt acgataaatt
      541 tgcagggtca cttcttttgt caaatcatca tctaaagtga tcacatagtc tggatataat
      601 atcttgaaat tattataaac ttgataggtg acttcttttc ctttagttgt ttcattcgca
      661 taatgaacat ctacttgttc tgctcttgat aagtctagca tctcttgaac taagcttttc
      721 atacggctga tttcttgcat gctggcgctt aatgattctt ctaatatctc aggatcatct
      781 ttcccccaac gatttagcag actaagatgc ccttcaataa ttgcaacagg tgtccttaac
      841 tcatgagata cgtcttccac gaattgctct tgttgttcaa tatacaaacg catacggtca
      901 agcatctcat tgaatatttc agctaaatct gccaattcat catttgtgtc gatttcaggc
      961 atgtggatat ctgattgcgg atctttacgt atagtatcca ttgtatctct gagtaccttg
     1021 agtggtttta agaaataaga agataaaaag aaacctaaaa tactacttaa gatcaaagaa
     1081 acaacttcta aaacaataag agtcaataat agcttatttc gaatatcata gaaagaagat
     1141 aattcataga agctttgcgc atagccgatc ttttctcttg tacctttaga atatataggc
     1201 tggatagata aaaagccagt ttttccttct agtgtaacga tcgttggaac tttacgtgta
     1261 gtctgaatca ggtttcgact gttttcatgg gttttaaaaa tcaagttctc gtccaaatca
     1321 taaacataaa gatccattga tggctgccct agctcagcta taaaggaatc gatcttcatt
     1381 aagtttcctt cgaccagtga agtatgatct acgacctcat ctccgtcatt tgtcgctttc
     1441 gtcagattac gatacgtatt tactaatgtc agttcttcat ccgagttagc caaacgagaa
     1501 gctacttctg aaatcgtacg ctctacattg tttctttctt ttgccacaat cagattgata
     1561 gatgatttat aggtaatgac agcgaaaatt gtaaacacaa cgaatatgaa gaaagaactc
     1621 gcaaaggccc acttgattgt caaggagggc cctttcagtt ctcttttttc cgattttttc
     1681 ggctttatca cgagcgcatc acataacctg ttccgcgtac agtttggata tagctttcct
     1741 ccccgggaac gtcaattttg ttgcgaagat aacgaatata tacgtccact acgtttgttt
     1801 caacttctgt ttcgtatccc cacactttat ttagtaaaac gtcacgtgct aataccacgt
     1861 tcacgttttc cattaaagta agcagcaact cgtattctct ttttgttagt tcaattactt
     1921 catttccgcg acgtacgaca cggttttctt tttcgatagt caaatcgcga taagtaatag
     1981 tagtttgttt agccacattt ttatcgcctt caatatcgat tcgacgcaac aatgcacgaa
     2041 gacgtgctaa taattcttcg atagcaaatg gtttgacgat gtagtcatct gctccgtggt
     2101 caagtccaga tacacgatcg atcacagaat ctcttgcagt catcataatg attggtgtat
     2161 ttttgacttg gcgtacacgt ctgcagactt ctaaaccgtt caattctggg agcatcagat
     2221 ccaacaatat ggcatcccac tcattattta atgccgcatc taaaccagtt cggccgttgt
     2281 agtggacttc tgcgttatac ccttcatgct tcaactctag ctcgacaaat cgtgccaagt
     2341 tcttttcatc ttcaatgatt agaatattgc tcatttatta ttttatcctt tctattctac
     2401 tggcttttta aaaactctat cattataccg ataatatgct taaaaatcta tagtcatttt
     2461 atcatggata gcaattttaa aaaagagctg aagtctactc ataacaatcg acttcagctc
     2521 tttcgtcctc tttttatttt ctcatttcct aacagccgaa agactattat tcttcatggt
     2581 accatgagta atggaagatt ccttctttgt cgacacgctc ataggtatgt gcgccaaagt
     2641 aatcgcgttg ggcttggatt atatttgcag gcagacgttc tgctctgtat gaatcgaagt
     2701 aagcgatggc tgatgagaaa gtcggtacag gtacgccagc ttggacagcc aaggcaacga
     2761 cgtctctgac tgattgctga tattttttcg tgatttcaac gaagtaatca tctagcaata
     2821 aattttcgat ctcaggattt ttgtcatagg catccgtaat tttttgcaag aatcgagctc
     2881 ggatgataca tccagcgcgc cagatttttg cgatttcacc aaatggcaag tcccaatcaa
     2941 attcttttga agcgacacgt aattgagcaa atccttgggc ataactcatg attttactga
     3001 aatacaacgc ttctcggatt ttttcgacca attctttttt gtctcccgtg aactcaaaat
     3061 cgttcgtacg agacaaaatc ttgcttgctt gtacgcgttc ttctttataa gcagaaatat
     3121 agcgggcaaa tacagattca gtgatcaatg gaagtggcac acctaagtca agcgcgcttt
     3181 ggcttgtcca tttacctgtt cctttattcc cagctgcatc aaggatcaca tctactactg
     3241 gtttacctgt tccttcatcg tctttacgtg tcagaatgtc agcagtgatt tctatcaaat
     3301 agctgtctaa ttcgccttga ttccattctt taaagatatc agccatttct tctactgaaa
     3361 ggtttaaaat attcttcatt aagtcataag attcagcaat cagttgcata tctccatact
     3421 caataccgtt gtgtaccatt ttcacataat gtccggctcc atttgggcca atatatgtga
     3481 cacatggtgc tccgtcttct gcttttgcag aaatttgctc taagattggt gcaactagcg
     3541 cataggcttc tttttgtcca ccaggcatga ttgaaggtcc ttttaaagct ccttcttcac
     3601 caccggaaac gcctgtaccg ataaagttga tgccagagtt ggctaactct tcattacgac
     3661 gcatcgtatc tttaaagaat gtatttcctc cgtcaatcaa aatatcgcct ttatcaaggt
     3721 gaggaagtaa ctcttggatt gttgcatctg ttgccggtcc tgctttgacc attagcaaga
     3781 tccgacgtgg tttctcgata gattctacaa attcttcgat tgtatatgtt gctttgaaat
     3841 ttttatctgg gtgttcttct actacagcgg ttgttttagc ccctgtacga ttgaataaag
     3901 ccactgaata gccgcggctt tctatgttca aggctagatt tttacccata acggccatac
     3961 cgacaacgcc gatttgttgt tttgacatat gaactcctcc tacatacggg atttctccct
     4021 aatctcttac attatagctt aacacggcta cattgcaaac taaagaacaa catattt
//
LOCUS       DASJTM010000161         3922 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJTM010000161 DASJTM010000000
VERSION     DASJTM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.164"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2667
                     /locus_tag="U4B29_002857"
     CDS             <1..2667
                     /locus_tag="U4B29_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN2107745.1"
                     /translation="KKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSIL
                     ASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPN
                     QMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMW
                     LDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLT
                     NWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLI
                     MKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFY
                     LVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAAL
                     FAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSA
                     EALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSAN
                     DSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNI
                     SKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLI
                     NTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQA
                     YLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTK
                     EISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDK
                     GWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILY
                     VWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(3122..3868)
                     /gene="truA"
                     /locus_tag="U4B29_002858"
     CDS             complement(3122..3868)
                     /gene="truA"
                     /locus_tag="U4B29_002858"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2107746.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         1099 a          789 c          747 g         1287 t
ORIGIN      
        1 aaaaaaaatt taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg
       61 attccgtttt tgattttagc acttgtttat ctaagtatcg gaatctatcc tggaagcagc
      121 agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga
      181 aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat
      241 tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt gttctttttt
      301 ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc
      361 ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca
      421 cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg
      481 ctggatgcat ttttttatct tccattagtg attttgggga ttcatcgtgt tatggatctt
      541 aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg
      601 ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg
      661 tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct
      721 tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt
      781 cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg
      841 attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta
      901 ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt
      961 ttgtacggca gtcttttcgc catacttatc gcaagttttt atcttgttcc attgaatttg
     1021 ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct
     1081 ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg
     1141 ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa
     1201 cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta
     1261 gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg
     1321 agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc
     1381 catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct
     1441 gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa
     1501 aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg
     1561 ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca
     1621 agagggacaa atttgaatat ccgttataac aataatactt tgctgatgga tgggtttaca
     1681 ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa
     1741 gctggtcaaa gcggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt
     1801 actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag
     1861 acaaacttga tcaatacatt gtctggtcta aatgaacagt attttacatt ctatacacct
     1921 actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca
     1981 gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca
     2041 caagcttact taagtttgtt cccaaccaat tttgcgcaat tagaaagttc tagtgcgacg
     2101 atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat
     2161 cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta tgggacgaaa
     2221 gaaataagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca
     2281 atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg
     2341 actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg
     2401 aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg
     2461 ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg
     2521 ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt
     2581 gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaca accacgaaac
     2641 acacacagaa gaagacgtag aaaataaaga aacgatggaa cctgttcaga ctgtccgcac
     2701 caagggcaat caaaacaacg ttccatcgtt ttttggttac agaaaaaata ggtaacaaaa
     2761 agatgattca cttatcaacg cctcgtccac aagtcacgat attcatcaaa gatgagaagc
     2821 tgttttttga aattgctctt tgtgactcag agttaaatga caggctcacg acctctttct
     2881 taaacggtgt tcaatcagct gaccttcggt attagctcaa caaacggcaa aatatgagga
     2941 gctattttcc cgtttgtttc tctaatactc aggtcaaaac gctgatttca cgacctcttc
     3001 cttaaacggt gttccagaag tagcttcttc gaaaataagc cgaaattttg caaaattttg
     3061 agaagtaatt ttcgtaaata ccttcttatt tcttgaagct gaccacttct atcataacct
     3121 cttattcata tttcacttca tacaaataaa gtccttcagg gtgtgctgta ggtcctgctg
     3181 cattacgatc ttttttcgca ataatatctg ggatactatc ttcaggcaat cggccattgc
     3241 caattttcaa aagcgttcct actaagatcc gtatcatctt atacaagaag ccatttcccc
     3301 taaatgtaaa caacaattcg tcttttgttt catttacctc catcttagcc tcgtagatcg
     3361 tacgtatttt atcctctatc gagcttcctg atgcacaaaa agaggtgaaa tcgtgagtcc
     3421 ctaataaatc aggaagcgcc cgctgaattt tttccaaatc aagtgggtaa ggaaaataac
     3481 ttgcataatg gcgtcgaaaa ggactgcgtg gtttccctat atccactcga aattgatacg
     3541 tcttttcttt gaccaaatat cttgcgtgga agtcttctga aacaatttct acttgtctta
     3601 cagcaatatc ctctggtgac tgggtatcta gcgcgaaacg cattcgttct aacggacgct
     3661 cctctggata atcaaaatgg attacttgcc cgatcgcatg tactccggca tctgtccttc
     3721 ccgaaccaaa gaccgtgatt tcttttccat ttgccatttt ttgtaatgtt ttttctactt
     3781 cttcttgaac agtacgccca tttggctgtt tttggaatcc attaaaattt gttccgtcgt
     3841 acgcaatgat tgctttgtaa cgtaccatgt ttttctctcc tttgttccta aaaggttatc
     3901 tggaataatg atttttctat aa
//
LOCUS       DASJTM010000162         3893 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJTM010000162 DASJTM010000000
VERSION     DASJTM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.165"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..386
                     /locus_tag="U4B29_002859"
     CDS             <1..386
                     /locus_tag="U4B29_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107747.1"
                     /translation="LNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLL
                     FTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGI
                     LEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(512..1189)
                     /gene="nth"
                     /locus_tag="U4B29_002860"
     CDS             complement(512..1189)
                     /gene="nth"
                     /locus_tag="U4B29_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN2107748.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1196..1894)
                     /locus_tag="U4B29_002861"
     CDS             complement(1196..1894)
                     /locus_tag="U4B29_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2107749.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2162..3346
                     /locus_tag="U4B29_002862"
     CDS             2162..3346
                     /locus_tag="U4B29_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN2107750.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3352..3795)
                     /locus_tag="U4B29_002863"
     CDS             complement(3352..3795)
                     /locus_tag="U4B29_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2107751.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1013 a          767 c          638 g         1475 t
ORIGIN      
        1 tattaaataa atttacttta attgctccta tccttgcatt tattattttt tctgtacttt
       61 taagcactac tcttaaagga aaatttatgg aaagaagctc tcatgcaatg attctcactt
      121 ttttatggtt gttgttcacc agaatctata tttttctgat gagcctgaaa ccgcccaaat
      181 ccattagttt atgtttagtt ataaatggta tttttttatt atcacttatc atttttatta
      241 cacctttaga tagatacgtc acttatttat ataatccttt ggaatactgg acttatttta
      301 taggtatatt ggaaggcatt attttctaca tcgggtattt tcccaataaa aataacaact
      361 tttattttta tcgcaataaa ttgtaataag ttaccatcct agctactgag tatgtacatt
      421 tactttttca tgcaaaaaag ataaacgaaa cagcttttct ccataataac aagataattc
      481 atacatttat ttgcaattaa agaaaccacg tctacaggcg tggtttcttt tttagcgcct
      541 tttcttttag acgcatccga tttttcccat cttgacatat tgatagcaaa ggacatacct
      601 cgcacttggg atttcttgcc gtgcaatgat atctaccaaa aaaaatcagt gtatgatgtg
      661 tctttaccca taattcttgt ggtactttcc gcatcagtgt ttcttctact tccatgaccg
      721 tagcatctag tttgcagatc ctcaggcgtt ttgagacacg ttccacgtgg gtatctactg
      781 cgattgctgg tatcccgaat gcatccccca atacgacatt tgctgtcttt ctgcctacac
      841 caggaagaga catcaattct tctcttgacg ttggcacttg cccatcgaaa cgttcaatca
      901 gctgctgtgc acacgctttg atgttttttg ctttatttcg atacaaacca atcgttttta
      961 ttttaagaat gatttcatcg attgaagctt ctgctaacgc atcgggtgtc ggaaaagagg
     1021 caaacaaatc tggcgttgct ttattcactg atacatctgt cgcctgggca cttaagatca
     1081 ctgcaatcaa taactcaaat ggattattgt gcttgagttc tccatgtgct tcgggaaaca
     1141 ttccatacat cgtttctaga gcttccattg ttttttgttt gcttaacata ttccgctact
     1201 cctccaacca atttttcagt gagactttcg gcaattcttc ttgttgagct gactgttcta
     1261 cttctctttg aagaagctgc tgttttcttc gtttttggtc ttcttccact tgttgttttg
     1321 tacgtagatt ttttctttcc cacgatagca aaatgcggtc aacgtaatta aaactataag
     1381 cctgatttaa tacggcttcc cgcagtgcta gcttcaaaat ttccggctga taatggtctt
     1441 cttctagcca ttgtccgatc cgctggaact cgatcgaaga aagcgggcgt ccaaattctt
     1501 gttcaaataa ttgatacact gactggattt ctttttcttg tgttttttct tcgtattttt
     1561 tttcttgtgt ttttaaatac tcacctaata gatcatagat ggggtaaaga ttatatcgat
     1621 ctgctttttt accgttgaca atcgtcgttt ctatcttgat aaatccattt gttacgagac
     1681 gatccagtat ttgaaaaatc tgatcttgtt tgattcccat atccatagca atcatctgca
     1741 aatcagggaa agtatctcct tcaagctgtg ccatatgcaa ctgtagaaca aataaaaatt
     1801 cttcagattg tagaccgatc ctatgataat ttttaagtaa aagattagat atgactgttt
     1861 gtccagcgct taagtaattt tctaaattca acatgatttc tcctccgctc aatcacagta
     1921 tacgtaaaaa aaacatggga agcaagaaca ggtttctttt ctatttactt cactgatcgg
     1981 tcagatttac aatgtcgtct acttgatttt tgtttgttca tgtttttgat gcgtatcttc
     2041 acttttcgga ataaataaat taagtagatt tcgctgttcc ttagaatgtt gacaaggaaa
     2101 caaaattact atacactatt tttgtttcca agttaacatt attgcataaa ggagttttta
     2161 catgtacagt ttcacaaata aacattctaa agtttttgga tacgtctctg tcttattcac
     2221 tggattagga tttactatcg tcagtcctgt acttccgttt ttaacgctgc cttattctca
     2281 ctccatacat cagcaagctt tctatatcac tttgctcatg tctgtttatg ctctagctgc
     2341 cttcttatct gcccccattc tcggtagctt aagtgatcat tttgggcgtc atccgatcct
     2401 tattatcagt ttattaggat caagtatcgg atatttgctt tttggtttag gaaattcgat
     2461 atggatgttg ttccttggtc gtatcattga aggattgacg gcaggagaaa tctccacctt
     2521 atatgcttac tttgccgaca tcacggaacc taatgaaaga accaaagttt ttggatggat
     2581 gggtgcttta gtcggtatag gaacaacgct gggtcctatt atagggggtc tattagctga
     2641 gttggggaat tctgttccta tatttattgg agcactcttt actttcttaa atgccgttta
     2701 tggctacaca tttatgctag aaagccttcc aataaaaaaa cgttcagtag atttaagttt
     2761 ctcccatgta agaccttttc accaattaca acaattattc aagatttcat ccgttattcc
     2821 tttattgaca gcaggttttg ctgtttggtt ggcagctggc tctcttcaat ctatcttttc
     2881 tcaattttcg attgacgcct ttcagtggaa ggctgggttg gttggtttaa gcttctcttt
     2941 gattggaatc ttggatactg tctcgcaatt gttcattatg cctcgattgc tcaaaaaatt
     3001 ttcagaacag cagatcacaa gaatagggat gttcagtgaa atcctcgcct atctattcat
     3061 cacattatct ggaattcttc tactgccgat tttgttttta tttgggatta tctgttatgg
     3121 tttcggtgat tctattttta ctcctgtatt caatggccaa ctatccaatt ctgtctcaga
     3181 taaccagcaa ggtcttgtaa tgggcggtac ccaaagtata caatctctat cgcgcgtcat
     3241 cggcccgcta atcgcaggac agctttatgc tgcagcacca tttcttccga cggtattcgg
     3301 atttttgctt atgatttttg cctattatca ctacaataaa aaatagtagc ttcatttgct
     3361 acttcgtata aattctctgt atcgaccgat aaaacgaaat actgtgtcaa attcttcttt
     3421 tgacatattt tcaaatacag tagcatctcg ctgtacccat ttttgatgga gttgctcgtg
     3481 tctattcctt attttttgtc cgattggcgt caattgaaaa tagatttctt ttttattctc
     3541 ttcgctttga taagtgttta tgatccctct agctatcagt ttttttgtta gcttactgat
     3601 ggctcctcta gtcatgtaac tagcattcgc taattttgtc acgttaggat cagggatttt
     3661 cccgatatgt tcaatcactt ctagttcatt tagagtaaat cctgcaaatt ctttttccat
     3721 ttctggttta ttacgatgaa taatctcatt agctaaatcc agatattctt ctattagcag
     3781 ttgtctttta tccatttcta caccttcttt ttctttcatt atattgtttt tttgtttcct
     3841 attcaacaaa gataaaaata ttttgtagga aaacccaatc tgaaaccaag aaa
//
LOCUS       DASJTM010000163         3675 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJTM010000163 DASJTM010000000
VERSION     DASJTM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.166"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="U4B29_002864"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="U4B29_002864"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN2107752.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="U4B29_002865"
     CDS             complement(1171..1866)
                     /locus_tag="U4B29_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN2107753.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="U4B29_002866"
     CDS             complement(1879..2595)
                     /locus_tag="U4B29_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN2107754.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="U4B29_002867"
     CDS             2925..3533
                     /locus_tag="U4B29_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN2107755.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1050 a          756 c          659 g         1210 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agctt
//
LOCUS       DASJTM010000164         3664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJTM010000164 DASJTM010000000
VERSION     DASJTM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.167"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..277
                     /locus_tag="U4B29_002868"
     CDS             <1..277
                     /locus_tag="U4B29_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEN2107756.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="U4B29_002869"
     CDS             420..641
                     /locus_tag="U4B29_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107757.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="U4B29_002870"
     CDS             657..1499
                     /locus_tag="U4B29_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107758.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="U4B29_002871"
     CDS             1562..3364
                     /locus_tag="U4B29_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEN2107759.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="U4B29_002872"
     CDS             complement(3423..>3664)
                     /locus_tag="U4B29_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2107760.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DASJTM010000165         3607 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJTM010000165 DASJTM010000000
VERSION     DASJTM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.168"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..707)
                     /gene="trmB"
                     /locus_tag="U4B29_002873"
     CDS             complement(45..707)
                     /gene="trmB"
                     /locus_tag="U4B29_002873"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN2107761.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(777..1559)
                     /locus_tag="U4B29_002874"
     CDS             complement(777..1559)
                     /locus_tag="U4B29_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN2107762.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1763..2974)
                     /locus_tag="U4B29_002875"
     CDS             complement(1763..2974)
                     /locus_tag="U4B29_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2107763.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(2967..>3607)
                     /locus_tag="U4B29_002876"
     CDS             complement(2967..>3607)
                     /locus_tag="U4B29_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2107764.1"
                     /translation="IGLNGAGKSTTIKHIIGLLEASKGTIEINHLSLKKNTEEYRKQI
                     GYIPETPSLYEELTLREHIEVTAMAYDIPMDIAMKRAEKLLETFRLSNRLDWFPANFS
                     KGMKQKVMILCAFLIEPSLYIIDEPFLGLDPLAINALLELMVEMKKEGAGILMSTHIL
                     ATAEKYCDRFVVLHNGEIRADGSLEMLRKEFQLPDSSLDEIYIALTKEQAHE"
BASE COUNT         1104 a          791 c          582 g         1130 t
ORIGIN      
        1 ttatttatgg atattaatgt ttttatgaaa gcagaaagtt agtttcacag acttttcttt
       61 ttggtcttat cttgaaattg agcttctact cgataaatac gttgaccttt tgaagaaaat
      121 ttttcttcat actccgtcat aatattgcct tcaaattgac tttgatgtaa atctaaccat
      181 acctgtttga ggatcatgcc atattgagaa aaacttgcca gagaatactc aaacaatcct
      241 tgattatctg ttttgaaatg gatttctcca tttggacgaa gaatctgttc atctacagca
      301 agaaaagtct tatatgtcaa tcgccgtttt tcatgacgtt tttttggcca tggatcggaa
      361 aaattcagat agatttgatc tacttcgctg tcagcgaaat actctgtcaa cgctgatcca
      421 tccacatgta gaagtttcag gttaggaagc ggttgttcga tcaacttgtc tagtgcaatc
      481 gaaacaacac tgacctgcat ttctactcca atataattga tttcaggatg ggcttttgcc
      541 attccagtga tgaactggcc tttccccata ccaatctcaa tgtggattgg atgatcgttt
      601 tcaaacaatt cattccattt tcccttccac aatgttggat cactaattac aaaattcggg
      661 tgagccgcta acatttccgc tgctcccgga cgatttcgga cacgcattat ttatctccat
      721 tcctttcctc taattaaaaa ggtctggcca aatctccgca gagtttgggc caaacctcag
      781 ccactgaaaa ttcgttttaa ttgtaaaatt tcttcgttca tccgtcgatg atcttcacgt
      841 ttgcaatatc tggtgatttc ttggagcaaa ttcatggccg cataccaatg aaatttttca
      901 agcatttcgt cagtcgggcg tatgccataa ctgatcagcc actgattcca gctagacagt
      961 ggcacataat ttcctaaaat cgttgcaata tccaccgcag ggtctgcaaa catgattgaa
     1021 tcccaatcga ctagatataa ataattttta catactaacc aattacgatg gtttacatct
     1081 ccgtgtacta ctgataagtt ttccttatct attgacggga tctgctcacg aagaaaacga
     1141 tggacaattt gcagaaattg atttttctgg agttcttccg gtaattcttt ttcatatttt
     1201 tgcagcatct tctccggcgt catgacacga ccaccgatac gggtcagcat ggttttaaga
     1261 gaatttgaat gatgtaaatg gtacaaaaca tcaatcacgt cgtttctttg accaatctca
     1321 ttaggagtca gcaagcgacc ttccagccac tcttgtgctg tcaaagtatc gccattcccc
     1381 gttcgttttg tccaaacaag ttttggtgta attccttccc ttgaaagagc agccaacatc
     1441 ggagtcgtgt ttcgtttaat aaagactttg tcctcatcac gcatgcctat gtatgttttt
     1501 cctgtgtcgc ctttgatggg acgtaaccgc cactcttggt ctaactgaaa ctccatcatg
     1561 cattctcccg tcctcttact ctttgttttg cagtacttat tattctagtc gcctgaacac
     1621 tacacgtcaa gcacatatac gttctttttc atttaattgc cattcaaaaa accagctttt
     1681 ctcaaaaaaa atagaacgaa cagcattctt actgtcaacg ctctattttt cattctcatt
     1741 tttaccctac acagctttgt tttcagtctg acatcttctt caaacgagtt ggtacatata
     1801 gtttaacaaa gagcatagta acaatcaaat aagccgctag cggaacccat cgctgctgcc
     1861 catctaatac gactgcagca atcaaaccaa aaataaccga cgcgataatt aatacccatg
     1921 tgagtagata ctgaatcgcc tgcttcttct gattcttatg gattggataa agctgaacga
     1981 gaaccatata gcggaactgg ttcgctaaag gtaacagttg gaaaccgatc aaatataaaa
     2041 ataagaaacc gataatcaga ctaaaataaa agtcgactac tccggcaatc aataacccac
     2101 ctataattac taatcggaaa taaagaccac taaaatcaga acctcttgcc aatcttcttg
     2161 caaacaagta aagataagtg ttttttgatt ctcttttaat atgagtcaat ataggatcca
     2221 aatagcgtct tcttttgatc tttgccgtaa tttcaggaac atctgtgaat aaattgataa
     2281 attgatagat ctgatgcaac ctatgttctt ctacatgcac cattttctcc caatccaatc
     2341 ctttttcttg ttgcttccac aaaaaccagt aatatagact tgcctgtact aatgcacctg
     2401 ctattcctgc ccatattagc caataaaatg atagaaacaa aaagatcaga gaagaaaaaa
     2461 accagcctaa tttccaataa cgaataactt gttgatctag tcgaaacaat tctgctctct
     2521 tgatttgcag atgactgaat ttcaaactcc ataatatgat cagatagacg aaaaacatcg
     2581 aaaaggattg ctccgtacta accaccacta atggcatggc caatccgcat gccaatatca
     2641 aaaagatcaa cggaaaaatg caagaatagc gaaaagctcg ttccaaatag gtttgcattt
     2701 ctttttcttt aggaagtaaa aaaacaatat ctgctgcctc tgcaaaagta gccatcttac
     2761 ccacgaataa acagagcatc caaaatacag agattatgag ccctccaatt gcaaacggtg
     2821 gctgcagggt tttcaaccaa ttagaataat ataatcccac accgccaatc aagaaaagac
     2881 aaactaaaac aaaatgatcg ttcaatacat aacgcatata tttcatcatt ttcttttgat
     2941 gtcttgctaa acgtttttga aaaaaactac tcatgtgcct gctcctttgt taaagcaata
     3001 tagatttcgt ccaaagaaga atccggcagc tgaaattctt ttctcaacat ctcaagagaa
     3061 ccgtctgctc gtatctctcc gttatgtaag accacaaaac gatcacaata tttttctgca
     3121 gtagctaaaa tatgcgtaga catcaaaata ccggctcctt cttttttcat ttctaccatc
     3181 aattcaagca atgcattgat agctagtgga tccaagccta aaaatggttc gtcgatgata
     3241 tataaagaag gctcgatcaa aaaggcgcat aagatcatca ctttctgttt cattcccttt
     3301 gaaaaattag ctgggaacca gtcaagacga ttagataacc gaaaagtctc caacaacttt
     3361 tctgctcttt tcattgctat atccataggt atatcgtagg ccatcgcagt gacttcgata
     3421 tgttctctca atgtcaattc ttcatataaa gaaggagtct ctggaatata cccaatttgt
     3481 ttgcgatatt cttccgtatt tttctttaag gatagatgat tgatttctat cgttcctttt
     3541 gaagcttcca gcagaccgat aatgtgcttg atggtcgtac ttttccctgc tccgttcagt
     3601 ccaatca
//
LOCUS       DASJTM010000166         3565 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJTM010000166 DASJTM010000000
VERSION     DASJTM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.169"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..116)
                     /locus_tag="U4B29_002877"
     CDS             complement(<1..116)
                     /locus_tag="U4B29_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2107765.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAY"
     gene            complement(190..1269)
                     /gene="uxuA"
                     /locus_tag="U4B29_002878"
     CDS             complement(190..1269)
                     /gene="uxuA"
                     /locus_tag="U4B29_002878"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2107766.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(1272..2903)
                     /locus_tag="U4B29_002879"
     CDS             complement(1272..2903)
                     /locus_tag="U4B29_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2107767.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            3213..>3565
                     /locus_tag="U4B29_002880"
     CDS             3213..>3565
                     /locus_tag="U4B29_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2107768.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVS"
BASE COUNT         1140 a          705 c          490 g         1230 t
ORIGIN      
        1 aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt tgcaaattta
       61 tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa aaacatattt
      121 aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta aataaactcc
      181 cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat ttaaataaac
      241 tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc cccaaatcat
      301 ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg tttttaatat
      361 cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa aatcgagtcc
      421 attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat cccgttgaca
      481 aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac ttccagtaca
      541 taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta aatcgtcacg
      601 atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg ggtgaactgc
      661 cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat atgcaaagtt
      721 ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct taatttgtga
      781 taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt ctacttctct
      841 aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg tagaagaccc
      901 atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca taaaattata
      961 acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat aattctcaat
     1021 gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa cactctcaat
     1081 aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa ttcgttctct
     1141 cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt tcattgctgg
     1201 gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc atctaaaagt
     1261 catttccata gttactttcc tccaacttct gttatatatt tattgattgt tttacgaact
     1321 gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc taccccaact
     1381 tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac cgcacaatga
     1441 atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg caactctacc
     1501 aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc cagtaaataa
     1561 cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt tccgtctttt
     1621 tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga agtatctgtt
     1681 gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt aattacttca
     1741 tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa accttctaaa
     1801 taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc tgaaattgat
     1861 tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt caagcttgtc
     1921 gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat cacaccagcc
     1981 ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata atgaactacc
     2041 tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat atctaaatcc
     2101 tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg cgtaattcga
     2161 tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc aataaatttt
     2221 tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact atcttgaaaa
     2281 cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa ctctccagca
     2341 ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg attgctaggt
     2401 ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc ttttaaactg
     2461 taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc aaaatattta
     2521 accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc agccgttact
     2581 gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa aatgtcattg
     2641 ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca cacaactact
     2701 cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg aaatcctcga
     2761 taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc ttgcttaact
     2821 tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc gatagaattt
     2881 tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta gttttgatac
     2941 attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat aaacttgctt
     3001 ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata ctccttcttt
     3061 ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt tatttttata
     3121 actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa aatatcagtt
     3181 aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa aatcaagctt
     3241 accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa aaagtatcag
     3301 aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa gcactgattc
     3361 aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat atctcattaa
     3421 ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa cgacaaatta
     3481 tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa actataattt
     3541 cagaacaaga aaaagctgtt agtca
//
LOCUS       DASJTM010000167         3545 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJTM010000167 DASJTM010000000
VERSION     DASJTM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.170"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            176..1147
                     /locus_tag="U4B29_002881"
     CDS             176..1147
                     /locus_tag="U4B29_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2107769.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1179..2576
                     /locus_tag="U4B29_002882"
     CDS             1179..2576
                     /locus_tag="U4B29_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2107770.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2735..3358
                     /locus_tag="U4B29_002883"
     CDS             2735..3358
                     /locus_tag="U4B29_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107771.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1268 a          570 c          768 g          939 t
ORIGIN      
        1 aaatgaaccg gttcaaatac ttgtttaagt ataattaata gtgagaggaa gttgctctac
       61 tgaagacttt agttatatgg atattgatga caagcaacac tatgcaaaga atgcggtcat
      121 cataatcgaa caacaagaaa gagaaatcaa atgtaactgg caggagtgag aatgtatggc
      181 atcgatccgt gatgtggcta agatggctgg tgtttcagtg ggaacagttt cccgttactt
      241 gaatggtcag caactgaagg aaaagaacat gaaaaaaata gcagaagcga tttcagcgtt
      301 ggactataaa gaaaacatca ttgctaaagg gttgaagaat aatcgaagct tctctattgg
      361 acttttgata aacgggattt ccagccgttt tgggtcggaa gttgtctcag ggatcgaaag
      421 agtagctgaa gaaaatgggt atagcttatt attaagcggg tttgccgatc agccagaaaa
      481 aattgaacaa aaaattgaat acctgatgaa gcatgtgatc gatgggctga ttgtcttctt
      541 atccgaagaa gagtggtctg gatttgaaat gctcacaaaa atgagtattc cagttatcgc
      601 attgaattgt ccagaaggac cagcaggtat tgatacaata ttagtgaatg atcgagaaag
      661 tgtagcaaaa gtgatcgctc atcatggtga acaaggacat aaaaaaatcg gatttattgc
      721 tgccactcaa acagactacg tagcaagaga acgtttagca ggagccaaag aagcggtcca
      781 aaatgatcca tcgatccaat tagaggtctt tacaggagac tattctagaa aaagcggcta
      841 ttacggggca aaagcattat tagaaaaagg ggcaactgct atttttgtca gcaattacaa
      901 tatgtcgatt ggtgccatcg aatgtttcaa tcaagaaggt gtcagaatcg ggaaggatat
      961 tgcgttcagt cactatgatt atttagataa aaacgatctg agtatccttc cgaaaatcac
     1021 gatcacgcca ccaacagata aaatcgggga acgagcagca gaacagctgt taaaacgaat
     1081 caaaaaagaa gaggaagaaa atgaaaaagt gattgtgatg gaaaatatca tacatggaat
     1141 caactaaaac gaactaaaaa agaaaaagag gtgctttgat gaaaaaatat caatttccag
     1201 caaatttttg gtggggttct gcagcaagtg gcccacaaac agaaggaagg atttcaggcg
     1261 atggaaaagg agagaacatt tttgattatt ggtacaaaaa agaaccgcaa aagttctttg
     1321 atcaagtagg acctgaaaaa acctcgcaag tttatacgaa gtatcaagag gatgtacagt
     1381 tgatgaaaca gactgggcat aattcatttc gaacatctat ccaatggagc cgtttgatac
     1441 ctgatgaatc tggaacaatc aacgaagaag ccgttcgttt ctatcatcgt tattttgatg
     1501 agttgattga aaacggtatc gaaccctttg tgaatctgta tcatttcgat atgccgatgt
     1561 atctgcaaga aaaaggcgga tggttgaacc gagaaacggt tgaagcctat gaaaaatatg
     1621 ccaaaagttg ttttgaattg tttggttcga aagtaaaaaa atggtttact cataatgaac
     1681 caatcgttcc agtcgaagca ggttatttat atcgatggca ttatccagaa gagtcagata
     1741 tgaaaaaagc gatccaagtt ggctatcatg aagcgttggc ttcagcgtta gccatcaaat
     1801 cttaccatga aatggagcaa ggtggtgtta ttggtatcat cttgaatctg acaccaagct
     1861 atccaagaga cgaatcaaac cctgaagatg ttaaagcagc gcaaatagca gatgcgttct
     1921 tcaatcgttc attccttgat ccagctgtaa aaggcgagtt tccaagtgaa ttgattgaga
     1981 tagtaaaaga acttgatctc atgcctgtga tccatgcaga ggatttgacg gttatcaaag
     2041 aaaatacagt agatctttta ggtatcaatt attaccagcc acgccgggta aaagcaaaag
     2101 aaacaccaat cgatactaaa aacggtccaa tgcctgagga ttatttcgac aattatgaaa
     2161 tgcctggtcg taaaatgaat ccttatcgcg gatgggagat ctatgaacaa ggaatctatg
     2221 atattttgat caatgtacga gatcattatg gaaatatcga ctgctttatt tctgaaaacg
     2281 gtatgggtgt agaaaacgaa gcacgattta tcaaagaaga tggcatgatc cacgatgatt
     2341 atcggatcga atttgtccaa tctcatctga aatatgtcca tcaagccatt caagaaggtg
     2401 ccaattgttt aggctatcat atgtggacat gtatggataa ctggtcgtgg accaatgcat
     2461 ataagaatcg atatggcttt atttcagtag acttagcaca agatggaaaa agaacaatca
     2521 aaaaatcggg tcattggttc aaagacgtat cagataaaaa cggatttgag gcttaagaaa
     2581 gaatagttga gagcaaatgc gtgaaattct ccattcttac cattttgtga aaaagaagtt
     2641 caattattta cgatcttttt caactttttt gtctttctat ttcatttttt gtatataata
     2701 gaatagtcaa aattaaatag gagtggagga aagcatgagg aaaaaaagac cgtttcttct
     2761 cttttggtta ttcggttttt tcttactgct tattttatcc gcagcatttt caggaaaaat
     2821 gacagcagat cttgctcaga aagaaacaga aaaaatcgta gcaagcaaag aatcagtaga
     2881 taatttcaaa aaagaattct caacttattg gtgggcggag tataaaataa ctgccaatta
     2941 cgaaaaaggc gaattggaca tttatttgcc aaaaggaaaa gaagaaaatc aatttattaa
     3001 tatcgatgac gtacttagtg cgatttctct cagacaatat caatttaaag acaagcattt
     3061 gaccgttttg cttttgactc atgatggcga caaagtagcc cgtttgaaaa atggaaaagt
     3121 gatcaccgct tattctgaaa aagtagacaa aaaagtggtc gccgatcgtt tagcggaatg
     3181 gaaaaagaca aatgaacaag tcaagaaaac aactgtctct tctcaaaaaa acgagtcgac
     3241 aggaacaagt ccttcagatt ctggcagtgg acaaacgata acgggaagtg aagaaaatca
     3301 agcctcaatt attacgaatg agtcatcaca aggacaatat caatcctatg gacaatgaaa
     3361 aacagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac ggcaaaatag
     3421 ttcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgtgt
     3481 atcaagaggt cttgagcctg ccgtttaact ctgagccaca aggagcaatt tcaaaaaata
     3541 gctcc
//
LOCUS       DASJTM010000168         3509 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJTM010000168 DASJTM010000000
VERSION     DASJTM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.171"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..462)
                     /locus_tag="U4B29_002884"
     CDS             complement(136..462)
                     /locus_tag="U4B29_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107772.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(508..870)
                     /locus_tag="U4B29_002885"
     CDS             complement(508..870)
                     /locus_tag="U4B29_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN2107773.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(907..1121)
                     /locus_tag="U4B29_002886"
                     /pseudo
     CDS             complement(907..1121)
                     /locus_tag="U4B29_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1249..1584
                     /locus_tag="U4B29_002887"
     CDS             1249..1584
                     /locus_tag="U4B29_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107774.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1660..3210)
                     /locus_tag="U4B29_002888"
     CDS             complement(1660..3210)
                     /locus_tag="U4B29_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107775.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSFYYGKTYDIDKCNK
                     LLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRLDVFNATSVI
                     QKLNEDILPLLEKFHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEA
                     HEVLQGLSWQGTYLMTEKELTNTVCERLRNVGNNSVENALKENRGYFVKQGFTLERAS
                     KDIKRLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPLYQFTSVTRDDGRRVRK
                     RKDPIVLN"
BASE COUNT         1094 a          698 c          467 g         1250 t
ORIGIN      
        1 aaaattcccg acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag
       61 gaaactataa aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg
      121 cataatacat ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat
      181 acggtctttc tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat
      241 agctttagcc cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga
      301 tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc
      361 taatccagat gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat
      421 aactataatg ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc
      481 caaaataatt tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa
      541 gattgctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg
      601 atataagtag cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc
      781 agttgtctca tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt
      841 tatttctttg ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt
      901 tattagttaa ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact
      961 taatatctca gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct
     1081 ttttcttttc tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt
     1141 attatataca acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca
     1201 ttacaaatac aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa
     1261 caatatgcaa aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta
     1321 gctgaaaaaa tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag
     1381 cctaacctcg atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt
     1441 ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc
     1501 actgaagatg cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa
     1561 cgcaatcagc tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt
     1621 attgagatga aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc
     1681 ttttctcttc cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg
     1741 aactaattca actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt
     1801 tgctaggtag actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt
     1861 agaagctcgt tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc
     1921 attttccacg ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc
     1981 tttctctgtc attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc
     2041 atctaaaatc aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct
     2101 tttagcctct ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt
     2161 aaaatgaaat ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt
     2221 tgcattaaaa acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc
     2281 tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc
     2341 taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat
     2401 gtcataagtt ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat
     2461 acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata
     2521 gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga
     2581 ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata
     2641 caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat
     2701 tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa
     2761 aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa
     2821 cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct
     2881 ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg
     2941 tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag
     3001 ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat
     3061 cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt
     3121 atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt
     3181 ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa
     3241 tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc
     3301 aaacgcagaa ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact
     3361 atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc
     3421 cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt
     3481 gcttggaata aatgattgtg atgaattta
//
LOCUS       DASJTM010000169         3491 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJTM010000169 DASJTM010000000
VERSION     DASJTM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.172"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2241)
                     /locus_tag="U4B29_002889"
     CDS             complement(<1..2241)
                     /locus_tag="U4B29_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107776.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTT"
     gene            complement(2913..3181)
                     /locus_tag="U4B29_002890"
                     /pseudo
     CDS             complement(2913..3181)
                     /locus_tag="U4B29_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          981 a          770 c          551 g         1189 t
ORIGIN      
        1 tgtagttaaa ccggtttgta gcgtttctcc ttctcggttc tgtaactcaa aaacagctcc
       61 ttgaaggact tcaccactgc cttcagcatg tttaattagt tcaacaccac ccgtatcaag
      121 gatgttttca atggttaacg tcacttcttc tatggagtat tcatttataa aaacatcatg
      181 taccgtcgca tctaaaagat acccttccgg tgcttctgtt tctcggactt gatagctacc
      241 tacaggaagc ggttcactta aaaattctcc gttttctgcc gttatacctt cttgtatcac
      301 ttgtgaatcg cttgataaaa tttcaaattt tgctcctgcc agcctttggc cattctcatc
      361 agttttaatt actttaatac gcccatcgat tttccttgtt cttacagtac tcgtattcaa
      421 tcgattgtca ctattcacaa gcgttgcaaa gtttaaaatt tgatcgccga ccgcatacgc
      481 ctctacattg accttaaaag agagggtaaa acttgtcgga ccaccaatcg tattattcgc
      541 actgaaacga actattccat tattgacttc ggcatcaatg aaagagggaa tatctgatag
      601 aaatgacaca aattccatgc cttcttccaa aacatcttca ataacaagcg tatttcttgt
      661 tccagaaaac atcgagacat gcagagtata cgttagttca ttttcattga gattgataac
      721 atttttgtcc acactttttc ttaaagagat tgccggaaga ttactttcag ggattctcaa
      781 gcggtagtct cggctcatta cctgattatt gtaggtcata ctcgctcgat tccgagaaaa
      841 cccatcctct tgtcttcctt cattgaacaa gtcgacatcg gtcacttcaa gagcatattc
      901 aataaaatac gattgaccct ctgctagatt accaaagtcc acaacaagtg tattcgtttc
      961 cttattaaaa gatactttgt ccacaaattc ctcggaaaca taatgagttc taacgtttgc
     1021 ttcacctgga ttcgttactt ctgcgatccg aaccccacct gaaataggtt ctctatttcc
     1081 agtggaactc catgtagtcg gattcggtgc caaagttgtt ccttgcggta gtacatcggt
     1141 aaccactaca ccgtcttttc gtgatccttc ataatttacc tgcaaaataa atttattact
     1201 gttggtcacg tcttgcccgt caatcagcag attcccaaga tttccttggc tgtcgatttc
     1261 tctagagcca tagtataatt tcgaaaaaat gggtcttgat gaaggaatag gcgtaattga
     1321 aaattccgtg tttgctgctc gctcagcata agtaagactc acttgaaagt cattatttgg
     1381 ctcagtaccg cctgattgaa agaaagcaac ttcaaacgtg atactgaatg tatacgactg
     1441 agcggcttca tttaatcctt cataatcaaa ggtaaatgga aaaacaacgt ctgtgccttc
     1501 ttggtaaaat gccagtggct ttaaaggtga atcatcagcg acctccactc taatgaactc
     1561 aattaagcca atcgatgctc taggaactcg tacgaccatc tccccatatt cttcaagaat
     1621 atcgccagcg cctccttgaa tgtatccctt taacgtttgt gtatgcctgc tcggcaaagt
     1681 ggggcgctca gcagagatgc cgatgtcata attcaagagc tcctcttgtc tttcaatctg
     1741 agattcctgc gttatagatg gctcagatga tctggcttca ttaaaagatt cacttaagct
     1801 ttcttctggt atattaaaag attcacttgt gctttcttct ggtacattaa aagactcact
     1861 tgtgctttct tctggtacac tgatttctat tgagaccgta tttgattgaa tatcaccgct
     1921 tcttgcggtc aattcgtatt tacctgaatg ctgggaagaa taagtaatca gacgttcgta
     1981 atcattgagt tgcttcactt ccccttcaat caaatcctca ctcttatcaa tgaccaatgc
     2041 atttagcagg tctggtgttc ctctgactcg caactggaac tgctcaccca aagcagcctc
     2101 ctcttgatca acttcgatcg tcagcgaacg ctctggctgt tctgctaaag actcagctag
     2161 aaccgttatg ttcccaaatt gattgatcac aataagcata actagaaaca tacttaacag
     2221 tttatgaaat ttgttcctca ctattactcc cccttataat tttgactaaa caacaaggta
     2281 cccgataatt ttctaaaaag attccccaac tcaaaatggc tgcgttacct ccctcgtatt
     2341 ttcggttcaa ctcaagcgtt gcaatgtaag ctgtaaacaa agctaatgat tgccgattgt
     2401 cttctgttga ttgattcgat agctttgtat gacttttagt tgtccgtgtg gcttaaccat
     2461 aattatgatt tcacgattcc tcctgccagt gccttgcaca agctggagcg taaccctaat
     2521 agttattttg ttaatgtact tatatacttt tatacttttc cattagaaac aatcactaaa
     2581 tcgttgatac caaggtcaga ctctagaatt tcatcagtga gtccctgtat tggttaacct
     2641 tcatcaatcc caaaaaaata tatcaatgct tagcatcaaa atgaatatat agtttagaat
     2701 acttgggatt catcgataac tgatcgctta tttggccata ttatttggca cttggttcaa
     2761 ctaaacgcat ttaggatgtt ccttcattct cgtcatggat gttctcaaac cgtcattact
     2821 aataaactgt ccaccgaatc gatggttcat ttcttgcatt ttccacgtcc aattactctt
     2881 tttgctcttt atttgggcgt agtttcactt tcttaacttg tatcgtcttc catcaactcc
     2941 ttaatcatct tcttagcttt attggctcgg tttccttgta aaaggtatct acaaatagtg
     3001 acaatctgta ttaaatcttc atcattttct gtaatgtcaa taatttctat attctgtacc
     3061 atatattgca aagggtttca ctaagttcat aaccgaagcg aattaaacaa cttttgtata
     3121 gaataactac cttttctatt tttccattgg taattctatc tattaattgg tttaatcctt
     3181 ttgttgtagt tgattccact accaagatga ttctctcgaa ttttaaagct tgaagatgta
     3241 ttgctatttt tgctaactgg cgtatttgca actatttttc ccttctattc ttccttcatt
     3301 gattcccctc taaaattact atccataaaa aattgcataa atgacaatag tatttatatt
     3361 agtcatactg ctttttaaca cagcaaattc cagaaaaaca ttcgttacat atttgtgaat
     3421 ttttccaaaa aataacattt ttataaaacg acgaaaaata aatcactacc agtcaacttc
     3481 tataatatta c
//
LOCUS       DASJTM010000170         3416 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJTM010000170 DASJTM010000000
VERSION     DASJTM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.173"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(554..2167)
                     /locus_tag="U4B29_002891"
     CDS             complement(554..2167)
                     /locus_tag="U4B29_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2107777.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="U4B29_002892"
     CDS             complement(2308..3216)
                     /locus_tag="U4B29_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107778.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1127 a          572 c          595 g         1122 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaa
//
LOCUS       DASJTM010000171         3382 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJTM010000171 DASJTM010000000
VERSION     DASJTM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.174"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..523
                     /locus_tag="U4B29_002893"
     CDS             260..523
                     /locus_tag="U4B29_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN2107779.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            517..867
                     /locus_tag="U4B29_002894"
     CDS             517..867
                     /locus_tag="U4B29_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2107780.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(891..1505)
                     /locus_tag="U4B29_002895"
     CDS             complement(891..1505)
                     /locus_tag="U4B29_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107781.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1819..2241
                     /locus_tag="U4B29_002896"
     CDS             1819..2241
                     /locus_tag="U4B29_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107782.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2251..2454
                     /locus_tag="U4B29_002897"
     CDS             2251..2454
                     /locus_tag="U4B29_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107783.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2665..3249
                     /locus_tag="U4B29_002898"
     CDS             2665..3249
                     /locus_tag="U4B29_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107784.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1167 a          491 c          602 g         1122 t
ORIGIN      
        1 tccattttgt atcaataact atgaaatctt taaagtatcc agaagagatt atttttagat
       61 caattttttt cttacctgat tctaaagatt ttgttttctt aaacatatta tgatttcctt
      121 tcgactattt tcatataaat gttaacatag tttatggatt catggaatgt taatctaatt
      181 taatagaaaa ggataattaa gtatatacgt tttttcttat gtatgttata ctatgtatat
      241 acagaaagag gtgtctacta tggaaatgtt acctataaaa aaatggggaa atagtaatgg
      301 ccttagattg ccaaagtata ttatggaata tcttggaatt catacagaag acaaagtgaa
      361 gataatacaa gaagaagtaa acggtcagaa gcggctaatt atcgaagcgg ctaatataga
      421 aaatgaatta acaattgaac agttatttga aaattacact gatgaaagaa atcatgtgac
      481 gattcaaaat ttaggtgaag cagtaggtaa tgaaaaatgg tgagaaaacc tagacaaggc
      541 gatattctat tattgaatac agcacctcga tctggacacg aacagacagg taaaagacct
      601 tatattgttt taagtcatga tattattgcg gattatagta acgttgttac agttgctcca
      661 atttctagta caactagaaa ctatccgcta tatgtaaata taaactcaga atatgaaatg
      721 aagacaacag gaaaagtatt attagatcaa ctaacgacaa ttgattatga agccagagag
      781 tgtatttttt tggaaaaagc gcatgatagt ttagttgagg agttattgat gaaagttaga
      841 acagtttttc aaaaagtaag caaataaaat agaaacagca gcatgatcaa ctattcgtga
      901 ttgaccatgc tgtctttttt tattctatat acggtttgtc tcgtaacatt gtagtctttt
      961 gcaatcttag cgatcgcaac accttttttt agatcctcaa cgatgttttt ataaacaaga
     1021 cgacgttggg gatcttttgc attggcactg tatagcttag gacgtccttt ataaacaccg
     1081 ttcgcttttg caatcttaat gccttgtgcc tgacgtcgtt tagattcggt acgttcttgt
     1141 tcagcgatca tggccaaaat ttgaatgatc aaatctttaa tgaatttatc caggagagga
     1201 ttgccaattg cttcggccat gatcggtaga cttgtgatga tcagctgcac atttttcttc
     1261 ttcaagtaat tgactgaagc gatgatctcg tcgtaatttc ttccaagtcg atcaatagct
     1321 tccacaataa aaatatcttg atctcggacg aaatccagtg cttcttgaaa aacaagtcga
     1381 tgagtaatcg ttgcgccaga ttgtttctca atgaaaattc tctctgcacc gaattttttc
     1441 atttcttcaa tctgccgttg ttcattttga tcgatggatg agacacgtat gtatgcaact
     1501 ctcaaaattt ttacctactt tctgcaacta tttaaaaaac accctaatca tacacgagaa
     1561 aaccagattt ttcaatgtcg cggtcgtgtc tagttagggt ataccttata tagacaaaaa
     1621 ctaatgatgg aggaaatagt atgtgtcact tttcttttta aatggcaata ttttttgtaa
     1681 ataaatgtca atcaagaaaa tttaaacgat tatgacatta aaaatatatt ttatgaattt
     1741 tctagtatat aacttgattt atctgttaag atttattaag aaaacgtttt cttatttttg
     1801 atttcgaggt gatagacaat gaaaaataag tttattttat ctttaggtat tttaactatt
     1861 ggaattagtg taactggaac tacaattcct acatttgctg atagtacaaa tgaaccaact
     1921 actgtagtag ataataacca agaattaact cctttagata ggaacggaat agccattggt
     1981 aacccaacta acgacttaac tgttactgaa aattatttaa tagtttctcc attacttgct
     2041 cgagcagctt ctaaaacgat acgagtgaca ggaacgatga tttttgataa agttggttgg
     2101 ccttctagac ctcctcaatc acggaattgg aaagaaaaaa gaaatggtta ttggtactat
     2161 ggaaaattgt atttacaaag ttataaatca gctggaaaca attggattgc gacttattca
     2221 ggaaccttaa ctagaaagta gggatttttt atgccagaat ttgtgaacac accaaatttt
     2281 tatcaagata gcgatattct aagtaaaatt aatttaccga aagaagaatc agctttacaa
     2341 gttcagcgaa ctttacagga gacacaaaaa attcaaaaat caatttctaa tctagaagat
     2401 tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc aaaaagaaaa ataatgattt
     2461 taaaaaaaga gtgtctagtc gacattcttt ttttctaatt gaaagtgttc ataaaatcac
     2521 ccatttctgg acactttctt tttttgcttt atttttcaaa aaaacaattc atttcatgtt
     2581 cataaaccca taacaatatc tatgttcagt taaactatat ggataaagtt atgttacaat
     2641 aaaattaatg aaggagtgat ttttttgaaa ataggatacg cccgggtatc tactggttta
     2701 caaaatttgg atttacaaga agacagccta aataaatttg gttgtgaaaa aatttttact
     2761 gatcatatga gtggggcaaa aagtaatcga ccaggattag aaatggccat tgattttgtt
     2821 cgctctggcg atacacttgt cgtttggcgc ttagatcgtt taggtagaaa tatggaagat
     2881 ttgatttcaa tcgtcaatcg actgaatgag cgtggtgtga gttttcatag tattcaagaa
     2941 aatattacga tggacaaatc aagttctaca ggacagctca tgtttcattt gtttgcggca
     3001 tttgcagaat ttgagcgtaa tttgatcctg gaacgatccg cagcaggacg agcagctgct
     3061 cgtgcaagag gacggtttgg tggtcgtcct gaaaaattgt ctgaacaaga tttggaatta
     3121 ctgaagactt tagtggatag cggtacacct attaagacta ttgcggaacg gtggaacgtc
     3181 tcaagaacca ccatttatcg ttatttggca aaaatggagg agaaaaatgg aaagacaaat
     3241 tctcattagt ttttgtcatt taatgctgaa aatttttgcc atttatctag aaaagtgaca
     3301 atagtatgag aattttttgt aagtgtctac tgataactta ttctcaaatg ttgtaaggat
     3361 tttattcatt tgttaatatt aa
//
LOCUS       DASJTM010000172         3368 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJTM010000172 DASJTM010000000
VERSION     DASJTM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.175"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..1158
                     /locus_tag="U4B29_002899"
     CDS             118..1158
                     /locus_tag="U4B29_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2107785.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1982..2314
                     /locus_tag="U4B29_002900"
     CDS             1982..2314
                     /locus_tag="U4B29_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN2107786.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2933..3274
                     /locus_tag="U4B29_002901"
     CDS             2933..3274
                     /locus_tag="U4B29_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN2107787.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1255 a          457 c          640 g         1016 t
ORIGIN      
        1 tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt tttatttatc tttgctattt
       61 tcgaaaagag taggtaattt tacataaata aaacaacgtg aaagtagagg atctacaatg
      121 actgatttta aattttttaa ggcggataga gtttacaacg aattatttta tcaatttcca
      181 aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag attcaactaa gattgcctat
      241 atgcttttaa aagcaagatt agagatcgca atcagcaaac ggcaaatcga tgaagaaggt
      301 aatgtttatt ttacttatac gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa
      361 aaagcgatag caatcaaaaa agagttggaa tcctttggtt tattattaca aaagcagatg
      421 ggatttaaca aacagttagg gaaaaataat cctaatagac tatatctagc agaattaaaa
      481 gtctcagaaa atgatatcta cttactcgaa aaatttgata gagagaatag ggaaaacgtt
      541 gataaatcag agggtatgaa aatcataccc accctcgacg aaaaatcaga cgctgaatcc
      601 cttggggctc aagagggtat gaaaatcata ccgtgccaaa acgttgataa atcagagggt
      661 atgaaaatca taccagaact taataataat atattagaca ctaatagaca caatatagac
      721 actgaaaaag accgcctaca agatcaattg ttgttagaca attttgagac aattatgaca
      781 aacgacagca ttgctacgtt tgtccctgaa cgagtattaa atttgataaa aacattttct
      841 tcaagttaca gtgaagctca aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct
      901 gaaatagaaa gtgatatttc gatagttttt gaagaactcg atagttatta tgtcaatgca
      961 gaacaagaat tatacacgac actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc
     1021 gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata
     1081 gctgctaaag tatcaagtga aaaacgtttg aattatgcaa gctccccaag cactattacg
     1141 aaagactggt tagagtgaaa ctttactgac aaagtgattg ttgcttcatg gagtaacatt
     1201 aaaattaaaa attttttatt attctaatta tattacgaat attacaaaat aatagctatt
     1261 ttaatagaat ttataagtta aaatattatg ataaaaatca caaacgtagc cttttacgtc
     1321 ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat gtttgcatac
     1381 tccttttttt tgagttataa taatacgcga agagagataa tttcattatt taaacaagct
     1441 aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta cattttttgt
     1501 ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca cttttggatg
     1561 ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg
     1621 taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg ttaacctttc
     1681 agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag tgataccact
     1741 ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat gcctaagcat
     1801 aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa attagaggga
     1861 acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc aattctctat
     1921 ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg tggtttattt
     1981 tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta aacggtgtac
     2041 cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg ttgtgcagtg
     2101 taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt acattgggaa
     2161 acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc caacaatcaa
     2221 acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg
     2281 taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata acttgtattt
     2341 tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt agagtcacta
     2401 gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga cggggtaggt
     2461 tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg
     2521 gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt tagaagttag
     2581 acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct agactttgac
     2641 gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt
     2701 gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga tcaatcgagc
     2761 atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt
     2821 atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa
     2881 aattaatata aagcatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa
     2941 ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga
     3001 ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc
     3061 attagacgag gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt
     3121 gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg
     3181 agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg tcagctttga
     3241 aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat
     3301 aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt
     3361 aaggaatt
//
LOCUS       DASJTM010000173         3324 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJTM010000173 DASJTM010000000
VERSION     DASJTM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.176"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..924
                     /locus_tag="U4B29_002902"
     CDS             <1..924
                     /locus_tag="U4B29_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2107788.1"
                     /translation="FGSKKNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEK
                     IKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFD
                     IIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEA
                     KSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNA
                     ENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKI
                     LFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            940..1980
                     /locus_tag="U4B29_002903"
     CDS             940..1980
                     /locus_tag="U4B29_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2107789.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1999..3084
                     /locus_tag="U4B29_002904"
     CDS             1999..3084
                     /locus_tag="U4B29_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN2107790.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3117..>3324
                     /locus_tag="U4B29_002905"
     CDS             3117..>3324
                     /locus_tag="U4B29_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2107791.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFS"
BASE COUNT         1229 a          467 c          609 g         1019 t
ORIGIN      
        1 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
       61 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
      121 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
      181 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
      241 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
      301 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
      361 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
      421 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
      481 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
      541 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
      601 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
      661 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
      721 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
      781 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
      841 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
      901 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
      961 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
     1021 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
     1081 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
     1141 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
     1201 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
     1261 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
     1321 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
     1381 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
     1441 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
     1501 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
     1561 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
     1621 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
     1681 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
     1741 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
     1801 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
     1861 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
     1921 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
     1981 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
     2041 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
     2101 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
     2161 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
     2221 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
     2281 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
     2341 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
     2401 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
     2461 gaaagtctgt ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt
     2521 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
     2581 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
     2641 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
     2701 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
     2761 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
     2821 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
     2881 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
     2941 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
     3001 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
     3061 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
     3121 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
     3181 gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat
     3241 ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta
     3301 aaatttattg gggtggtttt tcca
//
LOCUS       DASJTM010000174         3304 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJTM010000174 DASJTM010000000
VERSION     DASJTM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.177"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..463)
                     /locus_tag="U4B29_002906"
     CDS             complement(<1..463)
                     /locus_tag="U4B29_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107792.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQL"
     gene            complement(479..844)
                     /locus_tag="U4B29_002907"
     CDS             complement(479..844)
                     /locus_tag="U4B29_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107793.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            complement(850..1593)
                     /locus_tag="U4B29_002908"
     CDS             complement(850..1593)
                     /locus_tag="U4B29_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107794.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            complement(1724..2446)
                     /locus_tag="U4B29_002909"
     CDS             complement(1724..2446)
                     /locus_tag="U4B29_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107795.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(2452..2697)
                     /locus_tag="U4B29_002910"
     CDS             complement(2452..2697)
                     /locus_tag="U4B29_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107796.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            complement(2708..3292)
                     /locus_tag="U4B29_002911"
     CDS             complement(2708..3292)
                     /locus_tag="U4B29_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107797.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
BASE COUNT          952 a          608 c          437 g         1307 t
ORIGIN      
        1 acaactgttc atgatctggt ctaagcgtta gaatatctaa attatttatc ttttttaatt
       61 gtgtaataaa atctattttg gattttcttt caatatcaaa accgacaata atctttcctg
      121 ttggatcaac gcatttcact tgaataactt tgtcattttg cgatagctct aatattgatg
      181 atttttcttt tgcctcttcc aaaagatgtt ctaacacttc gttaggtgtt ccatgatagt
      241 aataaatcat ggtcttctcg aaaattttta tcggatcaac accatattct tttccgattc
      301 gataaaaaaa tttcattgga atttcttccg gatttctgtt cctgcttgac cagttagaga
      361 tggtttgata atttgcaaaa tggattcgtt ccgctaatct ctgaactgtg gtctctaaat
      421 cttcttctat taattgtttt acagcactaa tgttttgtga catttgcttt ctatcccttc
      481 aaattcgttg cattatcaat tctaagattt catgttttag tgcatttcta tctacatttt
      541 gcttttttaa ctctgcacca taatatcttc gcaatccctt atccaagcat acaattcctt
      601 gatctaatag atcatattcc gctttttcaa accttcgctt tgcctgctca acaagcaaaa
      661 tgatattgtg atgtatctga ttaaactgcc gatcatattc ctctaaagtt ttagcatcta
      721 agttagcaat atgatcctct gctaattttt gtgttaaaaa taattcgact ttctcagatt
      781 tcaacatctg tattttttca tactcttgtt ttgcttggat atagtcatct ttcaagctag
      841 acatctaact catctccttg cctgtataaa ctgatacgga ctgtccaaaa ttgaataggt
      901 tggtgatgac ttcaagaaga cttcaccttt ttcacatgaa aataaaccaa ttaattcttc
      961 aatttcattt acgatcagat atttattagt taaaattttc ccattttctt tttctagctc
     1021 gacaatcgct tgattccatg tattttgcac taaatcttga atcaatttca atctatccac
     1081 ttttttcatc tcatacaggg caatttcagc ttgtttaatg ccttttaatt tgcgaaaggc
     1141 atatttgtat tgtgtgtagg ttaaaaagcc tgtgtagtta tacatgggct ttcctgatgt
     1201 cttgtagtca tttaagcttg gatatatttc agttttccat ttttcttctg aacgttgatc
     1261 aataattctt aatttttcag gtaaatcaat ttcatagtat tcttcaattt tcagctcacg
     1321 ataaagtaga tgtttttcct tttctttttc aattacaaaa gaattatttc ccttcagttt
     1381 agctatttct tcacttgtga gaccaagcct aattttttct acttcacttc taagttcacg
     1441 aatacttgtt gcctttaacc acttcccatt tgcaccatta ttgtaaccgg taagtaactg
     1501 tattttataa attccttcac catgaagcac cttatatata ccaacctgat tcttttgcgg
     1561 aatatgagct tcatttgcac tgatttcttt cattacgcat tctcctttat aatctagtta
     1621 attagcttgt gtgtttcgag tagttgagta agaacgtttg tttcagaaat catttatttg
     1681 ttatcactta attttaaaaa aatagggctt acgccctaaa gatctattta actttggatt
     1741 gatcgtccat aacaggctct gattgttgat ataatttata tgctcccaaa tccatataat
     1801 atttagttgt actttcaatc atttcatcag ttgtatttgt tgtcattccc attacatccc
     1861 agtatagtgt gccgttaact ttctttggtg ttactactgc aactgttggc gtatgatatt
     1921 gattgttctc atcagccaga ccaggaatgt tttgtaccca ctcaggtaca ccatcagtaa
     1981 tatctacagc aactgcgtaa tactcgtaag ctggtttatt tatggtaata tcatgcatta
     2041 atgttggagc ataactaaat ccttctttta tttcttgcat gatttttgat tctgcttttt
     2101 gacaatctgg acaatcaggt ttgtaaagaa cgatagtcag atttttattt tgcaatggat
     2161 cattcccata attttcatca ttttttactg tattaataaa gttgttataa acttcatccg
     2221 gcttttgacc tttattaatg tcttcgtatt tttgatactt ttcagcatta ttttcagtca
     2281 ttgaagacat tgttgttgat gccgatgtag tgctgttttt agtaaccatc gtactactag
     2341 actttgtgtc tttgttatta ttagcactat tagttgtacc acatgctgta agggttatta
     2401 atgctgctgt cgataacaat acaaccttca atttagttga tttcatttta ttcacctatc
     2461 cttttttctt ttgtttaaca ataaaagaat aacccgtact atccaataaa acaacagcat
     2521 caatatggaa acccaaacca tttgtacaat ccaagcataa agactaaata ataggtattg
     2581 tggatttttt tgaatgattg tatttgagat gccatttcct acagtaaaat acattgttac
     2641 gaaaataaca ccttcaatta tcaagtgatt tgttggttga tttataattt ttttcatatt
     2701 tttactccta aactaaattt attcttcctg ctaaatattg aaaattatat gggttctgca
     2761 catattccct aagttcctta atacttgttt caatatcacc aaaggctgtt ttaatgtttg
     2821 ctgacccatc tttatataca atcatttccc cttcacccat acaaaaagaa atttcagtat
     2881 tagaattgat tgttttttca gcacgaccat tgataatttc ttgaccatca cgccatataa
     2941 aatacataag ataaaatacc tcataagcgt tgtatccagt atccgttgtc attgatacca
     3001 aaacatcagc atcttgtatt cctgataaag ctaatccaat ttcatggatc acatcaactt
     3061 cattttcttc ccaatctcca ctttcgaaga cggtataggt ttgattgtta gcaaatggtt
     3121 ttctttctat atcgaacatt cctttccaat caagaatcat gtacccgatt aaattgtgaa
     3181 agaaaattag ataaaataaa tacaataaga tgctaactac aaaaatatca gccattattg
     3241 acataggatt atcaggatat aaccagatgt aaaacgattg tttcaaatcc attttttcat
     3301 catc
//
LOCUS       DASJTM010000175         3201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJTM010000175 DASJTM010000000
VERSION     DASJTM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.178"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4B29_002912"
     CDS             301..585
                     /locus_tag="U4B29_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107798.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4B29_002913"
     CDS             588..1004
                     /locus_tag="U4B29_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107799.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4B29_002914"
     CDS             complement(1141..1860)
                     /locus_tag="U4B29_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2107800.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2514..2609
                     /locus_tag="U4B29_002915"
     CDS             2514..2609
                     /locus_tag="U4B29_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2107801.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..3157)
                     /locus_tag="U4B29_002916"
     CDS             complement(2912..3157)
                     /locus_tag="U4B29_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107802.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DASJTM010000176         3196 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJTM010000176 DASJTM010000000
VERSION     DASJTM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.179"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..147
                     /locus_tag="U4B29_002917"
     CDS             <1..147
                     /locus_tag="U4B29_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2107803.1"
                     /translation="QTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIK
                     ISNE"
     gene            162..1031
                     /locus_tag="U4B29_002918"
     CDS             162..1031
                     /locus_tag="U4B29_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN2107804.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1012..1746
                     /locus_tag="U4B29_002919"
     CDS             1012..1746
                     /locus_tag="U4B29_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2107805.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1779..2687
                     /locus_tag="U4B29_002920"
     CDS             1779..2687
                     /locus_tag="U4B29_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN2107806.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2684..3164
                     /locus_tag="U4B29_002921"
                     /pseudo
     CDS             2684..3164
                     /locus_tag="U4B29_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1118 a          454 c          702 g          922 t
ORIGIN      
        1 cagactccac agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg
       61 aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga
      121 gaagaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc
      181 aaatagtaac agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct
      241 cacgtgcaag aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt
      301 cagactcatt tgacctgaca gcgattaatc aaattgctac agaattggat gataaaaata
      361 gaaacaacct tgttgtgcct cccggagcgt ggggtgattg gattaatggc ggcggatggt
      421 tatttataaa tgggtatcat gttgacttga ttttacgtga tataaaacgg gtagaacaaa
      481 taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg
      541 gttatataag tgcaatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga
      601 acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa
      661 gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg
      721 cgggggtaga ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa
      781 atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac
      841 tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg
      901 aagtactcgg tctttctctt tttgaatgct acgatatgac cgagaaactt tataaagaag
      961 tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa
     1021 aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa
     1081 ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt
     1141 aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac
     1201 ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga
     1261 aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca
     1321 gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag
     1381 aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt
     1441 gaacatcctg tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa
     1501 atactgcatt tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg
     1561 gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca
     1621 aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt
     1681 ccggggatgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag
     1741 atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga
     1801 tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg
     1861 ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat attacatatt
     1921 ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata
     1981 taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt
     2041 cctatcttat gctatttgat gattacaata aaattgatct taccttattg cccttggaag
     2101 agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta
     2161 gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa
     2221 gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat
     2281 tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc
     2341 tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca
     2401 agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca
     2461 cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt
     2521 tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata
     2581 ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc
     2641 tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa
     2701 agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat
     2761 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     2821 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     2881 tattgacaat actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg
     2941 atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc
     3001 ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat
     3061 tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
     3121 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     3181 aattggagtt cgtctt
//
LOCUS       DASJTM010000177         3166 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJTM010000177 DASJTM010000000
VERSION     DASJTM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.180"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..775
                     /locus_tag="U4B29_002922"
     CDS             179..775
                     /locus_tag="U4B29_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2107807.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="U4B29_002923"
     CDS             899..2698
                     /locus_tag="U4B29_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2107808.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..>3166)
                     /locus_tag="U4B29_002924"
     CDS             complement(2976..>3166)
                     /locus_tag="U4B29_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107809.1"
                     /translation="SYICKPIGVSHELIGCIERTYINTAVVYVESYDQRDYQLINESK
                     YRMLVKFCDISAPAELVS"
BASE COUNT          901 a          560 c          588 g         1117 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gagatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatc
//
LOCUS       DASJTM010000178         3162 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJTM010000178 DASJTM010000000
VERSION     DASJTM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.181"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..153)
                     /locus_tag="U4B29_002925"
     CDS             complement(<1..153)
                     /locus_tag="U4B29_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2107810.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(228..523)
                     /locus_tag="U4B29_002926"
                     /pseudo
     CDS             complement(228..523)
                     /locus_tag="U4B29_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(783..1331)
                     /locus_tag="U4B29_002927"
     CDS             complement(783..1331)
                     /locus_tag="U4B29_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107811.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1332..2189)
                     /locus_tag="U4B29_002928"
     CDS             complement(1332..2189)
                     /locus_tag="U4B29_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2107812.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2475..2761)
                     /locus_tag="U4B29_002929"
                     /pseudo
     CDS             complement(2475..2761)
                     /locus_tag="U4B29_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2751..3080)
                     /locus_tag="U4B29_002930"
     CDS             complement(2751..3080)
                     /locus_tag="U4B29_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2107813.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          934 a          637 c          403 g         1188 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaacccttg atctcgctta
      241 ttacaatcgc tttcatttgt cctaagagtc aaatcttttt ccaccataac ttttacaccc
      301 aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt taaactgaca
      361 attagctcat tgggtacata cttatttgag agtcatctaa ttggctcata atcctttctt
      421 tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac atactcaccc
      481 caacaaccaa tcctaaatat attttctttt taaacttttt cacctacatc tcctcctaat
      541 agttttattt tagagttagc tctattactt atttactaac tcaacaacag attatcatac
      601 acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata accgttccta
      661 tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct ctatttcatg
      721 caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt cttctttgat
      781 tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc ggtatcttgt
      841 tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg aacaattgga
      901 tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc ttgcttatct
      961 tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa ttcatctctt
     1021 gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa caaagtcgac
     1081 agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg tgcgccaact
     1141 ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat ttcttgaact
     1201 tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt tttctttgct
     1261 tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg
     1321 atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa ttctttcaaa
     1381 atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc tttctttgtc
     1441 ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt gagtacaaca
     1501 tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc ctgatctact
     1561 gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt tgtatcaaat
     1621 tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat cgattttact
     1681 tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt actatataca
     1741 gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa tggttctagc
     1801 ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt gatccaatca
     1861 tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat tactttatag
     1921 acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt ttcgtaaaca
     1981 atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt taagttcatc
     2041 acatcagaag tatttccttg catatccaag tcaattatca aggttctttt tccttcatta
     2101 actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc acctttaaaa
     2161 ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta gcaatattca
     2221 tttgatacta ttataacata tttattttta gttactacca aaaaaaagat atatatgtac
     2281 taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga aatatattga
     2341 tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag agaatactcg
     2401 cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc cttcttaaga
     2461 acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc atgcgttcca
     2521 cataacaata ctggaataac cttgttttct ataatctcgt aagctactct atatgttgtg
     2581 cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt ttgttcaccc
     2641 ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc tttcaatggc
     2701 ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt tcataagcca
     2761 atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt atcttcagca
     2821 atatctctaa aggaagaatg cattttgttt tttctattct taaattctac aagaatctca
     2881 tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa atcataaaat
     2941 tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt tgcttcaact
     3001 tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga attgccttgt
     3061 tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat tacttagtaa
     3121 atacattata ccacgagata atgtatttac aagtaattac tg
//
LOCUS       DASJTM010000179         3106 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJTM010000179 DASJTM010000000
VERSION     DASJTM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.182"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1002
                     /locus_tag="U4B29_002931"
     CDS             <1..1002
                     /locus_tag="U4B29_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2107814.1"
                     /translation="KGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQ
                     QELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMG
                     TQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSC
                     VRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIER
                     SYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1014..2018
                     /gene="neuB"
                     /locus_tag="U4B29_002932"
     CDS             1014..2018
                     /gene="neuB"
                     /locus_tag="U4B29_002932"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN2107815.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2015..>3106
                     /gene="neuC"
                     /locus_tag="U4B29_002933"
     CDS             2015..>3106
                     /gene="neuC"
                     /locus_tag="U4B29_002933"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN2107816.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLN"
BASE COUNT         1176 a          404 c          591 g          935 t
ORIGIN      
        1 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
       61 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      121 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      181 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      241 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      301 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      361 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      421 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      481 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      541 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      601 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
      661 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
      721 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
      781 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
      841 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
      901 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
      961 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     1021 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     1081 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     1141 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     1201 aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta
     1261 ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg
     1321 aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag
     1381 atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat
     1441 ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc
     1501 cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat
     1561 tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat
     1621 tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt
     1681 ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat
     1741 ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc
     1801 gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg
     1861 agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa
     1921 atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa
     1981 gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg
     2041 aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac aagataattg
     2101 ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga
     2161 attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa
     2221 caataaggga aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt
     2281 tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc
     2341 tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc
     2401 tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc
     2461 tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt
     2521 tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta gttttagaga
     2581 tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc aagtatttca
     2641 tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga tcaattcaat
     2701 ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat
     2761 tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta cgaatttaga
     2821 gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc
     2881 tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa
     2941 tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac
     3001 tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat
     3061 ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaat
//
LOCUS       DASJTM010000180         3017 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJTM010000180 DASJTM010000000
VERSION     DASJTM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.183"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(379..624)
                     /locus_tag="U4B29_002934"
     CDS             complement(379..624)
                     /locus_tag="U4B29_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107817.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(618..1019)
                     /locus_tag="U4B29_002935"
     CDS             complement(618..1019)
                     /locus_tag="U4B29_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2107818.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1206..3014)
                     /gene="uidA"
                     /locus_tag="U4B29_002936"
     CDS             complement(1206..3014)
                     /gene="uidA"
                     /locus_tag="U4B29_002936"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN2107819.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          942 a          591 c          382 g         1102 t
ORIGIN      
        1 aatcttttcg agtagctgtt ttcattatag caccttcctt actttttttc atttatttct
       61 atcagctgct tttgtcaaaa gcacatagga atttcacctc tccccaagtt tacggcgagc
      121 cttacggaag tatcattatc ccaactttaa atcatcgctt tttattgact ataaaaatta
      181 ttttagaata tttatcgctc cttctttaca gcaagaaatc atggcgcaac taaaatatgc
      241 tccttaaagt gttaacgtac tgatagaatc tatttaattt tcaatgaaca ataggttttc
      301 tacctaaaag aatctactaa ttccttaact attagaagat tcttttaaac agaaaaagtg
      361 gaaaggggat ttttttaatt agttaatttt aaattttaag atcgcttcta ttaatttaat
      421 ttctagctga cttatcatat attcatctac ataggtaacg aattgaccta cctcgttaaa
      481 tccatttgtc aaacataact tagccatata agaatgataa tgtttgagta tcatcttgat
      541 cgaaattgga tctccttcta gagcagataa gataatggat agtggaacta ataggtactg
      601 ccgcttttta gatttatcta ccataggtca taccccattt tcaattgttc aactgccttc
      661 tttcgttgat accagatacc acttgtgctc atattgagaa ttttaccgat ttcagcatca
      721 ttaaaacctg caaaataata aagtaggata atctttcttc ttatctccga taactgatca
      781 attgctgctg ataacttttg atctgaaatt aatatttcca ttccaaataa gaaatagaca
      841 tcttgttcat caaaatgagt ttgggaatac ctgccttcat ttgaatctgc caatggttcc
      901 tcattatcct ttatgttagc gtttcgtttt cgaatagact tagcttcgtt tcgtaatact
      961 tttttacagt aggcatcaaa ttggtgttgt atagatatgt cattgtaatg ttgttgcatg
     1021 ataatatgct cctttttccc cctttccact aatacaacaa tcagaaagcc cctttttttc
     1081 gaaaaaacta gaaaaaaaac ataaaaatat ttatcattct ttaaacagaa aatctttcta
     1141 taaaaaacaa aaaaaaacga ttaggctagg ctaatcgttt tttgaacatt cttaaaaaat
     1201 ccaaattatt ttttataact gaaattgggt attgatgacc atcgtttctg tagatagtaa
     1261 gcaatcattt taggttttct atctctagta aagattcctt tcttatttcc ttgcactctt
     1321 aaaatccctt gacttgttgc aaaatctgca aaattccaca cttgttcacc aacaaaattt
     1381 tcacatgtat ctattacttc gtgatttgct tcgtaataag ccacttgata ctcttctgta
     1441 aacattgttg gtactgtatc atgaaatcca gctactgtat ctgctccata ttctgtaaac
     1501 attattggcg tatcaggaca tctttccatc catccatcta gctctttttt caacagatat
     1561 tttgcatgtg ccagatcccc tcccgaaaca taccaaccat aataacgatt taaacataaa
     1621 acatcgacaa tatctcctac cttacaaaca tctggcgttg attttagata agtaacgatt
     1681 gttacaggtc ttttctgcgg atcaagttct ttagttaatt caactaaagg ttcaaaatat
     1741 tctttagcac cttcggaatc cgaatctggt tcatttgcaa tagaccacat tacaacactt
     1801 gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg ttttatgaga attttctgtt
     1861 cgtatctctt tccacgtatt atgtttttcc acattatcat ctaataaagt ggccatgaaa
     1921 tttaaatgta accctactgc aggtacttca tcaataacta caataccttc tcgatctgct
     1981 aatcgcataa tttcttctga gtaaggataa tgtgatgttc taaaagaatt agcacctatc
     2041 catttcatta aattaaaatc tttaatattt acagcctcat tcaaaccacg tccattaaca
     2101 tacgaatcct catgcttacc aaatccttta aaataaaaag gtttattatt aatcaaaaat
     2161 ttgccattgg taacctgaac actgcgaata ccaaattctt ctgtataggt atcgatcact
     2221 ttattatcat caattaaact aattttcata ctatataaat aagcgtctag aggttcccaa
     2281 aaaattggat tcttaagctg aatcattcct ttctttcctt cagcttcagc tacaagttga
     2341 gcctcttcgt catatatcgc tattaagatt tttccggtcc attctgtatc gatattgaat
     2401 tctacacttg catccaaatc agttattctt gtattaatag taatatcact tatatagtct
     2461 ttcggagttg tatataactt aacaggtcta tgaatacctg cataattgaa gaaatcaaaa
     2521 ttaactaagt tttccttttt tatatcccca ttttcctgta ctgtttcctt cataatcccc
     2581 acaggtaaag tactatcgtc aataatattg tttacagcaa ctgtaattcg attattcccg
     2641 tcttttacaa accgattaat ttcagactca aatggtgtaa acccaccttt gtgttgagta
     2701 ataaactttc cattcaaaaa aacttttgct tcatgtgttg cagatccaaa acgaagaact
     2761 aatctttctt ctaataatct tttaggaaga gtcacttctt tttcatacca cacccatcca
     2821 acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaggg aactgaaatc
     2881 ggataactct ctttttcatt tattggttct aaagcaagtt cttcctgtaa gtcatcattt
     2941 gtatctaatt taaatctcca gagaccgctt aagtcaatta cttgcctcgt ctctgtcata
     3001 atgggataca acatata
//
LOCUS       DASJTM010000181         2984 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJTM010000181 DASJTM010000000
VERSION     DASJTM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.184"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            658..>2984
                     /gene="esp"
                     /locus_tag="U4B29_002937"
     CDS             658..>2984
                     /gene="esp"
                     /locus_tag="U4B29_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN2107820.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1090 a          449 c          580 g          865 t
ORIGIN      
        1 acaagttaat agattataag tatttgagtg aaagcaatta attattcatg tagagtacta
       61 gacactttaa atatggacaa ttcaattata aagaaaaaga atcaataatt taatgaggct
      121 gtgacaaatg tcattttttg tttcgctaaa aacgagtgaa aaaatgttga taactccaat
      181 cattcgttta agattctagg aatcattcag aaatgctgtg aaaccgatac tttataatta
      241 taaagaaggt gatttcaaat tccgaagaag gaatttgaag tcaccttttt acttatgtca
      301 cagtctcatt cagtaatgcg ggataaggct ccttattccg ttatttgaat actctgaagt
      361 ttaatctaag aaagggtaat attttgaaaa catgcttcat cgcatagaaa tgaacttaat
      421 ttcagaaaga atacatacat atctttttat atgttttcag ctgctgaatg gttaaaaagc
      481 acaaatatcc gaaaaaaaca tgaagaaatg tacttttttt gttgtaatgt tcatgaaatt
      541 ctctaagtaa attagttaac ataaggttag taagattcct attctgtgaa taatacgtat
      601 cagatttatc ttttattctt actaggaaag taattattaa gggagttgat ttgaataatg
      661 gttagcaaga ataataagag agtatttcta gaaaaaacaa agaaacgagt attgaagtac
      721 agtatcaaaa aattaagtgt tggggttgca tctgttttga ttggtgttgg tcttgtcttg
      781 ggaacaactg aactagttaa agcacaagat gaaatttctc caagcagccc acttgaaaca
      841 gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat
      901 cctggttata cgaaaaatta taacttttcg gatttacaat ttagtcctca agaactaact
      961 ggagacacac taaaaggcaa tacaattgga tttgaagttt acggaaaaca taatattgca
     1021 gcatcaacta aaaactggga aatccgtctt caattagatg aacgattagc taaatatgta
     1081 gaaaaaattc aggtcgatcc aaagaaagga attggatctt ctagacggac ctttgtaaga
     1141 attaatgatt cgctgggtag acctacaaat atttggaagg tcaattatat tcgtgcaagt
     1201 gatggattat ttgctggggc agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt
     1261 acgtttgaaa agagcctaga tgaaattttt aaagagatag gcatagacaa tctcaagacc
     1321 gatcgtttaa tgtatcgaat ctacttggta agtcatcaag acgatgataa aattgtaccg
     1381 ggtattgata gtacgggcta ctttttaaca gattcagatg atttctataa tagcttagat
     1441 gtatctgaaa ataatcctga ccagtttaaa catggatcag taagtgcaaa atatgagaaa
     1501 cctaatactc aaacaaaaga tggttcagga tccacaggtg ctaatggagc tataatctta
     1561 gatcataagt taacgaaaaa ttataatttc tcatattcat cttccgcaaa gggcacgcct
     1621 tggtatgcta attacaaaat tgatgaacga ttagttccat atgtagcggg tatacagatg
     1681 cacatggttc aggctgacaa agtaacatat gatgtttcgt ttgaatccgg aacaaaagta
     1741 gctgatttag caatcgagcg acgggaaggt catgaaaatt atggcatagg ttcaataact
     1801 aataatgatt taactaaact tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt
     1861 cgatatgttt tacaattgac taagccatta gatgaaatct tagaagatat gaaagcaaca
     1921 gcacaagttg aagaaaataa accatttggt gaagatttca tctttgattc ttggttgtcg
     1981 gatacgaata aaaaattaat ccagaacact tatggaacag gttattatta tttgcaagat
     2041 attgatggtg atggaaaccc tgacgataaa gaagagagcg gagacacgaa tccatatatc
     2101 gggaaacctg aattagaaga agtatatgat gttgacacaa cagttaaggg gaaagtattc
     2161 atccacgagt tagcgggaac aggtcacaaa gcccaacttg ttgataaaga aggtactgta
     2221 ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg ctccaatttc agatactgta
     2281 gaatttgaat ttacgggtgt agattcaagt aaattaatcg cgaaagatga attaaaaatc
     2341 caaatcgttt ctccaggttt tgataaacca gaagaaggtt caaccgttat taaggaatca
     2401 ccaaaagcgg ttgataaaca aaccgtggta gttggattta aaccagatgc taaagaatca
     2461 attcggaata ataaaaactt acctgaagat gcagagtatt catggaaaac agagcctgat
     2521 acttctaacg ttactgatag tacgaaaggt attgtaactg ttaagatcgg aaatcgaact
     2581 ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca
     2641 tacgtaccta taacaacaac tccagaaacg acagttcaaa gtggaaaacc tacatttgat
     2701 aaaccagatg ttcctttagc taaagatgcc ttttcaattt cagatgtcta taataagtac
     2761 ttcggtaatg caagtgttga tgcaaacact ggtattgtta cattcactcc agctaaaggt
     2821 gtaggagaat cggagccgat tactggaaca attcctatta aaattgttta ccaagatggt
     2881 tctgtaggca cgaccgattt agcagtaact gtaagtaaag atatttatga aaatccagga
     2941 gaaaacattc ctgcaggcta ccacaaagta accttcaccg cagg
//
LOCUS       DASJTM010000182         2900 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJTM010000182 DASJTM010000000
VERSION     DASJTM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.185"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            116..931
                     /locus_tag="U4B29_002938"
     CDS             116..931
                     /locus_tag="U4B29_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107821.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1057..1314
                     /locus_tag="U4B29_002939"
     CDS             1057..1314
                     /locus_tag="U4B29_002939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107822.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1566..2138)
                     /locus_tag="U4B29_002940"
     CDS             complement(1566..2138)
                     /locus_tag="U4B29_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107823.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2154..2759)
                     /locus_tag="U4B29_002941"
     CDS             complement(2154..2759)
                     /locus_tag="U4B29_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN2107824.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          946 a          528 c          451 g          975 t
ORIGIN      
        1 aaaaataatt actatctagt acgaaattaa ttacatagga taatatatta aggtgctgga
       61 ctacaagtaa aaaatactgt atgatcatga cagattcaag aaaggagcaa taagattggt
      121 taagatagca ggactttcta aaggtggaaa aaccgtttca caactttata aacaaaacta
      181 cagagactgg agacagttca gaaaaaacat taaggattct tactttattc taccaacaga
      241 acttgaaaaa tatttaccga ataacaaagc tattaattta tatctgtatt actgttttaa
      301 ggcaaagaat gaaggcgggg attcctggca ttcggtcaat accatagcag agtctctagg
      361 agtgacaact aaaactataa acacctggaa taaaacctta gttgatttag gtatgattgc
      421 acgtatagac gataaccatc tcagtaaatc gacaatttta cttcctctct ctagttacta
      481 taaattagcc cttgatagtt ctttaaagga tgttaccgag agcactgtcc atgaaattga
      541 tggcaacttg gttgcagttt atcatttgtt tgaatggaga aaaaatgagg ctgatgaaaa
      601 ttataacgtt ccttataaca cactctgttg tgtttttaaa agaaaaacac aaaaagatac
      661 tatctataaa tttatcttgg tccaagatga gaaaataagt aatttcgaat taagcactcc
      721 ttctagtaag atttatgacg atgcatatag atttgaatgt caagatctag aagccctcct
      781 ttccgcaaat aatctaaaca atatagaaca tgaaaacttt gttgtaaaca ctagatttaa
      841 tttagtttct gaaaaggact cagatttgtt agatttgctg atagagctta ctaaaaatct
      901 agataagaaa gaaaacatta ctgtactata actagcatcg gtccatcatt aagtttggac
      961 tattagaaca cttgctatta cagaaaaagg tatgctagcc acgcttacta tttattggta
     1021 tcaaacaagt atcttaattt gaaaggaaga tgatttatgg ctaatttata tatagagaac
     1081 aaaggagact tgaatgccat tattacttta atcgtagtta tggtaaaatc aataggtaat
     1141 atcacaatag gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt
     1201 tggttcttaa atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca
     1261 atagagtcaa tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga
     1321 cttacgctga acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg
     1381 atggtatcct aatatagtaa gcgtggctag cataccttta attactttac cttttacagt
     1441 taccatacac attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca
     1501 tgctaataga ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt
     1561 cttttttaac tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg
     1621 gttatagctt caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc
     1681 ttcttcggtg tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt
     1741 gcaaacattt ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca
     1801 ctcaacaacg tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc
     1861 aaaaccttga tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc
     1921 gtgttacgag caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca
     1981 gccaacagct gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa
     2041 atattcttct tctctacgcc gttctcaagc aactgttgaa tttgttcttt tgtactttga
     2101 gaagcggttg agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa
     2161 tcatctatcg tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata
     2221 tataactcgc gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc
     2281 aaatcgttta tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc
     2341 caatctacaa ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt
     2401 cggccatttc cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc
     2461 tcttcaaaag aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata
     2521 actggtgcaa atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa
     2581 tcgtaaatat ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact
     2641 tcgatttcat ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt
     2701 tcttcaacct tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg
     2761 cgaaatctcc ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc
     2821 gattttgttt atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt
     2881 ctctattttg cacagtctag
//
LOCUS       DASJTM010000183         2888 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJTM010000183 DASJTM010000000
VERSION     DASJTM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.186"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            151..2385
                     /locus_tag="U4B29_002942"
     CDS             151..2385
                     /locus_tag="U4B29_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2107825.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2533..2853
                     /locus_tag="U4B29_002943"
     CDS             2533..2853
                     /locus_tag="U4B29_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2107826.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         1027 a          533 c          591 g          737 t
ORIGIN      
        1 taagagtcaa aggtcaggaa tggtcaatag agatttcacc ttttctacct cataaatctc
       61 ttaaaaaact aggggttatt cttttacctc atgaaaaacg agatgtaaaa tatggatgta
      121 cccatttttg tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg caagaatgag
      181 gcaacgattc acttgtatgc cactgtcaat ggtcagcgga cacaactcga ttactgtcag
      241 agctgttatc aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat ggcccaaaat
      301 gatccatttg gatttggcag tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa
      361 cagcaaggaa attatgagca aacaccacca acacaatttg gcggaaacaa caacttcaat
      421 ggtggtcagc cacctcaagg caatacagaa ggtttattgg gagaatacgg aatcaatatc
      481 acagaacaag cacgacaagg tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa
      541 cgagtcattg aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat tggagaacca
      601 ggtgttggta aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta
      661 ccgcaaaaat taatggacaa agaagtcatt cgtttagatg tcgtatcttt agtccaaggt
      721 actggtatac gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa
      781 gcagaaaatg tgatcctatt catagatgaa gttcacgaaa tagtcggtgc tggttctgct
      841 ggtgatggaa atatggatgc tggtaatatt ttaaaaccag ctttagctcg aggagaattg
      901 caaatggttg gtgctactac cttaaatgag taccgcatca tcgaaaaaga cgctgcttta
      961 gaacgtcgta tgcaacctgt acgggtagat gaaccaactg tcgaagaaac gatcagtatt
     1021 ttgaaaggtt tgcaaaaacg ttatgaagac tatcatcatg taaaatatac tgatgaggcc
     1081 atcgaagcag ccgcaacttt atccaatcgt tacgtccaag atcgcttctt gccggataaa
     1141 gccatcgatt tgctagatga aacaggttct aagaaaaact tgacgatcca aatcgttgat
     1201 cctaaaacaa tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt agcttctcga
     1261 gaagaagatt tcgaaaaagc agcttattat cgcgatcaga tcaataaact tcaaaaaatg
     1321 aaagaacgtc agctcactga agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa
     1381 atcgttgaac aacggacagg tattccagtg ggtgaattaa aagaaaaaga acagacacaa
     1441 ctgaaaaact tagcagatga cttgaaagct catgtcatcg gacaagataa cgctgtagat
     1501 cgagtagcga aagcgattcg ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc
     1561 ggctccttct tatttgttgg accaactggt gtaggtaaaa cagagcttgc gaaacaactt
     1621 gcctacgaac tattcggttc acaagattca atgattcgtt tcgatatgtc tgaatatatg
     1681 gaaaaacata gtgtctctaa attgattggt tctcctccag gttatgtcgg atacgaagaa
     1741 gctggtcaat taacagaaaa agtacggaga aatccttaca gtcttgtctt gttagatgaa
     1801 gtagaaaaag ctcatccaga tgttttgcat atgttcttgc aaatcttaga tgatggtcga
     1861 ttgacagatg cacaaggaag aacggtcagc tttaaagata cgatcatcat tatgacaagt
     1921 aatgccggaa ctggtaaagt agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt
     1981 actcgttctg tattgaacca attaaacaac tactttacac cagaatttct gaaccgtttt
     2041 gacgggatca tcgaattcag tgcattatca aaagaaaact tgatgacaat cgtcacacta
     2101 atgctagacg atgtcaacca aatgctggca gcacagcaac ttcacatcga agttcctacg
     2161 aatgtcaaag aaaaattagt tgacttaggc tatgatccat ctatgggtgc tcgtcctctt
     2221 cgacgcacga tccaagaaca aatcgaagat ggtatcgctg aattttatct tgatcaccct
     2281 tctatccatg agttgaaagc aaaactggat aaagatggaa aaatcatcgt tacttctaaa
     2341 ccagaacgtt tggctaaaga atcagccgaa gaaacggctg aataaatcca gttttatagc
     2401 gtgagacggt atttctttga gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac
     2461 tgttcatttt ataaaaaaga aattataata agagaaacaa gcaattaaaa atacataaaa
     2521 gaggtgagcg ccatgaaatt ggtaaatgta acaaacagcc attcccgtct tgtattaaac
     2581 cagcttgaaa acactgatgc ccatctagtc aaggtctaca cagccggaaa taccactatt
     2641 gtctacactg aagcccctca acataatgaa attctgctga tcaatgacaa acgtaaaatc
     2701 cagccgaaag aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa agacgccgtt
     2761 taccacgagg atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca
     2821 aaacatgaat taggcgctac attagccgtg taaacaaatt ttgaacataa taattaaaga
     2881 gcgtggga
//
LOCUS       DASJTM010000184         2846 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJTM010000184 DASJTM010000000
VERSION     DASJTM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.187"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            243..617
                     /locus_tag="U4B29_002944"
     CDS             243..617
                     /locus_tag="U4B29_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107827.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            622..1050
                     /locus_tag="U4B29_002945"
     CDS             622..1050
                     /locus_tag="U4B29_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2107828.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            1100..1753
                     /locus_tag="U4B29_002946"
     CDS             1100..1753
                     /locus_tag="U4B29_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107829.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            1889..2455
                     /locus_tag="U4B29_002947"
     CDS             1889..2455
                     /locus_tag="U4B29_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEN2107830.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            2571..>2846
                     /locus_tag="U4B29_002948"
     CDS             2571..>2846
                     /locus_tag="U4B29_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2107831.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYY"
BASE COUNT         1073 a          464 c          541 g          768 t
ORIGIN      
        1 cctattagct tttaagctat tatgaataca tagttaaata agacttataa aaagcctcta
       61 aaataacatt tctaagtttg gcgaccgaga gaatgtttta aattagtaga gttttttgtt
      121 gctcttattt agctaacaat ttagcttacg aattaaatat actagcttaa aagctaattg
      181 tcaaccaaaa atataacttt taagctattt attttctttt cagctttgaa aggttgataa
      241 taatgagttt agttactaag attaaagaat tagcagacga aaagcatgtg actatagcag
      301 aagtagaaag acaggtgggc atctctaatg gacaaataag aagatgggat aaagcctcgc
      361 caaaatctga gaacttaaag aaagttgctg attattttgg tgtcacaact gattatttat
      421 tgggaaataa taatgttccc aagtgggcta caaaagagga agtggttgaa cttgataaac
      481 tactagactc aaatgttaat atgtcttatg gtggggaaac attgacaccc gaacaaatac
      541 agcgcgtaaa agatatcctg atagcgactt tctgggatat tgtgaaagaa gacaaagaaa
      601 aaggcaaaaa gatgtgagct tatggagatg gatacgatta atttagtcga ggagttgaag
      661 cggaaatacc agtccgctaa tcctttttat atttgtgaaa agatgggcat taaaattcaa
      721 tacgttcctt ttatcgagaa tcccaagggg cagtttcaag aaattagaga tcgtgcgata
      781 atctttttaa atgatgaact gcgagactct gaggaaagat tctacatttg cgctcacgaa
      841 ttaggtcacg ctatttttca tcgtggctta tccagctatt atgtatcaac aagaacatct
      901 agaagcaaat ctgaaagcga agctaattgc tttgctgcta atctcattgt ttctctttat
      961 aaagaagata atgatcaata tcctaaaaaa atcgaggaat taaagaatct ttacgggctt
     1021 ccagaaagcg cttatcgttt tcttatataa aaaagcccgt gttagcacac atattacaac
     1081 gagaaagagg aattataaaa tgaaaaaagt tagcgttatg ttgttgttaa gtactgctct
     1141 gctactttca gcttgttcaa ataataaaaa agctgaatca acagatacca cttctaacca
     1201 agaaacaaaa ataagtaaaa caaaagaaac aactgaaacc agttcatcta ctagcaaatc
     1261 tacatctaaa acagattcta gttcaacagt tacaagctcc aaccaagcta cggcggaacc
     1321 tagcccaaca gttataagct ccagtcagag tacaatccaa accgcacctc aagaagaaac
     1381 atatgaacag atgaaacaac gcactttaca gtcaactcca gctgatcgtg caaattggtc
     1441 caacaaagag tgggaagctt tcggcgtggc cctttatgaa aatggattga ctacagatga
     1501 tgctggcaat attatcagtc aagatcagaa agaacaacaa gcagcatctc aacagaatcc
     1561 agaagaccaa caaacaagcg ctcagcaaga cgctgacact ttatcactta ctgattttgt
     1621 taacaaatac gggatgtcgc ctgttgcatg gaaagtacag aatggaatgt ctgaagaaga
     1681 agcattgcgt acaacacagc aaaagacttc cggtgaagtt caattaggat tttctaaata
     1741 cggaattcaa taatacattt ttatgcccta ctattttgcc tataatctct aaaaaagtta
     1801 taaagaaaaa agcccgtgct gcaacacgga ctcatacctc atttctgaga tcacaaatat
     1861 attataacaa gaagcgaggg atatttaaat ggcaaaaaaa gttatgggtc aagatgggaa
     1921 aatgtataaa gttaagaaac cgttttataa acgggtatgg ttttggttgt tagcggttgt
     1981 tgtggtgttt attgctatag gttcgcaagg aggcagtgat gatgctaaaa ataccgtcgc
     2041 tgaaacaact aaagaaagcg tgacagaagt gtcttctgca gaatcggtag cagaatctac
     2101 agtcgttgaa gaagaaactg aaactactga aactactata gaagaagtta ctcaagaaga
     2161 aagtgttcct cgtgaataca gaaatgcatt gagcaaagct gaaagctatc taggttgggc
     2221 tggtatgtct gaacaaggtt tgcgtgaaca actagagttt gaagaatatc caagtgatgc
     2281 aatcgattat gcgctggcta atgttgatgt cgattacaac gaacaagctt tggctaaagc
     2341 ggaaagttac gatgattggg catcaatgtc agattcgcaa ttgtacgatc aacttatatt
     2401 tgaaggtttt acaaatgagc aagcacaata cgctttagat aacctaccac aataactaac
     2461 aaaaacacgc ccctccctcg ccaaagtttg tggacgtgat gaaaaataaa cctgctataa
     2521 tcggcttact tatctattcc tattatagca acaaatagga gatgaaaaca atgtggatcg
     2581 aaactaaaac tgataaaaac ggaaaaaaag tatataaata taatgagcga tatattgatc
     2641 caaaaactag aaaaagaaaa aaagtgagca ttacttataa aaataaatct cgagaaactc
     2701 agaaagtggc attgctagag ttaaataaaa aaattgatat aaaattaaac gaaaaaacac
     2761 ttcataagcc tgatctaaca tttcatgagc ttgttgaaga atggttagtt atatacaaaa
     2821 gacaagttaa ggaatctaca tattat
//
LOCUS       DASJTM010000185         2807 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJTM010000185 DASJTM010000000
VERSION     DASJTM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.188"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(147..1085)
                     /locus_tag="U4B29_002949"
     CDS             complement(147..1085)
                     /locus_tag="U4B29_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2107832.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1576..2793
                     /locus_tag="U4B29_002950"
                     /pseudo
     CDS             1576..2793
                     /locus_tag="U4B29_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          992 a          471 c          447 g          897 t
ORIGIN      
        1 ataatattaa aagtcatata taacttcctc ctaaatttta aatttttata tttaggagga
       61 ataatcctct gattttttca tacgttatgt cacctcgtaa atattaatta tactgaatta
      121 gcaattttta tcaaataaaa cttattttac ttccaaaacc taaattcacg ttgccaaaaa
      181 tcaatctgct tttgcaattg tttttcgttc gcttttaaag tcgatttcat taattccgtt
      241 aaatcaattg gagatatttc tctaatcaat tttttaaatt tagtcttagt attcttactt
      301 agctttcccc acatactttc ttcatgcaac aaagtataaa ccatagcttg ctcattaatt
      361 ttttctaaag tagcccacgc aggtttcaag atgtgtaaat cattaaaaca atcattccag
      421 taatcaacca tatctctttt taattcaact tctacacgcc ataaatgttc agacacaact
      481 tcaacatctg cgttatcttt acgttcttgt tttttattat aaattctaat aaatctatca
      541 ctatcacgga caccaaaata ttttgtttct ggcttgccat tacgaccata aaaaacagtt
      601 ttcttaactg ctttatcagt cattgcatag taatcgctca aatcatcttc aaaatcaaaa
      661 gctaagtcta atcttgtaaa accgtcatct tccatgtagt cgataatatt ttgttttaac
      721 caaatcattt cttcatgtgt gagtttattg ggattaaatt caacacgcat attacgtcta
      781 tcccaagtat ctgcttttac tttgtcatat tcgatataaa ctttttcttg aagtgcctta
      841 gctttaaact ttgtttgaag tatatcccaa agtcgtattt gtggctctac actcataaag
      901 tcagatagct ttttagcatt agttttgttc aaatttccaa cgattgtcat ggcatcaaaa
      961 cttaatgcgg gttgagattt tcccaaagtt tgaccactta accggctatt acttaaccgg
     1021 ctattagaga cggccggttt ttttagtgag atttctgcag ctaatttttc atcaattttc
     1081 ttcatttttt atcgctccta aaataatttt tggaacgaaa aaaggacgag tggaatcgca
     1141 aaataaattt cgcctgtatc cattcgtcca ttttgttttg tattcaaaat caaaaattga
     1201 tatactaatc atcgaatgaa taacaagtgt ggtttgttat tccgggcgag aacgttggta
     1261 gcgttgctcg tcttttttta tgtctttttt tcgttaaaac tattttaaaa ctaaatatat
     1321 cttaaagcaa ataaattcaa ggggctaaaa gcgcccctta aaaacccctt gcctctgaaa
     1381 agttttgtgt ttcctttccg tcggtttcaa aagctaattt actcaaaaaa atagatcaag
     1441 ggtaaatgca ctcactgcgt tcgcccttga gctattttat ttcgcaaatt ttaacgcatt
     1501 tgccgacgaa aagataaaac acataaaaat atacgcagat atatttaaaa cgtcttagaa
     1561 cgcaaaattt tacgtttaaa agctatgcaa ggactgtatg aagcaaaatt attgagttat
     1621 ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa agcgaatttt
     1681 ggcaaatata gcggtttttt aggacttgat cttgaaatgg ttcaaacaga gcctagaaag
     1741 cgttatgtgg acggtagtaa ggtacaggaa caccacgcca ttatcccaac aaaacaagta
     1801 cctaccgaat ctgcattagc gaaaatggac gatttacaac gaaaaattta tgctttagtc
     1861 gttaaaacga ccgttgccat gtttttacct gattacttgt atgaagaaac caagatacaa
     1921 acgaaagtag ctgatttgct gtttcaatcg atcggaaaga caccaaagca agaagggtgg
     1981 aaaattcttt tcaaacaaca aaccaaagaa gaaaaagagg acgttcaaac gttaccactc
     2041 gttatcattg gcgaacgtgc cgaagttggt gttaagagtg ttgaaaaaga aacgcaaccg
     2101 ccaaaagctt ttacagaggg tacattatta actgctatga aaacggcgaa taaaacggtt
     2161 gatgatgaag aagcaatcaa gattttacaa gaagttgagg ggattggaac agaagcgaca
     2221 agagcaagta ttattgaagc gttaaaacaa aaagaatata tccaagtgat taagaataag
     2281 cttgttgtaa ctgaaaaagg aaaattattg tgccaggcag ttgaaagtca gcacctttta
     2341 acgagtgctg aaatgacggc taaatgggaa acgtatttaa aaaaaatcgg taaaagagaa
     2401 ggcaatcaag agaactttat tacgaatatc aaaaaattca ttgttcattt actggaagct
     2461 gtacctaacg atatagaaaa actaaatttt tctgattacc aggaacagaa agaaaaagaa
     2521 gcagaaaaaa gtattgtagg aaaatgtcct aagtgtggca acaatattgt attaaaaaaa
     2581 tcgttttatg gttgttcaaa ttatcctgaa tgtaagttta ctttagctga acattttaga
     2641 aagaaaaaac taaccaaaac gaatgtaaaa gaattactgg agggaaaaga aaccctggta
     2701 aaaggaatca aaaacaaaga gaaaaagccc tacaatgccg ttgtaaaaat tggggaaaag
     2761 ggatatattg attttatctc tttttcaaaa taaacataaa agccctt
//
LOCUS       DASJTM010000186         2794 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJTM010000186 DASJTM010000000
VERSION     DASJTM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.189"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2794)
                     /locus_tag="U4B29_002951"
     rRNA            complement(<1..2794)
                     /locus_tag="U4B29_002951"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          582 a          846 c          585 g          781 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccagcagt cctcacgatc tgccttcaac
     1561 gcccttagaa cgctctccta ccaatacacc taaaggtgta ctccacagct tcggtaatat
     1621 gtttagcccc ggtacatttt cggcgcaggg tcactcgact agtgagctat tacgcactct
     1681 ttaaatggtg gctgcttcta agccaacatc ctagttgtct gtgcaacccc acatcctttt
     1741 ccacttaaca tatattttgg gaccttagct ggtggtctgg gctgtttccc tttcgactat
     1801 ggatcttatc actcacagtc tgactcccgg atatgaatga atggcattcg gagtttatct
     1861 gaattcggta acccgagatg ggcccctagt ccaaacagtg ctctacctcc atcattctca
     1921 attccgaggc tagccctaaa gctatttcgg agagaaccag ctatctccaa gttcgtttgg
     1981 aatttctccg ctacccacac ctcatccccg cacttttcaa cgtacgtggg ttcggtcctc
     2041 cagtgcgttt taccgcacct tcaacctgga catgggtaga tcacatggtt tcgggtctac
     2101 gactacatac tcaaacgccc tattcagact cgctttcgct gcggctccgt ctcttcaact
     2161 taacctcgca tgcaatcgta actcgccggt tcattctaca aaaggcacgc catcacccat
     2221 taacgggctt tgacttgttg taggcacacg gtttcaggtt ctatttcact ccccttccgg
     2281 ggtgcttttc acctttccct cacggtactg gttcactatc ggtcactagg gagtatttag
     2341 ccttgggaga tggtcctccc ggattccgac ggaatttctc gtgttccgcc gtactcagga
     2401 tcctcctagg tgtcttccac atttcgtcta cggggttttt accctctttg actgactttt
     2461 ccaagtcatt cgactatctg aaagaactac catattggag tcctacaacc ccaacaagca
     2521 agcttgctgg tttgggcttt tcccgtttcg ctcgccgcta ctcagggaat cgaattttct
     2581 ttctcttcct gcaggtactt agatgtttca gttctctgcg tctacctcga atgtgctatg
     2641 tattcacaca atcgtaacat cctattaaag atgttgggtt cccccattcg gaaatctctg
     2701 gatcatagct tacgtacagc tccccaaagc atatcggtgt tagtcccgtc cttcatcggc
     2761 tcctagtgcc aaggcatcca ccgtgcgccc ttat
//
LOCUS       DASJTM010000187         2746 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJTM010000187 DASJTM010000000
VERSION     DASJTM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.190"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..308)
                     /gene="tnpA"
                     /locus_tag="U4B29_002952"
     CDS             complement(<1..308)
                     /gene="tnpA"
                     /locus_tag="U4B29_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN2107833.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     "
     gene            667..921
                     /locus_tag="U4B29_002953"
     CDS             667..921
                     /locus_tag="U4B29_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2107834.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            922..1239
                     /locus_tag="U4B29_002954"
     CDS             922..1239
                     /locus_tag="U4B29_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2107835.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1798..2733
                     /locus_tag="U4B29_002955"
     CDS             1798..2733
                     /locus_tag="U4B29_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107836.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          969 a          391 c          510 g          876 t
ORIGIN      
        1 atttctttcc aaagaaattg tttgactctt ggaaagtcac gttttatcaa tcttgagctg
       61 gcacttttat aggcattgat gaattttgtc aattctgttt taggttgagc tttgaacata
      121 atatgaacgt ggtctttatc atgattccac tccactaaag taatgtgata cgattctgaa
      181 attctttcaa aagtagtttt ggcataatca gatatttcat catcaatcac ttgtctacga
      241 tatttcgtta ctaaaacaag gtggtaataa agaagaaata ctgaatgatt atttgtatct
      301 aattccatgt tatatcaact catttcttgt atagactgat tgtaacatgg gacaaaataa
      361 aaaggcaata cgccttagtt gtcggtcttc gagttaccaa cggtaaccct cataccgaac
      421 ggcattcatc acccacctat agaggatggg cgacttctgc ctaattctgt taaatggatg
      481 ttaacggaag tgtatggtga gtagttcact cgtattttga tatcgtttgt atatgttttc
      541 agcgttctaa gtggcttatt ttgtccaata ataatatgga gtggaatccc ttaagaaaca
      601 ccgttggcaa ttgttgttat aacatgttat aatttattca tcaaaaacta aaaggagtgt
      661 gataacatgg gaaaagaact aaaggttaga aagatcggca atagcgtggg agtgatcctt
      721 ccaagctctc ttggattaaa aagcggagac attatccaag ccaaacaaga aggcaactta
      781 ttcatattag atactactca aatagcaaaa gaacacgatc gcaaattgat tgaagaaagt
      841 ttccaagact ttgagaaagg tttaacagtt tcagaaattg aaatggtaaa agctttcgga
      901 aaatacggct ggagtgaata gatgaaagag tacacgctag tttattctaa aagttttcag
      961 accagccttg aaacgataat agaagaatgg gaaaatgagc ttgctctttc tccagccagc
     1021 ataaaaagat ttgtaggtgc gatccagaag gctttagaac taaccaaaaa ttttccaact
     1081 atgcatgagg aagtttccga aatctatgga atggatatcc caacatatcg gatcgtgatc
     1141 ggcaaaagct atgccttatt ttatcggatc gaccatgaaa aaaaagagat acttgtagga
     1201 cgtattttcc aatctaaaca aatgaaacta ggcttttaac aataattagg aaaaagtcgc
     1261 tcatcctcta caggtgggtg attaatgccg ctcgatatgg ttgttaaccg tcggttactc
     1321 gaagattgat atataagact tagtaccttt ttattttgct ccatgttatg gccgaaggtc
     1381 taaagataca atcattagtc tagagaaaaa ttaagaaccg taggaactac ggggatcgct
     1441 tggtgaataa gagacactgc tattagtgaa aaaataagat atcaagtacg ctctattccc
     1501 aaaaatttcg tgactttact catgagaggt tcaagtagta tatttttaat caaaattgac
     1561 gtaactttta ataaatacta cctaaataag gtagtattta tttttgtgtt ttaaaagagg
     1621 aagtccaata tatctatggg aaacttctta aatattatgt tggtacatga aaatatacat
     1681 ccctgctaga ctatttacaa cattttactt tgtacgtatt cttgttttaa aaggaatggg
     1741 ataaaatata ggtacaataa ctatttgatg ctaaatataa ttaatggagg agagatagtg
     1801 aacatactta atgaaaaaag atatgtatgg tttgctggta aaaaatatat tactactttc
     1861 aaaagattat ccgagttaat gcagcagact gttaaaagtg ctatgcctga aggatacaag
     1921 tttaattttg ttcaatcttc agtatctact gaaagtatct atttttcaat tctttctgag
     1981 agtaggcttt ttcattttag aataagttct catgtaaaga aaactgatgt tgatatatat
     2041 acgttccgta caaataatta taaaaattta aatgatttga gtagaagtat cagtgaatat
     2101 ctcaagggga atagctatct caagattcga ttttcacata tattaatttt actaccatat
     2161 ttgaatgaga atttcaccct aattcaagta gataagtctt tcattgcctc aggtaatcaa
     2221 ggaaatatga atattacaag taacaaaacg tcacgtgtgc tattggcgtt gatgggttac
     2281 ggaattcttt atagagatga ccaaaatggc cagattctaa taaatcgggc tgggcgttca
     2341 ttattaatga aatatagaaa atttataccg gaagattttc aaaacttaca gttagaaaac
     2401 atgcgtttag aagatttatt tagattagca gaagatgata agtatagatt tgaagttttg
     2461 aaaattcaaa agaatgaact attgaataag ttatatcctg agaatgcgat aaaagcacat
     2521 tttggtgatg ctaatcatat gtatagttgg ttcattccta aaagatttag agggaaaatc
     2581 aatgctggag atcaagtgaa agtactcaat cgaaatagta cagatgtcgt aatgataaca
     2641 gatatctatg tagaagatga agatataatc aaatatatga agcctgtgat aaaacttgtt
     2701 aataaaaata aaggaacccg tggtgctagt taattttttc ctagag
//
LOCUS       DASJTM010000188         2734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJTM010000188 DASJTM010000000
VERSION     DASJTM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.191"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..521
                     /locus_tag="U4B29_002956"
     CDS             75..521
                     /locus_tag="U4B29_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2107837.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            794..2146
                     /locus_tag="U4B29_002957"
     CDS             794..2146
                     /locus_tag="U4B29_002957"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN2107838.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2317..2604
                     /locus_tag="U4B29_002958"
     CDS             2317..2604
                     /locus_tag="U4B29_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107839.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          960 a          449 c          544 g          781 t
ORIGIN      
        1 tcttttcatg ttaagtggat tttaaaatgt aaaaatagta tgataagacg agaataacaa
       61 cgtggggtgt ttacatggaa aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag
      121 agaacattcg aatcacacct caacggtacg cggttttaga atatttgatt gaacatcatt
      181 cccatccaac ggctgatgag atttataaag cacttgaaca tcggtttcca aatatgagtg
      241 ttgcgactgt ttacaataac cttcgcttat ttacttcgat tggatttgtt caagaaatga
      301 agtacggcga tgcttctagc cgatttgatt tcagttcaaa gcgtcattat catgtaattt
      361 gtgaaaactg cggtaagatt gttgattttc attacccagg gcttgaagat atagaaatgg
      421 ctgcaagcag actgactgat tttgagatta atgaacatcg gttggaattg tatggactct
      481 gtccggattg taaagagtca gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt
      541 tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt
      601 tattcacaat cttagatata aaagtgaata ttattaaaat aagaatcttg tagatattgg
      661 gttcttatgc ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta
      721 aactgttgta tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc
      781 ggagggacca taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt
      841 aaaaagtatt ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa
      901 tatttctttc ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc
      961 aggcttgttc tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga
     1021 acacgatgta acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac
     1081 aggagaaaca gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat
     1141 tatcccaccg attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca
     1201 agcaaatgaa attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg
     1261 atatatcggt attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat
     1321 tgatggatta gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga
     1381 aaaagaattg acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat
     1441 cacagatgaa gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga
     1501 tatggtcatt ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga
     1561 tacactgcca aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat
     1621 ttttgcagct ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat
     1681 tcctttggca acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca
     1741 taaaatggcg tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat
     1801 cggttcgaca ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac
     1861 tgtatttgaa gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga
     1921 gctagtctat gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga
     1981 cattacacaa tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga
     2041 tctcgcactt tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa
     2101 cctacttgca caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt
     2161 gacacaattt tgtcacgacc ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc
     2221 ttcgaatttt ttgtcacaat aagcagcaaa gataaagctg ttacataaaa gaacaagcag
     2281 ctttatttga ttttgaaaag aaaggtgaga aagaagatga ataaagaata ttttgtccaa
     2341 caagatgcat ttttagaaga ttataaaagt gcttcgaaga aattaacgat caatattgat
     2401 gagttggcag atcaagtact ctctcagttg aatgaaacgc aagaggaata ttacacgatg
     2461 ccagcaaatg acgccaaaga taatgtttct catcgttttc ctttcagtag acgcatctac
     2521 tcagatgaaa atgacggcac attgaataca gagtacacat atgaaggtca gccatatgtg
     2581 ctagaaactg atgatgatct ttaaagacag tttttgaaaa ggccgctcat ccttaagtga
     2641 tgacaaaaaa tcatcacaat taccgatgac tgtctcttta ctatctttta atcagaaaaa
     2701 ggggttatga caagctttgt caaccccttt tttt
//
LOCUS       DASJTM010000189         2707 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJTM010000189 DASJTM010000000
VERSION     DASJTM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.192"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..759
                     /locus_tag="U4B29_002959"
     CDS             <1..759
                     /locus_tag="U4B29_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2107840.1"
                     /translation="GVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVE
                     LPRVNFYEGLKNGNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLL
                     APLKSDYDLIIIDTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQE
                     QFNPGLDMIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNG
                     ITEHKGYDKKVLSMYENVFFEMLERIIQLENEKE"
     gene            857..1066
                     /locus_tag="U4B29_002960"
     CDS             857..1066
                     /locus_tag="U4B29_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2107841.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1084..1356
                     /locus_tag="U4B29_002961"
     CDS             1084..1356
                     /locus_tag="U4B29_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2107842.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1358..2221
                     /locus_tag="U4B29_002962"
     CDS             1358..2221
                     /locus_tag="U4B29_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEN2107843.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1064 a          439 c          478 g          726 t
ORIGIN      
        1 ggtgttggaa agtccaaatt atcgactatg tttgcttact tgacagacaa attgaattta
       61 aaagttttaa tgatcgataa ggacttacag gcaacattga caaaagactt agcaaaaact
      121 tttgaggtag aattgccacg tgtcaatttt tatgaaggct tgaaaaatgg aaacttggct
      181 tcttctattg ttcatttgac tgataattta gacttgatcc ctggcacgtt tgatttgatg
      241 ttactgccaa aattaactcg ctcatggact tttgaaaatg aaagtagatt gcttgctact
      301 cttttagcac ctttaaaaag tgactatgat ctcattatta ttgatactgt accaacgcca
      361 agcgtttata caaataatgc aatcgtggcg agtgattacg ttatgatccc tttacaagca
      421 gaagaagaaa gtacaaacaa cattcaaaac tatatttcct atttgattga tttacaagaa
      481 caatttaacc ctggactaga tatgatcggt tttgttcctt atttagttga tacggacagc
      541 gcaacgataa aatcaaacct ggaagaactg tacaagcaac ataaagaaga taacttggtt
      601 ttccaaaata ttatcaagcg aagtaataaa gtaagtacct ggtctaaaaa tggaattaca
      661 gaacacaaag gctatgacaa aaaagtttta tccatgtatg agaacgtatt ttttgaaatg
      721 cttgagcgaa ttattcaatt agaaaacgaa aaagaataga atcacaaatc acaagtgatt
      781 aatcacaaat cacttgtgat ttgtgattgt tgatgataaa ataagaataa gaagaaatag
      841 aaagaagtga gtgattgtgg gaaatttagg cgcacaaaaa gcaaaacgaa atgatacacc
      901 aatcagtgca aaaaaagata taatgggaga taagacggtt cgtgttcgtg ctgacttaca
      961 ccatattata aaaattgaaa cagcaaaaaa tggcggaaac gtaaaagaag ttatggatca
     1021 agccttagaa gaatatatac ggaaatattt acctgataaa ctttaaaaag gagtgaaaat
     1081 gaaatggcag ttacgtatga aaaaacattt gaaatagaga tcattaacga attatcggca
     1141 agcgtttata atcgagtatt aaactatgtt ttgaaccatg aactagatac taaaaatact
     1201 cgtttactag aagtgaatct tttaaatcaa ttagaagtgg cacaagaagt tgatttattt
     1261 caacaaccat ttgaagaatt acaagctatt catgagtatt ggcggtcaat gaatcaatat
     1321 tcaaaacaaa ttttgacaaa agagaaagtg gcttaacatg gcgaatatta ctgacttcac
     1381 cgaaaagcaa tttgaagatc gtttagaaaa gaatgttgaa cgactaacta aaaatagact
     1441 agcggttgaa tcgccaaccg cttttttact tggtgggcaa ccaggatcag gtaaaactag
     1501 tttgcgatcg gcaatttccg aagagacaca aggaaatgtt gttatcattg ataatgatac
     1561 gttcaaacaa cagcacccta attttgatga actagtgaaa ctttatgaaa aagacgtagt
     1621 aaaatacgtt accccttatt ctaatcgcat gacagaagcg atcataagcc gtttgagaga
     1681 taaagggtat aatttagtga ttgaaggtac aggacgaaca acagacgttc ctattcaaac
     1741 cgcaacaatg cttcaagcca aagattatga aacaaaaatg tatgtcatgg cagtacctaa
     1801 aatcaactca tatttaggaa caattgaacg gtatgaaact atgtatgcag atgatccaat
     1861 gacagccagg gcaacaccaa aacaagcgca tgatattgtt gtcaaaaact taccgaccaa
     1921 tttagaaacc cttcataaaa cgggcttatt tagcgatata aggctttaca atagagaagg
     1981 agtgaaactc tattcaagct tagaaacgcc ttccattagt ccgaaagaga ccttagaaag
     2041 agaattaaat cgtaaagtat cagggaaaga aattcaaccg actttggaga gaatagagca
     2101 aaaaatggtt caaaatcaac accaagaaac ccctgaattt aaagcaattc aacaaaaaat
     2161 ggaaagcttg cagccaccta caccaccaat acccaaaaca cctaaacttc cagggattta
     2221 aaccttaaaa aagtaaaaga gtagttacca aaaaacggta actactcttt ttttataaaa
     2281 acatttcctc attttttaaa agctgatcga gaaataaagc acacaactgt attctttctt
     2341 ttttcagttg gtacattttg cagtctgtaa cgtgccattt actgtttgtt accttaacat
     2401 atccagtcga aaacacaagg gtatataaac gagcaagctc gcccttgtgt tttctttgtg
     2461 tctatgttgc gttctcgtca cagcaaacga cacgaacaca ctggcgaaaa tgaaccaaca
     2521 aaacgaaaaa agaaaggagc gtacactgta atttatttct agcttttaaa acatgaggaa
     2581 aacagcttca cttcctcaaa cgcctaacaa aaatggttgt caaaaaacta aaaatcaaaa
     2641 aaagaaaaga ggaaaagaaa atggaaaaaa caaaaaaatt acaattagaa gattttacag
     2701 aaaatgg
//
LOCUS       DASJTM010000190         2675 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJTM010000190 DASJTM010000000
VERSION     DASJTM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.193"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..388
                     /locus_tag="U4B29_002963"
     CDS             71..388
                     /locus_tag="U4B29_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN2107844.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            385..546
                     /locus_tag="U4B29_002964"
     CDS             385..546
                     /locus_tag="U4B29_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2107845.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            549..848
                     /locus_tag="U4B29_002965"
     CDS             549..848
                     /locus_tag="U4B29_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107846.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            848..1162
                     /locus_tag="U4B29_002966"
     CDS             848..1162
                     /locus_tag="U4B29_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2107847.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1155..1685
                     /locus_tag="U4B29_002967"
     CDS             1155..1685
                     /locus_tag="U4B29_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN2107848.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1682..1951
                     /locus_tag="U4B29_002968"
     CDS             1682..1951
                     /locus_tag="U4B29_002968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107849.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1881..2084
                     /locus_tag="U4B29_002969"
     CDS             1881..2084
                     /locus_tag="U4B29_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2107850.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2081..2368
                     /locus_tag="U4B29_002970"
     CDS             2081..2368
                     /locus_tag="U4B29_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107851.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT         1006 a          404 c          601 g          664 t
ORIGIN      
        1 cctccaaact ctccaccaaa atcttcctaa atgctttgat accaacgatt aaacagtctg
       61 gagggttaca ttgaaatcag aacatcaaat acaagacgag atcagaattg ccttatcaaa
      121 acatggttgc acgatttttc gatccaatgc gggacaagtg acaacgatcg aagggcggca
      181 attttacggg atgccaaaag ggttcccaga tctatgtgga catcgtaatt ctgatgggaa
      241 agcaatttat atagaagtaa aaaatgaacg cggtaagttg cgtgaagatc aaaaaaggtt
      301 tgctgaattt ttaaacggtc aacctgtact atacggtgtt gcacgatcag tggaagaagc
      361 attgaaaatt gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
      421 agccttcagc gtgagattga cgatctggaa cggacaaagg cagtgatggt caatgaaacg
      481 gcgaggaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
      541 gaatgagcgt ggataagaaa gcagcaatga aacgaatcat cgaactgaca cattctgaga
      601 attggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
      661 gtgaaaaaac caaacggaga acaccgagaa aaattgcaat ctggcatggt gaccgaattc
      721 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
      781 gggatagagc taaacgcgag aacgttgatt cgaaaggtcg tcaatttaaa cattgggaga
      841 agaaataatg gaggaactaa tcacaaaagt agagcagtgg gcaaaagata agggattgga
      901 tcaagctgat tccagcaagc aaatgttaaa aacgatcgaa gagattgggg aagttgccgc
      961 ttctctagct agaaaagatg aatatggttt aagagacgga attggagacg tagtagtaac
     1021 cttgattatt ttagctatgc aaaatgatat ggatttgtac gagtgtctga accaagcata
     1081 caacgaaatc aaaggacgca caggaaaaat ggtagatggt gtattcgtga agtcgagtga
     1141 tttggaggac agcgatgaat aaaaaagtat taattgataa acaagcattg attgatgaat
     1201 taatgaaaat acctggtgtg ggatctaata gtgacgcttt agaaacgatt aaacgtttcc
     1261 catcgtatga accgcagaag ccagttgtgc ctaagtttgt ggcggaacta ctcgactatt
     1321 atcgtcagtc aacagatgtt gatttattag ctttattgat aactttccat gattggtatt
     1381 atcgcaaaac taaagacggt gagcatgaag aagcaattga ttggctagtt gatcatcctg
     1441 aaatttatat gcgtgcatgg ctgtttggct acgagctcga gaaagagccg ttgtattgcg
     1501 taataatcaa tggtagatat cttgtcaaag tattcagcaa tacaaacgtt gtcatattag
     1561 ttccagcaga tgaattttca cagtacgtta ctcaaaaatt tcaattaact gaaaaacaaa
     1621 tcaaagcaat tgatgaaaga tactggccgt ttgctgtgcc agtggatgag gtggtagaag
     1681 gatgagcgac ccatacatta ctaaattaga aaagttgtcc gaagattttg agaagaataa
     1741 aaataaacta actaaagata aacttataga actggttagg ttggttatca gaggagctat
     1801 tacaagagaa aaggcgcagc aaaacataga aatttttgct cgtagtgtcc gagatggatt
     1861 agagaagaaa gttaaacaaa atgaacaaca gacaccgcag aataacaaaa ctaagaaaac
     1921 gggaactgaa tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa
     1981 catataaagt ggcaagtcag tgtgttgctg atgcgagcgg cgctattcgt aagtttggaa
     2041 tttcgatatt aagtgatgat cgtaaatggg aggaaaaaga atgaaactaa aagacggatt
     2101 ttactccagc agtcatggta tcggcggttt aatgctagat atgccgacaa agaaccctaa
     2161 aacacgtgag aaaccaaaat tcaaagtcgg tgacatggtt cgctgtgaag cagaaggatt
     2221 catctatcca ttttgtggat atgtagaaca tctctataat cactcagcaa tcattcgtat
     2281 tgaaaacacg atggaatgtg acaagtggtt agcgaaaagc aaagagaatt tagcagtagc
     2341 gagattggtg gatatggagg ttatataaac agcgaaaaaa tgtacttcta agagaaaaat
     2401 ttatttttta taaaaatagg aaagtatcat tatgaaaaag accacagaga taaataaacc
     2461 taccacataa aaaatgaata tttcttgcga ttcaatatag gtatatttaa caatttgtac
     2521 aataaaaaat actataggta aaaaagataa catgaggagc ttatttgttc tagagtgttt
     2581 gacaaagtaa caaaatgcga cgataccaat acaaacggga atccaaaata acagcatgct
     2641 ccacatagtc actcaaccat ctcctatacg cttta
//
LOCUS       DASJTM010000191         2666 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJTM010000191 DASJTM010000000
VERSION     DASJTM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.194"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(178..453)
                     /locus_tag="U4B29_002971"
     CDS             complement(178..453)
                     /locus_tag="U4B29_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107852.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            685..1419
                     /locus_tag="U4B29_002972"
     CDS             685..1419
                     /locus_tag="U4B29_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107853.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1458..2600
                     /locus_tag="U4B29_002973"
     CDS             1458..2600
                     /locus_tag="U4B29_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2107854.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1012 a          428 c          426 g          800 t
ORIGIN      
        1 ttttcttttt ttgtaactgg cttctctaat aatttttctt tatctacata aattggaata
       61 ttaatcattg atgtttccat ttgtgtttcc tccttataaa attctgttaa agactaaaac
      121 atacagataa taaaaacaaa ctctttatta ctatatgttt tagtcttatt tgtttactca
      181 acctttatac cgttccatga aactatcaat atcagatttt ttaaatctaa tgaccgtatc
      241 aatttttata acaggtaacc cgtattgaga aacatatttt ctaatcgttc ctactgatac
      301 acctagatat tcacttgcct gctgatagtc catatactct agagtgactc cagaaataat
      361 gtcttctagc ataggcttta gcactgtaat tatctctttc acttgttgtt caacttgtat
      421 agaaacaatg ctatcaatga tttgtagaga catatttccc cctcctttcg tgattgcttg
      481 aacaaatttt ctaagaatta tcttcgtatt ttttgctcaa aataaaagta ttttgttatt
      541 aaccgattaa tcaaacaaaa aatgattacc agttcatcga catacacata atacctcgat
      601 aaaaaagagg tgtcaatatc tttgtaaaat ttatttacaa cacccctttt tcgaggtata
      661 ctaattttag gaggtgtggt caatatgata aaacgtccac caataaatta tttagaacga
      721 aaaaagatac tcggaacaaa aataaaagct ataagaaaga gtaaaaaatt aacacaacca
      781 gcatttggtc ttatgattaa caacggtcaa ttaattgaca aaaaaactat ctacgaatgg
      841 gagaagggaa cctatttacc aataccagag cgattatccc gaattgctga tttgggaaat
      901 atgagtatag aggagttagt ttgtggcaat gtagaagaat atattttggg aattattctt
      961 tatagagata gcattgtact agatggtata acatttccag ataaaaacct gttccagcat
     1021 cttcgtcaac agttcccacc agtacactct aatttagata catggctgga tagatattcc
     1081 aaattagaac cagagatgca agaatttatt gctaacaaga cttgtaataa agttaaaaat
     1141 gaaaaaatta gtcttttcaa tatcttaaaa atagaagaat tattcatcaa tgctattgta
     1201 gaagaatttg acaataacat tcttttcctc actagcagta tagaagaact actagaaaga
     1261 atggtagatg aatggctacc tattcaattg aaagacatgt cttatcccga agaagctgtg
     1321 agagaaatta ctgataatat aaacaagcta gaacaaacga tttcatcaat tgggaaaaaa
     1381 tacactaaga aaaaaatgaa gggaggtgat accatctagt ggtaagtact ccttgtaaac
     1441 taagaggaga tgatttcatg gcaaaaatat caaagtacaa aaagaaaaac ggtcaagtgg
     1501 cgtggatgta taaaggacac attgccaccg atccacgaac tggtgaaaaa ataaatacta
     1561 cgagaagagg ttttagtagc aaacaagaag ctcaaaaaga ttatgacgag tacagacatc
     1621 aaatattata cggtgttaaa aagaaagcac ctgatatgac atttgaagac ctgtataatg
     1681 aatggataac tcatcagcgt accagtgtta aagcttcaac aatcgcaatc tcagtaagat
     1741 acgcaaataa tcaaatactt ccagcatttg gtaagcttaa actatcgaat atctctgtcc
     1801 catattgtca aaaagtggta gatgaatggc acagtaaata tgaaagttac gattatatga
     1861 gaaaacagac tgcacaaatt ttacgctacg gggttgcaat gcaatatatt gacaataatc
     1921 ctatggaaaa aacacttctt ccacgaaaaa aagagtatga gaaaaatcgt aaattttact
     1981 caaaagagga gcttaataat ttactggatg cttttaaaga ttttggtaac atgaaacaat
     2041 acgccttctt cagattattg gcatacacag gtatgagaaa gagtgaagta ctcgctttac
     2101 agtggaaaga catagacacg tttaataaag aactacatgt caataaaact cttgctgtag
     2161 atgaatttgg taaagttata attcaatctc cgaaaacaag agcttctaga cgagttatct
     2221 ctttagatac tgagactctt tctatcctca acaactggaa attacagcaa aaagaagagt
     2281 atttaaaact tggatacaat actagctcta aagagcaaca tgtttttaca acagttaaaa
     2341 acactctata cattccaaac actgttaatg attggttaag atatattttg aaaaaatata
     2401 acttgcctcg tattacacct catgggttta gacatacaca tgctagttta ttattagaag
     2461 caggagaatc tgtcaaggta gttcaacaac gattagggca tgaaaatagt aaagtgacat
     2521 tagacatcta tgcacatatt acaaataatg cacctaagaa aacaggtcaa gactttgctg
     2581 atatgatggc tcatcagtaa aagagctggt caaaatctgg tcatttctag tatacaggca
     2641 aaacaaaaaa gctagaaccc ttatat
//
LOCUS       DASJTM010000192         2604 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJTM010000192 DASJTM010000000
VERSION     DASJTM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.195"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..404)
                     /locus_tag="U4B29_002974"
     CDS             complement(<1..404)
                     /locus_tag="U4B29_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2107855.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQII"
     gene            complement(417..827)
                     /locus_tag="U4B29_002975"
     CDS             complement(417..827)
                     /locus_tag="U4B29_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107856.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1102..1314
                     /locus_tag="U4B29_002976"
     CDS             1102..1314
                     /locus_tag="U4B29_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107857.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1317..1529
                     /locus_tag="U4B29_002977"
     CDS             1317..1529
                     /locus_tag="U4B29_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107858.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1669..1824
                     /locus_tag="U4B29_002978"
     CDS             1669..1824
                     /locus_tag="U4B29_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107859.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1821..2045)
                     /locus_tag="U4B29_002979"
     CDS             complement(1821..2045)
                     /locus_tag="U4B29_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2107860.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2123..2311
                     /locus_tag="U4B29_002980"
     CDS             2123..2311
                     /locus_tag="U4B29_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107861.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
BASE COUNT          882 a          414 c          462 g          846 t
ORIGIN      
        1 tcaataattt ggaactgact catatcagat aaatcttgtt catctattgt tctagttaac
       61 atttcaattg caaacatatt agcttcagct tctataccat aaatcataca actaaggttg
      121 tttgctctaa aagctgctgt gcaaatacct ttatggagtc taacgtgggc tatttcatgc
      181 cataatacaa atcttttcat agaaggtgat aggttttgat taattaatat cacataacat
      241 cttttattgt gtatcgtttg tcctaatgtt ttattcccta aatcagtttc aattaaatct
      301 atatcagaat atttgattat tttatatgga ttaatcgcat cagatccaaa ctctacaaat
      361 aatttttcta tttcttctaa tatccagtat ttgtttaaat gcataccaaa gcccacctat
      421 tgacgatatt ttttaggagt gaattttttc tttgctttaa ctttggttaa ggcaagtgca
      481 agttgaatgg catcgtataa tttttcctta tcttcatcgt ctaaaggttc accataataa
      541 tttgtttcag catctgaatt caagccttca agtaattttt cagcttgtaa agcaatatct
      601 tgtttatctt tttctgttaa atcataataa tggcgtttat cagtacgtcc taaaagataa
      661 tcagttgata cattaaagta atctgcaact ttttgtattt tatctccaga tggagtactt
      721 ttattccatc tagacatgct tccatttccg aaatttaact ttctttcaag ttctgccaac
      781 gtcattgaac gttcttttaa taagattttt atacggtcta atagcatagg atatgcattc
      841 ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt atctatttac aaatagagaa
      901 tttgcagtta tactagtttt gtaagcaaga tatttacggc atacaaagac taaaagcaat
      961 aaactaatag tctccccaga catcagtaag ctcgcttttt gtgcgctgta ttttgtatgc
     1021 ttacataata gcatatttgc agtttttgtc aaacttatta gacaaaaata ttagaaaata
     1081 ttcgataagg gggtatgaag catgacttta aaacagcaag tattaattgc tttagtaaag
     1141 aaaggttgga gtcaaagaga gttagctcga cgaatgggta tatcaattac ttatttacga
     1201 gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa aacaaatcga agaacttttg
     1261 gatatcaagt tagaagtaga ttctaatgat cagccatcag aacaggaggc gtaaaaatgg
     1321 tacaacgttt gagcaaagcg gagtttaaag agaaatataa gtattctgaa agtacttatc
     1381 aaagaagaat gaaggaattt aaaaaatcac gttttagtga ggggtataac gcagttactt
     1441 ccaaagaaat aacaattgat gtcgatttat atgaacagtt tagaacatgg cgttctaaaa
     1501 atcgatttag agttagagct gtttgttaat agctaaaaaa tctaaattca ccacttctga
     1561 aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc cacgtttttt cattttggca
     1621 actcctcttc ttggaggtgg tgacggcgag agacgaagga gggatgacat gaaggaagaa
     1681 tcaaaaatag ggattttgaa tgagaacaat tacacaagat taattattga aacagaagaa
     1741 gggaaaaaag tagcagaagt tacattgacc gatgcaactc ctgctgacgg ttatcgtatt
     1801 cgattaactc ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca
     1861 cggctttgaa tttttccatc tctgccatga ataaatagct cagatccttg atttttagaa
     1921 atctctctag cgatttttac tgcttctgat tttgtattag tatgtgcagt cgctttagaa
     1981 tttccagcac ctttgacatt ccaaccacca ttggaattag gtacaacatg ttgatttttt
     2041 cccatagttt tcacctccat ttctatagaa atattttatc aaatagaaat ggagaaattc
     2101 aataaacgaa aggagaagtg atatggcata taccttgcaa caagaacatc aaattctcgg
     2161 tttgattaaa caacgcagga aacaattaca agatgaccgt gcagcgctta gaaaagccga
     2221 tgagctatca gataggcaag ctgaactaat tgcttctgaa cttgaggatt tgagaatgct
     2281 agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt tataggattc
     2341 attttggggt tattagtgat tgtagcgcac caaagtatta tcgggggaag cttgttcgca
     2401 gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga
     2461 agaagcgcta aaaatcggta aagtgattgc tgataatcgg tggggaaata gccgccctat
     2521 tattttaagc aagcaacata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact
     2581 ctgccagcca gcaaagagtc acat
//
LOCUS       DASJTM010000193         2530 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJTM010000193 DASJTM010000000
VERSION     DASJTM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.196"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..454
                     /locus_tag="U4B29_002981"
     CDS             <1..454
                     /locus_tag="U4B29_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2107862.1"
                     /translation="IVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGT
                     VIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGI
                     GSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            444..1586
                     /locus_tag="U4B29_002982"
     CDS             444..1586
                     /locus_tag="U4B29_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2107863.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1599..>2530
                     /locus_tag="U4B29_002983"
     CDS             1599..>2530
                     /locus_tag="U4B29_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2107864.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFE"
BASE COUNT          901 a          327 c          501 g          801 t
ORIGIN      
        1 tattgtagct attggtgact gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc
       61 aagtgagcaa tatggaatag ctgttcatcc aagtgctata gtggcaaaag aaatttcaat
      121 tggttatgga actgttattt ttgcgaatgc tgtaattaac ccagatgctg taattggaga
      181 gcatgctata ataaacacag gtagcattgt tgagcatgat tgtagaatta ataactatgt
      241 tcatttatct ccaggtgtat gcttagcggg tggagtacat gtaggtgtag gaactcaaat
      301 aggaattggt tctcaatgta tccagatgaa gaaaattgga tcttggtgta tgataggcgc
      361 agggagcact attgttaagg atattccctc acattcctta gcttatggca atccagctaa
      421 aattaagaaa gaaggaatta attttgaatt ctagaatttt attatcaagt cctcatatgg
      481 gaggaacaga agaaaaatat gttgctaagg cttttaaaga taattggata gcgccattag
      541 gtgaaaatgt ggataaattt gaagaaagta ttacaaaata tactggttct aagggcggag
      601 cttctgcgtt aagttcaggt acagctgcca tacatcttgc cttaagactg cttaatgttg
      661 gaaaaaacga tattgtcttt gctccttcat ttacttttat agctacagta aatcctattt
      721 tatatcaagg ggctgtacca gtgttcattg actcagaacc taatacatgg aatatgagcc
      781 ctgttgcact agagagcgca ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa
      841 tcatcgtagt gaatttatat ggtcaaatgg ctgattatga acgaatttta gctattgcaa
      901 accaatttaa tgttccagtg atagaagatg cagcggaatc gttaggttct acttataagg
      961 gaatttcgtc aggcttattt gggacaattg ggatctattc atttaacggt aataagatca
     1021 tcacaacctc aggtggagga gctctagtgt ctagtgataa aaaatatgtt gagcattcaa
     1081 gatttttagc aacacaagca agagatcaag caccgtatta tcaacatagc gaaataggtt
     1141 ataattaccg tctaagcaat atttctgccg gtatcggacg tggacaaatg gaagtattga
     1201 atgatagagt agctaaacga agagaaattt tcaatcgata taaggaagct ttttctggaa
     1261 ttgatagaat tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt tggttgtcta
     1321 ctttaacgat caatgattat aattctattg atcaagtagt taacgtaata aataaactaa
     1381 atgaaaaaaa cattgaaaca agagctttgt ggaaaccact acatttgcaa ccagtatttc
     1441 aaggggtaaa aatgtattac catgataata atcttagtgt gtcagaaaag ttatttaata
     1501 gcggcttatg cttaccttct ggaagtaata tgacagatag tgaacaagaa tttgttattg
     1561 atgagtttaa aaaaatattc aagtaggtgt gatattttat gaacatagga attattggtt
     1621 taggttccat ggggaaaaga agacttagac tcattcaaga acactttaat gatattttac
     1681 tatgtggtgt tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac
     1741 caacttataa tagtctaaca gaggcaaaaa atttgtttga aataaattca gtttttgttt
     1801 gtacatcccc tataacccat gaatttatta taaaagaggc tttaaattta gattgtaata
     1861 tctttacgga aatcaattta ttaaatgaat attatgatga aataatcgaa gtagctaaaa
     1921 agaaagatag attactttat ctatcttcta catttttgaa gagaaaagaa attcaatata
     1981 ttaagtctaa agtgacgcat aataaaaaaa tttcttatca ataccatgta ggacagtatt
     2041 tacccgactg gcatccttgg gaagattatc gcaatttttt tgtttcaaac aaaaaaacta
     2101 atgggtgtag agaaatattc gctattgaat taccttggat tatagataca tttgggaaag
     2161 ttaaatcgtt tactgtaatg acccagaaat tatccaatct tgatattgat tattcggaca
     2221 gtttcactct tattattgag catgaaactg gtatagttgg aactttaagt gtagatgttg
     2281 tttcaagagt agctaaaaga aatttaacta taattggtga aaagatttta atagaatggg
     2341 aaggtaaccc ggattctcta ttttcatgga atgaagaaac ttctagtatg acagatattc
     2401 aattgtacga tgaggttatg catgataata attattcaca tactattatt gaagatgcat
     2461 atttagagga gattaaagaa tttttcacgc ttttggaaaa gaaaatggaa ccccaatata
     2521 cttttgaaaa
//
LOCUS       DASJTM010000194         2482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJTM010000194 DASJTM010000000
VERSION     DASJTM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.198"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..727
                     /locus_tag="U4B29_002984"
     CDS             <1..727
                     /locus_tag="U4B29_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2107865.1"
                     /translation="LFFGPIAAEIFDVHHREKFLQDWNNINLYSETRGINRFLGLVKT
                     LEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQSEDLLKALKWSEEVNQQISQAKG
                     LDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLSLVDELCCQDKGKKED
                     IIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKESWENLRTDVLFKC
                     LSGDYVDIEQKYIDTFWKNLTNK"
     gene            740..>2482
                     /locus_tag="U4B29_002985"
     CDS             740..>2482
                     /locus_tag="U4B29_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN2107866.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYC"
BASE COUNT          918 a          299 c          491 g          774 t
ORIGIN      
        1 tctctttttc ggaccaattg ctgcagagat ttttgatgtt catcatagag aaaaattcct
       61 acaggattgg aataatatta atttatattc agagactcgt ggaataaaca gatttttagg
      121 tttagttaaa acattagaaa aaaatgatgt aacatcgatt aatcgattaa agcactgggt
      181 atctctaaca aaagaattgt ctaatccatc tttgaagagg gaaatagaat attctcagtc
      241 agaggattta ttaaaagctt taaaatggtc ggaagaagtc aatcaacaga ttagtcaagc
      301 aaaaggatta gataaaccct ttttaagtgc tagagatact ataaaagctc tgaaaaaata
      361 tgggaagata attattgtaa gctcagctaa taaggaagct gttcaagaag aatgggaaag
      421 acatgaatta ttatcattag ttgatgaact gtgttgtcaa gataaaggaa aaaaagaaga
      481 tattattcga agtgtaattg aaaatggttg tgatttggat aagatattaa tgattggtga
      541 ttctcctggt gatctagaag ctgcaaataa aaataaagtt ttcttttatc ctattttggt
      601 caataaagaa aaagaatcat gggaaaactt aagaactgat gttttattta aatgtttatc
      661 gggagattat gtagatattg aacaaaaata tatagatact ttttggaaaa atcttactaa
      721 taagtagagg agaaagaaaa tgactataaa agtagattta cgaaaaaaac catataattt
      781 agatgaagat gctatttctt ggattgaaga gacgattgat tctatgactt tggaagaaaa
      841 aataggtcaa ttgtttgtta atatgggatc tagtcgtgat aaaaattact tgagtaatat
      901 ggtcaatcag taccatattg gagctgtacg atataatcca ggaactgctg atgaggtata
      961 tgatcaaaac tatatcttac aaacagaaag taaaattcct ctattaattg ctgctaatac
     1021 ggaagcaggt ggtaacggag cttgtacaga tggtactgaa gttggtgtag aagtaaaaat
     1081 tggtgcaaca aatgatgcga aatatgctta tgaattaggg agagtttctg ggattgaagc
     1141 atcggctatt ggatgtaact ggagttttgc tcctattgta gatattaata gaaattggag
     1201 aaatcctatc atttctagtc gatcttttgg acaaaaccct gataaagttt tagaacttag
     1261 tttagcttat atgaaaggta ttcaggagag tggaattttg cctgcagcta aacatttccc
     1321 tggtgacggt attgacgaac gagatcagca tctatctttt agtattaata gtttaggtac
     1381 ggaagaatgg gataattctt ttggaaaagt atactcaggc ttaatagaag ctggattacc
     1441 ttcaattatg gcaggtcata tccatatgcc agcttattca aaacatttta atcctgatct
     1501 gaaagatgaa gatttacttc ctgcaacttt atcaaaagaa ttaatcactg acttacttag
     1561 aggaaaactc ggatttaatg gattagtagt tactgatgct agtcatatgt taggaatgac
     1621 tggggcaatg aaacgatcag aattattgcc cacttctatt gcagctggat gtgatctatt
     1681 tctatttttt aatgatccag atgaagactt taattatatg atggaaggct atcgtaaagg
     1741 aattatcact gatgatcggt tattagatgc cttaaaaaga attttagggt taaaagctaa
     1801 attgggatta cacaaaaaag caaaagaaca aattttatta cctaagcaag aagctctgga
     1861 aaaaattggt ttaattgaaa ataaatcaat ttttaaagag gtggctgacc aagcaattac
     1921 attagttaaa gataaacaag atatttggcc tattgtacca gaaaaatata aaaggatttt
     1981 actagtagat gtacatgggg taaaaggtgg gtttggtgaa gtagttgctg gaaatagacc
     2041 tcaagccatt gatttattga aacagatttt gtcagcaaaa ggatttgaag ttgaaaaata
     2101 catttcacct gaagaagaat tgaaaggatt atcgcaagaa gaaaaaatta aaaatatagc
     2161 tggaatatat gctgctaaga gaccaattaa aaatttaact aaaaaatatg acttgatcat
     2221 taatatagct aatgttaatc caggcacagt tcaacgaatt gtctggccag caagtaaagg
     2281 aactccggat ataccgtttt acattcatga aattccaaca atttttgtat ctgtacagtg
     2341 tccgtttcat ttggctgatg tacctcaagt aaagacttat attaatactt atgatggaaa
     2401 acaagaaact ttagaaatat tggtggacaa actaatgggt ctttctgaat ttaagggagt
     2461 aagtccaata gatgcttatt gt
//
LOCUS       DASJTM010000195         2458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJTM010000195 DASJTM010000000
VERSION     DASJTM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.199"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1149
                     /locus_tag="U4B29_002986"
                     /pseudo
     CDS             5..1149
                     /locus_tag="U4B29_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="U4B29_002987"
     CDS             1320..1778
                     /locus_tag="U4B29_002987"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN2107867.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="U4B29_002988"
     CDS             1937..2407
                     /locus_tag="U4B29_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN2107868.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DASJTM010000196         2368 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJTM010000196 DASJTM010000000
VERSION     DASJTM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.200"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1010
                     /locus_tag="U4B29_002989"
     CDS             <1..1010
                     /locus_tag="U4B29_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2107869.1"
                     /translation="RKVLSDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYEN
                     YRFTIKNHINGYLGKKKLTDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAV
                     YPWGLIKQNPMLYVKIPKYEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGF
                     YTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLL
                     AILKKHKKQQLENRMKYGKLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNF
                     HSLRHTHATLLLENGAKMKEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKN
                     S"
     gene            complement(1228..2361)
                     /locus_tag="U4B29_002990"
     CDS             complement(1228..2361)
                     /locus_tag="U4B29_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2107870.1"
                     /translation="MANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEARRG
                     LEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDFFGE
                     MQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLEIIE
                     ENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVRSEE
                     LLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWK
                     KTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEH
                     NKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          788 a          433 c          377 g          770 t
ORIGIN      
        1 tacgaaaagt tttatcagat attgacgaaa caggacaata ctttttaggt acggatactc
       61 gagtaaaaca ataccttgat ttttggatgg aggaatacgt taaactaaat ctaaaataca
      121 atacctatga aaactaccga tttaccatca aaaatcatat aaacggttat ttaggaaaga
      181 aaaaacttac ggatctctcc cctgctcttt tacaaaattt catcaatgct gaatttaaaa
      241 agggttactc gaagaaaaca atgactatta ctcactctgt ccttaagaat gcgctgaata
      301 tggcggttta tccttgggga ttaatcaagc aaaatcctat gctgtatgta aaaataccaa
      361 aatatgaagc acgaccaacg actaaaaaag atctaaaaat catttctctt gaggactttg
      421 atcatatgct agaaattact cctgaaggac atcctttcta tattcctttg aatattggat
      481 tttatacggg aatgcgcgtt ggcgaagttt gtggtctgac gtgggatgat gtcgattttt
      541 caaatggaac aattactgta gagaaacaaa tggtaaagaa tgacggcgca tgggtatatg
      601 gtacgccaaa gacaagcagt tccaatcgaa cgatttttat tggacaaacc ttgctagcaa
      661 ttctgaaaaa acataagaaa caacaattag aaaatcgaat gaagtatgga aagctctaca
      721 ttgattcaaa tgcagtatgt acgaaggaag acggtgagct agttacgcca agtgtagtga
      781 aatggaatac taggaggata tcgaatgcac tctccctctc ttttaacttc cattctctca
      841 gacatactca tgctacactt cttctcgaaa atggcgcaaa aatgaaagaa atctctgaac
      901 gattgggcca cagcagaatt tcaattacga tggatactta ctcgcatgta acagataaga
      961 tgagaaatga aacggtcgat atcatggaaa atcttagaaa gaattcttga tttttgccac
     1021 cgaaaaaaac caccggtggc aaaccggtgg caaatccatg taaacatggt ttatttttta
     1081 cactgttttc aaacaaaccc ttgctatttc ttcggttcgt cctcgtccat tttcgatacc
     1141 gacagctagc ttataaccca aggtatatca acataaaaaa ataaaaatag gctatgacag
     1201 cagcgtgaca gcaaaaaaga tggaaagtta aatatcaaac ctcgtaattt catttgcgag
     1261 tgcttgattt gtttctggat ataagtgtcc atatttgtcc aaagtagttt taatagaggc
     1321 atgacctaga cgcttagata ttgcatagat atttttatta tgttcaatta aaaaggcaac
     1381 atgcgagtgt cttaatccat gcatatgaat tggttttact cctgcaattt cagctagttt
     1441 ttttattctg cttaaaatag ttcttggaga tggcggtaat ccatcaaaag aaaaaaccaa
     1501 ttctatattt ttcccaattg attgttgagt ttttttccat gtttttaaat cttcaacggt
     1561 ttgtctgtct aacgaaatta tccgaatact tgatgtattt tttgtttcag caaattcata
     1621 atcttgtctt gtacggatgt aaattgactt atttacgcta catctgccat ttattaagtc
     1681 aaagtctttc cattgtaacg ctaagagctc ctcactcctt acacctgtaa caaaaataaa
     1741 acgcatcaaa cgtttataaa attcttcata aaaatccccg tgataaacac tattatatac
     1801 tttcttaaat tcatcaactg tccaaaattc tacttgagga cgctgagttt ttattttacc
     1861 tatagtatct acaggatttt cttcaataat ttcaagtaca attgcccttt taaaaactac
     1921 tctcaactta ttgaatatct gtttaatata atttggtgat aatggttgct caatgccctt
     1981 ttttacaaca tggattttcg ataaaaactg ttgaaattcc tgtatatgaa ttggtcgaat
     2041 ctttacaacc tgcatttctc caaaaaaatc caatgcacgt tgtaaaatat gatctgtttt
     2101 catatatgtt ttttctacag ttcccaactt ataccaagga aaaaaatact ctcgataaaa
     2161 atcatgaaac gttattcctt gcagataaat tccttgtgaa tcagaaaaac tttgttttaa
     2221 ctcttcgagt ccccttctag cttctccttg agttttgaag cctcttctgg tcacttgaat
     2281 tctttttcca aagcgatctt gtcctaaagt tgcagaaaaa aaccaagtgc ctctttttct
     2341 gtctttataa acattcgcaa ttttcctc
//
LOCUS       DASJTM010000197         2349 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJTM010000197 DASJTM010000000
VERSION     DASJTM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.201"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..2018
                     /locus_tag="U4B29_002991"
     CDS             156..2018
                     /locus_tag="U4B29_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2107871.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2018..>2349
                     /locus_tag="U4B29_002992"
     CDS             2018..>2349
                     /locus_tag="U4B29_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2107872.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          844 a          349 c          459 g          697 t
ORIGIN      
        1 aaacgctttt taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt
       61 taaaaagcta taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga
      121 aaacgcttat attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa
      181 ggcagctttt gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg
      241 aaaaagaaaa tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc
      301 tgaatgagcg gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta
      361 gtagctctga tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat
      421 ttgcagatca aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag
      481 ctattactgg aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg
      541 atttagatgt gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc
      601 gaaagggttt ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc
      661 ttcaaaaaga gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg
      721 gtgagactga atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta
      781 atcaagtaat cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt
      841 acgcggaatt actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag
      901 aggctacgat tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt
      961 cttatcgcgt catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat
     1021 atcattatat ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag
     1081 ttgagaatgt gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac
     1141 aattgttgac gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg
     1201 tcaatgaata taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg
     1261 cgatcaagca agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt
     1321 tcgtggcata tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa
     1381 gcgttagagc cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa
     1441 agatcaatga agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc
     1501 aagaaatgat tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca
     1561 tcgatcgtcc tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc
     1621 aacaaataat aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc
     1681 ttagagaaac gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga
     1741 aagcttatac gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat
     1801 atcgagaagc ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac
     1861 aatatatggc agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag
     1921 gaaaaatcga agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg
     1981 cattattctc atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt
     2041 aaaaattatc gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga
     2101 agagcgatta gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa
     2161 tcacttgaat gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg
     2221 ggatttgttc aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag
     2281 tatcggtaat ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa
     2341 aaataatta
//
LOCUS       DASJTM010000198         2348 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJTM010000198 DASJTM010000000
VERSION     DASJTM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.202"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..144
                     /locus_tag="U4B29_002993"
     CDS             <1..144
                     /locus_tag="U4B29_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107873.1"
                     /translation="KKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEG
                     EKK"
     gene            141..623
                     /locus_tag="U4B29_002994"
     CDS             141..623
                     /locus_tag="U4B29_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107874.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            620..1282
                     /locus_tag="U4B29_002995"
     CDS             620..1282
                     /locus_tag="U4B29_002995"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN2107875.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            1269..1637
                     /locus_tag="U4B29_002996"
     CDS             1269..1637
                     /locus_tag="U4B29_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN2107876.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            1718..2170
                     /locus_tag="U4B29_002997"
     CDS             1718..2170
                     /locus_tag="U4B29_002997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107877.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
BASE COUNT          883 a          283 c          505 g          677 t
ORIGIN      
        1 aaaaaatttt attatgaatt tgttgatttg gttgtagaat attctgttcg attgatcgtt
       61 ccgtggagta aagaagaaat taatgaggca ttaaaacatg aacgaaaaag tacattcaaa
      121 gttttagaag gtgaaaaaaa gtgaaaacag accttactag gcaagctgag aagtgcttat
      181 ggcatgaaac aaatcgcatg ggtgtatttg gttgtttcga agtcactatc ggttgggatg
      241 gcagtgaaag agtagattat ataacttatt cagtagataa cacagttcga tgttatgaga
      301 tcaaagttac tttagctgat ttaaaaagca aggcgaaaca gacttttatc ggtgattata
      361 actatttagt tgttactagt gatttgtggg agaaaatgca acaagacgat aaacttatgc
      421 ggaaatatta ctatcaaggc gtatacgtgt tcaatgaaaa atatcctagc ataataagtg
      481 ttaagaaagc aaggaaacaa aaagtatcgc taggtacaag atcaactgta ttagaaagca
      541 tggttcgttc tatgaatcgt gaagttaaga aactctataa agtaaaacca ttttgggaga
      601 tagcagaggt ggaaaaagga tgattcaaac atttgcaaaa atgagtagaa gtgcttggat
      661 tgaaaagctg gaaaatgatt tgagtgttat gcgattagct ccaaagaaat tcaaacgaga
      721 gattgcagaa aaagaagcaa ttttgtcggt attgaatgcg gtagaactgg acgaaccgaa
      781 aaagtcagtt gatgtacctg aaaatgtagc agagttacta gattattatc gtaattcgtc
      841 agatgtcgat ttgttagctc tgatattaac atttcacgat tggtattact gtcaaaatga
      901 agatactcag catgataaac atgataaagc agttgactgg ttaatagaga atccagaaaa
      961 gttcatgcgt gcttggctag atggctatga tagcaagaaa aagccactgg aaagtaatga
     1021 ggaaacgtat ttaacggcag aacaatatga ctatgctaga aaaacaggag attgggaatc
     1081 tggtaagact ttcaatcaca gaaaagatca accaaaaatt agtttttcag atatagccgc
     1141 aattatcgaa ggtatctact atggacaagc cgatgaagag atcttttcta tacaagattt
     1201 attagaattt ttgaacaaaa ataaggatag ctttgtagaa ctaactgatt atgaggagga
     1261 tacagcaaat ggaaaattat gatccaaata tgcgatgggg tacacatacg cttaaagttt
     1321 cttttcaacg ttgggattac aagggatttg tgacttttag aaaagctgga aattgtaaag
     1381 gattagatgt cttagcgttg gatgaagact atctatatga ccatccattg acggataatc
     1441 caatcggatt tggattgctt cctgaagatg atgagggcaa tgaatggttc aaaatgattc
     1501 ttacaaacga taaaggtgat caattatttg tagaagatac ttggtcatat ttgagtgaat
     1561 atattgttag cattaaaatc attgattttg tagcagataa agaaaaagag attggagaag
     1621 gtaaatctaa ttactagcta atgaatagca actttggagg ataagtcaaa agaataaaaa
     1681 aagaatactg tgtgtcattg cagggatgta tctttgcatg atagtatctg cggttaccat
     1741 ttattggtta ggaaatcagc aaaacagttt aattgttgat ctgggggagt taaaggttgg
     1801 attacgaatg actagatggc ttttattagc tcaaacagtt ggtttagcca tattgacttg
     1861 gatatgtgtg gaaaaaataa catctaataa taatcaagag attgttgaat caacaaacct
     1921 aagaataaga ataactgaac aagcaattga aaagcaaaaa gacaaagaaa aaaaggaaat
     1981 aatgtcaatt tatttatcta gtggagcatc attttttcat gaaatagatt cgatgaaact
     2041 gagtagtcaa gaaatcattg atagatggaa aaacatgcct ctaggtggtg agttaatcgt
     2101 tgaagatagt gatggttggt cagcttataa gaaggacgag attgtagcta tcacgatttt
     2161 gtttgaatag taatatatta ccactagata atctgaatat gttgatctgc tagtgacgta
     2221 ccaaatgcgt ttaatagaac cttggtcaca ggcagaaatt gataaagcag taaaattgaa
     2281 ccgattatca aaatttaacg tattaggaaa tgccttttaa aaatcgcaat acacaaacag
     2341 aaaaatac
//
LOCUS       DASJTM010000199         2249 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJTM010000199 DASJTM010000000
VERSION     DASJTM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.203"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2145)
                     /locus_tag="U4B29_002998"
     CDS             complement(148..2145)
                     /locus_tag="U4B29_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN2107878.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          572 a          504 c          411 g          762 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttctta
//
LOCUS       DASJTM010000200         2240 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJTM010000200 DASJTM010000000
VERSION     DASJTM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.204"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1519
                     /locus_tag="U4B29_002999"
     CDS             <1..1519
                     /locus_tag="U4B29_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2107879.1"
                     /translation="KQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNNLF
                     TSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLGLL
                     CIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVSML
                     ITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELEKE
                     TIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAI
                     IYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSD
                     NLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNW
                     FGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYLSY
                     FACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            1697..1918
                     /locus_tag="U4B29_003000"
     CDS             1697..1918
                     /locus_tag="U4B29_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN2107880.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          753 a          236 c          427 g          824 t
ORIGIN      
        1 taaacaaaat gctaaaagga atataattac tggaacaatt tttaagttat atcagattat
       61 cattcccttt tttatgcgta cacttatgat ttatcagatg ggaatggagt atgctggatt
      121 gaacaacctg ttcacatcta tatttcaaat gttaaattta gcggaattgg gaataggtgc
      181 tgcactaact gtaagtatgt ataaacctat agcagaagac aatagaaaag agatttgtgc
      241 tttactgaac gcatataaaa agtacttttc aattattgga tggatagtgt ttacattagg
      301 attgttatgc attccttttt tgagattttt agttaaaggt tcgattcctg gaaatttaaa
      361 tatgacagtt ttgtatttaa tgtatttgat aaatactgtt ttgtcgtatt ggttattttc
      421 atatagaaag agtctaatat atgcacatca aagaaatgat gtaattaacg tgtctatgct
      481 aattactttt acattccaat ttgtaatgca ggcatatgtt ctgattttct taaaaaatta
      541 ctatttgtat ttattggctt ctattttggg acaaatagtt cttaacttaa tgtgtgctgt
      601 tgtttctcaa aagatgtatc ctgattatac tcctgttggt gagttagaga aagaaactat
      661 tgacgatatt ataaaaaaag taaaaggatt agttacaaac aaggtaggcg gtacaatatt
      721 acgatcttca gattcaatta ttatttcatc tttcttaggt ctttcagtac ttgcgattta
      781 tcagaattat tactttatta tttctgccat aatgagtata tttgcaataa tttatgaatc
      841 atgtatagca ggtataggta acagtcttat tgtagaaagt caagaaaaaa attatataga
      901 tttttgtgat attacaacaa ttataggttg gtttctggca attagttgtg catgctttat
      961 tacattattt cagccgttca tgattatctg ggtgggaagt gataatctta tgagtatttc
     1021 atttgttata tttatgatag ttcatttttt cttatatgaa attgatcagt taattgggtt
     1081 atacaaagat gctgctggaa tatggtacgc agacaggttt agaccattaa ttacagcaat
     1141 gataaacatt gtattaaata tcatcctcgt taattggttc ggattatatg gagttttggc
     1201 ggcaaccatt gtttcagtat tgttggttga tttaccctgg ttattatata atgttttcca
     1261 aaatatattt aaatttgaat cggcaggtaa atatgtttgg aagctcataa gtttgtgtgt
     1321 aatagctttt gttgcttctt atttaagtta ttttgcttgt tcgtatgtta atatcggaaa
     1381 tttcaaagtg ataagtttag tgataaattt agttatatcg gttgtcatat cgattttagt
     1441 tattcttctg tttaatatcc gtaataagaa gtttaagtat attttgaaag ttcttacgtc
     1501 ggtattggta aaaagatgag aagagacatc agaatgatat ataaagtaac tacaattttt
     1561 aggctgccga ataggcgaac gatgtatagt ttttgttcca acgaagactc ttgttgaaat
     1621 tggagatgat atgaaggagt tacaattttg actcatgttt atgattgatc ggttttaaaa
     1681 gtagtatgga gaagctttgg gatcatcgga gaggatcaaa ataggtaata atgtttttat
     1741 aggaatgaag tctagaatac taaagggtgt acatatagga aataatgtag ttataggtgc
     1801 aaatagttta gtaaacaagg acgtatcaga cgattgtgtt gttgcaggaa atctagcaaa
     1861 agtaattatg tccctagatg aatattataa aaagataaac aatatgaaga atccgtaaaa
     1921 cttgtgaaac tataccgaga ataatatggg aaaaatattg atgaaaaagc attatatgag
     1981 tttttagggc tgtttacaga tggagatgac aatttgtcga agagttggga gcaatgcaaa
     2041 atttagtagg ggattataaa cagactgaca aggtatttaa agaacatgca caagtatttc
     2101 ataatattga agaatttttg aatactgtga gataggagtt gctacattga gaaaagtatg
     2161 tatttttcca atgtaaaaga ttacgagttt gaaaacgaca ccataatatt attttttaag
     2221 aagatattat agagcttagg
//
LOCUS       DASJTM010000201         2227 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJTM010000201 DASJTM010000000
VERSION     DASJTM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.205"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..280)
                     /locus_tag="U4B29_003001"
     CDS             complement(<1..280)
                     /locus_tag="U4B29_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN2107881.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="U4B29_003002"
                     /pseudo
     CDS             742..2124
                     /locus_tag="U4B29_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DASJTM010000202         2207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJTM010000202 DASJTM010000000
VERSION     DASJTM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.206"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..1007
                     /locus_tag="U4B29_003003"
     CDS             288..1007
                     /locus_tag="U4B29_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2107882.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            1009..1851
                     /locus_tag="U4B29_003004"
     CDS             1009..1851
                     /locus_tag="U4B29_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2107883.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            1851..>2207
                     /locus_tag="U4B29_003005"
     CDS             1851..>2207
                     /locus_tag="U4B29_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2107884.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENK"
BASE COUNT          763 a          267 c          405 g          772 t
ORIGIN      
        1 tttataaatg ttttgatgct tatcaactta ttcaacccgc tatccacttc aagtataatt
       61 tttccatacg ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt
      121 tatatatttt gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc
      181 ttcagcaaca gaaaaaaatc taaactataa aatttagggt tgactaaaat ataatttttg
      241 tataccatat gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga
      301 aaaagcttaa tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac
      361 aagaaggtgc tttaacaggt attattggtc caaatggtgc tggaaaatcc acattgctta
      421 aagcaatgtt aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca
      481 ataaaaaact ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc
      541 caataaaagt aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa
      601 gaattaaaaa tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa
      661 aatattctaa tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag
      721 caagatgtct tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag
      781 acttagttag tgaacgaata attatggata cgttaaaaaa attgaaacat cagggaaaaa
      841 cagtgctaat tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc
      901 ttttgaatcg aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagaaaatt
      961 taaaaaaagc ctatggagat actatattta ttggagaggg ggaataatat gcttcaaaat
     1021 tttattaatg gtctgtatga ctttcacttt ttacagaatg ctctaattac atctgtagtt
     1081 atagggattg tttctggaac agtaggttgt tttattattt taagaggaat gtctcttatg
     1141 ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa tttcttatat tttaggtatt
     1201 aattttttta ttggtgcgat tgtatttggt ctattagctt ctatcattat tacttttata
     1261 aaaagcaata gtgtaataaa aggagataca gcaattggta ttactttcag ttctttttta
     1321 gcactaggag ttatccttat aggagttgca aatagctcaa cggatttatt tcatatttta
     1381 tttggaaata ttttagctgt acaagatatt gataagtgga ttacaattgg cgtttcggtt
     1441 ttagtgttgg ttatagtagt tctatttttt aaagaattat taatcacttc ttttgatcca
     1501 ttaatggcaa aagcaatagg gatgaatgtc agtttttatc attatctatt gatggttcta
     1561 ttaactttag tagcagtaac agctatgcaa agtgtgggaa ctatattaat tgttgcaatg
     1621 ttaattacac cagcagctac agcatattta tatgcaaata gcttgaaaac tatgatattt
     1681 atttctgcaa cagtcggagc aagttcatca ttgcttggat tgtttattgg ttatagtttt
     1741 aacatagcag caggatcaag tattgtttta acagcggcat tcttatttgt aattagtttt
     1801 ctaatctcac caaaacaaaa atttttaaga aagaaggaga gatcgttatg atgagaaaat
     1861 ggaaagcagt actgggaagt ctggggattt tgattgctct ttttatattc ggggcatgtt
     1921 caataaatag taaagacaaa gataaagtgg cttcgaacga aaaattaaaa gtagtagtta
     1981 ctaattcgat tttagcagat attaccgaaa atatagcaaa agataaaatt gatttacaca
     2041 gtatcgtacc tattgggaaa gatccccacg aatatgaacc tttgcctgaa gatgttcaaa
     2101 aaacttcaaa agcagatttg attttttata acggtgttaa cttggaaact ggaggaaatg
     2161 cttggtttac aaaattagtt aaaaatgcga acaaagagga aaacaaa
//
LOCUS       DASJTM010000203         2200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJTM010000203 DASJTM010000000
VERSION     DASJTM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.207"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(165..1091)
                     /gene="cysK"
                     /locus_tag="U4B29_003006"
     CDS             complement(165..1091)
                     /gene="cysK"
                     /locus_tag="U4B29_003006"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN2107885.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1231..2118)
                     /locus_tag="U4B29_003007"
     CDS             complement(1231..2118)
                     /locus_tag="U4B29_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2107886.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          616 a          486 c          365 g          733 t
ORIGIN      
        1 agaaaattca attttattgt attttaatga aaaaagacgt aaaacagtcg cgttcactat
       61 tctacgtctc tattcaaata atttttctca tcataaattt ttgtgtgttc ttttcatctt
      121 taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa
      181 agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct
      241 tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct
      301 actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta
      361 atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg
      421 attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt
      481 tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg
      541 atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct
      601 tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat
      661 tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca
      721 gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa
      781 cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact
      841 cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct
      901 ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt
      961 acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt
     1021 acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag
     1081 atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct
     1141 gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata
     1201 tatggtaatt tctttgcctt tttatctaaa tcaaacgttt agttcttgac caactaataa
     1261 agaataactg gacatagaca tgccattttt cgccattaga tcatcaacag acatgccaaa
     1321 tttttgtgca atatcccata gcgtatcacc tgacacgact gtatatgtct gaccattatt
     1381 tgtatttaca tctgttgttg aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt
     1441 tgtacttgtg gttgttgtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata
     1501 agtattaatc aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt
     1561 caagtacgct gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg
     1621 ataatcgccc gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc
     1681 ccaataagaa gaatattttc ggaatggctc attcatcgtt acccattgtc cgtttacata
     1741 ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt
     1801 ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc
     1861 gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat
     1921 ttgatcgata aacgcttgag gatcagcgac tgtttgagat ccttgttcat ctgcatgaac
     1981 atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc caattaaagg
     2041 agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgcc gggcactgcg
     2101 ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct
     2161 cttcttaaaa caaacttaat taattaaatt atacttttaa
//
LOCUS       DASJTM010000204         2156 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJTM010000204 DASJTM010000000
VERSION     DASJTM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.208"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            626..1795
                     /locus_tag="U4B29_003008"
     CDS             626..1795
                     /locus_tag="U4B29_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2107887.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          812 a          290 c          327 g          727 t
ORIGIN      
        1 actagtatga tatatatcta tagcaaacag ttaaaatctt cctaaaataa gcgaatagca
       61 ttcctaaaac ctataaaatt tttagatgaa gcgacaagtg gacaattaat aaactacttg
      121 aacatgaaat tatggatcac ctttcaaatt tagaaaatgg aattcttata cttattgcgc
      181 aacatttatt gattgcaaaa ttatgtgaca aaaaaaacta gtagaagaaa gaataatggc
      241 ttgataataa agtactacct attaaaggta gtactttatt taatttagta taaattaaca
      301 tactactata ttttaggttt catattatat aaattaataa tatactacca tttgttggta
      361 gtataattta aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga
      421 aagtttaata agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat
      481 ctttagtgat ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta
      541 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
      601 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
      661 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
      721 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
      781 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
      841 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
      901 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
      961 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
     1021 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
     1081 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
     1141 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
     1201 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
     1261 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
     1321 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
     1381 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
     1441 tttcaaatta gtttatcaag caagttcaaa agaagaagca aataatcaaa taaggtttat
     1501 gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga atcctgctat
     1561 attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa ctaatttgat
     1621 tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat ttcctaatga
     1681 agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa agtttctatg
     1741 ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt tttaacttga
     1801 tcaacagaat gggcttttcc atttacacaa aattattgac gctcccgaca aaattatgat
     1861 cttgcgtaaa gttataaaat attaataaaa atattttatt aatgaaaagc ttggtagcac
     1921 ggttattcat atgctaattt atttcacttt atagtaaggg gagttcttag tatattgttc
     1981 aaacttaaat ttcatataat ttaattatgt aaattagcta ttctaagaac ttctacggat
     2041 aactgcccgt ctaaaattta aataagagct tagaatcttc atataagtaa ggttaatttt
     2101 tttgtacaaa aaagtcggaa tattctgagt tactgaaatg atataaaatc ataaac
//
LOCUS       DASJTM010000205         2147 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJTM010000205 DASJTM010000000
VERSION     DASJTM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.209"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            225..1625
                     /locus_tag="U4B29_003009"
     CDS             225..1625
                     /locus_tag="U4B29_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107888.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
     gene            1612..>2147
                     /locus_tag="U4B29_003010"
     CDS             1612..>2147
                     /locus_tag="U4B29_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107889.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLA"
BASE COUNT          722 a          316 c          357 g          752 t
ORIGIN      
        1 taaattttat gagaaatgct caagaaattt atacaacagg tgatttatct tttcatcttt
       61 cacgaatcaa agggttatct tctatttttg aagggccaat caactataca acatttaaca
      121 attatggaga tggactgaac tatttttatc cttttcttac aataattcct gcagtcgttt
      181 tttatggaat ttcaaataac ttaatattaa gttatgttct atatatttgg ttattaaata
      241 tttgtacgat tctgattagt ttttattatg gagaaaagtt ttttaaaaag atagatgctg
      301 cttttttatt tagttgttta tatacttttg caacttatcg aacagttgat atctattatc
      361 gatccgcaat tgcagaagca atcgctctta cgattatccc agctgtttta tattatgctt
      421 atggattgat attcaataga gaaaaaaaag tcatcgcttt atctgtcagt ataagtctac
      481 ttgtgtatac tcatgtctta actacactat tgtgtagtgc attgattgct tgtatgatta
      541 tagttcgttt gtttttaagc aagcaaataa aaaacgatat aaaatttgtg gtcgtgaatt
      601 tagtaaaggc tgtttttttg acttgtgcat taactagcta tttttggtat cctatgttgc
      661 agcagatgaa ttatcaagaa atacacagtc ccttcaaaac agatttgcag ctagaagcac
      721 tttctatttc agatagtatc attcaagcgt taaataacga tattacaaca tacactatgg
      781 ggttattagg attggttgcg ttgatttttc ctttattcat actgaaagaa atggagaaaa
      841 aagaaaaaat catctatgtt ggcgtagttt ctacatgggt atgcgtaact aatttatttc
      901 catggttttt gtttcaaaaa acaccgataa gcttactcca gtttccttgg cgtatcttag
      961 gtatccaaat ctttttttct tcaatgatca taacgcttgt ttttatgaaa aagactatga
     1021 ataaaaaata ttcatggtta atcattggcg ctactgtggt gtttctactc gtcacgtcta
     1081 tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa acacggtcat attgttatca
     1141 atgaagaaac actcccacgt tatacaacat cagatatggg aggattatac gattacgccc
     1201 caacagaagc gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa gtaggagaat
     1261 cgtgggaaaa agggaagtat aaagctgcag ataataacat tacttacaca gtggcaagca
     1321 acaaaaagca aaaagtgaca gtaccagtat atgcttattt aggtacacag gtaaaagtaa
     1381 acggtaaagt cgtaaatgac tcttataaaa aacatggctt agtgaatgtg gatgtaaatg
     1441 ctggagaaaa taagatagaa attacaacaa aatatactcc aactgctctc atggctttat
     1501 ggatcagtat tctgacttat tgtttagtgg tgtattcaag ttttaaacga tttgcaccta
     1561 ataagttgaa agattctcga tatgtgacta aaaaggagat aaacaagaag aatgaaaata
     1621 aataaaaaag tagtaatatt gagtggatta attatagtta gtagtatata ttctttcatc
     1681 tttttcaaaa atacacagag tatttatacc actggagatc tttcttttca cttatctcga
     1741 atcaaggggt tatcgactat ttttaccagt ccgattaatt atgagacatt caattatact
     1801 ggatatggtg taaattattt ttatcccttt ctgacttttt ttccagctgt aatgttatat
     1861 tggatgacaa aaaatttgat tgtaagctat attatttacg tttggttatt gaacttgtgt
     1921 acaacactcg tagcttatca ttatggcgag cggtttttaa aacaaaaaaa agcggcattt
     1981 ttatttagtt gtctctatat tttctctgct tatcggacag tagacatcta ctatcgttcc
     2041 gcaattgccg aagctatcgc aataacactt gttatccctg tattatttta tgcttatcaa
     2101 atcatctctg gaaaagaaga aaagtatccg tctgtaaagc tagcatt
//
LOCUS       DASJTM010000206         2119 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJTM010000206 DASJTM010000000
VERSION     DASJTM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.211"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..513
                     /locus_tag="U4B29_003011"
     CDS             <1..513
                     /locus_tag="U4B29_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2107890.1"
                     /translation="VKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAG
                     NIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPV
                     VNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDA
                     GAVGGTLYAT"
     gene            503..1243
                     /locus_tag="U4B29_003012"
     CDS             503..1243
                     /locus_tag="U4B29_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2107891.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1240..>2119
                     /locus_tag="U4B29_003013"
     CDS             1240..>2119
                     /locus_tag="U4B29_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2107892.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDS"
BASE COUNT          794 a          260 c          408 g          657 t
ORIGIN      
        1 gtaaaagaaa tctttaattc gttaagggaa gtcaatttat ttgaaattgg caagaacatc
       61 attgatggac ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga tactatcaaa
      121 ggtattgcag gtaatatcaa aaattggatt aaaggagctt tagatattca ttctccatca
      181 agatggatgc gtgatatggt tggtaaaaat attgttcaag gtattatcgt tggtattgat
      241 aaagagcaaa gtaagttaga tcaaaccatg acaaatttag taaaaacacc gtctgttcaa
      301 cctgtagtaa atggaataag tcacctacca attacacaaa caaagcaaaa tatatctgct
      361 agtgaaacaa aagaaattca tttacattta aatgtttatg gagatttacc tgattcaatg
      421 gttcggaaga ttaccacaaa aattaaaaca gaattgacaa gacaaatgaa acgagatgct
      481 ggtgcagtag gaggaacatt atatgcaact taagagagga caatttttta ttaatcaaca
      541 ttattcttct gaatttaatg tgtatattca aaacaggcct gcttctgttt cagctagtcg
      601 agtaattgaa ttgagagagc gagaaggaaa tgattctatc attattgata aaggctatta
      661 caaaaatgtg actagaaaga ttgagtgtta ctataaagca ccttcaattg atatggtgca
      721 ggagtgggaa gatcgaatca ctgaatggtt agatatgtcg tcttatagtg attttattct
      781 ttactatgat gaacaatata tttatcaagc tgtagtaata gaaggacctg aatttaaagg
      841 aacaagaaaa acaggaaata ttgtcccttt tgaatttaca gtgagtattc gaccatttaa
      901 ggaaaattac aatggtagat ttactattcg acaaacagaa acttttgaaa tttataaccc
      961 agagaagtat tcttcaaaac cgcttattaa attgagtggt tctggagatg cttcttttta
     1021 tattaataag gataaatatg atttgaaatc gttggacaga gaattattta tagattctaa
     1081 attagaagag gcttatcgaa aattagatgg taatttagaa catcaggatc aggtcacctt
     1141 atttttagat tttccatttt tatatcctgg aaaaaatgaa attaaatgga cgaaaaatat
     1201 tcattcattt gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag aatatatagt
     1261 cctactgaaa cagatttctc aacgaatggc ttaggaattt taaaggattg tacaagatgt
     1321 ggaatatatg aagtagctaa tggaaaatat gaattagagt tggattatcc tttaggaact
     1381 agatttgatg aatattttga aaatgactat caaataaaag caaagccaaa cgatcaagaa
     1441 gagtatcata tcttttttat tgatgataaa gatatagata cttttttaaa tacagtaact
     1501 atttatgctc agagtcgtac aaatcgactt ggaagacggg tagtcactct tgtggaagtg
     1561 gactctaaaa ctggtcaaga agcgatatca attattgaaa ctaaaatgga taaaaaatct
     1621 gacatacgac tttattctga tattacggct gtttctagca caatctttga agcgagaaat
     1681 gttttaaatt gtattgctgg tgaacaagga tcattgcttc aatattgggg cggagaaatt
     1741 aaacgtgaac catttaagct ttctttgtta aagcgaagag gtcgcaataa tgttggaacg
     1801 attcggtatg gaaaagatat gtctggtttg aaggtcaaat tagattggac aggtataaaa
     1861 acaagaatta ttccatatgc tgatcctcaa agtgaagtag gtacgactaa tcgaatttat
     1921 ggttcgccag tagatagtgc ctatattaat aactatcctg atgtctatac agaacatgtt
     1981 cagtttacag aagaacaagg agtaaaagac attaagagct taaataaaat agctaaaaat
     2041 tactttaaaa cgatcaatcc tggctgtgat aagcctaaag tttctattac ggttgaattt
     2101 gataagttga ctgatagtg
//
LOCUS       DASJTM010000207         2111 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJTM010000207 DASJTM010000000
VERSION     DASJTM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.212"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..>2111
                     /gene="acm"
                     /locus_tag="U4B29_003014"
     CDS             16..>2111
                     /gene="acm"
                     /locus_tag="U4B29_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN2107893.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFA"
BASE COUNT          834 a          342 c          412 g          523 t
ORIGIN      
        1 ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt
       61 actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc
      121 agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa
      181 gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc
      241 aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg
      301 attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt
      361 aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc
      421 agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat
      481 aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat
      541 tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt
      601 aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga
      661 caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt
      721 tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat
      781 ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg
      841 aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat
      901 cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat
      961 catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg
     1021 aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta
     1081 agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa
     1141 cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa
     1201 gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac
     1261 gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc
     1321 acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta
     1381 ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac
     1441 aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa
     1501 tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat
     1561 caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca
     1621 ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca
     1681 gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat
     1741 tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact
     1801 gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac
     1861 agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa
     1921 aattttccag agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa
     1981 atatttgctc atacaggaga atccagcgaa caaagaagca attatattag taagagagct
     2041 gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa
     2101 tctagttttg c
//
LOCUS       DASJTM010000208         2075 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJTM010000208 DASJTM010000000
VERSION     DASJTM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.213"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..239
                     /locus_tag="U4B29_003015"
     CDS             <1..239
                     /locus_tag="U4B29_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2107894.1"
                     /translation="YIKEKPDLVICTGVLAMIPICLITKMMGKKLIYIESFAKVTSPT
                     QTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            256..735
                     /locus_tag="U4B29_003016"
     CDS             256..735
                     /locus_tag="U4B29_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HEN2107895.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            755..1822
                     /locus_tag="U4B29_003017"
     CDS             755..1822
                     /locus_tag="U4B29_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2107896.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
BASE COUNT          779 a          224 c          416 g          656 t
ORIGIN      
        1 tatatataaa ggaaaaaccg gatctcgtga tttgtacagg tgttttagct atgatcccta
       61 tctgcttaat aacaaaaatg atgggaaaaa aactgattta tattgaatcg tttgcaaaag
      121 taaccagccc tactcagaca ggaaaattga tgtatcattt tgcagatcaa ttttatgttc
      181 agtgggaaac tatgctacaa ttttatccta atgctattta tttgggtggg atatattaaa
      241 taataggaga aaaaaatgat atttataaca ttaggttcac aaaaatttca atttgataga
      301 ttgttaaaag ctattgatga tttaattgaa aaaggagtaa taatcgaaag tacatttgca
      361 cagagtggtt atagtgatta taaaccacta aattattcct ataaagcatt tctaaatagg
      421 gatgagttta ctgaaactat gcaaaaagca gaaatggtga ttactcatgg aggtacaggt
      481 gctataattg gggccgtaaa aaaaggtaaa agggtcattg ctgttcctcg attggctaag
      541 tatggtgagc atgtcgatga tcaccaactg cagttaatta gacaatttga tgagatgaat
      601 attatctatc cttgttatga attagaaaat ttagaagatg ccgttaaaga aacaagagtt
      661 cggatgttta acaagtatgt atctaacacg gaaacgatta ttaattcgat agaggtatta
      721 atagaaaaaa tataaatata aggagtaaag cgttatgcaa agagtactta tgattggtaa
      781 tgacagctcg gtaaaaggtg gaattacaag tataattaca caaatgctca attttaattg
      841 ggaaaaaaag aatatcgaga tgacttttat accgagttat aagggtggaa atgcaatatt
      901 taaatcttta tattttatgt atgcatttgc taaaattata ggtatctctt tattgagtaa
      961 acctaatatt attcatatgc acatgtcgta cagagggagt tttagtagga aatatgtgct
     1021 gaataagtgg tttagattat taaatattcc tgtaatcatc catcttcatg gatcggaatt
     1081 taaaaaatgg tatgatagtg tttctgtaaa aaaacaaaat cagattcgct tgctgttaag
     1141 agagtctgat tccacgattg tacttggaga aaaatggaag aagataatta aagaaattga
     1201 gccaaattgt aaaactgttg ttgtagaaaa ttctattgta gttccagaat ataccataaa
     1261 gtggaattca aaaatgtgcc gatttctgtt tatgggagtt ttgataaagc gaaaaggtgt
     1321 acacgatttg gtagaagcag ttaaaaacat tgcagaacaa tatccagaaa aaaagtttat
     1381 ttttgagatt gctggagcag gtgatgaaga aatatcgctc aaagaattga tagaagaata
     1441 taatttgcaa tcgtattttg aatttacagg ttgggttgtg ggagaaaaaa aggaggagtg
     1501 ctatagaaga tcaaatgtga tgattttacc gtcctataac gagggattgc ctatatctat
     1561 tctagaggcg atttcttatg gaatgcctgt aattgctaca gatgtaggag atatttcttc
     1621 agcagtacgt gatggagaaa atggatattt gtttcaacct ggtgacgtat cagcgttgac
     1681 atctgcaata ctaaaaatca atgataaaga taaatacgta aaattttcac gttgttcgaa
     1741 ggatttggca ataacgaaat ttaacgatgg aaatttcttt gatagaattt taaatctgta
     1801 tcaaaaaggg agcaaggagt gatgacaagg aaaataaaaa atgtttcgat tatgtaatga
     1861 acttattaag tgaataaaat agagagaaaa aatattgaaa tagacgatgt aaatttttat
     1921 ccgtgtaaga attcaaatga atagtagtgt tttagtgctt gtaatagagc aagagtacaa
     1981 gcacggtaca ttatataaaa acattaatga tatttctata aataaataga ttttgtattc
     2041 tggatgatga agaggaagat attatctgat aactt
//
LOCUS       DASJTM010000209         2019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJTM010000209 DASJTM010000000
VERSION     DASJTM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.214"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="U4B29_003018"
     CDS             100..570
                     /locus_tag="U4B29_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN2107897.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="U4B29_003019"
     CDS             792..1895
                     /locus_tag="U4B29_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2107898.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DASJTM010000210         2003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJTM010000210 DASJTM010000000
VERSION     DASJTM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.215"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U4B29_003020"
     CDS             <1..378
                     /locus_tag="U4B29_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN2107899.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U4B29_003021"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U4B29_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2107900.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U4B29_003022"
     CDS             1859..>2003
                     /locus_tag="U4B29_003022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107901.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASJTM010000211         1983 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJTM010000211 DASJTM010000000
VERSION     DASJTM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.216"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..735)
                     /locus_tag="U4B29_003023"
     CDS             complement(34..735)
                     /locus_tag="U4B29_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2107902.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="U4B29_003024"
     CDS             complement(751..1191)
                     /locus_tag="U4B29_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2107903.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="U4B29_003025"
     CDS             complement(1191..1766)
                     /locus_tag="U4B29_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN2107904.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          573 a          415 c          306 g          689 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     1861 ggatagacgc cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat
     1921 aaaacaaaaa taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact
     1981 ggg
//
LOCUS       DASJTM010000212         1979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJTM010000212 DASJTM010000000
VERSION     DASJTM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.217"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="U4B29_003026"
     CDS             complement(325..528)
                     /locus_tag="U4B29_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107905.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="U4B29_003027"
     CDS             complement(728..1744)
                     /locus_tag="U4B29_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2107906.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DASJTM010000213         1935 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJTM010000213 DASJTM010000000
VERSION     DASJTM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.218"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..586)
                     /locus_tag="U4B29_003028"
     CDS             complement(<1..586)
                     /locus_tag="U4B29_003028"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN2107907.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDF"
     gene            complement(670..>1935)
                     /gene="uxaC"
                     /locus_tag="U4B29_003029"
     CDS             complement(670..>1935)
                     /gene="uxaC"
                     /locus_tag="U4B29_003029"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2107908.1"
                     /translation="DKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAP
                     GNPIFEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAIC
                     TTDDPTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKD
                     FNGLKQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSE
                     IAQFVTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQ
                     NVVALFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTA
                     EGMRQQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDD
                     EMYLSQIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT          594 a          424 c          275 g          642 t
ORIGIN      
        1 caaaatcacc tttttcaaca gaagccaata aattcccacc tacaccaact gcaacaactc
       61 ctacttcaaa ccaatcttct aagttctcta tacttactcc gccagtaggc attatattta
      121 cgtgtggtaa tggagcttga taggctttta caatggatgg accaaaagca gaacctggga
      181 atagtttaat gatatctacg ccagcagtaa gtgcctcctt tatctcagta attgtcatac
      241 aacctggtaa ataaggaatt tgatataaat tacaaatctc agctgtttgt cgatcaaaag
      301 atggactaac gatatattct gccccagcca taattgcgat acgagcagta aacgcatcta
      361 aaaccgtacc ggctccgatt attacatctt tttgatcagc atatttttta gcaatttctt
      421 tgattatttc ttcagcatta ggaactgtaa aagttagttc aatcccttta attccacccg
      481 caataattga atttattgct ttaatggcct catccttatt tttcccacgt acaaccgcaa
      541 tgactccagc cgcttctaac ttacttaaaa tttctacttt tttcatatct atctccttcc
      601 tttatggata catactttgt ctagctgtac taacttacaa agaataaatt taagctttta
      661 tttcaagtat tattttctac ttccgatttc aaaaaaatta aaataattat acgcattacg
      721 ataacaaata ttctccacta tctgactaag atacatttca tcatctggaa ttcggcctgc
      781 tttaacccac tcgcttagta attgacataa tactcgacga aaatactcat ggcgaggata
      841 gcttagaaaa cttcgagaat cagtcaacat acctgtaaaa ttcgacaaca aactttgttg
      901 tgccatcgta gtcaactgtt gacgcattcc ttcagctgta tcattgaacc accaagcaca
      961 tcccaattgg attttttgaa tcataccccc ttggaagctt tgcattgctg ttgctagtgg
     1021 aagccaatca gttggattta atgaatataa aattgttttt ggtaagcacc cactcttttc
     1081 agcggtatcg aacaatgcta caacattttg tgtcaaacta gcttgatccc ccatcgaatc
     1141 accaccgaca tctactccaa tttgttcctt taatatactt gaattatttc ttaaagcatt
     1201 taaatgcatt tgcatcgtcc aaccatatct tgtatactca gacatcaaat gtaattgaat
     1261 acctgtaaca aattgtgcaa tttctgataa atggaaatcc gtttttcctg ctaaagcttt
     1321 tattaacaga ttgtcgagta ttttttcatc tgctatttca taaaaataag tattcaaacc
     1381 gtgatctgct aatcgacagc caatttgatg aaaatgatcc actcgttggc tcaatgcttg
     1441 cttcaaccca ttaaagtctt tgattggtat agccgctgtt tgactaagtt ttaaaatata
     1501 gttcgaaaag cctgatgatt tgatactcca gaccttatct ggacggaaag taggtaatac
     1561 cttgaaagaa ttatcctgtt tagaaagctc agtatgatac cacaagtcat ctgtaggatc
     1621 atccgttgta cagatggctt tcacatccat catctccaac aattctttcg ggcgatagga
     1681 atccgtttga attttttcgt tagcacgtcg ccaaatttct ggtgcattct tctcagaaat
     1741 tgtcaaatca atatcaaagt aacgttgtaa ttccaaatga gaccattcaa aaataggatt
     1801 acctggcgcc ttctccactg tttttacaaa agctaaaaat ttttcataat catcacctgg
     1861 accactgatc aactcttctt ctataccatt agctcgcatc aatcgccatt tataatgatc
     1921 tccagctcct ttatc
//
LOCUS       DASJTM010000214         1921 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJTM010000214 DASJTM010000000
VERSION     DASJTM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.219"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..424)
                     /locus_tag="U4B29_003030"
     CDS             complement(<1..424)
                     /locus_tag="U4B29_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2107909.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="U4B29_003031"
     CDS             complement(533..1564)
                     /locus_tag="U4B29_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2107910.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          590 a          321 c          332 g          678 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 a
//
LOCUS       DASJTM010000215         1905 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJTM010000215 DASJTM010000000
VERSION     DASJTM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.220"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U4B29_003032"
     CDS             complement(<1..914)
                     /locus_tag="U4B29_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN2107911.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U4B29_003033"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U4B29_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN2107912.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          631 a          351 c          370 g          553 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataa
//
LOCUS       DASJTM010000216         1816 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJTM010000216 DASJTM010000000
VERSION     DASJTM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.222"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..1184
                     /locus_tag="U4B29_003034"
     CDS             15..1184
                     /locus_tag="U4B29_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2107913.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1509..1757
                     /locus_tag="U4B29_003035"
     CDS             1509..1757
                     /locus_tag="U4B29_003035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107914.1"
                     /translation="MIIEINNEILEKNVYEYLIVTLFIPLSLFTVTKGPPCFSTVAFF
                     ELTVTVNPLYRNTGKTIVLPFEDLNVNVISSLFGEKLL"
BASE COUNT          642 a          312 c          283 g          579 t
ORIGIN      
        1 ctaaataaac tattatgaaa aaaggaaaac atgctaaaaa aagaaaatat tcatttttaa
       61 tgatcgttgt cgtgttgttt agccttagtg cgttctctac aggaactatt cttggatggc
      121 aattttacaa taatgaacac tctcagatag ttgcccatgc tcaatcagct caaaaaaagt
      181 tgatactgac taccaatgaa caacagctcg ttgaaaaaat cgatcaacta ttaagaaaaa
      241 atgattatat tggctcaatt tacgtaaaga aaaataatcg catcatcatg gaaaaaggct
      301 atggctatgc gaataaactc acgcaacaac caaatgatcc ttctctttat tatcagattg
      361 gttcggttca aaaagcattg actactcttt tgattttaaa acaagtcgag cgtgggcacc
      421 tttctttaga tactaagctg gctcaattct atccacaaat tgctggcagc cagcaaatta
      481 ctatccgtga cttgctgtat atgcgctcag gcttgcaccg aactgcttct ccaaccatac
      541 cgatgactga tgaagaagtc gttcaatttg cgattcagca cttaaaattt acagattatc
      601 agacctctca ttatgattca ttgaacttta ccatattgac tggaattttg atcaaattaa
      661 caaatcaacc gtatgaaaaa ttattgaccg atgatatcat cgaaccttta ggattacagc
      721 atacaaaatt ttatgaccaa gtcaaaaatt catccaatca tgcactatcc tatcaaatgt
      781 ctaaagagga tgattacttt aaaccactga cagaatctga aacagagatc cataacgaat
      841 tagggactgg taatatcagt atgtccgtct atgatttaaa tacgctgttt acacaagttt
      901 tatctggaaa actagtttcc aaagacttac tcttctcatt atggcgtgaa aatgcgaatg
      961 gacatccata tagtggtggg gtatatagcg gagataatta tatcctgtcg caaggaaata
     1021 tcgactcttt tgatacatct gctatattta gcaaagattc acaggatgct atcatcatgg
     1081 aaagcaatat aaaagctgac aaaactatca aactgcctac tgctgatcta agaaaccaaa
     1141 tctatgacct gatggaagga actactaagt tggcaaatag ctgatatgat gaaaagatag
     1201 caaatactac ttctttgaat agcatttgct taaaaaaagt actcgactaa acgtaggaaa
     1261 gagtgtggga caaatctaaa aatcaatttt tgctacagac tttaattcca aataaacaga
     1321 acagaaagag gtgttcttct ttctgttctg tccaaattat tctaagattt ctctttacca
     1381 acattatttg acaaagggcc tattaaatct aaaatagtat ctttagattg ctaaaatatg
     1441 tgctagcaaa tcattcatgg cttgttctta tttttaattt tatgcaataa aaaagaagaa
     1501 caattagcgt gattatagaa ataaataatg agattttaga aaaaaatgtg tatgaatacc
     1561 tgattgttac tttatttatt ccactttcac tttttactgt aacaaaagga cctccttgtt
     1621 ttagtactgt agcatttttt gaattaacag taaccgttaa tcctttatac cggaatacag
     1681 gtaaaactat tgttttgcct tttgaagatt taaacgtaaa tgtaatttca tctttatttg
     1741 gagaaaagct actataaata acttgaccat cttctgtaac ttgatggttt ttcaaaagca
     1801 attgatcttt tttata
//
LOCUS       DASJTM010000217         1811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJTM010000217 DASJTM010000000
VERSION     DASJTM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.223"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(185..1204)
                     /locus_tag="U4B29_003036"
     CDS             complement(185..1204)
                     /locus_tag="U4B29_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2107915.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1201..1425)
                     /locus_tag="U4B29_003037"
     CDS             complement(1201..1425)
                     /locus_tag="U4B29_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2107916.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1422..1715)
                     /locus_tag="U4B29_003038"
     CDS             complement(1422..1715)
                     /locus_tag="U4B29_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107917.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          513 a          371 c          308 g          619 t
ORIGIN      
        1 tgttgccaca taaagccata aacaatcaac cagtagaatg tgtggcaaca aacctgttat
       61 cgcatagctt ggagtgtgac tattatgggt gatagtgaag atatgcgata acatcactat
      121 tttatcgaat gatttttata gttgtcaata tagttatgta atgctcctca acgaggagct
      181 atttttatcg tttaggaata tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc
      241 ctttagtgtt tccctctgga tcattcgttg cccgcatcat gacatagact ttcttattag
      301 ggaagttacg catgttaaaa gatacatgat agccaacatt tccagaagta ttataagctt
      361 gatttacatc tgatctataa attccatcag ctcttactcg agctaattct ttcccagtat
      421 tgtaatccat aatgaagata tactcgtatt tatagttagc aatgtgccat ccagctacat
      481 gcaaatttgc attttcgatt tctccaaact gatcaatgtg ggcgtaattc gttccatctg
      541 tcagtgtagg attagctgca ccagctcgtg ttggatcaat gacaggcttg ttttcagaag
      601 ttgttggatt ttcatcggta aatccatgag ctaaatcata tgctaatttt tctttactta
      661 cgcccatttc agaaagataa ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta
      721 cccataaatg cgatttgatt cccggttggt tataaggagt gtccaacgtt aatggaatac
      781 catatttcat tgctgaatct ctagccaatt caacgtatgc cttgtagttt ttctcaaacg
      841 ttgctttatc atgtgtgtgt tgtaactcaa tctgcacagg actgttggca ttagcatacg
      901 aaccagcacc gtactgtaca taaccaggtt gaccgacttg ataaacaatt ccgccgtctc
      961 ccacaatata agcagtataa gcgctagtcc atgaacgttg catatactgc gcttcattgc
     1021 gtcctgttgc tgtttcatta gccgtttcat gcagtaaaat atactgatta tttgctactt
     1081 gtgagctacc ttcatttgcg cccaaattaa attcattgtt gatagtatag gcaaatccgt
     1141 taattggcaa taaaaaaaga gccgttaaca ggctcatcgc agtaatagta attttctttt
     1201 tcatttgttt cctccttctt cgctttcagc cgagaacatt ttgtaggttc gatttgatac
     1261 acccaacaca ctccctaaaa acgcgccaaa accagtaatg atgacaacac agatatctgt
     1321 gtactgccaa ttgagcgctt taccaactaa ccccacgaaa gtagctagtg cgggaataat
     1381 taccagtgcg aaccatttta gtacttcgaa cgttttatta ttcattttct tctctcccta
     1441 aacaaagttt tgatttgttg cgtgtgttcc accaattttt ctgtatgtgt atctaatctt
     1501 tcatcgtgtt tctttagttc ttcatgaatc atcaatcgat ctgatttgct cgattctaaa
     1561 tctttagtca gcaaatctaa attgtggctt acttttgaaa gagtctcagt aattttcgag
     1621 aaagatgcag caatcggtct aattactaat aaaatcaaag aaacgatagc ggttattgat
     1681 cctgctatca ttccccattc ccctaaatta atcatgtgac aactccttta ccttaaataa
     1741 aaacgcatca attaagatgc gctcttatct ttattaatga ttttatctgc ttcttcgtct
     1801 gtaatgcata a
//
LOCUS       DASJTM010000218         1809 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJTM010000218 DASJTM010000000
VERSION     DASJTM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.224"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            275..1753
                     /locus_tag="U4B29_003039"
     CDS             275..1753
                     /locus_tag="U4B29_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2107918.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          654 a          253 c          322 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttc
//
LOCUS       DASJTM010000219         1767 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJTM010000219 DASJTM010000000
VERSION     DASJTM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.225"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(223..1689)
                     /locus_tag="U4B29_003040"
     CDS             complement(223..1689)
                     /locus_tag="U4B29_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2107919.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          559 a          359 c          311 g          538 t
ORIGIN      
        1 acaagggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataaat ggtgttcaat
       61 cagctgacct tcagcattag ctcaaaaaac ggcaaaatat gaggagctat ttttgtttgg
      121 ctgttcttat tacttgatgc aggacagctt tttcacaacc tctgattcac ggtgttccag
      181 aagtagcttc tgtcacaacc tcttgtaaca agtatttcta ttttaaggct gaaccactgt
      241 ttctggttca gtaatttctt tgtttttgtc tcgacgagca attgctggca agatcatacc
      301 taataagatc aagacaattg gtgtgatgat attcgtgaat aattggaacc accatgcttt
      361 aggatcagca gtataatcta cttttggcac cattcctagg atacatgcaa acgcagtgaa
      421 ggcaaagcac caagcaccaa agataaaacc gatttttgga tttttaacaa atttatattc
      481 agaattgaat ttcttataag ctttattcaa catcatatat gccaagaaca cccataggta
      541 acgcattggc atgacaactg agttcaagtt cgtcaaccat tttactaatt cattcatatc
      601 tttgatacca aagatcggca acaaaataat gattcctact agtatacctg tcagaagata
      661 accattcacc aatgtacctt tacgattacg tttacgtagc cagtttggta caaattctgg
      721 atccgcatct gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta atgcggagat
      781 ttgtccaatc atgtttgtca atgcgtaaat gatcatcaat gagttaccga taccatagta
      841 attacctaaa atttgaaaag cactgtatgc cccatttgcc atcaagtctt tagggatatt
      901 atcgctagca aacagcatac ccattgcgac tgagcctaat acagcacaaa tccctaccat
      961 tgctgctaag aagatcatcc ctttaggaaa ttcctttgca ggatttcttg tagagttgac
     1021 ataaggggat attttttccg cccctccaac tgcaaatact agcatcgaaa cggttgtgaa
     1081 ataactgaaa tcaaatgttg gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa
     1141 ttccatcgtt ggattgatga acggtgccgt tacagctaaa agaataaata agatcgacat
     1201 gacgaacatc gctgttccgg ccagtccgcc aatcactttc aaagtagaaa gtccttttgt
     1261 tgataaatat aaaaaggcaa gaaatataat aagtgaaata actgctacca tttgtactga
     1321 catcgtattt acgatatttc catttccttg gactgcccaa ccaccagcaa tcagaattgc
     1381 ttgaggcttt tgtgccaaat aaggaatatg gacgacccag taagtccaag cggcatagta
     1441 tgccagtcgt ttattagatg tcgtattttc gacccatgaa ctgacgcctc ctttactatc
     1501 cttgaaggct gatcctaatt gtccaacgat caatgcgtat ggaataaaat aaatggccaa
     1561 aatcaatagc caagaggtga caactgatac tccttgctga gcatagttat taactacatt
     1621 ccccatgccc caaaccatgt taaatgcaat taacgcaaca gtaaaccagc gtaattgttt
     1681 gttgttcatt cgttctctcc tttatgataa aaatttgtta tccagatgta atcatattca
     1741 atttttaaca aacttacaag acagtgt
//
LOCUS       DASJTM010000220         1702 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJTM010000220 DASJTM010000000
VERSION     DASJTM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.227"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..737
                     /locus_tag="U4B29_003041"
     CDS             141..737
                     /locus_tag="U4B29_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2107920.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(795..1277)
                     /locus_tag="U4B29_003042"
     CDS             complement(795..1277)
                     /locus_tag="U4B29_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107921.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1383..1580)
                     /locus_tag="U4B29_003043"
     CDS             complement(1383..1580)
                     /locus_tag="U4B29_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107922.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          564 a          346 c          263 g          529 t
ORIGIN      
        1 tttttcttat taaaattgtt ttttgaactt cgcttatttt ttgtgagcat atctgtttac
       61 ttactttttg taaggtgcta tacttcattt cgtaatcaat taaaaaaatg caaaacgcaa
      121 atcaaaggag aaattttatt atgacaactt ttactgacac tttgaaaaac cgccgttcaa
      181 tctatcatct aggacgtaat gtttcacttt ctaatgaaga attaacaact ttgatcaaag
      241 aagcaattaa agaaagccca actgctttca atgctcaatc aacacgcgca gttatccttt
      301 tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga agcacttcgc ccattgactc
      361 cagcagaagc tttcccaaat acacaaaaca aattagctgg tttcaaaaat ggttatggta
      421 cagtattatt cttcaaagat actgacgtgg taaaaggtct acaagaacaa tttgaattat
      481 atgcagacaa cttcccagat tggtcagaac aatcaaacgg aatcgcaaca gctaatactt
      541 gggtagcatt agttgataaa ggattgggcg caaacttgca acattacaac ccagttattg
      601 acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg gaaactacgt tcgcaattag
      661 tattcggttc accagaaaca ccggctgagg aaaaagaata catgaacgat gccgatcgct
      721 tccgcgtatt tggataattt tatttgaaat aaaaaacaga ggttgtgaca aacatcttgt
      781 cgcaacctct gtttttattc tggatcaatc ataatatcaa tctctgcctc atcgtttaag
      841 cgttgggaca aaaatggaac taattcttct aatgatgcag aaaatttcag catcggtcct
      901 cgccatccgt ttcggaaaaa attcattttt acgattttag aaccttttgg ctgatataat
      961 acaattttat tgatctcaga aaaaggaaca ccttttttgt caaagctatt caaaacaagt
     1021 ccttcttctg tgattcctct actgtccaac aaaaaactaa taaaaatcat tgctgacaag
     1081 atcccttttg ccaattcatc tgctgtagga tgactgatga gtgtatacca aatgactagt
     1141 gacactaaga agggtatcac caagtagacc catcgatttc gcacagtccg ataaataatt
     1201 tcttttcgaa ccagccaaag aaataaaaac atcacaacaa cgaataaaaa caatagaacc
     1261 gtaaagaatg ttaccatgac aactcgcctc gctttttctt ctccagaaaa taggaaaaga
     1321 atactgattt accttctcaa agaaattgga cttgcagcac gccgcacgtc caattgtttt
     1381 ctttattcaa atgtatcata ttttgcaaca accatgcttg ctttttcatc taataattct
     1441 tggtcctctg cagcacattg ggcaactttg accacggcgc agtttgtcat ttttgataca
     1501 acttcaccaa tgaccgtttt tgtaaaacca attggtttgc atgcatattg ttctcctaaa
     1561 ttaatcattt cttccgtcat caaaatcctc ctaaactgct ggaacttttc tccacattac
     1621 cactgctatt tctttggaga tttaactcat tgatttttat ttacagttaa aaatcaacct
     1681 tacaggcatt atagcgtact gt
//
LOCUS       DASJTM010000221         1692 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJTM010000221 DASJTM010000000
VERSION     DASJTM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.228"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(361..>1692)
                     /gene="brnQ"
                     /locus_tag="U4B29_003044"
     CDS             complement(361..>1692)
                     /gene="brnQ"
                     /locus_tag="U4B29_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2107923.1"
                     /translation="KDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVT
                     GIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTSFE
                     IGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLL
                     LAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTK
                     PAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLGTY
                     GSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTN
                     IIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPET
                     IKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT          597 a          341 c          299 g          455 t
ORIGIN      
        1 tttttttgcg aaaactattt ttatatcata tatatgtcta aaatctatat tataaaagaa
       61 atgttgtttg atcagcttct cttcggtatt agttcaaaac aaaacggcaa agtatatgga
      121 gctactttgc cgttttgttt ctctaatact caggtcagtt tatagacgct caataaacgg
      181 tgttcaaaag ccagtttctc ggtattaagt caaatttatg caaaaatgtg agaagccatt
      241 ttcaaaaaat atttcttaat actcgaaact gagcggcttt ttcacgacct ctcaataaac
      301 agtgttcaag agacagccta tcggcaataa ggctgaacgt ctctttcaca acctctttta
      361 tcaatctgct acttcttctt tcttagcgaa actccaaatc agtccgataa caaaaccgat
      421 aactgctggt acgatccacc ccatccccaa tttgaaaaac ggcaagtagc tttccgcaac
      481 atataaaaga ttttgaacaa taggtgtttg tttgatagtt tctgggcaag cgttcaaccc
      541 atcaaaaatc gaagcaatca atgtaaaata agtagtcatt cgataaacag ccgtacgctg
      601 cttaaacaaa ggaccaacta acactagtaa aatcagtgtc attgctaatg gatagatgaa
      661 catcaaaaca ggcaacgaaa attgaatgat attcgtcaag cctacgtttg ccgctaaaca
      721 aggaaggata ctagcaagtg caacaaagaa tgcgtaattt ctttgcggaa acaaatctgt
      781 gaatgtttct gcaaaagctg taatcaaacc aataccggtc ttcaagcaag ctaatataac
      841 gataaatgct aataagatac tgccataagt acctaaataa tgatcagcaa tctgagccag
      901 cgtgatcccg ccattttcac ttagagcaaa gctacctaag ctcatagcac ctaaataagc
      961 gagcaaagta taaatgattc ccatcaatat aatgctgatc gcaccagatt tgatcgtgtc
     1021 tttagcgatt tcagcaggtt ttgttacacc catgttttgg attgttgtga caatgatgat
     1081 cccaaatgct aaagcagcaa gcgcatctaa tgtgttatat ccttgagtaa accctgtgaa
     1141 aaaagcatgc tcttgataat ttggctgaat aggcgcttga tttacagaac ctagtggatt
     1201 caaaaaagca aggagaagca aagcacctaa taaaaccaaa aatgcaggat tcaggaactt
     1261 tccaacataa tctaatatct ttgtcggttt tcttgaaaac caccaagcca gcacaaaaaa
     1321 tacaatactg aaaattgcta gatatagcgt ctgtctgcta ggatcgataa atggtgctaa
     1381 accaatctcg aaagatgtgg ttgcaaggcg aggtaaagca aaaaacggtc cgataactaa
     1441 gtaaagcaag atggtaaaaa ccatcgcata tgtttgattt atccgttttg ctaattcata
     1501 tacacctgag cttttcgata caccaatggc aatcacccct aagaagggaa gaccgatacc
     1561 ggtaaccaag aaacctagat tcgcccaaaa ggtagcagcg cctgcttctt gacccaaatg
     1621 aacgggaaag ataagatttc cagcaccaaa aaatagaccg aaaagcatgg agcctataaa
     1681 aagatagtct tt
//
LOCUS       DASJTM010000222         1637 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJTM010000222 DASJTM010000000
VERSION     DASJTM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.229"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1502)
                     /locus_tag="U4B29_003045"
     CDS             complement(183..1502)
                     /locus_tag="U4B29_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN2107924.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          526 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaatt
//
LOCUS       DASJTM010000223         1576 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJTM010000223 DASJTM010000000
VERSION     DASJTM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.231"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            356..946
                     /locus_tag="U4B29_003046"
     CDS             356..946
                     /locus_tag="U4B29_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEN2107925.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            973..>1576
                     /locus_tag="U4B29_003047"
     CDS             973..>1576
                     /locus_tag="U4B29_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2107926.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAAL"
BASE COUNT          555 a          173 c          310 g          538 t
ORIGIN      
        1 catatttaag cggaatgggt agaaatagga gattttaatc ataaaaaact gtactatagt
       61 gaatttttgg agatttatat tccagttata tattaatgat tgtgatactt gtactattgt
      121 aaaaattcgt taggtttaaa taactggaaa tatcggaaaa tgggcattat acatcacgga
      181 catcccttct tttggaaaaa atgttacgat ttttataagt aatattgtat ccggtggaat
      241 caacgatgaa gataacatat ccatcgttgt agattcagta gcattgaatg atgctcttgt
      301 tgcaagaaat ccgaataaaa ttgttaagta taaattgaat taataggaga tatgaatgga
      361 aattagcagt aaagaatatt taaaagcggt tcttcgtatg gaaaagaatc tatatataga
      421 atctaaagga caacacacaa aattactaaa aataatgaaa tcacatccgg actacaacgc
      481 ttggcggtat actcgtcgaa tgagaattac atcttattat tattcaaaga gaaagaaaaa
      541 tatcatttat gcgattatgt atattgtgtc ttgcagaaga atgaatagtt tgggaagaaa
      601 atgtggtata gagtgtggtg aaaatgtatt tgatgaaggt gtaagatttt accatacaca
      661 aggaattgtt attaatggta acgccataat aggaaaaaat tgttacttat atgggaataa
      721 ctgtattgga aatgatggaa tcgatcctaa gtgtcctgta attggaaata atgtacgtgt
      781 atgcgtgggt gcaaagatta ttggtaatgt aaaaatagct aacaatgtag ttgttgcggc
      841 aggagctatt gttatcaaag attgtctaga gaatggtgca attcttgcag gtgtaccggc
      901 aaaaataata gggtatgtta atttaacaaa agtattttta gaataatttt tagcgaaaga
      961 aggtgaataa cgatgcgtat ttcaataaaa agatggtcag gagcagattg gacattgttc
     1021 acgttggcat tttttgcaat ctccatagtt cttgtatata cttttaacgc cccaagagca
     1081 attttatatt tgggagatat aattaatatc tttgtgttat taaatgcact tagctttaga
     1141 cgcagaaaag gaatatatgc tagcaaaaat gagatgctga tagttttgtt tttactagtt
     1201 tctatttgta gctcagttat taatttagaa aatataggat tgattatctg ggggatacga
     1261 aataacactc gtatgttcat agtatatata aattgtgcaa catatttaac aattaaagat
     1321 tactataaag tattcgctat aattgaaaaa ttattctgga tttcactgcc actttgttta
     1381 attgaaagat tcatggtcac ctatccaact ggaacaattg taggagatat gataggtgga
     1441 gttttttgga attattcagg ttgtaatatg ccattgaatt taattttgtg tgtgacagtt
     1501 actgttgtta caatcggata tttcaaggga gaaaaaaatt tcatccattt tattttatgt
     1561 gtttgtgcag cacttt
//
LOCUS       DASJTM010000224         1550 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJTM010000224 DASJTM010000000
VERSION     DASJTM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.232"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..215
                     /locus_tag="U4B29_003048"
     CDS             <1..215
                     /locus_tag="U4B29_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107927.1"
                     /translation="SPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHY
                     NRLSEEKILLPENWAELRKLVEYGII"
     gene            202..>1550
                     /locus_tag="U4B29_003049"
     CDS             202..>1550
                     /locus_tag="U4B29_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN2107928.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     "
BASE COUNT          501 a          198 c          238 g          613 t
ORIGIN      
        1 tttcaccatc tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt
       61 taaatgaata tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt
      121 tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag
      181 aattaagaaa gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt
      241 aagtccagtt tctggtatac tatagggaat ttttttatta agggaatttc attcataact
      301 attcctattt ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact
      361 tcatttgtat caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat
      421 gcaagttttg actataaaga tgacataaat gaatttatgt catcggtact ttctctagct
      481 acactttcgt taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct
      541 ttttttgata tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt
      601 gtaattagtt atatccagtc ttattttacg attaacttta accattttaa atatttgatt
      661 ttatctttta tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta
      721 tattttgatg ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata
      781 ggtttagtaa tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag
      841 tggagatacg ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt
      901 ttagcacaat tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata
      961 tattcctaca ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac
     1021 gcctgggttc catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa
     1081 gttagtaact attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg
     1141 attgatttag caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt
     1201 ccgataacct taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt
     1261 tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata
     1321 ctattaaaca tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta
     1381 tttacgtatt ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa
     1441 caattatttg acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg
     1501 atacttcata ttttaatcaa tcaaatgttg attcgttacg ttttgacatc
//
LOCUS       DASJTM010000225         1510 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJTM010000225 DASJTM010000000
VERSION     DASJTM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.233"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..1147
                     /locus_tag="U4B29_003050"
     CDS             29..1147
                     /locus_tag="U4B29_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107929.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT          495 a          251 c          334 g          430 t
ORIGIN      
        1 gaatgatttt tttaggaggg gtacgtttat ggcaaaaaag gataaagaat tattgacaca
       61 attgacttca gaaggcgcta aaggaccatt cgtcacgatc atgctgaata cgcatgtcgc
      121 ccatcaagat gtggaaaaag atcagatcaa attcaaaaat tttgcgaaag aagcaaagaa
      181 acgttttgag aaaaaatatt ccgaacatga ctggtccccg ttccaagaaa aaattgaaaa
      241 tttgttagga gatccttcgt tttggcgtag tgcaactacg agtgtagcgg tcattataac
      301 tgctgaccag acctatatcc atcgtttgag tatcccggta gacgatcaat actatgtaag
      361 tgatcttcct tatttactgg ctattatcaa aaatgggcag ttcaactatt cttattattt
      421 attgggattg aaccgcgact ccatgaaatt gtatgaggtc agcaacaaac aagtaaaaga
      481 agtatctcta ccagaagggg caccggttga tgtcgttact gcattgggat ctgaactgac
      541 aggtggcggt ttgaacttct cttctaatgg aggaggcgga gcccgagatg gagtaacttt
      601 ccatggcgta aatccaaaag atgaagaagt agagatcgat tgggtcaact attaccaagc
      661 tgttgatacg tacttgaagg attctttcga aaatcctgaa caatggccga tttatttatt
      721 tgcattacca gaaaatcaga cgatgttcaa aaaaattgct aaaaatccct attattgtgc
      781 agacgttgcg atttctgctt ctcctgcgca gctatccttt agagatatcg aagaaggggc
      841 gaagaagctg tcaagtgaac tcgcagctca agaatcaaaa gaatacaata aactcttaga
      901 taagaaattc atcgatcagt tagttgatat cgttccagct gcgaaagaag gaaaagtgtc
      961 acatttattt atcgctacgt caaacttagc tgatggtttc ggtgaagatc ctgaaacaga
     1021 atatgatcgg cgtcaagtgc tgaatgcatt agcagatgat gtgatcatta atggcgggaa
     1081 agtgtttatt ttggatcaaa aagatgcgcc agatgaaaag agtctgacag cgattcttcg
     1141 ttattaaaag aggttgtgaa atcagtgttt tgacctgagt attagagaaa caaacggaaa
     1201 aatagtttca catatttttc cgttttttga gctaataccg aaggtcagct gattaaacac
     1261 cgtgtatgag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gcaagcaaaa
     1321 atagctcttc atattttttg tttggtgggc ttattaccgc agatgcaagc ttttgaacac
     1381 cgtttaagga agaggttgtg acagaagtgg tcagcttcaa gaaataagaa gagattgaca
     1441 aaaattattt ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc tacttctgga
     1501 acaccgttta
//
LOCUS       DASJTM010000226         1497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJTM010000226 DASJTM010000000
VERSION     DASJTM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.234"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1448
                     /locus_tag="U4B29_003051"
     CDS             <1..1448
                     /locus_tag="U4B29_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN2107930.1"
                     /translation="GFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGGLG
                     LPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFGV
                     STLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLIV
                     GTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRNL
                     IIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPRV
                     WAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFILT
                     PVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSFF
                     IVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTESA
                     YFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          403 a          286 c          316 g          492 t
ORIGIN      
        1 agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac
       61 gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca
      121 acgggggact tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt
      181 tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac
      241 gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat
      301 ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga
      361 aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg
      421 caggattttc gattttttac atgggtgtca acttaggggc attattagca ccactaatcg
      481 ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga
      541 tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta
      601 caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca
      661 tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa
      721 ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta
      781 tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata
      841 tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct
      901 tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa
      961 gctggtatca atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga
     1021 tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt
     1081 tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg
     1141 gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt
     1201 gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac
     1261 aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg
     1321 cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa
     1381 tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta
     1441 ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctgtttt
//
LOCUS       DASJTM010000227         1483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJTM010000227 DASJTM010000000
VERSION     DASJTM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.235"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..589)
                     /locus_tag="U4B29_003052"
                     /pseudo
     CDS             complement(1..589)
                     /locus_tag="U4B29_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1266..1339)
                     /locus_tag="U4B29_003053"
     tRNA            complement(1266..1339)
                     /locus_tag="U4B29_003053"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Ile,seq:cat)
BASE COUNT          433 a          273 c          271 g          506 t
ORIGIN      
        1 tcattacaag ctgttcgttg ccctttaaaa tgagcgatcg tataggtaga tgtcaatccg
       61 cggcgtttca tgattcgacc gatccggcgc cgactgaatt gaagcccaca ctttgctaaa
      121 cacttcttca attttcgggt accgtaagcc tttcggtttc gaataaactc ttcctgaacg
      181 atctcttcta agtcggactc attttcgatc ggtttcgctt gataatagta ggtttgacga
      241 gaaatattta gaattttgca catcgctgat atggaatatt tatgcttgtt ggcatcaatt
      301 acttgtcttt tcgtccgaat atcagcgccg cttgctttaa aatatcattc tccatttcga
      361 gttgcttatt tttctttctt agtgcgatca attctgcttg ttctggtgtt aagttgtctc
      421 tttctttgaa tgagcccgtt gattgtgctt gcttcatcca tttatcgaaa gaagaaggcg
      481 taagctcata ctctcgaata atttctgcgc gaggcttacc agcgagatag agatcgacga
      541 tttgttgttt gaattctttt gaataggttc ttcgttgacg tcttgacatg aaaaatcctc
      601 cagtgtgttt tttattattc tacacacctt attttttctg tctagtctag tgtagccgat
      661 tcatgactga cgtgccggac gcacgtagcg atcatcggcg ttatgactat ataaagtata
      721 ctttcgttga tccagaaagg tataaagatt aaagtaggct acctaaaaag gtagcccgga
      781 acgaatttta tcaccatact tgtgaaagga gatatttttt aagttagtat taagattgtg
      841 taatatgatg atatctatat tttatagtat cagtgctata aaatcaaaaa taagtcacta
      901 attaactacc actccaattg taagcctttt ttctcacttt ttttcaaaaa tatggtatgc
      961 tttttaatgg cttcaaatat aaaagagttt aaagcgtaac acacttatgg ggaagtggtt
     1021 tggggtgcgc tttaaactct tctttattat tatctcacaa tttaacccaa atgtctttct
     1081 atttaaaaat caaagtaaaa cttttcaaat atacagaagt ataactatgt gaaacatcct
     1141 ttcattaatc cataaaagga tacataaaaa agccactcat ttgagtggca atgaagaaaa
     1201 gctttagctt gtataatact cttcaaaaaa attctaacac agaacgattc aaatggctac
     1261 gttaatgtac cctgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctga
     1321 ccaactgagc taagggtact ggttgttgcc acataaagcc ataaacaatc aaccagtaga
     1381 atgtgtggca acaaacctgt tatcgcatag cttggagtgt gactatttat gggtgatagt
     1441 gaagatatgc gataacatca ctattttatc gaataatttt tat
//
LOCUS       DASJTM010000228         1482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJTM010000228 DASJTM010000000
VERSION     DASJTM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.236"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="U4B29_003054"
     CDS             <1..828
                     /locus_tag="U4B29_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN2107931.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="U4B29_003055"
     CDS             828..1448
                     /locus_tag="U4B29_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107932.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DASJTM010000229         1434 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJTM010000229 DASJTM010000000
VERSION     DASJTM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.237"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..>1434
                     /locus_tag="U4B29_003056"
     CDS             130..>1434
                     /locus_tag="U4B29_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2107933.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLIL"
BASE COUNT          461 a          174 c          277 g          522 t
ORIGIN      
        1 gaattattag gagaaaaaac aaataatact ctgaaatgta aaaaataaat actgactctt
       61 tacgcttcag agtatagtac gtactttcat cagaatgaaa acgcttaaca acagaaggga
      121 gagaattgta tggaaagaaa atttggtata agagacaaaa taggatatat gtttggagat
      181 tttggaaacg attttacttt catttttgct agttcttttt taatgatctt ttatacaaaa
      241 gtattgggga taagcggagc tgccgttgga acattatttt tagtagcaag atttatcgat
      301 gcagttactg atgtaactat ggggcgtatt gttgatagat cgaaagcaac taagcagggt
      361 aagtttaaac cgtggatttt aagaatgagt ggtccagtcg ctttagcaag ttttttaatg
      421 tatcaaactt ctatgatttc agcttctatg agcgttaagg tagtatacat gtatgttacg
      481 tatatattat gggggagtat tttttacaca tctataaata ttccttatgg atcaatggca
      541 tcagctatta cttctattac agaagaaaga acaatgctat cgacattccg aaatgttgga
      601 gctactttag caagtttggt cattggtgta ttaacaccat tatttattta tacaagagat
      661 gattcaggcg ctcaaatagt tcgtggtgga tcaactttta caattgttgc gggaatattt
      721 tcgatatgtg cacttatttg ttatttaata tgttataaac taactactga acgagtaaga
      781 attgaccaat ctcaatacaa agtttcattg ttaaaatctt taaaagggtt agttaaaaat
      841 agggcattac tttctataat tggtgcggct attttcttac tccttgctca attattaatt
      901 atgagtatga ataattatgt atttcctgat tactataatg atgcacaggg aatagtttta
      961 atgaatttta tcaatcctat tttggtttta atagttgttt cgcctttaac tttgttttta
     1021 agtaaaaaat atggaaaaaa agaactggca tcagtaggaa tgttcttttc agcaatagta
     1081 tatttgatct tatttttgat aaaagctagt aatatgtatg tattcttaat tcttagttcg
     1141 gttggctata tgggtttggg tgtgtttaat actgttattt gggcaaatat tactgatgtt
     1201 attgatgatc aagaagtaaa gtctgatcaa agagaagatg ggaccgtata tgcggtatat
     1261 tcttttgcta gaaaaatagg tcaagctcta gctggaggcg tcggaggttg gactctttct
     1321 attataggct atgactcttt agcaaatgta caaacgggag atgttttaga aaagttatat
     1381 aacgcttcaa cattaattcc atcaatttgt tttctgattg taggattaat ttta
//
LOCUS       DASJTM010000230         1430 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJTM010000230 DASJTM010000000
VERSION     DASJTM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.238"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..144)
                     /locus_tag="U4B29_003057"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="U4B29_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="U4B29_003058"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="U4B29_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2107934.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          414 a          257 c          236 g          523 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat
//
LOCUS       DASJTM010000231         1428 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJTM010000231 DASJTM010000000
VERSION     DASJTM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.239"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4B29_003059"
     CDS             131..505
                     /locus_tag="U4B29_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2107935.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4B29_003060"
     CDS             complement(619..1248)
                     /locus_tag="U4B29_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2107936.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          481 a          214 c          251 g          482 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaag
//
LOCUS       DASJTM010000232         1417 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJTM010000232 DASJTM010000000
VERSION     DASJTM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.240"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4B29_003061"
     CDS             complement(150..1337)
                     /locus_tag="U4B29_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN2107937.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DASJTM010000233         1407 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJTM010000233 DASJTM010000000
VERSION     DASJTM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.241"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1350
                     /locus_tag="U4B29_003062"
     CDS             <1..1350
                     /locus_tag="U4B29_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2107938.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          557 a          224 c          304 g          322 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttt
//
LOCUS       DASJTM010000234         1398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJTM010000234 DASJTM010000000
VERSION     DASJTM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.242"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..665
                     /locus_tag="U4B29_003063"
     CDS             <1..665
                     /locus_tag="U4B29_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2107939.1"
                     /translation="FIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLND
                     NGQSYADNFTIQGLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIG
                     NAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMK
                     DKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGE
                     IL"
     gene            662..1381
                     /locus_tag="U4B29_003064"
     CDS             662..1381
                     /locus_tag="U4B29_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2107940.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
BASE COUNT          492 a          156 c          263 g          487 t
ORIGIN      
        1 tatttataat atttgttgca atagtattca ctttaaaagt tgattttaaa gtagtgatca
       61 agatattatc atgcgtaata cttgtgtcga tagtattatc cttttttata acaatattta
      121 ttacattgaa cgataatgga caaagctatg ctgataattt cacaattcaa ggtcttttag
      181 aatatgcgtc aagagattct ggatatgatg gtgttggtga tttaaatcga ataactggta
      241 tctctagagt agtagatgaa atatttaata ataattttag attagtccta atgggagtag
      301 gcattggcaa tgcagagttt accaattttt ttgtcagtga gttttataaa tcgtttggta
      361 atcttcatta tcagtatttt catcatattt ggttatttat agaagttgga atattaggag
      421 tttcgatatt cctatttttt ataataaaga catttttcaa tgcaaaaaga tcttttatga
      481 aggataaaga tgcaagtctt gttaaagtta ttgcaattat gtcggtattc ttttttgtgt
      541 ataacacaac acttaggggt gaaacaacgg ggccactgat ttatatgatt ttagccatgc
      601 ctgaagttgt gaaaaggaaa gagcattatt atagtgaaaa aaatacaaaa ggagagatat
      661 tatgattcct aagaagatac attactgttg gttcggggga aatccacttc cgaaaaaaga
      721 tagaaagtgc atagaaagct ggaaaaagta ttgccctgat tatgagatta ttgaatggaa
      781 tgaatcaaat tatgatttaa ataagaaccg ttatatggcc gatgcatata aagaacgtaa
      841 atggggtttt gttccagatt atgcaagatt tgatattatt tataatcatg gtggatttta
      901 tttagataca gatgttgagc taattaagtc gttagatata cttcgtgaaa atcatgccta
      961 tatgggattt gaaggggaaa tctgggttaa tggcggtatt ggttttggtg cagaagaagg
     1021 acattctgtt attaaaggtt taagggatat ttacgattca ttaagttttt atcaaaatga
     1081 tggtacattg aacttgaaac cgagtccgta ttatattaca gattatcttg aatctattgg
     1141 attggttcgc gataacagcg aacaaataat aaatggcctg catatctatc ctacggatta
     1201 ttttgctgcc aaggattatg atactggaaa aatttataaa actagtaata caataagcat
     1261 tcatcaatat aatgcatctt ggaaaacacc taaacaaaaa tttgtttata gattaaaatc
     1321 aacaattgga caaaaaaaat ttaacaggtt acttgcactg aaaaaatttt tacacttata
     1381 aaggagaaaa gtactttg
//
LOCUS       DASJTM010000235         1395 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJTM010000235 DASJTM010000000
VERSION     DASJTM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.243"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..420
                     /gene="spx"
                     /locus_tag="U4B29_003065"
     CDS             10..420
                     /gene="spx"
                     /locus_tag="U4B29_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2107941.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="U4B29_003066"
     CDS             434..790
                     /locus_tag="U4B29_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN2107942.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          209 c          261 g          414 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
      961 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
     1021 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
     1081 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
     1141 ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga
     1201 tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact tgtctctctg
     1261 atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt
     1321 agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat
     1381 tcaagacaac aaaca
//
LOCUS       DASJTM010000236         1358 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJTM010000236 DASJTM010000000
VERSION     DASJTM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.244"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1358
                     /locus_tag="U4B29_003067"
     CDS             82..>1358
                     /locus_tag="U4B29_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN2107943.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          462 a          262 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatca
//
LOCUS       DASJTM010000237         1357 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJTM010000237 DASJTM010000000
VERSION     DASJTM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.245"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..391
                     /locus_tag="U4B29_003068"
     CDS             98..391
                     /locus_tag="U4B29_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107944.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            388..612
                     /locus_tag="U4B29_003069"
     CDS             388..612
                     /locus_tag="U4B29_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2107945.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            609..>1357
                     /locus_tag="U4B29_003070"
     CDS             609..>1357
                     /locus_tag="U4B29_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2107946.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT          464 a          221 c          286 g          386 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
      421 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
      481 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
      541 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
      601 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
      661 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
      721 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
      781 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
      841 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
      901 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
      961 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
     1021 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
     1081 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
     1141 acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta
     1201 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
     1261 cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa
     1321 tcgaaaacgc aaacttgcat gtggctggat ggcacat
//
LOCUS       DASJTM010000238         1346 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJTM010000238 DASJTM010000000
VERSION     DASJTM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.246"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="U4B29_003071"
     CDS             <1..1244
                     /locus_tag="U4B29_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN2107947.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          438 a          244 c          286 g          378 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgcttac
     1321 ttttcttctt caagaccaaa taaaaa
//
LOCUS       DASJTM010000239         1337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJTM010000239 DASJTM010000000
VERSION     DASJTM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.247"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..237)
                     /locus_tag="U4B29_003072"
     CDS             complement(<1..237)
                     /locus_tag="U4B29_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107948.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWR"
     gene            complement(568..801)
                     /locus_tag="U4B29_003073"
     CDS             complement(568..801)
                     /locus_tag="U4B29_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN2107949.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(816..>1337)
                     /locus_tag="U4B29_003074"
     CDS             complement(816..>1337)
                     /locus_tag="U4B29_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2107950.1"
                     /translation="DPHAWLNLENGIIYAKNIEKQLAEKDPDNKKFYKENLDKYIEKL
                     DSLDKEAKSKFASIPNDKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKH
                     LVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDSIAEKGQDGDSYYAMM
                     KWNLDKIAEGLSK"
BASE COUNT          410 a          240 c          169 g          518 t
ORIGIN      
        1 tctccaactt ttctctatat ctatattttc taagtttaag aggtatttgg taaactcttc
       61 attatacata ttaataagcg cattaatatg cggctgaata atttcttcaa ataacgcttc
      121 cttagaagta aaatgtttat aaaaagcacc cgtagttacg cctgcggtca ggcacagttg
      181 ccttatctta gtttgctgaa atccttcttt ttcaaaatgg cttagtccac tttctattaa
      241 cttttggtga gttataatat aatctctggt ttctatgggt acttcctcct aataagatga
      301 attaacttta ttatcttaca caggacttga agtttcaatc gttaacgttt attactgaac
      361 tcattcctta gagctaaact aatgagaaag agcagaataa ctgatacaaa aagcaatcct
      421 acaagaatta atagactcat ctaaatcatt caccttttct aaaataatcc gagaaagatt
      481 taaaaaaatt atatccagct attaggataa gtcctatcat aatcactcca atagccattt
      541 tcttatcctc cctaaaaata aagtctttta cttaaaacct tttttgtaat cgtcaataaa
      601 ttttttccct ttatcagtta attgaaatcc tatatctaaa tagtcctcat atctaagttt
      661 agccagcatc tcacttacag atgctggact aacacttaga acttttgcta aatcattatt
      721 ttttaatttc ttttttcttt ttgataattc ctgaattgct agtaaataaa ttagtatagt
      781 agtcgtttta atttgtacca tacctaccct ccaaattatt tcgaaaggcc ttcagcaatt
      841 ttatccaggt tccatttcat catcgcatag taactatcac catcttgtcc tttttctgca
      901 attgaatcag taaaaatcgt tgaatagata ggaatattgg tatcttttga tactgttttc
      961 attggtctat catccacact gctttctacg aataaggagg gaacttttgt tgtgcgtaat
     1021 ttttcaacta agtgttttat ttgatctggt gttccttctt cttcagtgtt gatttcccaa
     1081 atgtaagcag aaggcacatt atacgctttc gagaaatatt taaagcatcc ttcacttgta
     1141 acaatcattt ttttatcatt cggaattgaa gcaaatttag atttagcttc tttgtctaga
     1201 gaatccaatt tttcaatata cttatctaga ttttctttat agaatttttt attatcagga
     1261 tctttttccg ctaactgttt ttcaatattt ttagcgtaaa taataccgtt ttctaaattt
     1321 aaccaagcat gaggatc
//
LOCUS       DASJTM010000240         1313 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJTM010000240 DASJTM010000000
VERSION     DASJTM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.248"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..124
                     /locus_tag="U4B29_003075"
     CDS             <1..124
                     /locus_tag="U4B29_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2107951.1"
                     /translation="DYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            129..1076
                     /locus_tag="U4B29_003076"
     CDS             129..1076
                     /locus_tag="U4B29_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN2107952.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          426 a          187 c          277 g          423 t
ORIGIN      
        1 agattatcta attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt
       61 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
      121 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
      181 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
      241 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
      301 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
      361 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
      421 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
      481 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
      541 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
      601 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
      661 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
      721 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
      781 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
      841 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
      901 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
      961 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
     1021 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
     1081 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
     1141 gtaaaatcaa atctgggata gaagggctct ttgtcaaatg atgttggtaa agagaaatct
     1201 tagaataatt tggacagaac agaaagaaga aaacctcttt ctgttctgtt tatttggtta
     1261 aaaatgtttt gccatgacga atcataattc gtagtttgaa attgtaaaag ttg
//
LOCUS       DASJTM010000241         1287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJTM010000241 DASJTM010000000
VERSION     DASJTM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.249"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..937)
                     /locus_tag="U4B29_003077"
     CDS             complement(<1..937)
                     /locus_tag="U4B29_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2107953.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLD
                     SKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQ
                     LFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIF"
     gene            complement(971..1287)
                     /locus_tag="U4B29_003078"
                     /pseudo
     CDS             complement(971..1287)
                     /locus_tag="U4B29_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
BASE COUNT          435 a          233 c          147 g          472 t
ORIGIN      
        1 caaaaattgt atagattaac tcatactctc ctttgggtat ttcaatacag taaatatctt
       61 tgatttctga aaaaaaatct tttgatacat ttaaaaaagt atcaaatagt tgcgctccct
      121 ctttcaaatg ttcatttgtt attccatcac caatcataat tcgttcaatc attccagaaa
      181 gatgtagaaa tagattaatt ctcagaaaac ttgggatttt gacatcgtaa tatttttcat
      241 acaaatatag tactttttct atctcattga atactttttt ggaatctaaa aagcgaagct
      301 tagtactagc gccttcaata gtaaagagtt taactaattc atttaagcag aactcacagc
      361 tatccgagtc catatactta tttaattttt ctaaggtttg attcccatta attagatttt
      421 ctaaatttaa acaatcgatt ccttgaatgt aaaattcagt ggttgaaaga acacaaattg
      481 tattcttaaa aatcatagta tctttttcct ttgctatttt ctttaatgcg ttaaaatcaa
      541 aaatcaaaat ctccacttga ttatcgagat accgttttaa aatatcctta actttttcag
      601 aaatcccctc accagagaga caagatacta ttacattttt cttttgtgat aatcctttat
      661 aatactgaac tttaacttga aacgaatcaa aatccatctg gtaaaaatga ctaattggct
      721 ttttttggca aacttgaatc ccacattcaa tcgctaaagc tgtagaaaca ttgtttaaaa
      781 ttaccaaatc acctattaca ttggattcaa ttttttcata catttgttcc aatgatccca
      841 tatcaactag cagaatgagt ccattcgtcg tatctatact tttacaaaaa ttgttgatta
      901 ggttaattaa gtcactagtc tcaccttcaa ttggcatatc gaaagcctca taaacatatg
      961 tattcagcaa cttatttgtt tcccttgcga tcgcagaagc tgttatttta ccgtgcataa
     1021 ctatcagaca atgggtttct ggtattttca catagaactt ctttacaaga aaaagtatcc
     1081 atttcaacaa aagttcttgt ttactttctt cgacttctaa actactccaa atctcatttg
     1141 ccaataattt ttgtttatca tttttcatac gaaattttct gaacttagac agcaaaaaat
     1201 ttcttgatgt ttcttcaatt acatcaataa acattataaa tagactgata tccgccaaga
     1261 attcatctaa taaacataga cctgtcg
//
LOCUS       DASJTM010000242         1275 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJTM010000242 DASJTM010000000
VERSION     DASJTM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.250"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="U4B29_003079"
     CDS             complement(31..1203)
                     /locus_tag="U4B29_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN2107954.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DASJTM010000243         1272 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJTM010000243 DASJTM010000000
VERSION     DASJTM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.252"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..466)
                     /locus_tag="U4B29_003080"
     CDS             complement(<1..466)
                     /locus_tag="U4B29_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN2107955.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRI"
     gene            complement(463..1080)
                     /locus_tag="U4B29_003081"
     CDS             complement(463..1080)
                     /locus_tag="U4B29_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2107956.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          445 a          230 c          138 g          459 t
ORIGIN      
        1 aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc ggtatccata aataatgtat
       61 cccacgatac tgtacagcca tctttgaatt gaatttcctt aaagcaaatt atactcattt
      121 ttcctgtcga attaaacttt gacccaatat ctaaaactgc atttttcctt atgtcaatat
      181 gagccccttg tgcgataaac gctggccctt gtattcttag tattccttca atacttataa
      241 tcgtttcttc atttcccgca gtatttaata ctggaaagcc caaataaact atttttccag
      301 attgtaatac tatctcaatt ttccctctcg atttgcgtgt aatccgtact ttacgatgca
      361 ccaaaatagg taatctccac gcatctgaaa tatgaagaaa tttataattg aagtatatcg
      421 ttttaggtaa atttaatata ttctttatta tcaatccgag tctcatcttt caccatctct
      481 ttttgtaatc aaaattagtt tataaaggat tacacccaat ataccgccca acgtattata
      541 tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa agttgtaaaa attcaattat
      601 tgctgaaaaa taaaatgcaa tttttcctcc atatactatt gaggaccata ttttgggttt
      661 ctttatattt tcatatttaa tagacattac catataaaca tatggaataa aaagaataat
      721 attttctaaa cacgctgtag ataactcttt tgttccagtt gttgtattat acttataaat
      781 ccaccagtct cccataacat cagataaagg atttaaccaa attgttcgat tgattaatgt
      841 tgtaaataaa atcatcgcag tatagaatat caaagcacaa acttttctat attttctatc
      901 cttctttaaa tgaagataaa gttccattag tatacgttta tagccctcac ctgcagcaat
      961 gccatctgtt gcatacataa aatagaccag tcctaaaatt gtcaataaaa cagcaaaccc
     1021 aaagggttca tataatttat ataatatatc tgtaataatt tctgcaatca ctattatcat
     1081 aattatttct ccaatatcaa gtatacactt atcgcttcca attacacatg atcatattag
     1141 agtgtattat cacattctct aatattcaat ttatttttcc taaaatattc gaaataatcg
     1201 tcttgataac gagtaagtat ttttatacga ttgataacat agtaacaata tttttaatta
     1261 tagtaaaaaa tc
//
LOCUS       DASJTM010000244         1252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJTM010000244 DASJTM010000000
VERSION     DASJTM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.253"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(407..1111)
                     /locus_tag="U4B29_003082"
     CDS             complement(407..1111)
                     /locus_tag="U4B29_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107957.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          368 a          216 c          228 g          440 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaaccg cttttttctc tatcagaaaa tatgtttatt attagaattt
      181 tttatagtta aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga
      241 agagaagtcg cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat
      301 ttgagcctga tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa
      361 aaaggctggg attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa
      421 tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc
      481 gttcgagtgc tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt
      541 tacgaggttc atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt
      601 aaaatttagg atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg
      661 tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg
      721 gcacactgac tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt
      781 ttttcaatcg ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc
      841 gttcgaaaat cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat
      901 cgattggcac gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga
      961 tttgacggtt tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct
     1021 cttcttctag agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat
     1081 ttaataattt ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg
     1141 attataagaa aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca
     1201 aattacaaca gaaaaatttg cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DASJTM010000245         1246 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJTM010000245 DASJTM010000000
VERSION     DASJTM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.254"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4B29_003083"
     CDS             200..742
                     /locus_tag="U4B29_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107958.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="U4B29_003084"
     CDS             898..>1246
                     /locus_tag="U4B29_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN2107959.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DASJTM010000246         1235 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJTM010000246 DASJTM010000000
VERSION     DASJTM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.255"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            70..1026
                     /locus_tag="U4B29_003085"
     CDS             70..1026
                     /locus_tag="U4B29_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107960.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1235)
                     /locus_tag="U4B29_003086"
     CDS             complement(1080..>1235)
                     /locus_tag="U4B29_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2107961.1"
                     /translation="GLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYV
                     NGKFCLA"
BASE COUNT          429 a          184 c          210 g          412 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaattccat ttcttttggt agtcc
//
LOCUS       DASJTM010000247         1201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJTM010000247 DASJTM010000000
VERSION     DASJTM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.256"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(72..983)
                     /locus_tag="U4B29_003087"
     CDS             complement(72..983)
                     /locus_tag="U4B29_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN2107962.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          349 a          250 c          172 g          430 t
ORIGIN      
        1 gctaacgtta ccccaaaagt gctagtacaa taatgctgtt cattaatttc gttggtttca
       61 ttccgccatc cttacctcct tgaattagta tgtgttgatc cgggaaacac tgtcacaatt
      121 acaccgtcta tattgtttcc tgatatctca taacgaagat tagtcggcac atgaatggtg
      181 gtatttttag taacaggata ataaaagatc tcatattttt ccccatttac actaacagaa
      241 atggtttttt cttcttttag gtaatccata tattcctcga gaacgaaatt cttttctttc
      301 ataatcgcac tatgtggtaa accaacatag cgaatatgcc atggttcata ttgaattcct
      361 gttaactctg ttttgtcctc tggataacgt aaaatgaacc cgtatttcca agcattttct
      421 tctatccact ttccttcagg ggctcgttcc attttcgtca agcttgatcc tacatctagt
      481 gataaacctg aattatgctc actataacct gctggtaagg catactcagc ccccatttct
      541 tggtaaagca cactttgctc atcaaagtct cgatagccac tattaataat aaaatgacta
      601 acgccaccct ttacagcatc attgaccatc tctgaaaatt tttgtgctat ttcttttgac
      661 atataaatat tactatcaag caacccgtat ccatttatta attcgtcatg tttagataaa
      721 ttcacgatat ctgacttcac actttcttgg cgaacaggat atttactatt gattaatagc
      781 agatttcctt gataaacctg ttcttctgta attgttttct cttgggtatt ttcagaagtc
      841 ccactatttt ctaaatgttc tttgggattt tgatcataat tttgaaattc gactttttcc
      901 tgagaaaaca gtgcttcatt aacgtagtca taacctaagt atattaagaa taacaataac
      961 aataaaaaaa acaacttctt cattttcagt ctcctcctta actatatagt tggaagtatg
     1021 aaatatttct agaatcttcc ccttaaacgg cacaatctcg tttgagattg tgccgttttg
     1081 ccccgtaaat tacagtcaat tatttcgccc tactggattt actattatca ccaatgtaga
     1141 tgaattttca taagtgatac ccatgctgaa tttaacgagg ctgaactgcc tgttgacaat
     1201 g
//
LOCUS       DASJTM010000248         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJTM010000248 DASJTM010000000
VERSION     DASJTM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.257"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(95..394)
                     /locus_tag="U4B29_003088"
     CDS             complement(95..394)
                     /locus_tag="U4B29_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2107963.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            745..>1148
                     /locus_tag="U4B29_003089"
     CDS             745..>1148
                     /locus_tag="U4B29_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2107964.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHS"
BASE COUNT          384 a          189 c          161 g          414 t
ORIGIN      
        1 ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta
       61 tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg
      121 ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat
      181 aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt
      241 ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta
      301 tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta
      361 gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt
      421 cttcccacta cattctataa acaaagtata cgctacatct agattttttt aaatcattac
      481 tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc
      541 taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt
      601 gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa
      661 ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact
      721 ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat
      781 aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca
      841 caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc
      901 aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc
      961 gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt
     1021 gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc
     1081 gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt
     1141 gagcactc
//
LOCUS       DASJTM010000249         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJTM010000249 DASJTM010000000
VERSION     DASJTM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.259"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..>1148
                     /locus_tag="U4B29_003090"
     CDS             37..>1148
                     /locus_tag="U4B29_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2107965.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKA"
BASE COUNT          365 a          232 c          221 g          330 t
ORIGIN      
        1 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
       61 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      121 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      181 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      241 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      301 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      361 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      421 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      481 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      541 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      601 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      661 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      721 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      781 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      841 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      901 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
      961 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1021 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1081 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1141 aaaaaagc
//
LOCUS       DASJTM010000250         1145 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJTM010000250 DASJTM010000000
VERSION     DASJTM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.260"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..278)
                     /locus_tag="U4B29_003091"
                     /pseudo
     CDS             complement(111..278)
                     /locus_tag="U4B29_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="U4B29_003092"
     CDS             complement(544..738)
                     /locus_tag="U4B29_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107966.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="U4B29_003093"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="U4B29_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          403 a          190 c          163 g          389 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaat
//
LOCUS       DASJTM010000251         1133 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJTM010000251 DASJTM010000000
VERSION     DASJTM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.261"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..250
                     /locus_tag="U4B29_003094"
     CDS             44..250
                     /locus_tag="U4B29_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107967.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(612..851)
                     /locus_tag="U4B29_003095"
     CDS             complement(612..851)
                     /locus_tag="U4B29_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107968.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          395 a          162 c          210 g          366 t
ORIGIN      
        1 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
       61 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      121 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      181 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      241 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      301 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      361 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      421 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      481 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      541 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      601 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      661 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      721 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      781 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      841 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      901 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      961 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
     1021 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
     1081 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tag
//
LOCUS       DASJTM010000252         1067 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJTM010000252 DASJTM010000000
VERSION     DASJTM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.262"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="U4B29_003096"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="U4B29_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2107969.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DASJTM010000253         1049 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJTM010000253 DASJTM010000000
VERSION     DASJTM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.263"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            166..591
                     /locus_tag="U4B29_003097"
     CDS             166..591
                     /locus_tag="U4B29_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107970.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT          368 a          172 c          178 g          331 t
ORIGIN      
        1 ttttttaaaa cgctttctga aagtggcgga ttttttgcct ttttaaaatt ttattctaaa
       61 atttactcta tttgtgggtg ttttgtgcgt ttttctcttc catccgtttg aagttttaca
      121 ttccaatggt ataatttctt tatcctatca aatgggggaa gtattttgaa gaaattaatc
      181 gtactattta ttttgtctgc gggacttttg acattgactg gctgtgattt gtttggcggc
      241 tctgaccaat cctcggacga aacaaccgat tcttctgtct taaaagcgga aaaagcagat
      301 accaccaaca gtgtatatac gctaaaagta cgtaaagata tggatttatc cgcgggacag
      361 ttgacattta cagataaaaa aatggaatgg acacgcacgt atcagacaca aggaactgat
      421 tcttctgaat catctgaaga aaaaacagtg ctgacagata tcaagatcac taccaaagat
      481 gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa caacagtcat atttacaaaa
      541 atcggtaatt accgtttgaa ggatgaggat ggcaacatct acagtctata gtgccgtctg
      601 ttggatcttc ttttgtttct ccccttcttt tctgctatag taaagaagca agatgattgt
      661 aaaggagacg aaatggatga tcgatagtcc tgattattgt acatgtaata acaacaaaaa
      721 tatccataca gaatttaatg accaaggaag cttgagcata tgcgacaact gtcacaaact
      781 agtcaaagat tcatttaaaa catttgaatc ttatcctgtt caaaattatg taaaaagtag
      841 aagaagataa acaaatccaa gcaaaactca ctatggctga tttttatgaa cttcaaactc
      901 agcttcttcc tttgatcaag cttcatcagt aaaacaaaca gtggatcatt tcagtttaca
      961 aatcaaagag taaagattta tttatttcaa atatttgaat ataaaataac caaacgatag
     1021 aaaggcttat tctacatcac gtagtaatt
//
LOCUS       DASJTM010000254         1030 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJTM010000254 DASJTM010000000
VERSION     DASJTM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.264"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..988
                     /locus_tag="U4B29_003098"
     CDS             179..988
                     /locus_tag="U4B29_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2107971.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          341 a          179 c          198 g          312 t
ORIGIN      
        1 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
       61 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
      121 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
      181 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
      241 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
      301 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
      361 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
      421 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
      481 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
      541 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
      601 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
      661 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
      721 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
      781 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
      841 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
      901 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
      961 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
     1021 gacaattttt
//
LOCUS       DASJTM010000255          993 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJTM010000255 DASJTM010000000
VERSION     DASJTM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.265"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..886
                     /locus_tag="U4B29_003099"
     CDS             <1..886
                     /locus_tag="U4B29_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN2107972.1"
                     /translation="VPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKI
                     IKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEE
                     KVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELE
                     VIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEF
                     DVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTF
                     TKLAKAEQYRRSLELSKD"
BASE COUNT          438 a          104 c          165 g          286 t
ORIGIN      
        1 tgtccctctt attttaaata aacgagagaa aaataattta aagaaaaaaa tgctcattcc
       61 atttgattca gaagtgaaag gattactcaa taaactaact atttctaata tagactcgac
      121 tagggaaaaa atcataaaaa tatttgataa aattaataat ttattcaaga atgacaatgc
      181 tgaattaagg aagggtcttt tagagtttaa acttcaagaa ctagaacttc attactctta
      241 tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac agatgataga
      301 agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata aagaacaacg
      361 tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta atgcagatgt
      421 ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa aagaattaga
      481 agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt atgtgtatgt
      541 aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga ctagaagatt
      601 agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg aatttgacgt
      661 tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc ataatcattt
      721 tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag tttcactaga
      781 cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta caaaattagc
      841 taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat ataaaaagcc
      901 ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat agaaataatg
      961 tggttagaaa aaataaatga caaaaaattt aag
//
LOCUS       DASJTM010000256          991 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJTM010000256 DASJTM010000000
VERSION     DASJTM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.266"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            359..991
                     /locus_tag="U4B29_003100"
                     /pseudo
     CDS             359..991
                     /locus_tag="U4B29_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          181 c          182 g          289 t
ORIGIN      
        1 agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat
       61 tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta
      121 ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt
      181 aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt
      241 catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat
      301 aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat
      361 gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc
      421 aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt
      481 tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc
      541 tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac
      601 agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt
      661 cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca
      721 agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa
      781 ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg
      841 cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct
      901 ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt
      961 tagagacaag gaatcagact tattttttac c
//
LOCUS       DASJTM010000257          982 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJTM010000257 DASJTM010000000
VERSION     DASJTM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.268"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..982
                     /locus_tag="U4B29_003101"
                     /pseudo
     CDS             1..982
                     /locus_tag="U4B29_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          349 a          174 c          196 g          263 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg ca
//
LOCUS       DASJTM010000258          982 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJTM010000258 DASJTM010000000
VERSION     DASJTM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.269"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..947
                     /locus_tag="U4B29_003102"
     CDS             <1..947
                     /locus_tag="U4B29_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2107973.1"
                     /translation="FGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIV
                     IDIDTLLSLYTTRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVL
                     TEEDRQKHIDEYSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMIS
                     AWKIVEKKVDDWDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYV
                     LSSVYEGFPMVILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIM
                     FTKNTDLAMRMSLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          370 a          100 c          199 g          313 t
ORIGIN      
        1 attttgggtt agataagacg attaaggttg attatatgtt aaatcctaaa acggagggaa
       61 aattatatag aacttattta tatccggttt ggaaattgca caagtatata tgcaaaaatg
      121 aatttgatat agtaattgat attgatactt tgttgtcttt gtatactaca agggcaatta
      181 aaggaacaaa gtgcaaattg atttcttggg aacattttaa ttactggaca atggaaatgc
      241 tgaaagaaaa gaagagattc aaggctaaaa aattgataaa aaaatatgca aagaggcttg
      301 tggttctcac agaagaagat cgtcaaaaac atattgatga atatagttta tcagaagatt
      361 ttgttataac tatgccaaat ccgtgtatat ctaatatcga gtacgaatat cattatgata
      421 aaaagagatt tcttgcagta gggcgattag tggaagagaa aaattttgct gacatgataa
      481 gtgcttggaa aattgtagaa aaaaaagtag atgattggga tcttatcatt gtaggaaaag
      541 gtgaattaga aaaagatctt cgccagcaga taaaagattt aaatctgcaa agagtaacta
      601 tagcagggca ttcagaccat gtggaggaat attataaatc tgcatcttgt tatgtgctta
      661 gttctgttta tgaaggattt cctatggtta tattagaagc acagtcttat ggattaccag
      721 ttatttctta tgattgtaaa actggtccga gagatttggt acatcataat tttaatggga
      781 tgttggttga agataagaat atagaccaat tagctaagag catgataatg tttactaaga
      841 atactgattt agcaatgaga atgtcactga acgcatataa taatgtacaa aaatttaatt
      901 tgaaagaaat tacaaaacaa tgggttgctt tgattgaaaa tgtatagttt tttgtggttg
      961 tccaaacatg aaaattagcg ta
//
LOCUS       DASJTM010000259          973 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJTM010000259 DASJTM010000000
VERSION     DASJTM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.270"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..97)
                     /locus_tag="U4B29_003103"
     tRNA            complement(24..97)
                     /locus_tag="U4B29_003103"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Ile,seq:cat)
     gene            complement(652..966)
                     /locus_tag="U4B29_003104"
     CDS             complement(652..966)
                     /locus_tag="U4B29_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107974.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          359 a          151 c          153 g          310 t
ORIGIN      
        1 aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga ccggacggtt
       61 atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac ataaagccat
      121 aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct tggagtgtga
      181 ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga gacagataaa
      241 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
      301 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
      361 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
      421 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
      481 catgttcctt caaataagtt ttttaattat aaactctaga gaaattaatt catcctaaca
      541 taaaataatt gttcactcaa aagtttttct agaacaatta aaacagcagg cttcacattc
      601 gctatacatt tgtaagaagt aataattcct gtactaaaag gcataattgc tttaattatt
      661 ttttgacttc tcttcaaaca caagaagtag ttgtgtaata attgcagaaa taaggattcc
      721 aataatagat ataaaatttg acatggaaac gtctataaca ttaaaagaat aaagtgttga
      781 agaaagtaaa ctgattgaaa taacccctcc gtaaaaaata gtctgtgaat atacccactt
      841 tttttcagaa gacgtacttc gtttggttct aaaacctatc cctgcatttc tactcttatc
      901 aaaaaaccaa atatttagaa gtaataatat tgctattatt aagataaaat tactgtaaaa
      961 atccaaaagc tat
//
LOCUS       DASJTM010000260          956 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJTM010000260 DASJTM010000000
VERSION     DASJTM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.271"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..189
                     /locus_tag="U4B29_003105"
     CDS             <1..189
                     /locus_tag="U4B29_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2107975.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT          299 a          182 c          121 g          354 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcga
//
LOCUS       DASJTM010000261          947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJTM010000261 DASJTM010000000
VERSION     DASJTM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.272"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..535)
                     /locus_tag="U4B29_003106"
     CDS             complement(<1..535)
                     /locus_tag="U4B29_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2107976.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEE"
     gene            complement(665..>947)
                     /locus_tag="U4B29_003107"
     CDS             complement(665..>947)
                     /locus_tag="U4B29_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2107977.1"
                     /translation="ILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVF
                     NEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          361 a          155 c          127 g          304 t
ORIGIN      
        1 tttcttcagc aatcatagtt gcacgtataa gaacgggaac taacagaaat tccaaatatt
       61 gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt taaagattct ttaatcattc
      121 ttatttcaga acgataagtc ggaaagaatc tgatcactac tgtaatactt aacattattt
      181 ttttcggtaa atgtattttt tctaaagagg ataataattc attgctagta tagacactaa
      241 ccaacaacca tgagatcatt acgaccggca acattcgaaa tacaatatat aaggatgtcg
      301 caaaaaatat agtaaaaaac tctatcttga tagcatcaat aattttcatt actaaaaaca
      361 gaactgaata aatcaagatg aaataaatac atgtctttat tttacgaaac atcaatacta
      421 aaactgtagc aataatgaaa caccccactt gaaatatatt tccctttcct aaaaaaataa
      481 tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt gacactcttt
      541 cacctccaaa aaataagatc gcactgttat cttaagctga taaaaatgca ccgttattca
      601 gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg caagcttttt
      661 ttaattaact cagtaattca atccacccag gaacagaaaa ttgatacaat cttctggtta
      721 atagcatagt cttttctttt gaatagctat gcttcaatat atcatagaat gtagcgtaga
      781 atgagtaaat gtagaagtgg atttcttctt cattaaagac taaattttga ttgatcattc
      841 cgatagtctt taacttatct agacaacaga ctgtcttttt cgtcacaaat gatgtaattt
      901 tttcaacaat atcttgatat ttggttccag ctgattggaa taaaatc
//
LOCUS       DASJTM010000262          925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJTM010000262 DASJTM010000000
VERSION     DASJTM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.273"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..>925
                     /locus_tag="U4B29_003108"
     CDS             5..>925
                     /locus_tag="U4B29_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2107978.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTK"
BASE COUNT          348 a          115 c          159 g          303 t
ORIGIN      
        1 agaattgttt gaatttaaca atgtttcttt ttcatataag gatgttaagg aacaagcgct
       61 tgttgacctt aatttttcaa taaaaaaagg cagtcttaca ttgattactg gttcttctgg
      121 gagtgggaaa tccacactat taaagttaat gaatggactt attccaagac tttatgacgg
      181 aaaattggtt ggggaaattt tatttgaaaa taactcgcta caatccttaa cacaagaaag
      241 tatatcaaaa aatattggct acgtaagtca agatcctaga ggacaattct ttacaacaaa
      301 tacaacgagt gagttagtat tttcgatgga aaactttgga tttacaaaag agcatatgga
      361 taaaagaatc aatgaattag ctgaattatt agatctttca aatatattag ataaaaacat
      421 tttttcgcta tcaagtggtg aacggcagaa aatttctatt ggatgtagtt tatcgctaaa
      481 ctctaaatta attatattgg atgaaccgtc ttcaaatttg gacttcaaag cgactaagaa
      541 acttgctgac ttactttgtg atttaaaaaa taaaggattt actattgtta tagctgaaca
      601 tcgtttttac tatttgaaag aattaattga tcaagttcta tttgttaaaa atagaactgt
      661 aaaaactaca actattcagt tgttaaaaac tgaaaacaat aacgcattaa ggaaatttga
      721 catttttaat agttcgattg aagttcccta tatcgaaaag gtaagtgcag atgtattaaa
      781 agttgaagaa attacttatg aaaatattct aaaagacata aattttgatg tcaaagttgg
      841 agatgttatt ggtttggtag ggaaaaacgg tgtggggaaa acaaccttac taagaatact
      901 catgaagatt attcgcccta ccaaa
//
LOCUS       DASJTM010000263          911 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJTM010000263 DASJTM010000000
VERSION     DASJTM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.274"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..441
                     /gene="vanZ"
                     /locus_tag="U4B29_003109"
     CDS             <1..441
                     /gene="vanZ"
                     /locus_tag="U4B29_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN2107979.1"
                     /translation="TLIWLVLFKLQYNILSVFNYHQRSLNLTPFTATGNFREMIDNVI
                     IFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGATDITDVITNTVGGFL
                     GLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINYV"
BASE COUNT          308 a          137 c          154 g          312 t
ORIGIN      
        1 acactaatct ggttagtgtt attcaaatta caatacaata ttttatcagt atttaattat
       61 catcaaagaa gtcttaactt gactccattt actgctactg ggaatttcag agagatgata
      121 gataatgtta taatctttat tccatttggc ttgcttttga atgtcaattt taaagaaatc
      181 ggatttttac ctaagtttgc ttttgtactg gttttaagtc ttacttttga aataattcaa
      241 tttatcttcg ctattggagc gacagacata acagatgtaa ttacaaatac tgttggaggc
      301 tttcttggac tgaaattata tggtttaagc aataagcata tgaatcaaaa aaaattagac
      361 agagttatta tttttgtagg tatacttttg ctcgtattat tgctcgttta ccgtacccat
      421 ttaagaataa attacgtgta agatgtctaa atcaagcaat ctgatctttc atacacataa
      481 agatattgaa tgaattggat tagatggaaa acgggatgtg gggaaactcg cccgtaggtg
      541 tgaagtgagg ggaaaaccgg tgataaagta aaaagcttac ctaacactat agtaacaaag
      601 aaagcccaat tatcaatttt agtgctgagg aattggtctc tttaataaat ttccttaacg
      661 ttgtaaatcc gcattttcct gacggtaccc ccactagcag ccatgctttt tttgaatctt
      721 tgtaggacgt tttaaagaga attacccact ttaaaattaa acatgcgctt agaatcgctt
      781 taggaaacac gatccagtcc aataatcgtc gataaaaact tttgaaaaag gttggtgaaa
      841 ttacctactt ttggaataat cacaaatcac aagtgattaa tcacttgttt attaagatat
      901 taaaagctat a
//
LOCUS       DASJTM010000264          896 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJTM010000264 DASJTM010000000
VERSION     DASJTM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.275"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            253..876
                     /locus_tag="U4B29_003110"
     CDS             253..876
                     /locus_tag="U4B29_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2107980.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          331 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaagaa
//
LOCUS       DASJTM010000265          894 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJTM010000265 DASJTM010000000
VERSION     DASJTM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.276"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..633)
                     /locus_tag="U4B29_003111"
     CDS             complement(<1..633)
                     /locus_tag="U4B29_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2107981.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSK"
BASE COUNT          295 a          166 c           96 g          337 t
ORIGIN      
        1 ttttgaatgt tgcaaatatg aaatatggaa tatgatatct tcagatatat aatccctttc
       61 tgacggaaac ttgatattgt gctgttgaat tatctcagat gaatacagcg catgccaaac
      121 tgacattggc aatataaaat cgactttata actcggctca gtgccaatca ttccaagcaa
      181 cactttttca attatagcgt tatttgtaaa tatcgtacta tcatatcttg ctggctttga
      241 aataatatta ttatcatcat aatattcata atatccagca taacatgtgt ctgcattact
      301 cctttttata ccatttaaca ttgcttcaat ataattagct gcaacaaaat catcagaatc
      361 aataaaagca acatattttc cttttacatg ttcaagtcct gtatttcttg ccaatcccaa
      421 gccagcatta tttttatgaa taatacgaat ccttgaatcc tttaatgcat agtcttcaca
      481 tattttatta gatgaatcct tagatccatc atccacaatt ataatatcta ggttcttata
      541 tgtttgatta cacactgatt ccaaacaact tcctacatac ttttcaacat tatatacagg
      601 tatgattaca ctaattagat cactcgaatt catccttcta tcctttcact cacgttttct
      661 caattagtca atgacggcat caacgatttt cttaaattct ctgaaaaaaa tagactctga
      721 tcttgatgat gtgaatgaca tactatcttc ttctagaaaa attatttcct ttaaattatt
      781 tattaaatca ataatatgat cctcgtaagt atgctttccc ttggaataat gaaaatatac
      841 ttttgtatta cacaatattg caattttatt tcttaatatg attatttcac tcat
//
LOCUS       DASJTM010000266          892 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJTM010000266 DASJTM010000000
VERSION     DASJTM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.277"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U4B29_003112"
     CDS             7..624
                     /locus_tag="U4B29_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107982.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          337 a          110 c          155 g          290 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt ag
//
LOCUS       DASJTM010000267          888 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJTM010000267 DASJTM010000000
VERSION     DASJTM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.278"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..254)
                     /locus_tag="U4B29_003113"
     CDS             complement(<1..254)
                     /locus_tag="U4B29_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2107983.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
     gene            complement(254..517)
                     /locus_tag="U4B29_003114"
     CDS             complement(254..517)
                     /locus_tag="U4B29_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2107984.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            751..>888
                     /locus_tag="U4B29_003115"
     CDS             751..>888
                     /locus_tag="U4B29_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2107985.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          285 a          176 c          119 g          308 t
ORIGIN      
        1 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
       61 tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa cttctcaaat
      121 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
      181 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
      241 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
      301 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
      361 ctttgattgc tttccaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
      421 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
      481 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
      541 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
      601 accaaaacag cattgtaaag tgttatccgt ttcctttata cccgtggtgc tagttaattt
      661 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt
      721 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacattat
      781 acagatactt ttataacatt caaggaaatt atgagaactg tttctaacat ataccacaat
      841 tgtgtaccag ataagattaa aaaccgaaga aatactgatc aactgaag
//
LOCUS       DASJTM010000268          870 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJTM010000268 DASJTM010000000
VERSION     DASJTM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.279"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..>870
                     /gene="bsh"
                     /locus_tag="U4B29_003116"
     CDS             4..>870
                     /gene="bsh"
                     /locus_tag="U4B29_003116"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2107986.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTY"
BASE COUNT          292 a          118 c          159 g          301 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat
//
LOCUS       DASJTM010000269          833 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJTM010000269 DASJTM010000000
VERSION     DASJTM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.282"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..372
                     /locus_tag="U4B29_003117"
     CDS             <1..372
                     /locus_tag="U4B29_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN2107987.1"
                     /translation="NDVFSPAFNDFLGVSSYPKVDLVENEKEYKLTADMPGCDKEDTT
                     VEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPNVDEEKITGTFKNGV
                     LKLVLPKTAYQPKETKKIELN"
BASE COUNT          277 a          126 c          143 g          287 t
ORIGIN      
        1 aatgacgtgt ttagtccagc attcaatgat tttttaggtg tttcaagtta tccaaaagta
       61 gatttagtcg aaaatgaaaa agaatacaaa ctaactgctg atatgcctgg ctgtgacaaa
      121 gaagacacga cagttgaata ttcagataat actttgacga tctccgccaa tcatgagtcc
      181 catacagaag ataaagaaga tggcaactat gtacggaaag aaagacattc ggtctcttac
      241 aaacgttcat tctatcttcc taatgttgat gaagaaaaaa tcactggaac ctttaaaaac
      301 ggtgtgctga aattggtatt acctaaaaca gcttatcagc caaaagaaac aaaaaaaatt
      361 gagttaaatt agaacttatc gcctctcgat gaatgagggg cgatttttat atatttggat
      421 aagaacgagt atgtttaaat tttcaaaatg agcaaaaaaa tattttgttt ttacgataaa
      481 gatgttatga tctctcttgg taaaaaatat gggagttgat catttgattg tcttaagtaa
      541 acttttgttt tggataccat ttatcataat gaccagtgct ttcttttact tattcaaaag
      601 gcctaacaaa tacttactct tatctgcttg tccgatgatc tattttatcc ataagattag
      661 aggattttat tattacgact ctggtatctc attgttttgc tacgaaatcg gcttattagc
      721 ttcggccatc tttttcatat ttgtgttaga atatttcaga aaaaaataaa cttccagtca
      781 gttttggctg gaagtttttt atttggtgca gatcttttca gctgttattc ata
//
LOCUS       DASJTM010000270          822 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJTM010000270 DASJTM010000000
VERSION     DASJTM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.283"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..153)
                     /locus_tag="U4B29_003118"
     CDS             complement(<1..153)
                     /locus_tag="U4B29_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2107988.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            234..809
                     /locus_tag="U4B29_003119"
     CDS             234..809
                     /locus_tag="U4B29_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2107989.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DASJTM010000271          816 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.284, whole genome shotgun sequence.
ACCESSION   DASJTM010000271 DASJTM010000000
VERSION     DASJTM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.284"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>816)
                     /locus_tag="U4B29_003120"
     rRNA            complement(<1..>816)
                     /locus_tag="U4B29_003120"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          259 c          181 g          211 t
ORIGIN      
        1 ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
       61 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      121 tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg cggaaaccct
      181 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
      241 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      301 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      361 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      421 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      481 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      541 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      601 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      661 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      721 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      781 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DASJTM010000272          803 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJTM010000272 DASJTM010000000
VERSION     DASJTM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.285"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(508..732)
                     /locus_tag="U4B29_003121"
     CDS             complement(508..732)
                     /locus_tag="U4B29_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107990.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          286 a          114 c          143 g          260 t
ORIGIN      
        1 gaaactctat ttacttcttt tttaatttgt tcttgcaatc ttttttgcta cgtaattttt
       61 gtaaaaaagt gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga
      121 aaaatattta ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa
      181 ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa
      241 gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc
      301 aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca
      361 ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt
      421 cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac
      481 agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa
      541 aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat
      601 aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag
      661 ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt
      721 atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat
      781 aaaaactgga aggaactgta cgt
//
LOCUS       DASJTM010000273          801 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJTM010000273 DASJTM010000000
VERSION     DASJTM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.286"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(227..498)
                     /locus_tag="U4B29_003122"
                     /pseudo
     CDS             complement(227..498)
                     /locus_tag="U4B29_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            609..>801
                     /locus_tag="U4B29_003123"
     CDS             609..>801
                     /locus_tag="U4B29_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2107991.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGY"
BASE COUNT          269 a          136 c          123 g          273 t
ORIGIN      
        1 attttgaaat aagactctat tattacaagt tcatccgttg taagatgggt ataggtcatt
       61 tgtactcact ccttataatt ctttggtcgg aactattttg agtgtagcac aaatgacttt
      121 tttagttgtc tagcttaatt ttacaatcgg cgatggcaaa aatttcaatc tttaaatggc
      181 gaaaaacaaa gagcgtgaga ccctatcctt atctcacgct ctttgtctat ttttctaaca
      241 aatcaagtat ttcttgagga gaatcaataa ttatatctac actttgaaaa ttttctatat
      301 ttttggctcc ccatttagct aaaccaaatg ctatcccaca agctttcgca ctttgcatgt
      361 catataatga atcaccaata taaatcgttt cttatttttt atgcccaatt catcaattgc
      421 cttttgtatg gggtccggaa aaggtttatg caacttagta tctgaagctg taatttgtat
      481 atcaaaatat agatttaacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa
      541 acatgttggt aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag
      601 gactacatat gcgttcatca aaatatactg aacattatac agatactttt ataacattca
      661 aggaaattat gagaactgtt tctaacatat accacaattg tgtaccagat aagattaaaa
      721 accgaagaaa tactgatcaa ctgaagcaac gcgatacagt gattattgct tgtgttatat
      781 ggggcataat taatggctat a
//
LOCUS       DASJTM010000274          792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJTM010000274 DASJTM010000000
VERSION     DASJTM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.288"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>792
                     /locus_tag="U4B29_003124"
     CDS             10..>792
                     /locus_tag="U4B29_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2107992.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT          269 a          137 c          153 g          233 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata at
//
LOCUS       DASJTM010000275          762 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJTM010000275 DASJTM010000000
VERSION     DASJTM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.289"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            30..503
                     /locus_tag="U4B29_003125"
     CDS             30..503
                     /locus_tag="U4B29_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN2107993.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            512..739
                     /locus_tag="U4B29_003126"
     CDS             512..739
                     /locus_tag="U4B29_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107994.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          257 a          120 c          139 g          246 t
ORIGIN      
        1 tagctatcaa agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg
       61 tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg
      121 ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt
      181 actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa
      241 tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa
      301 ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg
      361 atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg
      421 cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga
      481 cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt
      541 attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc
      601 aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa
      661 aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa
      721 tactatacca gaagaataaa ataaagtaga acaaacagta aa
//
LOCUS       DASJTM010000276          745 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJTM010000276 DASJTM010000000
VERSION     DASJTM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.290"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..657
                     /locus_tag="U4B29_003127"
     CDS             19..657
                     /locus_tag="U4B29_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2107995.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          231 a          119 c          123 g          272 t
ORIGIN      
        1 atagtaggag ttattatcat gaataacatt ttaggaaaaa aaatagaact taaaaaaggg
       61 atattgggca ctattgtcgt tgtactcggt ttgttagggt tgctatttgc gccatccatt
      121 attagcattt tttctttaac tatcactgga tttatagcat ttacacttgt ttttggtaaa
      181 aaagatacga aaaaaatgtt ctcgaaacct gtcgctcctg tcaaaaatat agccaaatat
      241 ttttttatca atgtagtaat cagtgccgca gtctcggtat ttttgcaaca aattttgaaa
      301 tggggtttaa caggcaatcc aataaatgag gctttcagcc cgttattatt tattattttg
      361 cctatcatga tacttggaga agaattgttc tcagtgtatt ttctcgctat tttttctagt
      421 aagttcagca ttcctgtcgc tagtttcttg tcagcaatca tctttggatt tatccattac
      481 agtacttatg ataatggaaa tattttacac actgtagcac acatcctttt gatacaaggg
      541 gtggcaagat tgttattcaa ccaagctgcc attaaaagca actcgatcat tacaagttgg
      601 gcagttcatg tgatatttga tttcacagct atattgctag tcgttttatt ctcctaaatt
      661 tttatagaaa taatacatgc tctattgcta agaaagcata ctttatttcc tttaaacaaa
      721 aagacagatg tatcaatgga ttaaa
//
LOCUS       DASJTM010000277          731 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJTM010000277 DASJTM010000000
VERSION     DASJTM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.291"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            370..>731
                     /locus_tag="U4B29_003128"
     CDS             370..>731
                     /locus_tag="U4B29_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2107996.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSV"
BASE COUNT          273 a           97 c          120 g          241 t
ORIGIN      
        1 gattttaggg tggacataaa gcgatgcgaa catggacaaa aagcgatgtg aatgtggaca
       61 aaaagcgatg caaacgtgga caaaaagcga tgtggtattc cgaatatatc caaaaaaaga
      121 aaaagtatgt gaatttattt ctaaaaacta gaaaaatatt tgttttttaa ttttggtaat
      181 ttttataatt tcgtatttta ttgaaaagga tttttatttt gaaaatgttc taaaatagcg
      241 atttctgctg tattttcaat gaaatttgat tggaaaaaat aaaatgtgtt ttactgacat
      301 tgagttatgt tataaattta cgatacattt cttattgtaa cgtatcgaaa acaggtctat
      361 acttattcaa tggaatcgta ccagttccaa gtttttaagc tcgtaccaag cttagaaaga
      421 agaggcaatc gtatgaaaat aacagaaaag aatgtaaacc cgaaatttga aactaccgaa
      481 agcaatctaa aaaagtttga tttaactgtt actttatcga atcaacttgt agatagcatt
      541 gttgaaatgg atgctatgcc acaaaaaatt tatctttacg ctataggaca tcttgaccct
      601 aaaaatccgc cttttaataa taccgtcaaa gtatctaaaa aggctttatt tgcgttgatg
      661 tcttctagtt catcttcttc aaaacattca agattaaaac aagttattga aaaattgcat
      721 cagcaatctg t
//
LOCUS       DASJTM010000278          727 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJTM010000278 DASJTM010000000
VERSION     DASJTM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.292"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..227
                     /locus_tag="U4B29_003129"
     CDS             54..227
                     /locus_tag="U4B29_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107997.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          245 a          121 c           96 g          265 t
ORIGIN      
        1 tattcacttc tttggtttat actattctta taaataaaaa cgaggtatgt cctatgatga
       61 aaagattgta ctattcattg atcataacga ttggctattt gattgtatca aatctaggaa
      121 atatggtatt tggtatcagt aaagaattca gttggactac tacattatgg gaatctctat
      181 tctttttcat cttcgtattt ttgttacaaa attatcgaaa aaaataaaca tgtgccatct
      241 atctgttggc acatgtttta ggatttatgg tactttttat tcggcaatcc attttatttt
      301 tctacttctt cttaatacct ctaacacttt tgatttataa actaatccaa tgcatctact
      361 tgcgcttatt aagctatttc agctagatag taccacccca tgcgtcaatg cttgattttc
      421 cttttgtata acattgtata agcaataaag gacaaataag tggtgattac accaaaccat
      481 tgaataagaa aaattctgat acgtaaataa acatgaactt gaaaaaatta atgaatcggt
      541 tagctactac gtagttgtct tttttctcat acagttctgt taacttacac ttctcttcgc
      601 tagataataa aaaatcctcc aactgtattg tccaatataa tcccaggcat ctaaactagc
      661 attatagaag agacacacat agaatctaaa ataactggtt ttaaatgttt cttacaatca
      721 aaaaaag
//
LOCUS       DASJTM010000279          724 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJTM010000279 DASJTM010000000
VERSION     DASJTM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.293"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..222
                     /locus_tag="U4B29_003130"
     CDS             37..222
                     /locus_tag="U4B29_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2107998.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            235..483
                     /locus_tag="U4B29_003131"
     CDS             235..483
                     /locus_tag="U4B29_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2107999.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            514..684
                     /locus_tag="U4B29_003132"
     CDS             514..684
                     /locus_tag="U4B29_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108000.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT          265 a          100 c          159 g          200 t
ORIGIN      
        1 gcttacaaat tatttgttta gaaaggagct atttttatgt ctgagaattt agacttaaaa
       61 attcgagcgg agatgagaaa aagaagaatg actttcaaag aactagctgc gcttatcggt
      121 atttcaggag cttatttatc agatattcta aacggcaatc gtgatggaaa gaaagcacaa
      181 cagcatatcg aaacagtgaa aaaaatattg gacatccgat agggggggta ggcgatgaca
      241 aaactaaaaa aacaagattt tgtaaaaaaa tataattatt ctccatctac ttatcaacgt
      301 cgaatgtcgg aactaaaaaa tacagcaatt ttctcagcgg cgtatgaacg ggtcacagga
      361 caagaagttt ggatcaatac agaattatac gataaatttt tgtctttcaa atcctataac
      421 aggttacgca caagaaaggt aacacctaaa gaatttatcg agaagcattt agttgattta
      481 taaaaaacag aacattttga gaggtgaagg ttaatgggta aattcaacag agcattagta
      541 ttcagcgcac cgctaatcgt ctatgcttta gggctttggg gaagtagaca agcgttgata
      601 ggaacgattg tgtacatggt ttggattttt atagggctgg atgaagctga gtacaaaaca
      661 aaaaagccag tcgggaggga ctgactaatg agtaaggacg atattgtatt tctgttaatt
      721 atgt
//
LOCUS       DASJTM010000280          724 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJTM010000280 DASJTM010000000
VERSION     DASJTM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.294"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..325)
                     /locus_tag="U4B29_003133"
     CDS             complement(<1..325)
                     /locus_tag="U4B29_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108001.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKI"
     gene            complement(332..>724)
                     /locus_tag="U4B29_003134"
     CDS             complement(332..>724)
                     /locus_tag="U4B29_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2108002.1"
                     /translation="FDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIV
                     PRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQ
                     LGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          237 a          152 c           81 g          254 t
ORIGIN      
        1 taatttttaa acaaaattct ccccaaaaag gaactttttc tagtgaaaac ttggtaatct
       61 ctttgtaatt aattcttgtt aaagaataat taaacacttc atttttactt gttatttttt
      121 cattgactat aactagttca tctggtaaaa aaattaacca acaaacatcg acaaacagta
      181 aatgatataa tccatttcca cctccaagca tattcaatac tggtgaacat gccttagaaa
      241 cacgaatata gttatggttt aaatagatat tttctttaaa cattgcttgt ttaaaagcca
      301 tatgctcttg ttctaaactt ttcataaact tttactctaa tgcacagcta gcagcagctg
      361 ctgcaattcc tgctacaata tttaccttag ctaactcttt agctgcagct tttcctaatt
      421 gtttttctgc aatttttaat aataaatccg cagctttttc aaaattaaaa gcttttaatg
      481 cttcaataaa atctccctgc gtacctatag cccaagccac tcctcctcca ggaagagaat
      541 taatggctac acatccagca tatcgtttcc aatcaacttt ctccattcta ggaacaataa
      601 tatgttcata aaagactctt gctgctcttc cttgttcgtt atcttgactg attcgttcac
      661 gaatagcacc aaaatccttt atatggtata caccatgttc gtctaaaaaa ccaactgtat
      721 caaa
//
LOCUS       DASJTM010000281          721 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJTM010000281 DASJTM010000000
VERSION     DASJTM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.295"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(314..514)
                     /locus_tag="U4B29_003135"
     CDS             complement(314..514)
                     /locus_tag="U4B29_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2108003.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          268 a          113 c          111 g          229 t
ORIGIN      
        1 tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata aattcttcca
       61 tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa aggtggaatt
      121 tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc aaaaaaaatc
      181 gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct tataataaca
      241 aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc tgtatccttt
      301 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
      361 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
      421 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
      481 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
      541 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
      601 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
      661 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
      721 a
//
LOCUS       DASJTM010000282          691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJTM010000282 DASJTM010000000
VERSION     DASJTM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.296"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..447)
                     /locus_tag="U4B29_003136"
     CDS             complement(<1..447)
                     /locus_tag="U4B29_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2108004.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          207 a          119 c           92 g          273 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat a
//
LOCUS       DASJTM010000283          683 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJTM010000283 DASJTM010000000
VERSION     DASJTM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.297"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="U4B29_003137"
     CDS             132..344
                     /locus_tag="U4B29_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108005.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>683
                     /locus_tag="U4B29_003138"
     CDS             506..>683
                     /locus_tag="U4B29_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN2108006.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSR"
BASE COUNT          245 a          102 c          120 g          216 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggt
//
LOCUS       DASJTM010000284          667 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJTM010000284 DASJTM010000000
VERSION     DASJTM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.298"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          208 a          102 c          104 g          253 t
ORIGIN      
        1 gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta
       61 gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac
      121 tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta
      181 gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga
      241 tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt
      301 tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta
      361 cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt
      421 tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact
      481 tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg
      541 acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata
      601 cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt
      661 aatttcg
//
LOCUS       DASJTM010000285          662 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJTM010000285 DASJTM010000000
VERSION     DASJTM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.299"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(180..482)
                     /locus_tag="U4B29_003139"
     CDS             complement(180..482)
                     /locus_tag="U4B29_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108007.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          235 a          107 c           88 g          232 t
ORIGIN      
        1 aaaaagactt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
       61 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
      121 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
      181 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      241 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      301 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      361 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      421 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      481 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      541 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      601 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      661 aa
//
LOCUS       DASJTM010000286          661 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJTM010000286 DASJTM010000000
VERSION     DASJTM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.300"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="U4B29_003140"
                     /pseudo
     CDS             172..459
                     /locus_tag="U4B29_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..>661
                     /locus_tag="U4B29_003141"
     CDS             473..>661
                     /locus_tag="U4B29_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108008.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAV"
BASE COUNT          235 a           99 c          143 g          184 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 t
//
LOCUS       DASJTM010000287          659 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJTM010000287 DASJTM010000000
VERSION     DASJTM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.301"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..>659)
                     /locus_tag="U4B29_003142"
     CDS             complement(3..>659)
                     /locus_tag="U4B29_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2108009.1"
                     /translation="NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYP
                     RINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE
                     "
BASE COUNT          215 a           96 c           74 g          274 t
ORIGIN      
        1 ttctattcat ctatatttcc aactttcaaa atttctttat caattctatt taatagttta
       61 tcagagtaat ccgatttaac ttttcttaca aataattttt cactttcttt tatttgatct
      121 atatcccttt catcaaacca tttgttcatg gaaggatcaa taatatgaat atttgctaat
      181 ttcgaagttt gtaaagtagt ttcttcaata tttttcaatt caagtggtaa atctggtcct
      241 ccaataacat ttttattttt gaacgaggaa ttaaaaaaaa gtgtatgaaa aaatttttca
      301 tctggggcaa acatacctat tttaaaattc aggtagtctt gttgattttg ttcatacatt
      361 tgtattaatt ctgttaggca ttctttcgac aaagcccacc attgagaacc atgatacaaa
      421 tcaaatttat ggtgtctaat ttttggggga agtttaatca tcctcagaca acgattaatt
      481 atagctctaa aaattttaaa tgcagttgtg ttattcctat taattctagg ataatcatgt
      541 ttttgatata catctatatg tttataataa aattctttat gtttaatttg atctagatct
      601 attccacgaa taaactccac tgaactattc tttttaaaat aattataaat atactcatt
//
LOCUS       DASJTM010000288          656 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJTM010000288 DASJTM010000000
VERSION     DASJTM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.302"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U4B29_003143"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U4B29_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN2108010.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="U4B29_003144"
     CDS             complement(481..>656)
                     /locus_tag="U4B29_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108011.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DASJTM010000289          646 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJTM010000289 DASJTM010000000
VERSION     DASJTM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.303"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            221..619
                     /gene="spxA"
                     /locus_tag="U4B29_003145"
     CDS             221..619
                     /gene="spxA"
                     /locus_tag="U4B29_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2108012.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          230 a           96 c          122 g          198 t
ORIGIN      
        1 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
       61 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
      121 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
      181 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
      241 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
      301 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
      361 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
      421 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
      481 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
      541 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
      601 attgatggct ggattataga aggataatac gaaacttgga aaagac
//
LOCUS       DASJTM010000290          616 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJTM010000290 DASJTM010000000
VERSION     DASJTM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.304"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..146
                     /locus_tag="U4B29_003146"
     CDS             <1..146
                     /locus_tag="U4B29_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2108013.1"
                     /translation="IVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIA
                     EEK"
     gene            147..>616
                     /locus_tag="U4B29_003147"
     CDS             147..>616
                     /locus_tag="U4B29_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2108014.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKR"
BASE COUNT          234 a           74 c          115 g          193 t
ORIGIN      
        1 acattgttaa agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt
       61 tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag
      121 tattgaaaat agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg
      181 ggtctaaggg gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt
      241 tcgctttagc aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt
      301 gtttgaatac ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga
      361 aaattgtaga acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac
      421 gtgattcatt cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag
      481 atttgacttc acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa
      541 aaaatttaga taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta
      601 atatggtaaa aagaat
//
LOCUS       DASJTM010000291          605 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJTM010000291 DASJTM010000000
VERSION     DASJTM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.305"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            236..>605
                     /locus_tag="U4B29_003148"
     CDS             236..>605
                     /locus_tag="U4B29_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108015.1"
                     /translation="MELLEKAYSSTELIDSYEYEDLKYIKDQRNYSAHPVVTYNGNTW
                     ELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALK
                     NSYLNKLSEKAKENLCKTAFK"
BASE COUNT          252 a           82 c           95 g          176 t
ORIGIN      
        1 tgaaataaaa aaagttaaaa atcctagaat tattccatta ttagaagaag ttagaagtag
       61 tttttatcag ggaaattatc gttctgcaat agctgtcaat tacagtgcgc ttattttaga
      121 tttaattgat aaattatctg atttagcaaa tatttacgag gataatactt ctaagaaaat
      181 tttagaagat atagctagac taagaaaaag tgatccaaac tctccaaaat gggaaatgga
      241 actactagaa aaagcttata gttcaactga gctaattgat agctatgaat atgaggattt
      301 aaaatatatt aaggatcaaa gaaattattc agctcatcct gtagtcactt acaacggtaa
      361 tacttgggaa ttaagagata taacaaaaga aacttgtcag gatgttatac ggaagtccta
      421 tgaaattata ttcctaaaaa atccaatatt aggaaaaaaa gtaacgatcg aaattataca
      481 atatgcaagt cgcatacatg ctatgtctgt tacacctgaa gaatttcact ctgcactaaa
      541 aaatagctat ctaaataaac taagcgaaaa agccaaagaa aacctatgta agacagcctt
      601 taagt
//
LOCUS       DASJTM010000292          589 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJTM010000292 DASJTM010000000
VERSION     DASJTM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.306"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(129..359)
                     /locus_tag="U4B29_003149"
     CDS             complement(129..359)
                     /locus_tag="U4B29_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108016.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          206 a           90 c           69 g          224 t
ORIGIN      
        1 aattaataag ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa
       61 atctttgaca aatctattcg aaggatcaaa tttaaaccat aattgcgtct cattaaaaag
      121 cataaccatt actccgatct atttttaaac ttagtctcta cctctaatat atatcgagtt
      181 tttattttgc cttcagagaa tactgtttct ttttttgcag ttacaggttg tttattttcg
      241 gaaaaagcta atatagctaa aattgaaaca tccatctgga atttatcttt tttactgctt
      301 tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc ttccatcata
      361 aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga tatttgaggg
      421 gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg aatgacatac
      481 aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt cttttatata
      541 tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaata
//
LOCUS       DASJTM010000293          589 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJTM010000293 DASJTM010000000
VERSION     DASJTM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.307"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          237 a           66 c           77 g          209 t
ORIGIN      
        1 ctatttaaat tttcctgcta ataaaataac gaaaatagaa tatttagctt ttttatacca
       61 ttttacgata acatgatttc atttgtaata ttaaaacatt taaattttta attattcttt
      121 caaattagat caattaatat ccttataaaa gagcataata aatggttttt acatcgttat
      181 ttattgatat taatctcatc caaaaaaaat gaaaacgcat acttttatgg gtgattgaat
      241 gtgattttaa tacatatacc aattaaaaga aaacttttta gatttttact tacaaattta
      301 taaaaaaaca agataattga tactttgatt ttgcgaacat tttatcttgc cgcctaattc
      361 agctttaata tcatgattat atcagtaaca aatcgctttt ttagctaatt taagcatcaa
      421 ataattattc ttaatcaggg aaaaaataac taataaatgg attttgggaa taggtaaaaa
      481 cttgatcggt ttctggctaa aaataacgtg aaaaaggaaa tataaaataa catggagcgt
      541 gggaggaata aagaaatttt acacgaaagg tgcgcaggga tgtaaagaa
//
LOCUS       DASJTM010000294          580 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJTM010000294 DASJTM010000000
VERSION     DASJTM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.308"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>580)
                     /locus_tag="U4B29_003150"
     CDS             complement(<1..>580)
                     /locus_tag="U4B29_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN2108017.1"
                     /translation="VSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKT
                     PLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKV
                     DLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNY
                     AFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI"
BASE COUNT          216 a          104 c           71 g          189 t
ORIGIN      
        1 aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt catctataaa
       61 aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa
      121 aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc
      181 aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct
      241 ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct
      301 attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag
      361 cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg
      421 taatattact tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc
      481 tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc
      541 aaaagaaaaa tataccaata gaactaataa tatgcttacc
//
LOCUS       DASJTM010000295          578 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJTM010000295 DASJTM010000000
VERSION     DASJTM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.309"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..381
                     /locus_tag="U4B29_003151"
     CDS             46..381
                     /locus_tag="U4B29_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108018.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          224 a           67 c          104 g          183 t
ORIGIN      
        1 acaatttcct aggaaaaata gttaacataa aaggaatgaa ttgttatgaa acaatattta
       61 aatgaatgga aagcgataga ggggtcatta gtaacgcaaa gaataaagga attacctgat
      121 tgcttagaga aggatcattt gtttcaaata cgagaaatgt tgaaaaaaga acagtttgat
      181 ccagaccaat ttttagtagt cgagtatcca actattggag tgtactgttg taaccatatc
      241 aatgatggaa aatattttat tattcaggag tatggaggac agttaactcc tttctatact
      301 acatgggaaa tgagtgaaga aggaatcaat aattttccat gtgaaagcat cgaagattca
      361 atttcacaca ccgaatgtta attgattata actataaaaa aggattgaac attatgttca
      421 atcctttttt caatgaaatg ttttccgcca tttcattgtt tggtgcgatt tctattttat
      481 tatatcaaaa taaagagaga ttctaaagaa agaagaggga aatatataaa tcaagcgaca
      541 gtatatttat tattaaatgc tattgtgaga atataact
//
LOCUS       DASJTM010000296          572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJTM010000296 DASJTM010000000
VERSION     DASJTM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.310"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..267
                     /locus_tag="U4B29_003152"
     CDS             37..267
                     /locus_tag="U4B29_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2108019.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            267..>572
                     /locus_tag="U4B29_003153"
     CDS             267..>572
                     /locus_tag="U4B29_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2108020.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     "
BASE COUNT          216 a           80 c          109 g          167 t
ORIGIN      
        1 gttatatagt atacttttat taagggagtg atgataatgc ttgttacaaa atctagatta
       61 caaggaagtt ctgtagtggt aactttacct tctgataacg gaaagaaacc ttcagaaaac
      121 caagaatata tcgttgtgta ttctgatgat ggaacaatta cgttagttcc aaaaattgaa
      181 gatccattta gtggtgggga agaggccgag tattatgaga aagacgaatg ggaagattta
      241 actcctgaag ggagagaaat tttatagtgt cagaagaaag aatttatatt cctaaaaaag
      301 gtgatattgt atggattgat tttgatccct ccgtaggaaa agaaattcaa aaaagacgac
      361 caggactagt cgtctctcgt tatgaattta accgtaaaac aatgttttcg gtcatatgtc
      421 caattacttc cactataaag aatttaccga cacgttactc attacctgaa gagctagata
      481 taaaaggaca agtaattatt acacaattaa aatctcttga ttttaaggaa cgaaaactca
      541 aaaaaataga aaacttacca ttgcaagaca tg
//
LOCUS       DASJTM010000297          555 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJTM010000297 DASJTM010000000
VERSION     DASJTM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.311"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..85)
                     /locus_tag="U4B29_003154"
     tRNA            complement(13..85)
                     /locus_tag="U4B29_003154"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:His,seq:gtg)
     gene            complement(147..231)
                     /locus_tag="U4B29_003155"
     tRNA            complement(147..231)
                     /locus_tag="U4B29_003155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(195..197),aa:Leu,seq:caa)
     gene            complement(252..322)
                     /locus_tag="U4B29_003156"
     tRNA            complement(252..322)
                     /locus_tag="U4B29_003156"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(288..290),aa:Cys,seq:gca)
     gene            complement(368..439)
                     /locus_tag="U4B29_003157"
     tRNA            complement(368..439)
                     /locus_tag="U4B29_003157"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Gln,seq:ttg)
     gene            complement(451..523)
                     /locus_tag="U4B29_003158"
     tRNA            complement(451..523)
                     /locus_tag="U4B29_003158"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:His,seq:gtg)
BASE COUNT          168 a          141 c          127 g          119 t
ORIGIN      
        1 ccaagaataa aaggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
       61 gcgttaacca cttcgccaca ttcgccatca taaaagatgt tatttaattt ctcaataaaa
      121 aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg cacgccctca
      181 acgggcactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caagttgatt
      241 tattagtact aaggcggtaa ccggatttga accggtgatg aaggttttgc agacctctgc
      301 cttaccactt ggctataccg ccattatact attatcaatc tataaaataa agaataaatt
      361 atagaaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
      421 accgcttggc tataccccaa gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg
      481 ccggagccac aatccggtgc gttaaccact tcgccacatt cgccatggca ggggcagcag
      541 gaattgaacc cacac
//
LOCUS       DASJTM010000298          539 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJTM010000298 DASJTM010000000
VERSION     DASJTM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.312"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            97..291
                     /locus_tag="U4B29_003159"
     CDS             97..291
                     /locus_tag="U4B29_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108021.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          226 a           67 c           87 g          159 t
ORIGIN      
        1 attcttttta tttatcataa agatatagat acataatcat tttcacttac atatatttga
       61 atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga agtaaaaaca
      121 aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc tttacttggt
      181 tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa gaacgagcta
      241 gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta aatccaatgt
      301 atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta tagaattaga
      361 agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc atatgattat
      421 ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga atcaaatata
      481 cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat aatgtatca
//
LOCUS       DASJTM010000299          524 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJTM010000299 DASJTM010000000
VERSION     DASJTM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.313"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(139..>524)
                     /locus_tag="U4B29_003160"
     CDS             complement(139..>524)
                     /locus_tag="U4B29_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEN2108022.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          166 a           80 c           88 g          190 t
ORIGIN      
        1 ctacaaacta cttttcaata agtaggaatt atagaaattt tactcaaaaa atcttcagta
       61 aacaatttgg ctaaataaga ttcagttgtt atacgagaag gttacggttc ttcttaacta
      121 agcttagtat gtttttattc aaggcatgat tttctctatg tgatcattca gtttctgtaa
      181 gagttctaat aattcttttt tggagtactt gtaattgctt tctacccacc gattgagaac
      241 ggatagacaa ccttggacgt ggtacgtagt aaagaatctc atttcttctg tgataatcgt
      301 tttcttgtct tcatatagcc atatgttcag atagttttct tcaataactt gtttaacttt
      361 cttctgaaac gaaatatcac catggttgag gataaataag cgatataaaa aagtatttgc
      421 gtaaacatag tcaatcaagc gagtgtatag ttcctcatag ctatgggctg gatcataaga
      481 gagaatttca tttagatccc gaaaaaagtt gctttctaat tgat
//
LOCUS       DASJTM010000300          512 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJTM010000300 DASJTM010000000
VERSION     DASJTM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.314"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..343
                     /locus_tag="U4B29_003161"
     CDS             <1..343
                     /locus_tag="U4B29_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2108023.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DASJTM010000301          497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJTM010000301 DASJTM010000000
VERSION     DASJTM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.315"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            251..400
                     /locus_tag="U4B29_003162"
     CDS             251..400
                     /locus_tag="U4B29_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108024.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          193 a           71 c           88 g          145 t
ORIGIN      
        1 tattgctaag ttatttataa agaggtgagc atatgattta tattcaagca atagtatttg
       61 taaccttgat gattgggcta cttatcggat taaaagaaaa tagctaaacc atttaagaaa
      121 aaataaataa ggaaaatgat aaatgctttt taattgcatc aaaaaacgta cgatgctaaa
      181 cttggtataa gttcgaaagt ttaaaggaaa ctttcaagaa ctaaattaca ttacaaagga
      241 gatgaatttt atgacgttta caaaaacaaa tatgcaatac cgcgtagctt ttgatacaac
      301 ttctaaccgt tttatggcga ttgatgcaca aaacgaaaat aacgttgcgt ttggtattac
      361 aatcgaacaa gcagtccgag cattaaacga atctagataa ggagtgaacc attccaaatg
      421 gcgactgatg agatctcgga aaatcaataa aagacgtttg taacggtaat ccgcgacaaa
      481 cgtctttttg tattcat
//
LOCUS       DASJTM010000302          489 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJTM010000302 DASJTM010000000
VERSION     DASJTM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.316"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..388
                     /locus_tag="U4B29_003163"
     CDS             149..388
                     /locus_tag="U4B29_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2108025.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            437..>489
                     /locus_tag="U4B29_003164"
     CDS             437..>489
                     /locus_tag="U4B29_003164"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2108026.1"
                     /translation="MLVFQMRNVDKTSTVLK"
BASE COUNT          204 a           58 c           93 g          134 t
ORIGIN      
        1 tctttctcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat
      121 aagaataaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga
      181 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      241 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      301 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      361 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      421 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      481 tttgaaaca
//
LOCUS       DASJTM010000303          487 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJTM010000303 DASJTM010000000
VERSION     DASJTM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.317"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>487)
                     /locus_tag="U4B29_003165"
     CDS             complement(<1..>487)
                     /locus_tag="U4B29_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEN2108027.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DASJTM010000304          487 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJTM010000304 DASJTM010000000
VERSION     DASJTM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.318"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            135..>487
                     /locus_tag="U4B29_003166"
     CDS             135..>487
                     /locus_tag="U4B29_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2108028.1"
                     /translation="MEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGD
                     GKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGG
                     YPSELKTDAEKRAWR"
BASE COUNT          179 a           61 c          105 g          142 t
ORIGIN      
        1 tacaaactgg attaatcaaa gcagatatct ttcaaaattc ttttaacaag actggagatg
       61 tattgaaatt agttaatgga ctactccaaa tttggaacaa caaaaagaaa ataatggagt
      121 taactaaaaa aggaatggaa ttttggaatt ctaagggttc aattggaacg attggaacaa
      181 ctgattctgt tggtaatcct tttcctgggg cttctactcc aacccctatt gaagataatt
      241 ctttagttat tcgtacaaat ggagacggca aatatatttt gatttctcct aaagtaggaa
      301 aaggattagt tttattagga aatggtaaag caatttattt tggagactta gatgtacaag
      361 gaaaacttac agttaaagga aaagaaataa ttcctggaca aggtggtggt ccatcgggag
      421 gtggagaatc tactggtggg tatcctagtg aattaaaaac agatgcagaa aagagagctt
      481 ggagaat
//
LOCUS       DASJTM010000305          483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJTM010000305 DASJTM010000000
VERSION     DASJTM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.denovo.319"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            38..127
                     /locus_tag="U4B29_003167"
     tRNA            38..127
                     /locus_tag="U4B29_003167"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="U4B29_003168"
     tRNA            142..213
                     /locus_tag="U4B29_003168"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="U4B29_003169"
     tRNA            229..301
                     /locus_tag="U4B29_003169"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="U4B29_003170"
     tRNA            311..383
                     /locus_tag="U4B29_003170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
BASE COUNT          109 a          107 c          132 g          135 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtc
//
LOCUS       DASJTM010000306         9065 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.104, whole genome shotgun sequence.
ACCESSION   DASJTM010000306 DASJTM010000000
VERSION     DASJTM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.104"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..634)
                     /locus_tag="U4B29_003171"
     CDS             complement(<1..634)
                     /locus_tag="U4B29_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2108029.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGK"
     gene            1146..1781
                     /locus_tag="U4B29_003172"
     CDS             1146..1781
                     /locus_tag="U4B29_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2108030.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1935..2012
                     /locus_tag="U4B29_003173"
                     /pseudo
     CDS             1935..2012
                     /locus_tag="U4B29_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2257..2373
                     /locus_tag="U4B29_003174"
     CDS             2257..2373
                     /locus_tag="U4B29_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN2108031.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2389..4308
                     /gene="tet(M)"
                     /locus_tag="U4B29_003175"
     CDS             2389..4308
                     /gene="tet(M)"
                     /locus_tag="U4B29_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN2108032.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4427..4594
                     /locus_tag="U4B29_003176"
     CDS             4427..4594
                     /locus_tag="U4B29_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN2108033.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(4654..5007)
                     /locus_tag="U4B29_003177"
     CDS             complement(4654..5007)
                     /locus_tag="U4B29_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2108034.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            5511..5930
                     /locus_tag="U4B29_003178"
     CDS             5511..5930
                     /locus_tag="U4B29_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2108035.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            5956..6162
                     /locus_tag="U4B29_003179"
     CDS             5956..6162
                     /locus_tag="U4B29_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2108036.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(6390..6563)
                     /locus_tag="U4B29_003180"
     CDS             complement(6390..6563)
                     /locus_tag="U4B29_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108037.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            6539..7204
                     /locus_tag="U4B29_003181"
     CDS             6539..7204
                     /locus_tag="U4B29_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2108038.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(7286..8452)
                     /gene="dnaJ"
                     /locus_tag="U4B29_003182"
     CDS             complement(7286..8452)
                     /gene="dnaJ"
                     /locus_tag="U4B29_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN2108039.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8603..>9065)
                     /locus_tag="U4B29_003183"
     CDS             complement(8603..>9065)
                     /locus_tag="U4B29_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEN2108040.1"
                     /translation="VSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKAR
                     KEEVDLRNDVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTK
                     RDALNEIVQNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
BASE COUNT         2829 a         1663 c         1697 g         2876 t
ORIGIN      
        1 tttttccgct tagttttcca ccctgcacat tttcataatt tttacagcga caatccgcac
       61 cgccatttct gtatttttca gacaaaacgg gaatatccaa cagaccacac atatcattga
      121 ttgccaagtc aaaacgtcgg ataacaccgt cacagtcaag acagcggttt aaaaatgaat
      181 accaatctct gttctgtgcc tgcaagacat attccatgtt gcgtgagcct gcccctttta
      241 attccaagaa tacgcccatg tgttcgtcat cagaagccat gaccttgatt tctccgtagg
      301 cgtaatgctc tttatagcca taatacccat agtcataatg gataaaataa tctgctttca
      361 tgccaagcac ctttcggata atttccaatg cgtcagtcgt tggataacgg acagacagat
      421 aatcaacgag cagataaaaa ggcttatcgc agatataata attcagacaa cgcaggataa
      481 tattttgtgt ttccttgctg gcgttggctt ttccgttctc aaagcggttg atactatatc
      541 ttgatacatg ggtaagactt gcaagttctc cttgtgaaag accgcttgca agacgtttgt
      601 tcttcatttc aagtgcaaat tctaaattgt tcattgtatt cctccaatcc cacaatagcc
      661 ttgaaatacc tgtaaacctc tattcaaaaa gtgtgatatg cactatcttc ttaaaacttc
      721 tcattactac tcacaacttt gggattgtat ggtttttgac ttgtcttttg tgaaatagtg
      781 cccccctgtt agataacggg ggcacagaca aggctcgcag gctcgccagc cgacaaagca
      841 tttcttcccc ttgacacaag gtgtcatggc ggtaagcctg agaggcttcc gccaccgtgt
      901 cagaaatact ttgtcagcaa gttctgtttt gccttttcta acaagttcca tttcaaccga
      961 tattcatatc tacggattta atcagatagg tatttctttt tgaatgtttt tatatttcat
     1021 tctattgtgc ttattcgatt acaagaacat agtaattctt atatgcttat ttgtcaatat
     1081 ctttttttgc ctttacaaat catattaagc atgatagact tataatagcg aagatataac
     1141 gagatatgga gggttatgca atgaaaataa gccatttagg aaataatata cagaccataa
     1201 gaaaatttag gggaatgaaa caacaggaac ttgcggacaa aatcggtatc aatatgcaga
     1261 gcctttccaa gattgaaaga gggttgaact atcctgccta tgaaacgctg gaaaagataa
     1321 tggaagtgct ggacgttacg cccaatgaat tgttgtcggg agaatggaag tatgtcaatc
     1381 aatctgaaaa ggaagtctgt cagtttttaa gagccgaaga acgtctaaat gcagaactga
     1441 aacatggaca ctatgataac ttctttgaca gcgaggaaga atggctggaa tatgagttag
     1501 aaaagttacg ggaatatatt acagactata tcaacgggaa aagcattgaa gcgtccgacc
     1561 tttacccaat caaagaattt atccagcatt tgaagtttca aaaattatta gaccgctatg
     1621 atgatttata cagtatggat atgtttgggg aaagcataga gggacataag tataagacac
     1681 cttatcaagt cgtgaaaatg ataaacccga actcaaaaga agatatggaa ctgttacgag
     1741 aagtattaag gaataacaat tttgatgatg aggacgagta acttttttcc tcttgcgaga
     1801 agatacagca aacaaaaagc ccgatcaaga gtgcaaagca ccaccaatgg tggcaaagct
     1861 cttgacaggg ctttttcttt tctgttgtgg gcaatcaaga ggaagaaagg gataaaatct
     1921 gatttgtggt aaatcaggca aaggcaacgc aggtatctca atttgatttg gtacttgaaa
     1981 agaacgggag taattggaag attatagaat aacaaatatt ggtacattat tacagctatt
     2041 ttgtaatcac gtactctctt tgataaaaaa ttggagattc ctttacaaat atgctcttac
     2101 gtgctattat ttaagtatct atttaaaagg agttaataaa tatgcggcaa ggtattctta
     2161 aataaactgt caatttgata gtgggaacaa ataattggat gtcctttttt aggagggctt
     2221 agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta tccggagaat
     2281 atctgtatgc tttgtatgcc tatggttatg cataaaaatc ccagtgataa aagtatttat
     2341 cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt
     2401 aatattggag ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta
     2461 tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat
     2521 aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag
     2581 tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa
     2641 gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc
     2701 gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc
     2761 ttttttatca ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt
     2821 aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg
     2881 tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac
     2941 cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa
     3001 agcataagat ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat
     3061 atagggattg ataaccttat agaagttatt actaataaat tttattcatc aacacatcga
     3121 ggtccgtctg aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt
     3181 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca
     3241 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag
     3301 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat
     3361 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
     3421 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
     3481 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
     3541 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
     3601 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
     3661 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
     3721 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
     3781 tctcttggat acttaaatca atcgtttcaa aatgcagtta tggaggggat acgctatggc
     3841 tgtgaacaag gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc
     3901 ttatactata gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg
     3961 gaacaagtct taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt
     4021 tatgcgccac aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac
     4081 atcgtagaca ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg
     4141 tgtattcaag aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta
     4201 acagagttaa aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca
     4261 aatagtcgga tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt
     4321 tgttatataa atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa
     4381 ttaaagtgta aaggaggaga tagttattat aaactacaag tggatattgt gtcctgtatg
     4441 tggaaataaa acacgattaa agataaggga agatactgaa ttaaaaaaat tccccctcta
     4501 ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag cagttcaaag taactgtgat
     4561 tacagagcca gacgcaaaga cgcagagccg ataaaatgag attaatacaa tctcatttta
     4621 tcggctcttt ccgttatgta tggattcttt taattagtct tcgatgtttc ttgcttcgtt
     4681 gataccgctg gctaaagatt ccattaagga tagttctttg tctgtaaagc tatccatgta
     4741 tttctctatc tgtaatcgtc gggtgctttt taccaagtta ttagcaggta agaaaaattc
     4801 atcaacggaa acatgaagta acgatacaag gtcataaaga acttgtatgc tggggtgttg
     4861 ccctttattt tcaatattag ttaagtaccg tgggtcaatt tcaatcaatg ctcccacttg
     4921 ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg gctaaaccaa aggctctaaa
     4981 atcatattta tcttcttttt tacgcatagt agaccacctc tatacatttt attgttccta
     5041 ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt tataggttta tatttaataa
     5101 aaagcactac taaacgccaa taaaaaaaac cgttatatgg tagtgctatt tacgctgtta
     5161 aaatattgta tattacttcc aaatggcggt ttgttggagg tcaacgtcgc catgaagtac
     5221 atcatataca ataaatttcc ttacattggg ttcttgtcaa aaaaagtcgt ctatctgcaa
     5281 tagataagta cgtccaccaa tgtggtttta taaatcatat agatagaata acagaagcat
     5341 gtaaacagag aaataaatct gtttatatgc ttttttggct attcagaact tttttacaaa
     5401 gtttatttat cagtaatgca acaaatcccc ctttcacatt gggactaaga gtgaaaggag
     5461 ataaacgagc aaggctcact tcctttccta gacagaaagg gggtgagatt atgaggacat
     5521 cttctttcga gcatatcgtc agattacagt tcaattcttt aatgctgatt gtcattaaga
     5581 acaaagtgaa aagtcgaaac agacagcttg caagacattc taaacgtgaa gtattgtttt
     5641 gtgaaatagc cgaaacgaaa caagttgaac gtggaacgat tgatacctgt ttttatgatt
     5701 gtgtatcatt taaagtcttg cactttacaa tttatgtatc tgatgaaaca cttggtacag
     5761 ttttacatag actatcagaa aaacagcgta gtgcgatttt attacgttat tttcaaggaa
     5821 tgaacgacag agaaatttca gaactgtacc atgtatcacg ttctgctatt tgcagtagaa
     5881 gaactagagg actaaggaag ttaaaaatgc ttttgagcga aaggaagtaa agattatggc
     5941 tatccctctt ttgaattgat ttgcaaagcg tcaagcggag atgaaatggc agttaaagag
     6001 attttgaagt tttatgacgg ttatatttct aaattatgct tgcgtccttt ttaccactct
     6061 gaaagtggta aaatcattat gcaggttgat gaagaactaa aaggagaaat tcatacagac
     6121 atgatgaaag caatattgaa atttgaaatc ggagtgaagt aatcaactgt ataaaaaaat
     6181 cgggagatac acagtttcgt gttatctccc ttagtagttt tacggtcata tcaaggctca
     6241 ttcaatcgtg gtaaattcgt ggtaagatga gtgtttttct atacttcaaa atgcttgaat
     6301 gtatagtgtt tatgcggata attgaaaatt agatataaaa tttaataaaa accaaaaatg
     6361 cacggtcgcc atgaagggac cgtgtatttt tatcttctta ttttctcacc taatcgtatc
     6421 agccgcatcg cttgttttcc tgcacaaaca agcaatcgct ctacctgttc catcgcataa
     6481 gaaagtacca tcggttcatt gatgatatca agtacatagt cgataccacc ctcacttaat
     6541 tgagcttgat ttttcttctc catattatgt agttgtggga caactttctg acaatcagca
     6601 attgtgtcaa gaacggatca atcctttatt ggagttgctc gaaagggtct tctcttatta
     6661 cggacaggct ttgctcactg tacatagaca acaaatcatt atcctagtca atcggatttc
     6721 cagagcctca ttgctatcat tgcttgataa aatccagaca aagtttaata aaatgtatca
     6781 gttacagcta aactttggaa ttgggtcgct ttgttataca gagcaagaga caccgcaatc
     6841 tttccttcat gccaaacaag tttgcgaatg gatagctttc catcaatctg tgaacgagat
     6901 ccgctttttt gaagatttag acctcgggat cgttttacca gcaatcccgt cagatcaacg
     6961 aacattatat gtaaaacgta ttttgaaaag cctgacagaa gaagaagtac atttgttcaa
     7021 gaagactttg gcatgcttca gtaaaaataa cggaagcatc aaaaattgct cagaagaatt
     7081 atttattcat aaaaacacct tgcaatatcg attgaacaag ttccattctc tcactggtta
     7141 tacccctaga aactatgacg attatcatat tctaaagctg gcttttttgt tggtacaaac
     7201 atgacaacta caaaaaaacc aggcgaacat atgattgttc gtctggtttt taattattaa
     7261 tgacttagcg tttttactag aaaaactact tccgcttttt cccgccaaaa gcatctttaa
     7321 acttatcgaa gaaaccgtct tcttgctgtt cttcaacttt gatgccgcca gcttcagcga
     7381 atgcacgtaa tgcttctctt tgttgttcat ttagattttt cggtgtgatc agttttactt
     7441 tgacatgttg gtcgcctgtt gcaccaccac gtaacttcgg agcgcctttt ccacgtaaac
     7501 ggaagttcgt acctgtctgt gttccagcag ggatcttcaa tttcacatct ccatggacag
     7561 ttggtacttg gacttcatcc cccaaagctg cttgtacaaa acttaaaggc aattcatagt
     7621 aaatttcaga accttcacga tcaaagatat cactttcttc tacacggaag accacataca
     7681 aatcaccaaa tggtccgccg ttttctccag cttctccttg accagctaaa cgcatttgtt
     7741 gtccttcttc tacaccagct ggtacattca ctttaactga atgtgctttc ttctcatgtc
     7801 ctgagccatg acaggttgga caaggttctt tgatttcttt ccctgtccca tgacaaacat
     7861 cacaagtttg acggctcatc actcgtccaa gtggtgtttg gcgttctaca ttaatcgtac
     7921 ctgcaccatg acatttatga caagtagttg gttgtgtgcc tggttttgca ccgttcccac
     7981 cacaagtatg acagatttct tcccgattat acttgatttc tttttctaca ccaaaaatcg
     8041 cttcttcaaa agacaagtcg accgtatatt gcaaatcagc accttgacga ggcgcatttg
     8101 gatcgactga ccgaccgcct cctccaaaga aagagtcgaa gatatcttca aaaccaccga
     8161 agccgccacc agagaagccg ccgaatccac caaatccgcc accagctcca ccagcaccgt
     8221 aatttggatc tgtgcctgca tgcccgtatt ggtcatatgc agcgcgtttt tgcgggtcac
     8281 ttaaaatctc atatgcttct gatacttctt tgaatttttc ttccgcatca ggttctttat
     8341 tgatgtcagg atggtatttt ttcgaaagct tacgataagc tttctttatt tcatcttctg
     8401 atgctccttt tgagagccct aagacttcat aataatcacg ttttgttgcc ataggctttc
     8461 ctcctattgg ttctcatcat tttcacgtcg cttgttatca tatcatattt tgtttgaaac
     8521 aaaaaccgat acttcaaact atttttcaaa agaaaaagcc aaagtagcaa ctttggcctt
     8581 ctctcgaaat tcagtgaact gattatttat cgtcgccatc gacttcttca aaatcagcat
     8641 caacgacatc atctgctccg ccttgagctg cttctgggtt ttcttgagct tgttgttgtg
     8701 ccgcttgttc gtatagttta acagtcaagt tttgaacgat ttcgttcaat gcatcgcgtt
     8761 tagttttcat ttcttcgatg ttgtttgctt caacagcggc tttcaattca tcgcgagctg
     8821 tttctgcttt tttcacttct tcttcgtcca ctttgccttc taattctttc aatgttttgt
     8881 ccacagagaa tagcaatgca tctacatcat tacgtaggtc aacttcctct ttacgagctt
     8941 tatctgcttc tgcatttgct tctgcatctt ttaccatacg ttcgatttct tcgtctgtta
     9001 gacctgaaga agatttgatc gtgatttttt gttctttttg tgtacctaga tctttcgcag
     9061 ataca
//
LOCUS       DASJTM010000307         8032 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.114, whole genome shotgun sequence.
ACCESSION   DASJTM010000307 DASJTM010000000
VERSION     DASJTM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.114"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..310)
                     /locus_tag="U4B29_003184"
     CDS             complement(<1..310)
                     /locus_tag="U4B29_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HEN2108041.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     A"
     gene            complement(652..1524)
                     /locus_tag="U4B29_003185"
     CDS             complement(652..1524)
                     /locus_tag="U4B29_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2108042.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1652..2221
                     /locus_tag="U4B29_003186"
     CDS             1652..2221
                     /locus_tag="U4B29_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2108043.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            2288..3025
                     /locus_tag="U4B29_003187"
     CDS             2288..3025
                     /locus_tag="U4B29_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2108044.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            3256..4524
                     /locus_tag="U4B29_003188"
     CDS             3256..4524
                     /locus_tag="U4B29_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2108045.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            5011..5475
                     /locus_tag="U4B29_003189"
     CDS             5011..5475
                     /locus_tag="U4B29_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2108046.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            5459..6088
                     /locus_tag="U4B29_003190"
     CDS             5459..6088
                     /locus_tag="U4B29_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2108047.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            6442..7920
                     /gene="msr(C)"
                     /locus_tag="U4B29_003191"
     CDS             6442..7920
                     /gene="msr(C)"
                     /locus_tag="U4B29_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2108048.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7967..>8032
                     /locus_tag="U4B29_003192"
     CDS             7967..>8032
                     /locus_tag="U4B29_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2108049.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLG"
BASE COUNT         2699 a         1433 c         1604 g         2296 t
ORIGIN      
        1 ccgcagaaaa agcaatgtgg tcggtcaaag cgatcaatac atgttcattc aacttttcca
       61 tcaaaacagt ttccgcatga tcaatgatcg tttctgctgc aaataaaaag cgttcatcaa
      121 tctgattaag taaattttgc aacttgattt gaccttctgg ttccatgaca aatatccgtt
      181 cgacatcacg atcaaaaatc aaatcatttc tttttctgtc aaatccaatc ccttttccaa
      241 tagctacttt ttcctgtcca ccgtcatcta ccaatactgc attttgatta aagatctttt
      301 tgattttcat gcgcgcaccc tttttgaaga tttggttttc acgtttgcta gaccagttga
      361 ttgcggccaa acaaaaatag acacgaaaaa cctcctaggg aaaccatgtt cctcagagat
      421 ttttcatgcc tagtatgatc tagtcacatg aaacgaatta ttgttaacgt aatcaatttt
      481 atatagacag ttatgttttg tcaattcttt ttttataatt atagtaattt actggaatta
      541 tcagaaataa aaggaaggaa caaattaaga aacttatgtt atttaatcat tggatctttt
      601 gcttaactta ttcgttgtta aaaagctgaa agcgaaggaa aaaaactcta cttaactgta
      661 tttctccgtt aggacatcta aaaatttacg agcagcagga gaaagaggct ggtctttttt
      721 ccaaatgaga ttcagtgaag cttgtaatgg cggatcgagt gggatgaata ctttttccgt
      781 cccataagta ttgatgatcc catctagagc taatgcacca aaagtcccgt tatcgaccat
      841 caaagaagca ttgtacaata agttatatgt tccgacgatc gataactgat ctatgctgaa
      901 acctagccat tcagctagtt gactgtccac tgacgtttgc cgggaaatga tcaaaggaac
      961 agttttcagt tcctgcggat ggatgactgt ttgctttgct aaaggatgat tccgatgtat
     1021 caaaagtccc caacgatctt gggctggcag tcgaaatgac tggtattttt gtttgtttgt
     1081 cggttcgatt acgagtccaa aatcaagtaa tccgctatcc agtttgtcta tgatatcatc
     1141 tgcattcccg ctatataaat gaaactggat atcaggatgc gccttgatca acgaatgaag
     1201 gctatcagaa atgaaagaga ttgctttagt ttcgcctccg ccaatgtaga tttctccaga
     1261 gatgattgcg tcatttgtta aattgttcgc cgttttttct accaacgaca agatttcttt
     1321 tccttttgaa taaagatatc gaccagaatc cgtcaactgt atctctcggg agcctcgagt
     1381 gaaaagtgtc acaccaagtt cttcttctaa atctttcagt tgttttgaga gagtaggttg
     1441 cgtgatatgc agagattttg ctgctcggct gatatttttt tctctagcta ctgctaaaaa
     1501 atacgtcagt atacgaatat ccattttttc cctcctgttt ttaaaaaagc ataccactaa
     1561 gtatagttaa aaggaatagt taagtatgtt ttataagtat tagacatcat aagccgtccg
     1621 ttataaagta aggataaaag gagggagcaa aatgacaagc ctcatcgaaa aacaaatcgt
     1681 caataaagag attcgagtaa cggatccgct ctttgaagtg atccatcaga tccaagcgga
     1741 aaacgaagag cctttagcag cgctgaatac tgggtaccat gaaccagcag atatccgcaa
     1801 acggctagaa aatatcatta gtgacaaagt agatgatacg gtgactgttc tgctgccttt
     1861 ttatacagat ttcggcaaac atattagtat agggaaaaat gtctttatca atcgtcaagt
     1921 gatgttcgtt gacttaggtg gcatctgttt agaagattca gtattgattg gtcctcgtgt
     1981 caacctcatc acggtgaacc atctaaccga tccaaaagaa cgaagaggct tgtcagtaaa
     2041 gccaatccac atcaagaaaa atgcttggat cggagcagga gcaactattc tgccaggcgt
     2101 aacgattggc gagaatgcca ttgttgcagc gaatgcaact gttacgaaag atgtcccaga
     2161 caataccatc gttgcaggaa tccctgccaa aatcgtcaaa cctgtcgaac ggattatttg
     2221 aacagtaaga tgaaataaga gatagtgagt ataagcaaga gatttttcaa aacaggagga
     2281 gaatagaatg aatgatttat cagaaaaagt gattgtcatc atgggcgctt caagcggaat
     2341 cggtgaagca acaacgaagc tattagcaga aaaaggggct aaactagtta tagctgcacg
     2401 ccgagaagat cgcctaaaag caataaaaga atctttacca gaagctgagc tatatattca
     2461 aacagcagat gtaagagatt ttgcacaagt ccaagcagtt atcgatttag cgatggaaaa
     2521 gttcggtcga atcgatgtat tgtacaataa tgcaggaatc atgccaaccg ctccattagt
     2581 tgaaggtcac cgtgatgaat ggcaaaatat gcttgatatc aacatcatgg gtgtgttaaa
     2641 tggaatttcc gccgtacttc caatcatgga aaaacaaaaa tctgggcata tcatttcaac
     2701 tgattctgtg gcgggacatg ttgtttatcc tgattccgcc gtttattgcg gaacaaaatt
     2761 tgctgtccgt gcaatcatgg aaggattacg tcaagagcaa cggcaaaata acatcaaatc
     2821 tacgatcatc tctccaggag ctgttcaaac tgaattatat caaaccatca gcgacaaaaa
     2881 ggctgctctt gaacttcatg aagcgcaaaa agaatgggga ttaacatctg aagatatagc
     2941 ttctgctgta gcatttgcga tagaaacgcc agaccggatg tctgtcagca acatgattat
     3001 ccgtccgacg acacaggaag tataatccat aacggataat atccagagag aattacttgt
     3061 taaatagtta ttcaaacgac catcaattta aggcgtgaat ggagaagaat attcttcatt
     3121 tacgtctttt tatgcagaaa aagcaaattt attaaaaaga aacaaatgtt atttaaatat
     3181 ggacattgac ttgcttttac ttttttagtt acactgttat ttaggaaaag tttcataatt
     3241 caaaggagaa aaaagatgaa agcaaagaat ttattattta gcgttattct tttaggaaat
     3301 ctagcagttg ccaacacaac agtgctagct gaagaatcga cagaaactgt cgcaactcag
     3361 acatcgagtt cttcagaaac aggaattgaa aacgatacag aaagtacttt gcagtcagct
     3421 actgtctcag aaaatacaat ttcaaccgaa gaaccaacaa ctgaaacaac agaacaaaca
     3481 caaaccgaag acagtacatc agcagaagct cagaacaatc cagagaatgc agaagaaatc
     3541 acgaaccaat tgacagatgg gcaaacattg acacataccc atcaagcaga tttgtctgaa
     3601 ttcgaaaact atactggcta tgctttttat gctcgctcat ctgctaattc tcaacaagcg
     3661 ttcattgata gtattgcctc aactgcacaa agtttggcaa gtgccaatga cttatatgca
     3721 tcagtcatga ttgctcaagc catcgttgaa agtggctggg ggaacagtac tttggcttct
     3781 gcccctaatt acaatctatt tgggattaaa ggaagctata atggccaatc tgttacgatg
     3841 cctacttcgg aatatgtgaa tgggcaatgg attacggtga atgccgcttt tagaaaatat
     3901 ccttcgtaca aagaatcatt gcaagataac gtaacggtat tgaagacgac ttcttttcag
     3961 ccaggcgttt actactattc cggtgcatgg aaaagcaata cgaactctta caaagatgct
     4021 acggcttggc taacaggtcg ttatgccact gcgcctaatt atggttctac attgaacaat
     4081 gtaatcgaaa cctataatct gacacagtat gatacagcac caacttcaac ttctccgatg
     4141 tatcgtctgt ataaccgtca tacaggagaa catttatata cattgaatgc cggagaaaaa
     4201 aattatctgc caactgtagg ctgggaatat gaagggattg cttggcaagc accaaacagc
     4261 ggacaacctg tctaccgttt atacaatcct tacagtggag accatcatta cacaatggca
     4321 caatctgaga tcaactcttt aacaaaaatc ggttggcgtt atgaaggtct aagtttttat
     4381 tctggtggtt ccaagccaat ttatcgacta tttaatccaa atgaaaaaac tggtacccat
     4441 cactatacct tgagtgctaa ggaaagagat tggctaacac cgatgggctg gagatatgaa
     4501 ggaataggat tttatggtta ttaaaaagag gtagtgaaaa agctgccctg catcaagtag
     4561 taagaacagc caaacaaaaa cagattttca tgttttttgt ttggttgaac ttattaccgc
     4621 agatgcaagt ttttgaacac cgtttactca aaaaaggttg tgacaacatc ttgtcataac
     4681 ttttttatta ctgtttaagc atactgatcc aatttctaat attcgacggg attcttgcaa
     4741 aaggcgattt cggaaatttt tggcggtgta aaattatcta ctgcagaaga gctatcttct
     4801 atagtttgat aagaatcaat aatcttggtt ttagcgttgt gctttcttta tcattttttc
     4861 cgttcattct acaaaaaatt tcttttctct atacttttct attgagagcg ttttttttat
     4921 tacacagctg attctttgac ttttttcttt ttttattcta ttattgacaa gtcaatcgtt
     4981 gacgagtcaa ttattagaaa ggaggaaatt atgtctttag aagagattag tcacctttta
     5041 tatcaaatca aattagccga tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata
     5101 agtcttacta gatacgagat catgatgaga ctgaaagaag aaaatccatt gctacaaagc
     5161 agtttgcaag aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg
     5221 gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta
     5281 gcgctaacag aaaaagccca gaagaaaatc aagcattgtg aaatggacca taaagataca
     5341 ggttccgtgc tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta
     5401 gataaattga atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat
     5461 ggaacaaaaa aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga
     5521 tgaaacatac aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc
     5581 accttcttct gtcaatatga aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa
     5641 agaaatcttg cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat
     5701 ggtcatgatt tttggcgata tgcaatgtta cgaatatggc gaagaaatct acaaccaagc
     5761 ttacgaatca gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc
     5821 ttattacaag agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt
     5881 agcggctatg caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg
     5941 tggattcgaa gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc
     6001 agttattatt ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc
     6061 accagaaaag atcactatgt tcgaataaaa ggttttcagt tactattcag tagctattgc
     6121 tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg
     6181 atgaaattac gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa
     6241 caacaatttc atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc
     6301 tatgacagat ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg
     6361 cagttttttt gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca
     6421 tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc
     6481 agctttggca accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt
     6541 atagggatca taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt
     6601 gaactttttc ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa
     6661 ttaacctatc tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta
     6721 agactggaag aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg
     6781 atacgtcttc tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta
     6841 gatagaaaaa gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt
     6901 tttgttagtc atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa
     6961 gacggagaaa tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg
     7021 gagaaaaaga ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa
     7081 aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag
     7141 aaaaagcaac aaatcagact ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa
     7201 aaagccatcc aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca
     7261 accaagccgc agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac
     7321 agccgctatc ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg
     7381 gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt
     7441 tcaggtaaga caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct
     7501 ccgaaagtaa gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc
     7561 atcattcgtt ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg
     7621 aatcacttag ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga
     7681 gaagcgaccc gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta
     7741 gatgaaccga ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa
     7801 atatatccgg gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct
     7861 gatgaagttt atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa
     7921 atgtgctacg atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa
     7981 aaaagaagga ataattttta caacaatgat gtgttttttg atggtcttgg gg
//
LOCUS       DASJTM010000308         5622 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.142, whole genome shotgun sequence.
ACCESSION   DASJTM010000308 DASJTM010000000
VERSION     DASJTM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.142"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            211..1107
                     /locus_tag="U4B29_003193"
     CDS             211..1107
                     /locus_tag="U4B29_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2108050.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1257..2198)
                     /locus_tag="U4B29_003194"
     CDS             complement(1257..2198)
                     /locus_tag="U4B29_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2108051.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2324..3445
                     /locus_tag="U4B29_003195"
     CDS             2324..3445
                     /locus_tag="U4B29_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2108052.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3703..5205
                     /gene="eat(A)"
                     /locus_tag="U4B29_003196"
     CDS             3703..5205
                     /gene="eat(A)"
                     /locus_tag="U4B29_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2108053.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5272..>5622
                     /locus_tag="U4B29_003197"
     CDS             5272..>5622
                     /locus_tag="U4B29_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2108054.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLD"
BASE COUNT         1860 a         1001 c         1178 g         1583 t
ORIGIN      
        1 atttaacaat gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt
       61 aggcgaattt taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt
      121 tcatacaaat ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga
      181 aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca
      241 aaaaataaat cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa
      301 gatttactac agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg
      361 atcgtttttt tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc
      421 aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg
      481 aagcaggcac aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca
      541 aaagaacttg ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct
      601 agcgaaccaa agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca
      661 gaaagctata agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca
      721 catcgaggga atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg
      781 gaatcgcgcg ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca
      841 tttgaatcca tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat
      901 catttttatc gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg
      961 gaatttctgc atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca
     1021 gaaaattggg caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat
     1081 gacctctcct ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa
     1141 acaaacaagc acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt
     1201 gggcttaaaa aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat
     1261 tctgcgccaa ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt
     1321 gtagcaatat catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca
     1381 gtcaaacgat ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg
     1441 tcaggatcat aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct
     1501 tcttttgctg cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct
     1561 ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt
     1621 ccatttacac aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt
     1681 aaatttgctt cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc
     1741 aagttctcta gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga
     1801 ccagctgtcc acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt
     1861 acatcttcat gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg
     1921 gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag
     1981 tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc
     2041 acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca
     2101 ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg
     2161 ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa
     2221 tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa
     2281 atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc
     2341 aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg
     2401 ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac
     2461 tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct
     2521 cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc
     2581 agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac
     2641 tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga
     2701 agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt
     2761 actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac
     2821 taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc
     2881 tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca
     2941 aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca
     3001 gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg
     3061 cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt
     3121 cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga
     3181 agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc
     3241 aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg
     3301 actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga
     3361 agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag
     3421 atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt
     3481 caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct
     3541 gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt
     3601 ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag
     3661 ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata
     3721 aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta
     3781 aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact
     3841 ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa
     3901 tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat
     3961 gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat
     4021 cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta
     4081 ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat
     4141 ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc
     4201 atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc
     4261 gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa
     4321 cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta
     4381 aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag
     4441 aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct
     4501 gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc
     4561 agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc
     4621 caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat
     4681 ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt
     4741 cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag
     4801 gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat
     4861 gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca
     4921 tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag
     4981 cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct
     5041 gaactatata catgggatga accattgaat tatttggatg tcttcaatca agaacaatta
     5101 gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc
     5161 ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa
     5221 gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat
     5281 gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat
     5341 gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat
     5401 ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca
     5461 caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg
     5521 gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta
     5581 gcgttcgctg tacgattccc aaatggggag tcagtcttag at
//
LOCUS       DASJTM010000309         2529 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.197, whole genome shotgun sequence.
ACCESSION   DASJTM010000309 DASJTM010000000
VERSION     DASJTM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.197"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            262..759
                     /gene="dfrG"
                     /locus_tag="U4B29_003198"
     CDS             262..759
                     /gene="dfrG"
                     /locus_tag="U4B29_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2108055.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(831..>2529)
                     /locus_tag="U4B29_003199"
     CDS             complement(831..>2529)
                     /locus_tag="U4B29_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2108056.1"
                     /translation="DVKGKPSRYKMTGSSKDKGHKGMYCNDNPIGRGVSQNCTVTPLS
                     NWSVVEEIKRLIEINSIQDVEPDYQFHKEGCSEEESTPFNEPKQFYKRGKSRGKSQRY
                     QCKACKKFTNVLPKREETTTYHQQKNTILPMFAKMVVGRVSVSRTCDILGIGVGTYYH
                     KLEWLYRRCLEFLERYETQPLQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYTGFED
                     LNMQTYIVVSAEVLSRYVFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDW
                     SYYPQEPSANDSENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADE
                     WRMISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDW
                     GDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKG
                     FYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSY
                     IYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          795 a          418 c          445 g          871 t
ORIGIN      
        1 tttggagtac aaaatgacaa aatgtacttc aaaatggact acttttttca ttttaactta
       61 cataccaaat tacatacaaa atgatttata aaatgcaatt gaaaatgata attgaatgaa
      121 aatttgtgat tttgtggtat tatgataata taacttatag aaaaaattgc ttattcaact
      181 aacgggcagg ataatgagaa aaggtttttt gatagaaata agaaattaga tggtaaatta
      241 gttaaaaagg ctgtgttata aatgaaagtt tctttgattg ctgcgatgga taagaataga
      301 gtgattggca aagagaatga cattccttgg aggattccca aggactggga atatgttaaa
      361 aatactacaa agggacatcc gataatatta ggtaggaaga accttgaatc aatcggaaga
      421 gccttacctg acagaagaaa tattattctg acgagagata aggggtttac ctttaatggt
      481 tgtgaaattg ttcattcaat agaagatgtt tttgagttat gtaaaaacga agaagaaatt
      541 tttattttcg gaggagaaca gatttataat ttgtttttcc cttatgttga gaaaatgtac
      601 atcacaaaaa tacatcatga attcgaagga gatacttttt ttccagaagt gaattatgag
      661 gaatggaatg aggtatttgc ccaaaaaggg ataaagaatg ataaaaatcc gtataactac
      721 tattttcatg tatatgaaag aaaaaactta ttgagttaac gggtgcgtta attgaagaag
      781 aacagtaaag ataaagcaca agagcttggg attactccaa gctctttttg tcatttgaaa
      841 taaattatat ctttccaatc aaattgttta tctgtaatcc ctaatctttg agcaggtgtc
      901 aaaacctttt tatccgctga tttaaaaggg cgacaaaagt tgtagtaagt tcttagaatt
      961 gtaatcgcaa attgagcata cttcggattg aagttagcgt aaatatagct cttcttatca
     1021 ccacgagctg ttgttaatgg tctttcaaga gatgatatat aacgtcttat ctgttgaata
     1081 aagctattgg tcgaatggtc attaacattt aatagcatct ttgcgatttc gtttggttcc
     1141 agagcagata aatccgttct acaatcaact gaataaaatc ctttatcctt agtggctaat
     1201 ggatgtttga tagggttatc ggcatatttg cgatatcttt ctccatcttt actaatagcc
     1261 tcttcgtgga attgatgact tgttagtaat tcagttagat aatgagatgc caaagtccgt
     1321 agaaactttg ttttgaatcc tctgatatct ccccaatcga gcagttctgc ttttgcttgt
     1381 ccgaattctt ttaagcattg ttttctgctt ttcgtcttgt taacttggca aataaaatga
     1441 tgtgcatcgc tcaaccttaa ttctttcgtg aatactctat aaaaggcgtt tctaatagag
     1501 aagtcatcat cactaatcat tcgccattcg tcggcattaa ccatttgttt aattaaccaa
     1561 tagtgagcag ttgtagtata aggagcatca acatttaaac catcgacaaa tctactccta
     1621 ttagtaattt tccctaattc gtgaagataa gcattcctat tttcgctatc gttagcggat
     1681 ggttcttgtg gataataaga ccaatctaac ctatcatttt tacgactaaa agtattgaga
     1741 tggtcttctt tgaattttct tgtatcttca tttaattcat ccattgaaat attccaatca
     1801 taggcaacat cagaacggaa cacatatctt gataagacct cagcagatac aactatatac
     1861 gtttgcatat taaggtcttc aaaccccgta tatttctttg aaccttgtcc tttcttacgg
     1921 acgttattta ggtagtagtg catcttgtcc gtattaagcc acatctcgtt aaatttcttt
     1981 gtttgaaggg gttgtgtctc atatcgttct aaaaactcca aacaacgcct atacagccac
     2041 tccagcttat gatagtaggt tccaactcca attcctaaaa tatcacaagt acgactgact
     2101 gaaacccttc caacaaccat cttagcgaac attggaagaa tggtgttctt ttgttggtga
     2161 taagtagtgg tttcttctct ttttggtaga acattagtga acttcttaca cgccttacat
     2221 tgatagcgtt gagatttgcc cctacttttc ccccttttgt aaaattgctt cggctcattg
     2281 aatggggttg attcttcttc cgagcagcct tctttatgaa attgataatc tggctcaaca
     2341 tcttggatgc tattgatttc tataaggcgt ttaatttctt caacaacaga ccaattagat
     2401 aaaggggtta ctgtacagtt ttgcgataca cctctgccaa taggattgtc attgcagtac
     2461 atacctttat gacctttatc tttggaacta ccagtcattt tatatcgact tggtttccct
     2521 tttacatct
//
LOCUS       DASJTM010000310         2136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.210, whole genome shotgun sequence.
ACCESSION   DASJTM010000310 DASJTM010000000
VERSION     DASJTM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.210"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..1166
                     /locus_tag="U4B29_003200"
     CDS             12..1166
                     /locus_tag="U4B29_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2108057.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1381..>2136
                     /locus_tag="U4B29_003201"
     CDS             1381..>2136
                     /locus_tag="U4B29_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2108058.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGA"
BASE COUNT          649 a          409 c          499 g          579 t
ORIGIN      
        1 ggtatggggg gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac
       61 gaaaacttta catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata
      121 ttcgttcaat gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat
      181 atgacttaaa tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag
      241 atatctttat ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt
      301 caaaattcgc aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg
      361 aagataaaca aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat
      421 taaaacggac tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg
      481 acgttgttat gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt
      541 tgagcctgct tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata
      601 tcacgttgga caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac
      661 ggtataacct acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg
      721 tgcagatgac cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc
      781 acgcccccga ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca
      841 acattttgaa aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg
      901 gcctctccgg ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata
      961 agctagctgc catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg
     1021 gtggcgcggg acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga
     1081 tttacgcgga aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag
     1141 acttggttga taaaaggagg tcctaagaga tgtatataat tttttaggaa aatctcaagg
     1201 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
     1261 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
     1321 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
     1381 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
     1441 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
     1501 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
     1561 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
     1621 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
     1681 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
     1741 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
     1801 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
     1861 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
     1921 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
     1981 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
     2041 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
     2101 gttaaagcat tagaaaacgg gaaactgggc ggtgcc
//
LOCUS       DASJTM010000311         1828 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.221, whole genome shotgun sequence.
ACCESSION   DASJTM010000311 DASJTM010000000
VERSION     DASJTM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.221"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..632
                     /locus_tag="U4B29_003202"
     CDS             <1..632
                     /locus_tag="U4B29_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2108059.1"
                     /translation="YLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYN
                     LQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNN
                     ILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSD
                     TGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            847..1815
                     /locus_tag="U4B29_003203"
     CDS             847..1815
                     /locus_tag="U4B29_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2108060.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT          545 a          364 c          437 g          482 t
ORIGIN      
        1 gttatttgag cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg
       61 tgcatatcac gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga
      121 ttacacggta taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata
      181 tgctggtgca gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg
      241 ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct
      301 ttaacaacat tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta
      361 ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa
      421 aagataagct agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg
      481 atacgggtgg cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag
      541 ggcagattta cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga
      601 tgccagactt ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc
      661 tcaaggttat ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt
      721 tcttacacgg tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt
      781 tgacaagatt accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc
      841 ttaattatga ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca
      901 ttccatgctc tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg
      961 gaatccaacg ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag
     1021 atttccgcct ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga
     1081 agcatcggct gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac
     1141 aatgtggcgt actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca
     1201 gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc
     1261 gaccgtggca aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc
     1321 aaagcggtta ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc
     1381 cgaagtatag aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt
     1441 acgcttcatg tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag
     1501 agaatgaagc aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat
     1561 gagttggtta aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa
     1621 ggagaggaag agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta
     1681 cttaaacttc aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag
     1741 caagcgttgc gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga
     1801 caggagcatg aatagaataa aagttgca
//
LOCUS       DASJTM010000312         1729 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.226, whole genome shotgun sequence.
ACCESSION   DASJTM010000312 DASJTM010000000
VERSION     DASJTM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.226"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..1051
                     /gene="vanA"
                     /locus_tag="U4B29_003204"
     CDS             20..1051
                     /gene="vanA"
                     /locus_tag="U4B29_003204"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN2108061.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            1057..1665
                     /locus_tag="U4B29_003205"
     CDS             1057..1665
                     /locus_tag="U4B29_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN2108062.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          517 a          329 c          430 g          453 t
ORIGIN      
        1 tttgaaagga gacaggagca tgaatagaat aaaagttgca atactgtttg ggggttgctc
       61 agaggagcat gacgtatcgg taaaatctgc aatagagata gccgctaaca ttaataaaga
      121 aaaatacgag ccgttataca ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa
      181 accttgcgcg gaatgggaaa acgacaattg ctattcagct gtactctcgc cggataaaaa
      241 aatgcacgga ttacttgtta aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc
      301 attttcagct ttgcatggca agtcaggtga agatggatcc atacaaggtc tgtttgaatt
      361 gtccggtatc ccttttgtag gctgcgatat tcaaagctca gcaatttgta tggacaaatc
      421 gttgacatac atcgttgcga aaaatgctgg gatagctact cccgcctttt gggttattaa
      481 taaagatgat aggccggtgg cagctacgtt tacctatcct gtttttgtta agccggcgcg
      541 ttcaggctca tccttcggtg tgaaaaaagt caatagcgcg gacgaattgg actacgcaat
      601 tgaatcggca agacaatatg acagcaaaat cttaattgag caggctgttt cgggctgtga
      661 ggtcggttgt gcggtattgg gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat
      721 caggctgcag tacggaatct ttcgtattca tcaggaagtc gagccggaaa aaggctctga
      781 aaacgcagtt ataaccgttc ccgcagacct ttcagcagag gagcgaggac ggatacagga
      841 aacggcaaaa aaaatatata aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt
      901 tttacaagat aacggccgca ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc
      961 atacagtcgt tatccccgta tgatggccgc tgcaggtatt gcacttcccg aactgattga
     1021 ccgcttgatc gtattagcgt taaaggggtg ataagcatgg aaataggatt tactttttta
     1081 gatgaaatag tacacggtgt tcgttgggac gctaaatatg ccacttggga taatttcacc
     1141 ggaaaaccgg ttgacggtta tgaagtaaat cgcattgtag ggacatacga gttggctgaa
     1201 tcgcttttga aggcaaaaga actggctgct acccaagggt acggattgct tctatgggac
     1261 ggttaccgtc ctaagcgtgc tgtaaactgt tttatgcaat gggctgcaca gccggaaaat
     1321 aacctgacaa aggaaagtta ttatcccaat attgaccgaa ctgagatgat ttcaaaagga
     1381 tacgtggctt caaaatcaag ccatagccgc ggcagtgcca ttgatcttac gctttatcga
     1441 ttagacacgg gtgagcttgt accaatgggg agccgatttg attttatgga tgaacgctct
     1501 catcatgcgg caaatggaat atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc
     1561 atcatggaaa acagtgggtt tgaagcatat agcctcgaat ggtggcacta tgtattaaga
     1621 gacgaaccat accccaatag ctattttgat ttccccgtta aataaacttt taaccgttgc
     1681 acggacaaac ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DASJTM010000313         1603 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.230, whole genome shotgun sequence.
ACCESSION   DASJTM010000313 DASJTM010000000
VERSION     DASJTM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.230"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..112
                     /locus_tag="U4B29_003206"
     CDS             <1..112
                     /locus_tag="U4B29_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2108063.1"
                     /translation="NQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            145..1053
                     /locus_tag="U4B29_003207"
     CDS             145..1053
                     /locus_tag="U4B29_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN2108064.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1050..1530
                     /locus_tag="U4B29_003208"
                     /pseudo
     CDS             1050..1530
                     /locus_tag="U4B29_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          563 a          230 c          361 g          449 t
ORIGIN      
        1 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
       61 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
      121 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
      181 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
      241 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
      301 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
      361 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
      421 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
      481 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
      541 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
      601 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
      661 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
      721 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
      781 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
      841 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
      901 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
      961 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     1021 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     1081 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     1141 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     1201 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     1261 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     1321 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     1381 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     1441 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     1501 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     1561 ttgtggttct gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DASJTM010000314         1273 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.251, whole genome shotgun sequence.
ACCESSION   DASJTM010000314 DASJTM010000000
VERSION     DASJTM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.251"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..1171
                     /locus_tag="U4B29_003209"
     CDS             260..1171
                     /locus_tag="U4B29_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN2108065.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          455 a          187 c          258 g          373 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gac
//
LOCUS       DASJTM010000315         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.258, whole genome shotgun sequence.
ACCESSION   DASJTM010000315 DASJTM010000000
VERSION     DASJTM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.258"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..76
                     /locus_tag="U4B29_003210"
     CDS             11..76
                     /locus_tag="U4B29_003210"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2108066.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="U4B29_003211"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="U4B29_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2108067.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DASJTM010000316          983 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.267, whole genome shotgun sequence.
ACCESSION   DASJTM010000316 DASJTM010000000
VERSION     DASJTM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.267"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..472
                     /gene="vanZ"
                     /locus_tag="U4B29_003212"
     CDS             11..472
                     /gene="vanZ"
                     /locus_tag="U4B29_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN2108068.1"
                     /translation="MLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTPFTATGNFR
                     EMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGATDITDVIT
                     NTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINYV"
BASE COUNT          334 a          143 c          170 g          336 t
ORIGIN      
        1 atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt
       61 acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt
      121 tactgctact gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg
      181 cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact
      241 ggttttaagt cttacttttg aaataattca atttatcttc gctattggag cgacagacat
      301 aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag
      361 caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt
      421 gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta
      481 aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga ttagatggaa
      541 aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt
      601 aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag
      661 gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc
      721 cccactagca gccatgcttt ttttgaatct ttgtaggacg ttttaaagag aattacccac
      781 tttaaaatta aacatgcgct tagaatcgct ttaggaaaca cgatccagtc caataatcgt
      841 cgataaaaac ttttgaaaaa ggttggtgaa attacctact tttggaataa tcacaaatca
      901 caagtgatta atcacttgtt tattaagata ttaaaagcta taatttaaat aaagcgtgaa
      961 ttttattaca caaaaagagg ggg
//
LOCUS       DASJTM010000317          861 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.280, whole genome shotgun sequence.
ACCESSION   DASJTM010000317 DASJTM010000000
VERSION     DASJTM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.280"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(673..>861)
                     /locus_tag="U4B29_003213"
     CDS             complement(673..>861)
                     /locus_tag="U4B29_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2108069.1"
                     /translation="SHSLNFLAEVDEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVE
                     YEKSLYPRKKVRQDERIN"
BASE COUNT          262 a          169 c          133 g          297 t
ORIGIN      
        1 gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta attgttaaat
       61 accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca ttatatccag
      121 aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg tcgaataaac
      181 tgccaaagac atacaatcat attgctttta aaattgacga acaagatata gattcgtttg
      241 tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg
      301 aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta cataccggta
      361 ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa tgactagtct
      421 ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg aattgatctg
      481 ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt catcacaaat
      541 acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc tcgtgtgggt
      601 tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat ttttgtctca
      661 gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg ggtatagact
      721 tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc tcacaacaaa
      781 ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt ctacttcagc
      841 taggaagttc aaactatggg a
//
LOCUS       DASJTM010000318          840 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.281, whole genome shotgun sequence.
ACCESSION   DASJTM010000318 DASJTM010000000
VERSION     DASJTM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.281"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            124..672
                     /gene="aac(6')"
                     /locus_tag="U4B29_003214"
     CDS             124..672
                     /gene="aac(6')"
                     /locus_tag="U4B29_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2108070.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          303 a          126 c          188 g          223 t
ORIGIN      
        1 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
       61 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
      121 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
      181 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
      241 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
      301 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
      361 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
      421 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
      481 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
      541 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
      601 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
      661 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
      721 atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg aagaagctac
      781 ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
//
LOCUS       DASJTM010000319          793 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2910
            SAMN38756313-rid20573373.guided.287, whole genome shotgun sequence.
ACCESSION   DASJTM010000319 DASJTM010000000
VERSION     DASJTM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756313
            Sequence Read Archive: SRR27167964
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028129.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:09:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,215
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 35 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756313-rid20573373.guided.287"
                     /isolate="VRE2910"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-11-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="U4B29_003215"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="U4B29_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN2108071.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT          271 a          149 c          166 g          207 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaa
//
